CN113692803B

CN113692803B - Method for influencing germination of orychophragmus violaceus seeds by utilizing torch tree leaching liquor

Info

Publication number: CN113692803B
Application number: CN202111058067.7A
Authority: CN
Inventors: 付玲; 陈超
Original assignee: Tangshan Normal University
Current assignee: Tangshan Normal University
Priority date: 2021-09-09
Filing date: 2021-09-09
Publication date: 2022-12-27
Anticipated expiration: 2041-09-09
Also published as: CN113692803A

Abstract

The invention belongs to the technical field of plant leaching liquor, and particularly relates to a method for influencing germination of orychophragmus violaceus seeds by utilizing torch tree leaching liquor. Step 1: pretreatment of leaves, ears and roots of the torch; step 2: respectively preparing water extract for the pretreated leaves, ears and roots of the torch; and step 3: accelerating germination and preparing germination of the orychophragmus violaceus seeds; and 4, step 4: and (4) planting the February seeds prepared in the step (3), and adding the torch tree water extract prepared in the step (2) for germination and culture. The allelopathy of the torch tree on the orychophragmus violaceus is reflected in the influence of leaching solutions of different organs on the germination of orychophragmus violaceus seeds, the leaching solutions are obtained by treating the significant organs of the torch tree, the low-concentration torch leaf and root water leaching solutions promote the germination of the orychophragmus violaceus seeds, the high-concentration torch leaf and root water leaching solutions inhibit the germination of the orychophragmus violaceus seeds, and the cluster water leaching solutions inhibit the germination of the orychophragmus violaceus seeds and enhance the inhibition effect with the increase of the concentration.

Description

Method for influencing germination of orychophragmus violaceus seeds by utilizing torch tree leaching liquor

Technical Field

The invention belongs to the technical field of plant leaching liquor, and particularly relates to a method for influencing germination of orychophragmus violaceus seeds by utilizing torch tree leaching liquor.

Background

The torch tree, native Canada, was introduced in 1959 in China and popularized to the nation in 1974. The said plant is listed as afforestation and landscape tree species, mainly cultivated in the province in the north of yellow river basin, and used for afforestation of barren mountains and landscaping of saline-alkali barren lands. Due to the excellent adaptability, the torch tree is considered as an invasive plant by numerous scholars and may threaten native tree species and herbaceous plants.

The allergenicity is a field which is concerned and emphasized in recent years, and the research of the allergenicity of the torch tree by a plurality of scientific researchers mainly focuses on the influence of the water extract or alcohol extract of the torch tree on crops, vegetables, garden trees, herbaceous flowers and the like. The sensitivity of different crops to the water extract of the leaves of the torch tree is different, wherein the inhibition effect on the millet is stronger, the inhibition effect on the wheat and the mung bean is weaker, the toxicity effect on the wheat and the corn is good, and the toxicity effect is increased along with the increase of the concentration of the water extract and the prolonging of the treatment time. Research also shows that the water extract of three parts of the torch tree with different concentrations has allelopathy on corn and wheat, and the result shows that the water extract of the torch tree leaves has inhibition effect on the germination rate and activity index of the two seeds, and the allelopathy effect leaves are more than ears.

The soil decomposition products of the leaves of the torch trees have an inhibiting effect on the germination of the Shanghai green seeds and the growth of seedlings, and the higher the concentration of the leaching liquor is, the longer the time required for decomposition is, and the stronger the allelopathy is. The water extract of the whole leaves and the crushed fresh leaves of the torch tree has the effect of promoting the growth of the seedling height of the pakchoi, but has the effect of inhibiting the elongation of radicle, and the allelopathy strength is that the fresh leaves are more than the dry leaves. Researches find that different organs of the torch tree have different allelopathic effects on the same plant, and the water extract of each part of the torch tree has the allelopathic effect on the boswellia procumbens and has the effects of leaves, roots and ears. Certain allelochemicals exist in leaves and roots of the torch tree, and can inhibit the germination of the seeds of the Festuca arundinacea. The germination of the torch tree seeds is not influenced by the allelopathy effect of the torch tree, and the inhibition effect on the seed germination of the amorpha fruticosa is most obvious. The water extract of the whole leaves of the torch tree and the water extract of the soil under the forest have allelopathy on the germination of the seeds and the growth of the seedlings of Chinese toon, ailanthus altissima swingle, arborvitae and Chinese pine, and research results show that: the water extract of crushed fresh leaves of the torch tree can obviously inhibit the germination of the cedrela sinensis seeds; the under-forest soil water extract can remarkably inhibit the growth of the Chinese toon seedlings and has no remarkable growth promoting effect on the Chinese toon seedlings.

Along with the concept of urban greening, the garden land is higher and higher in plant status in the link of building a green city. The orychophragmus violaceus is cold-resistant and light-loving, has no strict requirement on soil and can grow in acid and alkaline barren lands. February has good ornamental value, and is a ground cover plant which is favored by northern regions for early spring flower viewing and green viewing in winter. Its excellent characteristics are popular with researchers. Many researchers have focused on the effect of orychophragmus violaceus on seed germination and seedling growth when orychophragmus violaceus is subjected to various stresses. The meadow bluegrass leaching liquor can inhibit the germination of the orychophragmus violaceus seeds, and the inhibition effect is that roots are stronger than leaves. Influence of sea salt stress on germination of orychophragmus violaceus seeds, and the result shows that the germination rate of the seeds is lower when the salt concentration is higher. The effect of the malachite leaching solution on the chlorophyll content and root growth of the orychophragmus violaceus seedlings is shown by the fact that high-concentration inhibition is promoted by low concentration.

The torch trees which are strong in adaptability, high in ornamental value and widely planted have the problems that vegetation under the trees is rare and biodiversity is low at present.

Disclosure of Invention

Aiming at the technical problems, the invention provides a method for influencing the germination of February orchids seeds by utilizing torch tree leaching liquor, which is used for better planting and protecting February orchids widely planted in China under a torch tree by comparing different influences of different parts of the torch tree leaching liquor on the germination of the February orchids seeds and the substance metabolism condition in the germination process.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a method for influencing the germination of February seeds by utilizing a torch tree leaching liquor comprises the following steps:

step 1: pretreatment of leaves, ears and roots of the torch;

step 2: respectively preparing water extract for the pretreated leaves, ears and roots of the torch;

and step 3: accelerating germination and preparing germination of the orychophragmus violaceus seeds;

and 4, step 4: and (3) planting the February seeds prepared in the step (3) in twelve culture dishes, and respectively adding the water extracts of different parts of the torch tree prepared in the step (2) for germination and culture.

Preferably, the pretreatment method of the leaves, ears and roots of the torch in the step 1 comprises the following steps:

she Yu treatment: selecting red leaves of the torch tree, washing the red leaves with distilled water, naturally drying the red leaves in the shade, crushing the red leaves by a crusher, and sealing and storing the crushed red leaves in a dry and cool place;

pre-treating the fruit cluster: washing fruit cluster with distilled water, naturally drying in the shade, pulverizing, and sealing in dry shade;

root pretreatment: washing the root with distilled water, air drying, cutting into 5 cm pieces, oven drying in oven, pulverizing, and sealing in dry shade.

Preferably, the raw materials of the leaves, the ears and the roots of the torch after the pretreatment in the step 1 are taken, distilled water is added according to the mass ratio of the raw materials to the water 1:4, the raw materials and the water are hermetically leached for 48 hours, the mixture is stirred for 4 times, and the mixture is filtered and centrifuged to obtain 0.25 g/mL ^-1 The torch leaf, the cluster and the root mother liquor are added with distilled water to be prepared into 0.025 g/mL ^-1 ，0.05g·mL ^-1 ，0.1g·mL ^-1 ，0.2g·mL ^-1 And (4) placing the water extract with four concentrations in an environment with the temperature of 4 ℃ for sealed storage for later use. Preferably, in the step 3, before sowing, the orychophragmus violaceus seeds are disinfected by 0.3% copper sulfate for 15min, then washed by distilled water for three times, and then dried in the shade for germination acceleration and germination for later use.

Preferably, in the

step

4, 100 orychophragmus violaceus seeds are placed in each culture dish, and 0.025 g/mL of torch leaves, ears and roots are added into the three culture dishes for the same treatment ^-1 ，0.05g·mL ^-1 ，0.1g·mL ^-1 ，0.2g·mL ^-1 2mL of the treatment solution, placing the culture dish in an artificial climate box for culture at 23 ℃, and supplementing the treatment solution with corresponding concentration for 1-2d to keep the filter paper moist.

Preferably, in the step 4, the growth index of the germination of the orychophragmus violaceus seeds is observed and measured every 24 hours, the length of the radicle exceeding the seeds is taken as a germination mark, the germination number of the tested plant is counted, and the germination rate, the germination index, the vigor index and the germination vigor are calculated.

Preferably, the determination of the physiological indicators of orychophragmus violaceus at 2d, 4d, 6d and 8d after germination comprises the following steps: proline content, reducing sugar content, soluble protein content, amylase activity.

Compared with the prior art, the invention has the beneficial effects that:

(1) The root system of the torch tree is shallow, the sprouting is fast, the part of the root is cut off and used for preparing the treatment liquid, the influence of the torch tree on the plant community can be relieved, all materials are ground before the leaching liquid is prepared, the leaching speed is accelerated, and the leaching effect is improved.

(2) The allelopathy of the torch tree on the February is reflected in the influence of the leaching liquor of different organs on the February seed germination.

(3) Fully combines the distribution characteristics and the growth habits of the plant community, and explores the allelopathy among the plants of different levels of the plant community.

(4) After the torch tree is crushed into powder, the powder can be stored for a long time, the powder can be stored at one time along with leaching, and the leaching liquor can be extracted only by water leaching.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.

In the drawings:

FIG. 1 is a flow diagram of a method of the present invention for affecting the germination of February seeds using a Rhus typhina leach solution;

FIG. 2 is a graph showing the effect of torch tree leaf water extract on the germination rate of February seeds;

FIG. 3 is a graph showing the effect of a water extract of ears of a torch tree on the germination rate of February's seeds;

FIG. 4 is a graph showing the effect of water extract of torch tree root on germination rate of February seeds;

FIG. 5 is a graph showing the effect of the leaching solution of leaves, ears and roots of torch on the germination potential of February seeds;

FIG. 6 is a graph showing the effect of the leach liquor of leaves, ears and roots of torch on the vitality index of February seeds;

FIG. 7 is a graph showing the effect of the leaching solution of leaves, ears and roots of torch on the germination index of February seeds;

FIG. 8 is a graph showing the effect of torch tree leaf aqueous extract on February proline content;

FIG. 9 is a graph of the effect of aqueous extract of ears of torch trees on the proline content of February seeds;

FIG. 10 is a graph of the effect of rhus typhina root water extract on February proline content;

FIG. 11 is a graph of the effect of torch leaf water extract on soluble protein content of February seeds; FIG. 12 is a graph of the effect of torch tree ear water extract on soluble protein content of February seeds;

FIG. 13 is a graph of the effect of rhus typhina root water extract on soluble protein content of February seeds;

FIG. 14 is a graph showing the effect of water extract of torch leaves on the activity of soluble amylase in germination rate of February seeds;

FIG. 15 is a graph showing the effect of aqueous extract of ears of torch trees on the activity of soluble amylase in February seeds;

FIG. 16 is a graph of the effect of aqueous extract of rhus typhina roots on the activity of soluble amylase from February seeds;

FIG. 17 is a graph of the effect of a water extract of flare leaves on the reducing sugar content of February seeds;

FIG. 18 is a graph of the effect of aqueous extract of ears of torch trees on the content of reducing sugars in February seeds;

FIG. 19 is a graph of the effect of rhus typhina root water extract on the reducing sugar content of February seeds.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.

Example 1:

step 1: pretreatment of leaves, ears and roots of the torch;

she Yu treatment: selecting healthy and disease-free red leaves, washing the red leaves with distilled water, naturally drying the red leaves in the shade, crushing the red leaves by a crusher, and sealing and storing the crushed red leaves in dry and cool places;

pre-treating the fruit cluster: cleaning healthy and disease-free fruit clusters with distilled water, naturally drying in the shade, pulverizing with a pulverizer, drying in the shade, and sealing;

root pretreatment: selecting root with uniform thickness and no putrefaction, washing with distilled water, air drying, cutting into 5 cm segments, air drying, oven drying, pulverizing, and sealing in dry and cool place.

Step 2: taking the raw materials of the leaves, the ears and the roots of the torch after the pretreatment in the step 1, adding distilled water according to the mass ratio of the raw materials to the water 1:4, leaching in a sealing way for 48 hours, stirring for 4 times, filtering and centrifuging to obtain 0.25 g/mL ^-1 The mother liquor of (2) was mixed with distilled water to prepare a mixture of 0.025 g/mL ^-1 ，0.05g·mL ^-1 ，0.1g·mL ^-1 ，0.2g·mL ^-1 Water extract of four concentrations;

wherein the leaf is 0.025 g/mL ^-1 The concentration of the leaching liquor is marked as A1,0.05 g.mL ^-1 The concentration of the leaching liquor is marked as A2,0.1 g.mL ^-1 The concentration of the leaching liquor is marked as A3,0.1 g.mL ^-1 The concentration mark of the leaching liquor is A4;

0.025 g/mL of the fruit cluster ^-1 The concentration of the leaching liquor is marked as B1,0.05 g.mL ^-1 The concentration of the leaching liquor is marked as B2,0.1 g.mL ^-1 The concentration of the leaching liquor is marked as B3,0.1 g.mL ^-1 The concentration mark of the leaching liquor is B4;

root of 0.025 g/mL ^-1 The concentration of the leaching liquor is marked as C1,0.05 g.mL ^-1 The concentration of the leaching liquor is marked as C2,0.1 g.mL ^-1 The concentration of the leaching liquor is marked as C3,0.1 g.mL ^-1 The concentration mark of the leaching liquor is C4; then placing the leaching liquor with different concentrations in an environment of 4 ℃ for sealed storage for later use.

And step 3: selecting full Orchium victoriae seeds with consistent size, sterilizing with 0.3% copper sulfate for 15min before sowing, rinsing with distilled water for three times, drying in shade, and performing germination acceleration and germination for later use.

And 4, step 4: in the

step

4, 100 February seeds treated in the step 3 are placed in each culture dish, each treatment is repeated for three times, and 0.025 g/mL of the torch leaves, the ears and the roots in the step 3 are added respectively ^-1 ，0.05g·mL ^-1 ，0.1g·mL ^-1 ，0.2g·mL ^-1 2mL of the treatment solution, placing the culture dish in an artificial climate box for culture at 23 ℃, and supplementing the treatment solution with corresponding concentration for 1-2d to keep the filter paper moist.

Example 2:

unlike the examples, distilled water, i.e., the concentration of the leachate was 0, was used in this example instead of the leachate as a control experiment.

Table 1 below shows the experimental treatment protocol for different concentrations of the leaching solution of leaves, ears and roots of torch in examples 1 and 2.

Table 1 test treatment protocol

Observing and measuring the February orchid seeds germinated in the examples 1 and 2, firstly observing and measuring the growth index of the February orchid seed germination every 24 hours, wherein the growth index is measured by taking the length of an embryonic root exceeding the seeds as a germination mark, counting the germination number of a tested plant, calculating the germination rate, the germination index, the vigor index and the germination vigor, and measuring the length of the embryonic root by randomly taking 5 seedlings from each culture dish by using a ruler on the 9 th day;

and measuring the 2d, 4d, 6d and 8d February physiological indexes after germination, wherein the measurement of the physiological indexes comprises the following steps: the method comprises the steps of determining the proline content by a ninhydrin colorimetric method, determining the reducing sugar content by a DNS colorimetric method, determining the soluble protein content by a Coomassie brilliant blue G-250 dyeing method, and determining the amylase activity by a3,5-dinitrosalicylic acid method.

And (3) analyzing growth index results:

the analysis of variance table 1 shows that the treatment of the activity index cluster group is obviously lower than CK; leaf group treatment A1 was significantly higher than CK, others were significantly lower than CK; the root group treatment was significantly higher than CK except C4. The treatment of the germination potential cluster group is obviously lower than CK; leaf group treatment was significantly lower than CK except A1; the root group treatments C1, C2 were significantly higher than CK, and C3, C4 were significantly lower than CK. The germination index cluster group treatment is obviously lower than CK; leaf group treatment was significantly lower than CK except A1; the root group treatments C1, C2 were significantly higher than CK, and C3, C4 were significantly lower than CK.

TABLE 1 comparison of difference significance of water extract of torch tree to germination of February seeds

(1) Influence of torch leaf, cluster and root water extract on germination rate of orychophragmus violaceus seeds

As can be seen from fig. 2, fig. 3 and fig. 4, the root and leaf water leaching solution of the torch tree has a low concentration promoting high concentration inhibition phenomenon on the germination of the orychophragmus violaceus seeds, and affects the effect: the root is larger than the leaf, the inhibition effect of the cluster water extract is most obvious, the cluster water extract is increased along with the increase of the concentration, the highest concentration of the three water extracts inhibits the germination of the orychophragmus violaceus seeds, the inhibition of the cluster water extract is the most serious, and the germination rate of the orychophragmus violaceus is less than 10%.

(2) Influence of torch leaf, cluster and root water extract on germination potential, germination index and vitality index of Orchidaceae plant seeds

As can be seen from fig. 5, 6 and 7, the germination vigor, germination index and vitality index of orychophragmus violaceus seeds, the water extract from roots and leaves at low concentration promoted or increased, the water extract from roots and leaves at high concentration inhibited or decreased, and the water extract from ears inhibited or decreased. And the germination vigor, the germination index and the vitality index of the orychophragmus violaceus are all reduced along with the increase of the concentration of the water extract. Wherein, the torch tree root water extract has the largest influence on the activity index, the C1 treatment is almost 7 times higher than the CK, and the A4 and B4 treatment activity indexes are almost 0.

Therefore, the ears of the torch trees have the greatest negative influence on the germination and growth of the February orchids, the high concentration of the leaves and the roots also has the negative influence on the February orchids, and the low concentration shows the promotion effect.

Analyzing the result of the physiological index:

(1) Influence of torch tree water extract on content of free proline in germination process of orychophragmus violaceus seeds

Analysis of variance table 2 shows that the control group was significantly lower on day two than the A2, A4 and C4 treatments, and was not significantly different from the other treatments; the control was significantly lower on day four than the A2, B3, B4, C3 and C4 treatments, with no significant difference from each of the other treatments; the control at day six was significantly lower than treatment with B1, B2 and B3, and was not significantly different from each of the other treatments; CK differed significantly from A4, B3, B4, C3, and C4 and was significantly lower than treatment group on day eight and not significantly different from each of the other treatments.

TABLE 2 influence of water extract of torch tree on proline content during germination of February seeds

As can be seen from fig. 8, 9 and 10, the content of orychophragmus violaceus proline treated by the water extract of the torch tree is decreased overall, but is higher than that of the control group, except that A4 is increased after reaching the lowest point on the sixth day; the change of the content of the orychophragmus violaceus proline treated by the cluster water extract is irregular, the difference is large, but the content of the proline at four concentrations treated by the cluster is higher than that of a control group, the proline at the four concentrations is sharply reduced after the proline at the four concentrations is at the highest point in the sixth day, and the proline at the four concentrations is increased after the proline at the four concentrations is at the lowest point in the sixth day; the proline content of the root water extract treatment group is generally in a descending trend, C3 and C4 rise after reaching the lowest point on the sixth day, and C1 and C2 rise first and then fall.

The data show that the February orchis seeds treated by the torch tree water leaching solution are stressed, the February orchis has adaptability to an adverse environment when being stressed, the torch tree water leaching solution may not have persistence on the stress of the February orchis, the stress degree is not aggravated, and the February orchis can adapt to the growth environment of the low-concentration torch tree water leaching solution.

(2) Influence of torch tree water extract on content of soluble protein in germination process of orychophragmus violaceus seeds

Analysis of variance table 3 shows that the control was significantly higher on day two than the B2, B4 and C4 treatments, with no significant difference from the other treatments; the control on the fourth day is significantly higher than the B1 treatment, and has no significant difference with other treatment groups; the control is obviously higher than the C3 and C4 difference treatment on the sixth day, and has no obvious difference with other treatments; the day eight control was significantly higher than the A4, B3 and B4 treatments and did not differ significantly from the other treatment groups.

Table 3 significant comparison of differences in soluble protein content of orychophragmus violaceus seeds under treatment with water extract from torch trees

Treatment of	2d th	4d th	6d th		8d th
						CK	8.05±0.16d	7.86±0.25abc	8.30±0.43a	8.18±0.46ab
A1	8.59±0.49cd	7.54±0.27abcd	7.68±0.19ab	8.15±0.64ab
					A2	8.51±0.74bcd	7.98±0.24abc	8.14±0.30a	7.22±0.12bc
A3	8.45±0.10bcd	7.81±0.06abc	8.12±0.28a	6.77±0.08c
					A4	8.08±0.13abcd	8.33±0.08ab	8.18±0.17a	4.32±0.82d
B1	8.14±0.15abcd	7.45±0.21abcd	8.00±0.10a	8.57±0.21a
					B2	6.21±0.37abc	6.27±1.02d	7.31±0.25ab	6.88±0.25bc
B3	6.94±0.16abc	7.18±0.24bcd	7.77±0.30ab	6.57±0.34c
					B4	4.75±1.13abc	6.79±0.23cd	7.29±0.23ab	4.97±0.33d
C1	6.88±0.92abc	7.72±0.06abc	7.04±0.04abc	8.70±0.24a
					C2	8.08±0.25abc	7.74±0.10abc	8.28±0.95a	7.84±0.70abc
C3	8.18±0.27ab	7.18±0.83bcd	6.52±0.83bc	7.08±0.18bc
					C4	6.2±1.11da	8.84±0.36a	5.81±0.42c	6.83±0.07bc

As can be seen from fig. 11, fig. 12 and fig. 13, the trend of the leaf water leaching solution changes and the content of the leaf water leaching solution is close to that of CK in 2-6 days, and the protein content decreases more with the increase of the concentration of the leaching solution after the sixth day; the protein content of the cluster water extract treatment group is generally lower than CK and shows a trend of ascending first and then descending, the protein content is reduced along with the increase of the concentration, and the protein content of the B1 group is higher than CK in the eighth day; the root water extract treatment groups have irregular changes, the contents of C1 and C3 are lower than CK, C3 is lower than C1, the content of C2 is close to trend change and CK, the C4 change amplitude is large, and the fluctuation is severe.

(3) Influence of torch tree water extract on amylase activity in germination process of orychophragmus violaceus seeds

The variance result analysis table 4 shows that the difference between the control and the C1 and C2 treatment on the next day is not significant, the difference between the control and other treatments is significant, and the control is significantly higher than other treatments; the control and C2 are not significantly different on the fourth day, C1 and C4 are significantly higher than the control, and the treatment except C1, C2 and C4 is significantly lower than the control; on the sixth day, C1 and C2 are significantly higher than the control, and other treatments are significantly lower than the control; the control on the eighth day has no significant difference with A2 and B2, A3, C1 and C2 are obviously higher than the control, and other treatments are all obviously lower than the control.

TABLE 4 significant comparison of differences in the activity of water extract of torch tree on soluble amylase from February seeds

As seen in fig. 14, 15 and 16, the root treatment groups C1, C2 treatment were higher than CK, and C3, C4 treatment were significantly lower than control; the ear treatment groups were all lower than the control, and B4 declined sharply after the sixth day; six days before the leaf treatment group were all below CK, and on the eighth day A3 treatment rose dramatically above CK.

The activity of the amylase of the seed treated by the three leaching solutions is generally in an increasing trend, and the activities are all reduced along with the increase of the concentration of the leaching solutions. The leaching liquor of the three parts of the torch tree has the inhibiting effect on the amylase activity, and the low concentration of the roots enhances the amylase activity.

(4) Influence of torch tree water extract on reducing sugar content in germination process of orychophragmus violaceus seeds

Analysis of variance table 5 shows that the control group did not significantly differ from the A1, A2, A3, C1, C3 and C4 treatments the next day, but significantly higher than the other treatments; day four controls were not significantly different from C2, C3 and C4 treatments, but significantly higher than the other treatments; the sixth day control was not significantly different from the A1, A2, B1, C2, C3, and C4 treatments, but significantly higher than the other treatment groups; on day eight CK was not significantly different from A1, A2, A3 and C2, significantly different from C4, B1, B2, B3, B4, C3 and C4, and significantly higher than each treatment.

TABLE 5 comparison of difference significance of water extract of torch tree to reducing sugar content of February seed

As can be seen from fig. 17, fig. 18 and fig. 19, the reducing sugar content of each treatment group as a whole showed an increasing trend, in which A1 and A2 were higher than CK; c1, C2 and CK were equal or above control, while other treatments were significantly lower than CK; all treatments in group B were lower than CK. This conclusion is also consistent with the partial amylase activity results. The reducing sugar content of each treatment increased as a whole with the increase of the treatment time, but the difference between different treatment groups and CK was significantly different. The reducing sugar content of the ear treatment group was always at a level significantly lower than CK; the leaf treatment groups except A4 have small differences with CK in other treatments; the reducing sugar content of the root treated group differed significantly from CK both at day 4 and day 8.

In conclusion, the results show that the low-concentration torch leaf and root water extract can promote seed germination, the promotion effect is that the root is larger than the leaf, the high-concentration torch leaf and root water extract can inhibit seed germination, the four concentrations of the ear water extract can inhibit seed germination, and the inhibition effect is enhanced along with the increase of the concentrations. Embodies that the torch tree has dual influence on the germination of the February seeds.

The stress resistance and adaptability of the February are reflected by the fact that the proline content is obviously increased under the treatment of the torch tree leaching liquor and is firstly reduced and then increased; the protein content change is not regular but the total protein content of the treatment group is lower than that of the control group; the activity of amylase and the content trend change of reducing sugar are obvious, except for the treatment of low-concentration root and leaf water extract, the other treated data are lower than those of a control group, and the duality of the influence of the torch tree root and leaf water extract on the germination of the February orchids seeds is proved again.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A method for influencing germination of February seeds by utilizing a torch tree leaching solution is characterized by comprising the following steps: the method comprises the following steps:

step 1: pretreatment of leaves, ears and roots of the torch;

and 4, step 4: planting the February seeds prepared in the step 3 in twelve culture dishes, and respectively adding the water extracts of different parts of the torch tree prepared in the step 2 for germination culture;

the pretreatment method of the leaves, the ears and the roots of the torch in the step 1 comprises the following steps:

pre-treating the fruit clusters: washing fruit cluster with distilled water, naturally drying in the shade, pulverizing, and sealing in dry shade;

root pretreatment: washing the root with distilled water, air drying, cutting into 5 cm segments, oven drying in oven, pulverizing, and sealing in dry shade;

taking the raw materials of the leaves, the ears and the roots of the torch after the pretreatment in the step 1, adding distilled water according to the mass ratio of the raw materials to the water 1:4, leaching in a sealing way for 48 hours, stirring for 4 times, filtering and centrifuging to obtain 0.25 g/mL ^-1 The torch tree leaves, the fruit ears and the root mother solution are added with distilled water to be prepared into 0.025 g/mL ^-1 ，0.05g·mL ^-1 ，0.1g·mL ^-1 ，0.2g·mL ^-1 Placing the water extract with four concentrations in an environment with the temperature of 4 ℃ for sealed storage for later use;

in the step 3, the orychophragmus violaceus seeds are disinfected by 0.3 percent of copper sulfate for 15min before sowing, then are washed for three times by distilled water, and then are dried in the shade for accelerating germination and sprouting for later use;

in the step 4, 100 February seeds are placed in each culture dish, and 0.025 g/mL of torch leaves, fruit ears and roots are added respectively ^-1 ，0.05g·mL ^-1 ，0.1g·mL ^-1 ，0.2g·mL ^-1 2mL of treatment solution, placing the culture dish in an artificial climate box for culture at 23 ℃, and supplementing the treatment solution with corresponding concentration for 1-2d to keep the filter paper moist;

in the step 4, observing and measuring the growth index of the February seed germination every 24 hours, counting the germination number of the tested plant and calculating the germination rate, the germination index, the vitality index and the germination potential by taking the length of the radicle exceeding the seed as a germination mark;

the method for determining the physiological indexes of the orychophragmus violaceus at 2d, 4d, 6d and 8d after germination comprises the following steps: proline content, reducing sugar content, soluble protein content, amylase activity.