CN110839521A - Planting method of dendrobium pohuanense rich in scoparone - Google Patents
Planting method of dendrobium pohuanense rich in scoparone Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
Abstract
The invention relates to the technical field of planting of dendrobium candidum, in particular to a planting method of dendrobium candidum rich in scoparone, which comprises the following steps: 1) and (3) treatment of the cultivation container: sequentially paving manganese ore slag, potassium-containing rock and phosphogypsum in a cultivation container, then adding corn stalk powder into the cultivation container, irradiating for 30-45min under purple light, and finally adding magnetized water into the cultivation container; 2) cuttage: placing the nursery stock in a cultivation container, laying the wheat straw subjected to steam explosion treatment, and placing the cultivation container under natural conditions for planting; by adopting the planting method, the seedling root system is strong, the survival rate is high, the contents of scoparone, coumarin and chlorophyll are high, the reasonable utilization of solid waste is facilitated, the operation is easy, no fertilizer application or additional fertilizer is needed, and the labor intensity is reduced.
Description
Technical Field
The invention relates to the technical field of planting of dendrobium candidum, in particular to a planting method of dendrobium candidum rich in scoparone.
Background
Herba Dendrobii is a variety of Dendrobium loddigesii Rolfe of Dendrobium of Orchidaceae, and is also one of the famous and precious medicinal materials in Chuanshengdao. The dendrobium medicinal materials collected in the 2010 edition of the Chinese pharmacopoeia are mainly from dendrobium nobile lindl, dendrobium chrysotoxum, dendrobium fimbriatum, dendrobium candidum and fresh or dry stems of similar species of the same genus. The dendrobium nobile is sweet in taste and slightly cold in nature, and has the effects of tonifying stomach, promoting the production of body fluid, nourishing yin and clearing heat. It is used clinically to treat fever with body fluid deficiency, dry mouth, polydipsia, stomach yin deficiency, anorexia, retching, persistent asthenic fever after illness, hyperactivity of fire due to yin deficiency, bone steaming, overstrain, dim eyesight, flaccidity of tendons and bones. Modern pharmacological studies show that the dendrobium poecilomikovii has obvious effects of resisting tumors, reducing blood sugar, enhancing immunity and the like.
At present, researches on planting methods of dendrobium candidum are few, and a uniform planting standard is not formed, so that the survival rate of the dendrobium candidum is low, the content of effective components is low, and the popularization and the application of the dendrobium candidum are limited. For example, scoparone is used as one of the effective components of the Mailuoning injection, can inhibit platelet aggregation, delay thrombosis and reduce the quality of thrombus, but the content of scoparone in the herba dendrobii is low, and the content of scoparone extracted from the herba dendrobii is reported by Zhang Lei et al in the document Bihai lactone content determination in the plant of dendrobe 4 and is measured to be 31.8 mug/g, so that the improvement of the scoparone in the herba dendrobii is favorable for the popularization and application of the herba dendrobii. For another example, the coumarins have the effects of relaxing smooth muscles, dilating blood vessels and resisting blood coagulation, and the average content of coumarins (0.623mg/g) of dendrobium candidum is determined by the established reverse high performance liquid chromatography in the document 'determination of content of coumarins in dendrobium candidum by RP-HPLC' by Zhou Xiao Mei et al, so that the establishment of a scientific planting method of dendrobium candidum is necessary.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides a planting method of dendrobium congolense rich in scoparone, which comprises the following specific steps:
a planting method of dendrobium pohuanense rich in scoparone comprises the following steps:
1) and (3) treatment of the cultivation container: sequentially paving 8-12mm thick manganese slag, 10-14mm thick potassium-containing rock and 6-10mm thick phosphogypsum in a cultivation container, adding 50-70% of cornstalk powder by mass of the manganese slag into the cultivation container, irradiating for 30-45min under purple light, and finally adding 30-50% of magnetized water by mass of the cornstalk powder into the cultivation container;
2) cuttage: placing the nursery stock in a cultivation container, laying a layer of wheat straw with the thickness of 2-6mm after steam explosion treatment, and placing the cultivation container in a natural condition for planting.
According to the invention, the dendrobium can be planted by utilizing the manganese slag, the potassium-containing rock and the phosphogypsum, so that the effective utilization of solid waste is realized, meanwhile, the corn stalk powder is utilized, the release of water and nutrients can be regulated, the root rot or water shortage is avoided, the growth of the dendrobium can be facilitated by adding the magnetized water, the accumulation of effective components in the dendrobium can be facilitated by regulating the components of the magnetized water, and the contents of components such as scoparone and coumarin are further improved; this application lays the one deck wheat straw after nursery stock cuttage, can play the heat preservation effect of moisturizing, prevents that the weather is overheated or crosses the temperature that brings excessively, and then has realized planting not receive season climate change's restriction. By controlling the particle size of the material, the ventilation of the root system and the permeation of oxygen are ensured, and oxygen deficiency is prevented.
The nursery stock cultivation step is to lay nursery stocks on the ground in order, press pretreated barks on the nursery stocks and place the nursery stocks for 10 to 20 days under natural conditions; can promote the growth of the root system of the nursery stock, lead the root of the nursery stock to be sturdy and be beneficial to the accumulation of chlorophyll, and further improve the environmental adaptability and the stress resistance of the nursery stock.
The pretreatment of the bark is to mix the bark according to the proportion of 1: (2.6-4.6) soaking the raw materials in a complex enzyme solution for 20-40min according to the mass ratio of the raw materials to the raw materials, and then mixing the raw materials according to the weight ratio of 1: (1.7-3.7) soaking in 30-50% sodium sulfide solution for 60-150min, and mixing with ultrasonic wave with frequency of 25-35kHz, taking out bark, and mixing the above materials at a ratio of 1: (1.4-2.1) soaking the material liquid in the urea/silicon dioxide composite solution at 35-45 ℃ for 45-75 min.
The complex enzyme solution per ml contains 1900-.
The concentration of the urea/silicon dioxide solution is 37-67%, and the mass ratio of urea to silicon dioxide is 1: (0.3-0.9).
The invention utilizes the compound enzyme solution to process, so that the cell structure of the bark is destroyed, and then utilizes the sodium sulfide to process, thus being beneficial to the dissolution of components such as lignin, cellulose, hemicellulose and the like, being assisted with ultrasonic waves to accelerate the dissolution, and enabling the dissolved components to have weak electromagnetic induction performance, thus being beneficial to being absorbed by the nursery stock, and utilizing the urea/silicon dioxide solution, not only controlling the release amount of the effective components dissolved from the bark, but also promoting the absorption of the nursery stock to nutrients and air.
The granularity of the manganese slag, the potassium-containing rock and the phosphogypsum is 30-40 meshes.
And spraying 1-5% of disodium ethylene diamine tetraacetate solution to the manganese slag, and then carrying out flame spraying treatment for 800 seconds for 300-800 seconds by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 5-9% of the mass of the manganese slag.
The potassium-containing rock is sprayed with 1-5% disodium ethylene diamine tetraacetate solution, and then is sprayed with flame spray gun for 300-800s, wherein the amount of the disodium ethylene diamine tetraacetate solution is 5-9% of the mass of the potassium-containing rock.
After 1-5% of disodium ethylene diamine tetraacetate solution is sprayed on the phosphogypsum, the phosphogypsum is sprayed for 800 seconds by a flame spray gun, and the dosage of the disodium ethylene diamine tetraacetate solution is 5-9% of the mass of the phosphogypsum.
According to the invention, the manganese slag, the potassium-containing rock and the phosphogypsum are treated by using the disodium ethylene diamine tetraacetate solution, and the solidified manganese element, potassium element, phosphorus element and sulfur element are quickly changed into free elements by combining with the flaming treatment, so that the absorption is facilitated.
The preparation method of the cornstalk powder comprises the following steps: mixing corn stalks with water according to the weight ratio of 1: (3.1-3.9), performing frequency reduction ultrasonic treatment, drying in a low-temperature vacuum environment at the temperature of-10-0 ℃, and grinding to 600 meshes; the step of the frequency reduction ultrasonic treatment is to treat the mixture for 35 to 60 seconds under the condition of the frequency of 126-142kHz, reduce the mixture to the frequency of 35 to 45kHz at the speed of 0.3 to 0.8kHz/min, and treat the mixture for 120-150 seconds at constant frequency.
Each liter of magnetized water contains 33-79mg of potassium, 54-107mg of phosphorus, 11-18mg of sulfur, 1-4mg of selenium, 0.01-0.04mg of europium and 1-4mg of allantoin.
The allantoin is added into the magnetized water, which is helpful for promoting cell growth and rapidly healing the mechanical damage suffered by the nursery stock in the cutting process.
The steam explosion treatment is to place 30-40 mesh wheat straw in a steam explosion machine under 2.3-3.7Mpa for 55-129 s.
The invention uses steam explosion technology to process the wheat straw, so that the starch in the wheat straw is puffed and gelatinized, the release rate of the effective components is further controlled, sunlight can be absorbed, and heat preservation and moisture content regulation are performed by using illumination.
Compared with the prior art, the invention has at least the following beneficial effects:
by adopting the planting method, the seedling root system is strong, the survival rate is high, the contents of scoparone, coumarin and chlorophyll are high, the reasonable utilization of solid waste is facilitated, the operation is easy, no fertilizer application or additional fertilizer is needed, and the labor intensity is reduced.
Detailed Description
The technical solution of the present invention is further defined below with reference to the specific embodiments, but the scope of the claims is not limited to the description.
Example 1
A planting method of dendrobium pohuanense rich in scoparone comprises the following steps:
1) and (3) treatment of the cultivation container: sequentially paving manganese ore slag with the thickness of 12mm, potassium-containing rock with the thickness of 14mm and phosphogypsum with the thickness of 10mm in a cultivation container, then adding cornstalk powder accounting for 70% of the mass of the manganese ore slag in the cultivation container, placing the container under purple light for irradiating for 45min, and finally adding magnetized water accounting for 50% of the mass of the cornstalk powder in the cultivation container;
2) cuttage: placing the nursery stock in a cultivation container, laying a layer of wheat straw with the thickness of 6mm after steam explosion treatment, and placing the cultivation container under natural conditions for planting;
the pretreatment of the bark is to mix the bark according to the proportion of 1: 4.6, soaking the mixture in a complex enzyme solution for 40min, and then mixing the mixture according to the mass ratio of 1: 3.7, soaking in 50% sodium sulfide solution for 150min, adding 35kHz ultrasonic wave, taking out bark, and mixing the above materials at a ratio of 1: 2.1, soaking the material liquid in a urea/silicon dioxide composite solution at the temperature of 45 ℃ for 75 min;
the complex enzyme solution per milliliter contains 2700U of cellulase, 1395U of pectinase, 2250U of protease and 1017U of tannase;
the concentration of the urea/silicon dioxide solution is 67%, and the mass ratio of urea to silicon dioxide is 1: 0.9;
the granularity of the manganese slag, the potassium-containing rock and the phosphogypsum is 40 meshes;
spraying a 5% disodium ethylene diamine tetraacetate solution to the manganese slag, and then carrying out flame spraying treatment for 800s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 9% of the mass of the manganese slag;
spraying a 5% disodium ethylene diamine tetraacetate solution to the potassium-containing rock, and then carrying out flame spraying treatment for 800s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 9% of the mass of the potassium-containing rock;
spraying a disodium ethylene diamine tetraacetate solution with the concentration of 5% on the phosphogypsum, and then carrying out flaming treatment for 800s by using a flame spray gun, wherein the dosage of the disodium ethylene diamine tetraacetate solution is 9% of the mass of the phosphogypsum;
the preparation method of the cornstalk powder comprises the following steps: mixing corn stalks with water according to the weight ratio of 1: 3.9, performing frequency reduction ultrasonic treatment, drying in a low-temperature vacuum environment at 0 ℃, and grinding to 600 meshes; the step of the frequency reduction ultrasonic treatment is that the treatment is carried out for 60s under the condition that the frequency is 142kHz, the treatment is carried out for 150s at a constant frequency by reducing the frequency to 45kHz at the speed of 0.8 kHz/min;
the magnetized water per liter contains 79mg of potassium, 107mg of phosphorus, 18mg of sulfur, 4mg of selenium, 0.04mg of europium and 4mg of allantoin;
the steam explosion treatment is to place the wheat straw with the grain size of 40 meshes in a steam explosion machine, wherein the explosion condition is 3.7Mpa and the explosion time is 129 s.
Example 2
A planting method of dendrobium pohuanense rich in scoparone comprises the following steps:
1) and (3) treatment of the cultivation container: sequentially paving 8 mm-thick manganese slag, 10 mm-thick potassium-containing rock and 6 mm-thick phosphogypsum in a cultivation container, adding 50% of cornstalk powder by mass of the manganese slag into the cultivation container, irradiating for 30min under purple light, and finally adding 30% of magnetized water by mass of the cornstalk powder into the cultivation container;
2) cuttage: placing the nursery stock in a cultivation container, laying a layer of wheat straw with the thickness of 2mm after steam explosion treatment, and placing the cultivation container under natural conditions for planting;
the pretreatment of the bark is to mix the bark according to the proportion of 1: 2.6, soaking the mixture in a complex enzyme solution for 20min, and then mixing the mixture according to the mass ratio of 1: 1.7, soaking in 30% sodium sulfide solution for 60min, and taking out bark according to the weight ratio of 1: 1.4, soaking the material liquid in urea/silicon dioxide composite solution at 35 ℃ for 45 min;
the complex enzyme solution per ml contains 1900U of cellulase, 1305U of pectinase, 2130U of protease and 960U of tannase;
the concentration of the urea/silicon dioxide solution is 37%, and the mass ratio of urea to silicon dioxide is 1: 0.3;
the granularity of the manganese slag, the potassium-containing rock and the phosphogypsum is 30 meshes;
spraying 1% of disodium ethylene diamine tetraacetate solution to the manganese slag, and then carrying out flame spraying treatment for 300s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 5% of the mass of the manganese slag;
spraying 1% disodium ethylene diamine tetraacetate solution to the potassium-containing rock, and then carrying out flame spraying treatment for 300s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 5% of the mass of the potassium-containing rock;
after spraying a 1% disodium ethylene diamine tetraacetate solution to the phosphogypsum, carrying out flaming treatment for 300s by using a flame spray gun, wherein the dosage of the disodium ethylene diamine tetraacetate solution is 5% of the mass of the phosphogypsum;
the preparation method of the cornstalk powder comprises the following steps: mixing corn stalks with water according to the weight ratio of 1: 3.1, performing frequency reduction ultrasonic treatment, drying in a low-temperature vacuum environment at the temperature of minus 10 ℃, and grinding to 600 meshes; the step of the frequency reduction ultrasonic treatment is that the treatment is carried out for 35s under the condition that the frequency is 126kHz, the treatment is carried out for 120s under the condition that the frequency is reduced to 35kHz at the speed of 0.3kHz/min, and the treatment is carried out for 120s under the constant frequency;
each liter of magnetized water contains 33mg of potassium, 54mg of phosphorus, 11mg of sulfur, 1mg of selenium, 0.01mg of europium and 1mg of allantoin;
the steam explosion treatment is to place the wheat straw with the granularity of 30 meshes in a steam explosion machine, wherein the explosion condition is 2.3Mpa, and the explosion time is 55 s.
Example 3
A planting method of dendrobium pohuanense rich in scoparone comprises the following steps:
1) and (3) treatment of the cultivation container: sequentially paving 12 mm-thick manganese slag, 10 mm-thick potassium-containing rock and 10 mm-thick phosphogypsum in a cultivation container, adding 57% of cornstalk powder by mass of the manganese slag into the cultivation container, irradiating for 38min under purple light, and finally adding 40% of magnetized water by mass of the cornstalk powder into the cultivation container;
2) cuttage: placing the nursery stock in a cultivation container, laying a layer of wheat straw with the thickness of 4mm after steam explosion treatment, and placing the cultivation container under natural conditions for planting;
the pretreatment of the bark is to mix the bark according to the proportion of 1: 3.6, soaking the mixture in a complex enzyme solution for 30min, and then mixing the mixture according to the mass ratio of 1: 2.7, soaking in 30% sodium sulfide solution for 90min, and taking out bark according to the weight ratio of 1: 1.9, soaking the material liquid in urea/silicon dioxide composite solution for 60min at the temperature of 39 ℃;
the complex enzyme solution per milliliter contains 2300U of cellulase, 1395U of pectinase, 2186U of protease and 998U of tannase;
the concentration of the urea/silicon dioxide solution is 51%, and the mass ratio of urea to silicon dioxide is 1: 0.6;
the granularity of the manganese slag, the potassium-containing rock and the phosphogypsum is 35 meshes;
spraying a disodium ethylene diamine tetraacetate solution with the concentration of 3% on the manganese slag, and then carrying out flame spraying treatment for 600s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 5% of the mass of the manganese slag;
spraying a disodium ethylene diamine tetraacetate solution with the concentration of 2.5% on the potassium-containing rock, and then carrying out flame spraying treatment for 500s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 9% of the mass of the potassium-containing rock;
spraying a 1% disodium ethylene diamine tetraacetate solution to the phosphogypsum, and then carrying out flaming treatment for 800s by using a flame spray gun, wherein the dosage of the disodium ethylene diamine tetraacetate solution is 6% of the mass of the phosphogypsum;
the preparation method of the cornstalk powder comprises the following steps: mixing corn stalks with water according to the weight ratio of 1: 3.6, performing frequency reduction ultrasonic treatment, drying in a low-temperature vacuum environment at the temperature of minus 5 ℃, and grinding to 600 meshes; the step of frequency reduction ultrasonic treatment is that the treatment is carried out for 45s under the condition that the frequency is 131kHz, the treatment is carried out for 135s at constant frequency by reducing the frequency to 40kHz at the speed of 0.5 kHz/min;
each liter of magnetized water contains 33mg of potassium, 107mg of phosphorus, 18mg of sulfur, 1mg of selenium, 0.01mg of europium and 4mg of allantoin;
the steam explosion treatment is to place the wheat straw with the grain size of 40 meshes in a steam explosion machine, wherein the explosion condition is 2.3Mpa and the explosion time is 129 s.
Example 4
A planting method of dendrobium pohuanense rich in scoparone comprises the following steps:
1) and (3) treatment of the cultivation container: sequentially paving 10 mm-thick manganese slag, 12 mm-thick potassium-containing rock and 8 mm-thick phosphogypsum in a cultivation container, adding 60% of cornstalk powder by mass of the manganese slag into the cultivation container, irradiating for 37min under purple light, and finally adding 40% of magnetized water by mass of the cornstalk powder into the cultivation container;
2) cuttage: placing the nursery stock in a cultivation container, laying a layer of wheat straw with the thickness of 4mm after steam explosion treatment, and placing the cultivation container under natural conditions for planting;
the pretreatment of the bark is to mix the bark according to the proportion of 1: 3.6, soaking the mixture in a complex enzyme solution for 30min, and then mixing the mixture according to the mass ratio of 1: 2.7, soaking in 40% sodium sulfide solution for 100min, adding 30kHz ultrasonic wave, taking out bark, and mixing the above solutions at a ratio of 1: 1.8, soaking the mixture in urea/silicon dioxide composite solution at 40 ℃ for 60 min;
the complex enzyme solution per milliliter contains 2300U of cellulase, 1350U of pectinase, 2190U of protease and 989U of tannase;
the concentration of the urea/silicon dioxide solution is 51%, and the mass ratio of urea to silicon dioxide is 1: 0.6;
the granularity of the manganese slag, the potassium-containing rock and the phosphogypsum is 35 meshes;
spraying a disodium ethylene diamine tetraacetate solution with the concentration of 3% on the manganese slag, and then carrying out flame spraying treatment for 500s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 7% of the mass of the manganese slag;
spraying a disodium ethylene diamine tetraacetate solution with the concentration of 3% on the potassium-containing rock, and then carrying out flame spraying treatment for 500s by using a flame spray gun, wherein the amount of the disodium ethylene diamine tetraacetate solution is 7% of the mass of the potassium-containing rock;
after spraying a disodium ethylene diamine tetraacetate solution with the concentration of 3% to the phosphogypsum, carrying out flame spraying treatment for 500s by using a flame spray gun, wherein the dosage of the disodium ethylene diamine tetraacetate solution is 7% of the mass of the phosphogypsum;
the preparation method of the cornstalk powder comprises the following steps: mixing corn stalks with water according to the weight ratio of 1: 3.5, performing frequency reduction ultrasonic treatment, drying in a low-temperature vacuum environment at the temperature of minus 5 ℃, and grinding to 600 meshes; the step of frequency reduction ultrasonic treatment is that the treatment is carried out for 50s under the condition that the frequency is 134kHz, the treatment is carried out for 135s at constant frequency by reducing the frequency to 40kHz at the speed of 0.5 kHz/min;
the magnetized water per liter contains 56mg of potassium, 81mg of phosphorus, 14mg of sulfur, 2mg of selenium, 0.03mg of europium and 2.5mg of allantoin;
the steam explosion treatment is to place the wheat straw with the granularity of 35 meshes in a steam explosion machine, wherein the explosion condition is 3.0Mpa, and the explosion time is 92 s.
Experimental example 1
The experiment is based on example 4, with the following differences between the comparative group and example 4:
comparative group 1: the bark is not soaked in a compound enzyme solution;
comparative group 2: the bark is not soaked in urea/silicon dioxide solution;
comparative group 3: ultrasonic waves are not utilized in the bark treatment;
comparative group 4: no ethylene diamine tetraacetic acid solution is sprayed in the treatment of manganese slag, potassium-containing rock and phosphogypsum;
comparative group 5: the corn stalk powder is not subjected to frequency reduction ultrasonic treatment;
comparative group 6: magnetized water is not added;
comparative group 7: the magnetized water does not contain allantoin;
comparative group 8: the magnetized water does not contain europium;
comparative group 9: step 1) adding no magnetized water;
comparative group 10: step 2), using no wheat straw;
the main measurement indexes comprise stem length, stem thickness, stem node number and single-stem fresh weight; fresh yield determination is carried out on all the fresh strips of the dendrobium candidum; then, deactivating enzyme at 105 ℃ for 10min, drying at 60 ℃ for 24h, measuring dry weight and calculating the dry folding rate; the results are shown in table 1:
TABLE 1
Measuring scoparone by a method of literature [ content measurement of scoparone in herba Dendrobii 4 plants ]; measuring coumarin content by RP-HPLC method, the results are shown in Table 2;
TABLE 2
Finally, it should be noted that the above embodiments are merely representative examples of the present invention. Obviously, the technical solution of the present invention is not limited to the above-described embodiments, and many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.
Claims (10)
1. A planting method of dendrobium pohuanense rich in scoparone is characterized by comprising the following steps:
1) and (3) treatment of the cultivation container: sequentially paving 8-12mm thick manganese slag, 10-14mm thick potassium-containing rock and 6-10mm thick phosphogypsum in a cultivation container, adding 50-70% of cornstalk powder by mass of the manganese slag into the cultivation container, irradiating for 30-45min under purple light, and finally adding 30-50% of magnetized water by mass of the cornstalk powder into the cultivation container;
2) cuttage: placing the nursery stock in a cultivation container, laying a layer of wheat straw with the thickness of 2-6mm after steam explosion treatment, and placing the cultivation container in a natural condition for planting.
2. The method for planting scoparone-enriched dendrobium candidum according to claim 1, wherein the pre-treatment of the bark is carried out by mixing the bark in a ratio of 1: (2.6-4.6) soaking the raw materials in a complex enzyme solution for 20-40min according to the mass ratio of the raw materials to the raw materials, and then mixing the raw materials according to the weight ratio of 1: (1.7-3.7) soaking in 30-50% sodium sulfide solution for 60-150min, and mixing with ultrasonic wave with frequency of 25-35kHz, taking out bark, and mixing the above materials at a ratio of 1: (1.4-2.1) soaking the material liquid in the urea/silicon dioxide composite solution at 35-45 ℃ for 45-75 min.
3. The method for planting the periwinkle-tree-shaped dendrobium nobile rich in scoparone as claimed in claim 2, wherein each ml of the complex enzyme solution contains 1900-.
4. The method for planting scoparone-enriched dendrobium candidum as claimed in claim 2, wherein the concentration of the urea/silica solution is 37-67%, and the mass ratio of urea to silica is 1: (0.3-0.9).
5. The method for planting the dendrobium candidum rich in scoparone as claimed in claim 1, wherein the granularity of the manganese ore slag, the potassium-containing rock and the phosphogypsum is 30-40 meshes.
6. The method for planting the dendrobii nobile lindl rich in scoparone as claimed in claim 1, wherein the manganese ore residue is sprayed with 1-5% disodium ethylene diamine tetraacetate solution, and then the solution is sprayed with flame spray gun for 300-800s, and the amount of the disodium ethylene diamine tetraacetate solution is 5-9% of the mass of the manganese ore residue.
7. The method as claimed in claim 1, wherein the potassium-containing rock is prepared by spraying 1-5% disodium EDTA solution onto the soil, and then spraying flame with a flame spray gun for 300-800s, wherein the amount of the disodium EDTA solution is 5-9% of the weight of the potassium-containing rock.
8. The method for planting the scoparone-rich dendrobium candidum as claimed in claim 1, wherein the phosphogypsum is sprayed with 1-5% disodium ethylenediamine tetraacetate solution, and then is treated with flame spraying for 300-800s, and the amount of the disodium ethylenediamine tetraacetate solution is 5-9% by mass of the phosphogypsum.
9. The method for planting scoparone-enriched dendrobium candidum according to claim 1, wherein the preparation method of the cornstalk powder comprises the following steps: mixing corn stalks with water according to the weight ratio of 1: (3.1-3.9), performing frequency reduction ultrasonic treatment, drying in a low-temperature vacuum environment at the temperature of-10-0 ℃, and grinding to 600 meshes; the step of the frequency reduction ultrasonic treatment is to treat the mixture for 35 to 60 seconds under the condition of the frequency of 126-142kHz, reduce the mixture to the frequency of 35 to 45kHz at the speed of 0.3 to 0.8kHz/min, and treat the mixture for 120-150 seconds at constant frequency.
10. The method for planting scoparone-enriched dendrobium candidum according to claim 1, wherein each liter of magnetized water contains 33-79mg of potassium, 54-107mg of phosphorus, 11-18mg of sulfur, 1-4mg of selenium, 0.01-0.04mg of europium and 1-4mg of allantoin.
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