KR20200040427A - Method for producing medicine of skin diseases - Google Patents

Abstract

The present invention relates to a method of preparing a naturopathic medication for skin and scalp diseases (psoriasis, atopic dermatitis, seborrheic dermatitis, eczema, vitiligo, hives, acute vesiculobullous hand eczema, acne, hair loss, damaged hair, scalp inflammation, and dandruff). In particular, as a first solution containing composite extracts extracted from fifteen types of plants, and a second solution containing Cordyceps militaris extracts are separately prepared, the naturopathic medication can be used to treat refractory skin and scalp diseases for the main ingredients of the first solution and the second solution. In the preparation process of the Cordyceps militaris extracts, the extracts are prepared to be completely free of hyphae, polymeric materials and the like through two concentrate liquid-forming processes and filtering processes. The pure Cordyceps militaris extracts thus produced further enhance the efficacy of the second solution and are further effective in treating skin diseases (psoriasis, atopic dermatitis, seborrheic dermatitis, eczema, vitiligo, hives, acute vesiculobullous hand eczema, acne, hair loss, damaged hair, scalp inflammation, and dandruff).

Description

Method and producing medicine of skin diseases for psoriasis, atopy, seborrheic dermatitis, eczema, vitiligo, hives, Korean herpes, acne, hair loss, hair damage, scalp inflammation, dandruff skin disease

The present invention relates to a method for preparing natural herbal medicines for skin and scalp diseases (psoriasis, atopy, seborrheic dermatitis, eczema, vitiligo, urticaria, acupuncture, acne, hair loss, hair damage, scalp inflammation, dandruff). More specifically, by separately preparing the first pharmaceutical solution containing the complex extract extracted from 15 plants and the second pharmaceutical solution containing the military cordyceps sinensis extract, the main components of the first pharmaceutical solution and the second pharmaceutical solution It can be used for the treatment of refractory skin and scalp diseases by, and by producing the extract in a state in which the mycelium and polymer substances are completely removed through two processes of concentration and filtration during the manufacturing process of the Cordyceps sinensis extract Skin and scalp diseases (psoriasis, atopy, seborrheic dermatitis), which can prepare the military cordyceps sinensis extract of the state, and thus can further improve the efficacy of the second pharmaceutical solution, and can be used more effectively in the treatment of skin diseases Natural eczema, vitiligo, hives, Hanpojin, acne, hair loss, hair damage, scalp inflammation, dandruff) About drug relates to a method of manufacturing the same.

In addition to atopy, the cause of most refractory skin diseases is based on genetic predisposition. However, in recent years, refractory skin diseases often occur or become more severe due to irregular diet and severe stress along with environmental pollution.

In other words, eating foods that are harmful to the body and being exposed to unfavorable living conditions in the human body, suffer from overwork and mental stress, and thus suffer from intractable skin diseases that are difficult to treat. In addition, the cause of such refractory skin disease is an important cause of weakening of the skin immune system due to drug abuse, such as antibiotics, in addition to environmental and food factors.

In particular, atopic dermatitis, asthma and hyperthermia as a result of allergic reactions to food and inhalable substances have been defined as "atopy" by Coca AF and Coke RA. , Asthma, allergic rhinitis, and allergic conjunctivitis. In particular, atopic dermatitis (AD) is a chronic recurrent skin disease that is often accompanied by allergic rhinitis or asthma, and is a very common skin disease that reports that 20% of newborn babies born in the year suffer from it. The disease is characterized by pruritus, dryness of the skin, hyperactivity around the hair follicles, stomatitis, lichenification, and eczema lesions.

There are several hypotheses about the causes of atopic dermatitis, but the exact cause is unknown, and it is believed that genetic and immunological factors, adverse drug reactions, environmental factors, and microorganisms will be involved. Recently, abnormal increases in serum IgE levels in patients with atopic dermatitis, number of T lymphocytes that play a central role in cell immunity, and functional disorders have been reported.

The goal of atopic dermatitis treatment is to reduce the exacerbation factor while reducing pruritus and dermatitis, along with adequate hydration for dry skin. Currently, the treatments used in both rooms are very diverse, such as identifying and avoiding the causes of exacerbation, using antihistamines or steroids, treating anti-inflammatory drugs, and administering immunomodulators, but these treatments have problems such as multiple side effects when used for a long time.

Therefore, in recent years, many studies on natural products capable of effectively and safely treating side effects with minimal side effects have been actively conducted.

Domestic registered patent No. 10-1029534

The present invention was invented to solve the problems of the prior art, and the object of the present invention is to separate the first pharmaceutical solution containing the complex extract extracted from 15 plants and the second pharmaceutical solution containing the military cordyceps sinensis extract. Skin and scalp diseases (psoriasis, atopy, seborrheic dermatitis), which can be prepared and used in the treatment of refractory skin diseases by the main components of the first pharmaceutical solution and the second pharmaceutical solution by mixing dilution with purified water together with other solutions It provides a method of manufacturing natural herbal medicines for eczema, vitiligo, hives, hanpojin, acne, hair loss, hair damage, scalp inflammation, dandruff).

Another object of the present invention is to produce the extract in a state in which the mycelium and the polymer material are completely removed through two processes of concentration and filtration during the manufacturing process of the Cordyceps sinensis extract, Skin and scalp diseases (psoriasis, atopy, seborrheic dermatitis, eczema, vitiligo, urticaria, herpes, which can be prepared and thus can further improve the efficacy of the second pharmaceutical solution, and can be used more effectively for the treatment of skin diseases , Acne, hair loss, hair damage, scalp inflammation, dandruff) to provide a method of manufacturing natural herbal treatments.

The present invention comprises the steps of preparing a first pharmaceutical solution by mixing a complex extract extracted from a number of plants in purified water and a butylene glycol mixed solvent; Preparing a second pharmaceutical solution by mixing the military cordyceps sinensis extract with methylpropanediol in a ratio of 0.03: 9.97 by weight; Mixing the gypsum powder with purified water in a ratio of 0.05: 10 weight to generate a gypsum leaching solution; Mixing the alum powder with purified water in a ratio of 0.001: 10 weight to produce a solution of alum leach; And mixing the first drug solution, the second drug solution, colloidal silver, the gypsum leaching solution, and the vitiligo leaching solution in purified water at a weight ratio of 37.949 in a weight ratio of 30: 10: 2: 10.05: 100.001. The first pharmaceutical solution is prepared by mixing the complex extract in a 1.5 weight ratio in a mixed solvent in which purified water and butylene glycol are mixed in a 19.5: 9 weight ratio. Extract, Ginseng Extract, Gold Silver Coin Extract, Celandine Grass Extract, Ringworm Extract, Loquat Leaf Extract, Eoseongcho Extract, Purple Root Extract, Violet Flower Extract, Oil Extract, Gentian Extract, Cheongho Extract, Poongyoung Extract, Motherwort Extract 0.1 : 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.03 Skin and scalp characterized by being mixed by weight ratio Provides a method of manufacturing natural herbal remedies for psoriasis (psoriasis, atopy, seborrheic dermatitis, eczema, vitiligo, hives, Korean herpes, acne, hair loss, hair damage, scalp inflammation, dandruff).

At this time, a plurality of plant extracts mixed in the complex extract, each step of crushing the hay of the plant, and the mixture produced by mixing the crushed hay with an extraction solvent for 50 to 60 ℃ range for 2 to 5 hours Through the step of stirring, the step of cooling after the stirring step to remove and remove the residue, and the step of producing a concentrate using a vacuum concentrator after the step of filtering, and the step of treating the resulting concentrate with activated clay Each can be manufactured.

In addition, the method for preparing the Cordyceps sinensis extract of Militaryis, culturing the strains of Cordyceps sinensis and fermenting the cultured strains at 27 ° C for 4 days to produce a fermentation broth, and mycelium from the fermentation broth using a centrifugal separator And removing the mycelia, concentrating the fermentation broth from which the mycelium has been removed using a vacuum concentrator to produce a concentrate, and filtering the concentrate through an ultrafiltration membrane to remove the polymer material, and decompressing the concentrate from which the polymer material has been removed. Reconcentration using a concentrator may include regenerating the concentrate.

According to the present invention, the first pharmaceutical solution containing the complex extract extracted from 15 plants and the second pharmaceutical solution containing the military cordyceps sinensis extract are separately prepared and mixed and diluted in purified water together with other solutions to remove the first pharmaceutical solution. Intractable skin and scalp diseases, such as psoriasis, atopy, seborrheic dermatitis, eczema, vitiligo, urticaria, herpes zoster, acne, hair loss, hair damage, scalp inflammation, by the main components of the 1st drug solution and the 2nd drug solution, It can be used for the treatment of dandruff, and in addition to the treatment of hair loss, it can also be made to exhibit a wool function.

In addition, by producing the extract in a state in which the mycelium and the polymer material are completely removed through two concentrations and a filtration process in the manufacturing process of the Cordyceps sinensis extract of Militaris, the extract of Cordyceps sinensis in the pure state can be produced. , Accordingly, it is possible to further improve the efficacy of the second pharmaceutical solution, and has an effect that can be more effectively used for treating skin and scalp diseases.

1 is a manufacturing flow chart showing a method of manufacturing a therapeutic agent for skin and scalp diseases according to an embodiment of the present invention step by step,
Figure 2 is a manufacturing flow diagram showing a method of manufacturing a first pharmaceutical solution according to an embodiment of the present invention step by step,
3 is a manufacturing flow chart showing a method of manufacturing a second pharmaceutical solution according to an embodiment of the present invention step by step.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. First, when adding reference numerals to the components of each drawing, it should be noted that the same components have the same reference numerals as possible even though they are displayed on different drawings. In addition, in describing the present invention, when it is determined that detailed descriptions of related well-known structures or functions may obscure the subject matter of the present invention, detailed descriptions thereof will be omitted.

1 is a manufacturing flow chart showing a method of manufacturing a therapeutic agent for skin and scalp diseases according to an embodiment of the present invention step by step, and FIG. 2 is a method showing a first pharmaceutical solution manufacturing method according to an embodiment of the present invention step by step It is a manufacturing flow chart, and FIG. 3 is a manufacturing flow chart showing a method for manufacturing a second pharmaceutical solution according to an embodiment of the present invention step by step.

A method of manufacturing a therapeutic agent for skin and scalp disease according to an embodiment of the present invention comprises the steps of preparing a first pharmaceutical solution by mixing a complex extract extracted from a number of plants with purified water and a mixture of butylene glycol (S10) and milli Mixing the Tarris Cordyceps sinensis extract with methylpropanediol solvent to prepare a second pharmaceutical solution (S20), mixing gypsum powder with purified water to generate a gypsum leaching solution (S30), and mixing alum powder with purified water Including the step (S40) of producing a vitiligo leaching solution, a first pharmaceutical solution, a second pharmaceutical solution, colloidal silver, a plaster leaching solution, and mixing the alum leaching solution with purified water (S50) It is composed.

The therapeutic agent for skin and scalp diseases manufactured as described above may be used as a therapeutic agent for inflammatory diseases, atopic diseases, or psoriasis. More specifically, it can be used as a treatment for refractory skin diseases such as psoriasis, atopy, eczema, hanpojin, acne, hives, seborrheic dermatitis, vitiligo, and also as a treatment for hair loss, hair damage, scalp inflammation, and dandruff. , In addition to the treatment of hair loss, it can also be used as a therapeutic agent that can also exhibit wool function.

Step (S10) for preparing the first pharmaceutical solution is prepared by mixing a complex extract (extract mixture extracted from multiple plants) in a 1.5 weight ratio in a mixed solvent in which purified water and butylene glycol are mixed in a 19.5: 9 weight ratio. In this case, the complex extracts include, gusset extract, ginseng extract, gold silver coin extract, celandine grass extract, ringworm extract, loquat leaf extract, Eoseongcho extract, purple root extract, violet extract, oil-fat extract, gentian extract, cheongho extract, pogongyoung extract, It is prepared by mixing motherwort extract and Hwangnyeon extract in a weight ratio of 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.03.

The Portulaca Oleracea Extract is extracted from the Portulaca oleracea, that is, the outpost of purslane, which is one year from the purslane. Machi Prefecture extract contains l-norepinephrine, vitamins A, B1, B2, C and tocopherols, carotene, omega-3 fatty acids, glutathione, glutamic acid, malic acid, citric acid, etc. Due to its action, it has been used for various skin diseases since ancient times. In Donguibogam, Machihyeon is cold and tasteless and has no poison to cure various boils and stools, facilitate bowel movements, treat boils and injuries caused by iron injuries, cure thirst, and quench thirst It is said to kill eggplant worms. In addition, Machihyeon is used for various diseases such as detoxification, dysentery, swelling, and uterine contraction of insect poison, paralysis, diuretic drugs, and poison. It has been reported in the Middle East as a diuretic, anthelmintic and skin sedative. Other studies on muscle relaxation activity and anticancer effects have also been reported. In Korea, related patent technologies for anti-obesity components, anti-obesity components and athlete's foot treatment compositions have been disclosed. In the present invention, Machihyeon extract is effective for skin moisturizing, anti-inflammatory effect, skin cleansing, anti-allergic, antibacterial effect, atopic and dry skin, and has effects such as skin aging relief, skin irritation relief, and active ingredients such as omega-3 It has a related effect that works depending on the content.

Gosam refers to Sophora flavescens, a perennial perennial plant, and can also be used to refer to the root part of a thief's staff. The ginseng is used for dysentery, hypodermia, genital pruritus, and itching of the skin due to hypothermic heat, and is used when there is pain in the urine due to bladder heat. In the present invention, the ginseng extract has an effect related to preventing skin itching.

Gold and silver coins are buds of the honeysuckle (Lonicera japonica Thunberg) or its varieties. The name In-dong was created because the vines are alive and do not wither even in cold winters. The name Gold-eun-hwa is the name of the first blooming flower, but it gradually turns yellow. This medicine has a peculiar smell, sweet taste and cold properties. Gold and silver coins are used when the fever is lowered and the chest is stuffy and thirsty. It is good for inflammation, and causes boils, skin-poisoned poisons, organ inflammation, and discharge. It is also used for dysentery, necrosis of skin tissue due to heat poisoning, mastitis, etc. It is applied to colitis, gastric ulcer, cystitis, sore throat, tonsillitis, bronchitis, conjunctivitis and swelling, high fever due to mumps, purulent infections, etc. The pharmacological action has been reported to be antibacterial, anti-inflammatory, antipyretic, leukocyte phagocytosis, central nervous system excitation, serum cholesterol lowering, and ulcer prevention. In the present invention, the gold and silver extract has a detoxifying effect on the skin.

The celandine (Chelidonium Majus L.) is a plant of the Papaveraceae family. It is also called seed shit and milkweed, and is called Baekgulchae in Chinese medicine. The scientific name Chelidonium is derived from the Greek word chelidon (swallow) because when the swallow chicks are born, there are many foreign substances in the eyes, so the mother cannot open her eyes. . Ingredients include alkaloids, berberine, chelerythrine, chelidonine, citra acid, formic acid, malic acid, nicotinic acid, protopin, sanguinarine, etc. Has been used for skin diseases, various cancers, eye diseases, arthritis, athlete's foot, gastritis, stomach cramps, stomach ulcers, hepatitis, abdominal pain, and digestive diseases in oriental medicine and civilians

Ringworm skin refers to the root bark of Ringworm (Dictamnus dasycarpus Turcz.). Ringworm skin is used for skin rash due to moist heat, skin erosion, eczema, rubella, itching, allergic dermatitis, jaundice due to neurodermatitis and acute hepatitis, and can be used for seawater, throat drying, alternation, and pharmacological effects. Antipyretic and skin fungal inhibitory effects are known.

It is known that Eriobotrya Japonica Leaf contains ingredients such as amiddaline, ursolic acid, olenolic acid, tannin, and vitamin B1. In addition, the loquat leaf is described as a prescription drug to treat the occurrence of convulsions and vain areas in “medical odor” <main rear>, and in <Korean Anti-Cancer Herb> is described as a tree that improves various cancers.

Eoseongcho refers to the upper part of the flowering period of Houttuynia cordata Thunberg. Eoseongcho is a name given to the fishy smell of the leaves. This medicine has a fishy smell when it is crushed by rubbing the leaves, and the taste is spicy and slightly cold. Eoseongcho has excellent antipyretic and draining action, and is used for coughing and vomiting due to lung abscess, pneumonia, acute bronchitis, enteritis, urinary tract infection, and boil. It is used when there is a lot of heat and cannot urinate. As a pharmacological action, antibacterial action, immune enhancing action, anti-inflammatory action, diuretic action, antitussive action have been reported.

Lithospermi Radix is the root of the Boraginaceae family, such as Lithospermum erythrorhizon Siebold et Zuccarini, Arnebia euchroma Johnst, or Arnebia guttata Bunge. It is 6 to 10 cm long and 5 to 15 mm in diameter. The outer surface is dark purple to purple brown, and the skin is rough and easy to peel off. Most of them have twisted deep vertical grooves, sometimes reaching the neck. Sometimes the roots of the stem are attached to the root. It is easy to bend and the bent side is granular and has many gaps. When looking at the cross section through a magnifying glass, the outer part of the skin is dark purple, the inner light brown part is irregularly arranged, and the neck is close to yellow. The center of the root head is sometimes hollow, and the area around it is reddish purple. This medicine has a slight characteristic odor and a slightly sweet taste. It is known to be used as a purple dye, to lower heat, clear blood, and improve blood circulation.

Violet is a perennial plant that commonly grows on a sunny meadow or hillside in the spring, and its ingredients include essential oils (methyl salicylate), disaccharides, flavonoids, saponins, and pigments. Since the pharmacological effect is excellent for purulent diseases, it has antiseptic, antipyretic, and anti-inflammatory effects on boils, lymph nodes, tuberculosis, etc. It has detoxification, anti-inflammatory, edema, branch, end-diuresis, and diuretic properties, so it is used for jaundice, hepatitis, and edema and is used as a fragrance.

Jigui (Sanguisorba officinalis) is a grass that smells like cucumber or watermelon when you open the leaf and put it on your nose. It is also called cucumber grass because it has a thicker cucumber smell than real cucumber, and its roots are thick and hard. At the end of the long petiole, there are 5 ~ 13 small leaves, the shape of the leaves is long oval, the ends are blunt, and the edges are coarse, and the oily leaves contain trace elements such as carbohydrate, protein, fat and calcium, iron, copper, and zinc. Evenly, it is good to eat with herbs. Flowers bloom in July-October, seeds ripen in August-November, and can be used as medicine by cutting roots in the fall and cutting them into small pieces. This oil is a folk medicine that is important for diarrhea, colitis, bleeding, spear wounds, and burns. It is especially used to bleed various bleeding due to its strong hemostatic action. Just as applying cucumber juice to burns is effective, it can also be used for burns by crushing the leaves, roots, and stems of the oil that produces cucumber. Jiyu also has the effect of stopping the blood, so it is mixed with blood in the stool. It is also used for uterine bleeding, excessive menstruation, and intestinal bleeding.

Yongdam (Gentiana scabra) is a perennial plant of Yongdammok Yongdam, a dicotyledonous group. Since ancient times, it has been used for symptoms such as antibacterial and anti-inflammatory effects, and indigestion.

Cheongho (Art, Artemisiae apiaceae Herba) is a herbaceous plant of Artemisia apiacea HANCE and A. annua L., which are the first or second-year plants belonging to the Asteraceae family. It grows naturally in rivers, riversides, and coastal areas. It has been known to have antibacterial, antipyretic, hemostatic, and analgesic effects mainly on skin fungi. Also, food supplements and Japanese anecdotes are used to help the growth of hair or to help hair grow. It has been described as having the effect of blackening.

Pogongyoung is a dried herb of Dandelion (Taraxacum platycarpum H. Dahlstedt) of the Asteraceae family, or the same plant, and is also known as Geumjamcho because the flower looks like the head of a gold secretary. In oriental medicine, pogongyoung has been used for the treatment of fever, sweating, dry stomach, tonic, detoxification, lymphadenitis, acute bronchitis, gastritis, hepatitis, cholecystitis, gynecological diseases. Since ancient times, dandelion has been used as a herb and food in Korea as well as in Europe, and root is used as a substitute for coffee. In China and North America, it has been known as a traditional folk medicine and has been used for wound healing. In recent years, not only is it edible as raw vegetables, but its consumption is gradually increasing as a health food ingredient. Representative active ingredients of Pogong-young have been reported as taraxastenol, taraxarol, and taraxerol. According to recent research results, there are ingredients that inhibit carcinogens, and it is found that it is rich in organic germanium and is also effective in preventing cancer.

Motherwort is a medicinal herb that dried the upper part of the motherwort (Leonurus japonicus). Ingredients of motherwort include alkaloids (leonurine, leonuridine, and starhydrin) in the outpost, and include phytosterin, essential oil, saponin, oil, resin, rutin, organic acid, and coumarin. Due to its pharmacological action, the water extract has a calming effect on the central nervous system. Leonurin lowers blood pressure, heart, and diuretics. Leonurin and starhydrin strain the uterus of experimental animals and increase the number of contractions, while starhydrin exhibits more action on the uterus than leonurin, whereas leonurin is more sedative, lower in blood pressure, stronger, diuretic than starhydrin It shows action.

As a vernacular herbaceous perennial plant of the family Ranunculus (Coptis japonica Makino), it has been found that the extract of Hwangnyeon, which is used as an active ingredient, has a nitrite scavenging effect, which is a cancer-causing substance. It is known to suppress the activity of various harmful bacteria as an effect. In addition, it is known to help maintain healthy skin by preventing secondary infection and damage of the skin, as well as protecting the skin from harmful oxygen attacks that cause skin aging and serious diseases. These hwangnyeon extracts are mixed at a relatively higher weight ratio than the other 14 plant extracts.

As such, the complex extract is composed of 15 plant extracts, each of which is useful for skin health or has an effect of detoxification, and can be used as a natural extract without side effects in the treatment of various skin diseases.

The extract for each of these plants is a step (S11) of crushing the hay of the respective plants as shown in FIG. 2, and the mixture produced by mixing the crushed hay with an extraction solvent (ethanol, etc.) at 50-60 ° C. Stirring for 2 to 5 hours in the range (S12), after stirring, cooling it and removing the residue with 400 mesh and filtering (S13), and after filtering, using a reduced pressure concentrator to produce a concentrate It is prepared through the steps (S14) and the step (S15) of treating the resulting concentrate with activated clay. Thereafter, it may be prepared in a powder form through a separate freeze dryer, but in one embodiment of the present invention, a natural extract can be obtained in the form of a concentrate.

The step (S20) of preparing the second pharmaceutical solution proceeds by mixing the military Cordyceps sinensis extract with a methylpropanediol solvent in a ratio of 0.03: 9.97 by weight.

The Cordyceps militaris is a small mushroom that puts fruit on the body of an insect, some of which are known depending on the host insect. It is mainly used for symptoms of reluctance of cold when sweating after colds after chronic illness, waist, knee pain, lower body weakness, oil well, dream, premature ejaculation, tinnitus, forgetfulness, ecstasy, rash, seawater, and chronic illness. The main component of the military cordyceps is known as cordycepin, and has been reported to have immune-enhancing and anti-allergic effects.

The method of manufacturing the militaryis Cordyceps sinensis extract, as shown in FIG. 3, cultivating the militaryis Cordyceps sinensis (S21), and fermenting the cultured strains at 27 ° C for 4 days to produce a fermentation broth (S22). , Removing the mycelium from the fermentation broth using a centrifugal separator (S23), and concentrating the fermentation broth from which the mycelium has been removed using a concentrator under reduced pressure (S24), and filtering the concentrate through an ultrafiltration membrane to polymer material It comprises a step (S25) of removing, and re-concentrating the concentrate from which the polymer material is removed using a reduced pressure concentrator to regenerate the concentrate (S26).

A centrifuge is used to remove the mycelium, a concentrate is generated after removal of the mycelium, and then the polymer material is removed again using an ultrafiltration membrane, and the concentrate is regenerated through a reconcentration process, thereby completely preventing foreign substances such as impurities. It is possible to prepare pure military extract of Cordyceps sinensis.

Since the active ingredient of Militaris cordyceps, which is prepared in this way, does not readily dissolve in water, and when dissolved in water, there is a problem such as high polarity or the need to add a preservative such as phenoxyethanol, so one embodiment of the present invention In, a second pharmaceutical solution is prepared by mixing and diluting the Military Lees Cordyceps sinensis extract with methylpropanediol solvent.

Colloidal Silver refers to a state in which silver particles of nanometer size, that is, 0.0015 to 0.15 μm, are suspended in a liquid such as water or alcohol. These colloidal silver are natural antibiotics, various skin disease treatment agents, It is used as a disinfectant, a burn remedy, and the like.

Gypsum is known to relieve the symptoms of cold, spicy, and non-toxic, sore throat due to rot, fever in the body, fever in 3 seconds, skin fever, dry mouth, tongue burning, and burning of the throat. Alum is cold in nature, tastes sour, and has no poison. It cuts the walls, swells the dysentery, heals the food and spears, removes the nasal congestion, and heals a sudden blockage in the throat. It strengthens the bones and teeth, and heals the rest, palsy, and scabies. Heals various boils, but removes the bad and makes the new flesh alive.

Since the oriental medicine efficacy of such gypsum and alum is expected, in one embodiment of the present invention, the gypsum powder is mixed with purified water in a ratio of 0.05: 10 to produce a gypsum leaching solution, and the alum powder is mixed with purified water in a ratio of 0.001: 10 by weight. To produce a vitiligo leaching solution, a very small amount of the gypsum leaching solution and the vitiligo leaching solution is added.

Skin disease treatment method according to an embodiment of the present invention, the first pharmaceutical solution, the second pharmaceutical solution, colloidal silver, plaster leaching solution and vitiligo leaching solution described above are sequentially mixed with purified water in skin and scalp diseases It has a therapeutic effect, and after the mixing process, the impurities are removed through a separate filtration process, and finally the preparation of a therapeutic agent for skin and scalp diseases is completed. The manufactured skin and scalp disease treatment agent is mixed with actual products such as cosmetics, so that cosmetics and the like can have skin and scalp disease treatment efficacy.

-Example-

Purified water and butylene glycol in a mixed solvent mixed in a weight ratio of 19.5: 9, gusset extract, ginseng extract, gold and silver extract, celandine extract, ringworm extract, loquat leaf extract, eoseongcho extract, purple root extract, violet extract, oil-based extract , Yongdam extract, Cheongho extract, Poongyoung extract, motherwort extract, Hwangnyeon extract 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.03 Weight ratio complex The first pharmaceutical solution is prepared by mixing the extract in a 1.5 weight ratio. Here, each plant extract mixed with the complex extract is mixed with crushed hay in ethanol in a weight ratio of 0.2: 1 to stir the resulting mixture for 3 hours in the range of 55 ° C, and after stirring it is cooled to 6 ° C and the residue Remove and filter with 400 mesh. After the step of filtration, a concentrated solution is produced using a reduced pressure concentrator, and the resulting concentrated solution is prepared by treatment with activated clay. Thereafter, it may be prepared in a powder form through a separate freeze dryer, but in one embodiment of the present invention, a natural extract can be obtained in the form of a concentrate.

The second pharmaceutical solution is prepared by mixing the extract of military cordyceps sinensis with methylpropanediol in a ratio of 0.03: 9.97 weight, and the military extract of Cordyceps sinensis is cultured by inoculating the military cordyceps sinensis, and the cultured strain is 4 at 27 ° C. After fermentation for a day to produce a fermentation broth, the mycelium is removed from the fermentation broth using a centrifuge. The fermentation broth from which the mycelium has been removed is concentrated using a vacuum concentrator to produce a concentrate, and the concentrate is filtered through an ultrafiltration membrane to remove the polymer material, and the concentrate from which the polymer material is removed is reconcentrated using a vacuum concentrator to regenerate the concentrate.

Gypsum powder is mixed with purified water in a ratio of 0.05 to 10 weight to produce a gypsum leaching solution, and alum powder is mixed with purified water in a ratio of 0.001 to 10 weight to produce a white leaching solution.

The first pharmaceutical solution, the second pharmaceutical solution, colloidal silver, gypsum leaching solution, and alum leaching solution 30: 10: 2: 10.05: 10.001 in a weight ratio of 37.949 weight ratio of purified water according to the present invention Prepare a skin disease treatment.

-Treatment efficacy Experimental example -

Toxicity analysis for HaCaT cells

Before analyzing the anti-inflammatory activity of a skin disease treatment agent (hereinafter referred to as HM-V sample) according to an embodiment of the present invention targeting human keratinocytes HaCaT cells, the cytotoxicity of the HM-V sample itself was confirmed. For the analysis of cytotoxicity, a commonly used MTT assay method was used, and HM-V samples were diluted in serum-free medium for each concentration (02-10%), treated on cells for 24 hours, and analyzed, FIG. 4 As shown in the HM-V was confirmed to be toxic to HaCaT cells from a concentration of 3% or more.

Skin inflammatory factor / inflammatory cytokine gene expression inhibition analysis

In the anti-inflammatory activity analysis, we focused on atopic dermatitis to analyze the activity of HM-V, and when treated with TNF-α and IFN-γ (each 10 ng / mL), which are inflammation-inducing substances in vitro, HaCaT cells related to skin inflammation It is known that the expressions of the factors CCL17, 22 and inflammatory cytokines increase, and it was intended to confirm the activity of inhibiting the expression of these genes by HM-V.

The reverse transcription-polymerase chain reaction (RT-PCR) method was used for gene expression analysis, and HM-V samples were diluted in serum-free medium for each concentration (075-3%), together with TNF-α and IFN-γ. As a result of analyzing the mRNA after treatment with cells for a period of time, it was confirmed that HM-V inhibits the expression of CCL17, CCL22 and inflammatory cytokine genes in proportion to the treatment concentration, as shown in FIG. 5.

Analysis of AD improvement efficacy by HM-V

The mouse used in the animal experiment is a 'SKH-1 hiarless' species, and has almost no hair, which is convenient for intuitively observing the condition of the skin. In total, 20 groups of 3 groups (6 vehicles / 7 DNCBs / DNCB +) HM-V 7 individuals). After an adaptation period of about 1 to 2 weeks, 100 μL of DNCB (2,4-dinitrochlorobenzen) prepared at a concentration of 1% in acetone: olive oil (3: 1) solution was applied to the skin of mice and the like at regular times every day. After 1 week, when the induction of AD (atopic dermatitis) is confirmed, DNCB is lowered to 0.5% and applied in 100 μL intervals every 2 days, and HM-V is prepared at a concentration of 3% in physiological saline and 100 μL daily Treatment for 6 weeks. During the experiment, the condition of the skin was observed weekly (see FIG. 6), and the thickness change was observed by measuring the skin thickness at the same site (see FIG. 7).

After the adaptation period of the experimental animals, 1% of DNCB was treated every day for a week for a week except for the vehicle group, and as a result, it was confirmed that AD was severely induced in all the treated individuals. After confirming AD induction, in order to maintain AD symptoms, DNCB was lowered to 05% and treated with HM-V daily at a concentration of 3% while being treated at 2-day intervals. As a result, it was confirmed that HM-V significantly alleviated AD symptoms from a short treatment period of about a week, and improved AD symptoms to almost the same level as normal skin in treatment for a total of 6 weeks.

When atopic dermatitis progresses, keratinization of the skin occurs and the thickness of the skin is accompanied. As a result of measuring the skin thickness according to the duration of HM-V treatment in the AD model inducing contact dermatitis with DNCB, it was confirmed that the skin thickness increased by AD gradually decreased with HM-V treatment. It can be seen that the keratinization of the skin by AD is significantly improved by HM-V.

Analysis of inflammatory inhibitory activity of HM-V

At the expense of the animal model of contact dermatitis, the spleen and lymph nodes, which are the main organs involved in the immune response, were separated and analyzed by comparing the size of each organ.

As shown in FIG. 8, when comparing the size of each organ with the vehicle group, it was confirmed that immune cells were deposited in the spleen and lymph nodes in the DNCB-inducing group, resulting in a significant increase in size, whereas in the HM-V-treated group, DNCB induction It was confirmed that the size of the spleen and lymph nodes was significantly reduced as compared to the vehicle group compared to the group.

These results confirm that HM-V can suppress the overall inflammatory response well, in addition to silk atopic dermatitis, and show the possibility of being used as a hyperimmune inhibitor.

The above description is merely illustrative of the technical idea of the present invention, and those skilled in the art to which the present invention pertains may make various modifications and variations without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical spirit of the present invention, but to explain, and the scope of the technical spirit of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the claims below, and all technical spirits within the scope equivalent thereto should be interpreted as being included in the scope of the present invention.

Claims (1)

Preparing a first pharmaceutical solution by mixing a complex extract extracted from a number of plants with purified water and a butylene glycol mixed solvent;
Preparing a second pharmaceutical solution by mixing the military cordyceps sinensis extract with methylpropanediol in a ratio of 0.03: 9.97 by weight;
Mixing the gypsum powder with purified water in a ratio of 0.05: 10 weight to generate a gypsum leaching solution;
Mixing the alum powder with purified water in a ratio of 0.001: 10 weight to produce a solution of alum leach; And
Mixing the first pharmaceutical solution, the second pharmaceutical solution, colloidal silver, the gypsum leaching solution, and the vitiligo leaching solution in purified water at 37.949 weight ratio in a weight ratio of 30: 10: 2: 10.05: 100.01 step
Including, the first pharmaceutical solution is prepared by mixing the complex extract in a 1.5 weight ratio in a mixed solvent in which purified water and butylene glycol are mixed in a 19.5: 9 weight ratio, and the complex extract is a gusset extract, High ginseng extract, gold silver coin extract, celandine grass extract, ringworm extract, loquat leaf extract, fish vine extract, purple root extract, violet extract, oil extract, gentian extract, cheongho extract, poongyoung extract, motherwort extract, cornflower extract 0.105 0.105: 0.105 : 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.105: 0.03
A plurality of plant extracts mixed in the complex extract is
Each step of crushing the hay of the plant, and mixing the crushed hay with an extraction solvent to stir the resulting mixture in the range of 50-60 ° C. for 2 to 5 hours, and after the stirring step, cool it and remain. It is prepared through the step of removing and filtering water, the step of generating a concentrate using a vacuum concentrator after the step of filtering, and the step of treating the resulting concentrate with activated clay,
The method of manufacturing the military iris cordyceps extract
Cultivating the military iris cordyceps, fermenting the cultured seed for 4 days at 27 ° C. to produce a fermentation broth, removing the mycelium from the fermentation broth using a centrifugal separator, and decompressing the fermentation broth with the mycelium removed Concentrating to produce a concentrate, and filtering the concentrate through an ultrafiltration membrane to remove the polymer material, and reconcentrating the concentrate from which the polymer material is removed using a reduced pressure concentrator to regenerate the concentrate. Method of manufacturing a natural herbal medicine for skin diseases, characterized in that.

Images