CN110658617B - 使用校正因子的显微成像方法 - Google Patents

使用校正因子的显微成像方法 Download PDF

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Publication number
CN110658617B
CN110658617B CN201910558190.1A CN201910558190A CN110658617B CN 110658617 B CN110658617 B CN 110658617B CN 201910558190 A CN201910558190 A CN 201910558190A CN 110658617 B CN110658617 B CN 110658617B
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image
signal
sample
correction factor
corrected
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Chinese (zh)
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CN110658617A (zh
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V.德雷舍
N.兰霍尔兹
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Carl Zeiss Microscopy GmbH
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration using two or more images, e.g. averaging or subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/95—Computational photography systems, e.g. light-field imaging systems
    • H04N23/951—Computational photography systems, e.g. light-field imaging systems by using two or more images to influence resolution, frame rate or aspect ratio
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N5/00—Details of television systems
    • H04N5/30—Transforming light or analogous information into electric information
    • H04N5/32—Transforming X-rays
    • H04N5/3205—Transforming X-rays using subtraction imaging techniques
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10148—Varying focus
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20216—Image averaging
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction

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  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Computing Systems (AREA)
  • Microscoopes, Condenser (AREA)
CN201910558190.1A 2018-06-28 2019-06-25 使用校正因子的显微成像方法 Active CN110658617B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102018210606.1 2018-06-28
DE102018210606.1A DE102018210606B4 (de) 2018-06-28 2018-06-28 Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors

Publications (2)

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CN110658617A CN110658617A (zh) 2020-01-07
CN110658617B true CN110658617B (zh) 2023-10-20

Family

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CN201910558190.1A Active CN110658617B (zh) 2018-06-28 2019-06-25 使用校正因子的显微成像方法

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US (1) US11238575B2 (enExample)
JP (1) JP7254643B2 (enExample)
CN (1) CN110658617B (enExample)
DE (1) DE102018210606B4 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102023102991B3 (de) * 2023-02-08 2024-02-01 Till I.D. Gmbh Verfahren zur Generierung mikroskopischer Schichtaufnahmen 3-dimensionaler fluoreszierender Objekte sowie Vorrichtung, Computerprogramm und computerlesbares Speichermedium

Citations (5)

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JP2000275540A (ja) * 1999-03-24 2000-10-06 Olympus Optical Co Ltd 共焦点顕微鏡
CN1410744A (zh) * 1996-08-16 2003-04-16 图像研究公司 用于分析井板、凝胶和斑点的数字成像系统
CN103926228A (zh) * 2014-04-28 2014-07-16 江苏天宁光子科技有限公司 一种激光扫描共焦荧光显微内窥成像系统
WO2017148555A1 (de) * 2016-03-02 2017-09-08 Carl Zeiss Microscopy Gmbh Verfahren zur bestimmung einer höhenlage eines objekts
CN107228846A (zh) * 2017-05-10 2017-10-03 暨南大学 基于离轴光束焦面共轭的荧光成像光切片方法和装置

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US5923430A (en) * 1993-06-17 1999-07-13 Ultrapointe Corporation Method for characterizing defects on semiconductor wafers
US5923465A (en) * 1994-10-28 1999-07-13 Marc J. Byrd System for scanning confocal image reconstruction from coherent recordings
GB9603788D0 (en) 1996-02-22 1996-04-24 Isis Innovation Confocal microscope
DE19824460A1 (de) * 1998-05-30 1999-12-02 Zeiss Carl Jena Gmbh Anordnung und Verfahren zur mikroskopischen Erzeugung von Objektbildern
GB9901365D0 (en) * 1999-01-22 1999-03-10 Isis Innovations Ltd Confocal microscopy apparatus and method
US6426835B1 (en) * 1999-03-23 2002-07-30 Olympus Optical Co., Ltd. Confocal microscope
US6341035B1 (en) * 1999-07-09 2002-01-22 Olympus Optical Co., Ltd. Confocal microscope
GB2401431B (en) * 2003-04-02 2005-04-27 Amersham Biosciences Uk Ltd Method of, and computer software for, classification of cells into subpopulations
US7170675B2 (en) * 2004-05-19 2007-01-30 Celloptic, Inc. Method and system for wide-field multi-photon microscopy having a confocal excitation plane
US8542899B2 (en) * 2006-11-30 2013-09-24 Definiens Ag Automatic image analysis and quantification for fluorescence in situ hybridization
GB201007055D0 (en) * 2010-04-28 2010-06-09 Vib Vzw Method and apparatus for the imaging of a labelled sample
US10006922B2 (en) * 2011-12-22 2018-06-26 Massachusetts Institute Of Technology Raman spectroscopy for detection of glycated analytes
WO2013109957A1 (en) * 2012-01-18 2013-07-25 University Of Utah Research Foundation Devices and systems for fluorescence imaging of tissue
US9449377B2 (en) * 2012-10-09 2016-09-20 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Imaging methods and computer-readable media
DE102013218795A1 (de) * 2013-09-19 2015-03-19 Carl Zeiss Microscopy Gmbh Laserscanningmikroskop und Verfahren zur Korrektur von Abbildungsfehlern insbesondere in der hochauflösenden Scanning-Mikroskopie
DE102014004249A1 (de) 2014-03-24 2015-09-24 Carl Zeiss Microscopy Gmbh Konfokales Mikroskop mit Aperturkorrelation
US10129448B2 (en) * 2016-07-20 2018-11-13 Dental Imaging Technologies Corporation Optical coherence tomography imaging system

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410744A (zh) * 1996-08-16 2003-04-16 图像研究公司 用于分析井板、凝胶和斑点的数字成像系统
JP2000275540A (ja) * 1999-03-24 2000-10-06 Olympus Optical Co Ltd 共焦点顕微鏡
CN103926228A (zh) * 2014-04-28 2014-07-16 江苏天宁光子科技有限公司 一种激光扫描共焦荧光显微内窥成像系统
WO2017148555A1 (de) * 2016-03-02 2017-09-08 Carl Zeiss Microscopy Gmbh Verfahren zur bestimmung einer höhenlage eines objekts
CN107228846A (zh) * 2017-05-10 2017-10-03 暨南大学 基于离轴光束焦面共轭的荧光成像光切片方法和装置

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Publication number Publication date
JP7254643B2 (ja) 2023-04-10
JP2020003793A (ja) 2020-01-09
DE102018210606A1 (de) 2020-01-02
CN110658617A (zh) 2020-01-07
DE102018210606B4 (de) 2025-10-02
US20200005442A1 (en) 2020-01-02
US11238575B2 (en) 2022-02-01

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