CN110637763A - Preparation method of tridacna shell morphology and mantle color character interchange strain - Google Patents
Preparation method of tridacna shell morphology and mantle color character interchange strain Download PDFInfo
- Publication number
- CN110637763A CN110637763A CN201910917434.0A CN201910917434A CN110637763A CN 110637763 A CN110637763 A CN 110637763A CN 201910917434 A CN201910917434 A CN 201910917434A CN 110637763 A CN110637763 A CN 110637763A
- Authority
- CN
- China
- Prior art keywords
- tridacna
- ovum
- species
- eggs
- sperm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000512272 Tridacna Species 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title abstract description 5
- 235000013601 eggs Nutrition 0.000 claims abstract description 27
- 241000894007 species Species 0.000 claims abstract description 27
- 238000009395 breeding Methods 0.000 claims abstract description 16
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 11
- 230000002068 genetic effect Effects 0.000 claims abstract description 7
- 230000005070 ripening Effects 0.000 claims abstract description 7
- 238000009331 sowing Methods 0.000 claims abstract description 7
- 230000009027 insemination Effects 0.000 claims abstract description 6
- 230000001568 sexual effect Effects 0.000 claims abstract description 4
- 210000004681 ovum Anatomy 0.000 claims description 22
- 108010000912 Egg Proteins Proteins 0.000 claims description 18
- 102000002322 Egg Proteins Human genes 0.000 claims description 18
- 235000015170 shellfish Nutrition 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 239000013535 sea water Substances 0.000 claims description 5
- 230000002196 ecbolic effect Effects 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 206010034719 Personality change Diseases 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 230000004927 fusion Effects 0.000 claims description 3
- 230000010196 hermaphroditism Effects 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 229940076279 serotonin Drugs 0.000 claims description 3
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 2
- 101800000989 Oxytocin Proteins 0.000 claims description 2
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims description 2
- 230000010165 autogamy Effects 0.000 claims description 2
- 238000011109 contamination Methods 0.000 claims description 2
- 238000005336 cracking Methods 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims description 2
- 230000009191 jumping Effects 0.000 claims description 2
- 239000003147 molecular marker Substances 0.000 claims description 2
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims description 2
- 229960001723 oxytocin Drugs 0.000 claims description 2
- 210000000582 semen Anatomy 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims description 2
- 241001480537 Fomitopsis Species 0.000 claims 1
- 230000008030 elimination Effects 0.000 claims 1
- 238000003379 elimination reaction Methods 0.000 claims 1
- 235000020639 clam Nutrition 0.000 abstract description 45
- 241000237519 Bivalvia Species 0.000 abstract description 23
- 238000009396 hybridization Methods 0.000 abstract description 8
- 238000009403 interspecific hybridization Methods 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000035755 proliferation Effects 0.000 abstract description 4
- 241001600155 Tridacna crocea Species 0.000 description 8
- 235000014653 Carica parviflora Nutrition 0.000 description 4
- 241000243321 Cnidaria Species 0.000 description 4
- 241000596148 Crocus Species 0.000 description 4
- 235000004237 Crocus Nutrition 0.000 description 4
- 241000237852 Mollusca Species 0.000 description 4
- 230000000366 juvenile effect Effects 0.000 description 4
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 4
- 241000237503 Pectinidae Species 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 235000020637 scallop Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000237891 Haliotidae Species 0.000 description 2
- 241000237502 Ostreidae Species 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000012214 genetic breeding Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000020636 oyster Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 241001519451 Abramis brama Species 0.000 description 1
- 241000881711 Acipenser sturio Species 0.000 description 1
- 241000258920 Chilopoda Species 0.000 description 1
- 241001247319 Cuora Species 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 241001489138 Haliotis diversicolor Species 0.000 description 1
- 241001600175 Hippopus Species 0.000 description 1
- 241000556189 Huso Species 0.000 description 1
- 108091092878 Microsatellite Proteins 0.000 description 1
- 241000202002 Porcellana Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241001600160 Tridacna derasa Species 0.000 description 1
- 241001600163 Tridacna gigas Species 0.000 description 1
- 241001600159 Tridacna maxima Species 0.000 description 1
- 241000637221 Tridacna noae Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000009189 diving Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of tridacna shell shape and mantle color character interchangeable strain. It comprises the following steps of parent selection: selecting different species of tridacna as parents, and removing natural hybrid individuals to ensure the pureness of the species; parent ripening: the parents are promoted to mature in a micro-flow manner, in 4-6 months per year, the sexual maturity season of tridacna enters the breeding season; separating sperms and eggs, artificial insemination and genetic identification. According to the invention, in order to obtain the excellent properties of giant clams of different species, an interspecific hybridization breeding technology is established, an ideal new strain with shell morphology and mantle color interchanged is obtained, an unprecedented giant clam variation type is created, a new selection is provided for the marine culture, the ornamental aquarium market and the bottom sowing proliferation of giant clams, and a technical support is provided for the culture of new species of giant clams. According to the method, the hybridization among giant clam species is realized for the first time, the strain with the shell form and mantle color exchanged is obtained, the new strain with remarkable heterosis is cultivated, and the technical support is provided for the cultivation of the novel giant clam variety.
Description
Technical Field
The invention belongs to the technical field of shellfish genetic breeding in modern marine agriculture, and particularly relates to a seed production method of tridacna shell shape and mantle color interchangeable strains.
Background
Interspecific hybridization is one of basic breeding means of animals and plants, can remarkably expand gene banks of the animals and plants, promote interspecific communication, introduce heterogenous favorable genes, create unprecedented variation types and even synthesize new species. For aquatic animals, interspecific hybridization is mainly focused on fish hybridization, and a plurality of new species and variant types thereof are cultivated, so that the development of fish industries such as hybrid sturgeon huso, hybrid carp and crucian, hybrid bream and amblycephala is effectively promoted. For marine shellfish, interspecific hybridization research focuses on species such as oysters, scallops and abalones, scholars culture a new variety of ' south China No. 1 ' oysters, a new variety of ' Qingnong black scallops, a new variety of ' Qingnong golden scallops ' and a new variety of ' Haliotis diversicolor ' abalones, and guarantee is provided for the fine breed of shellfish industry in China.
Giant clams are large tropical sea coral reef benthic shellfish and are mainly distributed in areas such as the indian ocean, the western pacific ocean and the south china sea, including coral reefs or shallower reefs near the low tide regions of countries such as southern and taiwan provinces in china, indonesia, philippines, australia, malaysia, miloney, babysia, feijia, solomon islands and palo. The most unique biological characteristic of giant clams is that the giant clams are in a nutritional mode, namely the giant clams are subjected to photosynthesis through zooxanthella symbiotic in mantle films to obtain nutrition and energy required by survival, can live without ingestion and only by sunlight, and are called as photosynthetic animals. Tridacna mantle is colorful, has various patterns and is also called as 'rose in the sea'.
In China, Tridacna is mainly distributed in sea areas such as islands in the south China, Taiwan, Hainan and the like, and comprises 8 kinds of Tridacna, namely Tridacna (Tridacna gigas), scale-free Tridacna (T.derasa), scale Tridacna (T.squmosa), long Tridacna (T.maxima), Norwa Tridacna (T.noae), safranine Tridacna (T.crocea) and trioyster (Hippophus hippopus) and Cuora Tridacna (H.porcellana) of Tridacna. Lagoon, fortunes and reef discs of island reefs of south China sea coral reefs are distribution areas of giant clams. Because the discovery of the market value of tridacna and the sea area management measures in China are lagged, especially in the nineties of the twentieth century, with the popularization of diving equipment and motor boats, giant clam resources are seriously damaged, such as tridacna close to the dead track of south sea in China, tridacna without scales is extremely rare, and other tridacna are in endangered places, so that the coral reef is in danger of resource depletion and ecological system collapse.
From 2016, the south sea oceanic research institute of Chinese academy of sciences breaks through the technologies of artificial breeding, intermediate culture and bottom sowing proliferation of giant clams at home and establishes a corresponding technical matching system. Based on the breakthrough of artificial breeding technology, the scholars also develop corresponding genetic breeding work.
The invention content is as follows:
in order to try feasibility of hybridization between giant clam species, obtain a new giant clam species hybrid strain and open up a new giant clam breeding technology, the invention provides a seed preparation method for obtaining a giant clam shell form and mantle color exchange strain.
The invention discloses a seed preparation method of a tridacna shell form and mantle color interchange strain, which is characterized by comprising the following steps:
a. parent selection: selecting different species of tridacna as parents, and removing natural hybrid individuals to ensure the pureness of the species;
b. parent ripening: micro-flowing water type ripening is adopted, and the cultivation density is 10-15kg/m2(ii) a The illumination intensity is 60-80% of the normal sunlight intensity, is in tridacna sexual maturity season in 4-6 months of each year, and enters the breeding season;
c. separating sperms and eggs: carrying out parent spawning induction by using an oxytocic, and continuing to discharge ova at intervals after the parents discharge sperms because tridacna is an animal of hermaphrodite; independently collecting sperms and eggs discharged by each individual in a parent multiple transfer mode to obtain uncontaminated sperms and eggs;
d. artificial insemination: taking uncontaminated sperm and egg as gamete source, carrying out pairing artificial insemination among different species, wherein sperm: the ratio of the number of the eggs to the number of the eggs is 30-50:1, and incubating fertilized eggs by micro-inflation; because of interspecific hybridization, the phenomena of bidirectional fertilization, unidirectional fertilization and sperm resistance can exist, and all the phenomena belong to normal phenomena;
e. and (3) progeny culture: selecting excellent hatched D-type larvae, developing larva cultivation, planting zooxanthellae, young shellfish cultivation, young shellfish intermediate breeding and bottom sowing cultivation, tracking phenotypic character changes of hybrid progeny and intraspecies self-reproduction progeny in the whole process, evaluating heterosis, and screening effective hybrid combinations as new strains;
f. genetic identification: because the phenomena of sperm pollution, larva pollution, seedling pollution and the like can occur in the hybridization process, the molecular marker is adopted to carry out genetic identification on hybrid filial generation, so that the hybrid is ensured to be true amphoteric fusion filial generation and non-single species filial generation.
Preferably, the giant clams of different species are selected as parents in the step (a), and tridacna are selected as parents.
Preferably, the giant clams of different species selected in the step (a) are taken as parents, and each giant clam depends on the size of a sexual mature individual.
Preferably, the natural hybrid individuals removed in step (a) are obtained from byssus holes and a small amount of sample is used for molecular validation. Because tridacna is an animal with hermaphrodite and is easy to cause sperm pollution in the hybridization process, hybrid offspring needs genetic identification to be scientific, otherwise the result can be seriously questioned.
Preferably, the oxytocin used in the step (c) is serotonin.
Preferably, in the step (c), the uncontaminated sperm and ovum are obtained, in order to prevent sperm contamination, the obtained semen is filtered by a mesh with a diameter of 20 μm, the filtrate is collected to be the uncontaminated sperm solution, and the ovum and impurities are arranged on a filter screen; in order to observe whether the ovum is subjected to self-fertilization or not, the ovum is placed in natural seawater for 90min, the cracked ovum is discarded, and the ovum liquid which is not cracked is selected as an ovum source;
preferably, the unpolluted sperm and egg of step (d) are used as gamete source, and the sperm with jumping ability under microscope is used as male source; soaking in seawater for 90min, discarding cracked ovum, and selecting ovum with no cracking as female source.
According to the invention, in order to obtain the excellent properties of giant clams of different species, an interspecific hybridization breeding technology is established, an ideal new strain with shell morphology and mantle color interchanged is obtained, an unprecedented giant clam variation type is created, a new selection is provided for the marine culture, the ornamental aquarium market and the bottom sowing proliferation of giant clams, and a technical support is provided for the culture of new species of giant clams. According to the method, the hybridization among giant clam species is realized for the first time, the strain with the shell form and mantle color exchanged is obtained, the new strain with remarkable heterosis is cultivated, and the technical support is provided for the cultivation of the novel giant clam variety.
Description of the drawings:
fig. 1 shows shell morphology of hybridized offspring of tridacna and tridacna crocea. A, tridacna; b, tridacna crocea; c, male parent of tridacna and male parent of tridacna; d, male parent of the crocus tridacna and male parent of the tridacna.
FIG. 2 shows the mantle color of hybridized offspring of tridacna and crocus tridacna. A, tridacna; b, tridacna crocea; c, male parent of tridacna and male parent of tridacna; d, male parent of the crocus tridacna and male parent of the tridacna.
Fig. 3 is a genetic identification diagram of tridacna, tridacna crocea and hybrid offspring thereof. Note: m is 500bp DNAmarker; 1-2, tridacna; 9-10 parts of tridacna crocea; 3-5, male parent of tridacna and male child of tridacna; 6-8 of giant clam and tridacna male filial generation.
The specific implementation mode is as follows:
the invention will be further described by taking the hybridization between tridacna and safranin species in tridacna as an example, but the invention is not limited thereto.
Example 1:
a. parent selection: 15 individuals of tridacna and tridacna crocea are collected in the sea area of a centipede branch and continental island in Tridacna of Hainan province on 3 months and 5 days in 2018; firstly, appearance tests are carried out on all individuals, species of tridacna and tridacna are primarily selected, then a small amount of samples are taken from the orifices of feet to carry out molecular identification, natural hybrid species are not found, and the pure positive of the species is ensured;
b. parent ripening: placing the parents in a culture tank of a tropical marine organism experimental station in Hainan of China academy of sciences, and performing ecological ripening on the parents by adopting a micro-flowing water feeding mode; during the period, the temperature is 26.0-28.7 ℃, the salinity is 32-33ppt, and the illumination intensity is 0-507.6 mu mol s-1m-2Namely 60-80% of the normal sunlight intensity;
c. separating sperms and eggs: in 2018, 5 and 6 days, tridacna and crocus tridacna parents are matured synchronously, and a serotonin oxytocic is injected into an mantle for oxytocic; the giant clams are firstly discharged with sperms, the duration lasts about ten minutes, the giant clams are independently collected by a 10L plastic bucket and stay for about 10 minutes, the discharge of eggs is started, in order to reduce the pollution of self sperms, the giant clams are repeatedly washed by seawater, the eggs are discharged for one time, the giant clams are transferred to a second bucket, whether the eggs are polluted or not is checked, the pollution of sperms is generated under a microscope, the giant clams are transferred to a third bucket, and the pollution of the sperms is generated under the microscope; transferring to a fourth barrel, wherein the eggs are black and round eggs without sperm pollution under a microscope, and placing the eggs for later use; obtaining uncontaminated tridacna eggs by the same method; the two kinds of tridacna ova are placed for 1.5 hours, no cleavage is found under a microscope, and the fact that the ova are not fertilized and are not polluted is proved; collecting sperms, reselecting giant clams and giant clam individuals, hastening parturition to obtain sperms, filtering the sperms by using a 20-micron mesh bolting silk net in order to prevent the sperms from being polluted by eggs, collecting filtrate, namely uncontaminated sperms, and obtaining two kinds of giant clam monomer sperms by enabling the eggs and impurities to bounce actively under a microscope;
d. artificial insemination: tridacna sperms are added into tridacna crocea ovum liquid, and the sperms are: adding tridacna sperms into tridacna oosome liquid, wherein the ratio of the tridacna sperms to the tridacna oosome liquid is 30-50: 1: matching two interspecific hybridization groups, performing micro-inflation incubation, and performing fertilization for 3 hours to count the cleavage rate;
e. and (3) progeny culture: incubating for 30 hours, counting the incubation rate, breeding incubated D-type larvae, culturing the larvae according to an artificial giant clam breeding method (grant number: ZL201610454619.9), implanting zooxanthellae, culturing juvenile mollusks, performing intermediate juvenile mollusk breeding according to a semi-closed circulating water giant clam juvenile mollusk intermediate breeding method (application number: 201910124460.8), and performing bottom sowing culture according to a small-size giant clam juvenile mollusk bottom sowing proliferation method (application number: 201711050534.5); tracking phenotypic character changes of hybrid filial generation and self-reproduction filial generation in the whole process, evaluating heterosis, and screening effective hybrid combination as a new strain; after 5-6 days in 2019, after one week of culture, tridacna male parent and pink tridacna male hybrid offspring are found to grow fast, the shell shape is similar to that of tridacna, the mantle color is consistent with that of pink tridacna, the tridacna male hybrid offspring are blue-purple, and 12 ten thousand offspring are obtained; the shapes of the safranin tridacna male shell and the pink tridacna male shell are similar to those of the safranin giant clam, the color of the mantle shows the hybridization color of the two, the purple color is shown, and 2.7 ten thousand offspring are obtained (figure 1 and figure 2);
f. genetic identification: the filial generation identification is carried out by adopting microsatellite primers (F:5'-TCTCGCCTGATCTGAGGTCG-3', R:5'-GCAGGACACATTGAACATCG-3'), and the parents are shown as a single band, while the positive and negative cross filial generation has two bands, which indicates that the filial generation is the real amphoteric fusion filial generation (figure 3).
In a word, the method breaks through the intertexture isolation barrier between giant clams, breeds giant clam male hybrid offspring with significant heterosis, shows the shell form of giant clams, and the mantle color of giant clam, obtains a new strain with the shell form and mantle color interchanged between giant clams, and provides technical support for breeding of new giant clam species.
Claims (6)
1. The seed production method of tridacna shell form and mantle color interchange strain is characterized by comprising the following steps:
a. parent selection: selecting different species of tridacna as parents, and removing natural hybrid individuals to ensure the pureness of the species;
b. parent ripening: the parent adopts micro-flow water type ripening, and the cultivation density is 10-15kg/m2(ii) a The illumination intensity is 60-80% of the normal sunlight intensity, is in tridacna sexual maturity season in 4-6 months of each year, and enters the breeding season;
c. separating sperms and eggs: carrying out parent spawning induction by using an oxytocic, and continuing to discharge ova at intervals after the parents discharge sperms because tridacna is an animal of hermaphrodite; independently collecting sperms and eggs discharged by each individual in a parent multiple transfer mode to obtain uncontaminated sperms and eggs;
d. artificial insemination: taking uncontaminated sperm and egg as gamete source, carrying out pairing artificial insemination among different species, wherein sperm: the ratio of the number of the eggs to the number of the eggs is 30-50:1, and incubating fertilized eggs by micro-inflation;
e. and (3) progeny culture: selecting excellent hatched D-type larvae, developing larva cultivation, planting zooxanthellae, young shellfish cultivation, young shellfish intermediate breeding and bottom sowing cultivation, tracking phenotypic character changes of hybrid progeny and intraspecies self-reproduction progeny in the whole process, evaluating heterosis, and screening effective hybrid combinations as new strains;
f. genetic identification: and (3) genetically identifying hybrid filial generations by adopting a molecular marker, and ensuring that the hybrid filial generations are true amphoteric fusion filial generations and non-single species filial generations.
2. The method for preparing seeds according to claim 1, wherein the different species of tridacna in step (a) is tridacna and tridacna.
3. The method for producing seed according to claim 1 or 2, wherein the elimination of natural hybrid individuals in step (a) is performed by obtaining a small amount of sample from the Fomitopsis.
4. The method for producing seed according to claim 1 or 2, wherein the oxytocin in the step (c) is serotonin.
5. The method for producing seed according to claim 1 or 2, wherein the obtaining of uncontaminated sperm and ovum in step (c) is to prevent sperm contamination, the obtained semen is filtered with 20 μm mesh, and the filtrate is collected as uncontaminated sperm solution; in order to observe whether the ovum is subjected to self-fertilization or not, the ovum is placed in natural seawater for 90min and then observed, the cracked ovum is discarded, and the ovum liquid which is not cracked is selected as the source of the ovum.
6. The method for producing seed according to claim 1 or 2, wherein the unpolluted sperm and egg of step (d) are used as gamete source, and the sperm having jumping ability under microscope is used as male source; soaking in seawater for 90min, discarding cracked ovum, and selecting ovum with no cracking as female source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910917434.0A CN110637763B (en) | 2019-09-26 | 2019-09-26 | Preparation method of tridacna shell morphology and mantle color character interchange strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910917434.0A CN110637763B (en) | 2019-09-26 | 2019-09-26 | Preparation method of tridacna shell morphology and mantle color character interchange strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110637763A true CN110637763A (en) | 2020-01-03 |
| CN110637763B CN110637763B (en) | 2021-05-04 |
Family
ID=69011385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910917434.0A Active CN110637763B (en) | 2019-09-26 | 2019-09-26 | Preparation method of tridacna shell morphology and mantle color character interchange strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110637763B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02501441A (en) * | 1987-09-28 | 1990-05-24 | ドスク，リー | How to cultivate pearls |
| CN105766726A (en) * | 2016-03-23 | 2016-07-20 | 广西大学 | Chlamys nobilis (female) and Argopecten irradians concentricus Say (male) hybrid breeding method |
| CN106035166A (en) * | 2016-06-20 | 2016-10-26 | 中国科学院南海海洋研究所 | Physical method capable of effectively avoiding self-fertilization of Tridacna sp |
| CN106165659A (en) * | 2016-06-20 | 2016-11-30 | 中国科学院南海海洋研究所 | A kind of Concha Tridacna squamosa artificial breeding method |
| CN106577413A (en) * | 2016-11-01 | 2017-04-26 | 海南大学 | Artificially induced spawning method of tridacnas |
-
2019
- 2019-09-26 CN CN201910917434.0A patent/CN110637763B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02501441A (en) * | 1987-09-28 | 1990-05-24 | ドスク，リー | How to cultivate pearls |
| CN105766726A (en) * | 2016-03-23 | 2016-07-20 | 广西大学 | Chlamys nobilis (female) and Argopecten irradians concentricus Say (male) hybrid breeding method |
| CN106035166A (en) * | 2016-06-20 | 2016-10-26 | 中国科学院南海海洋研究所 | Physical method capable of effectively avoiding self-fertilization of Tridacna sp |
| CN106165659A (en) * | 2016-06-20 | 2016-11-30 | 中国科学院南海海洋研究所 | A kind of Concha Tridacna squamosa artificial breeding method |
| CN106577413A (en) * | 2016-11-01 | 2017-04-26 | 海南大学 | Artificially induced spawning method of tridacnas |
Non-Patent Citations (2)
| Title |
|---|
| MILITZ TA 等: "Larval and early juvenile culture of two giant clam (Tridacninae) hybrids", 《AQUACULTURE》 * |
| 苏柏维: "诺亚砗磲贝与长砗磲贝生殖生物学之比较研究", 《台湾博硕士论文知识加值系统》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110637763B (en) | 2021-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102301968B (en) | Method for cultivating new variety of hybridized and backcrossed Argopecten purpuratus | |
| CN102318571B (en) | Culture method of hybrid new variety of Argopecten irradias and Argopecten purpuratus | |
| CN102301971B (en) | Method for breeding novel hybrid scallop variety of Argopecten purpuratus and Argopecten irradians irradians | |
| CN109430126B (en) | Method for cultivating new hybrid scallop variety of purple cuttlefish | |
| CN110100776A (en) | A kind of selection of low temperature resistant resistance to less salt prawn kind | |
| JPH11225609A (en) | Cultivation of pearl oyster | |
| CN114208734B (en) | Method for improving production performance of portuguese oyster triploid by integrating multiple breeding technologies | |
| CN108124801B (en) | Induction method of novel oyster variety Haoda No. 2 tetraploid | |
| CN107581115B (en) | Method for improving survival rate of tridacna from juvenile stage to juvenile stage | |
| CN112535128A (en) | Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea pacifica | |
| CN108450378B (en) | Construction method for breeding families of Schlegelia rex clams | |
| CN110637763B (en) | Preparation method of tridacna shell morphology and mantle color character interchange strain | |
| Hussain et al. | Carp Genetic Resources | |
| CN114304011B (en) | Method for cultivating young mollusks of fagopyrum | |
| CN114451335B (en) | Breeding method of ternary hybrid scallop commercial seedlings | |
| CN112136736A (en) | Breeding method of stress-resistant crassostrea gigas new variety | |
| CN108541636B (en) | Method for breeding new hybrid variety of female Arragana scallop and male Patinopecten yessoensis | |
| CN105766726A (en) | Chlamys nobilis (female) and Argopecten irradians concentricus Say (male) hybrid breeding method | |
| CN112535127A (en) | Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea hongkongensis | |
| CN108040938B (en) | Method for improving production performance of crassostrea hongkongensis triploid through parent improvement | |
| JP2000354434A (en) | Culturing pearl oyster | |
| CN113678760B (en) | Cross breeding method for artificially breeding and breeding pelteobagrus fulvidraco with disease resistance | |
| CN114342847B (en) | Seed production method for improving growth of pinctada martensii with golden yellow shell color | |
| CN115486411B (en) | Method for creating new strain of anti-herpesvirus gynogenesis silver crucian carp | |
| CN114258878B (en) | In-situ spawning induction method for tridacna parents |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: No.1119 Haibin Road, Nansha District, Guangzhou City, Guangdong Province Applicant after: SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY, CHINESE ACADEMY OF SCIENCES Address before: 510301 No. 164 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant before: SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY, CHINESE ACADEMY OF SCIENCES |
|
| CB02 | Change of applicant information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |