DE102018210606B4 - Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors - Google Patents

Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors

Info

Publication number
DE102018210606B4
DE102018210606B4 DE102018210606.1A DE102018210606A DE102018210606B4 DE 102018210606 B4 DE102018210606 B4 DE 102018210606B4 DE 102018210606 A DE102018210606 A DE 102018210606A DE 102018210606 B4 DE102018210606 B4 DE 102018210606B4
Authority
DE
Germany
Prior art keywords
correction
signal
image
wide
correction factor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE102018210606.1A
Other languages
German (de)
English (en)
Other versions
DE102018210606A1 (de
Inventor
Viktor Drescher
Nils Langholz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carl Zeiss Microscopy GmbH
Original Assignee
Carl Zeiss Microscopy GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss Microscopy GmbH filed Critical Carl Zeiss Microscopy GmbH
Priority to DE102018210606.1A priority Critical patent/DE102018210606B4/de
Priority to US16/445,802 priority patent/US11238575B2/en
Priority to CN201910558190.1A priority patent/CN110658617B/zh
Priority to JP2019119202A priority patent/JP7254643B2/ja
Publication of DE102018210606A1 publication Critical patent/DE102018210606A1/de
Application granted granted Critical
Publication of DE102018210606B4 publication Critical patent/DE102018210606B4/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration using two or more images, e.g. averaging or subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/95—Computational photography systems, e.g. light-field imaging systems
    • H04N23/951—Computational photography systems, e.g. light-field imaging systems by using two or more images to influence resolution, frame rate or aspect ratio
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N5/00—Details of television systems
    • H04N5/30—Transforming light or analogous information into electric information
    • H04N5/32—Transforming X-rays
    • H04N5/3205—Transforming X-rays using subtraction imaging techniques
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10148—Varying focus
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20216—Image averaging
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction

Landscapes

  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Computing Systems (AREA)
  • Microscoopes, Condenser (AREA)
DE102018210606.1A 2018-06-28 2018-06-28 Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors Active DE102018210606B4 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE102018210606.1A DE102018210606B4 (de) 2018-06-28 2018-06-28 Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors
US16/445,802 US11238575B2 (en) 2018-06-28 2019-06-19 Microscopic imaging method using a correction factor
CN201910558190.1A CN110658617B (zh) 2018-06-28 2019-06-25 使用校正因子的显微成像方法
JP2019119202A JP7254643B2 (ja) 2018-06-28 2019-06-27 補正係数を用いた顕微鏡画像化法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102018210606.1A DE102018210606B4 (de) 2018-06-28 2018-06-28 Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors

Publications (2)

Publication Number Publication Date
DE102018210606A1 DE102018210606A1 (de) 2020-01-02
DE102018210606B4 true DE102018210606B4 (de) 2025-10-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE102018210606.1A Active DE102018210606B4 (de) 2018-06-28 2018-06-28 Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors

Country Status (4)

Country Link
US (1) US11238575B2 (enExample)
JP (1) JP7254643B2 (enExample)
CN (1) CN110658617B (enExample)
DE (1) DE102018210606B4 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102023102991B3 (de) * 2023-02-08 2024-02-01 Till I.D. Gmbh Verfahren zur Generierung mikroskopischer Schichtaufnahmen 3-dimensionaler fluoreszierender Objekte sowie Vorrichtung, Computerprogramm und computerlesbares Speichermedium

Citations (3)

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WO1997031282A1 (en) * 1996-02-22 1997-08-28 Isis Innovation Limited Confocal microscope
DE10014331A1 (de) * 1999-03-23 2000-10-05 Olympus Optical Co Konfokales Mikroskop
DE102014004249A1 (de) * 2014-03-24 2015-09-24 Carl Zeiss Microscopy Gmbh Konfokales Mikroskop mit Aperturkorrelation

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US5923430A (en) * 1993-06-17 1999-07-13 Ultrapointe Corporation Method for characterizing defects on semiconductor wafers
US5923465A (en) * 1994-10-28 1999-07-13 Marc J. Byrd System for scanning confocal image reconstruction from coherent recordings
CN100356163C (zh) * 1996-08-16 2007-12-19 Ge保健尼亚加拉公司 用于分析井板、凝胶和斑点的数字成像系统
DE19824460A1 (de) * 1998-05-30 1999-12-02 Zeiss Carl Jena Gmbh Anordnung und Verfahren zur mikroskopischen Erzeugung von Objektbildern
GB9901365D0 (en) * 1999-01-22 1999-03-10 Isis Innovations Ltd Confocal microscopy apparatus and method
JP2000275540A (ja) * 1999-03-24 2000-10-06 Olympus Optical Co Ltd 共焦点顕微鏡
US6341035B1 (en) * 1999-07-09 2002-01-22 Olympus Optical Co., Ltd. Confocal microscope
GB2401431B (en) * 2003-04-02 2005-04-27 Amersham Biosciences Uk Ltd Method of, and computer software for, classification of cells into subpopulations
US7170675B2 (en) * 2004-05-19 2007-01-30 Celloptic, Inc. Method and system for wide-field multi-photon microscopy having a confocal excitation plane
US8542899B2 (en) * 2006-11-30 2013-09-24 Definiens Ag Automatic image analysis and quantification for fluorescence in situ hybridization
GB201007055D0 (en) * 2010-04-28 2010-06-09 Vib Vzw Method and apparatus for the imaging of a labelled sample
US10006922B2 (en) * 2011-12-22 2018-06-26 Massachusetts Institute Of Technology Raman spectroscopy for detection of glycated analytes
WO2013109957A1 (en) * 2012-01-18 2013-07-25 University Of Utah Research Foundation Devices and systems for fluorescence imaging of tissue
US9449377B2 (en) * 2012-10-09 2016-09-20 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Imaging methods and computer-readable media
DE102013218795A1 (de) * 2013-09-19 2015-03-19 Carl Zeiss Microscopy Gmbh Laserscanningmikroskop und Verfahren zur Korrektur von Abbildungsfehlern insbesondere in der hochauflösenden Scanning-Mikroskopie
CN103926228B (zh) * 2014-04-28 2016-03-02 江苏天宁光子科技有限公司 一种激光扫描共焦荧光显微内窥成像系统
DE102016103736A1 (de) * 2016-03-02 2017-09-07 Carl Zeiss Microscopy Gmbh Verfahren zur Bestimmung einer Höhenlage eines Objekts
US10129448B2 (en) * 2016-07-20 2018-11-13 Dental Imaging Technologies Corporation Optical coherence tomography imaging system
CN107228846B (zh) * 2017-05-10 2019-09-17 暨南大学 基于离轴光束焦面共轭的荧光成像光切片方法和装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997031282A1 (en) * 1996-02-22 1997-08-28 Isis Innovation Limited Confocal microscope
DE10014331A1 (de) * 1999-03-23 2000-10-05 Olympus Optical Co Konfokales Mikroskop
DE102014004249A1 (de) * 2014-03-24 2015-09-24 Carl Zeiss Microscopy Gmbh Konfokales Mikroskop mit Aperturkorrelation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NEIL, M. A. A. ; WILSON, T. ; JUŠKAITIS, R.: A light efficient optically sectioning microscope. In: Journal of Microscopy, Vol. 189, 1998, No. 2, S. 114-117. - ISSN 0368-3974 (P); 1365-2818 (E). DOI: https://doi.org/10.1046/j.1365-2818.1998.00317.x. URL: https://onlinelibrary.wiley.com/doi/epdf/10.1046/j.1365-2818.1998.00317.x [abgerufen am 2018-11-28] *
WILSON, T. [u.a.]: Confocal microscopy by aperture correlation. In: Optics Letters (OL), Vol. 21, 1996, No. 23, S. 1879-1881. - ISSN 1539-4794 (E); 0146-9592 (P). DOI: https://doi.org/10.1364/OL.21.001879. URL: https://www.osapublishing.org/ol/viewmedia.cfm?uri=ol-21-23-1879&seq=0 [abgerufen am 2018-11-28] *

Also Published As

Publication number Publication date
JP7254643B2 (ja) 2023-04-10
JP2020003793A (ja) 2020-01-09
DE102018210606A1 (de) 2020-01-02
CN110658617A (zh) 2020-01-07
US20200005442A1 (en) 2020-01-02
CN110658617B (zh) 2023-10-20
US11238575B2 (en) 2022-02-01

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