CN110538113A

CN110538113A - Traditional Chinese medicine composition with moisturizing and hydrating effects and preparation method and application thereof

Info

Publication number: CN110538113A
Application number: CN201910927947.XA
Authority: CN
Inventors: 温艺慈; 胡根华; 吴知情; 何敬愉; 龚盛昭
Original assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Current assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Priority date: 2019-09-27
Filing date: 2019-09-27
Publication date: 2019-12-06

Abstract

the invention provides a traditional Chinese medicine composition with moisturizing and hydrating effects. The traditional Chinese medicine composition takes okra as a monarch drug, cactus as a ministerial drug, centella asiatica as an adjuvant drug and raspberry and tremella as a conductant drug; the traditional Chinese medicine components are matched with each other and synergized to achieve the effects of enhancing immunity and oxidation resistance of organisms, deeply replenishing water and keeping moisture for a long time, and the traditional Chinese medicine composition has the characteristics of pure nature, no stimulation, safety, no toxic or side effect and stable property.

Description

Traditional Chinese medicine composition with moisturizing and hydrating effects and preparation method and application thereof

Technical Field

The invention belongs to the field of cosmetics, and relates to a traditional Chinese medicine composition with moisturizing and hydrating effects, and a preparation method and application thereof.

Background

The stratum corneum of the epidermis of the skin is an important barrier for maintaining the health and stability of the skin, the water content of the healthy stratum corneum of the skin is kept above 10%, and the stratum corneum is directly dry and rough when the stratum corneum lacks water, and various skin problems such as thickening, desquamation, chapping, inflammation, pruritus, fine lines and the like are caused along with the skin. However, the dry and cold climate in autumn and winter is one of the important factors causing skin dryness, and besides, the influence of the working environment on the skin is not negligible, for example, modern urban office workers generally work in air-conditioned rooms for a long time and are easy to suffer from 'air-conditioning dessicator'. Therefore, dry and rough skin becomes a big skin problem which generally troubles people's life. According to statistics of an England latest product database, in Asian skin care products in recent years, cosmetics declared as moisturizing and moisturizing are ranked first, and it is seen that the first skin requirement of consumers is still moisturizing and moisturizing.

Moisturizers in cosmetics are classified into synthetic moisturizers and natural moisturizers according to their source. Polyols are the most widely used traditional synthetic moisturizers in cosmetics, such as glycerin, propylene glycol, sorbitol and the like, and these moisturizers can absorb moisture from the surrounding environment to keep the skin with a certain amount of moisture, but have good moisturizing effect only under the condition of high relative humidity, and can absorb moisture from the skin to make the skin drier in cold and dry environments with low relative humidity. In addition, the synthetic moisturizer can cause adverse reactions such as allergy, irritation and the like to the skin to a certain extent. The natural humectant is a raw material which is extracted from natural materials, has a good moisturizing effect and contains other substances with nutritive value to the skin, is popular with consumers, and has the characteristics of extraction from natural materials, easier skin absorption, reduction of skin irritation and the like, and is more popular with the environment-friendly concept of people's worried green and nature returning than a chemically synthesized humectant.

CN108938929A discloses a traditional Chinese medicine composition with moisturizing and anti-aging effects, a preparation method and an application thereof, wherein the traditional Chinese medicine composition is prepared from the following raw materials in percentage by mass: 15-40% of rose, 10-30% of dendrobium officinale, 10-25% of echinacea purpurea, 10-25% of aloe, 5-15% of tremella and 5-15% of seaweed.

CN103239383A discloses a traditional Chinese medicine composition for whitening and moisturizing, which is composed of the following raw materials in parts by weight: 5-10 parts of Chinese herbaceous peony, 8-15 parts of lotus, 3-10 parts of ganoderma sinensis, 4-15 parts of almond, 2-7 parts of motherwort, 3-12 parts of centella, 5-16 parts of silk, 7-18 parts of green bamboo, 2-6 parts of olive leaf, 5-15 parts of seaweed, 2-5 parts of oat, 3-9 parts of cactus, 1-5 parts of pearl, 3-12 parts of angelica, 7-15 parts of pawpaw, 4-16 parts of sophora flavescens and 5-10 parts of sanguisorba.

Therefore, the development of a traditional Chinese medicine composition which can deeply replenish water, can keep moisture for a long time and has strong oxidation resistance is the focus of research in the field of cosmetics at present.

disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide the traditional Chinese medicine composition with the moisturizing and hydrating effects and the preparation method and application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

In a first aspect, the invention provides a traditional Chinese medicine composition with moisturizing and hydrating effects, which is prepared from raw materials including okra, cactus, centella asiatica, raspberry and tremella.

The nature and the taste and the efficacy of the traditional Chinese medicines are as follows:

Okra: is cold in nature and bitter in taste, and has effects of nourishing yin, promoting fluid production, clearing heat, promoting diuresis, and enhancing immunity. The components such as polysaccharides and flavonoids have moisturizing and antioxidant effects.

Cactus: is cold in nature, light and astringent in flavor, and has the effects of invigorating spleen, benefiting stomach, clearing away heat and toxic materials, promoting blood circulation and activating qi. The cactus polysaccharide which is the main component is a good natural humectant.

Centella asiatica: cold in nature, bitter and pungent in flavor, clear heat and promote diuresis, detoxify and anti-inflammatory. The asiaticoside and asiatic acid contained in the product have anti-allergy, repairing and antioxidant effects.

Raspberry: warm in nature, sweet and sour in taste, nourish yin, tonify kidney, nourish liver and improve vision. The components such as polysaccharides, flavonoids, minerals and the like contained in the skin moisturizing cream can improve the skin moisturizing function and nourish and moisten the skin.

White fungus: mild in nature, sweet and bland in taste, nourishing yin, promoting the production of body fluid, clearing heat and moistening lung. The tremella polysaccharide has the effect of preventing skin moisture loss, and helps to keep skin moist and full.

The invention is based on the theory of traditional Chinese medicine, and each component of the traditional Chinese medicine composition is used by dispatching a prescription by applying the principle of monarch, minister, assistant and guide. The traditional Chinese medicine composition takes the okra as a monarch drug, researches show that the skin barrier damage is the main reason causing the skin dryness, the okra is an excellent immunomodulator, and the rich polysaccharide component of the okra can enhance the organism immunity, so that the traditional Chinese medicine composition has obvious promotion effects on improving the skin immunity and the skin barrier; the ministerial drug in the formula is cactus, and the polysaccharide and flavonoid components contained in the cactus not only have the effects of exogenously replenishing water and preserving moisture, but also can up-regulate the expression of human keratinocyte pangolin and assist the monarch drug in enhancing the effect of skin barrier function; centella asiatica is an adjuvant drug, and the skin physiology and the traditional Chinese medicine theory show that endogenous moisture of the skin can be transferred to the horny layer on the surface of the skin by inflammatory factors and toxic heat in vivo to cause the skin to be dry, and the effects of clearing heat and promoting diuresis, and resisting allergy and diminishing inflammation are taken to promote the endogenous moisture transfer, so that the skin is moisturized and full, and meanwhile, the toxicity of monarch and minister drugs can be inhibited, and the toxic and side effects of the traditional Chinese medicine composition are reduced; the raspberry and the tremella are messenger drugs, and the raspberry and the tremella are not only traditional good drugs for nourishing yin and moistening skin, but also good products for supplementing skin nutrition, and can be cooperatively matched to maintain skin nutrition balance, directly reach the skin water-deficient dry part, and simultaneously promote monarch drugs, ministerial drugs and adjuvant drugs to better exert the effects.

The traditional Chinese medicine composition disclosed by the invention is reasonable in formula component collocation, the traditional Chinese medicine components are matched with each other, the synergy is realized, the medicine performance is extremely exerted, the promotion effects of enhancing the immunity and oxidation resistance of the organism, improving the skin barrier, deeply replenishing water and keeping moisture for a long time are realized, meanwhile, the toxic and side effects of each component of the traditional Chinese medicine composition can be reduced, and the property is stable; the traditional Chinese medicine composition is applied to cosmetics, has good effects of moisturizing and moisturizing, follows the mainstream trend of cosmetics, meets the requirements of consumers, and solves various skin problems of rough skin, chapping skin, dryness and the like of the consumers.

preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 1-30% of okra, 1-30% of cactus, 1-30% of centella asiatica, 1-30% of raspberry and 1-30% of tremella.

In the present invention, the weight percentage of the okra in the raw materials for preparing the traditional Chinese medicine composition is 1-30%, for example, 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, 21%, 23%, 25%, 27%, 29%, 30%.

In the present invention, the weight percentage of cactus in the raw materials for preparing the traditional Chinese medicine composition is 1-30%, for example, 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, 21%, 23%, 25%, 27%, 29%, 30%.

In the present invention, the raw material of the traditional Chinese medicine composition comprises 1-30% by weight of centella asiatica, for example, 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, 21%, 23%, 25%, 27%, 29%, 30%.

in the present invention, the weight percentage of the raspberry in the raw materials for preparing the traditional Chinese medicine composition is 1-30%, for example, 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, 21%, 23%, 25%, 27%, 29%, 30%.

In the invention, the weight percentage of the tremella in the raw materials for preparing the traditional Chinese medicine composition is 1-30%, for example, 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 17%, 19%, 21%, 23%, 25%, 27%, 29%, 30%.

Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 10-30% of okra, 10-30% of cactus, 10-20% of centella, 10-20% of raspberry and 10-20% of tremella.

preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 30% of okra, 30% of cactus, 20% of centella, 10% of raspberry and 10% of tremella.

preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 25% of okra, 25% of cactus, 20% of centella asiatica, 15% of raspberry and 15% of tremella.

Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 20% of okra, 20% of cactus, 20% of centella, 20% of raspberry and 20% of tremella.

In a second aspect, the present invention provides a preparation method of the traditional Chinese medicine composition with moisturizing and hydrating effects according to the first aspect, the preparation method comprises the following steps:

(1) Pulverizing the components of the traditional Chinese medicine composition, sieving and uniformly mixing to obtain mixed powder;

(2) Adding a solvent into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution, and then concentrating, precipitating and drying the extracting solution to obtain a crude product;

(3) and (3) adding a solvent into the crude product obtained in the step (2) for dissolving, and filtering to obtain the traditional Chinese medicine composition.

preferably, the mesh number of the mixed powder in the step (1) is 100-200 meshes, for example, 100 meshes, 105 meshes, 110 meshes, 115 meshes, 120 meshes, 125 meshes, 130 meshes, 135 meshes, 140 meshes, 145 meshes, 150 meshes, 155 meshes, 160 meshes, 165 meshes, 170 meshes, 175 meshes, 180 meshes, 185 meshes, 190 meshes, 200 meshes can be provided.

Preferably, the mass ratio of the mixed powder to the solvent in the step (2) is 1 (2-20), and may be, for example, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, preferably 1 (10-20).

preferably, the solvent in step (2) comprises any one or a mixture of at least two of water, butanediol, propylene glycol or ethanol, preferably water or ethanol.

preferably, the volume ratio concentration of the ethanol is 1-10%; for example, it may be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%.

Preferably, the temperature of the extraction in step (2) is 45-85 deg.C, such as 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 75 deg.C, 80 deg.C, 85 deg.C.

Preferably, the number of times of extraction in step (2) is 2-5, for example, 2, 3, 4, 5, preferably 3.

preferably, the time of each extraction in the extraction of step (2) is 1-3h, and can be 1h, 1.2h, 1.4h, 1.6h, 1.8h, 2h, 2.2h, 2.4h, 2.6h, 2.8h and 3h, for example.

Preferably, the concentration in step (2) comprises the following specific steps: mixing the extractive solutions, filtering, and concentrating.

preferably, the concentration of step (2) is concentration under reduced pressure.

Preferably, the specific steps of the precipitation in the step (2) are as follows: adding solvent into the concentrated extract, performing fractional precipitation, centrifuging, and collecting.

Preferably, the solvent is ethanol.

Preferably, the rotational speed of the centrifugal collection is 3000-5000r/min, such as 3000r/min, 3200r/min, 3400r/min, 3600r/min, 3800r/min, 4000r/min, 4200r/min, 4400r/min, 4600r/min, 4800r/min and 5000 r/min.

Preferably, the drying of step (2) is vacuum drying;

Preferably, the drying temperature in step (2) is 50-70 deg.C, such as 50 deg.C, 52 deg.C, 54 deg.C, 56 deg.C, 58 deg.C, 60 deg.C, 62 deg.C, 64 deg.C, 66 deg.C, 68 deg.C, 70 deg.C, preferably 60 deg.C.

preferably, the solvent in step (3) comprises any one of water, butanediol or propylene glycol or a mixture of at least two of them, preferably water and/or butanediol.

preferably, the mass ratio of water to butanediol is 1 (0.25-2), and may be, for example, 1:0.25, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, preferably 1:1.

Preferably, the water is deionized water.

Preferably, the dissolving in step (3) comprises the following specific steps: and (3) adding water into the crude product obtained in the step (2) for dissolving, and then adding butanediol for redissolving.

Preferably, the preparation method comprises the following steps:

(1) pulverizing the components of the Chinese medicinal composition, sieving, and mixing to obtain 100-200 mesh mixed powder;

(2) Adding a solvent into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution, wherein the mass ratio of the mixed powder to the solvent is 1 (2-20), the extraction temperature is 45-85 ℃, the extraction frequency is 2-5 times, the extraction time is 1-3h each time, the extracting solutions obtained by each time of extraction are combined, filtered and concentrated, the solvent is added into the concentrated extracting solution, fractional precipitation and centrifugal collection are carried out, the rotational speed of the centrifugal collection is 3000 plus 5000r/min, and finally, vacuum drying is carried out at 50-70 ℃ to obtain a crude product;

(3) and (3) adding a solvent into the crude product obtained in the step (2), dissolving and filtering to obtain the traditional Chinese medicine composition.

In a third aspect, the invention provides an application of the traditional Chinese medicine composition with the moisturizing and hydrating effects in the first aspect in preparing cosmetics.

In a fourth aspect, the present invention provides a cosmetic comprising the traditional Chinese medicine composition with moisturizing and hydrating effects according to the first aspect.

Preferably, the moisture retention and water supplement effect traditional Chinese medicine composition is added in an amount of 0.05-20% of the total weight of the cosmetic, for example, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.3%, 0.5%, 0.7%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, preferably 0.1-10%.

preferably, the cosmetic comprises any one of a mask, a smoothing toner, a essence, a moisturizing cream, a toner, or a BB cream.

Compared with the prior art, the invention has the following beneficial effects:

(1) The traditional Chinese medicine composition disclosed by the invention takes okra as a monarch drug, cactus as a ministerial drug, centella asiatica as an adjuvant drug, raspberry and tremella as a conductant drug, and the traditional Chinese medicine components are matched with each other, so that the traditional Chinese medicine composition has a synergistic interaction effect, achieves the effects of enhancing the oxidation resistance of skin, deeply replenishing water and durably moisturizing, and has the characteristics of pure nature, no irritation, safety, no toxic or side effect and stable property.

(2) The traditional Chinese medicine composition disclosed by the invention has the effects of deeply replenishing water and durably moisturizing, and is specifically shown in that the moisturizing rate reaches over 35% after the traditional Chinese medicine composition is used for 4 hours, and the moisturizing rate reaches over 34% after the traditional Chinese medicine composition is used for 8 hours; the traditional Chinese medicine composition has strong antioxidant capacity, the half clearance rate IC50 of DPPH free radicals is between 10 and 21 mu g/mL, the ratio is equivalent to that of a high-efficiency antioxidant VC, and the antioxidant capacity of the traditional Chinese medicine composition is comparable to that of VC.

Drawings

Fig. 1 is a schematic diagram of test results of the moisturizing and hydrating essence prepared in application example 2 and application comparative examples 1 to 10 on the roughness of human skin.

Fig. 2 is a schematic diagram of test results of the moisturizing and hydrating essence prepared in application example 2 and application comparative examples 1 to 10 on the scale level of human skin.

Fig. 3 is a schematic diagram of trial results of the population of the moisturizing cream prepared in application example 3 and application comparative examples 11 to 20.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Example 1

The embodiment provides a traditional Chinese medicine composition with moisturizing and water replenishing effects, and the traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 30% of okra, 30% of cactus, 20% of centella, 10% of raspberry and 10% of tremella.

The preparation method comprises the following steps:

(1) Taking 30g of okra, 30g of cactus, 20g of centella, 10g of raspberry and 10g of tremella, crushing, sieving and uniformly mixing to obtain mixed powder of 100 meshes;

(2) Adding deionized water into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution, wherein the mass ratio of the mixed powder to a solvent is 1:10, the extraction temperature is 80 ℃, the extraction times are 3 times, each time is 1h, the extracting solutions are combined and filtered, the extracting solution is concentrated under reduced pressure until the mass ratio of the extracting solution to the total mass of the components in the step (1) is 1:1, adding 30% ethanol by volume fraction into the concentrated extracting solution for multistage alcohol precipitation, centrifugally collecting precipitates, wherein the rotational speed of the centrifugal collection is 3000r/min, and finally performing vacuum drying at 60 ℃ to obtain a crude product;

(3) and (3) adding deionized water into the crude product obtained in the step (2) to dissolve, then adding the same amount of butanediol to redissolve, and filtering to obtain the traditional Chinese medicine composition extract.

Example 2

The embodiment provides a traditional Chinese medicine composition with moisturizing and water replenishing effects, and the traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 25% of okra, 25% of cactus, 20% of centella asiatica, 15% of raspberry and 15% of tremella.

The preparation method comprises the following steps:

(1) Taking 25g of okra, 25g of cactus, 20g of centella asiatica, 15g of raspberry and 15g of tremella, crushing, sieving and uniformly mixing to obtain mixed powder of 150 meshes;

(2) Adding deionized water into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution, wherein the mass ratio of the mixed powder to a solvent is 1:15, the extraction temperature is 85 ℃, the extraction times are 3 times, each time is 2 hours, the extracting solutions are combined and filtered, the extracting solution is concentrated under reduced pressure until the mass ratio of the extracting solution to the total mass of the components in the step (1) is 1:1, ethanol with the volume fraction of 50% is added into the concentrated extracting solution for multistage alcohol precipitation, the precipitate is centrifugally collected, the rotational speed of the centrifugal collection is 5000r/min, and finally, the crude product is obtained by vacuum drying at the temperature of 60 ℃;

Example 3

The embodiment provides a traditional Chinese medicine composition with moisturizing and water replenishing effects, and the traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 20% of okra, 20% of cactus, 20% of centella, 20% of raspberry and 20% of tremella.

The preparation method comprises the following steps:

(1) Taking 25g of okra, 25g of cactus, 20g of centella asiatica, 15g of raspberry and 15g of tremella, crushing, sieving and uniformly mixing to obtain mixed powder of 200 meshes;

(2) adding deionized water into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution, wherein the mass ratio of the mixed powder to a solvent is 1:20, the extraction temperature is 85 ℃, the extraction times are 3 times, each time is 3 hours, the extracting solutions are combined and filtered, the extracting solution is concentrated under reduced pressure until the mass ratio of the extracting solution to the total mass of the components in the step (1) is 1:1, ethanol with the volume fraction of 70% is added into the concentrated extracting solution for multistage alcohol precipitation, the precipitate is centrifugally collected, the rotational speed of the centrifugal collection is 4000r/min, and finally, the crude product is obtained by vacuum drying at the temperature of 60 ℃;

Example 4

The preparation method comprises the following steps:

(1) Taking 30g of okra, 30g of cactus, 20g of centella, 10g of raspberry and 10g of tremella, crushing, sieving and uniformly mixing to obtain 120-mesh mixed powder;

(2) adding deionized water into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution, wherein the mass ratio of the mixed powder to a solvent is 1:2, the extraction temperature is 45 ℃, the extraction times are 5 times, each time is 1h, the extracting solutions are combined and filtered, the extracting solution is concentrated under reduced pressure until the mass ratio of the extracting solution to the total mass of the components in the step (1) is 1:1, adding 50% ethanol by volume fraction into the concentrated extracting solution for multistage alcohol precipitation, centrifugally collecting precipitates, wherein the rotational speed of the centrifugal collection is 3000r/min, and finally performing vacuum drying at 50 ℃ to obtain a crude product;

Example 5

The embodiment provides a traditional Chinese medicine composition with moisturizing and water replenishing effects, and the traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 10% of okra, 20% of cactus, 20% of centella, 20% of raspberry and 30% of tremella.

The preparation method comprises the following steps:

(1) taking 10g of okra, 20g of cactus, 20g of centella, 20g of raspberry and 30g of tremella, crushing, sieving and uniformly mixing to obtain 150-mesh mixed powder;

example 6

The embodiment provides a traditional Chinese medicine composition with moisturizing and water replenishing effects, and the traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 10% of okra, 10% of cactus, 30% of centella, 30% of raspberry and 20% of tremella.

The preparation method comprises the following steps:

(1) Taking 10g of okra, 10g of cactus, 30g of centella, 30g of raspberry and 20g of tremella, crushing, sieving and uniformly mixing to obtain 150-mesh mixed powder;

example 7

The embodiment provides a traditional Chinese medicine composition with moisturizing and water replenishing effects, and the traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 40% of okra, 40% of cactus, 10% of centella, 5% of raspberry and 5% of tremella.

The preparation method comprises the following steps:

(1) Taking 40g of okra, 40g of cactus, 10g of centella, 5g of raspberry and 5g of tremella, crushing, sieving and uniformly mixing to obtain 180-mesh mixed powder;

comparative example 1

The same as example 1, except that the raw materials for preparing the traditional Chinese medicine composition only contain 100g of okra, and do not contain other traditional Chinese medicine components, and other preparation methods are the same.

comparative example 2

the difference from the example 1 is that the raw materials for preparing the traditional Chinese medicine composition only contain 100g of cactus, and do not contain other traditional Chinese medicine components, and other preparation methods are the same.

comparative example 3

the same as example 1, except that the raw materials for preparing the Chinese medicinal composition only contain 100g of centella asiatica and no other Chinese medicinal components, and the other preparation methods are the same.

comparative example 4

The same as example 1, except that the raw materials for preparing the traditional Chinese medicine composition only contain 100g of raspberry, and do not contain other traditional Chinese medicine components, and other preparation methods are the same.

Comparative example 5

The difference from the example 1 is that the raw materials for preparing the traditional Chinese medicine composition only contain 100g of tremella and do not contain other traditional Chinese medicine components, and other preparation methods are the same.

Comparative example 6

The same as example 1, except that the raw materials for preparing the traditional Chinese medicine composition comprise 35g of okra, 35g of cactus and 30g of centella asiatica, and other preparation methods are the same.

comparative example 7

The same as example 1, except that the raw materials for preparing the traditional Chinese medicine composition comprise 40g of cactus, 30g of centella asiatica, 15g of raspberry and 15g of tremella, and other preparation methods are the same.

Comparative example 8

the same as example 1, except that the raw materials for preparing the traditional Chinese medicine composition comprise 40g of okra, 30g of centella asiatica, 15g of raspberry and 15g of tremella, and other preparation methods are the same.

comparative example 9

the difference is that the preparation raw materials of the traditional Chinese medicine composition comprise 35g of okra, 35g of cactus, 15g of raspberry and 15g of tremella.

Test example 1

Haemolysis test of erythrocytes

The erythrocyte hemolysis test is one of the alternatives of the rabbit eye irritation test (Draize test), and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the dissolution amount and denaturation degree of hemoglobin. The RBC test is used internationally for evaluating eye irritation studies on chemicals such as cosmetics and raw materials.

the Chinese medicinal compositions prepared in examples 1 to 7 and the Chinese medicinal extracts prepared in comparative examples 1 to 9 were subjected to a red blood cell hemolysis test in accordance with the RBC test method and grade standard of the European Alternatives Committee for the validation of methods (ECVAM). ECVAM. ECVAM DB-ALM INVITOX Protoco, Red blood cells test system INVITOX No.37 Ispra. Italy ECVAM,1994 ], ECVAM cosmetic products RBC experimental irritation grading criteria are shown in Table 1:

TABLE 1

the results of experiments on hemolysis of erythrocytes of the herbal compositions prepared in examples 1 to 7 and the herbal extracts prepared in comparative examples 1 to 9 are shown in Table 2, in which HD50 is the sample concentration at which 50% of erythrocytes are hemolyzed, DI is the protein denaturation index, and L/D is the ratio of HD50 to DI.

TABLE 2

According to the test results, the irritation of the traditional Chinese medicine composition prepared in the embodiments 1 to 7 is classified as no irritation, which indicates that the traditional Chinese medicine composition has the characteristics of mildness and no irritation; wherein, the sample concentration HD50 of the traditional Chinese medicine composition prepared in the examples 1-7 when 50% of erythrocytes are hemolyzed is more than 70096mg/L and is far greater than HD50 of the traditional Chinese medicine extract prepared in the comparative examples 1-9, and the protein denaturation index DI is less than 0.55% and is also obviously less than the traditional Chinese medicine extract prepared in the comparative examples 1-9, which indicates that the five traditional Chinese medicine compositions are mutually matched, and the toxic and side effects and the irritation of each traditional Chinese medicine can be obviously reduced after the synergy, and the traditional Chinese medicine composition has scientific and reasonable component collocation.

Test example 2

in vitro weighing method moisturizing performance test research

The principle of the in vitro weighing method for testing the moisture retention performance is as follows: according to the difference of moisture absorption and retention performances of different components, the acting force of different humectant molecules on water molecules is different, and the capacities of absorbing and retaining water are different. The adhesive tape is used for simulating biological materials such as cuticle and the like, the adhesive tape is coated with the humectant and placed for a period of time under the conditions of constant temperature and constant humidity, the mass of the sample placed for different periods of time is weighed, and the moisture retention rate of the sample can be calculated, so that the moisture retention performance of the sample can be evaluated.

The tests were conducted using the conventional high-performance humectant glycerin for cosmetics as a control sample, the Chinese medicinal composition prepared in the above examples 1 to 7 and the medicinal extracts prepared in the comparative examples 1 to 9. The test was as follows:

1. Experimental procedure

The glycerin, the traditional Chinese medicine composition prepared in examples 1 to 7 and the traditional Chinese medicine extract of comparative examples 1 to 9 are respectively weighed and 0.2000g is respectively and uniformly coated on a glass plate which is 5cm by 5cm and is stuck with the microporous ventilating adhesive tape, the glass plate is placed in a dryer with constant temperature and constant humidity, the mass of the glass plate after being placed for 4 hours and 8 hours is respectively weighed, and the moisture retention rate is calculated.

the moisture retention rate calculation formula is as follows:

moisture retention inhibition rate/% (M2-M0)/(M1-M0) × 100%

Wherein M0 is the glass plate mass/g, M1 is the glass plate mass/g after sample loading, and M2 is the glass plate mass/g after being placed in a dryer for several hours.

2. Results of the experiment

The results of the in vitro weighing method for determining the moisture retention rate of the traditional Chinese medicine composition prepared in examples 1 to 7, the traditional Chinese medicine extract prepared in comparative examples 1 to 9 and the glycerin serving as a control sample are shown in the following table 3:

TABLE 3

As can be seen from table 3, the moisture retention rates of glycerin and the Chinese medicinal compositions prepared in examples 1 to 4 were higher than 35% at 4 hours and higher than 34% at 8 hours, and the moisture retention rates were within 1% with time, and the moisture retention effect was stable; the traditional Chinese medicine composition prepared in the embodiments 1 to 4 has higher moisturizing rate than glycerin in 4 hours and 8 hours, which shows that the traditional Chinese medicine composition has superior moisturizing performance to the traditional high-efficiency moisturizing agent glycerin and has excellent moisturizing effect. Meanwhile, the experimental results show that the moisture retention rates of the traditional Chinese medicine extracts prepared in the comparative examples 1-9 for 4h and 8h are far lower than that of the traditional Chinese medicine composition, and the moisture retention effect is not as stable as that of the traditional Chinese medicine composition, which indicates that the components of the traditional Chinese medicine composition are mutually matched to play a synergistic effect so as to achieve the effects of deep water replenishing and lasting moisture retention.

Test example 3

Research on influence of human skin moisture content

the water content of the human skin stratum corneum is measured by adopting a capacitance method, the difference of dielectric constants of water and other substances is obvious, the measured capacitance value of the skin is different according to the difference of the water content of the skin stratum corneum, and the parameter can represent the water content of the skin.

The test was performed using the herbal composition prepared in examples 1-7 and the herbal extract prepared in comparative examples 1-9. The test was as follows:

3 experimental areas of 3 × 3cm2 size were respectively drawn on the inner sides of the left and right forearms of the testee, the left arm was used as a test area of the herbal composition extract, the herbal compositions prepared in examples 1 to 7 and the herbal extracts prepared in comparative examples 1 to 9 were respectively subjected to the test and uniformly spread on the corresponding test areas, the corresponding symmetric area of the right arm was used as a blank control, the moisture content of the skin before the sample was spread on the test area and the blank area of the skin moisture tester and after the sample was spread for 2 hours, and the evaluation criteria of table 4 were performed. Wherein, the change value of the skin moisture content in 2h is the skin moisture content in the test area after the sample is smeared for 2 h-the skin moisture content in the test area before smearing, and the specific test results are shown in table 5.

TABLE 4

TABLE 5

from the test results, the traditional Chinese medicine compositions prepared in the embodiments 1 to 7 of the present invention have a change value of more than 25% in water content for 2 hours, and have excellent moisture retention effect grade, which indicates that the traditional Chinese medicine composition of the present invention can effectively increase the moisture content of skin, and has excellent and lasting moisturizing effect; the change value of the water content of the traditional Chinese medicine extract prepared in the comparative examples 1-9 in 2h is obviously less than 25%, the grade of the moisturizing effect is good or general, and the traditional Chinese medicine extract is not the traditional Chinese medicine composition prepared in the examples 1-7, so that the traditional Chinese medicine composition can achieve the matching effect of monarch, minister, assistant and guide, has the synergistic effect, obviously enhances the moisturizing effect, and is reasonable in formula collocation of the traditional Chinese medicines.

test example 4

study of DPPH radical scavenging ability

The ability to scavenge DPPH free radicals reflects to some extent the antioxidant capacity of the material. The greater the free radical clearance rate, the stronger the antioxidant capacity and the stronger the anti-aging capacity. Therefore, the anti-aging effect of the traditional Chinese medicine composition can be judged by researching the capability of the traditional Chinese medicine composition for eliminating DPPH free radicals.

The tests were carried out using the Chinese medicinal compositions prepared in examples 1 to 7 and comparative examples 1 to 9. The test was as follows:

Adding 2mL of DPPH test solution with the mass concentration of 47.7mg/L and a certain amount of sample to be tested into a test tube, supplementing the total volume to 3mL by absolute ethyl alcohol, shaking up, reacting for 30min in a dark place, and measuring the absorbance at the wavelength of 517nm by using a 1cm cuvette and marking As; adding 2mL of absolute ethyl alcohol and a sample to be detected with a corresponding volume into the test tube, supplementing the total volume to 3mL by the absolute ethyl alcohol, and recording the measured absorbance as Ar; 2mL of DPPH test solution and 1mL of absolute ethanol were added to the tube, and the absorbance measured was recorded as A0.

The DPPH radical clearance (Y) is calculated as: y (%). 1- (As-Ar)/A0X 100%

The DPPH half-clearance (IC50, μ g/mL) of butanediol, VC standard solution, examples 1-7, and comparative examples 1-9 were determined in this order according to the procedure described above.

TABLE 2 measurement of DPPH clearance for Chinese medicinal composition

In the experiment, the antioxidant effect of the sample is evaluated by taking the DPPH half-clearance rate IC50 as an index, and the smaller the DPPH half-clearance rate IC50 value is, the stronger the clearance capability is, the better the antioxidant effect is. VC is a high-efficiency antioxidant accepted by the cosmetic industry, and the test results show that the DPPH half clearance rate IC50 of the traditional Chinese medicine composition prepared in the examples 1-7 is between 10 and 21 mu g/mL, and the traditional Chinese medicine composition prepared in the examples 1-4 is even close to IC50 of VC, so that the traditional Chinese medicine composition has good DPPH free radical scavenging capability, and the traditional Chinese medicine composition prepared in the invention has obvious effects of scavenging free radicals and resisting oxidation; the semi-clearance rate IC50 of DPPH of the traditional Chinese medicine extracts prepared in comparative examples 1-9 is far greater than that of IC50 of VC, and the clearance rate of DPPH free radicals is not ideal, which shows that the traditional Chinese medicine composition prepared by the invention has obvious synergistic effect on all components, has excellent clearance rate of DPPH free radicals, can effectively reduce active oxygen free radicals of skin, resists skin injury and has good antioxidant effect.

Application example 1

this application example provides a formula of moisturizing and hydrating smoothing toner containing the traditional Chinese medicine composition prepared in example 1, which is prepared from the components in the weight percentage shown in table 7 (i.e., the numbers in the table represent the weight percentage, such as 5 for 5%).

the preparation method of the moisturizing and hydrating smoothing toner comprises the following steps: adding butanediol, glycerol, EDTA disodium, D-panthenol, p-hydroxyacetophenone and the traditional Chinese medicine combined extract into deionized water, stirring until the materials are completely dissolved, and performing vacuum degassing treatment to obtain the moisturizing and hydrating smoothing toner.

TABLE 7

Application example 2

The application example provides a formula of the moisturizing essence containing the traditional Chinese medicine composition prepared in example 1, and the formula is prepared from the components with the weight percentage shown in table 8 (namely, the numbers in the table represent the weight percentage, for example, 5 represents 5%):

TABLE 8

The preparation method of the moisturizing essence comprises the following steps:

(1) heating the component A to 70 ℃ to completely dissolve the component A;

(2) Cooling the component A in the step (1) to 45 ℃, adding the phase B, and stirring for neutralization;

(3) and (3) after the neutralization in the step (2) is finished, adding the component C for dissolving, and uniformly stirring.

Application example 3

The application example provides a formula of the moisturizing and hydrating cream containing the traditional Chinese medicine composition prepared in the example 1, which is prepared from the components in percentage by weight shown in table 9 (namely, the numbers in the table represent the percentage by weight, for example, 5 represents 5%):

TABLE 9

The preparation process of the moisturizing and hydrating cream in the embodiment comprises the following steps:

(1) Heating the component A to 70 ℃ to completely dissolve the component A;

(2) heating the component B to 70 ℃ to completely dissolve the component B;

(3) adding the A into the B component while stirring, stirring at 70 deg.C for 20min, and homogenizing for 1 min;

(4) And (4) cooling the mixed components obtained in the step (3) to 45 ℃, adding the component C, and uniformly stirring.

Application of comparative examples 1 to 10

The moisturizing and hydrating essence of application comparative examples 1 to 10 is prepared from the components in the weight percentage shown in table 10 (i.e., the numbers in the table represent the weight percentage, for example, 5 represents 5%), and the preparation method of the application comparative examples 1 to 10 is the same as the preparation method of the moisturizing and hydrating essence of application example 2.

Watch 10

Comparative examples of application 11 to 20

The moisturizing and hydrating creams of application comparative examples 11 to 20 were prepared from the components in the weight percentages shown in table 11 (i.e., numerals in the table represent the weight percentages, e.g., 5 represents 5%), and the preparation methods of application comparative examples 11 to 20 were the same as the preparation method of the moisturizing and hydrating cream described in application example 3.

TABLE 11

Test example 5

Research on influence of human skin roughness and scale level

The dry skin is often accompanied by raised tiny scaly dandruff in the grain gaps, the skin looks dry and rough, for example, the dandruff is more serious due to excessive dryness of the skin, so that the skin feels increased in frictional resistance and obvious in rough feeling.

in the test, skin detectors such as Visia and the like are adopted to analyze the roughness and the scale level of human skin. The rougher the skin, the larger the roughness parameter; the lower the skin scale level, the lower the scale index value and the less skin scale. The test was as follows:

160 subjects who reported self-report dry skin were selected and divided into 10 groups, and the moisturizing and hydrating essence prepared in application example 2 was applied to the inner side of the left forearm of 10 groups of subjects, and the moisturizing and hydrating essence prepared in application comparative examples 1 to 10 was applied to the inner side of the right forearm of 10 groups of subjects in the same amount, respectively. The roughness and skin scale level of the same part of the inner skin of the forearm of the subject were tested before, 5min and 2h after application, respectively. The results of the human skin roughness test of this test are shown in table 12 and fig. 1, and the results of the skin scale level test are shown in table 13 and fig. 2:

TABLE 12

Watch 13

From the test results, after the application comparative example 1 (the moisturizing and moisturizing essence without the traditional Chinese medicine composition) is used for 5min, the rough and scurf states of the skin are obviously relieved due to instant exogenous moisturizing, but after 2h, the roughness parameter of the skin and the skin scale index are increased along with the evaporation of water in the essence matrix, the skin becomes dry and rough again, and the scurf is prominent. After 5min, the skin roughness parameter is reduced and the skin scale index is obviously reduced, and the skin roughness parameter and the scale index are still kept at a lower level after 2h along with the lapse of time after the application example 2 (the moisturizing essence containing 5% of the traditional Chinese medicine composition prepared in the example 1) is used, so that the effects of effectively smoothing upwarping scales and improving rough skin are realized, the skin is kept moist and smooth, and the moisturizing essence is obviously different from a matrix group.

In addition, after the essence of application example 2 is used for 2 hours, the roughness parameter and the skin scale index of the essence are greatly lower than those of application comparative examples 2-10, which fully shows that the traditional Chinese medicine components of the traditional Chinese medicine composition supplement each other and are synergistic, so that the traditional Chinese medicine composition can effectively moisturize and replenish water, relieve dryness and improve the moistening degree and smoothness of skin for a long time.

Test example 6

Research on influence of human skin overall state

When the problem of dry skin of a human body is solved, the whole state of the skin, such as the moisture, roughness, pruritus, luster, elasticity and the like, can be improved to a certain extent.

the test adopts the mode of self evaluation of the testee to evaluate the effect of the traditional Chinese medicine composition on the skin state of the human body.

The test was as follows:

176 subjects who reported dry skin were randomly and evenly divided into 11 groups, one group was used for the whole face with the moisturizing cream prepared in application example 3 of the present specification, and the other 10 groups were used with the moisturizing creams prepared in application examples 11 to 20 of the present specification for 4 weeks, and questionnaires were filled to evaluate the effects of the products, and the results of the study on the influence of the overall state of human skin are shown in table 14 and fig. 3.

TABLE 14

as can be seen from the test results shown in fig. 3 and table 14, application example 3 (moisturizing and moisturizing cream containing 5% of the traditional Chinese medicine composition) has a strong improvement effect on skin moisture, roughness, softness, elasticity, gloss and the like, the trial effective rate of the population is over 50%, wherein the trial effective rate of the population is as high as over 75%, and the trial effective rate of the population is over 75%; the effective rate of the moisturizing and hydrating cream prepared by applying the comparative examples 11-20 on a trial basis is below 30%, and the moisturizing and hydrating cream has low or even no improvement effect on the aspects of skin moisture, roughness, softness, elasticity, gloss and the like, so that the traditional Chinese medicine composition fully supplements each other and has a strong synergistic effect.

the applicant states that the present invention is illustrated by the above examples of the process of the present invention, but the present invention is not limited to the above process steps, i.e. it is not meant that the present invention must rely on the above process steps to be carried out. It will be apparent to those skilled in the art that any modification of the present invention, equivalent substitutions of selected materials and additions of auxiliary components, selection of specific modes and the like, which are within the scope and disclosure of the present invention, are contemplated by the present invention.

Claims

1. A traditional Chinese medicine composition with moisturizing and water replenishing effects is characterized in that raw materials of the traditional Chinese medicine composition comprise okra, cactus, centella asiatica, raspberry and tremella.

2. The traditional Chinese medicine composition with the effects of moisturizing and supplementing water according to claim 1, which is prepared from the following raw materials in percentage by weight: 1-30% of okra, 1-30% of cactus, 1-30% of centella, 1-30% of raspberry and 1-30% of tremella;

3. The traditional Chinese medicine composition with the effects of moisturizing and supplementing water according to claim 1 or 2, which is prepared from the following raw materials in percentage by weight: 30% of okra, 30% of cactus, 20% of centella, 10% of raspberry and 10% of tremella;

preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 25% of okra, 25% of cactus, 20% of centella asiatica, 15% of raspberry and 15% of tremella;

4. The preparation method of the traditional Chinese medicine composition with the effects of moisturizing and supplementing water according to any one of claims 1 to 3, wherein the preparation method comprises the following steps:

5. The method as claimed in claim 4, wherein the mixed powder of step (1) has a mesh size of 100-200 meshes.

6. the preparation method according to claim 4 or 5, wherein the mass ratio of the mixed powder to the solvent in the step (2) is 1 (2-20), preferably 1 (10-20);

Preferably, the solvent in the step (2) comprises any one or a mixture of at least two of water, butanediol, propanediol or ethanol, preferably water or ethanol;

preferably, the volume ratio concentration of the ethanol is 1-10%;

Preferably, the temperature of the extraction in the step (2) is 45-85 ℃;

preferably, the extraction times in the step (2) are 2-5 times, preferably 3 times;

Preferably, the time of each extraction in the extraction of the step (2) is 1-3 h;

Preferably, the concentration in step (2) comprises the following specific steps: mixing the extractive solutions, filtering, and concentrating;

Preferably, the concentration of step (2) is concentration under reduced pressure;

Preferably, the specific steps of the precipitation in the step (2) are as follows: adding a solvent into the concentrated extracting solution, carrying out fractional precipitation, and carrying out centrifugal collection;

Preferably, the solvent is ethanol;

preferably, the rotation speed of the centrifugal collection is 3000-5000 r/min;

Preferably, the drying of step (2) is vacuum drying;

preferably, the drying temperature in step (2) is 50-70 ℃, preferably 60 ℃.

7. The method according to any one of claims 4 to 6, wherein the solvent of step (3) comprises any one of water, butylene glycol or propylene glycol or a mixture of at least two thereof, preferably water and/or butylene glycol;

Preferably, the mass ratio of the water to the butanediol is 1 (0.25-2), preferably 1: 1;

Preferably, the water is deionized water;

8. The method according to any one of claims 4 to 7, characterized in that it comprises the steps of:

9. use of the Chinese medicinal composition with moisturizing and hydrating effects of any one of claims 1 to 3 in the preparation of cosmetics.

10. A cosmetic comprising the traditional Chinese medicine composition with moisturizing and hydrating effects of any one of claims 1 to 3;

Preferably, the addition amount of the traditional Chinese medicine composition with the moisturizing and water replenishing effects is 0.05-20% of the total weight of the cosmetic, and is preferably 0.1-10%;