CN110558339B

CN110558339B - Inhibitor for masson pine growth based on Grignard tannin extract litter leaching solution

Info

Publication number: CN110558339B
Application number: CN201910997578.1A
Authority: CN
Inventors: 何中声; 晋梦然; 王哲; 邢聪; 朱静; 王雪琳; 贾梅花; 刘金福
Original assignee: Fujian Agriculture and Forestry University
Current assignee: Fujian Agriculture and Forestry University
Priority date: 2019-10-21
Filing date: 2019-10-21
Publication date: 2021-04-27
Anticipated expiration: 2039-10-21
Also published as: CN110558339A

Abstract

The invention discloses an inhibitor for masson pine growth based on a leaching liquor of a castanospermia griffithii extract, belonging to the technical field of plant protection. The inhibitor is prepared by mixing a leaching liquor stock solution of a castration of a graham tannin extract with distilled water according to a certain proportion. The preparation method of the leaching liquor stock solution of the castanopsis gracilis litter comprises the following steps: crushing the castellated tannin extract, adding distilled water according to a certain proportion, soaking for a period of time, filtering by using clean gauze, and finally carrying out suction filtration on the filtrate by using a vacuum pump to obtain a castellated tannin extract liquid stock solution. The masson pine growth inhibitor has the advantages of simple and easily obtained raw materials, no environmental pollution and simple, convenient and quick preparation method. The inhibitor has a remarkable inhibiting effect on the germination of pinus massoniana seeds. The inhibitor of the invention has important guiding significance for protecting the rare tree species community from the chemical effect perspective to implement the protection of the Grignard tannin extract.

Description

Inhibitor for masson pine growth based on Grignard tannin extract litter leaching solution

Technical Field

The invention belongs to the technical field of plant protection, and particularly relates to an inhibitor for masson pine growth based on a leaching liquor of a castanopsis gracilis litter.

Background

Allelopathic effect (allelopathiy) refers to the promotion or inhibition of seed germination or seedling growth by a plant, or other plants, by the release of chemicals into the environment. Allelochemicals are widely used in forest ecosystems, and constitute interactions among plants together with competition among light, water, nutrients and spaces, and have important influence on the structure and the function of forest communities, wherein the interaction media are secondary chemicals, called allelochemicals, mainly existing in various organs of plants, such as roots, stems, leaves, flowers and fruits.

Extract of Grignard tannin (A)Castanopsis kawakamii) Is evergreen tall trees of the tannin of fagaceae, and is only distributed in West province of Fujian, Taiwan, Jiangxi, Guangdong, Guangxi and the like in China as a third period of wiggle plant. At present, the group building species of the natural tannin extract forest of the Grignard tannin extract is in decline, the population quantity of the first generation is small, the natural updating of seedlings under the forest is poor, and the forest margin is associated with the species pinus massoniana (A)Pinus massoniana) And (b) a wood lotusSchima superba) And fir (Cunninghamia lanceolata) And the like, so that the growth of accompanying species is inhibited, and the protection of the rare germplasm resources of the castanosis gracilis becomes urgent. The natural litters of the grazing tannin extract have large quantity and high tannin and total phenol content, and the allelopathy of the litters is utilized to inhibit the growth of associated species, so the method is simple and easy to implement and has no pollution to the environment.

Disclosure of Invention

The invention aims to provide an inhibitor for the growth of masson pine based on a leaching solution of a castanopsis gracilis litter. The masson pine growth inhibitor provided by the invention has the advantages of easily available raw materials, simple and convenient preparation method, no environmental pollution and good inhibition effect on the masson pine growth.

In order to achieve the purpose, the invention adopts the following technical scheme:

a Chinese medicinal composition for inhibiting the growth of masson pine based on the extract of litters of Grignard tannin extracts is prepared from the raw extract of litters of Grignard tannin extracts and distilled water through proportional mixing.

The preparation method of the leaching liquor stock solution of the castanopsis gracilis litter comprises the following steps: crushing the castellated tannin extract, adding distilled water according to a certain proportion, soaking for a period of time, filtering by using clean gauze, and finally carrying out suction filtration on the filtrate by using a vacuum pump to obtain a castellated tannin extract liquid stock solution.

Preferably, the castellated extract of the Girardinia gracilis is selected from the group consisting of branches, leaves, skins and shells of the Girardinia gracilis.

Preferably, the castration of the tannin extract is crushed to a size of 1-2 cm.

Preferably, 1 g.5 mL^-1To the castration of tannin extract, distilled water was added.

Preferably, the litters of the tannin extract are soaked in distilled water for 48 hours at normal temperature.

The volume ratio of the mixed extract liquor of the castanopsis gracilis litter to the distilled water is as follows: 1:10, 1:30, 1:50, 1: 100.

Preferably, the extract liquor based on the castanosoma griseum litters inhibits the growth of masson pine, and consists of castanosoma griseum litters extract stock liquor.

An inhibitor for the growth of masson pine based on the leaching liquor of the castanopsis gracilis litter is applied to inhibit the germination of masson pine seeds.

The invention has the advantages that:

the invention provides an inhibitor for masson pine growth based on a leaching liquor of a castanopsis gracilis litter. The inhibitor is prepared by mixing the leaching liquor stock solution of the castration extract of the tannin extract with distilled water, and has the advantages of simple and easily obtained raw materials, no environmental pollution and simple and rapid preparation method. The inhibitor has a remarkable inhibiting effect on the germination of pinus massoniana seeds. The inhibitor of the invention has important guiding significance for protecting the rare tree species community from the chemical effect perspective to implement the protection of the Grignard tannin extract.

Drawings

FIG. 1: influence of different concentrations of the litter leaching liquor on the growth of the radicles of the pinus massoniana seeds. A: a Grignard tannin extract; b: a Grignard tannin extract; c; a Grignard tannin extract; d: the extract of the leaf of the Kalanchoe extract.

Detailed Description

In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the following examples are only examples of the present invention and do not represent the scope of the present invention defined by the claims.

Example 1

(1) Material selection

Collecting different types of litters in a fixed sample plot in a Fujian Sanming tannin extract protection area in 2018, wherein the litters are divided into 4 types of branches, leaves, skins and shells, and each type of litter is 4 Kg. Selecting the second generation pinus massoniana Linn fine seeds of the Longyan Zhangping national forest farm, wherein the total amount of the fine seeds is 1 Kg.

(2) Preparation of leach liquors

Pulverizing collected tannin extract branches, leaves, peels and shell litter to 1-2cm, and making into powder of 1 g.5 mL^-1Adding a proper amount of distilled water into 1 g of dry matter according to a proportion, soaking for 48 hours at normal temperature, filtering by using clean gauze, and finally carrying out vacuum filtration on the filtrate by using a vacuum pump to obtain an extraction stock solution, mixing the stock solution and the distilled water according to a volume ratio of 1:10, 1:30, 1:50 and 1:100 in consideration of leaching decomposition effect of litter in a natural state, preparing four kinds of leaching solutions with different concentrations respectively, and placing the four kinds of leaching solutions into a refrigerator at 4 ℃ for later use.

(3) Seed germination experiment

Selecting masson pine seeds with uniform size and full particles, washing the masson pine seeds with distilled water for several times, and adding KMnO with the effective mass fraction of 0.5%₄Sterilizing the solution for 20 min, repeatedly washing with distilled water for 3 times, soaking in distilled water for 24 hr, and air drying to remove water. The germination bed consists of quantitative filter paper with the diameter of 15 cm and a culture dish, and the culture dish is wiped and disinfected by absolute alcohol before an experiment. Taking 5mL of leaching liquor with different concentrations and types, respectively adding into culture dishes paved with double-layer filter paper, uniformly placing 100 disinfected seeds in each culture dish, repeating for 3 times at each concentration, and adding equivalent distilled water for treatment to serve as a control. Placing the culture dish in an artificial incubator with photoperiod of 14 h, 25 deg.C, dark period of 12h, 20 deg.C, and light intensity of 50 nmol cm^-2·s^-1And the relative humidity is 75-80%. Equal amount (1-5 mL) of leaching liquor with corresponding concentration is supplemented every day to keep the filter paper moist.

(4) Determination of germination and morphological indices

Setting the seeds on the day 1 d, observing and recording the number of the germinated seeds every day, and finishing the germination experiment when no seeds germinate for 3 d continuously by taking the radicle exposed 1-2mm as a standard. And (3) selecting 20 seeds with basically consistent growth vigor from culture dishes with different concentrations in the full germination period of the seeds, measuring and recording the length of the radicle every two days, and finally calculating the germination rate, the germination vigor and the germination index.

Germination rate (%) = (number of seeds to germinate/total number of seeds to be tested) × 100%;

germination vigor (%) = (number of seeds normally germinated up to the highest day of average germination/total number of test seeds) × 100%;

germination index = ∑ (Gt/Dt）

Wherein:Dtthe number of days of germination is referred to,Gtfinger andDtcorresponding number of germinated seeds per day.

The research results are as follows:

the results of the effect of different concentrations and species of the leaching solution on the germination of masson pine seeds are shown in table 1. Table 1 the results show that: the germination rate of the masson pine seeds of the control group is 77.75 percent; after the masson pine seeds are respectively treated by leaching liquor of different types and different concentrations (stock solution, 1:10, 1:30, 1:50 and 1: 100) of shells, branches, leaves and barks, the highest germination rate is 1:10 shells (90.36%), stock solution barks (95.87%), stock solution and 1:50 branches (81.33%) and 1:100 leaves (84.00%); the germination rate was the smallest at 1:50 shells (80.08%), 1:100 peels (71.62%), 1:100 shoots (77.67%), 1:50 leaves (76.67%). The germination potential of the pinus massoniana seeds in the control group is 54.75 percent, the maximum germination potential of the treatment group is 1:100 shells (58.10 percent), stock solution peel (60.97 percent), 1:50 branches (52.67 percent) and 1:100 leaves (61.33 percent); the minimal germination potential was the original liquid shell (47.69%), 1:30 bark (40.88%), 1:5 shoots (39.33%), 1:5 leaves (47.00%). The germination index of the masson pine seeds of the control group is 95.32; the germination index is maximum 1:100 shells (98.35), stock solution skin (101.66), 1:50 branches (99.86) and 1:100 leaves (109.88); the smallest germination index was 1:50 husks (85.33), 1:30 husks (71.83), 1:10 branches (82.85), 1:10 leaves (92.57%).

TABLE 1 Effect of different concentrations and species of leach liquors on the germination of Pinus massoniana seeds

The effect of various concentrations of litter leachate on the growth of pinus massoniana radicle is shown in figure 1. The results in FIG. 1 show that: the radicle length of the control group at day 8 is 34.87 mm, the longest length of the shell treatment group is 1:30 (45.09 mm), and the shortest length is stock solution (37.56 mm); the longest skin treatment group was 1:10 (40.93 mm) and the shortest was 1:100 (22.19 mm); the longest branch treatment group is 1:50 (47.41 mm), and the shortest branch treatment group is 1:30 (28.24 mm); the longest leaf treatment group was 1:100 (47.43 mm) and the shortest was stock solution (26.30 mm).

The results in table 1 and fig. 1 show that: the extract of the Grignard tannin extract has more obvious inhibition effect on the seed germination and radicle growth of the masson pine compared with extract of other litters, wherein the inhibition effect of the extract of the Grignard tannin extract is strongest, and the litters are selected to prepare the masson pine growth inhibitor, so that the extract of the Grignard tannin extract is easy to obtain and has no pollution to the environment.

The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.

Claims

1. An inhibitor for the growth of masson pine based on a leaching liquor of tannin extract litter is applied to inhibit the germination of masson pine seeds, and is characterized in that: the extract of the litters of the Grignard tannin extract is prepared by mixing a stock solution of the Grignard tannin extract with distilled water according to the proportion of 1:30 and 1:100, mixing a stock solution of the branches of the Grignard tannin extract with the distilled water according to the proportion of 1:10 and directly preparing a stock solution of the leaves of the Grignard tannin extract;

the preparation method of the leaching liquor stock solution of the castanopsis gracilis litter comprises the following steps: pulverizing the litters of the tannin extract, and then mixing according to the proportion of 1 g.5 mL^-1Adding distilled water into the castration of the tannin extract, soaking for a period of time, then filtering by using clean gauze, and finally carrying out suction filtration on the filtrate by using a vacuum pump to obtain the leaching liquor stock solution of the castration of the tannin extract.

2. The use of a extract of grahami extract based on an inhibitor of the growth of pinus massoniana of claim 1 for inhibiting the germination of pinus massoniana seeds, wherein: and crushing the castration of the tannin extract to 1-2 cm.

3. The use of a extract of grahami extract based on an inhibitor of the growth of pinus massoniana of claim 1 for inhibiting the germination of pinus massoniana seeds, wherein: soaking the litters of the tannin extract in distilled water for 48h at normal temperature.

4. The use of a extract of grahami extract based on an inhibitor of the growth of pinus massoniana of claim 1 for inhibiting the germination of pinus massoniana seeds, wherein: an inhibitor for the growth of masson pine based on the extract of litters of the Grignard tannin extract consists of the extract stock solution of the litters of the Grignard tannin extract.