CN110583385A - Prevention and treatment method for dragon fruit anthracnose - Google Patents
Prevention and treatment method for dragon fruit anthracnose Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
Abstract
The invention relates to the technical field of dragon fruit anthracnose control, in particular to a dragon fruit anthracnose control method, which not only promotes the growth of branches by smearing a traditional Chinese medicine extract containing growth hormone on the branches, but also enables active ingredients in the traditional Chinese medicine extract to be absorbed through stimulation effects of ethylicin and the like, thereby improving the disease resistance of crops; the breeding of the anthrax germs is inhibited and killed from the outside by the action of the beneficial bacterium liquid, the beneficial bacterium liquid can be exerted for a long time by utilizing the adsorption action of the straws, and various pathogenic bacteria can be resisted by reasonably configuring the beneficial bacterium liquid. The dragon fruit is soaked in the myrosinase liquid at a low temperature, so that a resistant layer can be formed on the surface of the dragon fruit, the postharvest diseases of the dragon fruit are prevented, and the nutritional activity and the absorption capacity of the dragon fruit are improved.
Description
Technical Field
The invention relates to the technical field of dragon fruit anthracnose control, and particularly relates to a dragon fruit anthracnose control method.
Background
The pitaya is rich in a large amount of pulp fibers, anthocyanin, carotene, vitamin B1, vitamin B2 and the like, and is rich in mineral substances such as calcium, phosphorus, iron and the like, so that the pitaya has the effects of beautifying, health care, treatment and the like; however, the occurrence of the dragon fruit diseases is frequent, the occurrence is particularly serious in hot and humid rainy seasons, the yield and the quality are seriously influenced, and the development of the dragon fruit industry is restricted.
The anthracnose is one of main diseases of the dragon fruit, has latent infectivity and mainly harms the dragon fruit; the anthracnose caused by colletotrichum gloeosporioides has light brown and water stain-like concave scabs on the surface of a fruit at the initial stage of disease attack, the scabs are enlarged and healed mutually to form large irregular scabs, and finally, an orange sticky substance is grown at the diseased part to form a conidiospore mass; after the dragon fruit is infected by the colletotrichum truncatum, small spots which are yellow in dirt and are squashed in water and round in shape appear on the surface of the fruit at the early stage of disease attack, then the disease spots are continuously enlarged, the middle of the disease spots are black, and black small particles are distributed on the disease spots, namely conidiophores and conidia; anthracnose caused by fusarium forms round, dark yellow and water stain-like disease spots on peels, pedicles and scales during attack, and white velvet-like substances are arranged on the disease spots. In addition, researches show that pathogenic bacteria such as acrosporium bicolor, Gilberry rosella, Helminthosporium opuntii and the like can also cause diseases of the picked dragon fruits.
Patent number CN201810344567.9 discloses a method for preventing and treating dragon fruit anthracnose and stalk blight, soaking dragon fruit seeds in a coating agent, wherein the coating agent comprises the following components in parts by weight: 10-15 parts of kasugamycin, 10-15 parts of a micro-fertilizer, 0.3-0.7 part of an emulsifier, 0.2-0.7 part of a dispersant, 0.5-0.8 part of a wetting agent and 40-50 parts of deionized water, soaking dragon fruit seeds, taking out, air-drying in a ventilated place, weighing the dragon fruit seeds in real time during air-drying, and stopping air-drying when the weight of the dragon fruit seeds is reduced to 10%; immersing the dragon fruit seeds in deionized water; soaking for 2-3 hours and then taking out; but the prevention and control effect on the postharvest diseases is poor, so that the search for a green prevention and control method capable of preventing and treating the dragon fruit anthracnose disease is particularly important.
Disclosure of Invention
The invention provides a method for preventing and treating dragon fruit anthracnose for solving the technical problems.
The method is realized by the following technical scheme:
a prevention and treatment method for dragon fruit anthracnose comprises prevention management and disease incidence management, wherein the prevention management comprises the following steps:
a1 growth period management: cutting 10-15 cm of the topmost part of the branch when the branch grows to 80-100 cm high, and smearing Chinese medicinal extract containing growth hormone on the topmost part;
a2 field management: spraying beneficial bacterium liquid around the ridge, then laying straw powder, and laying a reflective film on the straw powder;
a3 post-harvest management: after picking, the pitaya is placed in myrosinase liquid for low-temperature soaking.
The traditional Chinese medicine extract containing the growth hormone consists of the following components in parts by weight: 1-3 parts of growth hormone, 30-40 parts of cortex meliae aqueous extract and 10-13 parts of pumpkin seed alcohol extract.
The preparation method of the cortex Meliae water extract comprises freezing cortex Meliae at 3-5 deg.C for 2-3h, pulping, adding 2-3 times of water, treating at 70-80 deg.C for 45-60min, and filtering to obtain filtrate as water extract.
The preparation method of the pumpkin seed alcohol extract comprises the steps of freezing pumpkin seeds for 2-3h at the temperature of 3-5 ℃, pulping, adding 2-3 times of volume of 50-60% ethanol solution, treating for 45-60min at the temperature of 40-50 ℃, and filtering to obtain filtrate, namely the alcohol extract.
The growth hormone consists of gibberellin, ethylicin and brassinosterol, wherein the mass concentration of the gibberellin is 1-4%, the mass concentration of the ethylicin is 2-5%, and the mass concentration of the brassinosterol is 1-4%.
Each liter of beneficial bacterium liquid comprises biocontrol yeast (1.5-2.5) multiplied by 107cfu, phosphate solubilizing bacteria (1.0-2.0). times.106cfu, Bacillus amyloliquefaciens (1.3-1.7). times.108cfu。
Each liter of beneficial bacterium liquid also comprises 1-3g of sodium chloride and 4-8g of sodium bicarbonate.
The granularity of the straw powder is 80-100 meshes.
The preparation method of the myrosinase liquid comprises the following steps: freezing cabbage at 3-5 deg.C for 2-3h, pulping, adding 1-1.4 times of sodium bicarbonate solution, mixing, filtering to obtain filtrate, adding 4-6 times of ethanol and 0.1-0.2% of starch into the filtrate at 3-5 deg.C, mixing, and centrifuging at 8000 ion 10000r/min for 5-8min to obtain precipitate.
The low-temperature soaking is carried out at the working temperature of 10-15 ℃ for 20-30 min.
The mass concentration of the sodium bicarbonate solution is 10-15%.
The disease management is that azoxystrobin wettable powder and Borax-manganese zinc are alternately sprayed at the initial stage of disease, and the disease is controlled for 1 time every 7 to 10 days and for 2 to 3 times in total.
The azoxystrobin wettable powder is 600-fold and 750-fold azoxystrobin wettable powder.
The Bora manganese zinc is 800-times liquid of Bora manganese zinc.
Has the advantages that:
by adopting the method, the dragon fruit anthracnose hazard rate is reduced by 15%, the commodity fruit rate is improved by 12%, the income per mu is increased by more than 600 yuan, and the prevention and treatment cost is low.
According to the invention, the branches are coated with the traditional Chinese medicine extract containing growth hormone, so that the growth of the branches is promoted, and effective components in the traditional Chinese medicine extract are absorbed under the stimulation of ethylicin and the like, so that the disease resistance of crops is improved.
According to the invention, the breeding of anthrax bacteria is inhibited and killed from the outside through the effect of the beneficial bacteria liquid, the beneficial bacteria liquid can be exerted for a long time by utilizing the adsorption effect of the straw, and various pathogenic bacteria can be resisted through reasonable configuration of the beneficial bacteria liquid.
According to the invention, the dragon fruit is soaked in the myrosinase liquid at a low temperature, so that a resistant layer can be formed on the surface of the dragon fruit, the postharvest diseases of the dragon fruit are prevented, and the nutritional activity and the absorption capacity of the dragon fruit are improved.
The invention reduces the incidence and recurrence rate of dragon fruit anthracnose by improving the disease resistance and absorption capacity of the dragon fruit and combining external defense, and simultaneously improves the effective cure rate of the dragon fruit anthracnose and reduces the cure cost.
Detailed Description
The following is a detailed description of the embodiments of the present invention, but the present invention is not limited to these embodiments, and any modifications or substitutions in the basic spirit of the embodiments are included in the scope of the present invention as claimed in the claims.
Example 1
A prevention and treatment method for dragon fruit anthracnose comprises prevention management and disease incidence management, wherein the prevention management comprises the following steps:
a1 growth period management: cutting 10-15 cm of the topmost part of the branch when the branch grows to 80-100 cm high, and smearing Chinese medicinal extract containing growth hormone on the topmost part;
a2 field management: spraying beneficial bacterium liquid on two sides of the ridge, then paving straw powder, and paving a reflective film on the straw powder;
a3 post-harvest management: after picking, the pitaya is placed in myrosinase liquid for low-temperature soaking.
The traditional Chinese medicine extract containing the growth hormone consists of the following components in parts by weight: 3 parts of growth hormone, 40 parts of chinaberry bark aqueous extract and 13 parts of pumpkin seed alcohol extract;
the preparation method of cortex Meliae water extract comprises freezing cortex Meliae at 3-5 deg.C for 3 hr, pulping, adding 3 times volume of water, treating at 80 deg.C for 60min, and filtering to obtain filtrate as water extract;
the preparation method of the pumpkin seed alcohol extract comprises the steps of freezing pumpkin seeds for 3 hours at the temperature of 3-5 ℃, pulping, adding an ethanol solution with the volume of 3 times and the mass concentration of 60%, treating for 60 minutes at the temperature of 50 ℃, and filtering to obtain filtrate, namely the alcohol extract;
the growth hormone consists of gibberellin, ethylicin and brassinosteroid, wherein the mass concentration of the gibberellin is 4%, the mass concentration of the ethylicin is 5% and the mass concentration of the brassinosteroid is 4%;
each liter of beneficial bacterium liquid comprises biocontrol yeast 2.5 multiplied by 107cfu, 2.0X 10. sup. th of phosphate solubilizing bacteria6cfu, Bacillus amyloliquefaciens 1.7X 108cfu；
Each liter of beneficial bacterium liquid also comprises 3g of sodium chloride and 8g of sodium bicarbonate;
the granularity of the straw powder is 100 meshes;
the preparation method of the myrosinase liquid comprises the following steps: freezing cabbage at 3-5 deg.C for 3h, pulping, adding 1.4 times of sodium bicarbonate solution with mass concentration of 15%, mixing, filtering to obtain filtrate, adding 6 times of ethanol and 0.2% of starch at 3-5 deg.C, mixing, and centrifuging at 10000r/min for 8min to obtain precipitate;
the working temperature of the low-temperature soaking is 15 ℃, and the time is 30 min;
the disease management is that azoxystrobin wettable powder and Borax-manganese zinc are alternately sprayed at the initial stage of disease, and the disease is controlled for 1 time every 10 days and 3 times in total;
the azoxystrobin wettable powder is 750 times of azoxystrobin wettable powder;
the Boer manganese zinc is 1000 times of the Boer manganese zinc solution.
Example 2
A prevention and treatment method for dragon fruit anthracnose comprises prevention management and disease incidence management, wherein the prevention management comprises the following steps:
a1 growth period management: cutting 10-15 cm of the topmost part of the branch when the branch grows to 80-100 cm high, and smearing Chinese medicinal extract containing growth hormone on the topmost part;
a2 field management: spraying beneficial bacterium liquid around the ridge, then laying straw powder, and laying a reflective film on the straw powder;
a3 post-harvest management: after picking, the pitaya is placed in myrosinase liquid for low-temperature soaking.
The traditional Chinese medicine extract containing the growth hormone consists of the following components in parts by weight: 1 part of growth hormone, 30 parts of chinaberry bark aqueous extract and 10 parts of pumpkin seed alcohol extract;
the preparation method of cortex Meliae water extract comprises freezing cortex Meliae at 3-5 deg.C for 2 hr, pulping, adding 2 times volume of water, treating at 70 deg.C for 45min, and filtering to obtain filtrate as water extract;
the preparation method of the pumpkin seed alcohol extract comprises the steps of freezing pumpkin seeds for 2 hours at the temperature of 3-5 ℃, pulping, adding 2 times of volume of 50% ethanol solution, treating for 45min at the temperature of 40 ℃, and filtering to obtain filtrate, namely the alcohol extract;
the growth hormone consists of gibberellin, ethylicin and brassinosteroid, wherein the mass concentration of the gibberellin is 1%, the mass concentration of the ethylicin is 2% and the mass concentration of the brassinosteroid is 1%;
each liter of beneficial bacterium liquid comprises biocontrol yeast of 1.5 multiplied by 107cfu, phosphate solubilizing bacteria 1.0X 106cfu, Bacillus amyloliquefaciens 1.3X 108cfu；
Each liter of beneficial bacterium liquid also comprises 1g of sodium chloride and 4g of sodium bicarbonate;
the granularity of the straw powder is 80 meshes;
the preparation method of the myrosinase liquid comprises the following steps: freezing cabbage at 3-5 deg.C for 2h, pulping, adding 1 volume of 10% sodium bicarbonate solution, mixing, filtering to obtain filtrate, adding 4 volume of ethanol and 0.1 volume% starch into the filtrate at 3-5 deg.C, mixing, and centrifuging at 8000r/min for 5min to obtain precipitate;
the working temperature of the low-temperature soaking is 10 ℃, and the time is 20 min;
the disease management is that azoxystrobin wettable powder and Borax-manganese zinc are alternately sprayed at the initial stage of disease, and the disease is prevented and controlled for 1 time every 7 days and for 2 times in total;
the azoxystrobin wettable powder is 600 times of azoxystrobin wettable powder;
the Boer manganese zinc is 800 times of Boer manganese zinc liquid.
Example 3
A prevention and treatment method for dragon fruit anthracnose comprises prevention management and disease incidence management, wherein the prevention management comprises the following steps:
a1 growth period management: cutting 10-15 cm of the topmost part of the branch when the branch grows to 80-100 cm high, and smearing Chinese medicinal extract containing growth hormone on the topmost part;
a2 field management: spraying beneficial bacterium liquid on two sides of the ridge, then paving straw powder, and paving a reflective film on the straw powder;
a3 post-harvest management: after picking, the pitaya is placed in myrosinase liquid for low-temperature soaking.
The traditional Chinese medicine extract containing the growth hormone consists of the following components in parts by weight: 2 parts of growth hormone, 35 parts of chinaberry bark aqueous extract and 11 parts of pumpkin seed alcohol extract;
the preparation method of the cortex Meliae water extract comprises freezing cortex Meliae at 3-5 deg.C for 2.5h, pulping, adding 2.5 times of water, treating at 75 deg.C for 50min, and filtering to obtain filtrate as water extract;
the preparation method of the pumpkin seed alcohol extract comprises freezing pumpkin seeds at 3-5 deg.C for 2.5h, pulping, adding 2.5 times volume of 55% ethanol solution, treating at 45 deg.C for 50min, and filtering to obtain filtrate as alcohol extract;
the growth hormone consists of gibberellin, ethylicin and brassinosteroid, wherein the mass concentration of the gibberellin is 2.5 percent, the mass concentration of the ethylicin is 3.5 percent, and the mass concentration of the brassinosteroid is 2.5 percent;
each liter of beneficial bacterium liquid comprises biocontrol yeast 2 multiplied by 107cfu, phosphate solubilizing bacteria 1.5X 106cfu, Bacillus amyloliquefaciens 1.5X 108cfu；
Each liter of beneficial bacterium liquid also comprises 2g of sodium chloride and 6g of sodium bicarbonate;
the granularity of the straw powder is 90 meshes;
the preparation method of the myrosinase liquid comprises the following steps: freezing cabbage at 3-5 deg.C for 2.5h, pulping, adding 1.2 times volume of 12% sodium bicarbonate solution, mixing, filtering to obtain filtrate, adding 5 times volume of ethanol and 0.15% volume of starch into the filtrate at 3-5 deg.C, mixing, and centrifuging at 9000r/min for 7min to obtain precipitate;
the working temperature of the low-temperature soaking is 12 ℃, and the time is 25 min;
the disease management is that azoxystrobin wettable powder and Borax-manganese zinc are alternately sprayed at the initial stage of disease, and the disease is prevented and controlled for 1 time every 7 days and 3 times in total;
the azoxystrobin wettable powder is 750 times of azoxystrobin wettable powder;
the Boer manganese zinc is 800 times of Boer manganese zinc liquid.
Comparative example 1
The difference from example 3 is that: the traditional Chinese medicine extract containing the growth hormone does not contain pumpkin seeds. The composition comprises the following components in parts by weight: 2 parts of growth hormone, 35 parts of chinaberry bark aqueous extract and 11 parts of pumpkin seed alcohol extract;
the growth hormone consists of gibberellin, ethylicin and brassinosteroid, wherein the mass concentration of the gibberellin is 2.5 percent, the mass concentration of the ethylicin is 3.5 percent, and the mass concentration of the brassinosteroid is 2.5 percent;
comparative example 2
The difference from example 3 is that: ethylicin is not added in the growth hormone.
Comparative example 3
The difference from example 3 is that: brassinosteroids are not added to the growth hormone.
Comparative example 4
The difference from example 3 is that: the traditional Chinese medicine extract containing the growth hormone consists of the following components in parts by weight: 2 parts of growth hormone, 35 parts of chinaberry bark aqueous extract and 11 parts of rangooncreeper fruit alcohol extract.
Test example 1
On the basis of a traditional planting method, the dragon fruit fruits of the treatment groups are punctured on the surfaces of the fruits by using a sterilized puncher (with the diameter of 5mm) after the fruits are picked, then 20 mu L of spore suspension is respectively inoculated into the holes, the inoculated dragon fruit fruits are placed in a preservation box and stored at the temperature of 24-26 ℃ under the condition of RH 85-95%, and the diameter of the disease spot is measured after 4d, and the result is shown in Table 1;
TABLE 1
| Control group | Example 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Diameter of lesion | 11.8mm | 4.7mm | 8.6mm | 7.9mm | 8.2mm | 7.4mm |
Meanwhile, the inventor also performs an infection test on the pitaya fruit of example 3 by using various pathogenic bacteria, and the results are shown in table 2:
TABLE 2
The inventors also performed the content measurement of total phenols, flavonoids and lignin on the control group, the example 3 group and the comparative example group, and the measurement method is as follows as shown in table 3:
the method for measuring the total phenol content comprises the following steps: putting 1.0g of pitaya peel into a mortar, adding 12mL of precooled 80% methanol aqueous solution, fully grinding into homogenate, collecting into a 50mL centrifuge tube, standing at room temperature for 50min, centrifuging (4 ℃, 12000Xg, 15min), and collecting supernatant; taking a reaction tube, adding 1mL of supernatant, adding 80% methanol into a comparison tube in the same amount, adding 1mL of Folin-Ciocalteu reagent again, and mixing uniformly. After 10min, 10mL of 7% sodium carbonate was added and shaken well. After standing at room temperature in the dark for 1.5h, the OD of the reaction mixture was measured750The absorbance value of (c). In the test, gallic acid is used as a standard curve, and the content of total phenol is calculated according to the standard curve, wherein the unit is mg/g.
The method for measuring the flavonoid content comprises the following steps: using NaNO2-AlCl3The measurement was carried out by colorimetry. Taking test tubes with plug scales, adding 1mL of supernatant into the test tubes, adding 80% methanol solution into blank tubes, and adding 5% NaNO into each test tube20.5mL, shake well, stand for 5min, then add 10% AlCl30.5mL, shake well, stand for 6min, add 1mol/L sodium hydroxide 4mL, shake well. Measurement of OD of the reaction mixture510The absorbance value of (c). In the test, catechin is used as a standard curve, and the content of flavonoid is calculated according to the standard curve, wherein the unit is mg/g.
The method for measuring the lignin content comprises the following steps: putting 2.0g of pitaya peel into a mortar, adding 15mL of precooled 95% ethanol, grinding until the mixture is homogenized, placing the homogenized mixture into a 50mL centrifuge tube, centrifuging the homogenized mixture in a centrifuge (4 ℃, 12000Xg, 10min), removing supernatant, and collecting precipitate. Washing the precipitate with 15mL of distilled water, centrifuging, collecting the precipitate, washing the precipitate with 15mL of ethanol, acetone and ether in sequence, collecting the final precipitate, and drying at 70 ℃ for later use. 0.02g of dried precipitate is put into a test tube, 1mL of 25% acetyl bromide glacial acetic acid solution is added, and then water bath is carried out at 70 ℃ for 45min to dissolve the precipitate. After cooling, 2mmol/L of hydroxylamine/OH 1mL was added to terminate the reaction, and 2mL of glacial acetic acid and 0.1mL of 7.5mol/L hydroxylamine hydrochloride were added. Mixing, centrifuging to collect supernatant, collecting supernatant 1mL, adding glacial acetic acid to constant volume of 9mL, and adding into the supernatant A280Measuring the absorbance of the sample by using A280and/gFW.
TABLE 3
| Control group | Example 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Total phenols | 0.32 | 0.46 | 0.37 | 0.40 | 0.41 | 0.39 |
| Flavonoids | 0.11 | 0.25 | 0.18 | 0.21 | 0.16 | 0.20 |
| Lignin | 0.19 | 0.31 | 0.26 | 0.21 | 0.21 | 0.24 |
Test example 2 myrosinase solution effectiveness test
The inventor researches the effectiveness of the myrosinase liquid, and the experiment is as follows: the treatment groups of examples 1 to 3 were used, the control group was not managed after harvest, and then the fruit was punctured on the surface 2 days after harvest using a sterilized perforator (diameter 5mm), 20. mu.L of spore suspension was inoculated into the holes, respectively, and the inoculated pitaya fruit was placed in a preservation box and stored at 24 to 26 ℃ under RH85 to 95% for 4 days, and the lesion diameter was measured after 4 days, and the results are shown in Table 4;
TABLE 4
Meanwhile, the applicant also compared the changes in the antibacterial component content before and after 30 days of storage under low temperature conditions in the examples and the comparative examples, and the determination methods were the same as those in test example 1, as shown in table 5, respectively;
TABLE 5
Claims (10)
1. The prevention and treatment method for the dragon fruit anthracnose comprises prevention management and disease incidence management, and is characterized in that the prevention management comprises the following steps:
a1 growth period management: cutting 10-15 cm of the topmost part of the branch when the branch grows to 80-100 cm high, and smearing Chinese medicinal extract containing growth hormone on the topmost part;
a2 field management: spraying beneficial bacterium liquid around the ridge, then laying straw powder, and laying a reflective film on the straw powder;
a3 post-harvest management: after picking, the pitaya is placed in myrosinase liquid for low-temperature soaking.
2. The method for preventing and treating dragon fruit anthracnose of claim 1, wherein the traditional Chinese medicine extract containing growth hormone comprises the following components in parts by weight: 1-3 parts of growth hormone, 30-40 parts of cortex meliae aqueous extract and 10-13 parts of pumpkin seed alcohol extract.
3. The method for preventing and treating dragon fruit anthracnose of claim 2, wherein the cortex meliae aqueous extract is prepared by freezing cortex meliae at 3-5 deg.C for 2-3h, pulping, adding 2-3 times volume of water, treating at 70-80 deg.C for 45-60min, and filtering to obtain filtrate as aqueous extract.
4. The method for preventing and treating dragon fruit anthracnose as claimed in claim 2, wherein the pumpkin seed alcohol extract is prepared by freezing pumpkin seed at 3-5 deg.C for 2-3h, pulping, adding 2-3 times volume of 50-60% ethanol solution, treating at 40-50 deg.C for 45-60min, and filtering to obtain filtrate as alcohol extract.
5. The method for preventing and treating dragon fruit anthracnose according to claim 2, wherein the growth hormone consists of gibberellin, ethylicin and brassinosteroids, and the mass concentration of gibberellin is 1-4%, the mass concentration of ethylicin is 2-5% and the mass concentration of brassinosteroids is 1-4%.
6. The method for preventing and treating dragon fruit anthracnose of claim 1, wherein each liter of the beneficial bacterium solution comprises biocontrol yeast (1.5-2.5) x 107cfu, phosphate solubilizing bacteria (1.0-2.0). times.106cfu, Bacillus amyloliquefaciens (1.3-1.7). times.108cfu。
7. The method for preventing and treating dragon fruit anthracnose of claim 1, wherein each liter of the beneficial bacterium solution further comprises 1-3g of sodium chloride and 4-8g of sodium bicarbonate.
8. The method for preventing and treating dragon fruit anthracnose of claim 1, wherein the straw powder has a particle size of 80-100 mesh.
9. The method for preventing and treating dragon fruit anthracnose of claim 1, wherein the myrosinase solution is prepared by the following steps: freezing cabbage at 3-5 deg.C for 2-3h, pulping, adding 1-1.4 times of sodium bicarbonate solution, mixing, filtering to obtain filtrate, adding 4-6 times of ethanol and 0.1-0.2% of starch into the filtrate at 3-5 deg.C, mixing, and centrifuging at 8000 ion 10000r/min for 5-8min to obtain precipitate.
10. The method for preventing and treating dragon fruit anthracnose of claim 1, wherein the low-temperature soaking temperature is 10-15 ℃ and the time is 20-30 min.
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