CN102285875A - Method for extracting and purifying isoliquiritigenin from liquorice - Google Patents
Method for extracting and purifying isoliquiritigenin from liquorice Download PDFInfo
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Abstract
The invention discloses a method for extracting and purifying isoliquiritigenin from liquorice. The method comprises the following steps of: (1) crushing the liquorice, performing continuous countercurrent extraction by using ethanol aqueous solution and filtering to obtain liquorice extract; (2) volatilizing the ethanol, adding 1mol/L hydrochloric acid solution to perform acidolysis for 2 hours, adjusting the pH value of the solution to be 12 by using sodium hydroxide, performing alkaline hydrolysis for 2 hours, and then adjusting the pH value to be 7 by using the 1mol/L hydrochloric acid solution to obtain isoliquiritigenin rough extract; and (3) filling the isoliquiritigenin rough extract in a mixed resin column, and performing elution, separation, purification and recrystallization. The isoliquiritigenin which is extracted by the method is a natural extract and has the advantages of high purity, low cost, high production efficiency and stable and controllable quality.
Description
Technical field
The invention belongs to the extracting and purifying method of isoliquiritigenin, particularly a kind of method of from Radix Glycyrrhizae, extracting the purifying isoliquiritigenin.
Background technology
Radix Glycyrrhizae is the dry root and rhizome H J that glycyrrhizic legume belongs to Glycyrrhiza uralensis Fisch. (Glycyrrhiza uralensis Fisch.), glycyrrhiza inflate bat (G.inflata Bata1.), glycyrrhiza glabra (G.glabra L.), have invigorate the spleen and benefit qi, the effect of clearing heat and detoxicating, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.Flavones ingredient has multiple biological activity in the Radix Glycyrrhizae, except effects such as anti-inflammatory, antibiotic, resistance attitude, at anti-oxidant, anticancer, anti-cancer, preventing tumor etc. tangible effect is arranged also.Modern pharmacological research shows that liquirtin, isoliquiritin, Liquiritigenin, isoliquiritigenin are the main effective constituent of licoflavone class.
Isoliquiritigenin (Isoliquiritigenin) is the isomers of Liquiritigenin, yellow crystal, and molecular formula: C15H12O4, molecular weight: 256.25, dissolve in organic solvents such as methyl alcohol, ethanol, n-propyl alcohol, Virahol, fusing point: 206 ℃～210 ℃.Modern study shows, effect such as that isoliquiritigenin has is anti-oxidant, antiulcer agent, treatment depressive psychopathia, acquired immune deficiency syndrome (AIDS), adjuvant therapy of diabetes.
The isoliquiritigenin structural formula
Continuous Countercurrent Extraction is meant in the process of extracting, the movement continuously simultaneously of material and solvent, but direction of motion is opposite, and by mechanical transfer, continuous and quantitative is reinforced, and material is fully contacted with solvent, and the equipment internal solvent is brought in constant renewal in, and finally slags tap continuously.Bring in constant renewal in solvent at device interior simultaneously, solvent constantly obtains the effective ingredient of material in flow process, and concentration improves constantly.At continuous feed liquor with continuously in the process of fluid, there is the successive concentration gradient in the solvent, thereby makes extracting solution can obtain also can obtain to have saved the extraction solvent simultaneously than higher extract concentration than leaching velocity faster.
Use the mixing of polyamide resin, two kinds of resins of macroporous resin, make isoliquiritigenin separation and purification better effects if.
At present, isoliquiritigenin extracts 1 patent, Chinese patent CN101328115 extracts the method for isoliquiritigenin from Radix Glycyrrhizae, be to use earlier acid hydrolysis, alkaline hydrolysis then, alcohol extracting, and its final purification separation also is to use single resin, and does not have recrystallization process, and it is many to consume organic solvent in the leaching process, efficient is poor, and content is low.
Summary of the invention
The objective of the invention is to make full use of the natural resources, a kind of method of extracting the purifying isoliquiritigenin from Radix Glycyrrhizae is provided.
Technical scheme of the present invention is summarized as follows:
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:10～30, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, deionized water with 5-8 times of column volume, the concentration expressed in percentage by volume of 5 times of column volumes is 20%～40% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, inhales and removes supernatant liquor, and crystallization adds the anhydrous alcohol solution of 4 times of volumes, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filter, crystallization is washed till with deionized water does not have the alcohol flavor, and drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Described hybrid resin is the mixing of polyamide resin, two kinds of resins of macroporous resin.
Described solid-liquid ratio is 1:10～30.
Described hybrid resin is the mixing of polyamide resin, two kinds of resins of silica gel, and mixing quality is than being 5:5.
Described polyamide resin model is: the 30-60 order; The 60-100 order.
Described macroporous resin, model is: HPD-100; D101B; HP-30.
Embodiment
Extract the method for purifying isoliquiritigenin below by specific embodiment explanation the present invention from Radix Glycyrrhizae, following description only is in order to make those skilled in the art understand the present invention better, but does not limit the present invention in any way.
Embodiment 1
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:10, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 30-60 order and the macroporous resin HPD-100 of 5:5 forms by mass ratio, deionized water with 5 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 20% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 96.3%.
Embodiment 2
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:20, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 60-100 order and the macroporous resin D101B of 5:5 forms by mass ratio, deionized water with 6 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 30% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 97.5%.
Embodiment 3
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:30, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 60-100 order and the macroporous resin HP-30 of 5:5 forms by mass ratio, deionized water with 7 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 40% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 98.2%.
Embodiment 4
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:25, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 30-60 order and the macroporous resin D101B of 5:5 forms by mass ratio, deionized water with 8 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 40% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 98.6%.
Embodiment 5
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:10, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 60-100 order and the macroporous resin HPD-100 of 5:5 forms by mass ratio, deionized water with 8 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 35% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 99.1%.
Embodiment 6
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:30, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 30-60 order and the macroporous resin HP-30 of 5:5 forms by mass ratio, deionized water with 7 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 25% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 96.8%.
Embodiment 7
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:15, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 60-100 order and the macroporous resin HP-30 of 5:5 forms by mass ratio, deionized water with 5 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 30% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 97.7%.
Embodiment 8
From Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, comprise the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:20, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, hybrid resin is that polyamide resin 30-60 order and the macroporous resin D101B of 5:5 forms by mass ratio, deionized water with 7 times of column volumes, the concentration expressed in percentage by volume of 5 times of column volumes is 20% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, supernatant liquor is removed in suction, crystallization adds the anhydrous alcohol solution of 4 times of volumes, is evaporated to pol 30%, adds isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filters, and crystallization is washed till with deionized water does not have the alcohol flavor, drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.Isoliquiritigenin content is 97.6%.
Claims (6)
1. from Radix Glycyrrhizae, extract the method for purifying isoliquiritigenin, it is characterized in that comprising the steps:
(1) licorice powder being broken into 60 order powder, is that 80% aqueous ethanolic solution is to extract solvent with concentration expressed in percentage by volume, adopts the Continuous Countercurrent Extraction technology, and at 50 ℃, the feed liquid mass ratio is under the condition of 1:10～30, extracts 60 minutes, filters, and gets licorice extract;
(2) gained extracting solution concentrating under reduced pressure is waved most ethanol after, the concentration that adds 8 times of volumes is the hydrochloric acid soln of 1mol/L, at 90 ℃, after the acidolysis 2 hours, use sodium hydrate regulator solution pH value to 12 then, at 90 ℃, alkaline hydrolysis 2 hours, transferring pH value with the 1mol/L hydrochloric acid soln then is 7, gets the isoliquiritigenin crude extract;
(3) with the isoliquiritigenin crude extract, last hybrid resin post, deionized water with 5-8 times of column volume, the concentration expressed in percentage by volume of 5 times of column volumes is 20%～40% aqueous ethanolic solution, the concentration expressed in percentage by volume of 4 times of column volumes is that 80% aqueous ethanolic solution carries out wash-out, the collected volume percentage concentration is 80% aqueous ethanolic solution wash-out liquid, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, inhales and removes supernatant liquor, and crystallization adds the anhydrous alcohol solution of 4 times of volumes, be evaporated to pol 30%, add isopyknic deionized water, stir evenly, crystallization is 8 hours under 20 ℃ of conditions, filter, crystallization is washed till with deionized water does not have the alcohol flavor, and drying under reduced pressure under 60 ℃ of conditions promptly obtains the isoliquiritigenin behind the purifying.
2. described hybrid resin is the mixing of polyamide resin, two kinds of resins of macroporous resin.
3. according to the described method of from Radix Glycyrrhizae, extracting the purifying isoliquiritigenin of claim 1, it is characterized in that described solid-liquid ratio is 1:10～30.
4. according to the described method of extracting the purifying isoliquiritigenin from Radix Glycyrrhizae of claim 1, it is characterized in that described hybrid resin is the mixing of polyamide resin, two kinds of resins of macroporous resin, mixing quality is than being 5:5.
5. according to claim 1 or the 3 described methods of from Radix Glycyrrhizae, extracting the purifying isoliquiritigenin, it is characterized in that described polyamide resin model is: the 30-60 order; The 60-100 order.
6. according to claim 1 or the 3 described methods of extracting the purifying isoliquiritigenin from Radix Glycyrrhizae, it is characterized in that described macroporous resin, model is: HPD-100; D101B; HP-30.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633895A (en) * | 2012-04-19 | 2012-08-15 | 南京中医药大学 | Extraction and preparation method by comprehensively utilizing liquorice |
| CN104069160A (en) * | 2014-07-17 | 2014-10-01 | 新疆鹏弘生物科技有限公司 | Ultrasonic countercurrent extraction process for glycyrrhiza |
| CN110090469A (en) * | 2019-04-26 | 2019-08-06 | 青岛农业大学 | A method of extraction purification glycyrrhizin and enoxolone from Radix Glycyrrhizae |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101073595A (en) * | 2007-06-18 | 2007-11-21 | 石任兵 | Glycyrrhiza total flavonoid and total saponin extract and its production |
| CN101328115A (en) * | 2008-07-30 | 2008-12-24 | 韩博 | Method for extracting isoliquirtigenin from licorice |
-
2011
- 2011-09-26 CN CN2011102878375A patent/CN102285875A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101073595A (en) * | 2007-06-18 | 2007-11-21 | 石任兵 | Glycyrrhiza total flavonoid and total saponin extract and its production |
| CN101328115A (en) * | 2008-07-30 | 2008-12-24 | 韩博 | Method for extracting isoliquirtigenin from licorice |
Non-Patent Citations (2)
| Title |
|---|
| 任荣军: "大孔树脂吸附技术在黄酮类成分分离纯化中的应用", 《中国药业》 * |
| 张爱华等: "甘草与附子配伍煎液的甘草黄酮含量测定", 《中成药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633895A (en) * | 2012-04-19 | 2012-08-15 | 南京中医药大学 | Extraction and preparation method by comprehensively utilizing liquorice |
| CN102633895B (en) * | 2012-04-19 | 2014-02-12 | 南京中医药大学 | Extraction and preparation method by comprehensively utilizing liquorice |
| CN104069160A (en) * | 2014-07-17 | 2014-10-01 | 新疆鹏弘生物科技有限公司 | Ultrasonic countercurrent extraction process for glycyrrhiza |
| CN110090469A (en) * | 2019-04-26 | 2019-08-06 | 青岛农业大学 | A method of extraction purification glycyrrhizin and enoxolone from Radix Glycyrrhizae |
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Application publication date: 20111221 |