CN110582573A - 植酸酶在黑曲霉中表达 - Google Patents

植酸酶在黑曲霉中表达 Download PDF

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CN110582573A
CN110582573A CN201880010972.5A CN201880010972A CN110582573A CN 110582573 A CN110582573 A CN 110582573A CN 201880010972 A CN201880010972 A CN 201880010972A CN 110582573 A CN110582573 A CN 110582573A
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seq
mutant
coli phytase
phytase
expression
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CN110582573B (zh
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白挨玺
李峰
卞芙蓉
朱继东
孙艳
徐红
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Nanjing Bestzyme Bio Engineering Co ltd
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Nanjing Bestzyme Bio Engineering Co ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
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    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03008—3-Phytase (3.1.3.8)
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03026—4-Phytase (3.1.3.26), i.e. 6-phytase
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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Abstract

本发明公开了使用核苷酸序列如SEQ ID NO.7所示的优化的大肠杆菌植酸酶基因以及核苷酸序列如SEQ ID NO.12所示的信号肽在丝状真菌中表达植酸酶的方法。

Description

PCT国内申请，说明书已公开。

Claims (15)

  1. PCT国内申请，权利要求书已公开。
CN201880010972.5A 2017-03-30 2018-03-30 植酸酶在黑曲霉中表达 Active CN110582573B (zh)

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CN201710203473 2017-03-30
CN2017107419630 2017-08-25
CN201710741963.0A CN107353327A (zh) 2017-03-30 2017-08-25 植酸酶在黑曲霉中表达
PCT/CN2018/081272 WO2018177402A1 (zh) 2017-03-30 2018-03-30 植酸酶在黑曲霉中表达

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Publication number Priority date Publication date Assignee Title
CN112204136B (zh) * 2018-05-30 2024-05-14 南京百斯杰生物工程有限公司 植酸酶突变体
CN112852800B (zh) * 2020-01-09 2023-02-03 中国科学院天津工业生物技术研究所 一种信号肽突变体及其应用
CN117683745A (zh) * 2020-04-29 2024-03-12 南京百斯杰生物工程有限公司 一种亲本植酸酶变体
CN113736672A (zh) * 2021-09-27 2021-12-03 南京正扬生物科技有限公司 一种能够大量表达南极假丝酵母脂肪酶b的黑曲霉重组菌株及其构建方法及应用

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US11339397B2 (en) 2022-05-24
CN110582573B (zh) 2023-10-31
JP7160829B2 (ja) 2022-10-25
US20210032637A1 (en) 2021-02-04
EP3604542A1 (en) 2020-02-05
EP3604542A4 (en) 2021-01-13
CN107353327A (zh) 2017-11-17
JP2020511995A (ja) 2020-04-23
WO2018177402A1 (zh) 2018-10-04

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