CN110904009A - Microbial preparation for treating excrement of anhydrous ecological toilet and treatment method thereof - Google Patents

Microbial preparation for treating excrement of anhydrous ecological toilet and treatment method thereof Download PDF

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CN110904009A
CN110904009A CN201911297484.XA CN201911297484A CN110904009A CN 110904009 A CN110904009 A CN 110904009A CN 201911297484 A CN201911297484 A CN 201911297484A CN 110904009 A CN110904009 A CN 110904009A
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fermentation
filler
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ecological toilet
excrement
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余小舟
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Jiangsu Baobang Technology Development Co Ltd
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Biochemistry (AREA)
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Abstract

The invention relates to the technical field of fecal treatment, in particular to a microbial preparation for fecal treatment of an anhydrous ecological toilet and a treatment process thereof, and the microbial preparation comprises the following steps: s1: raw material acquisition: s2: and (4) preparation. The invention can effectively absorb and decompose the rich nutrient elements and the like contained in the human excrement by mixing multiple strains and fermenting the filler.

Description

Microbial preparation for treating excrement of anhydrous ecological toilet and treatment method thereof
Technical Field
The invention relates to the technical field of excrement treatment, in particular to an excrement treatment microbial preparation for an anhydrous ecological toilet and a treatment process thereof.
Background
The waterless ecological toilet is a toilet of environmental protection type, which can not pollute the environment, can fully utilize various resources, does not need traditional hydraulic flushing, emphasizes the concepts and functions of pollutant self-purification and resource recycling, has better energy-saving and environment-friendly effects, the most available current waterless ecological toilet is a toilet for decomposing excrement by microorganism bacteria, and utilizes the growth and reproduction activities of the waterless ecological toilet to biodegrade and convert the macromolecular organic compounds which can be utilized in the excrement into bacterial biomass, competitively inhibit and kill pathogenic microorganisms in the excrement, adsorb, degrade and convert odor substances generated in the excrement, realize harmless and resource treatment of the excrement, can achieve the function of zero discharge, can not cause any pollution to the environment at all, and has the effect of saving water resources.
The microbial treatment is the foremost generation of treatment technology so far, but the technical limitation is also obvious, for example, the disclosed document (CN109880773A) discloses a compound microbial preparation for efficiently decomposing human and animal excreta and a preparation method thereof, the effect of cellulase and protease subjected to high-temperature treatment is poor after the cellulase and the protease are mixed for use, the activity of the prepared total bacterial colony is low, the capability of treating waste excreta is limited, the environmental requirements of fermentation and growth are high, the strain treatment range is limited and is not suitable for most regions, the continuous and effective action in multi-strain and severe environments is difficult, the treatment effect expectation is reduced, the fermented excreta generates more volatile ammonia, the odor of the ecological toilet is serious, and the large-scale popularization is difficult.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a microbial preparation for treating excrement of an anhydrous ecological toilet and a treatment process thereof.
The invention provides a microbial composition for treating feces in an anhydrous ecological toilet, which comprises the following components in gram:
bacillus subtilis (30-60) hundred million CFU, Rhizobium (60-80) hundred million CFU, Bacillus subtilis (30-60) hundred million CFU, Bacillus subtilis (60-80) hundred million CFU, Bacillus subtilis (30-60) and Bacillus subtilis (60-80) or Bacillus subtilis (60-60) or,
Phanerochaete chrysosporium (50-80) hundred million CFU,
Pediococcus acidilactici (Pediococcus acidilactici) 20-40 hundred million CFU (CFU), Rhodotorula rubra (Rhodotorula) 20-30 hundred million CFU
Bifidobacterium (20-30 hundred million CFU)
Bacillus mucilaginosus (Bacillus mucilaginosus) 20-30 CFU
Glucose was used as a carrier to make up to 1g in total.
The excrement treatment process for preparing the composition by using the microorganisms for the excrement treatment of the anhydrous ecological toilet comprises the following steps of:
s1: the preparation method of the fermentation filler comprises the following steps of: fully mixing 8-15% of soybean straw powder, 20-30% of coconut shell powder, 15-25% of xylo-oligosaccharide, 8-10% of coprinus atramentarius dry powder, 3-5% of betaine, 1-2% of monopotassium phosphate, 0.5-1.2% of magnesium sulfate, 0.5-1.2% of ferric sulfate and the balance of purified water, adjusting the pH value to 6.8-7.2, and fully and uniformly stirring to obtain a mixture for later use;
s2: sterilizing and dissolving the fermentation filler, injecting the fermentation filler into a fermentation tank, introducing high-temperature steam, and cooling the fermentation filler;
s3: inoculating and preparing a fermentation mixture, namely adding a microorganism composition into a fermentation tank, adding 2-4 parts of strain liquid into 100 parts of fermentation filler by volume, stirring to uniformly mix the strain liquid and the fermentation filler, and fermenting for 10-12 hours to obtain a fermentation raw material;
s4: and (3) treating the excrement, namely mixing the fermentation raw material extracted from S3 with excrement 1: (30-35) adding and mixing the materials in a mass ratio, and heating to 40-55 ℃ for continuous fermentation.
Further, in S2, the temperature in the fermentation tank is 150-250 ℃, and the fermentation filler is sterilized after being kept for 1-3 hours.
Further, the strain liquid and the fermentation filler in the fermentation tank need to be stirred at the stirring speed of 60-90 r/min.
Further, the temperature in the fermentation tank in S3 is controlled to be 25-35 ℃.
Further, pH was 6.8 in the fermenter in S3.
The invention has the following beneficial effects:
the invention can effectively absorb and decompose rich nutrient elements and the like contained in human excrement by multi-strain mixing and fermentation of the filler, the coconut shell powder, the coprinus atramentarius dry powder and the xylo-oligosaccharide in the filler can provide basic sugar and necessary amino acid for the strains, the weak alkalinity in betaine can inhibit the rapid growth of nitrogen fixing bacteria, the suppression of other strains can be prevented, necessary nutrient factors can be provided for other strains, the potassium dihydrogen phosphate, the magnesium sulfate and the ferric sulfate provide trace elements, the nitrogen which is fixed into a free state immediately is synthesized by the effective matching of the filler and the microorganism bacteria, the odor is greatly reduced, the technical problem that volatile indole, skatole, hydrogen carbide, hydrogen sulfide, amine and other odors are easily generated in the conventional excrement fermentation treatment is solved, the effective fermentation time can be effectively prolonged by the strains, the effective fermentation time can be improved by more than 20 percent, the fermentation efficiency is improved, the effect is continuously and effectively exerted, the method is suitable for being matched with an anhydrous ecological toilet to carry out fermentation decomposition operation of excrement, the finished product can be recovered into an organic fertilizer, the application effect of the prepared finished product is good, the nitrogen content is high, the environmental pollution is reduced, and the method has great economic value.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
The microbial compositions of examples 1-4 were selected as follows, and each gram of the composition consisted of the following components:
bacillus subtilis 40 hundred million CFU,
Rhizobia (Rhizobium)70 hundred million CFU,
Phanerochaete chrysosporium (Phanerochaete chrysosporium)60 hundred million CFU,
Pediococcus acidilactici (Pediococcus acidilactici)30 hundred million CFU,
Pichia pastoris (Rhodotorula)30 hundred million CFU
Bifidobacterium (Bifidobacterium)30 million CFU
Bacillus mucilaginosus (Bacillus mucoarginosus) 30CFU
Glucose was used as a carrier to make up to 1g in total.
The above strains are all commonly purchased strains in the market.
Stool treatment example:
example 1
S1: the preparation method of the fermentation filler comprises the following steps of: fully mixing 15% of soybean straw powder, 20% of coconut shell powder, 15% of xylo-oligosaccharide, 10% of coprinus atramentarius dry powder, 5% of betaine, 2% of monopotassium phosphate, 0.5% of magnesium sulfate, 0.5% of ferric sulfate and the balance of purified water, adjusting the pH value to 7.2, and fully and uniformly stirring to obtain a mixture for later use; the strain liquid and the fermentation filler in the fermentation tank need to be stirred at the stirring speed of 60r/min, and the temperature in the fermentation tank is controlled at 35 ℃;
s2: sterilizing and dissolving the fermentation filler, injecting the fermentation filler into a fermentation tank, introducing high-temperature steam, and cooling the fermentation filler; the temperature in the fermentation tank is 250 ℃, and the filler is sterilized after being kept for 1 hour;
s3: inoculating and preparing a fermentation mixture, namely adding a microorganism composition into a fermentation tank, adding 2 parts of strain liquid into 100 parts of fermentation filler by volume, stirring to uniformly mix the strain liquid and the fermentation filler, and fermenting for 12 hours to obtain a fermentation raw material; pH 6.8 in fermenter in S3
S4: and (3) treating the excrement, namely mixing the fermentation raw material extracted from S3 with excrement 1: 30, and heating to 55 ℃ for continuous fermentation.
Example 2
S1: the preparation method of the fermentation filler comprises the following steps of: fully mixing 8% of soybean straw powder, 30% of coconut shell powder, 25% of xylo-oligosaccharide, 8% of coprinus atramentarius dry powder, 3% of betaine, 1% of monopotassium phosphate, 0.5% of magnesium sulfate, 0.5% of ferric sulfate and the balance of purified water, adjusting the pH value to 7.2, and fully and uniformly stirring to obtain a mixture for later use; the stirring speed is 90 r/min;
s2: sterilizing and dissolving the fermentation filler, injecting the fermentation filler into a fermentation tank, introducing high-temperature steam, and cooling the fermentation filler; in the S2, the temperature in the fermentation tank is 150 ℃, and the fermentation filler is sterilized after being kept for 3 hours;
s3: inoculating and preparing a fermentation mixture, namely adding a microorganism composition into a fermentation tank, adding 4 parts of strain liquid into 100 parts of fermentation filler by volume, stirring to uniformly mix the strain liquid and the fermentation filler, and fermenting for 12 hours to obtain a fermentation raw material; in S3, the temperature in the fermentation tank is controlled at 35 ℃ and the pH value is 6.8;
s4: and (3) treating the excrement, namely mixing the fermentation raw material extracted from S3 with excrement 1: 35, mixing, heating to 50 ℃, and continuously fermenting.
Example 3
S1: the preparation method of the fermentation filler comprises the following steps of: fully mixing 10% of soybean straw powder, 25% of coconut shell powder, 20% of xylo-oligosaccharide, 9% of coprinus atramentarius dry powder, 4% of betaine, 1.5% of monopotassium phosphate, 0.8% of magnesium sulfate, 0.8% of ferric sulfate and the balance of purified water, adjusting the pH value to 6.8, and fully and uniformly stirring to obtain a mixture for later use; the stirring speed is 80 r/min;
s2: sterilizing and dissolving the fermentation filler, injecting the fermentation filler into a fermentation tank, introducing high-temperature steam, and cooling the fermentation filler; in the S2, the temperature in the fermentation tank is 200 ℃, and the fermentation filler is sterilized after being kept for 2 hours;
s3: inoculating and preparing a fermentation mixture, namely adding a microorganism composition into a fermentation tank, adding 3 parts of strain liquid into 100 parts of fermentation filler by volume, stirring to uniformly mix the strain liquid and the fermentation filler, and fermenting for 12 hours to obtain a fermentation raw material; the temperature in the fermenter in S3 was controlled at 30 ℃ and pH 6.8.
S4: and (3) treating the excrement, namely mixing the fermentation raw material extracted from S3 with excrement 1: 32, and heating to 48 ℃ for continuous fermentation.
Example 4
S1: the preparation method of the fermentation filler comprises the following steps of: fully mixing soybean straw powder 12%, coconut shell powder 26%, xylo-oligosaccharide 18%, coprinus atramentarius dry powder 10%, betaine 3%, monopotassium phosphate 2%, magnesium sulfate 1.2%, ferric sulfate 1.2% and the balance of purified water, adjusting the pH value to 6.8-7.2, and fully and uniformly stirring to obtain a mixture for later use; the stirring speed is 70 r/min;
s2: sterilizing and dissolving the fermentation filler, injecting the fermentation filler into a fermentation tank, introducing high-temperature steam, and cooling the fermentation filler;
s3: inoculating and preparing a fermentation mixture, namely adding a microorganism composition into a fermentation tank, adding 2-4 parts of strain liquid into 100 parts of fermentation filler by volume, stirring to uniformly mix the strain liquid and the fermentation filler, and fermenting for 10-12 hours to obtain a fermentation raw material; the temperature in the fermentation tank is 180 ℃, and the fermentation filler is sterilized after being kept for 2 hours; the temperature in the fermentation tank is controlled at 30 ℃, and the pH value in the fermentation tank is 6.8;
s4: and (3) treating the excrement, namely mixing the fermentation raw material extracted from S3 with excrement 1: 34, and heating to 50 ℃ for continuous fermentation.
The test effects of examples 1 to 4 are as follows:
Figure BDA0002320933090000071
the recovery of the prepared finished product in the embodiments 1-4 detects that the finished product contains rich trace elements and active bacteria components:
the experimental results are tabulated below:
Figure BDA0002320933090000072
the above is a table comparing the effects of examples 1-4, acting on soybeans, and using it to obtain:
the finished product plays an important role in the aspects of mineralization of organic matters, accumulation of nutrient elements, synthesis of humus and the like, under the action of active bacteria microorganisms, organic nutrients are continuously decomposed and converted into effective nutrients which can be absorbed and utilized by plants, meanwhile, certain nutrients fixed in soil can be released, a large amount of gibberellin, cell hormones and other substances can be generated in the microbial propagation process, and after the substances are contacted with plant roots, the metabolism of crops can be regulated, the crop roots can grow vigorously, organic acids, antibacterial peptides and the like can be secreted, the invasion and the propagation opportunities of pathogenic bacteria can be inhibited and reduced, the organic acids or certain organic matter groups generated by decomposition of the finished product in the soil are complexed with iron and aluminum, and the fixation of phosphorus by the soil is reduced; the soil reducibility is improved, so that iron and aluminum are in a reduction state to improve the solubility of phosphorus; increase the effectiveness of trace elements such as zinc, manganese, iron and the like in the soil. Compared with the common nitrogen fertilizer, the finished product can increase the yield of the soybean by more than 20 percent.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (6)

1. The microbial composition for treating the excrement of the anhydrous ecological toilet is characterized by comprising the following components in gram:
bacillus subtilis (30-60) hundred million CFU,
Rhizobium (60-80) hundred million CFU,
Phanerochaete chrysosporium (50-80) hundred million CFU,
Pediococcus acidilactici (Pediococcus acidilactici) 20-40 hundred million CFU,
Pichia pastoris (Rhodotorula) 20-30 hundred million CFU
Bifidobacterium (20-30 hundred million CFU)
Bacillus mucilaginosus (Bacillus mucilaginosus) 20-30 CFU
Glucose was used as a carrier to make up to 1g in total.
2. A fecal treatment process based on the microorganism-made composition for fecal treatment of the waterless ecological toilet according to any of claims 1 to 3, characterized by comprising the steps of:
s1: the preparation method of the fermentation filler comprises the following steps of: fully mixing 8-15% of soybean straw powder, 20-30% of coconut shell powder, 15-25% of xylo-oligosaccharide, 8-10% of coprinus atramentarius dry powder, 3-5% of betaine, 1-2% of monopotassium phosphate, 0.5-1.2% of magnesium sulfate, 0.5-1.2% of ferric sulfate and the balance of purified water, adjusting the pH value to 6.8-7.2, and fully and uniformly stirring to obtain a mixture for later use;
s2: sterilizing and dissolving the fermentation filler, injecting the fermentation filler into a fermentation tank, introducing high-temperature steam, and cooling the fermentation filler;
s3: inoculating and preparing a fermentation mixture, namely adding a microorganism composition into a fermentation tank, adding 2-4 parts of strain liquid into 100 parts of fermentation filler by volume, stirring to uniformly mix the strain liquid and the fermentation filler, and fermenting for 10-12 hours to obtain a fermentation raw material;
s4: and (3) treating the excrement, namely mixing the fermentation raw material extracted from S3 with excrement 1: (30-35) adding and mixing the materials in a mass ratio, and heating to 40-55 ℃ for continuous fermentation.
3. The microbial preparation for the fecal treatment of the waterless ecological toilet and the treatment process thereof according to claim 1, characterized in that: and S2, sterilizing the fermentation filler by keeping the temperature in the fermentation tank at 150-250 ℃ for 1-3 hours.
4. The treatment process of the microbial preparation for the fecal treatment of the waterless ecological toilet according to claim 1, characterized in that the strain liquid and the fermentation filler in the fermentation tank need to be stirred at a speed of 60-90 r/min.
5. The treatment process of the microbial preparation for the fecal treatment of the waterless ecological toilet according to the claim 1, characterized in that: and S3, controlling the temperature in the fermentation tank to be 25-35 ℃.
6. The treatment process of the microbial preparation for the fecal treatment of the waterless ecological toilet according to the claim 1, characterized in that: and the pH value in the fermentation tank is 6.8 in S3.
CN201911297484.XA 2019-12-17 2019-12-17 Microbial preparation for treating excrement of anhydrous ecological toilet and treatment method thereof Pending CN110904009A (en)

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WO2017161091A1 (en) * 2016-03-16 2017-09-21 Spogen Biotech Inc. Methods for promoting plant health using free enzymes and microorganisms that overexpress enzymes
CN105906169A (en) * 2016-06-30 2016-08-31 内蒙古阜丰生物科技有限公司 Biological preparation for treating sodium glutamate fermentation sewage
CN106699332A (en) * 2016-11-17 2017-05-24 广西黎塘远东化肥有限责任公司 Production method of special fertilizer for watermelon
CN110029073A (en) * 2019-03-13 2019-07-19 广东工业大学 A kind of method of feces of livestock and poultry high-efficiency aerobic compost bacterium and its aerobic compost

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