CN110638857A - Preparation method of fructus psoraleae total glycoside extract - Google Patents
Preparation method of fructus psoraleae total glycoside extract Download PDFInfo
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Abstract
The invention relates to a preparation method of a malaytea scurfpea fruit total glycoside extract, which comprises the steps of putting malaytea scurfpea fruit medicinal materials into a vacuum freezer for vacuum freezing for 10-12 h; soaking fructus Psoraleae in water extraction device for 2-3 hr after vacuum freezing; then centrifugal separation is carried out, and the separated liquid enters a hydrolysis device after being heated and pressurized and is hydrolyzed by acid added into the hydrolysis device; the hydrolyzed hydrolysate enters a water washing device, is absorbed by macroporous resin in the water washing device, is eluted by water and ethanol, and the eluted liquid enters a reduced pressure concentration instrument for reduced pressure concentration; cooling and drying the liquid subjected to vacuum concentration by a vacuum concentration instrument to obtain a malaytea scurfpea fruit total glycoside extract; the wall of the fructus psoraleae can be effectively damaged by freezing treatment, the extraction efficiency is ensured, different instruments are adopted for effective extraction in the extraction process, the industrialized extraction can be ensured, and the extract of the fructus psoraleae can be widely used.
Description
Technical Field
The invention relates to the technical field of medicine extraction, in particular to a preparation method of a malaytea scurfpea fruit total glycoside extract.
Background
At present, Chinese herbal medicines have certain markets in the global range, along with the increase of the awareness level of people on health requirements and the aging of population, the sub-health state, people feel more thirsty and return to nature, and the natural plant medicines are used for treating and preventing the problems which cannot be solved by chemosynthetic medicines, so that the application of the natural plant medicines is beyond the background of the original national traditional culture. The search for low-cost and low-side-effect drugs from natural drugs is a pursuit target of various national medical and pharmaceutical enterprises in the world.
The fructus Psoraleae is fruit of Psoralea corylifolia (Psoralea corylifolia L) of Leguminosae, is warm in nature and pungent in flavor, and has effects of invigorating kidney and tonifying yang. It can be used for treating kidney essence deficiency cold diarrhea, frequent micturition, sexual impotence, lumbago, knee pain, asthenic cold, cough, asthma, and vitiligo. Modern day
The research shows that the fructus psoraleae has the physiological activities of expanding blood vessels, increasing myocardial contractility, resisting bacteria, having the female hormone-like effect, treating leucoderma and the like. Fructus psoraleae contains various active ingredients, and glycosides represented by psoralen and isopsoralen are one of the important active ingredients, but no reports of psoralen glycosides are available in the literature and patents at present.
In order to extract the medicinal components in the fructus psoraleae, Chinese patent CN101549010B discloses a preparation method and application of a fructus psoraleae total glycoside extract, which can effectively extract the medicaments in the fructus psoraleae, but in the extraction process, the adopted method is relatively complex, can not carry out a large amount of extraction, can only carry out extraction in a laboratory, and can not carry out extraction in a mass production mode,
disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a preparation method of a malaytea scurfpea fruit total glycoside extract, which can realize large-scale extraction of medicines in malaytea scurfpea fruit.
In order to achieve the above purpose, the invention adopts the following technical scheme, a preparation method of a malaytea scurfpea fruit total glycoside extract,
the method comprises the following steps: putting fructus Psoraleae into a vacuum freezer, and vacuum freezing for 10-12 hr;
step two: soaking fructus Psoraleae in water extraction device for 2-3 hr after vacuum freezing; then the water extraction device carries out centrifugal separation, and the separated liquid enters the hydrolysis device after being heated and pressurized and is hydrolyzed for 0.5h-1h with acid added in the hydrolysis device; the hydrolysis temperature is 80-95 degrees;
step three: the hydrolyzed hydrolysate enters a water washing device, is adsorbed by macroporous resin in the water washing device, and the adsorbed liquid is discharged and enters a waste liquid collecting tank for collection; the macroporous resin absorbing the hydrolysate is firstly cleaned by water entering a water washing device, and the cleaned water is discharged and enters a wastewater collection tank for collection; eluting the washed macroporous resin by ethanol in a water washing device, and concentrating the eluted liquid in a reduced pressure concentration instrument under reduced pressure;
step four: and cooling and drying the liquid subjected to vacuum concentration by a vacuum concentration instrument to obtain the psoralea corylifolia total glycoside extract.
Further, the water extraction device is communicated with the hydrolysis device through an extract pipeline, a first electromagnetic valve is arranged on the extract pipeline, the hydrolysis device is communicated with the water washing device through an extract pipeline, a hydrolysate pump is arranged on the hydrolysate pipeline, the water washing device is communicated with the waste liquid collecting pool, the reduced pressure concentrator and the waste water collecting box through a waste liquid pipeline, an ethanol eluent pipeline and a waste water pipeline respectively, and a second electromagnetic valve, a third electromagnetic valve and a fourth electromagnetic valve are arranged on the waste liquid pipeline, the ethanol eluent pipeline and the waste water pipeline respectively;
the water inlet of the water washing device is communicated with a water tank and an ethanol storage tank through a water washing pipeline and an ethanol washing pipeline respectively, a water washing pump for pumping water in the water tank into the water washing device is arranged on the water washing pipeline, and an ethanol eluting pump for pumping ethanol in the ethanol storage tank into the water washing device is arranged on the ethanol washing pipeline.
Further, the water extraction device comprises an outer shell, a top cover buckled at the top of the outer shell and a throwing barrel arranged in the outer shell, wherein the outer wall of the throwing barrel and the inner wall of the outer shell form an annular cavity, the throwing barrel is provided with a plurality of filtering holes for communicating the inner cavity of the throwing barrel with the annular cavity, and one-way valves are arranged in the filtering holes;
a centrifugal motor box is fixedly arranged at the bottom of the outer shell, a centrifugal motor is fixedly arranged in the centrifugal motor box, and a rotating shaft of the centrifugal motor penetrates through the top of the centrifugal motor box and is fixedly connected with a connecting disc fixedly arranged at the center of the bottom of the throwing barrel; the lower part of the throwing barrel is sleeved with an annular bearing ring, the outer ring of the annular bearing ring is fixedly connected with the outer shell, and the inner ring of the annular bearing ring is in sealing contact with the outer wall of the throwing barrel;
a sealing plug for sealing a barrel opening of the barrel throwing is arranged at the center of the lower end part of the top cover, and the sealing plug is rotatably connected with the top cover; the lower end part of the top cover is also provided with a pressure ring which is sleeved outside the sealing plug and has a gap with the outer wall of the sealing plug, the pressure ring is fixedly connected with a telescopic section of a telescopic electric cylinder embedded in the top cover, the telescopic electric cylinder pushes the pressure ring into the annular cavity to move up and down in the annular cavity, and the pressure ring applies pressure to the centrifugal liquid entering the annular cavity;
a plurality of heating rods vertical to the annular bearing ring are fixedly arranged on the annular bearing ring, the top of each heating rod penetrates through the compression ring to be in contact communication with an electric contact arranged at the bottom of the top cover, and the electric contact is communicated with an external power supply; the heating rod is connected with the pressure ring through a sealing ring, and the heating rod is in smooth sealing contact with the sealing ring;
the lower part of the outer shell is provided with an extracting solution outlet communicated with an extracting solution pipeline, and the extracting solution outlet is communicated with the annular cavity.
Furthermore, the hydrolysis device comprises a hydrolysis box body, an air outlet arranged at the top of the hydrolysis box body, an extracting solution inlet arranged at the upper part of the hydrolysis box body and communicated with an extracting solution pipeline, and a hydrolyzing solution outlet arranged at the bottom of the hydrolysis box body and communicated with a hydrolyzing solution pipeline, wherein multiple layers of impact plates with gaps are fixedly arranged at the upper part in the hydrolysis box body and positioned above the extracting solution inlet, multiple through holes are arranged on each layer of impact plate, and the through holes on two adjacent layers of impact plates are staggered;
the fixed sour bin that is equipped with in one side of the box of hydrolysising, this sour bin and the internal shower nozzle of hydrolysising pass through sour entering pipeline intercommunication, and this sour entering pipeline is equipped with the sour entering pump of going into the shower nozzle with the sour pump in the sour bin, the shower nozzle is located the below of extract entry.
Furthermore, the water washing device comprises a water washing box body, a water inlet pipeline and a hydrolysate inlet communicated with the hydrolysate pipeline are arranged at the upper part of the water washing box body, a tee joint is connected to the water inlet pipeline, and two inlets of the tee joint are respectively communicated with the water washing pipeline and the ethanol washing pipeline; the bottom of the washing box body is provided with a liquid outlet which is respectively communicated with a waste liquid pipeline, an ethanol eluent pipeline and a waste water pipeline through a four-way joint;
a roller for placing macroporous resin is arranged in the lower part of the washing box body, a plurality of meshes with the diameter smaller than that of the macroporous resin are arranged on the roller, and two ends of the roller are carried on annular tables arranged on two sides of the washing box body; a roller motor is fixedly arranged on the outer wall of one side of the washing box body, and a rotating shaft of the fixed motor penetrates through the washing box body to be fixedly connected with one end part of the roller; the other end of the roller is in an opening shape, and a sealing door is arranged on the side wall of the washing box body corresponding to the roller at the opening end.
Further, the check valve comprises a valve body arranged in the filter hole, a main channel penetrating through the valve body is arranged in the middle of the valve body, a plurality of branch channels communicated with the main channel are arranged on the inner wall of the outlet end of the main channel, a valve core used for plugging the main channel is arranged in the main channel, a mounting plate with a liquid passing hole is arranged in the position, close to the inlet end, of the main channel, a positioning rod perpendicular to the end face of the valve core is arranged on the valve core, the end portion of the positioning rod penetrates through the mounting plate and slides relative to the mounting plate, a spring is sleeved on the positioning rod between the mounting plate and the valve core, two ends of the spring are fixedly connected with the valve core and the mounting plate respectively, when the swinging barrel does not rotate, the spring is in a reset state, and the valve core plugs the.
Furthermore, a bearing is embedded in the center of the top of the sealing plug, a connecting shaft is fixedly arranged in the center of the bottom end face of the top cover, the connecting shaft is fixedly connected with an inner ring of the bearing, and a gap is formed between the upper surface of the sealing plug and the lower surface of the top cover.
Furthermore, the top of top cap is fixed and is equipped with the force (forcing) pump, and this force (forcing) pump passes through the pressurization pipeline intercommunication with the pressurization passageway that runs through to be equipped with on the clamping ring.
The small end face of the top cover is provided with a groove, the electric contact is fixedly arranged in the groove, the top of the heating rod is provided with a slot, and the electric contact is inserted into the slot to communicate the heating rod with the electric contact.
The application of the fructus Psoraleae total glycosides extract prepared by the method in medicine for treating leukemia is provided.
The invention has the beneficial effects that: the invention freezes the fructus psoraleae before extracting, can effectively destroy the wall of the fructus psoraleae, ensures that the extracted medicine is more thorough in the later extraction process, ensures the extraction efficiency, adopts different instruments to effectively extract in the extraction process, can ensure the realization of industrialized extraction, improves the extraction yield and the extraction rate, and enables the extract of the fructus psoraleae to be widely used.
Drawings
FIG. 1 is a schematic diagram of the structure of the instrument connection of the present invention;
FIG. 2 is a schematic view of the structure of a water extracting apparatus according to the present invention
FIG. 3 is a schematic view of the structure of a hydrolysis apparatus according to the present invention;
FIG. 4 is a schematic view showing the structure of a water washing apparatus according to the present invention;
FIG. 5 is an enlarged view of the portion A of FIG. 2 according to the present invention;
FIG. 6 is an enlarged view of the structure of the portion B in FIG. 2 according to the present invention;
fig. 7 is a schematic view showing a structure in which the heating rod of fig. 2 is butt-jointed at the top thereof according to the present invention.
Detailed Description
The invention is described in detail below with reference to the figures and examples.
In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature; in the description of the present invention, "a plurality" means two or more unless otherwise specified.
Example 1
A method for preparing fructus Psoraleae total glycosides extract,
the method comprises the following steps: putting fructus Psoraleae into a vacuum freezer, and vacuum freezing for 10-12 hr; the method specifically comprises the step of putting the fructus psoraleae particles into a vacuum freezer for freezing, wherein cell walls in the frozen fructus psoraleae particles are effectively damaged, so that the extracts in the fructus psoraleae particles can be conveniently and effectively extracted in the later period, the specific freezing time is 11H, the cell walls of the fructus psoraleae particles can be effectively damaged, and the temperature for cooling is-50 ℃.
Step two: soaking fructus Psoraleae in water extraction device 1 for 2-3 hr after vacuum freezing; then the water extraction device 1 carries out centrifugal separation, and the separated liquid enters the hydrolysis device 2 and the acid added into the hydrolysis device 2 for hydrolysis for 0.5h-1h after being heated and pressurized; the hydrolysis temperature is 80-95 ℃; the frozen fructus psoraleae particles with the broken cell walls are placed into a water extraction device 1 for soaking, because the cell walls of the frozen fructus psoraleae particles are effectively broken, soaking in water at 45-60 deg.C for 3H, allowing water to enter fructus Psoraleae granule and extract therein for sufficiently combining, then the water extraction device 1 carries out centrifugal motion to separate the extract after water extraction from the fructus psoraleae granules under the action of centrifugal force, then heating the water extract, pumping the heated water extract into the hydrolysis device 2 at 90-100 deg.C, and hydrolyzing with acid (hydrochloric acid, sulfuric acid, nitric acid or phosphorus) in the hydrolysis device 2 at 80-95 deg.C;
step three: the hydrolyzed hydrolysate enters a water washing device 3, is absorbed by macroporous resin in the water washing device 3, and the liquid after absorption is discharged and enters a waste liquid collecting tank 10 for collection; the macroporous resin adsorbing the hydrolysate is firstly cleaned by water entering the water washing device 3, and the cleaned water is discharged and enters the wastewater collection box 12 for collection; eluting the washed macroporous resin by ethanol entering the water washing device 3, and carrying out reduced pressure concentration on the eluted liquid entering a reduced pressure concentration instrument 11; the vacuum concentrator is a conventional vacuum concentrator at present, and the structure of the vacuum concentrator is not described in detail;
step four: cooling and drying the liquid after the concentration under reduced pressure by a reduced pressure concentration instrument 11 to obtain the total glycoside extract of the bone tonifying fat.
According to the method, different devices are adopted for continuous extraction in the extraction process, so that the industrial extraction is guaranteed, the large-batch extraction can be guaranteed, meanwhile, in the extraction process, the cell walls of the fructus psoraleae particles can be guaranteed to be effectively damaged after being frozen, the water extraction rate can be guaranteed, meanwhile, centrifugation is adopted for separation in the extraction process, the fructus psoraleae particles and the water extract can be guaranteed to be effectively separated, the extract in the fructus psoraleae particles can be fully extracted, meanwhile, after the extraction is completed, the waste water in the extraction process is effectively collected, and the pollution of waste water discharge to the environment is avoided.
The device used in the extraction process is connected according to the mode of fig. 1, specifically, the water extraction device 1 is communicated with the hydrolysis device 2 through an extract pipeline 4, a first electromagnetic valve 6 is arranged on the extract pipeline 4, the hydrolysis device 2 is communicated with the water washing device 3 through a hydrolysate pipeline 5, a hydrolysate pump 7 is arranged on the hydrolysate pipeline 5, the water washing device 3 is communicated with the waste liquid collecting tank 10, the reduced pressure concentrator 11 and the wastewater collecting tank 12 through a waste liquid pipeline 15, an ethanol eluent pipeline 16 and a wastewater pipeline 17 respectively, and the waste liquid pipeline 15, the ethanol eluent pipeline 16 and the wastewater pipeline 17 are provided with a second electromagnetic valve 20, a third electromagnetic valve 21 and a fourth electromagnetic valve 22 respectively; putting the vacuum-frozen fructus psoraleae particles into a water extraction device, soaking and centrifuging, opening a first electromagnetic valve 6 to allow an extracting solution to enter a hydrolysis device 2 through an extracting solution pipeline 4 for hydrolysis, and pumping the hydrolyzed liquid into a water washing device 3 through a hydrolytic liquid pump 7 for washing;
in the water washing process, in order to realize effective water washing sequentially, as shown in fig. 4, a water tank 8 and an ethanol storage tank 9 are respectively communicated with a water inlet of the water washing device 3 through a water washing pipeline 13 and an ethanol washing pipeline 14, a water washing pump 18 for pumping water in the water tank 8 into the water washing device 3 is arranged on the water washing pipeline 13, and an ethanol elution pump 19 for pumping ethanol in the ethanol storage tank 9 into the water washing device 3 is arranged on the ethanol washing pipeline 14; the hydrolysis extract entering the water washing device 3 is absorbed by macroporous resin, and under the condition that the second electromagnetic valve is opened after absorption, at the moment, the third electromagnetic valve and the fourth electromagnetic valve are closed, the waste liquid enters a waste liquid collecting tank for collection, then the second electromagnetic valve is closed, the water cleaning pump 18 is opened at the moment, the water cleaning pump pumps the water in the water tank 8 into the water cleaning device to clean the adsorbed macroporous resin, after the cleaning is finished, opening the fourth electromagnetic valve, discharging the cleaned wastewater, collecting the wastewater by a wastewater collection box, closing the fourth electromagnetic valve, opening an ethanol elution pump, pumping the ethanol into a water cleaning device, and (3) eluting the macroporous resin, opening a third electromagnetic valve after the elution is finished, allowing the eluent to enter a reduced pressure concentration instrument 11 for reduced pressure concentration treatment, and drying and cooling after the reduced pressure concentration treatment to obtain the fructus psoraleae extract.
Further, in order to ensure that soaking, centrifugal separation and heating can be effectively performed as much as possible in the water extraction process, and the efficiency of water extraction is ensured, as shown in fig. 2, the water extraction device 1 comprises an outer shell 101, a top cover 102 buckled on the top of the outer shell 101, and a throwing barrel 103 arranged in the outer shell 101, wherein the outer wall of the throwing barrel 103 and the inner wall of the outer shell 101 form an annular cavity 104, the throwing barrel 103 is provided with a plurality of filtering holes 106 for communicating the inner cavity of the throwing barrel 103 with the annular cavity 104, and a one-way valve is arranged in each filtering hole 106; the throwing barrel 103 is used for centrifugal separation, the filtering holes 106 arranged on the throwing barrel 103 can ensure that water extract can be separated from the throwing barrel in the centrifugal motion process, and the one-way valves arranged in the filtering holes 106 prevent water and water extract from entering the annular cavity 104 through the filtering holes too early in the soaking process and prevent liquid in the annular cavity 104 from entering the throwing barrel;
specifically, the check valve includes a valve body 123 disposed in the filter hole 106 as shown in fig. 6, a main channel 124 penetrating through the valve body 203 is disposed in the middle of the valve body 123, a plurality of branch channels 125 communicating with the main channel 124 are disposed on the inner wall of the outlet end of the main channel 124, a valve core 126 for blocking the main channel 124 is disposed in the main channel 124, a mounting plate 127 having a liquid passing hole 128 is disposed in the main channel 124 near the inlet end, a positioning rod 130 perpendicular to the end surface of the valve core 126 is disposed on the valve core 126, the end of the positioning rod 130 passes through the mounting plate 127 and slides relative to the mounting plate 127, a spring 129 is sleeved on the positioning rod 130 between the mounting plate 127 and the valve core 126, two ends of the spring 129 are fixedly connected to the valve core 126 and the mounting plate 127, and when the spin basket 103 is not rotated, the spring 129 is in a restoring state, the valve element 126 seals off the main channel 124. The valve body is fixedly arranged in the filter hole, the connection tightness needs to be ensured, the valve core is used for plugging the main channel, particularly when the valve core is positioned close to the inlet end of the main channel, the main channel is effectively plugged, and when the valve core is positioned in the branch channel, the branch channel is communicated with the main channel to communicate the throwing barrel with the annular cavity; when the centrifugal water extraction device works, the spring is under the self tension (under the restoring tension) when the throwing barrel does not do centrifugal motion, the valve core is positioned at the inlet of the main channel to block the main channel, after soaking is finished, the throwing barrel does centrifugal motion, the valve core moves towards the outlet of the main channel under the action of the centrifugal force and the pressure of water extraction, when the valve core moves to the position of the branch channel, the branch channel is communicated with the main channel, the water extraction enters the annular cavity, the water extraction can not enter the annular cavity in the soaking process, and can fully enter the annular cavity during the centrifugal motion; the locating rod 130 is used for locating the whole valve core, avoids sliding in the moving process, and when centrifugal motion is completed, a spring of a stay cord needs to be restored to pull the valve core to move towards an inlet of the main channel, so that the main channel is blocked from a new position, and liquid in the annular cavity is prevented from entering the throwing barrel.
Specifically, when the swing barrel performs centrifugal motion, a driving mechanism is adopted, wherein a centrifugal motor box 108 is fixedly arranged at the bottom of the outer shell 101, a centrifugal motor 107 is fixedly arranged in the centrifugal motor box 108, and a rotating shaft of the centrifugal motor 107 penetrates through the top of the centrifugal motor box 108 to be fixedly connected with a connecting disc 109 fixedly arranged at the center position of the bottom of the swing barrel 103; an annular bearing ring 110 is sleeved at the lower part of the throwing barrel 103, the outer ring of the annular bearing ring 110 is fixedly connected with the outer shell 101, and the inner ring of the annular bearing ring 110 is in sealing contact with the outer wall of the throwing barrel 103; centrifugal motor case 108 right centrifugal motor carry out effectual protection, be connected through connection pad and centrifugal motor's pivot, convenient connection, and the more reliable safety of connection, in order to guarantee get rid of the bucket can carry out stable rotation under centrifugal motor's drive, the liquid that the annular carried ring 110 simultaneously can not enter into the lower part of shell body 101, causes the influence to the motor get rid of bucket 103 lower part and be equipped with round annular groove 117, be equipped with the sealed annular picture peg 118 that inserts in this annular groove 117 of round on the periphery that the annular carried ring 110, when guaranteeing to carry out sealing connection, get rid of the bucket and can carry out the motion by relative annular carrier ring.
Further, in order to ensure that the top cover can seal the whole device and simultaneously cannot influence the movement of the swinging barrel, a sealing plug 105 for sealing the barrel opening of the swinging barrel 103 is arranged at the center of the lower end part of the top cover 102, and the sealing plug 105 is rotatably connected with the top cover 102; specifically, as shown in fig. 5, a bearing 122 is embedded in the center of the top of the sealing plug 105, a connecting shaft 121 is fixedly disposed in the center of the bottom end surface of the top cover 102, the connecting shaft 121 is fixedly connected with an inner ring of the bearing 122, and a gap is formed between the upper surface of the sealing plug 105 and the lower surface of the top cover 102; the effectual sealing plug of having guaranteed can seal the barrel of getting rid of, can also rotate by relative top cap.
The lower end part of the top cover 102 is also provided with a pressing ring 111, the pressing ring 111 is sleeved outside the sealing plug 105 and has a gap with the outer wall of the sealing plug 105, the pressing ring 111 is fixedly connected with a telescopic section of a telescopic electric cylinder 113 embedded in the top cover 102, the telescopic electric cylinder 113 pushes the pressing ring 111 into the annular cavity 104 to move up and down in the annular cavity 104, and the centrifugal liquid entering the annular cavity 104 is pressed; the clamping ring is used for sealing the annular cavity, specifically is through flexible electric cylinder drive carry out effectual sealing to the annular cavity, simultaneously after accomplishing centrifugal motion, need exert pressure with the extract in the annular cavity, impress in hydrolysis unit 2, flexible electric cylinder extend, promote the clamp plate and descend, exert pressure to the extract, impress the extract in hydrolysis unit, in order to satisfy its pressure the fixed force (forcing) pump 116 that is equipped with in top cap 102's top, this force (forcing) pump 116 with run through the pressure channel 115 that is equipped with on clamping ring 111 and pass through the pipeline intercommunication that adds pressure, exert pressure through the force (forcing) pump, increase its pressure, guarantee the value that the pressure of the liquid that enters into hydrolysis unit 2 can set for, can carry out abundant hydrolysis.
In order to ensure that the extracting solution is effectively heated, a plurality of heating rods 114 (which are electric heating rods and can generate heat after being electrically connected with the heating rods) perpendicular to the annular bearing ring 110 are fixedly arranged on the annular bearing ring 110 (the heating rods belong to the common general knowledge in the technical field and detailed description is omitted here), the top of each heating rod 114 penetrates through the press ring 111 to be in contact communication with an electric contact 119 arranged at the bottom of the top cover 102, and the electric contact 119 is communicated with an external power supply; the heating rod 114 is connected with the pressure ring 111 through a sealing ring 112, and the heating rod 114 is in smooth sealing contact with the sealing ring 112; in order to ensure the stability of the connection of the heating rod and to perform stable heating, as shown in fig. 7, a groove 1021 is provided on the small end surface of the top cover 102, the electrical contact 119 is fixedly provided in the groove 1021, a slot 1041 is provided on the top of the heating rod 114, the electrical contact 119 is inserted into the slot 1041 to communicate the heating rod 114 with the electrical contact 119, the plug-in connection is adopted to make the connection stable and the plug-in connection convenient,
the lower part of the outer casing 101 is provided with an extract outlet 120 in line communication with the extract line 4, the extract outlet 120 being in communication with the annular chamber 104. The soaked, centrifuged and heated water extract enters the extract line 4 through the extract outlet 120 under pressure and then enters the hydrolysis apparatus, completing the water extraction process.
In the hydrolysis process, in order to ensure that the hydrolysis process is performed quickly and completely, as shown in fig. 3, the hydrolysis apparatus 2 includes a hydrolysis tank 201, an air outlet 204 disposed at the top of the hydrolysis tank 201, an extract inlet 202 disposed at the upper portion of the hydrolysis tank 201 and communicated with an extract pipeline 4, and an extract outlet 203 disposed at the bottom of the hydrolysis tank 201 and communicated with an extract pipeline 5, wherein a plurality of layers of impact plates 205 with gaps are fixedly disposed at the upper portion of the hydrolysis tank 201, the plurality of layers of impact plates 205 are disposed above the extract inlet 202, each layer of impact plate 205 is provided with a plurality of through holes 206, and the through holes 206 on two adjacent layers of impact plates 205 are staggered; the gas outlet 204 is used for ensuring that the gas pressure in the hydrolysis tank body 201 is kept stable, and the ascending gas is subjected to gas-liquid separation through the plurality of impact plates 205 with the through holes 206, so that the phenomenon that the gas brings out liquid in the hydrolysis process to cause environmental pollution and waste of extracts is avoided;
an acid storage tank 207 is fixedly arranged on one side of the hydrolysis tank body 201, the acid storage tank 207 is communicated with a spray head 209 in the hydrolysis tank body 201 through an acid inlet pipeline 208, an acid inlet pump 210 for pumping acid in the acid storage tank 207 into the spray head 207 is arranged on the acid inlet pipeline 208, and the spray head 209 is positioned below the extracting solution inlet 202. The pressurized extracting solution enters the hydrolysis tank body 201 to form a fog shape, and can be fully combined with acid sprayed by the spray head, so that full hydrolysis is ensured, and the hydrolysis efficiency is improved.
As shown in fig. 4, the water washing device 3 includes a water washing tank 301, a water inlet pipeline 303 and a hydrolysate inlet 302 communicated with the hydrolysate pipeline 5 are provided at an upper portion of the water washing tank 301, a tee 23 is connected to the water inlet pipeline 303, and two inlets of the tee 23 are respectively communicated with the water washing pipeline 13 and the ethanol washing pipeline 14; the bottom of the water washing box body 301 is provided with a liquid outlet 304, and the liquid outlet 304 is respectively communicated with a waste liquid pipeline 15, an ethanol eluent pipeline 16 and a waste water pipeline 17 through a four-way 24; the three-way connector and the four-way connector are connected through the connecting piece, so that the uniformity of the three-way connector and the four-way connector is guaranteed;
a roller 306 for placing macroporous resin is arranged in the lower part of the water washing box body 301, a plurality of meshes 305 with the diameter smaller than that of the macroporous resin are arranged on the roller 306, and two ends of the roller 306 are arranged on annular platforms 307 arranged on two sides of the water washing box body 301; a roller motor 308 is fixedly arranged on the outer wall of one side of the washing box body 301, and a rotating shaft of the fixed motor 308 penetrates through the washing box body 301 to be fixedly connected with one end part of the roller 306; the other end of the drum 306 is open, and a sealing door 309 is provided on the side wall of the washing tank 301 corresponding to the open end drum. The macroporous resin is put into the roller 306, the hydrolysis extract entering the water washing box body 301 can be in full contact with the hydrolysis extract under the rotation of the roller for adsorption, the second electromagnetic valve is opened after the adsorption is finished, the adsorbed sub-waste liquid is discharged, then the second electromagnetic valve is closed, the water washing pump is opened, the water entering the water washing box body fully washes the macroporous resin attached with the hydrolysis extract, the rolling motor drives the rolling motor to rotate in the washing process to ensure that the washing is more thorough, the fourth electromagnetic valve is opened to discharge the washed sub-waste water after the washing is finished, the fourth electromagnetic valve is closed, the ethanol elution pump 19 is opened at the moment, the ethanol entering the washing box body is in full contact with the macroporous resin in the roller in the rotation process to realize the ethanol elution, and after the elution is finished, and opening the third electromagnetic valve, enabling the eluted liquid to enter a reduced pressure concentration instrument 11 for reduced pressure concentration treatment, drying and cooling after reduced pressure concentration to obtain the fructus psoraleae extract, and opening a sealing door 309 to replace the macroporous resin in the roller after extraction is completed.
Example two
The prepared fructus Psoraleae total glycoside extract has effects on blood WBC, HB, and PLT in the treatment process of mice with leukemia model;
purpose of the experiment: the fructus Psoraleae total glycoside extract can improve WBC, HB and PLT of mice of leukemia model;
experimental materials: capsule prepared from fructus Psoraleae total glycosides extract, and existing chemotherapy medicine; a chemotherapy device;
subject: the leukemia model mouse is half male and female, and the weight is 18-22 g;
the method comprises the following steps: breeding leukemia model mice of an experimental object under the same condition, and after breeding for two days, randomly grouping the mice in a sex-recording manner into three groups, wherein the number of the mice in each group is 10;
feeding the first group of mice with fructus Psoraleae total glycoside extract capsule and chemotherapy medicine 250 mg/kg daily for seven days;
the second group of mice injected with 0.5ml of chemotherapy drugs each day for seven consecutive days;
the third group of mice was treated with chemotherapy for 2 courses within seven days.
WBC, HB and PLT in peripheral blood images of three groups of mice are measured;
| group of | WBC(109/L) | HB(g/L) | PLT(109/L) |
| First group | 24.3 | 94 | 482 |
| Second group | 25.8 | 94 | 522 |
| Third group | 25.3 | 93 | 542 |
| Before treatment | 26.5 | 91 | 636 |
The results show that the peripheral blood images (WBC, HB and PLT) of the mice are improved to different degrees after the three groups of treatments; the bone marrow nucleated cell count of the capsule of the psoralea corylifolia total glycoside extract and the chemotherapy drug which are taken by drenching is higher than that of the single injection chemotherapy drug and the chemotherapy group, and the difference is obvious compared with the model group (P is less than 0.05). Therefore, the fructus psoraleae capsule can relieve the bone marrow suppression caused by chemotherapy, so that the bone marrow enters a recovery stage earlier, the recovery of neutrophils is obvious, and the fructus psoraleae capsule has a certain promotion effect on the hematopoiesis of the erythrocytic system. After the hematopoietic cells are damaged, the active ingredients in the fructus psoraleae capsule have the functions of repairing and synthesizing damaged DNA and increasing the activity of endonuclease, so that the normal hematopoietic system is quickly recovered, and the complications such as bleeding and infection caused by the reduction of platelets and white cells are reduced, thereby improving the curative effect of chemotherapy on leukemia.
The above embodiments are merely illustrative of the present invention, and should not be construed as limiting the scope of the present invention, and all designs identical or similar to the present invention are within the scope of the present invention.
Claims (10)
1. A method for preparing a malaytea scurfpea fruit total glycoside extract is characterized in that,
the method comprises the following steps: putting fructus Psoraleae into a vacuum freezer, and vacuum freezing for 10-12 hr;
step two: soaking the vacuum-frozen fructus Psoraleae in water extraction device (1) for 2-3 h; then the water extraction device (1) carries out centrifugal separation, and the separated liquid enters the hydrolysis device (2) and the acid added into the hydrolysis device (2) for hydrolysis for 0.5h-1h after being heated and pressurized; the hydrolysis temperature is 80-95 degrees;
step three: the hydrolyzed hydrolysate enters a water washing device (3) and is absorbed by macroporous resin in the water washing device (3), and the liquid after absorption is discharged and enters a waste liquid collecting tank (10) for collection; the macroporous resin adsorbing the hydrolysate is firstly cleaned by water entering the water washing device (3), and the cleaned water is discharged and enters the wastewater collection box (12) for collection; eluting the macroporous resin after water washing by ethanol entering a water washing device (3), and carrying out reduced pressure concentration on the eluted liquid entering a reduced pressure concentration instrument (11);
step four: the liquid after the decompression concentration by a decompression concentration instrument (11) is cooled and dried to obtain the malaytea scurfpea fruit total glycoside extract.
2. The method for preparing psoralea corylifolia total glycoside extract according to claim 1, wherein the psoralea corylifolia total glycoside extract is obtained by extracting psoralea corylifolia total glycoside, the water extraction device (1) is communicated with the hydrolysis device (2) through an extracting solution pipeline (4), and the extracting solution pipeline (4) is provided with a first electromagnetic valve (6), the hydrolysis device (2) is communicated with the water washing device (3) through a hydrolysis solution pipeline (5), the hydrolysate pipeline (5) is provided with a hydrolysate pump (7), the water washing device (3) is communicated with the waste liquid collecting tank (10), the decompression concentrator (11) and the waste water collecting tank (12) through a waste liquid pipeline (15), an ethanol eluent pipeline (16) and a waste water pipeline (17) respectively, a second electromagnetic valve (20), a third electromagnetic valve (21) and a fourth electromagnetic valve (22) are respectively arranged on the waste liquid pipeline (15), the ethanol eluent pipeline (16) and the waste water pipeline (17);
the water inlet of water washing device (3) is respectively through washing pipeline (13) and ethanol washing pipeline (14) intercommunication have water tank (8) and ethanol bin (9), be equipped with water washing pump (18) of going into water washing device (3) with the water pump in water tank (8) on washing pipeline (13), be equipped with one on ethanol washing pipeline (14) and go into ethanol elution pump (19) in water washing device (3) with the ethanol pump in ethanol bin (9).
3. The preparation method of the psoralea corylifolia total glycoside extract, according to claim 2, wherein the water extraction device (1) comprises an outer shell (101), a top cover (102) fastened on the top of the outer shell (101), and a throwing barrel (103) arranged in the outer shell (101), wherein the outer wall of the throwing barrel (103) and the inner wall of the outer shell (101) form an annular cavity (104), the throwing barrel (103) is provided with a plurality of filtering holes (106) for communicating the inner cavity of the throwing barrel (103) with the annular cavity (104), and the filtering holes (106) are internally provided with one-way valves;
a centrifugal motor box (108) is fixedly arranged at the bottom of the outer shell (101), a centrifugal motor (107) is fixedly arranged in the centrifugal motor box (108), and a rotating shaft of the centrifugal motor (107) penetrates through the top of the centrifugal motor box (108) to be fixedly connected with a connecting disc (109) fixedly arranged at the center of the bottom of the throwing barrel (103); an annular bearing ring (110) is sleeved at the lower part of the throwing barrel (103), the outer ring of the annular bearing ring (110) is fixedly connected with the outer shell (101), and the inner ring of the annular bearing ring (110) is in sealing contact with the outer wall of the throwing barrel (103);
a sealing plug (105) for sealing the barrel opening of the swinging barrel (103) is arranged at the central position of the lower end part of the top cover (102), and the sealing plug (105) is rotatably connected with the top cover (102); the lower end part of the top cover (102) is also provided with a pressing ring (111), the pressing ring (111) is sleeved outside the sealing plug (105) and has a gap with the outer wall of the sealing plug (105), the pressing ring (111) is fixedly connected with a telescopic section of a telescopic electric cylinder (113) embedded in the top cover (102), the telescopic electric cylinder (113) pushes the pressing ring (111) into the annular cavity (104) to move up and down in the annular cavity (104), and the centrifugal liquid entering the annular cavity (104) is pressed;
a plurality of heating rods (114) perpendicular to the annular bearing ring (110) are fixedly arranged on the annular bearing ring (110), the top of each heating rod (114) penetrates through the compression ring (111) to be in contact communication with an electric contact (119) arranged at the bottom of the top cover (102), and the electric contact (119) is communicated with an external power supply; the heating rod (114) is connected with the pressure ring (111) through a sealing ring (112), and the heating rod (114) is in smooth sealing contact with the sealing ring (112);
the lower part of the outer shell (101) is provided with an extracting solution outlet (120) which is communicated with the extracting solution pipeline (4), and the extracting solution outlet (120) is communicated with the annular cavity (104).
4. The method for preparing the psoralea corylifolia total glycoside extract according to claim 2 or 3, wherein the hydrolysis device (2) comprises a hydrolysis box body (201), an air outlet (204) arranged at the top of the hydrolysis box body (201), an extract inlet (202) arranged at the upper part of the hydrolysis box body (201) and communicated with an extract pipeline (4), and an extract outlet (203) arranged at the bottom of the hydrolysis box body (201) and communicated with an extract pipeline (5), wherein a plurality of layers of impact plates (205) with gaps are fixedly arranged at the upper part in the hydrolysis box body (201), the plurality of layers of impact plates (205) are positioned above the extract inlet (202), a plurality of through holes (206) are arranged on each layer of impact plate (205), and the through holes (206) on two adjacent layers of impact plates (205) are staggered;
an acid storage tank (207) is fixedly arranged on one side of the hydrolysis tank body (201), the acid storage tank (207) is communicated with a spray head (209) in the hydrolysis tank body (201) through an acid inlet pipeline (208), an acid inlet pump (210) for pumping acid in the acid storage tank (207) into the spray head (207) is arranged on the acid inlet pipeline (208), and the spray head (209) is positioned below the extracting solution inlet (202).
5. The method for preparing the psoralea corylifolia total glycoside extract according to claim 2, wherein the water washing device (3) comprises a water washing box body (301), a water inlet pipeline (303) and a hydrolysate inlet (302) communicated with the hydrolysate pipeline (5) are arranged at the upper part of the water washing box body (301), a tee joint (23) is connected to the water inlet pipeline (303), and two inlets of the tee joint (23) are respectively communicated with the water washing pipeline (13) and the ethanol washing pipeline (14); a liquid outlet (304) is formed in the bottom of the water washing box body (301), and the liquid outlet (304) is respectively communicated with a waste liquid pipeline (15), an ethanol eluent pipeline (16) and a waste water pipeline (17) through a cross joint (24);
a roller (306) for placing macroporous resin is arranged in the lower part of the water washing box body (301), a plurality of meshes (305) with the diameter smaller than that of the macroporous resin are arranged on the roller (306), and two ends of the roller (306) are carried on annular platforms (307) arranged on two sides of the water washing box body (301); a roller motor (308) is fixedly arranged on the outer wall of one side of the washing box body (301), and a rotating shaft of the fixed motor (308) penetrates through the washing box body (301) to be fixedly connected with one end part of a roller (306); the other end of the roller (306) is open, and a sealing door (309) is arranged on the side wall of the washing box body (301) corresponding to the open end roller.
6. The preparation method of psoralea corylifolia total glycoside extract, according to claim 3, wherein the one-way valve comprises a valve body (123) disposed in the filtering hole (106), the middle of the valve body (123) is provided with a main channel (124) penetrating through the valve body (203), the inner wall of the outlet end of the main channel (124) is provided with a plurality of branch channels (125) communicated with the main channel (124), the main channel (124) is provided with a valve core (126) for plugging the main channel (124), the main channel (124) is provided with a mounting plate (127) having a liquid passing hole (128) near the inlet end, the valve core (126) is provided with a positioning rod (130) perpendicular to the end surface of the valve core (126), the end of the positioning rod (130) passes through the mounting plate (127) and slides relative to the mounting plate (127), the positioning rod (130) disposed between the mounting plate (127) and the valve core (126) is sleeved with a spring (129), two ends of the spring (129) are respectively fixedly connected with the valve core (126) and the mounting plate (127), and when the swinging barrel (103) does not rotate, the spring (129) is in a reset state, and the valve core (126) seals the main channel (124).
7. The method for preparing the psoralea corylifolia total glycoside extract according to claim 3, wherein a bearing (122) is embedded in the center of the top of the sealing plug (105), a connecting shaft (121) is fixedly arranged in the center of the bottom end surface of the top cover (102), the connecting shaft (121) is fixedly connected with an inner ring of the bearing (122), and a gap is formed between the upper surface of the sealing plug (105) and the lower surface of the top cover (102).
8. The method for preparing fructus psoraleae total glycoside extract according to claim 3, wherein a pressure pump (116) is fixedly arranged on the top of the top cover (102), and the pressure pump (116) is communicated with a pressure channel (115) which is arranged on the pressure ring (111) in a penetrating way through a pressure pipeline.
9. The method for preparing the psoralea corylifolia total glycoside extract according to claim 3, wherein a groove (1021) is arranged on the small end face of the top cover (102), the electrical contact (119) is fixedly arranged in the groove (1021), a slot (1041) is arranged on the top of the heating rod (114), and the electrical contact (119) is inserted into the slot (1041) to communicate the heating rod (114) with the electrical contact (119).
10. The use of the extract of total glycosides of psoralea corylifolia as prepared by the process of claim 1 in a medicament for the treatment of leukemia.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111280448A (en) * | 2020-02-14 | 2020-06-16 | 山东博奥克生物科技有限公司 | Sea-buckthorn and cistanche slice and processing production equipment thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101549010A (en) * | 2009-05-14 | 2009-10-07 | 浙江大学 | A preparing method and application of malaytea scurfpea fruit total glycosides extract |
| CN105833428A (en) * | 2016-05-29 | 2016-08-10 | 洋县新健康医院 | Warming and smoothing medicine bag and making method thereof |
| CN105942539A (en) * | 2016-05-30 | 2016-09-21 | 广西华佳丝绸有限公司 | Method for producing dietary fibers by using mulberry twigs and kudzu vines |
| CN106770708A (en) * | 2016-11-18 | 2017-05-31 | 天津中医药大学 | Determine Psoralea corylifolia whether by frying method |
| CN107389838A (en) * | 2017-08-02 | 2017-11-24 | 浙江大学 | The corylifolia L detection methods for commenting method are surveyed based on one more |
| CN107497137A (en) * | 2017-09-18 | 2017-12-22 | 江苏华泰重工装备有限公司 | Plant extract production line |
-
2019
- 2019-09-25 CN CN201910923671.8A patent/CN110638857A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101549010A (en) * | 2009-05-14 | 2009-10-07 | 浙江大学 | A preparing method and application of malaytea scurfpea fruit total glycosides extract |
| CN105833428A (en) * | 2016-05-29 | 2016-08-10 | 洋县新健康医院 | Warming and smoothing medicine bag and making method thereof |
| CN105942539A (en) * | 2016-05-30 | 2016-09-21 | 广西华佳丝绸有限公司 | Method for producing dietary fibers by using mulberry twigs and kudzu vines |
| CN106770708A (en) * | 2016-11-18 | 2017-05-31 | 天津中医药大学 | Determine Psoralea corylifolia whether by frying method |
| CN107389838A (en) * | 2017-08-02 | 2017-11-24 | 浙江大学 | The corylifolia L detection methods for commenting method are surveyed based on one more |
| CN107497137A (en) * | 2017-09-18 | 2017-12-22 | 江苏华泰重工装备有限公司 | Plant extract production line |
Non-Patent Citations (4)
| Title |
|---|
| YANGCHAO CHEN,ET,AL: "Transcriptional regulation of corticotrophin releasing factor gene by furocoumarins isolated from seeds of Psoralea corylifolia", 《LIFE SCIENCES》 * |
| 曹宁校: "体外光化学疗法治疗皮肤T细胞淋巴瘤 ", 《国外医学.皮肤性病学分册》 * |
| 朱耀志: "长波紫外线全身辐照装置", 《中国医疗器械杂志》 * |
| 耿莲，等: "一种由（异）补骨脂苷制备（异）补骨脂素的简单、高效的方法", 《中药材》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111280448A (en) * | 2020-02-14 | 2020-06-16 | 山东博奥克生物科技有限公司 | Sea-buckthorn and cistanche slice and processing production equipment thereof |
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