CN110810227A - Propagation method for Lanzhou lily bulbs - Google Patents
Propagation method for Lanzhou lily bulbs Download PDFInfo
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- CN110810227A CN110810227A CN201911090151.XA CN201911090151A CN110810227A CN 110810227 A CN110810227 A CN 110810227A CN 201911090151 A CN201911090151 A CN 201911090151A CN 110810227 A CN110810227 A CN 110810227A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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Abstract
The invention discloses a Lanzhou lily seedball propagation method, which comprises the following steps: s1 selecting and processing seed balls; s2 scale treatment; s3 planting basket processing; s4 matrix treatment; s5, placing scales; s6 culturing at normal temperature; s7 hardening and culturing seedlings; s8 dormant culture of bulb. According to the invention, lily scales can be used as propagules to culture and obtain high-quality bulb bulbs with the diameter of 1-1.5 cm. By using the culture process, the probability of scale decay in the breeding process can be reduced, so that the success rate of seed ball breeding is improved; the seed balls cultured by the process have strong adaptability after being picked into a field, the survival rate is high, and the high-quality product rate of the mature lily is also high.
Description
Technical Field
The invention belongs to the technical field of lily planting, and particularly relates to a Lanzhou lily seedball propagation method.
Background
The Lanzhou lily is a perennial herb plant of Lilium of Liliaceae, one of four lily strains in China, has thick and rich scales and sweet taste, and has high edible and economic values. The Lanzhou lily has the sucrose content 2.6 times that of Yixing lily and 2.8 times that of Longya lily; the reducing sugar is 1.9 times of that of Yixing lily and Longya lily; the content of crude fiber is 17 percent less than that of Yixing lily and 23 percent less than that of Longya lily, and the sweet and delicious reputation and delicate texture of Lanzhou lily are realized because the Lanzhou lily has the highest sugar content and the least crude fiber content.
The propagation of lily includes sexual propagation, i.e. seed propagation, and asexual propagation, but the seedling has low rate of bearing, long period and low economic benefit. At present, vegetative propagation is mainly carried out through scale cuttage, but cuttage scales are easy to rot, and the scale propagation is sensitive to temperature, humidity, illumination and the like, so that the cuttage difficulty is high.
The conventional scale breeding method has the problems of slow growth speed and low breeding success rate when breeding Lanzhou lily at present.
Disclosure of Invention
The invention provides a propagation method of Lanzhou lily bulbs, and aims to solve the problems of low propagation efficiency and easy decay of scales in the conventional lily propagation process.
Therefore, the invention adopts the following technical means:
a Lanzhou lily seedball propagation method comprises the following steps:
s1: selecting and processing the seed balls: selecting a virus-free stock seed ball which is full in appearance, free of rot, disease and insect damage and free of damage; putting the mixture into a refrigeration house to be in forced dormancy for 10 to 20 days, controlling the temperature to be minus 2 to minus 5 ℃ at the front dormancy stage and controlling the temperature to be minus 6 to minus 10 ℃ at the rear dormancy stage; after the forced dormancy is finished, adjusting the temperature to 5-10 ℃ for dormancy transition, wherein the dormancy transition time is 3-5 days;
s2: scale treatment: taking out the seed balls after dormancy transition, peeling the scales layer by layer, selecting the scales with large sections and thick flesh as propagules, cleaning and disinfecting the selected scales, and then draining the scales by controlling water;
s3: planting basket treatment: cleaning and disinfecting the planting basket; cutting a plastic film, sterilizing the plastic film, and paving the sterilized plastic film on the basket bottom and the basket wall of the planting basket;
s4: matrix treatment: respectively sterilizing coconut chaff and humus at high temperature; then, soaking the coconut coir for decomposition to enable the water content to reach 10-20%; then, mixing coconut husk and humus according to the volume ratio of 1.5-2.5: 1 to obtain a mixed matrix;
s5: putting the scales: laying a mixed matrix with the thickness of 3-5 cm at the bottom of the planting basket, then placing the lily scales on the mixed matrix in a grid shape, wherein the interval between two adjacent lily scales is 3-6 cm, and then spreading the mixed matrix with the thickness of 3-5 cm on the lily scales; then placing lily scales, spreading the mixed matrix, placing 3-4 layers of lily scales in the above way, and finally covering a plastic film on the top of the planting basket;
s6: and (3) normal-temperature culture: placing the planting basket into a culture room, and controlling the temperature of the culture room to be 18-22 ℃; controlling the indoor air humidity to be 25-30% after the culture for 7-9 days;
on 9 th to 11 th days, a plurality of ventilation holes are formed in the plastic film on the periphery of the planting basket;
on day 14-16, filling oxygen into the planting basket through the ventilation holes for 3-10 min;
removing the plastic film on the top of the planting basket on 19-21 days, adjusting the relative humidity of air in the culture room to 40-65%, and differentiating the scales into bulb balls;
s7: hardening and culturing seedlings: taking the bulb balls out of the planting basket and putting the bulb balls into a seedling hardening chamber at 45-55 days; controlling the temperature to be 20-26 ℃, the illumination intensity to be 2000-2500 Lx, the illumination time to be 4-6 h every day, and culturing for 20-25 days to obtain a bulb with the diameter of 1-1.5 cm;
s8: and (3) bulb dormancy culture: and after the hardening is finished, putting the bulb balls into a planting basket filled with the mixed matrix, putting the planting basket into a refrigeration house for dormancy, controlling the temperature to be 0 +/-0.5 ℃, carrying out primary dormancy for 3-6 weeks, and putting the bulb balls into a refrigeration house with the temperature of-2 to-5 ℃ for refrigeration and strong dormancy.
Further, in step S6, after the planting basket is filled with oxygen on day 15, a fan is installed in the cultivation room to promote indoor air convection.
Further, the concentration of the oxygen gas charged in the step S6 is 20-30%.
Furthermore, the aperture of the ventilation holes in the step S6 is 3-4 cm, and the hole distance is 8-12 cm.
Further, the step of cleaning and disinfecting the scales in the step S2 is as follows: putting the scales into clear water at 10-25 ℃ to wash away silt, and then washing the scales with sterile water at 10-25 ℃ for 8-15 min; and then soaking the scales in a diluent of 40% difenoconazole for 1-3 min.
Further, the diluent of the 40% difenoconazole is as follows: 40% of difenoconazole and sterile water in a ratio of 3-8: dilution at a ratio of 100.
Further, the cleaning and disinfecting steps of the planting basket in the step S3 are as follows: washing the planting basket with clear water, and then putting the planting basket into the water tank 1: soaking in 800-1500 potassium permanganate diluent for 0.5-3.5 min.
Further, the sterilization step of the plastic film is as follows: and (3) soaking the plastic film in a diluent of 30% pyraclostrobin and sterile water for 8-15 min, wherein the dilution ratio is 10-25: 100.
Further, the plastic film was a 0.04mm polyethylene film.
The invention has the beneficial effects that: by using the culture process, the probability of scale decay in the breeding process can be reduced, so that the success rate of seed ball breeding is improved; the seed balls cultured by the process have strong adaptability after being picked into a field, the survival rate is high, and the high-quality product rate of the mature lily is also high.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.
Example 1:
a Lanzhou lily seedball propagation method comprises the following culture steps:
s1: selecting and processing the seed balls: selecting a virus-free stock seed ball which is full in appearance, free of rot, disease and insect damage and free of damage; placing into a refrigeration house for forced dormancy for 12 days, wherein the temperature is controlled to be-5 ℃ from day 1 to day 6, and the temperature is controlled to be-10 ℃ from day 7 to day 12; after dormancy, adjusting the temperature to 5 ℃ for dormancy transition, wherein the dormancy transition time is 5 days;
s2: scale treatment: then, peeling the scales of the seed balls layer by using a sharp blade, and selecting the middle-layer scales with large sections and thick flesh as propagules without damaging the base of the seed balls as much as possible. Because the scale on the outer layer is seriously damaged mechanically, has more germs and is easy to pollute; although the inner layer scale has strong cell regeneration capacity, the base part area is small, the nutrition accumulation is less, sufficient energy cannot be provided for differentiation, and the propagation coefficient is low, so the middle layer scale is the most ideal cuttage material.
Placing the selected scales into clear water at 15 ℃ to remove silt, and then washing with sterile water at 15 ℃ for 10 min; then the scales are transferred into a diluent of 40 percent of benzene ether methyl oxazole and sterile water with the ratio of 5:100 to be soaked for 2min, and the scales can be detoxified through the working procedure; taking out after soaking, placing in a shade place, draining.
S3: planting basket treatment: a plastic frame with meshes on the periphery is selected as a planting basket, and the length, width and height of the planting basket are 36cm multiplied by 26cm multiplied by 22 cm. Firstly, cleaning the planting basket with clear water, and then placing the planting basket into a container 1: soaking in 1000 potassium permanganate diluent for 1min, and air drying. Cutting 0.04mm thick polyethylene film to 40cm × 50cm, soaking the cut plastic film in 15:100 dilution of 30% pyraclostrobin and sterile water for 8 min; fishing out the plastic film and laying the plastic film on the basket bottom and the basket wall of the planting basket. The step is mainly used for sterilizing the planting basket and the plastic film and preventing bacteria and pathogens carried on the planting basket and the polyethylene from influencing the growth and propagation of the scale.
S4: matrix treatment: and (3) respectively carrying out high-temperature steam sterilization treatment on the coconut coir and the humus, wherein the steam sterilization is a method for introducing high-temperature steam (80-95 ℃) into the matrix to kill pathogenic bacteria. During disinfection, the coconut chaff and the humus are respectively placed in a special disinfection cabinet, steam is introduced through a high-temperature steam pipeline, and the sterilization cabinet is sealed for about 20-40 min, so that most of pathogenic bacteria and worm eggs can be killed. In steam sterilization, care is taken that the volume per sterilization is not excessive, otherwise it may cause the temperature of the inner portion of the substrate to fall short of the desired temperature. In addition, when steam sterilization is carried out, the substrate cannot be too moist or too dry, and the water content of the substrate is about 35 to 45 percent generally, so that the sterilization effect can be improved to the maximum extent. And after sterilization, soaking and decomposing the coconut coir to ensure that the water content of the coconut coir reaches 15%, and then mixing the coconut coir and humus according to the volume ratio of 2: 1 and mixing. The hand feeling judgment indexes are as follows: the substrate mixed by a small handle is held tightly by hands, water can not be extruded out but the substrate can form a dough shape, and the dough shape is slowly dispersed to a certain degree when the hands are loosened.
S5: putting the scales: laying a mixed matrix with the thickness of 3cm at the bottom of the planting basket, and placing the lily scales on the upper surface of the matrix in a latticed manner, wherein the distance between two adjacent lily scales is 6 cm. When placing, the flake concave parts face upwards and are sequentially placed on the substrate. Then continuously spreading a mixed matrix with the thickness of 3cm on the lily scales; placing the scales on the table, spreading the mixed matrix, and repeatedly placing 4 layers of lily scales. And finally, covering a plastic film on the top layer of the planting basket, and enabling the plastic film to be tightly attached to the substrate on the top of the planting basket. The scales have strong polarity, and the upward placement of the concave parts of the scales can not only effectively inhibit the upward growth potential energy, but also reduce the downward backflow potential energy of growth hormone at the top end to the base part, thereby reducing the influence on the bulb differentiation, so that the upward-placed increment coefficient of the inner sides of the scales is the highest.
S6: and (3) normal-temperature culture: placing the planting baskets in a culture room with an air conditioning and humidity adjusting system, wherein the distance between every two adjacent planting baskets is 10-15 cm; controlling the temperature of the culture room to be 22 ℃; adjusting the humidity in the culture chamber to 30% from the 8 th day of culture;
on the 11 th day, punching holes on the plastic film around the planting basket, wherein the hole diameter is 4cm, the hole distance is 12cm, and the holes are used for releasing excessive water in the film and preventing the bulbs from stopping differentiation due to overhigh water;
on day 16, oxygen with the concentration of 20 percent is filled into the planting basket through the holes on the plastic film by using an oxygen generator; during inflation, the periphery of the planting basket needs to be inflated, and the total inflation time is 10 min; meanwhile, the fan in the culture chamber is turned on to promote indoor air convection. Can promote the oxygen inside the planting basket and the air inside and outside the culture room to exchange air so as to increase the hedging speed of the air and the oxygen, thereby increasing the growth number of the planting points on the bulb sheet stem line, promoting the nitrogen fixation and the release of the decomposition amount of potassium of the propagation base material, and achieving the effects of rooting and promoting the growth.
Removing the plastic film on the top of the planting basket at the 20 th day, adjusting the relative humidity in the culture chamber to 40%, and differentiating bulblet bulbs on the stem lines of the scales after the plastic film is removed for 5-8 days, wherein 4-8 bulblet bulbs are differentiated in each slice;
s7: hardening and culturing seedlings: culturing at normal temperature for 45-55 days, taking out the bulb balls in the planting basket, and transferring the bulb balls into a seedling hardening chamber for hardening seedlings; controlling the temperature to be 20 ℃, the illumination intensity to be 2500lx, and the illumination time to be 5h every day, wherein the diameter of the bulb is increased to 1-1.5 cm after 20-25 days of culture;
s8: and (3) bulb dormancy culture: and after the hardening is finished, putting the bulbs into a planting basket filled with the mixed matrix, putting the bulbs into a cold storage for dormancy, controlling the temperature of the cold storage to be 0 +/-0.5 ℃, and putting the bulbs into the cold storage at the temperature of minus 2 ℃ to minus 5 ℃ for cold storage after the initial dormancy time is 5 weeks.
Example 2:
a Lanzhou lily seedball propagation method comprises the following steps:
s1: selecting and processing the seed balls: selecting a virus-free stock seed ball which is full in appearance, free of rot, disease and insect damage and free of damage; putting into a refrigeration house to be forced to sleep for 18 days, controlling the temperature to be-2 ℃ for 1 to 9 days, and controlling the temperature to be-6 ℃ for 10 to 18 days; after dormancy, adjusting the temperature to 10 ℃ for dormancy transition, wherein the dormancy transition time is 5 days;
s2: scale treatment: then taking out the seed balls, peeling the scales layer by layer, and selecting the scales with large sections and thick flesh as propagules; placing the selected scales into clear water at 25 ℃ to remove silt, and then washing with sterile water at 25 ℃ for 15 min; then putting the scales into a 5:100 solution of 40% difenoconazole and sterile water, soaking for 3min, then fishing out, draining;
s3: planting basket treatment: cleaning the planting basket with clear water, and then putting the planting basket into a container with the volume ratio of 1: soaking in 1000 potassium permanganate solution for 1.5 min; cutting a rectangular plastic film, and soaking the plastic film in 15:100 of 30% pyraclostrobin and sterile water solution for 15 min; fishing out the plastic film and laying the plastic film on the basket bottom and the basket wall of the planting basket;
s4: matrix treatment: respectively sterilizing coconut chaff and humus at high temperature; then, soaking the coconut coir for decomposition to ensure that the water content of the coconut coir reaches 20%; then, mixing coconut chaff and humus according to a volume ratio of 2: 1, mixing;
s5: putting the scales: laying a mixed matrix with the thickness of 5cm at the bottom of the planting basket, placing lily scales on the upper surface of the matrix in a grid shape, wherein the distance between two adjacent lily scales is 4cm, and continuously spreading the matrix for 5cm on the lily scales; then placing the lily scales and spreading a matrix, wherein after 3 layers of lily scales are placed in the manner, the planting basket is filled, and the top of the planting basket is covered with a plastic film;
s6: and (3) normal-temperature culture: placing the planting basket in a culture room, and controlling the temperature of the culture room to be 18 ℃; controlling the humidity to be 25% from the culture to the 7 th day;
on the 11 th day, punching holes on the plastic film around the planting basket for ventilation, wherein the hole diameter is 3cm, and the hole distance is 8 cm;
on day 15, filling oxygen with a concentration of 25% into the planting basket through the holes on the plastic film for 8 min; meanwhile, a fan is arranged in the culture room to promote indoor air convection;
on the 19 th day, removing the plastic film on the top of the planting basket, adjusting the relative humidity in the culture chamber to 50%, and differentiating bulblet bulbs on the stem lines of the scales 5-8 days after the plastic film is removed, wherein 4-8 bulblet bulbs are differentiated in each slice;
s7: hardening and culturing seedlings: taking out the bulbs with the bulb bulbs from the planting basket and putting the bulbs into a seedling hardening chamber at 45-55 days; controlling the temperature to be 26 ℃, the illumination intensity to be 2000lx, and the illumination time to be 5h every day, and culturing for 20-25 days to obtain a bulb with the diameter of 1-1.5 cm;
s8: and (3) bulb dormancy culture: putting the bulbs into a planting basket with a matrix after seedling hardening is finished, putting the bulbs into a refrigeration house for dormancy, controlling the temperature to be 0 +/-0.5 ℃, keeping the dormancy time for 4 weeks, and putting the bulbs into the refrigeration house with the temperature of minus 2-minus 5 ℃ for refrigeration.
Example 3:
a Lanzhou lily seedball propagation method comprises the following steps:
s1: selecting and processing the seed balls: selecting a virus-free stock seed ball which is full in appearance, free of rot, disease and insect damage and free of damage; putting into a refrigeration house to be forced to sleep for 15 days, controlling the temperature to be minus 3 ℃ for 1 to 7 days, and controlling the temperature to be minus 8 ℃ for 8 to 15 days; after dormancy, adjusting the temperature to 8 ℃ for dormancy transition, wherein the dormancy transition time is 5 days;
s2: scale treatment: then taking out the seed balls, peeling the scales layer by layer, and selecting the scales with large sections and thick flesh as propagules; placing the selected scales into clear water at 20 ℃ to remove silt, and then washing with sterile water at 20 ℃ for 15 min; then putting the scales into a 5:100 solution of 40% difenoconazole and sterile water, soaking for 3min, then fishing out, draining;
s3: planting basket treatment: cleaning the planting basket with clear water, and then putting the planting basket into a container with the volume ratio of 1: soaking in 1000 potassium permanganate solution for 1.5 min; cutting a rectangular plastic film, and soaking the plastic film in 15:100 of 30% pyraclostrobin and sterile water solution for 15 min; fishing out the plastic film and laying the plastic film on the basket bottom and the basket wall of the planting basket;
s4: matrix treatment: respectively sterilizing coconut chaff and humus at high temperature; then, soaking the coconut coir for decomposition to ensure that the water content of the coconut coir reaches 18 percent; then, mixing coconut chaff and humus according to a volume ratio of 2: 1, mixing;
s5: putting the scales: laying a mixed matrix with the thickness of 4cm at the bottom of the planting basket, placing the lily scales on the upper surface of the matrix in a grid shape, wherein the distance between two adjacent lily scales is 5cm, and continuously spreading the matrix for 4cm on the lily scales; then placing the lily scales and spreading a matrix, wherein after 4 layers of lily scales are placed in the manner, the planting basket is filled, and the top of the planting basket is covered with a plastic film;
s6: and (3) normal-temperature culture: placing the planting basket in a culture room, and controlling the temperature of the culture room to be 20 ℃; controlling the humidity to be 28% from the culture to the 7 th day;
on the 10 th day, punching holes on the plastic film around the planting basket for ventilation, wherein the hole diameter is 5cm, and the hole distance is 8 cm;
on the 14 th day, oxygen with the concentration of 28% is filled into the planting basket through the holes on the plastic film for 5 min; meanwhile, a fan is arranged in the culture room to promote indoor air convection;
on day 21, removing the plastic film on the top of the planting basket, adjusting the relative humidity in the culture chamber to 65%, and differentiating bulblet bulbs on the stem lines of the scales 5-8 days after the plastic film is removed, wherein 4-8 bulblet bulbs are differentiated in each slice;
s7: hardening and culturing seedlings: taking out the bulbs with the bulb bulbs from the planting basket and putting the bulbs into a seedling hardening chamber at 45-55 days; controlling the temperature to be 24 ℃, the illumination intensity to be 2200lx, and the illumination time to be 5h every day, and culturing for 20-25 days to obtain a bulb with the diameter of 1-1.5 cm;
s8: and (3) bulb dormancy culture: putting the bulbs into a planting basket with a matrix after seedling hardening is finished, putting the bulbs into a refrigeration house for dormancy, controlling the temperature to be 0 +/-0.5 ℃ and the dormancy time to be 6 weeks, and putting the bulbs into the refrigeration house with the temperature of minus 2-minus 5 ℃ for refrigeration.
It should be noted that the above are only some embodiments of the present invention, and it should be noted that, for those skilled in the art, many modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (9)
1. The propagation method of the Lanzhou lily seedballs is characterized by comprising the following steps:
s1: selecting and processing the seed balls: selecting a virus-free stock seed ball which is full in appearance, free of rot, disease and insect damage and free of damage; putting the mixture into a refrigeration house to be in forced dormancy for 10 to 20 days, controlling the temperature to be minus 2 to minus 5 ℃ at the front dormancy stage and controlling the temperature to be minus 6 to minus 10 ℃ at the rear dormancy stage; after the forced dormancy is finished, adjusting the temperature to 5-10 ℃ for dormancy transition, wherein the dormancy transition time is 3-5 days;
s2: scale treatment: taking out the seed balls after dormancy transition, peeling the scales layer by layer, selecting the scales with large sections and thick flesh as propagules, cleaning and disinfecting the selected scales, and then draining the scales by controlling water;
s3: planting basket treatment: cleaning and disinfecting the planting basket; cutting a plastic film, sterilizing the plastic film, and paving the sterilized plastic film on the basket bottom and the basket wall of the planting basket;
s4: matrix treatment: respectively sterilizing coconut chaff and humus at high temperature; then, soaking the coconut coir for decomposition to enable the water content to reach 10-20%; then, mixing coconut husk and humus according to the volume ratio of 1.5-2.5: 1 to obtain a mixed matrix;
s5: putting the scales: laying a mixed matrix with the thickness of 3-5 cm at the bottom of the planting basket, then placing the lily scales on the mixed matrix in a grid shape, wherein the interval between two adjacent lily scales is 3-6 cm, and then spreading the mixed matrix with the thickness of 3-5 cm on the lily scales; then placing lily scales, spreading the mixed matrix, placing 3-4 layers of lily scales in the above way, and finally covering a plastic film on the top of the planting basket;
s6: and (3) normal-temperature culture: placing the planting basket into a culture room, and controlling the temperature of the culture room to be 18-22 ℃; controlling the indoor air humidity to be 25-30% after the culture for 7-9 days;
on 9 th to 11 th days, a plurality of ventilation holes are formed in the plastic film on the periphery of the planting basket;
on day 14-16, filling oxygen into the planting basket through the ventilation holes for 3-10 min;
removing the plastic film on the top of the planting basket on 19-21 days, adjusting the relative humidity of air in the culture room to 40-65%, and differentiating the scales into bulb balls;
s7: hardening and culturing seedlings: taking the bulb balls out of the planting basket and putting the bulb balls into a seedling hardening chamber at 45-55 days; controlling the temperature to be 20-26 ℃, the illumination intensity to be 2000-2500 Lx, the illumination time to be 4-6 h every day, and culturing for 20-25 days to obtain a bulb with the diameter of 1-1.5 cm;
s8: and (3) bulb dormancy culture: and after the hardening is finished, putting the bulb balls into a planting basket filled with the mixed matrix, putting the planting basket into a refrigeration house for dormancy, controlling the temperature to be 0 +/-0.5 ℃, carrying out primary dormancy for 3-6 weeks, and putting the bulb balls into a refrigeration house with the temperature of-2 to-5 ℃ for refrigeration and strong dormancy.
2. The propagation method of lily bulbs of Lanzhou province as claimed in claim 1, wherein in step S6, after the planting basket is filled with oxygen on day 15, a fan is arranged in the cultivation room to promote indoor air convection.
3. The propagation method of the Lanzhou lily bulbs of claim 1 or 2, wherein the concentration of the oxygen charged in the step S6 is 20-30%.
4. The propagation expanding method for lily bulbs of Lanzhou province as claimed in claim 1, wherein the diameter of the ventilation holes in step S6 is 3-4 cm, and the distance between the ventilation holes is 8-12 cm.
5. The propagation method of lily bulbs of Lanzhou province as claimed in claim 1, wherein the step of scale washing and disinfection in step S2 is: putting the scales into clear water at 10-25 ℃ to wash away silt, and then washing the scales with sterile water at 10-25 ℃ for 8-15 min; and then soaking the scales in a diluent of 40% difenoconazole for 1-3 min.
6. The propagation expanding method for the Lanzhou lily bulbs of claim 5, wherein the diluent of 40% difenoconazole is: 40% of difenoconazole and sterile water in a ratio of 3-8: dilution at a ratio of 100.
7. The propagation method of lily bulbs of Lanzhou province as claimed in claim 1, wherein the cleaning and sterilizing steps of the planting baskets in step S3 are as follows: washing the planting basket with clear water, and then putting the planting basket into the water tank 1: soaking in 800-1500 potassium permanganate diluent for 0.5-3.5 min.
8. The propagation expanding method of the Lanzhou lily bulbs as claimed in claim 1, wherein the sterilization step of the plastic film is as follows: and (3) soaking the plastic film in a diluent of 30% pyraclostrobin and sterile water for 8-15 min, wherein the dilution ratio is 10-25: 100.
9. The propagation method of lily bulbs of Lanzhou province as claimed in claim 8, wherein the plastic film is a 0.04mm polyethylene film.
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