CN101549010A - A preparing method and application of malaytea scurfpea fruit total glycosides extract - Google Patents
A preparing method and application of malaytea scurfpea fruit total glycosides extract Download PDFInfo
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- CN101549010A CN101549010A CNA2009100987671A CN200910098767A CN101549010A CN 101549010 A CN101549010 A CN 101549010A CN A2009100987671 A CNA2009100987671 A CN A2009100987671A CN 200910098767 A CN200910098767 A CN 200910098767A CN 101549010 A CN101549010 A CN 101549010A
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Abstract
The present invention provides a method of preparing malaytea scurfpea fruit total glycoside extract, including: using water to extracting the malaytea scurfpea fruit, concentrating the extract and then performing alcohol deposition, using small polar organic reagent to extract the supernatant, using macroporous resin for purifying the aqueous layer after extraction to obtain the total malaytea scurfpea fruit glycoside extract, wherein, the content of the total malaytea scurfpea fruit glycosides is more than 80%, which contains 40-50% of malaytea scurfpea fruit glycosides, 40-50% of isopsoralen glycosides by HPLC content measuring. The malaytea scurfpea fruit total glycoside extract provided by the present invention is authenticated by pharmacological experiments that it can fight against cyclophosphamide-induced leukopenia, has a significant role in immune enhancement, and can be applied in the medicament for increasing leucocyte. The inventive method obtains malaytea scurfpea fruit total glycosides from the Chinese medicine malaytea scurfpea fruit, which is simple, convenient, economic, stable, easy to operate, and is suitable for industrial mass production.
Description
Technical field
The invention belongs to field of medicaments, through extracting, making with extra care, obtain the method for total Fructus Psoraleae glucoside extract in the bone fat that particularly relates to therefrom build up one's health by taking tonic, and the medicinal usage of this extract.
Background technology
At present, in the world, Chinese herbal medicine all has certain market, along with people increasing and the aging of population to the health requirements level of understanding, sub-health stateization, people thirst for back to nature more, utilize the high Drug therapy of pure natural degree, prevent some chemical synthetic drugs cann't be solved problem, so the application of natural plant exceeds the background of its original traditional national culture.From natural drug, seek the little and inexpensive medicine of side effect and become the target that countries in the world pharmaceutical manufacturer is chased.The European Community has carried out unified legislation to medical herbs, state medical herbs status such as Canada and Australia have legalized, U.S. government has also drafted the plant amedica management method, the compound recipe mix preparation that begins to accept natural drug is as curative, and these provide good international environment for Chinese medicine enters international medical market as curative.On the other hand, along with the quickening of global economic integration progress, particularly China becomes a full member of WTO, and Chinese Medicine market incorporates the breadth and depth of international medical big market and will further aggravate.Face the enormous impact of Asian countries's traditional medicine product such as the keen competition of powerful transnational medical group and Japan, Korea S, India, Thailand and European countries' plant amedica such as Germany, France, numerous products that China's Chinese medicine produces are owing to still can not meet the standard of international medical market and requirement and being kept outside of the door.
The Chinese medicine Fructus Psoraleae is legumes psoraleae (Psoralea corylifolia L) fruit, and is warm in nature, acrid in the mouth, the effect of tool reinforcing the kidney and supporting YANG.Cure mainly the cold diarrhea of suffering from a deficiency of the kidney, frequent micturition, sexual impotence, chills and pain of the waist and kness, deficiency and coldness is breathed with cough, the external curing vitiligo.Modern study shows that Fructus Psoraleae has blood vessel dilating, increases myocardial contraction, and is antibiotic, the effect of estrogen sample, physiologically actives such as treatment vitiligo.Fructus Psoraleae contains the various active composition, and the glycoside that is representative with Fructus Psoraleae glycosides and different Fructus Psoraleae glycosides is one of its important active component, but present document and patent still do not have the report that obtains the Fructus Psoraleae glycoside.
Summary of the invention
The object of the present invention is to provide a kind of low cost, easy to operate, good stability, be easy to the preparation method of the malaytea scurfpea fruit total glycosides extract of industrialization, and representative composition is wherein carried out assay.
Preparation method of the present invention is achieved through the following technical solutions:
(1) the Fructus Psoraleae water is cooked solvent extraction;
(2) extracting solution concentrates the back precipitate with ethanol;
(3) the precipitate with ethanol supernatant extracts with little polarity organic reagent;
(4) extraction back water layer purification by macroporous resin gets total Fructus Psoraleae glucoside extract, and wherein total Fructus Psoraleae glycosides content is more than 80%;
Wherein: in the step (1), solvent load 8-15 doubly measures, heating extraction 1-3 time, each 0.5-2 hour.The optimum solvent consumption is 10 times of amounts, heating extraction 2 times, each 2 hours.
In the step (2), concentration of alcohol is 70-95%, and best concentration of alcohol is 80%.
In the step (3), little polarity organic reagent is selected petroleum ether for use, chloroform or ethyl acetate.
In the step (4), extraction back water layer is by the macroporous adsorption resin chromatography post, and first water washes, and eluent is collected in the flushing of reuse ethanol water, and eluent is evaporated to does not have alcohol, and lyophilization promptly gets total Fructus Psoraleae glycosides.Wherein the ethanol water determining alcohol is 30-100%.Carry out assay by HPLC, Fructus Psoraleae glycosides (Psoralenoside) content is 40-50% in the malaytea scurfpea fruit total glycosides, and different Fructus Psoraleae glycosides (Isopsoralenoside) content is 40-50%.
Another object of the present invention provides the application of described malaytea scurfpea fruit total glycosides extract in the medicine of preparation leukocyte increasing.
The malaytea scurfpea fruit total glycosides extract that the inventive method preparation is stated, the dressing or the excipient that allow with preparation are prepared into said dosage form on any pharmaceutics, also can cooperate other drug or component to make pharmaceutical preparation together.
Pharmaceutical dosage form can be tablet, capsule, granule, oral liquid, slow releasing preparation, controlled release preparation, gel, ointment, ointment, cream, suppository, injection, injectable powder, patch, drop pill, suspensoid etc.
The method of the invention adopts simple process flow therefrom to build up one's health by taking tonic and obtains malaytea scurfpea fruit total glycosides in the bone fat, and wherein Fructus Psoraleae glycosides and different Fructus Psoraleae glycosides are carried out assay, and method is easy, economical, is beneficial to large-scale industrialization production.Prove through pharmacological evaluation, this malaytea scurfpea fruit total glycosides extract with and the Fructus Psoraleae glycosides that contains and the different Fructus Psoraleae glycosides leucocytes reduction that all can resist caused by cyclophosphamide, have significant immunological enhancement, can in the medicine of preparation leukocyte increasing, use.
Description of drawings
Fig. 1 malaytea scurfpea fruit total glycosides chromatogram.
The specific embodiment
The present invention is further described in conjunction with the embodiments.The embodiment of the invention only is used for explanation and is not limitation of the present invention.
The HPLC of embodiment one Fructus Psoraleae glycosides and different Fructus Psoraleae glycosides analyzes and content assaying method
Chromatographic condition chromatographic column Agilent Zorbax SB-C18 post (4.6mm * 250mm, 5 μ m); Adopt gradient elution, mobile phase A is 0.1% glacial acetic acid aqueous solution mutually, and Mobile phase B is mutually for containing the acetonitrile solution of 0.1% glacial acetic acid; The gradient elution program is as follows: in the time of 0 minute, mobile phase is 95% A, 5% B; In the time of 20 minutes, mobile phase is 70% A, 30% B; In the time of 25 minutes, mobile phase is 50% A, 50% B; In the time of 30 minutes, mobile phase is 5% A, 95% B.Solution flow rate 1.0mLmin
-1Detect wavelength 246nm; 30 ℃ of column temperatures can be measured the content of Fructus Psoraleae glycosides and different Fructus Psoraleae glycosides.Referring to Fig. 1,1 is different Fructus Psoraleae glycosides among the figure, and 2 is the Fructus Psoraleae glycosides.
Embodiment two
Get Fructus Psoraleae 1kg, 50 ℃ of dryings 6 hours add 8 times of water gaging slight boiling condition heating and refluxing extraction 2 hours, extracting liquid filtering, concentrating under reduced pressure, precipitate with ethanol, regulate determining alcohol to 80%, get supernatant, add ethyl acetate extraction after being concentrated into no alcohol, separate water layer, last macroporous resin, the water flushing discards, and continues with 30% ethanol water eluting.Collect eluent, being evaporated to does not have alcohol, and lyophilization promptly gets malaytea scurfpea fruit total glycosides.Press embodiment one method and measure, Fructus Psoraleae glycosides content is 38% in total Fructus Psoraleae glycosides, and different Fructus Psoraleae glycosides content is 42%.
Embodiment three
Get Fructus Psoraleae 1kg, 50 ℃ of dryings 6 hours add 8 times of water gaging slight boiling condition heating and refluxing extraction 2 hours, extracting liquid filtering, concentrating under reduced pressure, precipitate with ethanol, regulate determining alcohol to 70%, get supernatant, add ethyl acetate extraction after being concentrated into no alcohol, separate water layer, last macroporous resin, the water flushing discards, and continues with 50% ethanol water eluting.Collect eluent, being evaporated to does not have alcohol, and lyophilization promptly gets malaytea scurfpea fruit total glycosides.Press embodiment one method and measure, Fructus Psoraleae glycosides content is 40% in total Fructus Psoraleae glycosides, and different Fructus Psoraleae glycosides content is 42%.
Embodiment four
Get Fructus Psoraleae 1kg, 50 ℃ of dryings 6 hours add 12 times of water gaging slight boiling condition heating and refluxing extraction 2 hours, extracting liquid filtering, concentrating under reduced pressure, precipitate with ethanol, regulate determining alcohol to 70%, get supernatant, add ethyl acetate extraction after being concentrated into no alcohol, separate water layer, last macroporous resin, the water flushing discards, and continues with 70% ethanol water eluting.Collect eluent, being evaporated to does not have alcohol, and lyophilization promptly gets malaytea scurfpea fruit total glycosides.Press embodiment one method and measure, Fructus Psoraleae glycosides content is 41% in total Fructus Psoraleae glycosides, and different Fructus Psoraleae glycosides content is 43%.
Embodiment five
Get Fructus Psoraleae 1kg, 50 ℃ of dryings 6 hours add 10 times of water gaging slight boiling condition heating and refluxing extraction 2 times, each 1 hour, extracting liquid filtering, concentrating under reduced pressure, precipitate with ethanol is regulated determining alcohol to 80%, gets supernatant, add chloroform extraction 2 times after being concentrated into no alcohol, separate water layer, last macroporous resin, water flushing, discard, continue with 80% ethanol water eluting.Collect eluent, being evaporated to does not have alcohol, and lyophilization promptly gets malaytea scurfpea fruit total glycosides.Press embodiment one method and measure, Fructus Psoraleae glycosides content is 43% in total Fructus Psoraleae glycosides, and different Fructus Psoraleae glycosides content is 41%.
Embodiment six
Get Fructus Psoraleae 1kg, 50 ℃ of dryings 6 hours add 10 times of water gaging slight boiling condition heating and refluxing extraction 2 times, each 2 hours, extracting liquid filtering, concentrating under reduced pressure, precipitate with ethanol is regulated determining alcohol to 70%, gets supernatant, add ethyl acetate extraction 3 times after being concentrated into no alcohol, separate water layer, last macroporous resin, water wash to sugar-free (molish reaction), discard, continue with 70% ethanol water eluting.Collect eluent, being evaporated to does not have alcohol, and lyophilization promptly gets malaytea scurfpea fruit total glycosides.Press embodiment one method and measure, Fructus Psoraleae glycosides content is 46% in total Fructus Psoraleae glycosides, and different Fructus Psoraleae glycosides content is 45%.
The preparation of embodiment seven drop pill
Get malaytea scurfpea fruit total glycosides 0.5g and 10.5g Polyethylene Glycol-6000 mix homogeneously, heating and melting moves in the drop pill drip irrigation behind the change material, and in ℃ liquid paraffin of medicine liquid droplet to 6～8, oil removing makes 300 of drop pill.
The preparation of embodiment eight lyophilized injectable powders
Get Lignum Dalbergiae Odoriferae oil 1.5g, join in the saturated hydroxypropyl of 13ml, stirring and dissolving filters, and the filtrate cold drying gets the clathrate powder of Lignum Dalbergiae Odoriferae oil and hydroxypropyl.Except that above-mentioned Lignum Dalbergiae Odoriferae oil closes the clathrate powder of hydroxypropyl, get malaytea scurfpea fruit total glycosides 0.5g, mannitol 5.5g, calcium disodium edetate 0.9g and distilled water 2ml again, behind the said components mixing, lyophilization, 350 of packing, promptly.
The preparation of embodiment nine tablets
Get malaytea scurfpea fruit total glycosides 100 gram and microcrystalline Cellulose mix homogeneously, add 3% polyvidone alcoholic solution system soft material, cross 18 mesh sieve system granules, 60 ℃ of dryings 1 hour, granulate, the adding Pulvis Talci is an amount of, mixing, tabletting, promptly.
Embodiment ten capsular preparations
Get malaytea scurfpea fruit total glycosides and Oleum Arachidis hypogaeae semen in 2: 18 the ratio water dissolving type rustless steel stirring filling, add an amount of gelatin and glycerol simultaneously and mix, emit reuse colloid mill defibrination, the mixed liquor that grinds stirs, and makes medicinal liquid; Under 40-50 ℃ of temperature, stir glycerol and distilled water miscible, again with the glycerin liquid temperature to 80-90 ℃, get gelatin and add wherein to mix and stir, become glue until off-bottom, glue was left standstill 4 hours, make offset plate with laminator and use for the pill operation; Above-mentioned medicinal liquid of making and offset plate are sent into the pellet press pill, did cylinder typing in 4 hours at 22-25 ℃ of temperature canyon then, again dry 16-20 hour of 25-30 ℃ of temperature canyon, the qualified soft gelatin capsule of pick, clean with 95% ethanol, dry 4 hours of 25-30 ℃ of canyon, promptly.
The preparation of embodiment 11 injection
Get malaytea scurfpea fruit total glycosides 100 grams with 40 ℃ of water for injection dissolvings, add an amount of pharmaceutical carrier isotonic agent, the pH value of regulator solution is 7.0-8.0, adds to the full amount of water for injection, remove thermal source with the ultrafilter ultrafiltration, behind the mensuration pH value, use membrane filtration, after the packing, autoclaving, check, packing, promptly.
The influence that embodiment 12 malaytea scurfpea fruit total glycosides reduce caused by cyclophosphamide normal mouse numeration of leukocyte
1. experiment purpose: investigate the influence of malaytea scurfpea fruit total glycosides to the caused by cyclophosphamide leucocytes reduction.
2. experiment material
2.1 medicine and reagent: Colla Corii Asini, Dong-E donkey-hide Gelatin Co., Ltd., Shandong Prov., lot number: 0311101; Cyclophosphamide for injection (CTX), Hengrui Medicine Co., Ltd., Jiangsu Prov., lot number: 7063021; The leukocyte diluent: glacial acetic acid 2ml, 1% Gentian Violet 1ml, distilled water adds to 100ml.
2.2 experiment equipment: cell counting count board, microscope, 1000 μ l, 100 μ l micropipettors.
2.3 laboratory animal: the ICR mice, male and female double, body weight 18-22g is provided by the Zhejiang Academy of Medical Sciences animal center.
3. method and step
3.1 get healthy 18-22g ICR mice, under this laboratory condition, raise after 2 days random packet:
Normal control group: isometric(al) normal saline
Model group: isometric(al) normal saline
Colla Corii Asini group: 1.5gKg
-1
Experimental group: low, high dose group (crude drug amount 2gKg
-1, 4gKg
-1);
Every group 10.Each organized the mice successive administration 8 days.
3.2 administration the 3rd day, every mouse peritoneal injection cyclophosphamide 100mg/kg (0.2ml/10g).
3.3 behind the 8th day (injection cyclophosphamide after the 5th day) administration 2h, the eye socket vein is got blood, draws 20 μ l blood, changes 380 μ l leukocyte diluents immediately over to, shakes gently 1～2 minute, fully counts behind the mixing.
4. experimental result
Experimental result shows, behind the injection cyclophosphamide the 5th day, the model group numeration of leukocyte was (3.33 ± 0.98) * 10
6/ ml is than normal group (7.24 ± 1.62) * 10
6/ ml obviously reduces, difference significance (P＜0.01).Low dose group and high dose group numeration of leukocyte are (4.05 ± 0.80) * 10
6/ ml, (4.29 ± 1.37) * 10
6/ ml obviously raises than model group, difference significance (P＜0.05, P＜0.05).Positive controls (Colla Corii Asini group) numeration of leukocyte is (4.05 ± 0.83) * 10
6/ ml obviously raises than model group, difference significance (P＜0.05).
The influence that table 1 malaytea scurfpea fruit total glycosides reduces caused by cyclophosphamide normal mouse numeration of leukocyte (x ± s, n=10)
Annotate: compare with normal group
*P＜0.05,
*P＜0.01;
Compare with model group
△P＜0.05,
△ △P＜0.01.
5. conclusion
The leucocytes reduction that malaytea scurfpea fruit total glycosides is low, high dose all can resist caused by cyclophosphamide.
The influence that embodiment 13 Fructus Psoraleae glycosides reduce caused by cyclophosphamide normal mouse numeration of leukocyte
1. experiment purpose: investigate of the influence of Fructus Psoraleae glycosides to the caused by cyclophosphamide leucocytes reduction.
2. experiment material
2.1 medicine and reagent
Colla Corii Asini, Dong-E donkey-hide Gelatin Co., Ltd., Shandong Prov., lot number: 0311101;
Cyclophosphamide for injection (CTX), Hengrui Medicine Co., Ltd., Jiangsu Prov., lot number: 07063021;
The leukocyte diluent: glacial acetic acid 2ml, 1% Gentian Violet 1ml, distilled water adds to 100ml.
2.2 experiment equipment: cell counting count board, microscope, 1000 μ l, 100 μ l micropipettors.
2.3 laboratory animal
The ICR mice, male and female double, body weight 18-22g is provided by the Zhejiang Academy of Medical Sciences animal center.
3. method and step
3.1 get healthy 18-22g ICR mice, under this laboratory condition, raise after 2 days random packet:
Normal control group: isometric(al) normal saline
Model group: isometric(al) normal saline
Colla Corii Asini group: 1.5gKg
-1
Experimental group: low, high dose group (0.1gKg
-1, 0.3gKg
-1);
Every group 10.Each organized the mice successive administration 8 days.
3.2 administration the 3rd day, every mouse peritoneal injection cyclophosphamide 100mg/kg (0.2ml/10g).
3.3 behind the 8th day (injection cyclophosphamide after the 5th day) administration 2h, the eye socket vein is got blood, draws 20 μ l blood, changes 380 μ l leukocyte diluents immediately over to, shakes gently 1～2 minute, fully counts behind the mixing.
4. experimental result
Experimental result shows, behind the injection cyclophosphamide the 5th day, the model group numeration of leukocyte was (3.33 ± 0.98) * 10
6/ ml is than normal group (7.24 ± 1.62) * 10
6/ ml obviously reduces, difference significance (P＜0.01).Low dose group and high dose group numeration of leukocyte are (3.82 ± 0.66) * 10
6/ ml, (3.99 ± 1.12) * 10
6/ ml obviously raises than model group, difference significance (P＜0.05, P＜0.05).Positive controls (Colla Corii Asini group) numeration of leukocyte is (4.05 ± 0.83) * 10
6/ ml obviously raises than model group, difference significance (P＜0.05).
The influence that table 2 Fructus Psoraleae glycosides reduces caused by cyclophosphamide normal mouse numeration of leukocyte (x ± s, n=10)
Annotate: compare with normal group
*P＜0.05,
*P＜0.01;
Compare with model group
△P＜0.05,
△ △P＜0.01.
5. conclusion
The leucocytes reduction that the Fructus Psoraleae glycosides is low, high dose all can resist caused by cyclophosphamide.
The influence that embodiment 14 different Fructus Psoraleae glycosides reduce caused by cyclophosphamide normal mouse numeration of leukocyte
1. experiment purpose: investigate of the influence of different Fructus Psoraleae glycosides to the caused by cyclophosphamide leucocytes reduction.
2. experiment material
2.1 medicine and reagent: Colla Corii Asini, Dong-E donkey-hide Gelatin Co., Ltd., Shandong Prov., lot number: 0311101; Cyclophosphamide for injection (CTX), Hengrui Medicine Co., Ltd., Jiangsu Prov., lot number: 07063021; The leukocyte diluent: glacial acetic acid 2ml, 1% Gentian Violet 1ml, distilled water adds to 100ml.
2.2 experiment equipment: cell counting count board, microscope, 1000 μ l, 100 μ l micropipettors.
2.3 laboratory animal
The ICR mice, male and female double, body weight 18-22g is provided by the Zhejiang Academy of Medical Sciences animal center.
3. method and step
3.1 get healthy 18-22g ICR mice, under this laboratory condition, raise after 2 days random packet:
Normal control group: isometric(al) normal saline
Model group: isometric(al) normal saline
Colla Corii Asini group: 1.5gKg
-1
Experimental group: low, high dose group (0.1gKg
-1, 0.3gKg
-1);
Every group 10.Each organized the mice successive administration 8 days.
3.2 administration the 3rd day, every mouse peritoneal injection cyclophosphamide 100mg/kg (0.2ml/10g).
3.3 behind the 8th day (injection cyclophosphamide after the 5th day) administration 2h, the eye socket vein is got blood, draws 20 μ l blood, changes 380 μ l leukocyte diluents immediately over to, shakes gently 1～2 minute, fully counts behind the mixing.
4. experimental result
Experimental result shows, behind the injection cyclophosphamide the 5th day, the model group numeration of leukocyte was (3.33 ± 0.98) * 10
6/ ml is than normal group (7.24 ± 1.62) * 10
6/ ml obviously reduces, difference significance (P＜0.01).Low dose group and high dose group numeration of leukocyte are (4.01 ± 1.02) * 10
6/ ml, (3.89 ± 0.65) * 10
6/ ml obviously raises than model group, difference significance (P＜0.05, P＜0.05).Positive controls (Colla Corii Asini group) numeration of leukocyte is (4.05 ± 0.83) * 10
6/ ml obviously raises than model group, difference significance (P＜0.05).
The influence that the different Fructus Psoraleae glycosides of table 3 reduces caused by cyclophosphamide normal mouse numeration of leukocyte (x ± s, n=10)
Annotate: compare with normal group
*P＜0.05,
*P＜0.01;
Compare with model group
△P＜0.05,
△ △P＜0.01.
5. conclusion
The leucocytes reduction that different Fructus Psoraleae glycosides is low, high dose all can resist caused by cyclophosphamide.
Claims (6)
1. the preparation method of a malaytea scurfpea fruit total glycosides extract is characterized in that being achieved through the following technical solutions:
(1) Fructus Psoraleae is solvent extraction with water,
(2) extracting solution concentrates the back precipitate with ethanol,
(3) the precipitate with ethanol supernatant extracts with little polarity organic reagent,
(4) extraction back water layer purification by macroporous resin, first water flushing, eluent is collected in the flushing of reuse ethanol water, and eluent is evaporated to does not have alcohol, and lyophilization promptly gets malaytea scurfpea fruit total glycosides extract;
In the described step (1), solvent load 8-15 doubly measures, heating extraction 1-3 time, each 0.5-2 hour;
The used concentration of alcohol of precipitate with ethanol is 70-95% in the described step (2);
The medium and small polarity organic reagent of described step (3) is selected a kind of in petroleum ether, chloroform or the ethyl acetate for use;
The determining alcohol of ethanol water is 30-100% in the described step (4), carries out assay by HPLC, and containing percentage by weight in the malaytea scurfpea fruit total glycosides is the Fructus Psoraleae glycosides of 40-50% and the different Fructus Psoraleae glycosides of 40-50%.
2. the preparation method of a kind of malaytea scurfpea fruit total glycosides extract according to claim 1, it is characterized in that: the described solvent load of step (1) is 10 times of amounts, heating extraction 2 times, each 2 hours.
3. the preparation method of a kind of malaytea scurfpea fruit total glycosides extract according to claim 1, it is characterized in that: the described precipitate with ethanol concentration of alcohol of step (2) is 80%.
4. use in the medicine of preparation leukocyte increasing according to the malaytea scurfpea fruit total glycosides extract of the described method preparation of claim 1.
5. the application of a kind of malaytea scurfpea fruit total glycosides extract according to claim 4 is characterized in that: drug prepared also contains preparation allowable pharmaceutical excipients or dressing.
6. the application of a kind of malaytea scurfpea fruit total glycosides extract according to claim 5 is characterized in that: described pharmaceutical dosage forms comprises tablet, capsule, granule, oral liquid, slow releasing preparation, controlled release preparation, gel, ointment, ointment, cream, suppository, injection, injectable powder, patch, drop pill or suspensoid.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107121502A (en) * | 2016-02-25 | 2017-09-01 | 天津中医药大学 | A kind of method for determining index components content in red school graduates pretty young woman's piece |
| CN110146600A (en) * | 2018-02-13 | 2019-08-20 | 国药集团同济堂（贵州）制药有限公司 | The preparation method and its detection method of psoralea corylifolia standard decoction |
| CN110638857A (en) * | 2019-09-25 | 2020-01-03 | 河南中医药大学 | Preparation method of fructus psoraleae total glycoside extract |
| CN111840360A (en) * | 2020-04-14 | 2020-10-30 | 江苏省中医药研究院 | New use of fructus Psoraleae total glycosides extract for treating osteoporosis |
-
2009
- 2009-05-14 CN CN2009100987671A patent/CN101549010B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107121502A (en) * | 2016-02-25 | 2017-09-01 | 天津中医药大学 | A kind of method for determining index components content in red school graduates pretty young woman's piece |
| CN110146600A (en) * | 2018-02-13 | 2019-08-20 | 国药集团同济堂（贵州）制药有限公司 | The preparation method and its detection method of psoralea corylifolia standard decoction |
| CN110146600B (en) * | 2018-02-13 | 2022-04-26 | 国药集团同济堂（贵州）制药有限公司 | Preparation method and detection method of fructus psoraleae standard decoction |
| CN110638857A (en) * | 2019-09-25 | 2020-01-03 | 河南中医药大学 | Preparation method of fructus psoraleae total glycoside extract |
| CN111840360A (en) * | 2020-04-14 | 2020-10-30 | 江苏省中医药研究院 | New use of fructus Psoraleae total glycosides extract for treating osteoporosis |
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