CN110760201A - Method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye - Google Patents
Method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye Download PDFInfo
- Publication number
- CN110760201A CN110760201A CN201911270306.8A CN201911270306A CN110760201A CN 110760201 A CN110760201 A CN 110760201A CN 201911270306 A CN201911270306 A CN 201911270306A CN 110760201 A CN110760201 A CN 110760201A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- isatis root
- dye
- blue
- leaves
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye, which comprises the following specific steps: taking materials: cutting fresh radix Isatidis stem and leaf; step two, fermentation; step three, precipitation: pumping out the upper water layer when the blue dye is precipitated under the liquid, adding humic acid and fermented fulvic acid into the upper water layer to obtain a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; step four, adopting a scientific and reasonable fermentation process, the invention improves the preparation rate of the isatis root vegetable dye, the dye uptake of the blue pigment can reach 40-55 percent, shortens the production time and has wide application range; the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method disclosed by the invention is wide in application, and the added value of the isatis root crops is improved after comprehensive utilization.
Description
Technical Field
The invention relates to a method for extracting and comprehensively utilizing isatis root vegetable dye by high-efficiency submerged fermentation.
Background
The radix isatidis is cool, is suitable for being planted in the north, and is particularly better in areas with large temperature difference between day and night. In the south of the yellow river, the temperature is high, so that the subculture reproduction rate of cabbage caterpillars is extremely high, the insect pests are several times of those in the north, and roots are easy to rot in rainy seasons, so that more cabbage caterpillars are planted in ancient areas, namely the north of the river, and ancient European areas. The weather in the contemporary era is rapidly warmed, so that the isatis tinctoria is planted more in the north of Liaoning, especially in Heilongjiang, Ningxia, Gansu and other places, China is the country with the largest planting amount of the isatis tinctoria in the world, and none of the countries is planted in the Zhangye area, and more than one million mu of the isatis tinctoria is planted in the Zhangye area. The major planting households in China are Chinese medicine factories such as Tongrentang and Baiyunshan, most of which are concentrated in Heilongjiang and adopt a professional GAP planting mode. Because a large amount of radix isatidis planting bases exist in China, radix isatidis is a Chinese herbal medicine encouraging planting in China, radix isatidis is used as a common Chinese herbal medicine, 2 times of leaf harvesting is required in the growing period, and the extract obtained after leaf fermentation is used as a blue dye. The root is still made of Chinese medicinal materials, so the root does not conflict with grains and Chinese medicine factories, and the method belongs to the high-efficiency comprehensive utilization of crops.
The isatis root (isatis tinctoria) is different from the sophora japonica, the Indigofera tinctoria and the Polygonum tinctoria, and the blue pigment in the mature fresh leaves is not in a form of being combined with glycosidic bonds to form glycosidic bonds, but directly exists in the fresh leaves in an indole form, so that the indole does not need to be separated by cutting off the glycosidic bonds through long-time fermentation. The fermentation time of isatis root (isatis tinctoria) leaves is the shortest for all blue plants, even if the fermentation time is the first fermentation time, the fermentation time can not exceed one day, and the subsequent inoculation fermentation can greatly shorten the fermentation time, so the isatis tinctoria (isatis tinctoria) leaves are most suitable for large-batch industrial production, but the biological fermentation of isatis tinctoria fresh stems and leaves for preparing plant dyes is not adopted in China, and the isatis tinctoria fresh stems and leaves are not used for other purposes, so the existing purposes are single.
Disclosure of Invention
The invention aims to overcome the defects of the existing product and provides a method for efficiently fermenting, extracting and comprehensively utilizing the isatis root vegetable dye under liquid.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
taking materials: cutting fresh radix Isatidis stem and leaf;
step two, fermentation: weighing 5-10 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh isatis root stem leaves below the liquid level by using bamboo chips, then fermenting at the temperature of 20-28 ℃, wherein the fermentation time is set according to different temperatures, the fermentation time is not more than 26 hours, stopping fermentation when the leaf main vein and the leaf stalk are soft and discolor, fishing out all the stem leaves, drying and crushing the fished stem leaves to obtain stem leaf powder, and the stem leaf powder is used for a natural Chinese herbal medicine feed additive;
step three, precipitation: adding oyster shell powder and food-grade lime into a fermentation tank with all stems and leaves fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for a period of time, extracting upper-layer water when the blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper-layer water to obtain a liquid fertilizer, wherein the liquid fertilizer is used for planting soil;
step four, obtaining the blue pasty vegetable dye: taking out the lower layer of the blue pasty plant dye from the fermentation tank, and sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing mill.
Preferably, the length of the stem and leaf of the isatis root is 2 cm.
Preferably, in the first step, fresh stems and leaves of radix Isatidis are harvested in the morning when dew is present or the sun is absent.
Preferably, the time consumed for cutting the fresh isatis root stems and leaves on the ground is 4 hours.
Preferably, the fermentation time is set according to the temperature: if the temperature is more than or equal to 20 ℃ and less than 25 ℃, the fermentation time is 24-26 hours; if the temperature is more than or equal to 25 ℃ and less than 28 ℃, the fermentation time is 22-24 hours; if the temperature is equal to 28 ℃, the fermentation time is 20-22 hours.
Preferably, the period of time is 24 hours or more.
Preferably, in the second step, the fermentation tank is sealed and insulated for preventing the fermentation tank from being exposed to the sun and contacting the air.
Preferably, the weight of the oyster shell powder is 1.5 to 3 percent of the weight of the fresh isatis root stems and leaves.
The invention has the following beneficial effects: the invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
Detailed Description
The technical solution of the present invention is further explained below:
example 1:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
taking materials: cutting off the stem and leaf of radix Isatidis in the morning when dew is present or sun is absent, and harvesting in 4 hr with the length of 2 cm;
step two, fermentation: weighing 5-10 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh isatis root stem leaves below the liquid surface by using bamboo chips, and then fermenting at the temperature of 20-28 ℃, wherein the fermentation time is set according to different temperatures, and if the temperature is more than or equal to 20 ℃ and less than 25 ℃, the fermentation time is 24-26 hours; if the temperature is more than or equal to 25 ℃ and less than 28 ℃, the fermentation time is 22-24 hours; if the temperature is equal to 28 ℃, the fermentation time is 20-22 hours, the fermentation time is not more than 26 hours, the fermentation is stopped when the leaf veins and the leaf stalks are soft and discolor, all the stems and leaves are fished out, and the fished stems and leaves are dried and crushed to obtain stem and leaf powder which is used as a natural Chinese herbal medicine feed additive; the fermentation tank needs to be sealed and insulated for preventing sunshine and air contact.
Step three, precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for more than 24 hours, extracting upper water when blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper water, and obtaining a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; the weight of the oyster shell powder is 1.5 to 3 percent of that of the fresh isatis root stem leaves.
Step four, obtaining the blue pasty vegetable dye: taking out the lower layer of the blue pasty plant dye from the fermentation tank, and sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing mill.
The invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
Example 2:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
the method comprises the following steps: selecting the isatis roots planted between 38 and 48 degrees in the latitude of north latitude, and in the mature period of leaves, the method is characterized in that: the damaged part of the leaf has deep concentrated juice and emits the special ammonia fragrance of the isatis root, when the leaf is grabbed by hands and has crisp sound, fresh isatis root stems and leaves 2 cm above the ground are cut when dew exists in the morning or the sun does not appear, the collection is finished within 4 hours, and the collected fresh isatis root stems and leaves are used for preparing the isatis root vegetable dye.
Step two: weighing 5-10 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh bamboo chip isatis root stem leaves below the liquid level, fermenting for 26 hours at 20 ℃, wherein the fermentation tank needs to be sealed and insulated, particularly prevents sunshine and air from contacting, and all the stem leaves are fished out when the main veins and the petioles of the leaves are soft and change color. Sun drying the stem and leaf, and pulverizing to obtain stem and leaf powder used as natural Chinese medicinal feed additive.
Step three: and (3) precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for more than 24 hours, extracting upper-layer water when blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper-layer water, and obtaining a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; the weight of oyster shell powder is 2% of the weight of fresh isatis root stems and leaves, standing is carried out for more than 24 hours, upper-layer water is pumped out when blue dye is precipitated under liquid, humic acid and fermented fulvic acid are added into the upper-layer water, and then liquid fertilizer is obtained and is used for planting soil;
step four: obtaining blue pasty vegetable dye: taking out the lower layer of blue pasty plant dye from the fermentation tank, sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing factory, the preservation period of the blue pasty plant dye isolated from air is more than 3 years, and the pasty plant dye can be directly used for plant dyeing in the dyeing factory.
The invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
Example 3:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
the method comprises the following steps: selecting the isatis roots planted between 38 and 48 degrees in the latitude of north latitude, and in the mature period of leaves, the method is characterized in that: the damaged part of the leaf has deep concentrated juice and emits the special ammonia fragrance of the isatis root, when the leaf is grabbed by hands and has crisp sound, fresh isatis root stems and leaves 2 cm above the ground are cut when dew exists in the morning or the sun does not appear, the collection is finished within 4 hours, and the collected fresh isatis root stems and leaves are used for preparing the isatis root vegetable dye.
Step two: weighing 5-10 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh bamboo chip isatis root stem leaves below the liquid level, fermenting for 23 hours at 25 ℃, wherein the fermentation tank needs to be sealed and insulated, particularly prevents sunshine and air from contacting, and all the stem leaves are fished out when the main veins and the petioles of the leaves are soft and change color. Sun drying the stem and leaf, and pulverizing to obtain stem and leaf powder used as natural Chinese medicinal feed additive.
Step three: and (3) precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for more than 24 hours, extracting upper-layer water when blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper-layer water to obtain a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; the weight of oyster shell powder is 2.2% of the weight of fresh isatis root stems and leaves, standing is carried out for more than 24 hours, when blue dye is precipitated under liquid, upper-layer water is pumped out, humic acid and fermented fulvic acid are added into the upper-layer water to obtain a liquid fertilizer, and the liquid fertilizer is used for planting soil;
step four: obtaining blue pasty vegetable dye: taking out the lower layer of blue pasty plant dye from the fermentation tank, sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing factory, the preservation period of the blue pasty plant dye isolated from air is more than 3 years, and the pasty plant dye can be directly used for plant dyeing in the dyeing factory.
The invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
Example 4:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
the method comprises the following steps: selecting the isatis roots planted between 38 and 48 degrees in the latitude of north latitude, and in the mature period of leaves, the method is characterized in that: the damaged part of the leaf has deep concentrated juice and emits the special ammonia fragrance of the isatis root, when the leaf is grabbed by hands and has crisp sound, fresh isatis root stems and leaves 2 cm above the ground are cut when dew exists in the morning or the sun does not appear, the collection is finished within 4 hours, and the collected fresh isatis root stems and leaves are used for preparing the isatis root vegetable dye.
Step two: weighing 5-10 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh bamboo chip isatis root stem leaves below the liquid level, fermenting for 20 hours at 28 ℃, wherein the fermentation tank needs to be sealed and insulated, particularly prevents sunshine and air from contacting, and all the stem leaves are fished out when the main veins and the petioles of the leaves are soft and change color. Sun drying the stem and leaf, and pulverizing to obtain stem and leaf powder used as natural Chinese medicinal feed additive.
Step three: and (3) precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for more than 24 hours, extracting upper-layer water when blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper-layer water to obtain a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; the weight of oyster shell powder is 2.5 percent of that of fresh isatis root stems and leaves, the mixture is kept still for more than 24 hours, upper-layer water is pumped out when blue dye is precipitated under liquid, the upper-layer water is added with humic acid and fermented fulvic acid to obtain liquid fertilizer, and the liquid fertilizer is used for planting soil;
step four: obtaining blue pasty vegetable dye: taking out the lower layer of blue pasty plant dye from the fermentation tank, sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing factory, the storage life of the blue pasty plant dye in an air-isolated state is more than 3 years, the pasty plant dye can be directly used for plant dyeing in the dyeing factory, and the dye uptake of the blue pasty plant dye can reach more than 50%.
The invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
Example 5:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
taking materials: cutting off the stem and leaf of radix Isatidis in the morning when dew is present or sun is absent, and harvesting in 4 hr with the length of 2 cm;
step two, fermentation: weighing 5 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh isatis root stem leaves below the liquid level by using bamboo chips, and then fermenting at the temperature of 20-28 ℃, wherein the fermentation time is set according to different temperatures, and if the temperature is more than or equal to 20 ℃ and less than 25 ℃, the fermentation time is 24-26 hours; if the temperature is more than or equal to 25 ℃ and less than 28 ℃, the fermentation time is 22-24 hours; if the temperature is equal to 28 ℃, the fermentation time is 20-22 hours, the fermentation time is not more than 26 hours, the fermentation is stopped when the leaf veins and the leaf stalks are soft and discolor, all the stems and leaves are fished out, and the fished stems and leaves are dried and crushed to obtain stem and leaf powder which is used as a natural Chinese herbal medicine feed additive; the fermentation tank needs to be sealed and insulated for preventing sunshine and air contact.
Step three, precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for more than 24 hours, extracting upper water when blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper water, and obtaining a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; the weight of the oyster shell powder is 1.5 percent of that of the fresh isatis root stem leaves.
Step four, obtaining the blue pasty vegetable dye: taking out the lower layer of the blue pasty plant dye from the fermentation tank, and sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing mill.
The invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
Example 6:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
taking materials: cutting off the stem and leaf of radix Isatidis in the morning when dew is present or sun is absent, and harvesting in 4 hr with the length of 2 cm;
step two, fermentation: weighing 10 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh isatis root stem leaves below the liquid level by using bamboo chips, and then fermenting at the temperature of 20-28 ℃, wherein the fermentation time is set according to different temperatures, and if the temperature is more than or equal to 20 ℃ and less than 25 ℃, the fermentation time is 24-26 hours; if the temperature is more than or equal to 25 ℃ and less than 28 ℃, the fermentation time is 22-24 hours; if the temperature is equal to 28 ℃, the fermentation time is 20-22 hours, the fermentation time is not more than 26 hours, the fermentation is stopped when the leaf veins and the leaf stalks are soft and discolor, all the stems and leaves are fished out, and the fished stems and leaves are dried and crushed to obtain stem and leaf powder which is used as a natural Chinese herbal medicine feed additive; the fermentation tank needs to be sealed and insulated for preventing sunshine and air contact.
Step three, precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for more than 24 hours, extracting upper water when blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper water, and obtaining a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; the weight of the oyster shell powder is 3 percent of that of the fresh isatis root stem leaves.
Step four, obtaining the blue pasty vegetable dye: taking out the lower layer of the blue pasty plant dye from the fermentation tank, and sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing mill.
The invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
Example 7:
the method for extracting and comprehensively utilizing the isatis root vegetable dye by high-efficiency submerged fermentation comprises the following specific steps:
taking materials: cutting off the stem and leaf of radix Isatidis in the morning when dew is present or sun is absent, and harvesting in 4 hr with the length of 2 cm;
step two, fermentation: weighing 8 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh isatis root stem leaves below the liquid level by using bamboo chips, and then fermenting at the temperature of 20-28 ℃, wherein the fermentation time is set according to different temperatures, and if the temperature is more than or equal to 20 ℃ and less than 25 ℃, the fermentation time is 24-26 hours; if the temperature is more than or equal to 25 ℃ and less than 28 ℃, the fermentation time is 22-24 hours; if the temperature is equal to 28 ℃, the fermentation time is 20-22 hours, the fermentation time is not more than 26 hours, the fermentation is stopped when the leaf veins and the leaf stalks are soft and discolor, all the stems and leaves are fished out, and the fished stems and leaves are dried and crushed to obtain stem and leaf powder which is used as a natural Chinese herbal medicine feed additive; the fermentation tank needs to be sealed and insulated for preventing sunshine and air contact.
Step three, precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for more than 24 hours, extracting upper water when blue dye is precipitated under liquid, adding humic acid and fermented fulvic acid into the upper water, and obtaining a liquid fertilizer, wherein the liquid fertilizer is used for planting soil; the weight of the oyster shell powder is 1.8 percent of that of the fresh isatis root stem leaves.
Step four, obtaining the blue pasty vegetable dye: taking out the lower layer of the blue pasty plant dye from the fermentation tank, and sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing mill.
The invention makes the stems and leaves of the Chinese herbal medicine radix isatidis widely planted in China fully and comprehensively utilized, improves the planting yield and makes the radix isatidis as a high-value economic crop; by adopting a scientific and reasonable fermentation process, the preparation rate of the isatis root vegetable dye is improved, the dye uptake of the blue pigment can reach 40-55%, the production time is shortened, the method can be applied to large-batch industrial production, the fermentation time is properly prolonged, the method is also suitable for extracting the blue vegetable dye such as the polygonum blue, the kale and the like, and the application range is wide; the residual stem leaves after the plant dye is extracted are dried and crushed to obtain stem leaf powder, the stem leaf powder is used as a natural Chinese herbal medicine feed additive, the extracted upper layer water is added with humic acid and fermented fulvic acid to obtain a liquid fertilizer, the liquid fertilizer is used for planting soil, and the blue paste plant dye is directly used for dyeing plants in a dye house, so that the fermentation method has wide application, and the added value of the isatis root crops is improved after comprehensive utilization.
It should be noted that the above list is only one specific embodiment of the present invention. It is clear that the invention is not limited to the embodiments described above, but that many variations are possible, all of which can be derived or suggested directly from the disclosure of the invention by a person skilled in the art, and are considered to be within the scope of the invention.
Claims (8)
1. The method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye is characterized by comprising the following specific steps of:
taking materials: cutting fresh radix Isatidis stem and leaf;
step two, fermentation: weighing 5-10 parts of clear water and 1 part of fresh isatis root stem leaves according to the weight parts, pouring the clear water and the fresh isatis root stem leaves into a fermentation tank, pressing the fresh isatis root stem leaves below the liquid level by using bamboo chips, then fermenting at the temperature of 20-28 ℃, wherein the fermentation time is set according to different temperatures, the fermentation time is not more than 26 hours, stopping fermentation when the leaf main vein and the leaf stalk are soft and discolor, fishing out all the stem leaves, drying and crushing the fished stem leaves to obtain stem leaf powder, and the stem leaf powder is used for a natural Chinese herbal medicine feed additive;
step three, precipitation: adding oyster shell powder and food-grade lime into a fermentation tank after all stems and leaves are fished out, filling compressed air into the fermentation tank, continuously stirring at a high speed until the solution is blue-purple, standing for a period of time, pumping out upper-layer water when blue dye is precipitated under liquid, and adding humic acid and fermented fulvic acid into the upper-layer water to obtain a liquid fertilizer, wherein the liquid fertilizer is used for planting soil;
step four, obtaining the blue pasty vegetable dye: taking out the lower layer of the blue pasty plant dye from the fermentation tank, and sealing the blue pasty plant dye in a storage vat, wherein the blue pasty plant dye is directly used for plant dyeing in a dyeing mill.
2. The method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye according to claim 1, wherein the length of the stem and leaf of isatis root is 2 cm.
3. The method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye according to claim 1, wherein in the first step, fresh isatis root stems and leaves on the ground are cut off when dew exists in the morning or the sun does not emerge.
4. The method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye according to claim 3, wherein the time consumed for harvesting fresh isatis root stems and leaves on the ground is 4 hours.
5. The method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye according to claim 1, wherein the fermentation time is set according to different temperatures as follows: if the temperature is more than or equal to 20 ℃ and less than 25 ℃, the fermentation time is 24-26 hours; if the temperature is more than or equal to 25 ℃ and less than 28 ℃, the fermentation time is 22-24 hours; if the temperature is equal to 28 ℃, the fermentation time is 20-22 hours.
6. The method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye according to claim 1, wherein the period of time is more than 24 hours.
7. The method for efficient submerged fermentation, extraction and comprehensive utilization of isatis root vegetable dyes as claimed in claim 1, wherein in the second step, the fermentation tank is sealed and insulated for preventing sun exposure and air contact.
8. The method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye according to claim 1, wherein the weight of the oyster shell powder is 1.5% -3% of the weight of fresh isatis root stems and leaves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911270306.8A CN110760201B (en) | 2019-12-12 | 2019-12-12 | Method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911270306.8A CN110760201B (en) | 2019-12-12 | 2019-12-12 | Method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110760201A true CN110760201A (en) | 2020-02-07 |
| CN110760201B CN110760201B (en) | 2021-06-29 |
Family
ID=69341624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911270306.8A Active CN110760201B (en) | 2019-12-12 | 2019-12-12 | Method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110760201B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111287000A (en) * | 2020-04-01 | 2020-06-16 | 杭州彩润科技有限公司 | Method for dyeing normal-pressure dyeable modified polyester fibers and fabrics by using blue plant dye |
| CN111500087A (en) * | 2020-04-24 | 2020-08-07 | 杭州彩润科技有限公司 | Method for preparing natural Maya blue by utilizing isatis roots through pre-reduction method |
| CN117064930A (en) * | 2023-08-21 | 2023-11-17 | 山东迅达康兽药有限公司 | Isatis tinctoria fresh root extract, preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107337935A (en) * | 2017-06-30 | 2017-11-10 | 李斌 | A kind of Preparation method and use of madder red pigment |
| CN107365512A (en) * | 2016-05-12 | 2017-11-21 | 雷炳忠 | From a kind of method of the indigo natural dye of Radix Isatidis plant extract |
| CN108864736A (en) * | 2018-06-29 | 2018-11-23 | 中国农业科学院油料作物研究所 | The method of melanin and its application of method sediment obtained are extracted in a kind of rapeseed meal |
| CN109674843A (en) * | 2019-01-30 | 2019-04-26 | 湖南绿蔓生物科技股份有限公司 | A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization |
| CN110105121A (en) * | 2019-04-16 | 2019-08-09 | 广西鼎好农业科技有限公司 | A kind of sargassum method of comprehensive utilization |
-
2019
- 2019-12-12 CN CN201911270306.8A patent/CN110760201B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365512A (en) * | 2016-05-12 | 2017-11-21 | 雷炳忠 | From a kind of method of the indigo natural dye of Radix Isatidis plant extract |
| CN107337935A (en) * | 2017-06-30 | 2017-11-10 | 李斌 | A kind of Preparation method and use of madder red pigment |
| CN108864736A (en) * | 2018-06-29 | 2018-11-23 | 中国农业科学院油料作物研究所 | The method of melanin and its application of method sediment obtained are extracted in a kind of rapeseed meal |
| CN109674843A (en) * | 2019-01-30 | 2019-04-26 | 湖南绿蔓生物科技股份有限公司 | A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization |
| CN110105121A (en) * | 2019-04-16 | 2019-08-09 | 广西鼎好农业科技有限公司 | A kind of sargassum method of comprehensive utilization |
Non-Patent Citations (1)
| Title |
|---|
| 苗艳丽等: "牡蛎壳粉用于板蓝根水提液的吸附澄清研究", 《中成药》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111287000A (en) * | 2020-04-01 | 2020-06-16 | 杭州彩润科技有限公司 | Method for dyeing normal-pressure dyeable modified polyester fibers and fabrics by using blue plant dye |
| CN111287000B (en) * | 2020-04-01 | 2022-05-03 | 杭州彩润科技有限公司 | Method for dyeing normal-pressure dyeable modified polyester fibers and fabrics by using blue plant dye |
| CN111500087A (en) * | 2020-04-24 | 2020-08-07 | 杭州彩润科技有限公司 | Method for preparing natural Maya blue by utilizing isatis roots through pre-reduction method |
| CN117064930A (en) * | 2023-08-21 | 2023-11-17 | 山东迅达康兽药有限公司 | Isatis tinctoria fresh root extract, preparation method and application thereof |
| CN117064930B (en) * | 2023-08-21 | 2024-05-03 | 山东迅达康兽药有限公司 | Isatis tinctoria fresh root extract, preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110760201B (en) | 2021-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104303967B (en) | Implantation methods on the tree of a kind of Huoshan Herba Dendrobii | |
| CN103125243B (en) | Method for raising seedlings of camellia chrysantha plants by top grafting | |
| CN110760201B (en) | Method for efficient submerged fermentation extraction and comprehensive utilization of isatis root vegetable dye | |
| CN104604498A (en) | Purple heart pitaya planting and cultivating method | |
| CN105724011B (en) | A kind of plantation breeding method of selenium-rich tree peony | |
| CN105724010B (en) | A kind of selenium-rich oil implantation methods of tree peony | |
| CN105613015B (en) | Planting method of selenium-rich peony | |
| CN105052452A (en) | Intercropping method of osmanthusfragrans trees, tea trees and ginkgo trees | |
| CN106386131A (en) | Dragon fruit planting method | |
| CN105123242A (en) | Ecological wintering method for planting passion fruit in temperate area | |
| CN104957016A (en) | Preparation method of culture medium of dendrobium officinale | |
| CN1176571C (en) | Cultivation of new variety of santengzhumiguo fruit and its natural colouring matter extraction method | |
| CN109122032A (en) | A kind of implantation methods of tea tree interplanting white beech mushroom | |
| CN107298595A (en) | A kind of pure natural ecological fertilizer planted for umbellate pore furgus and preparation method thereof | |
| CN106258329A (en) | A kind of cultural method of red jujube tree catch cropping alfalfa | |
| CN102754681B (en) | Storing and fresh-keeping method of fresh radix puerariae | |
| CN105524187A (en) | Preparation method of Talinum paniculatum polysaccharides and beta-sitosterol | |
| CN105294338A (en) | Zenia insigins Chun. special fertilizer and production method thereof | |
| CN106588406A (en) | Method of utilizing waste woven bags to plant sweet potatoes high efficiently | |
| CN106258781A (en) | A kind of grape cultivation method | |
| CN109105155A (en) | A kind of implantation methods of tea tree interplanting needle mushroom | |
| JP3962585B2 (en) | Stevia extract manufacturing method | |
| CN108323399A (en) | A kind of implantation methods of Jasmine | |
| CN110476712B (en) | Method for cultivating agrocybe cylindracea by using stems and leaves of curcuma kwangsiensis | |
| CN109122034A (en) | A kind of implantation methods of tea tree interplanting phoenix-tail mushroom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |