CN110558338B - Inhibitor for growth of schima superba based on leaching liquor of castanopsis gracilis litter - Google Patents

Inhibitor for growth of schima superba based on leaching liquor of castanopsis gracilis litter Download PDF

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CN110558338B
CN110558338B CN201910997567.3A CN201910997567A CN110558338B CN 110558338 B CN110558338 B CN 110558338B CN 201910997567 A CN201910997567 A CN 201910997567A CN 110558338 B CN110558338 B CN 110558338B
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extract
inhibitor
stock solution
schima superba
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CN110558338A (en
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何中声
晋梦然
王哲
邢聪
朱静
王雪琳
李梦佳
刘金福
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Fujian Agriculture and Forestry University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]

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Abstract

The invention discloses an inhibitor for the growth of schima superba based on a leaching solution of a castanopsis gesii litter, belonging to the technical field of plant protection. The inhibitor is prepared by mixing a leaching liquor stock solution of a castration of a graham tannin extract with distilled water according to a certain proportion. The preparation method of the leaching liquor stock solution of the castanopsis gracilis litter comprises the following steps: crushing the castellated tannin extract, adding distilled water according to a certain proportion, soaking for a period of time, filtering by using clean gauze, and finally carrying out suction filtration on the filtrate by using a vacuum pump to obtain a castellated tannin extract liquid stock solution. The inhibitor for the growth of the schima superba is simple and easy to obtain raw materials, has no environmental pollution, and is simple, convenient and quick in preparation method. The inhibitor has a remarkable inhibiting effect on the germination of the schima superba seeds. The inhibitor of the invention has important guiding significance for protecting the rare tree species community from the chemical effect perspective to implement the protection of the Grignard tannin extract.

Description

Inhibitor for growth of schima superba based on leaching liquor of castanopsis gracilis litter
Technical Field
The invention belongs to the technical field of plant protection, and particularly relates to an inhibitor for the growth of schima superba based on a leaching solution of a castanopsis rabdosia litter.
Background
Extract of Grignard tannin (A)Castanopsis kawakamii) The plant is a evergreen tall tree with long growth cycle of castanopsis of Fagaceae, is only distributed in partial areas of south subtropical zone and middle subtropical zone south edge in China at present, and is an important target tree species for rare wild plant protection engineering in Fujian province. At present, the natural tannin extract forest establishment species of the Grignard tannin extract is in decline, the natural renewal of seedlings is poor, the population quantity of the first generation is small, the big trees are over-mature, and China fir is mainly mixed in the forest (China fir)Cunninghamia lanceolata) And (b) a wood lotusSchima superba) And masson pine (A)Pinus massoniana) And the like. In order to protect the rare species of the Girardinia extract, the previous people have studied from the aspects of population structure and distribution, soil space heterogeneity, forest gap microenvironment and the like. In addition, the litters of the natural forest of the tannin extract have large quantity and high tannin and total phenol content, and the allelopathy effect of the litters can influence the concomitant species of the litters. Therefore, the main associated species of the schima superba seeds are taken as tested seeds, the influence of the leaching liquor of the litters with different decomposition degrees and different concentrations on the germination and radicle growth of the schima superba seeds is researched, and the natural inhibitor for inhibiting the schima superba growth is sought. The experience is drawn from the perspective of allelopathy effect to the protection of the Grignard tannin extract, and the method has important guiding significance to the protection of other rare tree species communities.
Disclosure of Invention
The invention aims to provide an inhibitor for the growth of the schima superba based on a leaching solution of a castanopsis gracilis litter. The inhibitor for the growth of the schima superba has the advantages of easily available raw materials, simple and convenient preparation method, no environmental pollution and good inhibition effect on the growth of the schima superba.
In order to achieve the purpose, the invention adopts the following technical scheme:
a mangrove plant growth inhibitor based on a grahamia extract leaching solution is prepared by mixing a grahamia extract leaching solution stock solution and distilled water according to a certain proportion.
The preparation method of the leaching liquor stock solution of the castanopsis gracilis litter comprises the following steps: crushing the castellated tannin extract, adding distilled water according to a certain proportion, soaking for a period of time, filtering by using clean gauze, and finally carrying out suction filtration on the filtrate by using a vacuum pump to obtain a castellated tannin extract liquid stock solution.
Preferably, the castellated extract of the Girardinia gracilis is selected from the group consisting of branches, leaves, skins and shells of the Girardinia gracilis.
Preferably, the castration of the tannin extract is crushed to a size of 1-2 cm.
Preferably, 1 g.5 mL-1To the castration of tannin extract, distilled water was added.
Preferably, the litters of the tannin extract are soaked in distilled water for 48 hours at normal temperature.
The volume ratio of the mixed extract liquor of the castanopsis gracilis litter to the distilled water is as follows: 1:10, 1:30, 1:50, 1: 100.
Preferably, the inhibitor for the growth of the schima gracilis based on the kava extract leaching liquor consists of kava gracilis extract leaching liquor stock solution.
An inhibitor for the growth of the schima superba based on a kawasaki litter leaching solution is applied to inhibiting the germination of schima superba seeds.
The invention has the advantages that:
the invention provides an inhibitor for the growth of schima superba based on a leaching liquor of castanopsis gesii litter. The inhibitor is prepared by mixing the leaching liquor stock solution of the castration extract of the tannin extract with distilled water, and has the advantages of simple and easily obtained raw materials, no environmental pollution and simple and rapid preparation method. The inhibitor has a remarkable inhibiting effect on the germination of the schima superba seeds. The inhibitor of the invention has important guiding significance for protecting the rare tree species community from the chemical effect perspective to implement the protection of the Grignard tannin extract.
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FIG. 1: influence of different concentrations of the extract of the castration extract of the grist tannin extract on the growth of the radicle of the seed of the schima superba. A: a Grignard tannin extract; b: a Grignard tannin extract; c; a Grignard tannin extract; d: the extract of the leaf of the Kalanchoe extract.
Detailed Description
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the following examples are only examples of the present invention and do not represent the scope of the present invention defined by the claims.
Example 1
(1) Selecting materials:
collecting different types of litters in a fixed sample plot in a Fujian Sanming tannin extract protection area in 2018, wherein the litters are divided into 4 types of branches, leaves, skins and shells, and each type of litter is 4 Kg. Selecting the second generation of fine variety of the schima superba in the Longyan Zhanghei forest farm from the recipient plant schima superba seeds, and totally 1 Kg.
(2) Preparation of leach liquors
Pulverizing collected tannin extract branches, leaves, peels and shell litter to 1-2cm, and making into powder of 1 g.5 mL-1Adding a proper amount of distilled water into 1 g of dry matter according to a proportion, soaking for 48 hours at normal temperature, filtering by using clean gauze, and finally carrying out vacuum filtration on the filtrate by using a vacuum pump to obtain an extraction stock solution, mixing the extraction stock solution and the distilled water according to a volume ratio of 1:10, 1:30, 1:50 and 1:100 in consideration of leaching decomposition effect of litter in a natural state, preparing four kinds of leaching solutions with different concentrations respectively, and placing the four kinds of leaching solutions into a refrigerator at 4 ℃ for later use.
(3) Seed germination experiment
Selecting the schima superba seeds with uniform size and full particles, washing the schima superba seeds with distilled water for several times, and adding KMnO with the effective mass fraction of 0.5%4Sterilizing in the solution for 20 min, washing with distilled water repeatedly for 3 times, soaking in distilled water for 24 hr, and air drying to remove water. The germination bed consists of quantitative filter paper with the diameter of 15 cm and a culture dish, and the culture dish is wiped and disinfected by absolute alcohol before an experiment. Taking 5mL of leaching liquor with different concentrations and types, respectively adding into culture dishes paved with double-layer filter paper, uniformly placing 100 disinfected seeds in each culture dish, repeating for 3 times at each concentration, and adding distilled water with the same amount for treatment to serve as a control. Placing the culture dish in an artificial incubator with photoperiod of 14 h, 25 deg.C, dark period of 12h, 20 deg.C, and light intensity of 50nmol cm-2·s-1And the relative humidity is 75-80%. Equal amount (1-5 mL) of leaching liquor with corresponding concentration is supplemented every day to keep the filter paper moist.
(4) Determination of germination and morphological indices
Setting the seeds on the day 1 d, observing and recording the number of the germinated seeds every day, and finishing the germination experiment when no seeds germinate for 3 d continuously by taking the radicle exposed 1-2mm as a standard. And (3) selecting 20 seeds with basically consistent growth vigor from culture dishes with different concentrations in the full germination period of the seeds, measuring and recording the length of the radicle every two days, and finally calculating the germination rate, the germination vigor and the germination index.
Germination rate (%) = (number of seeds to germinate/total number of seeds to be tested) × 100%;
germination potential (%) = number of seeds normally germinated until the day when average germination number reaches the highest day/total number of test seeds × 100%;
germination index = ∑ (Gt/Dt）
Wherein:Dtthe number of days of germination is referred to,Gtfinger andDtcorresponding number of germinated seeds per day.
Results of the study
The effect of different concentrations and species of the leachate on the germination of schima superba seeds is shown in table 1. Table 1 the results show that: the germination rate of the control group of the schima superba seeds is 46.50%; the seed of the schima superba is extracted in the shell, branch, leaf and bark: after the stock solution and the treatment under the concentration of 1:10, 1:30, 1:50 and 1:100, the germination rate is 1:50 shells (45.00 percent) at the maximum, the stock solution and 1:50 peels (39.67 percent), 1:50 branches (52.87 percent) and 1:30 leaves (48.06 percent); the minimum germination rate is 1:100 shells (32.67%), 1:10 peels (31.67%), stock solution branches (46.14%), stock solution leaves (37.49%). The germination potential of the control group is 14.00%; the maximum germination potential of the treatment groups is 1:50 husks (10.84%), 1:50 peels (8.81%), 1:100 branches (12.00%), 1:50 leaves (14.50%); the smallest germinating potential was the stock solution shell (3.62%), the extract stock solution and 1:10 bark (4.51%), stock solution branches (8.00%), stock solution leaves (3.33%). The germination index of the control group was 56.12; the germination index of the treated group is maximum 1:50 shells (57.80), 1:50 peels (49.20), 1:100 branches (54.71) and 1:50 leaves (61.19); the minimum germination index is the stock solution shell (41.09), 1:10 bark (32.95), stock solution branch (41.58) and stock solution leaf (27.55).
TABLE 1 Effect of different concentrations and species of leach liquors on the Germination of Schima superba seeds
Tab1.Effects of different concentrations and kinds of extracts on germination of S. superba seeds
Figure DEST_PATH_IMAGE001
The effect of various concentrations of litter leachate on the radicle growth of schima superba seeds is shown in figure 1. The results in FIG. 1 show that: at the end of growth (growth to 8 d), the length of the radicle of the control group is 19.10 mm, the length of the radicle of the shell treatment group is 1:50 (27.08 mm) at the longest, and the length of the radicle of the shell treatment group is stock solution (15.16 mm) at the shortest; the length of radicle of the skin treatment group is 1:100 (23.04 mm) at most and 1:10 (17.52 mm) at least; the longest length of the radicle of the branch treatment group is 1:30 (27.66 mm), and the shortest length is the stock solution (19.76 mm); the longest length of radicle in the leaf treatment group is 1:100 (28.78 mm), and the shortest length is stock solution (12.42 mm).
The extract of the castration extract has more obvious inhibition effect on the germination of the seeds and the growth of radicles of the schima superba compared with other extract of the litters, wherein the extract of the castration extract has the strongest inhibition effect, and the litters are selected to prepare the schima superba growth inhibitor, so that the extract of the castration extract is easy to obtain and has no pollution to the environment.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.

Claims (4)

1. An inhibitor for the growth of the schima superba based on a kaki extract leaching solution is applied to inhibiting the germination of schima superba seeds, and is characterized in that: the extract of the castanopsis gracilis litter is specifically a castanopsis gracilis shell extract stock solution or a castanopsis gracilis shell extract stock solution and distilled water according to the ratio of 1: 10. mixing the extract stock solution of the tannin extract or the extract stock solution of the tannin extract and distilled water according to the proportion of 1:30 to 1:100, wherein the weight ratio of the extract stock solution of the tannin extract to the distilled water is 1: 10. mixing the extract liquid of the tannin extract branches in a ratio of 1:30, 1:50 and 1:100, directly preparing the extract liquid of the tannin extract branches, mixing the extract liquid of the tannin extract or the extract liquid of the tannin extract with distilled water according to a ratio of 1: mixing and blending according to a proportion of 10;
the preparation method of the leaching liquor stock solution of the castanopsis gracilis litter comprises the following steps: pulverizing the litters of the tannin extract, and then mixing according to the proportion of 1 g.5 mL-1Adding distilled water into the castration of the tannin extract, soaking for a period of time, then filtering by using clean gauze, and finally carrying out suction filtration on the filtrate by using a vacuum pump to obtain the leaching liquor stock solution of the castration of the tannin extract.
2. The use of an inhibitor of the growth of schima superba based on a kava extract of claim 1 for inhibiting the germination of schima superba seeds, wherein: and crushing the castration of the tannin extract to 1-2 cm.
3. The use of an inhibitor of the growth of schima superba based on a kava extract of claim 1 for inhibiting the germination of schima superba seeds, wherein: and soaking the castrated extract of the tannin extract in distilled water for 48 hours at normal temperature.
4. The use of an inhibitor of the growth of schima superba based on a kava extract of claim 1 for inhibiting the germination of schima superba seeds, wherein: the inhibitor for the growth of the schima gracilis based on the kaki extract leaching liquor consists of kaki gracilis extract leaching liquor stock solution.
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