CN110846312B - 一种sdaA基因的启动子核酸序列、含有该核酸序列的重组菌株及其应用 - Google Patents
一种sdaA基因的启动子核酸序列、含有该核酸序列的重组菌株及其应用 Download PDFInfo
- Publication number
- CN110846312B CN110846312B CN201910937089.7A CN201910937089A CN110846312B CN 110846312 B CN110846312 B CN 110846312B CN 201910937089 A CN201910937089 A CN 201910937089A CN 110846312 B CN110846312 B CN 110846312B
- Authority
- CN
- China
- Prior art keywords
- promoter
- nucleotide sequence
- recombinant strain
- lysine
- recombinant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 101150100082 sdaA gene Proteins 0.000 title abstract description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract description 11
- 150000007523 nucleic acids Chemical group 0.000 title abstract description 11
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims abstract description 73
- 239000004472 Lysine Substances 0.000 claims abstract description 38
- 235000019766 L-Lysine Nutrition 0.000 claims abstract description 36
- 238000000855 fermentation Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 17
- 230000035772 mutation Effects 0.000 claims abstract description 13
- 239000002773 nucleotide Substances 0.000 claims description 41
- 125000003729 nucleotide group Chemical group 0.000 claims description 41
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 29
- 239000012634 fragment Substances 0.000 claims description 26
- 239000013612 plasmid Substances 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 19
- 108020004414 DNA Proteins 0.000 claims description 18
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 14
- 241000186226 Corynebacterium glutamicum Species 0.000 claims description 8
- 238000012408 PCR amplification Methods 0.000 claims description 8
- 108091026890 Coding region Proteins 0.000 claims description 7
- 229940104302 cytosine Drugs 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 229930024421 Adenine Natural products 0.000 claims description 6
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 6
- 238000012220 PCR site-directed mutagenesis Methods 0.000 claims description 6
- 229960000643 adenine Drugs 0.000 claims description 6
- 241001485655 Corynebacterium glutamicum ATCC 13032 Species 0.000 claims description 5
- 238000010276 construction Methods 0.000 claims description 5
- 230000001965 increasing effect Effects 0.000 claims description 4
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 2
- 230000002194 synthesizing effect Effects 0.000 claims description 2
- 230000001131 transforming effect Effects 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000186254 coryneform bacterium Species 0.000 claims 1
- 101150001481 sda gene Proteins 0.000 claims 1
- 238000012986 modification Methods 0.000 abstract description 3
- 230000004048 modification Effects 0.000 abstract description 3
- 150000008545 L-lysines Chemical class 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 description 10
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 229930027917 kanamycin Natural products 0.000 description 5
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
- 229960000318 kanamycin Drugs 0.000 description 5
- 229930182823 kanamycin A Natural products 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000001976 enzyme digestion Methods 0.000 description 4
- 238000010353 genetic engineering Methods 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 2
- 241000572303 Corynebacterium pekinense Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000002744 homologous recombination Methods 0.000 description 2
- 230000006801 homologous recombination Effects 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000012257 pre-denaturation Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186031 Corynebacteriaceae Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (14)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910937089.7A CN110846312B (zh) | 2019-09-29 | 2019-09-29 | 一种sdaA基因的启动子核酸序列、含有该核酸序列的重组菌株及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910937089.7A CN110846312B (zh) | 2019-09-29 | 2019-09-29 | 一种sdaA基因的启动子核酸序列、含有该核酸序列的重组菌株及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110846312A CN110846312A (zh) | 2020-02-28 |
CN110846312B true CN110846312B (zh) | 2020-11-10 |
Family
ID=69596151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910937089.7A Active CN110846312B (zh) | 2019-09-29 | 2019-09-29 | 一种sdaA基因的启动子核酸序列、含有该核酸序列的重组菌株及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110846312B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111909944A (zh) * | 2020-06-08 | 2020-11-10 | 内蒙古伊品生物科技有限公司 | 一种lysC基因改造的重组菌株及其构建方法与应用 |
CN115449519B (zh) * | 2021-06-08 | 2023-04-07 | 中国科学院天津工业生物技术研究所 | 基于dapB基因的具有启动子活性的多核苷酸及其用途 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014121669A1 (zh) * | 2013-02-08 | 2014-08-14 | 宁夏伊品生物科技股份有限公司 | 用改变乌头酸酶基因和/或其调控元件的细菌发酵生产l-赖氨酸的方法 |
CN106318964A (zh) * | 2016-09-01 | 2017-01-11 | 宁夏伊品生物科技股份有限公司 | 棒杆菌发酵生产l‑赖氨酸的方法及其启动子改造 |
-
2019
- 2019-09-29 CN CN201910937089.7A patent/CN110846312B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014121669A1 (zh) * | 2013-02-08 | 2014-08-14 | 宁夏伊品生物科技股份有限公司 | 用改变乌头酸酶基因和/或其调控元件的细菌发酵生产l-赖氨酸的方法 |
CN106318964A (zh) * | 2016-09-01 | 2017-01-11 | 宁夏伊品生物科技股份有限公司 | 棒杆菌发酵生产l‑赖氨酸的方法及其启动子改造 |
Non-Patent Citations (2)
Title |
---|
CP025533.1;GenBank;《GenBank》;20171227;"FEATURES"部分 * |
The Actinobacterium Corynebacterium glutamicum, an Industrial Workhorse;Joo-Young Lee等;《J. Microbiol. Biotechnol.》;20160203;第26卷(第5期);第807-822页 * |
Also Published As
Publication number | Publication date |
---|---|
CN110846312A (zh) | 2020-02-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110607313B (zh) | 一种高产l-赖氨酸的重组菌株及其构建方法与应用 | |
CN110195087B (zh) | 用改变ppc基因的细菌发酵生产L-赖氨酸的方法 | |
CN111850010B (zh) | 一种dapB基因改造的重组菌株及其构建方法与应用 | |
CN111909944A (zh) | 一种lysC基因改造的重组菌株及其构建方法与应用 | |
CN110846312B (zh) | 一种sdaA基因的启动子核酸序列、含有该核酸序列的重组菌株及其应用 | |
WO2022143761A1 (zh) | 一种改造基因bbd29_04920的产l-谷氨酸的重组菌株及其构建方法与应用 | |
JP2024505808A (ja) | 増強されたl-グルタミン酸生産能を有する菌株及びその構築方法と応用 | |
CN111471693B (zh) | 一种产赖氨酸的谷氨酸棒杆菌及其构建方法与应用 | |
CN110846333B (zh) | 一种deoB基因改造的重组菌株及其构建方法与应用 | |
CN110592109B (zh) | 一种spoT基因改造的重组菌株及其构建方法与应用 | |
CN110592084B (zh) | 一种rhtA基因启动子改造的重组菌株及其构建方法与应用 | |
CN111961635A (zh) | 一种产l-赖氨酸的重组菌株及其构建方法与应用 | |
CN114540399B (zh) | 一种制备l-缬氨酸的方法及其所用基因突变体和生物材料 | |
WO2022143646A1 (zh) | 一种改造基因bbd29_09525产l-谷氨酸的重组菌株及其构建方法与应用 | |
WO2022143639A1 (zh) | 一种改造基因bbd29_11265产l-谷氨酸的重组菌株及其构建方法与应用 | |
CN110804617B (zh) | 一种kdtA基因改造的重组菌株及其构建方法与应用 | |
CN114181288A (zh) | 制备l-缬氨酸的方法及其所用的基因与该基因编码的蛋白质 | |
CN114315998B (zh) | Cey17_rs00300基因突变体及其在制备l-缬氨酸中的应用 | |
CN114277069B (zh) | 制备l-缬氨酸的方法及其所用生物材料 | |
JP2023527951A (ja) | L-リジン産生組換え菌株、その構築方法及び使用 | |
CN114539367A (zh) | Cey17_rs11900基因突变体及其在制备l-缬氨酸中的应用 | |
JP2022547010A (ja) | 大腸菌に基づく組換え株ならびにその構築方法および使用 | |
CN114410615A (zh) | Yh66_00525蛋白及其编码基因在调控细菌精氨酸产量中的应用 | |
CN114605509A (zh) | Yh66_01475蛋白及其编码基因在调控细菌精氨酸产量中的应用 | |
EP3992294A1 (en) | Escherichia coli-based recombinant strain and construction method therefor and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A DNA sequence of sdaa gene promoter, a recombinant strain containing the DNA sequence and its application Effective date of registration: 20210127 Granted publication date: 20201110 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2021640000001 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220129 Granted publication date: 20201110 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2021640000001 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A promoter nucleic acid sequence of sdaa gene, a recombinant strain containing the nucleic acid sequence and its application Effective date of registration: 20220323 Granted publication date: 20201110 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2022640000002 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230315 Granted publication date: 20201110 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd.|Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2022640000002 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A promoter nucleic acid sequence of sdaA gene, recombinant strain containing the nucleic acid sequence, and its application Granted publication date: 20201110 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd.|Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2024640000005 |