CN110664694A

CN110664694A - Plant-extracted non-irritant itching-relieving repair liquid for skin

Info

Publication number: CN110664694A
Application number: CN201911110775.3A
Authority: CN
Inventors: 吴明超; 于群亮
Original assignee: Shenzhen Meige Tianhua Biotechnology Co Ltd
Current assignee: Shenzhen Meige Tianhua Biotechnology Co Ltd
Priority date: 2019-11-14
Filing date: 2019-11-14
Publication date: 2020-01-10

Abstract

The invention relates to the technical field of itching relieving liquid, and provides a plant-extracted non-irritant itching relieving repair liquid for skin, which comprises the following raw materials in percentage by weight: 0.04-20% of plant extract, 0.02-0.4% of polypeptide substance, 0.02-20% of lysozyme and the balance of water. The skin care product does not use any chemical reagent such as preservative, is natural, safe, environment-friendly and non-irritant, can quickly relieve itching, repair, inhibit bacteria and diminish inflammation, has no any irritation to eyes, skin and the like, can be applied to skin of infants, and provides healthy protection for the skin of the infants.

Description

Plant-extracted non-irritant itching-relieving repair liquid for skin

Technical Field

The invention relates to the technical field of itching relieving liquid, in particular to a plant-extracted non-irritant itching relieving repair liquid for skin, a preparation method and application thereof.

Background

The skin is an important component for maintaining the life and health of human body, is the first barrier of the body, is directly contacted with the outside, and is easily damaged by the influence of environmental factors. In recent years, the skin is prone to itch due to air pollution, abuse of various industrial additives, changes in life habits of people, and the like. Itching is an unpleasant sensation distinguished from pain and causes a desire for scratching the body, and is not only a common symptom of clinical various skin diseases but also a concomitant symptom of systemic diseases. In daily life, itching can occur due to mosquito bites, allergen contact, drug administration and the like. The pruritus is not cured for a long time and is mostly accompanied by symptoms of depression, insomnia, anxiety and the like. It can be said that pruritus is a common disease, which not only seriously affects people's daily life, but also seriously affects people's quality of life.

In the prior art, the repair effect is not strong while relieving itching; and a plurality of the skin care products are added with preservatives, have certain irritation and have certain potential safety hazard on the skin of the infants. The invention aims to develop a safe and nonirritating liquid, which has the functions of quickly relieving itching, repairing and inhibiting bacteria on the skin of infants and children and provides the healthy protection for the skin of infants and children.

Disclosure of Invention

In order to solve the technical problems, the invention provides a plant-extracted non-irritating itching-relieving repair liquid for skin, which comprises the following raw materials in percentage by weight: 0.04-20% of plant extract, 0.02-0.4% of polypeptide substance, 0.02-20% of lysozyme and the balance of water.

As a preferred technical scheme, the plant extract non-irritant itching-relieving repair liquid for skin comprises the following raw materials in percentage by weight: 5-15% of plant extract, 0.1-0.2% of polypeptide substance, 8-15% of lysozyme and the balance of water.

As a preferred technical solution, the plant extract in the present invention is selected from one or more of a chamomile extract, a centella asiatica extract, and a mint extract.

As a preferred technical solution, the weight ratio of the golden chamomile extract, the centella asiatica extract and the mint extract in the invention is 1: (0.5-2.5): (0.05-0.5).

As a preferred technical solution, the method for extracting a chamomile extract in the present invention comprises the following steps: (1) crushing golden chamomile to 200-mesh and 300-mesh, adding 60-85 wt% ethanol solution, and soaking for 15-24 h; (2) adding hydrochloric acid with the concentration of 5-10 mol% into the step (1) to adjust the pH value to 4-6, carrying out ultrasonic treatment for 1-2h, and filtering to obtain the compound.

As a preferable technical scheme, the mass ratio of the golden chamomile to the ethanol solution in the invention is 1: (5-15).

As a preferable technical scheme, the extraction power of the ultrasound is 800-.

As a preferred technical scheme, the polypeptide substance is tripeptide-1 copper.

The second aspect of the invention provides a preparation method of the plant extraction non-irritant itching-relieving repair liquid for skin,

the preparation method comprises the following steps: sequentially adding the plant extract, the polypeptide substances and the lysozyme into water, stirring and mixing uniformly, and filtering with 100-mesh and 300-mesh filter cloth.

The third aspect of the invention provides application of the plant extract non-irritant itching-relieving repair liquid for skin, which can be applied to skin of infants and children.

Compared with the prior art, the invention has the following beneficial effects:

the plant-extracted non-irritant itching-relieving repair liquid for the skin is natural, safe, environment-friendly and non-irritant, does not use any chemical reagent such as a preservative and the like, can quickly relieve itching, repair, inhibit bacteria and diminish inflammation, does not have any irritant effect on eyes, skin and the like, can be applied to the skin of infants and children, and provides the healthy protection for the skin of the infants and children.

Detailed Description

The technical features of the technical solutions provided by the present invention are further clearly and completely described below with reference to the specific embodiments, and the scope of protection is not limited thereto.

The words "preferred", "more preferred", and the like, in the present invention refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.

When a range of values is disclosed herein, the range is considered to be continuous and includes both the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values of the range is included. Further, when multiple range-describing features or characteristics are provided, the ranges may be combined. In other words, unless otherwise indicated, all ranges disclosed herein are to be understood to encompass any and all subranges subsumed therein. For example, a stated range from "1 to 10" should be considered to include any and all subranges between the minimum value of 1 and the maximum value of 10. Exemplary subranges of the range 1 to 10 include, but are not limited to, 1 to 6.1, 3.5 to 7.8, 5.5 to 10, and the like.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.

According to the first aspect of the invention, the plant-extracted non-irritant itching-relieving repair liquid for skin comprises the following raw materials in percentage by weight: 0.04-20% of plant extract, 0.02-0.4% of polypeptide substance, 0.02-20% of lysozyme and the balance of water.

In some preferred embodiments, the plant extract for skin is a non-irritant itching-relieving repair liquid, and comprises the following raw materials in percentage by weight: 5-15% of plant extract, 0.1-0.2% of polypeptide substance, 8-15% of lysozyme and the balance of water.

In some more preferred embodiments, the plant for skin extraction is a non-irritating antipruritic liquid, and comprises the following raw materials in percentage by weight: 10% of plant extract, 0.15% of polypeptide substance, 12% of lysozyme and the balance of water.

Plant extracts

The plant extract is a product formed by taking plants as raw materials, extracting and separating the raw materials by a physical or chemical method according to the requirements of final products, and directionally obtaining and concentrating one or more active ingredients in the plants without changing the effective structures of the active ingredients. The effective components of the composition are different from each other due to different parts, including roots, stems, leaves, peels, flowers, fruits, corolla, seeds, sprouts and the like, and the composition is widely applied to skin medicines, curative cosmetics, cosmetics and the like.

In some embodiments, the plant extract is selected from the group consisting of a combination of one or more of a chamomile extract, a centella asiatica extract, and a mint extract; preferably, the plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract.

In some embodiments, the weight ratio of the golden chamomile extract, the centella asiatica extract, and the mint extract is 1: (0.5-2.5): (0.05-0.5); preferably, the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: (1-2): (0.1-0.3); more preferably, the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: 1.5: 0.2.

chrysanthemum indicum extract

The golden chamomile is also called Roman chamomile, the feverfew is a plant of Compositae, the original product is Europe, the height is about 30 centimeters, the center is yellow, the petals are white, and the leaves are slightly hairy antler. The golden yellow chamomile extract has the effects of helping sleep, relieving itching, resisting bacteria and diminishing inflammation, relieving inflammation and pain symptoms of a patient and relieving insomnia caused by nervous skin itch.

In some embodiments, the method for extracting a chamomile extract comprises the following steps: (1) crushing golden chamomile to 200-mesh and 300-mesh, adding 60-85 wt% ethanol solution, and soaking for 15-24 h; (2) adding hydrochloric acid with the concentration of 5-10 mol% into the step (1) to adjust the pH value to 4-6, carrying out ultrasonic treatment for 1-2h, and filtering to obtain the compound.

In some preferred embodiments, the method for extracting the golden chamomile extract comprises the following steps: (1) pulverizing dried flos Matricariae Chamomillae to 300 mesh, adding 75 wt% ethanol solution, and soaking for 20 hr; (2) adding hydrochloric acid with the concentration of 8 mol% into the step (1) to adjust the pH value to 4, carrying out ultrasonic treatment for 1.5h, and filtering to obtain the product.

In some embodiments, the mass ratio of the golden chamomile to the ethanol solution is 1: (5-15); preferably, the mass ratio of the golden chamomile to the ethanol solution is 1: 10.

in some embodiments, the extraction power of the ultrasound in step (2) is 800-; preferably, the extraction power of the ultrasound is 1000W.

Centella asiatica extract

The centella asiatica is an Umbelliferae perennial herb, and has creeping, slender and rooting stems. The lamina membranacea is in the shape of grass, round, kidney or horseshoe; the palmate pulse has two raised sides and the upper part is bifurcated; the leaf stalk is 1.5-27 cm long, has no hair or soft hair on the upper part, and has transparent basal leaf sheath and membranous texture. 2-4 umbelliform inflorescence stalks gather in leaf axils; each umbelliform inflorescence has 3-4 pieces of flowers, and the aggregation is head-shaped; the petals are oval, purple red or milky white, membranous; the length of the flower column is about 0.6 mm; the filaments are shorter than the petals and are as long as the flower columns. The fruit is flat pressed on two sides, is spherical, has a base part in a shape from heart to frustum, has a plurality of longitudinal edges on each side, has obvious small transverse veins among the edges, is reticular, and has hair or smooth surface. The flower and fruit period is 4-10 months. The whole herb is used as a medicine for clearing heat and promoting diuresis, reducing swelling and detoxifying, and treating acute respiratory syndrome, abdominal pain, summer diarrhea, dysentery, damp-heat jaundice, stranguria with sand, bloody stranguria, hematemesis, hemoptysis, conjunctival congestion, throat swelling, rubella, mange, furuncle, carbuncle, toxic swelling, traumatic injury and the like. The herba Centellae extract has antioxidant, antiinflammatory, antibacterial, and wound healing effects.

Examples centella asiatica extracts, manufacturers: shanghai Xinkai medicine science and technology Limited, purity 99%, CAS number: 84696-21-9.

Mint extract

The mint is a perennial herbaceous plant, the stem is upright, a plurality of sections of slender fibrous roots and horizontal creeping rhizomes are arranged at the lower part of the mint, the mint is in a sharp four-burrow shape and has four grooves, the upper part of the mint is inverted to micro-soft hair, and the mint is only slightly soft hair and multi-branched along the burrows. The flowering period is 6-9 months, the fruit period is 10 months, the plants grow in humid areas beside water, and the altitude can reach 3500 m. It has strong adaptability to temperature, and its rhizome can survive and live through winter, and can resist low temperature of-15 deg.c. The growth of the fertilizer is optimally at 25-30 ℃, the growth is slow when the temperature is lower than 15 ℃, and the growth is accelerated when the temperature is higher than 20 ℃. At the temperature of 20-30 ℃, as long as the water and fertilizer are appropriate, the higher the temperature is, the faster the growth is; the nature is favorable to sunlight for long-day crops. The sun is long, the blooming of the mint can be promoted, and the accumulation of the mint oil and the menthol is facilitated; the requirements on the soil are not very strict, and the soil can be planted in general except for sandy loam and alluvial soil with excessive viscosity, excessive pH value and poor low-lying drainage, preferably sandy loam and alluvial soil, and the pH value of the soil is preferably 6-7.5. The mint extract is mainly used for treating: disperse wind-heat, clear head and eyes, promote eruption, and can be used for treating wind-heat type common cold, early stage of wind-heat type, headache, conjunctival congestion, pharyngitis, aphtha, rubella, measles, and chest and hypochondrium swelling and distress.

The golden chamomile extract can relieve itching, bacteria and inflammation, relieve inflammation and pain symptoms of patients, and the centella asiatica extract also has the effects of oxidation resistance, inflammation resistance, bacteria resistance, wound healing and the like. In the experimental process, the inventor does not use any chemical reagent such as preservative, is natural, safe, environment-friendly and non-irritant, can quickly relieve itching, repair, inhibit bacteria and diminish inflammation, does not have any irritant effect on eyes, skin and the like, can be applied to the skin of infants and children, and provides the healthy protection for the skin of the infants and children.

However, the inventors found that, in the present invention, a chamomile extract and a centella asiatica extract are used as main components, and the prepared itching relieving repair liquid has storage stability without adding a preservative, and the itching relieving efficacy also gradually becomes low, and particularly after 3 months, a flocculated precipitate occurs, and the stability is poor. The inventor unexpectedly finds that the mint extract with a specific dosage is added into the system, so that the skin capillary vessel is dilated, the effects of inhibiting simple scar and rash viruses, forest viruses, staphylococcus aureus, staphylococcus albus and the like are achieved, and the effects of enhancing the skin ventilation, absorption and itching relieving are achieved; the inventor finds that the storage stability of the itching relieving repair liquid can be effectively improved and the itching relieving effect can be improved after the mint extract is added, probably because the mint extract contains more menthol, menthone, menthol, mint and other components, and can generate physical action supporting force with more components with different electronegativities, such as madecassoside molecules and proteins, in the golden chamomile extract and the centella extract, so that attraction between molecular structures with opposite electronegativities is prevented, and the formation of flocculation precipitates is prevented. However, for infants and children, the mint extract can generate certain irritation, and the inventor finds that the golden chamomile extract, the mint extract and the centella asiatica extract which are extracted after hydrochloric acid with certain concentration is added in the extraction process are compounded with one another for use, so that the irritation problem of the mint extract can be solved, and the mint extract has very effective effects of repairing skin, relieving itching and diminishing inflammation.

In the examples, the mint extract, manufacturer: guangzhou Yuxuan Hemerocallis Biotech Co., Ltd.

Polypeptides

The polypeptide substance refers to a compound formed by connecting alpha-amino acids together by peptide bonds, and a compound formed by dehydrating and condensing two amino acid molecules is called dipeptide, and similarly, tripeptide, tetrapeptide, pentapeptide and the like are also included. Generally, a compound obtained by dehydration condensation of 10 to 100 amino acid molecules is called a polypeptide.

In one embodiment, the polypeptide is the tripeptide-1 copper.

The tripeptide-1 copper is also known as copper triside peptide and copper bluepeptide. The copper peptide and the copper compound thereof can be used as an activator and an antioxidant for tissue remodeling, and the copper peptide is also a signal peptide, so that the degradation of a large number of collagen aggregates outside the scar, the synthesis of normal collagen of the skin, the generation of elastin, proteoglycan and glucosaminan, the growth speed and migration of different cell types, inflammation resistance and antioxidant reaction are promoted. The polypeptide substance is compounded with the plant extract, so that the itching relieving, repairing and bacteriostasis are fast achieved, and simultaneously, the safety and the nonirritant are realized.

Example tripeptide-1 copper, manufacturer: doudu Yunshi chemical Co., Ltd., brand: yunshi, CAS number: 49557-75-7.

Lysozyme

The lysozyme is also called muramidase (muramidase) or N-acetylmuramidase (N-acetylmuramidase glycohydrolase), and is an alkaline enzyme capable of hydrolyzing mucopolysaccharide in pathogenic bacteria. The bacterial lysis is achieved by breaking the beta-1, 4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, breaking down the cell wall insoluble mucopolysaccharide into soluble glycopeptides, causing the contents of the broken cell wall to escape. Lysozyme can also be directly combined with virus protein with negative charge, and forms double salt with DNA, RNA and apoprotein to inactivate virus. Therefore, the enzyme has the functions of antibiosis, antiphlogosis, antivirus, etc.

Lysozyme in the examples, the manufacturer: zhengzhou yu-control biotechnology limited company, brand: space control and enzyme activity preservation rate: 98%, CAS number: 12650-88-3.

The second aspect of the invention provides a preparation method of the plant-extracted non-irritant itching-relieving repair liquid for skin, which comprises the following preparation steps: sequentially adding the plant extract, the polypeptide substances and the lysozyme into water, stirring and mixing uniformly, and filtering with 100-mesh and 300-mesh filter cloth.

In some preferred embodiments, the preparation method of the plant extract non-irritant itching-relieving repair liquid for skin comprises the following steps: sequentially adding the plant extract, the polypeptide substances and the lysozyme into water, stirring and mixing uniformly, and filtering with 250-mesh filter cloth.

The term baby refers to a male or female with an age of 1-8 years.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention. In addition, the starting materials used are all commercially available, unless otherwise specified.

Example 1

A plant-extracted non-irritating itching-relieving repair liquid for skin comprises the following raw materials in percentage by weight: 10% of plant extract, 0.15% of polypeptide substance, 12% of lysozyme and the balance of water.

The plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract; the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: 1.5: 0.2. the extraction method of the golden yellow chamomile extract comprises the following steps: (1) pulverizing dried flos Matricariae Chamomillae to 300 mesh, adding 75 wt% ethanol solution, and soaking for 20 hr; (2) adding hydrochloric acid with the concentration of 8 mol% into the step (1) to adjust the pH value to 4, carrying out ultrasonic treatment for 1.5h, and filtering to obtain the product. The mass ratio of the golden chamomile to the ethanol solution is 1: 10. the extraction power of the ultrasound was 1000W. The centella asiatica extract is purchased from Shanghai Xinkai pharmaceutical science and technology Limited, with a purity of 99%. The mint extract was obtained from Hemerocallis guangzhou Biotech limited. The polypeptide substance is tripeptide-1 copper.

The preparation method of the plant extraction non-irritant itching-relieving repair liquid for skin comprises the following preparation steps: sequentially adding the plant extract, the polypeptide substances and the lysozyme into water, stirring and mixing uniformly, and filtering with 250-mesh filter cloth.

Example 2

A plant-extracted non-irritating itching-relieving repair liquid for skin comprises the following raw materials in percentage by weight: 5% of plant extract, 0.1% of polypeptide substance, 8% of lysozyme and the balance of water.

The plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract; the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: 0.5: 0.05. the extraction method of the golden yellow chamomile extract comprises the following steps: (1) crushing dried golden yellow chamomile to 200 meshes, adding an ethanol solution with the concentration of 60 wt%, and soaking for 24 hours; (2) adding hydrochloric acid with the concentration of 5 mol% into the step (1) to adjust the pH value to 6, carrying out ultrasonic treatment for 2h, and filtering to obtain the product. The mass ratio of the golden chamomile to the ethanol solution is 1: 5. the extraction power of the ultrasound was 800W. The centella asiatica extract is purchased from Shanghai Xinkai pharmaceutical science and technology Limited, with a purity of 99%. The mint extract was obtained from Hemerocallis guangzhou Biotech limited. The polypeptide substance is tripeptide-1 copper.

The preparation method of the plant extraction non-irritant itching-relieving repair liquid for skin comprises the following preparation steps: sequentially adding plant extract, polypeptide substances, and lysozyme into water, stirring, mixing, and filtering with 100 mesh filter cloth.

Example 3

A plant-extracted non-irritating itching-relieving repair liquid for skin comprises the following raw materials in percentage by weight: 15% of plant extract, 0.2% of polypeptide substance, 15% of lysozyme and the balance of water.

The plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract; the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: 2.5: 0.5. the extraction method of the golden yellow chamomile extract comprises the following steps: (1) crushing dried golden yellow chamomile to 300 meshes, adding an ethanol solution with the concentration of 85 wt%, and soaking for 15 h; (2) adding hydrochloric acid with the concentration of 10 mol% into the step (1) to adjust the pH value to 4, carrying out ultrasonic treatment for 1h, and filtering to obtain the product. The mass ratio of the golden chamomile to the ethanol solution is 1: 15. the extraction power of the ultrasound was 1200W. The centella asiatica extract is purchased from Shanghai Xinkai pharmaceutical science and technology Limited, with a purity of 99%. The mint extract was obtained from Hemerocallis guangzhou Biotech limited. The polypeptide substance is tripeptide-1 copper.

The preparation method of the plant extraction non-irritant itching-relieving repair liquid for skin comprises the following preparation steps: sequentially adding plant extract, polypeptide substances, and lysozyme into water, stirring, mixing, and filtering with 300 mesh filter cloth.

Example 4

Example 5

The plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract; the weight ratio of the golden yellow chamomile extract to the centella asiatica extract is 1: 1.5. the extraction method of the golden yellow chamomile extract comprises the following steps: (1) pulverizing dried flos Matricariae Chamomillae to 300 mesh, adding 75 wt% ethanol solution, and soaking for 20 hr; (2) adding hydrochloric acid with the concentration of 8 mol% into the step (1) to adjust the pH value to 4, carrying out ultrasonic treatment for 1.5h, and filtering to obtain the product. The mass ratio of the golden chamomile to the ethanol solution is 1: 10. the extraction power of the ultrasound was 1000W. The centella asiatica extract is purchased from Shanghai Xinkai pharmaceutical science and technology Limited, with a purity of 99%. The polypeptide substance is tripeptide-1 copper.

Example 6

The plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract; the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: 1.5: 0.01. the extraction method of the golden yellow chamomile extract comprises the following steps: (1) pulverizing dried flos Matricariae Chamomillae to 300 mesh, adding 75 wt% ethanol solution, and soaking for 20 hr; (2) adding hydrochloric acid with the concentration of 8 mol% into the step (1) to adjust the pH value to 4, carrying out ultrasonic treatment for 1.5h, and filtering to obtain the product. The mass ratio of the golden chamomile to the ethanol solution is 1: 10. the extraction power of the ultrasound was 1000W. The centella asiatica extract is purchased from Shanghai Xinkai pharmaceutical science and technology Limited, with a purity of 99%. The mint extract was obtained from Hemerocallis guangzhou Biotech limited. The polypeptide substance is tripeptide-1 copper.

Example 7

The plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract; the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: 1.5: 0.2. the extraction method of the golden yellow chamomile extract comprises the following steps: (1) pulverizing dried flos Matricariae Chamomillae to 300 mesh, adding 75 wt% ethanol solution, soaking for 20 hr, performing ultrasonic treatment for 1.5 hr, and filtering. The mass ratio of the golden chamomile to the ethanol solution is 1: 10. the extraction power of the ultrasound was 1000W. The centella asiatica extract is purchased from Shanghai Xinkai pharmaceutical science and technology Limited, with a purity of 99%. The mint extract was obtained from Hemerocallis guangzhou Biotech limited. The polypeptide substance is tripeptide-1 copper.

Example 8

The plant extract is selected from the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract; the weight ratio of the golden chamomile extract to the centella asiatica extract to the mint extract is 1: 4: 0.2. the extraction method of the golden yellow chamomile extract comprises the following steps: (1) pulverizing dried flos Matricariae Chamomillae to 300 mesh, adding 75 wt% ethanol solution, and soaking for 20 hr; (2) adding hydrochloric acid with the concentration of 8 mol% into the step (1) to adjust the pH value to 4, carrying out ultrasonic treatment for 1.5h, and filtering to obtain the product. The mass ratio of the golden chamomile to the ethanol solution is 1: 10. the extraction power of the ultrasound was 1000W. The centella asiatica extract is purchased from Shanghai Xinkai pharmaceutical science and technology Limited, with a purity of 99%. The mint extract was obtained from Hemerocallis guangzhou Biotech limited. The polypeptide substance is tripeptide-1 copper.

Performance testing

First, stability test

The plant-extracted non-irritating antipruritic liquid for skin prepared in examples 1 to 8 was used as a subject to be tested for stability.

The test method comprises the following steps: the experimental subject is placed in an environment of 40 ℃ in a closed manner, after three months, whether the flocculation precipitation phenomenon exists or not is observed, and the experimental result is shown in the following table:

results of Table stability test

Examples	Stability test results	Examples	Stability test results
				Example 1	No flocculation precipitation	Example 5	Has flocculation and precipitation
Example 2	Has flocculation and precipitation	Example 6	Has flocculation and precipitation
				Example 3	No flocculation precipitation	Example 7	No flocculation precipitation
Example 4	No flocculation precipitation	Example 8	Has flocculation and precipitation

II, toxicology tests are as follows:

the plant-extracted non-irritating itching-relieving repair liquid for skin prepared in example 1 was used as an experimental subject to perform toxicology tests and safety tests:

acute eye irritation test

1. The test substance: a light yellow transparent liquid was used as it was for the test.

2. Animal and feeding environment: selecting common-grade, adult and healthy white New Zealand rabbits, 3 male rabbits, with the weight of more than 1.7 Kg; provided by animal farms of the family of: SCXK (yue) 2017-; feeding animals with conventional feed, and the feed source is as follows: guangdong province medical experiment animal center, the production license number: guangdong feeding (2019)05073, freely drinking city domestic water, raising in a common animal laboratory in a single cage, temperature: 20 ℃ to 25 ℃, relative humidity: 40% -70%, 12h illumination and 12h darkness; license for use of experimental animal: SYXK (Yue) 2016-.

3. The test method comprises the following steps: the rabbit was acclimatized for 3 d. 2% fluorescein sodium solution is dripped into eyes of rabbits 24 hours before formal test, and the eyes are inspected by a handheld slit lamp after being washed, so that the test paper has no irritation symptom, corneal defect and conjunctival injury. During the test, 0.1ml of the test object is taken and dripped into the conjunctival sac of the left eye of the rabbit, the upper eyelid and the lower eyelid are passively closed for 1s, the eye on the other side is not treated and used as self control, and the eye is not washed within 24h after the test object is dripped. After the test substance is dripped, the eyes of the rabbits are inspected by using a handheld slit lamp for 1h and 24h respectively. After 24h observation and recording, all rabbits were further examined by instillation of 2% sodium fluorescein solution in their eyes. The eye irritation intensity of the test substance was judged according to "eye irritation response grading" of table 3 product according to "eye irritation response grading" of table 3, based on the highest integral mean value and recovery time of the irritation response of the rabbit cornea, iris or conjunctiva at 24h, 48h or 72h observation time, respectively, by scoring according to "eye irritation/corrosivity test of table 1" evaluation criteria for eye injury "of technical Specification for safety of cosmetics (2015 edition).

And (3) test results:

experimental result of acute eye irritation of test object to rabbit

The conjunctiva, cornea and iris scores of the animals were all 0 at each observation time of 24h, 48h and 72h, and no other toxic symptoms were observed. The test result of the acute eye irritation of the test object to the rabbit is nonirritant.

Multiple skin irritation tests:

2. Animal and feeding environment: selecting common-grade, adult, healthy and skin-intact white New Zealand rabbits, 4 rabbits, males and more than 1.7Kg of body weight; the method is provided by animal farms in the family of the Longguixing in the white cloud area of Guangzhou city, and the production license of experimental animals is as follows: SCXK (yue) 2017-; feeding animals with conventional feed, and the feed source is as follows: guangdong province medical experiment animal center, the production license number: guangdong feeding (2019)05073, freely drinking city domestic water, raising in a common animal laboratory in a single cage, temperature: 20 ℃ to 25 ℃, relative humidity: 40% -70%, 12h illumination and 12h darkness; license for use of experimental animal: SYXK (Yue) 2016-.

3. The test method comprises the following steps: the rabbit was acclimatized for 3 d. 24h before formal test, the two sides of the spine of the rabbit are sheared, the skin is not damaged, and the hair removing range is about 3cm multiplied by 3cm respectively at the left side and the right side. In the test, 0.5ml of the sample was applied directly to the left skin with an area of about 2.5cm × 2.5cm, and the right skin was used as a control and applied 1 time per day for 14 days. Hair was removed before each application from the next day, the remaining test substance was removed with warm water, the skin reaction was observed after removing the test substance for 1 hour, and the skin irritation/corrosion test was scored according to the skin irritation/corrosion test "table 1 skin irritation reaction score" of technical Specification for cosmetic safety (2015), and the control area and the test area were treated in the same manner. The average integral of each animal per day was calculated and the skin irritation intensity was judged by "skin irritation intensity grading of Table 2".

Results of multiple skin irritation tests of tested substances on rabbits

The average score of each animal was 0 every day, and no other symptoms of intoxication were observed. The result of the test on the skin irritation of the tested object to the rabbit for a plurality of times is nonirritant.

Thirdly, the method comprises the following steps: and (3) safety testing:

1. the inspection basis is as follows: sanitary chemistry inspection item in technical Specification for cosmetic safety (2015 edition).

(1) And (3) sanitary chemical inspection: mercury, lead, arsenic and cadmium all meet the requirements;

(2) and (3) microbial detection: the total number of colonies, the total number of moulds and yeasts, the heat-resistant coliform group, the staphylococcus aureus and the pseudomonas aeruginosa all meet the requirements, and the test results are as follows:

and (4) testing results:

results of microbiological examination

The product is subjected to toxicological tests, sanitary chemical tests and microbial tests at Shenzhen drug testing research institute, and all results meet the national standard. In particular toxicology tests, the results are the highest standards required by the state.

The foregoing examples are illustrative only, and serve to explain some of the features of the present disclosure. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. And that advances in science and technology will result in possible equivalents or sub-substitutes not currently contemplated for reasons of inaccuracy in language representation, and such changes should also be construed where possible to be covered by the appended claims.

Claims

1. The plant-extracted non-irritant itching-relieving repair liquid for the skin is characterized by comprising the following raw materials in percentage by weight: 0.04-20% of plant extract, 0.02-0.4% of polypeptide substance, 0.02-20% of lysozyme and the balance of water.

2. The plant-extracted non-irritating itching-relieving repair liquid according to claim 1, which is prepared from the following raw materials in percentage by weight: 5-15% of plant extract, 0.1-0.2% of polypeptide substance, 8-15% of lysozyme and the balance of water.

3. The plant-extracted non-irritating antipruritic liquid according to claim 2, wherein the plant extract is selected from one or more of the group consisting of a chamomile extract, a centella asiatica extract, and a mint extract.

4. The plant-extracted non-irritating antipruritic liquid according to claim 3, wherein the weight ratio of the chamomile extract to the centella asiatica extract to the mint extract is 1: (0.5-2.5): (0.05-0.5).

5. The plant-extracted non-irritating antipruritic liquid according to claim 4, wherein said camomile extract is extracted by a method comprising the steps of: (1) crushing golden chamomile to 200-mesh and 300-mesh, adding 60-85 wt% ethanol solution, and soaking for 15-24 h; (2) adding hydrochloric acid with the concentration of 5-10 mol% into the step (1) to adjust the pH value to 4-6, carrying out ultrasonic treatment for 1-2h, and filtering to obtain the compound.

6. The plant-extracted non-irritating itching-relieving repair liquid according to claim 5, wherein the mass ratio of the golden chamomile to the ethanol solution is 1: (5-15).

7. The plant-extracted non-irritating itching-relieving repair liquid as claimed in claim 5, wherein the ultrasonic extraction power is 800-1200W.

8. The plant-extracted non-irritating antipruritic liquid according to claim 5, wherein said polypeptide is tripeptide-1 copper.

9. A method for preparing a plant-derived non-irritating antipruritic liquid for skin use according to any one of claims 1 to 8, comprising the steps of: sequentially adding the plant extract, the polypeptide substances and the lysozyme into water, stirring and mixing uniformly, and filtering with 100-mesh and 300-mesh filter cloth.

10. Use of a plant-derived non-irritating antipruritic liquid according to any one of claims 1-8 for application to the skin of a young child.