CN110736654A - 一种腮腺炎病毒大鼠神经毒力评价模型 - Google Patents
一种腮腺炎病毒大鼠神经毒力评价模型 Download PDFInfo
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- CN110736654A CN110736654A CN201911065583.5A CN201911065583A CN110736654A CN 110736654 A CN110736654 A CN 110736654A CN 201911065583 A CN201911065583 A CN 201911065583A CN 110736654 A CN110736654 A CN 110736654A
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Abstract
Description
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Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911065583.5A CN110736654B (zh) | 2019-11-04 | 2019-11-04 | 一种腮腺炎病毒大鼠神经毒力评价模型 |
US17/755,664 US20220404359A1 (en) | 2019-11-04 | 2020-11-03 | Device for evaluating neurovirulence of mumps virus |
EP20885871.2A EP4056983A4 (en) | 2019-11-04 | 2020-11-03 | MUMPS VIRUS NEUROVIRULENCE ASSESSMENT DEVICE |
PCT/CN2020/126249 WO2021088826A1 (zh) | 2019-11-04 | 2020-11-03 | 一种用于评估腮腺炎病毒神经毒力的装置 |
Applications Claiming Priority (1)
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CN201911065583.5A CN110736654B (zh) | 2019-11-04 | 2019-11-04 | 一种腮腺炎病毒大鼠神经毒力评价模型 |
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CN110736654A true CN110736654A (zh) | 2020-01-31 |
CN110736654B CN110736654B (zh) | 2021-11-19 |
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CN201911065583.5A Active CN110736654B (zh) | 2019-11-04 | 2019-11-04 | 一种腮腺炎病毒大鼠神经毒力评价模型 |
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US (1) | US20220404359A1 (zh) |
EP (1) | EP4056983A4 (zh) |
CN (1) | CN110736654B (zh) |
WO (1) | WO2021088826A1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021088826A1 (zh) * | 2019-11-04 | 2021-05-14 | 上海青赛生物科技有限公司 | 一种用于评估腮腺炎病毒神经毒力的装置 |
CN114794018A (zh) * | 2022-06-28 | 2022-07-29 | 北京赛尔富森生物科技有限公司 | 一种腮腺炎病毒攻毒动物模型及其建立方法 |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1517009A (zh) * | 2003-01-10 | 2004-08-04 | 格罗瓦克斯有限公司 | 用于制备各种生物制品的无病原体特异仓鼠的培育方法 |
US20080170989A1 (en) * | 2007-01-11 | 2008-07-17 | Immunomedics, Inc. | Methods and Compositions for Improved F-18 Labeling of Proteins, Peptides and Other Molecules |
JP2013024587A (ja) * | 2011-07-15 | 2013-02-04 | Clear Office Kk | 抗菌加工品の抗菌性能の評価方法 |
CN103808655A (zh) * | 2014-02-21 | 2014-05-21 | 中南大学湘雅医院 | 一种研究高压氧对脑出血大鼠脑内血管新生影响的方法 |
CN104031879A (zh) * | 2014-05-21 | 2014-09-10 | 北京农学院 | 一种体外分离并培养大鼠脑微血管内皮细胞的方法 |
CN106645153A (zh) * | 2016-12-23 | 2017-05-10 | 麦克奥迪(厦门)医疗诊断系统有限公司 | 一种细胞病理切片快速染色检测方法 |
CN108956242A (zh) * | 2018-05-16 | 2018-12-07 | 绍兴文理学院 | 一种乳腺癌腋窝淋巴结离体组织标本的固定处理方法 |
CN109030431A (zh) * | 2018-06-04 | 2018-12-18 | 华中科技大学苏州脑空间信息研究院 | 一种利用水溶性光吸收剂提升图像信噪比的方法 |
US20190086317A1 (en) * | 2017-09-20 | 2019-03-21 | Sysmex Corporation | Cell analysis method, cell information providing apparatus, cell information providing system |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2163229C (en) * | 1994-03-18 | 2000-03-14 | Shinobu Abe | Method for examining neurovirulence of polio virus |
CA2535034A1 (en) * | 2003-06-27 | 2005-01-27 | Renomedix Institute Inc | Internallly administered therapeutic agents for diseases in central and peripheral nervous system comprising mesenchymal cells as an active ingredient |
CA2975491A1 (en) * | 2015-02-17 | 2016-08-25 | Terrance P. Snutch | Methods of treating brain edema |
CN106023291B (zh) * | 2016-05-12 | 2019-05-17 | 华中科技大学 | 快速获取大样本三维结构信息和分子表型信息的成像装置和方法 |
CN106226509B (zh) * | 2016-08-12 | 2019-09-06 | 中国人民解放军第四军医大学 | 一种在小鼠前扣带回皮层诱发dse现象的方法 |
CN110736654B (zh) * | 2019-11-04 | 2021-11-19 | 上海青赛生物科技有限公司 | 一种腮腺炎病毒大鼠神经毒力评价模型 |
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2019
- 2019-11-04 CN CN201911065583.5A patent/CN110736654B/zh active Active
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2020
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- 2020-11-03 US US17/755,664 patent/US20220404359A1/en active Pending
- 2020-11-03 EP EP20885871.2A patent/EP4056983A4/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1517009A (zh) * | 2003-01-10 | 2004-08-04 | 格罗瓦克斯有限公司 | 用于制备各种生物制品的无病原体特异仓鼠的培育方法 |
US20080170989A1 (en) * | 2007-01-11 | 2008-07-17 | Immunomedics, Inc. | Methods and Compositions for Improved F-18 Labeling of Proteins, Peptides and Other Molecules |
JP2013024587A (ja) * | 2011-07-15 | 2013-02-04 | Clear Office Kk | 抗菌加工品の抗菌性能の評価方法 |
CN103808655A (zh) * | 2014-02-21 | 2014-05-21 | 中南大学湘雅医院 | 一种研究高压氧对脑出血大鼠脑内血管新生影响的方法 |
CN104031879A (zh) * | 2014-05-21 | 2014-09-10 | 北京农学院 | 一种体外分离并培养大鼠脑微血管内皮细胞的方法 |
CN106645153A (zh) * | 2016-12-23 | 2017-05-10 | 麦克奥迪(厦门)医疗诊断系统有限公司 | 一种细胞病理切片快速染色检测方法 |
US20190086317A1 (en) * | 2017-09-20 | 2019-03-21 | Sysmex Corporation | Cell analysis method, cell information providing apparatus, cell information providing system |
CN108956242A (zh) * | 2018-05-16 | 2018-12-07 | 绍兴文理学院 | 一种乳腺癌腋窝淋巴结离体组织标本的固定处理方法 |
CN109030431A (zh) * | 2018-06-04 | 2018-12-18 | 华中科技大学苏州脑空间信息研究院 | 一种利用水溶性光吸收剂提升图像信噪比的方法 |
Non-Patent Citations (1)
Title |
---|
STEVEN A.RUBIN 等: ""Evaluation of a Neonatal Rat Model for Prediction of Mumps Virus Neurovirulence in Humans"", 《JOURNAL OF VIROLOGY》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021088826A1 (zh) * | 2019-11-04 | 2021-05-14 | 上海青赛生物科技有限公司 | 一种用于评估腮腺炎病毒神经毒力的装置 |
CN114794018A (zh) * | 2022-06-28 | 2022-07-29 | 北京赛尔富森生物科技有限公司 | 一种腮腺炎病毒攻毒动物模型及其建立方法 |
CN114794018B (zh) * | 2022-06-28 | 2022-11-04 | 北京赛尔富森生物科技有限公司 | 一种腮腺炎病毒攻毒动物模型及其建立方法 |
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