CN110734994A8 - 用于检测嗜水气单胞菌的特异性引物对、探针和检测试剂盒 - Google Patents

用于检测嗜水气单胞菌的特异性引物对、探针和检测试剂盒 Download PDF

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CN110734994A8
CN110734994A8 CN201911255363.9A CN201911255363A CN110734994A8 CN 110734994 A8 CN110734994 A8 CN 110734994A8 CN 201911255363 A CN201911255363 A CN 201911255363A CN 110734994 A8 CN110734994 A8 CN 110734994A8
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detection
aeromonas hydrophila
primer pair
specific primer
probe
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CN201911255363.9A
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CN110734994B (zh
CN110734994A (zh
Inventor
吴亚锋
刘肖汉
王楠楠
王晶晶
刘训猛
陈静
袁锐
于继彬
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Haimen Aquatic Technology Guidance Station
Jiangsu Fishery Technology Promotion Center
Jinling Institute of Technology
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Haimen Aquatic Technology Guidance Station
Jiangsu Fishery Technology Promotion Center
Jinling Institute of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
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  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明公开了一种用于检测嗜水气单胞菌的特异性引物对以及所述的引物对配合使用的探针。本发明还公开了一种检测试剂盒。本发明以嗜水气单胞菌中aerA基因为检测靶点，通过恒温扩增技术，使用特异性的引物和探针组合，提高了嗜水气单胞菌的检测便捷性和特异性，同时检测时间也大大缩短。与PCR检测方法相比，本发明的方法省去了产物电泳验证过程，避免了假阳性结果出现，提高检测的准确度。与qPCR相比，本发明的方法简便易行，也不需要操作复杂的仪器设备，节约了成本，提高了检测效率，同时便于在大范围内推广使用。与其他恒温扩增方法相比，本发明的检测方法所需时间更短，检测的准确率更高。
CN201911255363.9A 2019-12-10 2019-12-10 用于检测嗜水气单胞菌的特异性引物对、探针和检测试剂盒 Active CN110734994B (zh)

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CN111793702A (zh) * 2020-06-16 2020-10-20 中国水产科学研究院珠江水产研究所 一种用于检测嗜水气单胞菌的rpa引物组、探针和试剂盒及其应用
CN111850144A (zh) * 2020-07-08 2020-10-30 南开大学 一种对亲水气单胞菌血清型o抗原分子分型的检测方法及应用
CN111850145A (zh) * 2020-07-08 2020-10-30 南开大学 一种对亲水气单胞菌o7、o16、o19、o24等血清型o抗原分子分型的检测方法
CN114540513A (zh) * 2021-12-31 2022-05-27 江苏海洋大学 一种rpa-lfs快速检测嗜水气单胞菌的引物和探针组合物及方法

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IE81145B1 (en) * 1989-04-20 2000-05-03 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences
CN1978665A (zh) * 2005-12-02 2007-06-13 中国水产科学研究院珠江水产研究所 水生实验动物剑尾鱼病原菌－嗜水气单胞菌pcr检测试剂盒及检测方法
CN105018466A (zh) * 2015-07-17 2015-11-04 浙江泰晶生物科技有限公司 一种荧光探针法常温等温核酸扩增法

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CI02 Correction of invention patent application

Correction item: Applicant|Address|Applicant

Correct: Jiangsu fishery technology extension center| 210000 No. 302, Hanzhongmen street, Gulou District, Nanjing City, Jiangsu Province|Jinling University of science and technology; Haimen aquatic technology guidance station

False: Jiangsu fishery technology extension center|210000 No. 302, Hanzhongmen street, Gulou District, Nanjing City, Jiangsu Province| Jinling University of science and technology; Haimen aquatic technology guidance station

Number: 05-01

Page: The title page

Volume: 36

Correction item: Applicant|Address|Applicant

Correct: Jiangsu fishery technology extension center| 210000 No. 302, Hanzhongmen street, Gulou District, Nanjing City, Jiangsu Province|Jinling University of science and technology; Haimen aquatic technology guidance station

False: Jiangsu fishery technology extension center| 210000 No. 302, Hanzhongmen street, Gulou District, Nanjing City, Jiangsu Province|Jinling University of science and technology; Haimen aquatic technology guidance station

Number: 05-01

Volume: 36

CI02 Correction of invention patent application
GR01 Patent grant
GR01 Patent grant