CN111840466A - Medical health-care product for enhancing immunity and assisting in treating tumors and preparation method thereof - Google Patents

Medical health-care product for enhancing immunity and assisting in treating tumors and preparation method thereof Download PDF

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CN111840466A
CN111840466A CN202010677152.0A CN202010677152A CN111840466A CN 111840466 A CN111840466 A CN 111840466A CN 202010677152 A CN202010677152 A CN 202010677152A CN 111840466 A CN111840466 A CN 111840466A
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parts
extract
extraction
alcohol
enhancing immunity
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杨善彬
张雪华
邓乾华
冯婷婷
李想
夏艳
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Chongqing Normal University
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Chongqing Normal University
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Abstract

The invention belongs to the technical field of medical care and discloses a medical health product for enhancing immunity and assisting in treating tumors and a preparation method thereof, wherein the medical health product for enhancing immunity and assisting in treating tumors is composed of, by mass, 10-20 parts of ginseng, 15-20 parts of achyranthes bidentata, 15-20 parts of houttuynia cordata, 12-24 parts of annona squamosa, 10-15 parts of platycodon grandiflorum, 5-10 parts of lucid ganoderma, 5-8 parts of dendrobe, 5-15 parts of toad, 5-10 parts of jerusalem artichoke, 2-7 parts of medlar and 5-10 parts of gynostemma pentaphylla. The raw materials selected by the invention are traditional medicinal and edible nourishing traditional Chinese medicinal materials, are safe and have no side effect, the medical health care product can effectively inhibit the development of tumors, improve the immunity of human bodies, simultaneously improve the side effects of the tumor patients such as rapid decline of the immunity of the bodies, inappetence, dyspepsia, poor gastrointestinal absorption and the like after chemotherapy and radiotherapy, and improve the life quality of the patients.

Description

Medical health-care product for enhancing immunity and assisting in treating tumors and preparation method thereof
Technical Field
The invention belongs to the technical field of medical care, and particularly relates to a medical health product for enhancing immunity and assisting in treating tumors and a preparation method thereof.
Background
Currently, the world health organization reports show that over 1400 and 820 million people die of cancer patients diagnosed each year. Cancer has become the first leading cause of death in our country, and the number of deaths accounts for one fourth of cancer deaths worldwide and is on the rise year by year.
The clinical treatment generally adopts the methods of radiotherapy and chemotherapy to relieve the pain of tumor patients. However, the immunity of the body of a tumor patient is rapidly reduced after the radiotherapy and the chemotherapy, so that dyspepsia, poor gastrointestinal absorption, extreme weakness and incapability of eating are caused, viruses enter under the condition of deficiency, a series of new diseases appear, and the life health of the patient is influenced. Patients after radiotherapy and chemotherapy also have a series of other adverse reactions, such as dry mouth and bitter taste, sallow complexion, difficult sleep and the like.
Immunity is the body's own defense mechanism, and is the body's ability to recognize and destroy any foreign body (virus, bacteria, etc.) that invades from the outside, to treat aged, damaged, dead, denatured self cells, and to recognize and treat mutant cells and virus-infected cells in the body. Modern immunology considers that immunity is the physiological response of the human body to recognize and eliminate "isohexia". The immune system performs this function in the human body.
The body with low immunity is susceptible to infection, cancer, and the like. The immune system cannot normally exert protective effect due to various reasons, and in this case, the immune system is very easy to infect bacteria, viruses, fungi and the like, so the most direct expression of low immunity is easy to cause diseases. The frequent illness aggravates the consumption of the organism, so the general manifestations of weak constitution, malnutrition, listlessness, fatigue, weakness, appetite reduction, sleep disorder and the like exist, and the patients with illness, injection and medication become the frequent meals of the home. Recovery from each illness can take a long time and often attacks repeatedly. This causes physical and intellectual development failure and also induces serious diseases.
Therefore, how to improve the immunity of the human body and reduce the times of illness of the human body is more important than even improving the medical level.
At present, in daily life, people can enhance the immunity by eating various foods capable of improving the immunity. Such as dairy products, fish, spirulina, etc. However, this approach is slow to take effect and there is no clear medical guidance on the amount and manner of intake.
The effect of the medicine on improving the immunity is more obvious. Western medicine can improve human immunity by providing various vitamins and minerals to human body. However, the root cause problem of low immunity can not be solved in the mode, and excessive intake of minerals and vitamins has certain potential risks to human bodies. Traditional Chinese medicine can fundamentally solve the problem of improving the immunity of human bodies, but the traditional Chinese medicine health care product, namely the fish dragon, on the market at present is mixed, so that the real effect is obvious, and few products are nontoxic.
Through the above analysis, the problems and defects of the prior art are as follows: in the prior art, no medical health care product capable of strengthening immunity and assisting in treating tumors exists.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a medical health-care product for enhancing immunity and assisting in treating tumors and a preparation method thereof.
The invention is realized in such a way that the preparation method of the medical health-care product for enhancing immunity and assisting in treating tumors comprises the following steps:
weighing qualified dried platycodon grandiflorum stem and leaf samples according to a formula, cleaning platycodon grandiflorum 3-5 times by using clear water, and blow-drying by wind flow with the wind speed of 1-1.5 m/s at the temperature of 60-65 ℃.
And step two, putting the dried platycodon grandiflorum stems and leaves into a grinder for grinding, putting the ground platycodon grandiflorum stems and leaves into a grinding disc, grinding into powder, and sieving with a 50-70-mesh sieve.
And step three, putting the crushed platycodon grandiflorum stems and leaves into 10-100% aqueous ethanol solution 7-10 times of the mass of the crushed platycodon grandiflorum stems and leaves, extracting for 2-3 times under pressure, filtering under reduced pressure, and combining the extracting solution to obtain filter residues and alcohol extracting solution.
Step four, placing the combined alcohol extract in a centrifuge for centrifugation, taking supernatant, and reserving base solution; mixing the filter residue with the base solution to obtain a mixed solution; adding compound biological enzyme into the mixed solution for enzymolysis, and filtering to obtain an enzymolysis material.
Step five, adding water to the enzymolysis material obtained in the step four for extraction, filtering to obtain filter residues, and reserving an aqueous extract; extracting the filter residue with low-carbon alcohol for multiple times to obtain multiple alcohol extract; and combining the water extract and the multiple alcohol extracts to obtain the platycodon grandiflorum stem and leaf extract.
Step six, concentrating the extract of the stem and leaf of the platycodon grandiflorum, suspending with water until no precipitate exists, passing through AB-8 type macroporous adsorption resin, eluting with 75% ethanol, collecting an alcohol elution part, and recovering under reduced pressure until no alcohol smell exists; suspending with water to a certain volume, and lyophilizing to obtain the platycodon root stem leaf saponin.
Seventhly, drying and crushing the jerusalem artichoke raw material, mixing the crushed jerusalem artichoke with an organic solvent, leaching for 8-10 hours at the temperature of 40-45 ℃, filtering, and concentrating under reduced pressure to remove ethyl acetate to obtain jerusalem artichoke extract; and (4) removing impurities from the obtained jerusalem artichoke extract.
And step eight, dissolving the jerusalem artichoke extract after impurity removal by using methanol, filtering the filtrate, concentrating the filtrate to be dry, passing through AB-8 type macroporous adsorption resin, eluting by using 75% ethanol, collecting an ethanol elution part, and evaporating to dryness to obtain the jerusalem artichoke extract.
Putting the dried whole gynostemma pentaphylla grass into a grinder, grinding, and sieving with a 45-60-mesh sieve; adding ethanol into the crushed gynostemma pentaphylla, heating and refluxing for extraction, and concentrating and drying the extracting solution under reduced pressure to obtain the gynostemma pentaphylla extract.
And step ten, cutting dried toad skin of the toad to be 1-2 cm long at normal temperature, and crushing the toad skin by using a crusher and sieving the toad skin by using a 180-200-mesh sieve to obtain dried toad skin powder.
Step eleven, mixing the dried toad skin powder with 60% ethanol, and performing ultrasonic extraction at 45-50 ℃; filtering, and freeze drying to obtain the Bufo siccus extract.
And step twelve, mixing the ginseng, the achyranthes bidentata, the houttuynia cordata, the lucid ganoderma, the dendrobium, the Chinese wolfberry and the cherimoya, and soaking for 2-2.5 hours.
And thirteen, putting the soaked ginseng, achyranthes, houttuynia cordata, lucid ganoderma, dendrobium, medlar and annona squamosa into an extraction tank, adding deionized water with the mass of 8-10 times of the ginseng, achyranthes, houttuynia cordata, lucid ganoderma, dendrobium, medlar and annona squamosa, continuously decocting for 3-5 times, combining the decoctions, and concentrating under reduced pressure to obtain a concentrate.
And step fourteen, mixing the concentrate obtained in the step thirteen with the prepared platycodon root stem leaf saponin, the jerusalem artichoke extract, the toad extract and the gynostemma pentaphylla extract, stirring uniformly, and heating in a water bath for 20-30 min.
And step fifteen, performing vacuum drying on the mixture obtained after the water bath heating in the step fourteen to obtain the medical health-care product for enhancing the immunity and assisting in treating the tumor.
Further, in the first step, the pressure extraction of the platycodon grandiflorum stem and leaf powder comprises: the extraction pressure is 10 MPa-100 MPa, and the extraction time is 30-180 min.
Further, in the fourth step, the compound biological enzyme comprises a combination enzyme of cellulase, pectinase and protease.
Further, in the fifth step, the adding water into the enzymolysis material for extraction comprises: the extraction temperature is 80-85 ℃, the extraction time is 2-2.5 h for the first extraction, and 1-1.5 h for the second extraction.
Further, in the fifth step, the multiple low-carbon alcohol extraction of the filter residue comprises: the method comprises the following steps of sequentially extracting for 3-5 times by using 25-32 vol.% of low-carbon alcohol, 45-52 vol.% of low-carbon alcohol and 65-75 vol.% of low-carbon alcohol in an amount which is 3-6 times the mass of the low-carbon alcohol, wherein the extraction time is 0.5-2.5 hours each time, and the alcohol extract obtained after multiple times of extraction is reserved.
Further, the lower alcohol is C5 lower alcohol, and the alcohol extraction adopts counter-current extraction.
Further, in the sixth step, the passing through the AB-8 type macroporous adsorbent resin comprises: the sample loading amount is the extract amount of platycodon grandiflorum stems and leaves: resin amount 0.8: 1, the flow rate of the sample was 1.0 mL/min.
Further, in the seventh step, the organic solvent is ethyl acetate.
Further, in the ninth step, the number of times of heating reflux extraction is 5-7, and the extraction time is 30-35 min each time.
The invention also aims to provide a medical health product for enhancing immunity and assisting in treating tumors, which is prepared by applying the preparation method of the medical health product for enhancing immunity and assisting in treating tumors, and the medical health product for enhancing immunity and assisting in treating tumors is composed of, by mass, 10-20 parts of ginseng, 15-20 parts of achyranthes bidentata, 15-20 parts of houttuynia cordata, 12-24 parts of annona squamosa, 10-15 parts of platycodon grandiflorum, 5-10 parts of lucid ganoderma, 5-8 parts of dendrobium nobile, 5-15 parts of toad, 5-10 parts of Jerusalem artichoke, 2-7 parts of medlar and 5-10 parts of gynostemma pentaphylla.
By combining all the technical schemes, the invention has the advantages and positive effects that: the raw materials selected by the invention are traditional medicinal and edible nourishing traditional Chinese medicinal materials, are safe and have no side effect, the medical health care product can effectively inhibit the development of tumors, improve the immunity of human bodies, simultaneously improve the side effects of the tumor patients such as rapid decline of the immunity of the bodies, inappetence, dyspepsia, poor gastrointestinal absorption and the like after chemotherapy and radiotherapy, and improve the life quality of the patients.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
Fig. 1 is a flow chart of a preparation method of a medical health product for enhancing immunity and assisting in treating tumors provided by the embodiment of the invention.
FIG. 2 is a flow chart of a method for preparing platycodon root stem leaf saponin provided by the embodiment of the invention.
FIG. 3 is a flow chart of the preparation method of Jerusalem artichoke extract according to the embodiment of the invention.
FIG. 4 is a flow chart of a method for preparing the Gynostemma pentaphyllum extract according to the embodiment of the present invention.
FIG. 5 is a flow chart of the process for preparing toad extract according to the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a medical health product for enhancing immunity and assisting in treating tumors and a preparation method thereof, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the preparation method of the medical health product for enhancing immunity and adjuvant therapy of tumor provided by the embodiment of the invention comprises the following steps:
s101, preparing platycodon root stem and leaf saponin, a jerusalem artichoke extract, a toad extract and a gynostemma pentaphylla extract by respectively using platycodon root, jerusalem artichoke, toad and gynostemma pentaphylla.
S102, mixing ginseng, achyranthes, houttuynia, lucid ganoderma, dendrobium, Chinese wolfberry and annona squamosa, and soaking for 2-2.5 hours.
S103, putting the soaked ginseng, the achyranthes bidentata, the houttuynia cordata, the lucid ganoderma, the dendrobium, the wolfberry and the cherimoya into an extraction tank, adding 8-10 times of deionized water by mass, continuously decocting for 3-5 times, combining decoctions, and concentrating under reduced pressure to obtain a concentrate.
And S104, mixing the concentrate obtained in the step S103 with the prepared platycodon root stem and leaf saponin, the jerusalem artichoke extract, the toad extract and the gynostemma pentaphylla extract, uniformly stirring, and heating in a water bath for 20-30 min.
And S105, carrying out vacuum drying on the mixture obtained after the water bath heating of the S104 to obtain the medical health-care product for enhancing the immunity and assisting in treating the tumor.
The medicine health product for enhancing immunity and assisting in treating tumors provided by the embodiment of the invention comprises, by mass, 10-20 parts of ginseng, 15-20 parts of achyranthes bidentata, 15-20 parts of houttuynia cordata, 12-24 parts of annona squamosa, 10-15 parts of platycodon grandiflorum, 5-10 parts of lucid ganoderma, 5-8 parts of dendrobe, 5-15 parts of toad, 5-10 parts of jerusalem artichoke, 2-7 parts of wolfberry and 5-10 parts of gynostemma pentaphylla.
As shown in fig. 2, the method for preparing platycodon grandiflorum stem and leaf saponin provided by the embodiment of the invention comprises the following steps:
s201, weighing qualified dried platycodon grandiflorum stem and leaf samples according to a formula, cleaning platycodon grandiflorum 3-5 times by using clear water, and blow-drying by wind flow with a wind speed of 1-1.5 m/S at an environment of 60-65 ℃.
S202, putting the dried platycodon grandiflorum stems and leaves into a grinder to be ground, putting the ground platycodon grandiflorum stems and leaves into a grinding disc to be ground into powder, and sieving the powder with a 50-70-mesh sieve.
S203, putting the crushed platycodon grandiflorum stems and leaves into 10-100% aqueous ethanol solution 7-10 times of the mass of the crushed platycodon grandiflorum stems and leaves, extracting for 2-3 times under pressure, filtering under reduced pressure, and combining the extracting solution to obtain filter residues and an alcohol extracting solution.
S204, placing the combined alcohol extract in a centrifuge for centrifugation, taking supernatant, and reserving base solution; mixing the filter residue with the base solution to obtain a mixed solution; adding compound biological enzyme into the mixed solution for enzymolysis, and filtering to obtain an enzymolysis material.
S205, adding water into the enzymolysis material obtained in the S204 for extraction, filtering to obtain filter residue, and reserving water extract; extracting the filter residue with low-carbon alcohol for multiple times to obtain multiple alcohol extract; and combining the water extract and the multiple alcohol extracts to obtain the platycodon grandiflorum stem and leaf extract.
S206, concentrating the platycodon grandiflorum stem and leaf extracting solution, suspending with water until no precipitate exists, passing through AB-8 type macroporous adsorption resin, eluting with 75% ethanol, collecting an alcohol eluting part, and recovering under reduced pressure until no alcohol smell exists; suspending with water to a certain volume, and lyophilizing to obtain the platycodon root stem leaf saponin.
In step S201 provided in the embodiment of the present invention, the pressure extraction of the platycodon grandiflorum stem and leaf powder includes: the extraction pressure is 10 MPa-100 MPa, and the extraction time is 30-180 min.
In step S204 provided in the embodiment of the present invention, the compound bio-enzyme includes a combination enzyme of cellulase, pectinase and protease.
In step S205 provided in the embodiment of the present invention, the adding water to extract the enzymolysis material includes: the extraction temperature is 80-85 ℃, the extraction time is 2-2.5 h for the first extraction, and 1-1.5 h for the second extraction.
In step S205 provided in the embodiment of the present invention, the performing multiple low carbon alcohol extractions on the filter residue includes: the method comprises the following steps of sequentially extracting for 3-5 times by using 25-32 vol.% of low-carbon alcohol, 45-52 vol.% of low-carbon alcohol and 65-75 vol.% of low-carbon alcohol in an amount which is 3-6 times the mass of the low-carbon alcohol, wherein the extraction time is 0.5-2.5 hours each time, and the alcohol extract obtained after multiple times of extraction is reserved.
The lower alcohol provided by the embodiment of the invention is C5 lower alcohol, and the alcohol extraction adopts countercurrent extraction.
In step S206 provided in the embodiment of the present invention, the adsorption through the AB-8 type macroporous adsorbent resin includes: the sample loading amount is the extract amount of platycodon grandiflorum stems and leaves: resin amount 0.8: 1, the flow rate of the sample was 1.0 mL/min.
As shown in fig. 3, the method for preparing jerusalem artichoke extract according to the embodiment of the invention comprises:
s301, drying and crushing a jerusalem artichoke raw material, mixing the crushed jerusalem artichoke with an organic solvent, leaching for 8-10 hours at 40-45 ℃, filtering, and concentrating under reduced pressure to remove ethyl acetate to obtain jerusalem artichoke extract; and (4) removing impurities from the obtained jerusalem artichoke extract.
S302, dissolving the jerusalem artichoke extract after impurity removal by using methanol, filtering filtrate, concentrating the filtrate to be dry, passing through AB-8 type macroporous adsorption resin, eluting by using 75% ethanol, collecting an ethanol elution part, and evaporating to dryness to obtain the jerusalem artichoke extract.
In step S301 provided in the embodiment of the present invention, the organic solvent is ethyl acetate.
As shown in fig. 4, the preparation method of the gynostemma pentaphyllum extract provided by the embodiment of the present invention comprises:
s401, placing dried whole gynostemma pentaphylla grass in a grinder, grinding and sieving with a 45-60-mesh sieve.
S402, adding ethanol into the ground gynostemma pentaphylla, heating and refluxing for extraction, and concentrating and drying an extracting solution under reduced pressure to obtain the gynostemma pentaphylla extract.
In step S402 provided in the embodiment of the present invention, the number of times of heating reflux extraction is 5 to 7, and the extraction time is 30 to 35min each time.
As shown in fig. 5, the preparation method of toad extract provided by the embodiment of the invention comprises:
s501, cutting dried toad skin of a toad to be 1-2 cm long at normal temperature, and crushing the toad skin by using a crusher and sieving the toad skin with a 180-200-mesh sieve to obtain dried toad skin powder.
S502, mixing the dried toad skin powder with 60% ethanol, and performing ultrasonic extraction at 45-50 ℃; filtering, and freeze drying to obtain the Bufo siccus extract.
The technical solution of the present invention is further described with reference to the following specific embodiments.
Example 1:
the preparation method of the medical health-care product for enhancing immunity and assisting in treating tumors provided by the embodiment 1 of the invention comprises the following steps:
step one, preparing platycodon stem and leaf saponin, a jerusalem artichoke extract, a toad extract and a gynostemma pentaphylla extract by respectively using 5 parts of platycodon grandiflorum, 5 parts of jerusalem artichoke, 5 parts of toad and 5 parts of gynostemma pentaphylla;
step two, mixing 10 parts of ginseng, 15 parts of achyranthes root, 15 parts of houttuynia cordata, 5 parts of lucid ganoderma, 5 parts of dendrobium, 2 parts of medlar and 12 parts of sweetsop, and soaking for 2 hours;
step three, putting the soaked ginseng, achyranthes root, houttuynia cordata, lucid ganoderma, dendrobium, medlar and sweetsop into an extraction tank, adding 8 times of deionized water, continuously decocting for 3 times, combining the decoctions, and concentrating under reduced pressure to obtain a concentrate;
step four, mixing the concentrate obtained in the step three with the platycodon root stem and leaf saponin, the jerusalem artichoke extract, the toad extract and the gynostemma pentaphylla extract obtained in the step one, stirring uniformly, and heating in a water bath for 20 min;
and step five, drying the mixture heated in the step four in vacuum to obtain the medical health-care product for enhancing immunity and assisting in treating tumors.
Example 2:
the preparation method of the medical health-care product for enhancing immunity and assisting in treating tumors, which is provided by the embodiment 2 of the invention, comprises the following steps:
step one, preparing platycodon stem and leaf saponin, a jerusalem artichoke extract, a toad extract and a gynostemma pentaphylla extract by respectively using 7 parts of platycodon grandiflorum, 5 parts of jerusalem artichoke, 7 parts of toad and 6 parts of gynostemma pentaphylla;
step two, taking 15 parts of ginseng, 16 parts of achyranthes root, 18 parts of houttuynia cordata, 8 parts of lucid ganoderma, 7 parts of dendrobium, 5 parts of medlar and 20 parts of sweetsop, mixing and soaking for 2.2 hours;
step three, putting the soaked ginseng, achyranthes root, houttuynia cordata, lucid ganoderma, dendrobium, medlar and sweetsop into an extraction tank, adding 9 times of deionized water, continuously decocting for 4 times, combining the decoctions, and concentrating under reduced pressure to obtain a concentrate;
step four, mixing the concentrate obtained in the step three with the platycodon root stem and leaf saponin, the jerusalem artichoke extract, the toad extract and the gynostemma pentaphylla extract obtained in the step one, stirring uniformly, and heating in a water bath for 25 min;
and step five, drying the mixture heated in the step four in vacuum to obtain the medical health-care product for enhancing immunity and assisting in treating tumors.
Example 3:
the preparation method of the medical health-care product for enhancing immunity and assisting in treating tumors provided by the embodiment 3 of the invention comprises the following steps:
Step one, preparing platycodon stem and leaf saponin, a jerusalem artichoke extract, a toad extract and a gynostemma pentaphylla extract by respectively using 10 parts of platycodon grandiflorum, 10 parts of jerusalem artichoke, 10 parts of toad and 10 parts of gynostemma pentaphylla;
step two, mixing 20 parts of ginseng, 20 parts of achyranthes root, 20 parts of houttuynia cordata, 10 parts of lucid ganoderma, 8 parts of dendrobium, 7 parts of medlar and 24 parts of sweetsop, and soaking for 2.5 hours;
step three, putting the soaked ginseng, achyranthes root, houttuynia cordata, lucid ganoderma, dendrobium, medlar and sweetsop into an extraction tank, adding 10 times of deionized water, continuously decocting for 5 times, combining the decoctions, and concentrating under reduced pressure to obtain a concentrate;
step four, mixing the concentrate obtained in the step three with the platycodon root stem and leaf saponin, the jerusalem artichoke extract, the toad extract and the gynostemma pentaphylla extract obtained in the step one, stirring uniformly, and heating in a water bath for 30 min;
and step five, drying the mixture heated in the step four in vacuum to obtain the medical health-care product for enhancing immunity and assisting in treating tumors.
The technical effects of the present invention will be further explained below in conjunction with pharmacological analysis.
Ginseng: sweet and slightly bitter in taste, warm and mild in nature. It enters spleen, lung and heart meridians. Tonify qi, secure collapse, promote fluid production, induce tranquilization, and promote intelligence. Tonify primordial qi, recover pulse to stop collapse, tonify spleen and lung, promote fluid production, and induce tranquilization. The succulent root of ginseng is a well-known tonic, is suitable for regulating blood pressure, recovering heart function, neurasthenia and asthenia, and also has expectorant, stomach invigorating, diuretic and exciting effects.
Achyranthes root: bitter, sweet, sour and neutral. It enters liver and kidney meridians. The efficacy is as follows: dispel blood stasis and dredge channels, tonify liver and kidney, strengthen tendons and bones, induce diuresis and treat stranguria, and draw blood downward. The main treatment is as follows: can be used for treating amenorrhea, dysmenorrhea, soreness of waist and knees, myasthenia of bones and muscles, stranguria, edema, headache, vertigo, toothache, skin ulcer, hematemesis, and epistaxis.
Houttuynia cordata: yu xing Cao is pungent in flavor and cold in nature and enters lung meridian. Has effects in clearing away heat and toxic materials, relieving swelling, treating sore, promoting urination, removing dampness, clearing away heat, relieving dysentery, invigorating stomach, and promoting digestion, and can be used for treating lung abscess, pyocutaneous disease, hematochezia, and heat accumulation in spleen and stomach due to excess heat, heat toxin, dampness, and disease heat.
Ganoderma lucidum: warm in nature and bland in taste. The main functional indications are as follows: has tonic effect. It has brain strengthening, antiinflammatory, diuretic, and kidney invigorating effects.
Dendrobe: sweet in flavor and slightly cold in nature. Has the effects of benefiting stomach, promoting fluid production, nourishing yin and clearing heat. It is commonly indicated for heat disease with body fluid deficiency, dry mouth, polydipsia, stomach yin deficiency, poor appetite, retching, persistent asthenic fever after disease, hyperactivity of fire due to yin deficiency, bone steaming, fatigue heat, dim eyesight, flaccidity of tendons and bones.
Balloon flower: bitter and pungent in flavor and mild in nature. Has effects of dispersing lung qi, relieving sore throat, eliminating phlegm, and expelling pus. Can be used for treating cough with excessive phlegm, chest distress, pharyngalgia, hoarseness, lung carbuncle, and purulent vomiting.
Toad: pungent, cool and toxic. Breaking the knot of disease, promoting the flow of water-dampness, resolving toxin, killing parasites and relieving pain. It is used to treat furuncle, carbuncle of the yin-yang, scrofula, malignant boil, abdominal mass, tympanites, edema, infantile malnutrition, and chronic tracheitis.
Annona squamosa: sweet in flavor and cold in nature. Has the functions of invigorating spleen and stomach, clearing away heat and toxic material and killing pests. It is commonly used for sore and swelling pain and intestinal parasitosis.
Balloon flower: bitter and pungent in flavor and mild in nature. Has effects of dispersing lung qi, relieving sore throat, eliminating phlegm, and expelling pus. Can be used for treating cough with excessive phlegm, chest distress, pharyngalgia, hoarseness, lung carbuncle, and purulent vomiting.
Jerusalem artichoke: sweet in taste; slightly bitter; is cool in nature. The main functional indications are as follows: clear heat and cool blood, and heal bone. It can be used for treating pyrexia, intestinal heat, hemorrhage, traumatic injury, and bone injury.
Gynostemma pentaphylla: cold in nature and bitter in taste. The main functional indications are as follows: diminish inflammation, remove toxicity, relieve cough and eliminate phlegm. It is used as tonic.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.

Claims (10)

1. The preparation method of the medical health-care product for enhancing immunity and assisting in treating tumors is characterized by comprising the following steps of:
Weighing qualified dried platycodon grandiflorum stem and leaf samples according to a formula, cleaning platycodon grandiflorum 3-5 times by using clear water, and drying the platycodon grandiflorum in an environment at the temperature of 60-65 ℃ by using wind flow with the wind speed of 1-1.5 m/s;
putting the dried platycodon grandiflorum stems and leaves into a grinder for grinding, putting the ground platycodon grandiflorum stems and leaves into a grinding disc, grinding into powder, and sieving with a 50-70-mesh sieve;
putting the crushed platycodon grandiflorum stems and leaves into 10-100% aqueous ethanol solution 7-10 times of the mass of the crushed platycodon grandiflorum stems and leaves, extracting for 2-3 times under pressure, filtering under reduced pressure, and combining extracting solutions to obtain filter residues and alcohol extracting solution;
step four, placing the combined alcohol extract in a centrifuge for centrifugation, taking supernatant, and reserving base solution; mixing the filter residue with the base solution to obtain a mixed solution; adding compound biological enzyme into the mixed solution for enzymolysis, and filtering to obtain an enzymolysis material;
step five, adding water to the enzymolysis material obtained in the step four for extraction, filtering to obtain filter residues, and reserving an aqueous extract; extracting the filter residue with low-carbon alcohol for multiple times to obtain multiple alcohol extract; combining the water extract and the multiple alcohol extracts to obtain a platycodon grandiflorum stem and leaf extract;
step six, concentrating the extract of the stem and leaf of the platycodon grandiflorum, suspending with water until no precipitate exists, passing through AB-8 type macroporous adsorption resin, eluting with 75% ethanol, collecting an alcohol elution part, and recovering under reduced pressure until no alcohol smell exists; suspending with water to a certain volume, and lyophilizing to obtain the saponin of stem and leaf of radix Platycodi;
Seventhly, drying and crushing the jerusalem artichoke raw material, mixing the crushed jerusalem artichoke with an organic solvent, leaching for 8-10 hours at the temperature of 40-45 ℃, filtering, and concentrating under reduced pressure to remove ethyl acetate to obtain jerusalem artichoke extract; removing impurities from the obtained Jerusalem artichoke extract;
step eight, dissolving the jerusalem artichoke extract after impurity removal by using methanol, filtering filtrate, concentrating the filtrate to be dry, eluting the filtrate by using 75% ethanol through AB-8 type macroporous adsorption resin, collecting an alcohol elution part, and evaporating the alcohol elution part to dryness to obtain the jerusalem artichoke extract;
putting the dried whole gynostemma pentaphylla grass into a grinder, grinding, and sieving with a 45-60-mesh sieve; adding ethanol into the crushed gynostemma pentaphylla, heating, refluxing and extracting, and concentrating and drying an extracting solution under reduced pressure to obtain the gynostemma pentaphylla extract;
step ten, cutting dried toad skin of the toads to be 1-2 cm long at normal temperature, and crushing the toad skin by using a crusher and sieving the toad skin by using a 180-200-mesh sieve to obtain dried toad skin powder;
step eleven, mixing the dried toad skin powder with 60% ethanol, and performing ultrasonic extraction at 45-50 ℃; filtering, and freeze drying to obtain Bufo siccus extract;
step twelve, mixing ginseng, achyranthes root, houttuynia cordata, lucid ganoderma, dendrobium, Chinese wolfberry and annona squamosa, and soaking for 2-2.5 hours;
Step thirteen, putting the soaked ginseng, achyranthes, houttuynia, ganoderma, dendrobium, medlar and annona squamosa into an extraction tank, adding deionized water with the mass of 8-10 times of that of the ginseng, achyranthes, houttuynia, ganoderma, dendrobium, medlar and annona squamosa, continuously decocting for 3-5 times, combining the decoctions, and concentrating under reduced pressure to obtain a concentrate;
step fourteen, mixing the concentrate obtained in the step thirteen with prepared platycodon root stem and leaf saponin, jerusalem artichoke extract, toad extract and gynostemma pentaphylla extract, stirring uniformly, and heating in a water bath for 20-30 min;
and step fifteen, performing vacuum drying on the mixture obtained after the water bath heating in the step fourteen to obtain the medical health-care product for enhancing the immunity and assisting in treating the tumor.
2. The method for preparing a medical health product for enhancing immunity and assisting in treating tumors as claimed in claim 1, wherein in the first step, the pressure extraction of the platycodon grandiflorum stem and leaf powder comprises the following steps: the extraction pressure is 10 MPa-100 MPa, and the extraction time is 30-180 min.
3. The method for preparing a health product for enhancing immunity and adjuvant therapy of tumor as claimed in claim 1, wherein in step four, the compound bio-enzyme comprises a combination of cellulase, pectinase and protease.
4. The preparation method of the medical health product for enhancing immunity and assisting in treating tumors as claimed in claim 1, wherein in the fifth step, the water-adding extraction of the enzymolysis material comprises the following steps: the extraction temperature is 80-85 ℃, the extraction time is 2-2.5 h for the first extraction, and 1-1.5 h for the second extraction.
5. The method for preparing a health product for enhancing immunity and adjuvant therapy of tumor as claimed in claim 1, wherein in the step five, the multiple low carbon alcohol extraction of the residue comprises: the method comprises the following steps of sequentially extracting for 3-5 times by using 25-32 vol.% of low-carbon alcohol, 45-52 vol.% of low-carbon alcohol and 65-75 vol.% of low-carbon alcohol in an amount which is 3-6 times the mass of the low-carbon alcohol, wherein the extraction time is 0.5-2.5 hours each time, and the alcohol extract obtained after multiple times of extraction is reserved.
6. The method for preparing a health product for enhancing immunity and adjuvant therapy of tumor as claimed in claim 5, wherein the lower alcohol is C5 lower alcohol, and the alcohol extraction is performed by countercurrent extraction.
7. The method for preparing a health product for enhancing immunity and adjuvant therapy of tumor as claimed in claim 1, wherein in step six, passing through AB-8 type macroporous adsorbent resin comprises: the sample loading amount is the extract amount of platycodon grandiflorum stems and leaves: resin amount 0.8: 1, the flow rate of the sample was 1.0 mL/min.
8. The method for preparing a health product for enhancing immunity and adjuvant therapy of tumor as claimed in claim 1, wherein in step seven, the organic solvent is ethyl acetate.
9. The method for preparing a health product for enhancing immunity and adjuvant therapy of tumor as claimed in claim 1, wherein in the ninth step, the number of times of heating reflux extraction is 5-7 times, and the extraction time is 30-35 min each time.
10. The medicine health product for enhancing immunity and assisting in treating tumors, which is prepared by the preparation method of the medicine health product for enhancing immunity and assisting in treating tumors according to any one of claims 1 to 9, is characterized by comprising, by mass, 10 to 20 parts of ginseng, 15 to 20 parts of achyranthes bidentata, 15 to 20 parts of houttuynia cordata, 12 to 24 parts of annona squamosa, 10 to 15 parts of platycodon grandiflorum, 5 to 10 parts of lucid ganoderma, 5 to 8 parts of dendrobe, 5 to 15 parts of toad, 5 to 10 parts of jerusalem artichoke, 2 to 7 parts of medlar and 5 to 10 parts of gynostemma pentaphylla.
CN202010677152.0A 2020-07-14 2020-07-14 Medical health-care product for enhancing immunity and assisting in treating tumors and preparation method thereof Pending CN111840466A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113244372A (en) * 2021-04-22 2021-08-13 建昌帮药业有限公司 Composition for enhancing immunity and preparation process and application thereof
CN113785977A (en) * 2021-03-11 2021-12-14 重庆师范大学 Health food for clearing heat from throat, relieving cough and moistening lung and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
牛林敬等: "《养肾补虚看这本就够了》", 31 January 2017, 河北科学技术出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113785977A (en) * 2021-03-11 2021-12-14 重庆师范大学 Health food for clearing heat from throat, relieving cough and moistening lung and preparation method thereof
CN113244372A (en) * 2021-04-22 2021-08-13 建昌帮药业有限公司 Composition for enhancing immunity and preparation process and application thereof

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