CN110521565B - Air culture propagation method for Lanzhou lily scales - Google Patents

Air culture propagation method for Lanzhou lily scales Download PDF

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CN110521565B
CN110521565B CN201910875826.5A CN201910875826A CN110521565B CN 110521565 B CN110521565 B CN 110521565B CN 201910875826 A CN201910875826 A CN 201910875826A CN 110521565 B CN110521565 B CN 110521565B
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scales
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lily
propagation
air
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CN110521565A (en
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唐楠
唐道城
巨秀婷
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Qinghai University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

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Abstract

The invention belongs to the field of plant propagation, and particularly relates to an air-assisted cultivation propagation method for Lanzhou lily scales, which comprises the steps of design of an assisted cultivation chamber, disinfection treatment of the assisted cultivation chamber, collection and treatment of the scales, tray filling of the scales, environmental control of the assisted cultivation, assisted cultivation management and the like, and has the advantages of high propagation coefficient, short propagation time, small floor area and no influence of seasons on propagation, and overcomes the defects of long propagation period, large floor area, low propagation efficiency and difficult management of a field; overcomes the defects of large floor area, difficult environmental control and incapability of meeting the requirement of large-scale propagation in the cutting propagation.

Description

Air culture propagation method for Lanzhou lily scales
Technical Field
The invention belongs to the field of plant propagation, and particularly relates to an air-catalyzed propagation method for Lanzhou lily scales.
Background
Lily is a multifunctional economic plant integrating ornamental, edible and medicinal functions. The lily can be further divided into three types of edible lily, medicinal lily and ornamental lily according to the main application value. In recent years, with the improvement of living standard of people, the pursuit of health food is gradually upgraded, and medicinal and edible lily is mixed together to form medicinal and edible lily. The medicinal and edible lily mainly comprises Lanzhou lily (Lilium davidi var unicolor cotton), Lilium crispum (medicinal lily) (L.lancifolium) and Lilium Brownii (L.brownnii), which belong to perennial bulb herbaceous plants of Lilium (Liliacea) and Lilium of Liliaceae, and are formed by cohesion of dozens of fleshy scales.
Lanzhou lily is mainly distributed in the cold and dry mountainous areas in northwest, the Lilium tigrinum is mainly distributed in the areas of Jiangsu Yixing, Chongqing Qianjiang, Zhejiang and Guizhou, and the Longya lily is mainly distributed in the areas of Hunan, Jiangxi and Hubei. The Lanzhou lily has high sugar content, less crude fiber and fine and smooth meat quality, is a vegetable with higher nutritional and health-care values, and also has the medicinal values of clearing heat and detoxicating, tonifying lung and nourishing yin, moistening lung and beautifying, relieving cough and reducing sputum, clearing heart and tranquilizing mind, stopping bleeding and relieving pain, stopping lacrimation, resisting cancer and the like. The lily bulb is rich in nutrient substances, is rich in protein, fat, polysaccharide, vitamins and the like, and the polysaccharide is one of main functional substances in the lily and has various effects of resisting tumors, improving immunity and the like. In addition, the health-care tea also contains various mineral elements, alkaloids, steroid saponins and other components, and has an important effect on human health care. Lanzhou lily is well known as a famous dish due to its sweet and fragrant taste, is a famous Chinese dish with unique flavor, is suitable for frying, stir-frying, steaming, frying, boiling, stewing and the like, and is a precious product in Chinese dishes.
Lanzhou lily has a history of over 130 years in Qinghai and Gansu planting, is mainly distributed in Gansu, Qinghai, Ningxia, Xinjiang and the like at present, has a production area of about 20 ten thousand mu, and is centrally distributed in Guanshan town, Qinghai Min and county, Ledu district and Ping-an district of Qiriheqing, Yuzhong county, Lin Ying county and Yongjing county with the maximum production area of Gansu. In recent years, the production area of the Lanzhou lily is continuously enlarged, the production area is rapidly developed, however, the yield and the quality are sharply reduced, the phenomena that the production area is increased, the total yield and high-quality commodities are not increased are formed, the market is seriously required to be larger than the supply, and the price is continuously increased.
At present, more dilemma exists in the production and development of Lanzhou lily: the method has the advantages that the variety is single, the aging and the degradation are serious, the yield and the quality are reduced sharply, the yield is reduced from 1300 kg/mu-1400 kg/mu of fresh bulb harvesting amount per unit area to 600 kg/mu-900 kg/mu in 1987, and the reduction range is 30% -50%; secondly, the distribution of the production area is narrow, the continuous cropping phenomenon is serious, the quality is poor, farmers grow in generations, crop rotation and stubble rotation cannot be realized on limited land, the continuous cropping of only one year after another is driven by economic benefits, or the continuous cropping is simply carried out in a short period, so that the balance of soil nutrients is broken, the plant diseases and insect pests are frequent and serious, the nutritional quality of bulbs is reduced, the yield is reduced, and even the phenomenon of no harvest occurs in the old planting area; the traditional seed reserving and production mode is universal, the specialized and standardized production level is deficient, the high-quality product rate is low, the stem miniature bulbs and the non-commercial miniature bulbs are adopted for seed production, the seed source quality is poor, the product quality is poor, the 'thousand-head' lily bulbs are more and more, the single-head lily is less and less, and the proportion of the exported product is reduced; the method has the advantages that the method is lack of specialized production and supply of high-quality provenance, rejuvenescence of varieties, severe disjunction of new variety breeding and industrial development, seed reservation for self-growing, seed reservation for stem growing and non-commercial ball, seed reservation for high generation and the like become main provenance, so that viral diseases and fungal diseases are severe, seed degradation is accelerated, and finally, the yield is low, the quality is poor, the commodity rate is low, the number of high-quality products is small, the export products are contracted, the income is reduced, and the enthusiasm of farmer production is low.
At present, the defects of long breeding period, large occupied area, low breeding efficiency and difficult management of the field are overcome. Overcomes the defects of large floor area, difficult environmental control and incapability of meeting the requirement of large-scale propagation in the cutting propagation.
Disclosure of Invention
The invention aims to provide an air-catalyzed propagation method of Lanzhou lily scales, which aims to overcome the defects of the prior art, reduce the occupied area, shorten the propagation time and improve the propagation rate.
In order to achieve the purpose, the invention is realized by the following technical scheme:
an air-catalyzed propagation method of Lanzhou lily scales comprises the following steps:
s101, designing a culture room, placing culture frames in the culture room, wherein each layer of each culture frame is provided with an LED light supplementing lamp tube;
s102, treating in a culture room, repeatedly scrubbing various facilities in the culture room, and then spraying and disinfecting with formaldehyde solution and 84 disinfectant for multiple times;
s103, collecting scales, selecting bulbs without rot and pollution, and collecting the scales after removing outer-layer scales;
s104, processing the scales, namely selecting each layer and/or mixed scale of the lily, soaking the lily in a disinfectant for 30min, washing the lily with clear water, and draining for later use;
s105, dishing, namely placing the treated scales in sterilized culture dishes, placing 1.0kg of scales in each culture dish, covering sterile white gauze, and placing the culture dishes on a culture rack for culture;
s106, controlling the catalytic culture environment, wherein the indoor temperature is controlled to be 20-25 ℃, the relative humidity of air is controlled to be 70-90%, and the illumination intensity is controlled to be 200-500 lx;
s107 cultivation promotion management: (1) periodically disinfecting, namely disinfecting the culture room and the tools with formaldehyde liquid every week; (2) picking out suspected diseased scales: checking all the culture trays every day, and picking out lily scales suspected to be diseased; (3) and (3) observing an environmental factor: the indoor temperature, the air relative humidity and the illumination are observed every day, and the indoor temperature, the air relative humidity and the illumination are adjusted in time when the indoor temperature, the air relative humidity and the illumination do not meet the culture accelerating condition.
Further, the bulbs in S103 are single-headed bulbs or double-headed bulbs, and the weight of a single bulb is more than or equal to 100 g.
Further, the disinfectant in the S104 is a 50% thiophanate methyl solution.
Further, the scale in S104 is a lily scale collected by a hierarchical order collection method and/or a mixed weight collection method.
Further, the hierarchical order acquisition method comprises: removing the polluted scales on the outer layer from the bulb of the lily, and then sequentially peeling the lily scales from outside to inside, wherein 1-2 layers are outer-layer scales, 3-4 layers are middle-layer scales, and 5-7 layers are inner-layer scales.
Further, the mixed weight collection method comprises the following steps: after the polluted scales on the outer layer of the bulb of the lily are removed, collecting mixed scales respectively for each bulb, and classifying according to the weight of the scales, wherein the first-level scales are more than or equal to 3.0 g/piece, the second-level scales are 2.0-3.0 g/piece, the third-level scales are 1.0-2.0 g/piece, and the fourth-level scales are less than or equal to 1.0 g/piece.
Further, the lily scales in S104 are mixed scales of any one or more of outer-layer scales, middle-layer scales, inner-layer scales, primary scales, secondary scales, and tertiary scales.
Furthermore, the culture accelerating tray of S105 is a hard plastic seedling raising tray of 63.0cm multiplied by 30.0cm multiplied by 3.0cm, and through holes with the interval of 1.0cm multiplied by 1.0cm and the diameter of 0.2cm are arranged at the tray bottom.
Preferably, the illumination intensity in S106 is 400 lx.
Further, S101 the incubation chamber in be provided with electric heater unit, environment humiture monitor and carbon dioxide concentration detector, electric heater unit, environment humiture monitor and carbon dioxide concentration detector be connected with the master control box.
Further, the sidewall of the culture catalysis chamber in the step S101 is provided with a ventilation fan, and the ventilation fan is connected with the main control box.
Furthermore, the culture room in S101 is at least provided with a light-transmitting wall.
Furthermore, each layer of each group of culture racks in the step S101 is respectively provided with a mist spray head.
Compared with the prior art, the air-catalyzed propagation method for Lanzhou lily scales provided by the invention has the following effects:
(1) the propagation coefficient is high: the medium-free culture is a propagation mode with the highest scale differentiation rate, scale propagation coefficient, bulb propagation rate and propagation efficiency, the propagation mode is 181.9% higher than that of conventional field sowing propagation, the unit bulb propagation coefficient reaches 130.8 times per bulb, and the propagation mode is 150.45% higher than that of cutting propagation.
(2) The occupied area is small: the quality required by cutting propagation and field direct seeding scale is higher than that required by medium-free propagation, so that the propagation floor area of the air propagation technology is smaller than that of cutting propagation and field seeding propagation, and high-yield propagation can be realized in a smaller volume.
(3) The propagation time is short: the time of at least 13 weeks from sowing to seedling emergence and bulblet formation in the field scale direct seeding propagation is required, the time of about 9 weeks is also required for cutting propagation, and the time of 7 weeks is required at most in the air culture method, so that the propagation efficiency can be greatly improved, and the propagation time is also shortened;
(4) propagation is not affected by season: the air culture method is not affected by seasonal changes, can be used for breeding production all the year round, is suitable for high-efficiency and rapid breeding of high-quality seed sources, and is also suitable for generation-adding breeding of a small amount of bulbs of lily breeding workers;
(5) the medium-free scale culture propagation is combined with the scale grading standard, so that the propagation efficiency and the quality of the miniature bulbs are greatly improved, the disease incidence of diseases and viruses is favorably reduced, the yield, the quality and the commodity rate of the commodity lily are improved, the economic benefit is increased, and the large-scale rapid development of the Lanzhou lily industry is promoted.
In conclusion, the air culture propagation method provided by the invention ensures a certain environmental temperature and air relative humidity, and does not add any physical and chemical elements, so that an environment suitable for the differentiation of the scale into the miniature bulb is created, and the quick formation of the miniature bulb is promoted. The method overcomes the defects of long breeding period, large floor area, low breeding efficiency and difficult management of the field; overcomes the defects of large floor area, difficult environmental control and incapability of meeting the requirement of large-scale propagation in the cutting propagation.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the following embodiments are described in further detail, which are used for illustrating the present invention but not for limiting the scope of the present invention.
An air-catalyzed propagation method of Lanzhou lily scales comprises the following steps:
s101, designing a culture room, placing culture frames in the culture room, wherein each layer of each culture frame is provided with an LED light supplementing lamp tube;
s102, treating in a culture room, repeatedly scrubbing various facilities in the culture room, and then spraying and disinfecting with formaldehyde solution and 84 disinfectant for multiple times;
s103, collecting scales, selecting bulbs without rot and pollution, and collecting the scales after removing outer-layer scales;
s104, processing the scales, namely selecting each layer and/or mixed scale of the lily, soaking the lily in a 50% thiophanate methyl solution for 30min, washing the lily with clear water, and draining for later use;
s105, carrying out scale dishing, placing the processed scales in sterilized culture dishes, placing 1.0kg of scales in each culture dish, covering with sterile white gauze, and placing on a culture rack for culture, wherein the culture dishes are hard plastic seedling raising dishes of 63.0cm multiplied by 30.0cm multiplied by 3.0cm, and through holes of 1.0cm multiplied by 1.0cm and 0.2cm in diameter are arranged at the bottoms of the dishes.
S106, controlling the culture accelerating environment, and comprehensively evaluating the suspected morbidity, the differentiation time of the micro bulbs, the differentiation speed, the differentiation quantity, the secondary rooting development, the bud development, the micro bulbs and the like, wherein the optimal temperature of the medium-free culture accelerating is 20-25 ℃. Therefore, the indoor temperature is controlled to be 20-25 ℃, the relative humidity of air is 70-90%, and the illumination intensity is 200-500 lx;
s107 cultivation promotion management: (1) periodically disinfecting, namely disinfecting the culture room and the tools with formaldehyde liquid every week; (2) picking out suspected diseased scales: checking all the culture trays every day, and picking out lily scales suspected to be diseased; (3) and (3) observing an environmental factor: the indoor temperature, the air relative humidity and the illumination are observed every day, and the indoor temperature, the air relative humidity and the illumination are adjusted in time when the indoor temperature, the air relative humidity and the illumination do not meet the culture accelerating condition.
Further, the bulbs in S103 are single-headed bulbs or double-headed bulbs, and the weight of a single bulb is more than or equal to 100 g. The single-head bulbs are preferably selected as materials, and the single-head bulbs with the weight of more than or equal to 100g are selected, so that the method is favorable for obtaining more scales, higher collection rate and better scale quality, and is also favorable for culturing high-quality micro bulbs and rejuvenating the seed properties.
Further, the scale in S104 is a lily scale collected by a hierarchical order collection method and/or a mixed weight collection method. Wherein, the hierarchical order acquisition method comprises the following steps: removing the polluted scales on the outer layer from the bulb of the lily, and then sequentially peeling the lily scales from outside to inside, wherein 1-2 layers are outer-layer scales, 3-4 layers are middle-layer scales, and 5-7 layers are inner-layer scales. The mixed weight collection method comprises the following steps: after the polluted scales on the outer layer of the bulb of the lily are removed, collecting mixed scales respectively for each bulb, and classifying according to the weight of the scales, wherein the first-level scales are more than or equal to 3.0 g/piece, the second-level scales are 2.0-3.0 g/piece, the third-level scales are 1.0-2.0 g/piece, and the fourth-level scales are less than or equal to 1.0 g/piece.
Further, the lily scales in S104 are mixed scales of any one or more of outer-layer scales, middle-layer scales, inner-layer scales, primary scales, secondary scales, and tertiary scales.
Further, S101 the foster indoor electric heater unit, environment humiture monitor and carbon dioxide concentration detector of being provided with of foster, the lateral wall of foster room is provided with the scavenger fan, electric heater unit, environment humiture monitor and carbon dioxide concentration detector be connected with the master control box. The electric heating device used in the invention is electric heating heater. The temperature automatic control ware temperature regulation in the total control box, when the temperature surpassed the settlement temperature, the scavenger fan was opened automatically for indoor outer air exchange, when the air temperature was less than the settlement temperature, heating device lasted the heating, with temperature effective control at 20 ~ 25 ℃. The environment temperature and humidity monitor is connected with the master control box controller, the controller controls the power switch of the environment temperature and humidity monitor according to indoor relative air humidity, wherein each layer of each group of culture frames is respectively provided with a mist spray nozzle, so that the indoor relative air humidity can be adjusted, and the air relative humidity is controlled at 70% -90%.
The culture room in S101 is provided with at least one light-transmitting wall, and preferably, the east-facing side wall is provided with the light-transmitting wall.
Example 1
The embodiment provides an air-catalyzed propagation method of Lanzhou lily scales, which comprises the following steps:
s101, treating in a culture room, placing culture frames in the culture room, wherein each layer of each culture frame is provided with an illumination led light supplement lamp tube, repeatedly scrubbing various facilities in the culture room, and then spraying and disinfecting with formaldehyde solution and 84 disinfectant for multiple times;
s102, processing the scales, namely selecting each-level scale of lily, soaking the scales in 50% thiophanate methyl solution for 30min, washing the scales with clear water, and draining for later use;
the scale is lily scale collected by lily bulb according to hierarchical order collection method, which comprises: removing the polluted scales on the outer layer from the bulb of the lily, and then sequentially peeling the lily scales from outside to inside, wherein 1-2 layers are outer-layer scales, 3-4 layers are middle-layer scales, and 5-7 layers are inner-layer scales. The scale for propagation is a middle layer scale and an inner layer scale.
S103, dishing, namely placing the treated scales in sterilized culture dishes, placing 1.0kg of scales in each culture dish, covering sterile white gauze, and placing the culture dishes on a culture rack for culture;
s104, controlling the relative humidity of indoor air to be 70%, controlling the indoor temperature to be 25 ℃, wherein the indoor temperature is the optimal culture temperature, and the method not only has high differentiation speed, but also has high final differentiation rate. The illumination intensity is controlled to be 400lx, the illumination intensity of about 400lx is beneficial to the differentiation of the miniature bulbs of the scales and the root differentiation, and is also beneficial to the growth inhibition of the miniature bulb buds, and the lower the illumination intensity is, the formation and the development of the miniature bulbs and the formation and the development of secondary roots are not beneficial, but the growth of the buds is obviously promoted.
S105 cultivation promotion management: (1) periodically disinfecting, namely disinfecting the culture room and the tools with formaldehyde liquid every week; (2) picking out suspected diseased scales: checking all the culture trays every day, and picking out lily scales suspected to be diseased; (3) and (3) observing an environmental factor: the indoor temperature, the relative humidity of air and the illumination are observed every day, and the adjustment is carried out when the indoor temperature, the relative humidity of air and the illumination do not meet the culture accelerating condition.
Example 2
The embodiment provides an air-catalyzed propagation method of Lanzhou lily scales, which comprises the following steps:
s101, treating in a culture room, placing culture frames in the culture room, wherein each layer of each culture frame is provided with an illumination led light supplement lamp tube, repeatedly scrubbing various facilities in the culture room, and then spraying and disinfecting with formaldehyde solution and 84 disinfectant for multiple times;
s102, processing the scales, namely selecting each-level scale of lily, soaking the scales in 50% thiophanate methyl solution for 30min, washing the scales with clear water, and draining for later use;
the scale is the scale collected by the lily bulb according to a mixed weight collection method, the collection method is that after the scale with the outer layer polluted is removed from the lily bulb, each scale is respectively collected and classified according to the weight of the scale, the first-level scale is more than or equal to 3.0 g/piece, the second-level scale is 2.0-3.0 g/piece, the third-level scale is 1.0-2.0 g/piece, and the fourth-level scale is less than or equal to 1.0 g/piece. The scale for propagation is a mixed scale of a first-level scale, a second-level scale and a third-level scale.
S103, dishing, namely placing the treated scales in sterilized culture dishes, placing 1.0kg of scales in each culture dish, covering sterile white gauze, and placing the culture dishes on a culture rack for culture;
s104, controlling the catalytic culture environment, controlling the relative humidity of indoor air to be 80%, controlling the indoor temperature to be 22 ℃, and controlling the illumination intensity to be 200 lx.
S105 cultivation promotion management: (1) periodically disinfecting, namely disinfecting the culture room and the tools with formaldehyde liquid every week; (2) picking out suspected diseased scales: checking all the culture trays every day, and picking out lily scales suspected to be diseased; (3) and (3) observing an environmental factor: the indoor temperature, the relative humidity of air and the illumination are observed every day, and the adjustment is carried out when the indoor temperature, the relative humidity of air and the illumination do not meet the culture accelerating condition.
Example 3
The embodiment provides an air-catalyzed propagation method of Lanzhou lily scales, which comprises the following steps:
s101, treating in a culture room, placing culture frames in the culture room, wherein each layer of each culture frame is provided with an illumination led light supplement lamp tube, repeatedly scrubbing various facilities in the culture room, and then spraying and disinfecting with formaldehyde solution and 84 disinfectant for multiple times;
s102, processing the scales, namely selecting each-level scale of lily, soaking the scales in 50% thiophanate methyl solution for 30min, washing the scales with clear water, and draining for later use;
the scales are lily scales collected by a hierarchical order collection method and a mixed weight collection method;
the hierarchical order acquisition method comprises the following steps: removing the polluted scales on the outer layer from the bulb of the lily, and then sequentially peeling the lily scales from outside to inside, wherein 1-2 layers are outer-layer scales, 3-4 layers are middle-layer scales, and 5-7 layers are inner-layer scales.
The mixed weight collecting method comprises the steps of removing the scales polluted on the outer layer of the bulb of the lily, collecting the mixed scales for each bulb respectively, and classifying according to the weight of the scales, wherein the first-level scales are more than or equal to 3.0 g/piece, the second-level scales are 2.0-3.0 g/piece, the third-level scales are 1.0-2.0 g/piece, and the fourth-level scales are less than or equal to 1.0 g/piece. The scale for propagation is a mixed scale of an outer layer scale, a middle layer scale, a first-level scale, a second-level scale and a third-level scale.
S103, dishing, namely placing the treated scales in sterilized culture dishes, placing 1.0kg of scales in each culture dish, covering sterile white gauze, and placing the culture dishes on a culture rack for culture;
s104, controlling the relative humidity of indoor air to be 90%, controlling the indoor temperature to be 25 ℃, wherein the indoor temperature is the optimal culture temperature, and the method not only has high differentiation speed, but also has high final differentiation rate. The illumination intensity is controlled to be 500 lx.
S105 cultivation promotion management: (1) periodically disinfecting, namely disinfecting the culture room and the tools with formaldehyde liquid every week; (2) picking out suspected diseased scales: checking all the culture trays every day, and picking out lily scales suspected to be diseased; (3) and (3) observing an environmental factor: observing indoor temperature, air relative humidity and illumination every day, adjusting when not meeting the culture accelerating condition, and specifically regulating and controlling as follows:
temperature control: the temperature control adopts a method combining automatic control, automatic detection and indoor temperature measurement, and compares actual measurement with automatic set temperature to obtain a temperature correction value, and then adjusts the temperature according to the requirement. In the culture accelerating room, a 2000W oil tincture type electric heating device (electric heating heater) is installed, 6 groups of heaters are installed in total, each group is connected with a master control box, and an electric heater switch is adjusted by an automatic temperature controller in the master control box. When the temperature exceeds the set temperature, the ventilation fan is automatically opened, and indoor and outdoor air exchange is accelerated. When the air temperature is lower than the set temperature, the heater continuously heats, the temperature is effectively controlled to be 20-255 ℃, and a plurality of sets of environment temperature and humidity monitors are arranged indoors to correct the self-controlled temperature.
And (3) illumination control: the lily scales are modified leaves and have the characteristic of photosynthesis of leaves under light, so that certain illumination intensity is favorable for promoting the formation of micro bulbs on the scales, the illumination of the air culture room is supplemented by taking different layers of the culture racks as units, each layer of each culture rack is provided with led light supplement lamp tubes with the volume of more than or equal to 1000lux except a total light source for illumination in the culture rack, and the light parameters are regulated and set in a split rack mode.
Air relative humidity control: the relative humidity of the ambient air is an important factor for air culture of the scales, and the differentiation rate, the number of micro bulbs, the suspected morbidity and the like of the scales are related to the ambient humidity. The invention divides the regulation and control of the environmental humidity into three parts: the first part of water source is a 2000L water storage plastic tank, tap water is introduced into the plastic tank, and the water is used for scale spraying after being stored for 24 hours; the second part is water supply power, a water supply tank is placed at a high place of 200.0 cm, pressurization is carried out by using gravity, water supplement on the surface of the scale under low pressure is realized, and a 500W submersible pump, namely a pressurization pump for regulating and controlling the environmental humidity, is installed in a water storage tank; the third part is an environment humidity regulating device, each layer of each group of culture racks is respectively provided with a spray nozzle which is conducted to a control box through a humidity sensor, and an automatic controller starts a power switch according to the interlayer humidity to supplement the relative humidity of air, so as to ensure that the relative humidity of the air can be regulated to 70.0-90.0%.
Example 4
And (5) comparing and testing the cultivation effect.
And (3) measurement of morphological indexes:
culturing according to the embodiment 1-3, observing data after culturing 7w, randomly selecting 100 pieces for each treatment, repeating the three times, averaging, and recording the data. The observation indexes are as follows:
(1) suspected incidence of scale: the water soaked spots, the rotten signs of softening or the burnt yellow spots are provided, and the later stage partial morbid state disappears or is converted into the symptoms of fungal or bacterial infection. The ratio of the number of scales of suspected diseases visible to the naked eye to the total number of scales of the spot test is expressed in percentage. Calculating the formula: the suspected incidence of scale is equal to the number of suspected scales/100 × 100%.
(2) Scale differentiation rate: counting and counting the number of scales of the growing micro bulb,
the scale differentiation rate is the number of scales of the long bulb/100 pieces × 100%.
(3) Propagation coefficient of micro scale: the number of micro bulbs growing on each bulb.
(4) Scale breeding efficiency: the number of bulbs bred by a single scale is calculated according to the formula: the scale breeding efficiency is equal to the total number of the miniature bulbs/100 multiplied by 100 percent.
The results of the morphological index measurements are shown in Table 1.
Table 1 comparative experiment results of cultivation effect
Figure BDA0002204248430000101
The results of comparing the conventional breeding patterns disclosed according to the test and the prior art with the method provided by the present invention are shown in table 2.
TABLE 2 comparison of the propagation differences of Lily leaves in Lanzhou
Figure BDA0002204248430000102
As can be seen from the above table, air culture is a breeding mode with the highest differentiation rate, propagation coefficient, bulb propagation rate and propagation efficiency of the scales. Firstly, compared with the conventional field sowing and propagation, the air-assisted culture is improved by 187.37%, and the unit bulb propagation coefficient reaches 130.8 times per bulb, and is improved by 150.45% compared with the cutting propagation. The quality required by cutting propagation and field seeding of the crystalline flakes is higher than that required by air culture; secondly, the air-catalyzed propagation method occupies a smaller area than the land for sowing propagation and cutting propagation, the sowing quantity of the air-catalyzed propagation unit area is increased by at least 92 times compared with the land for sowing propagation and 11.5 times compared with the cutting propagation, and the land utilization rate is greatly increased; moreover, the propagation time of air culture is short, at least 13 weeks are needed from sowing to seedling emergence and bulblet formation in field sowing, about 9 weeks are needed for cutting propagation, at most 7 weeks are needed for air culture, the propagation efficiency can be greatly improved, the propagation time and the propagation period are also shortened, and meanwhile, the propagation mode is not influenced by seasonal changes, can be used for propagation production all the year round, is suitable for high-quality provenance efficient rapid propagation, and is also suitable for generational propagation of a small amount of bulblets of lily breeding workers; finally, the suspected morbidity of the scales is very low by the air culture method, and the suspected morbidity of the scales of each level is lower than 2.0% by the time of finishing the incubation (7 weeks).
The method overcomes the defects of long breeding period, large floor area, low breeding efficiency and difficult management in the field. Overcomes the defects of large floor area, difficult environmental control and incapability of meeting the requirement of large-scale propagation in the cutting propagation.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.

Claims (6)

1. An air-catalyzed propagation method of Lanzhou lily scales is characterized by comprising the following steps:
s101, designing a culture room, placing culture frames in the culture room, wherein each layer of each culture frame is provided with an LED light supplementing lamp tube and a mist spray head, an electric heating device, an environment temperature and humidity monitor and a carbon dioxide concentration detector are arranged in the culture room, the electric heating device, the environment temperature and humidity monitor and the carbon dioxide concentration detector are connected with a main control box, and the culture room is at least provided with a light-transmitting wall;
s102, treating in a culture room, repeatedly scrubbing various facilities in the culture room, and then spraying and disinfecting with formaldehyde solution and 84 disinfectant for multiple times;
s103, collecting scales, selecting single-end bulbs or double-end bulbs without rot and pollution, wherein the weight of a single bulb is more than or equal to 100g, and collecting the scales after removing outer-layer scales;
s104, processing the scales, namely selecting each layer and/or mixed scale of the lily, soaking the lily in 50% thiophanate methyl solution serving as a disinfectant for 30min, washing the lily with clear water, and draining the lily for later use; the mixed scale is collected by a mixed weight collection method, and the collection method comprises the following steps: after removing the polluted scales on the outer layer of the bulb of the lily, collecting mixed scales respectively for each bulb, and classifying according to the weight of the scales, wherein the first-level scales are more than or equal to 3.0 g/piece, the second-level scales are 2.0-3.0 g/piece, the third-level scales are 1.0-2.0 g/piece, and the fourth-level scales are less than or equal to 1.0 g/piece;
s105, dishing, namely placing the treated scales in sterilized culture dishes, placing 1.0kg of scales in each culture dish, covering sterile white gauze, and placing the culture dishes on a culture rack for culture;
s106, controlling the catalytic culture environment, wherein the indoor temperature is controlled to be 20-25 ℃, the relative humidity of air is controlled to be 70-90%, and the illumination intensity is controlled to be 200-500 lx;
s107 cultivation promotion management: (1) periodically disinfecting, namely disinfecting the culture room and the tools with formaldehyde liquid every week; (2) picking out suspected diseased scales: checking all the culture trays every day, and picking out lily scales suspected to be diseased; (3) and (3) observing an environmental factor: the indoor temperature, the air relative humidity and the illumination are observed every day, and the indoor temperature, the air relative humidity and the illumination are adjusted in time when the indoor temperature, the air relative humidity and the illumination do not meet the culture accelerating condition.
2. The air culture propagation method of Lanzhou lily scales as claimed in claim 1, which is characterized in that: the scales in the S104 are lily scales collected by lily bulbs according to a hierarchical order collection method and/or a mixed weight collection method.
3. The air culture propagation method of Lanzhou lily scales as claimed in claim 1, which is characterized in that: the scales of all levels of the lily are scales acquired by a hierarchical order acquisition method, and the acquisition method comprises the following steps:
removing the polluted scales on the outer layer from the bulb of the lily, and then sequentially peeling the lily scales from outside to inside, wherein 1-2 layers are outer-layer scales, 3-4 layers are middle-layer scales, and 5-7 layers are inner-layer scales.
4. The air culture propagation method of Lanzhou lily scales as claimed in claim 3, which is characterized in that: s104, the lily scales are any one or a mixture of a plurality of outer-layer scales, middle-layer scales, inner-layer scales, first-level scales, second-level scales and third-level scales.
5. The air culture propagation method of Lanzhou lily scales as claimed in claim 1, which is characterized in that: s105, the culture accelerating tray is a hard plastic seedling raising tray of 63.0cm multiplied by 30.0cm multiplied by 3.0cm, and through holes with the interval of 1.0cm multiplied by 1.0cm and the diameter of 0.2cm are arranged at the bottom of the tray.
6. The air culture propagation method of Lanzhou lily scales as claimed in claim 1, which is characterized in that: and the side wall of the culture accelerating chamber is provided with an air exchange fan, and the air exchange fan is connected with the master control box.
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