CN110122087A - The method of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture - Google Patents
The method of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture Download PDFInfo
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- CN110122087A CN110122087A CN201910568209.0A CN201910568209A CN110122087A CN 110122087 A CN110122087 A CN 110122087A CN 201910568209 A CN201910568209 A CN 201910568209A CN 110122087 A CN110122087 A CN 110122087A
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- Prior art keywords
- cuttage
- fructus citri
- citri sarcodactylis
- cuttings
- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of methods of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, wherein, it include: the cuttings for selecting semi-lignified, using NAA 200mg/L solution to cuttings immersion treatment 15 minutes, then cuttage is into the cutting medium for using nursery soil and coco bran to prepare by weight=1:1, then carries out the management after cuttage and obtain the FRUCTUS CITRI SARCODACTYLIS seedling that can be used for transplanting.The rooting rate for the FRUCTUS CITRI SARCODACTYLIS seedling that method of the invention is cultivated is high, reaches 94% or more, and high survival rate, production cost is low, and condition of rooting is good, and reproduction speed is enhanced about more than once, and seedling quality is high, has good market prospects；And growing-seedling period is short, yield is big, less energy consumption, is conducive to extensive nursery.
Description
Technical field
The present invention relates to FRUCTUS CITRI SARCODACTYLIS seedling-raising technique fields, it is more particularly related to a kind of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture
Method.
Background technique
FRUCTUS CITRI SARCODACTYLIS is Rutaceae citrus plant Citrus medica L.var.sarcodactylis (Noot.)
Swingle ripening fruits has qi-regulating promoting the circulation of qi, and preventing or arresting vomiting stomach function regulating, invigorating the spleen open the functions such as strongly fragrant, resolving sputum, can treat for stomachache, stagnant gas, abdomen in the heart
Portion's turgor, the diseases such as accumulation of stopping eating.For FRUCTUS CITRI SARCODACTYLIS in addition to medicinal, also extractable essence is processed into fingered citron sugar, candied fruit, fingered citron solution
The health foods such as wine tea, fingered citron habit-breaking candy for smokers.
Current main FRUCTUS CITRI SARCODACTYLIS propagation method is cutting propagation, needed 2 years by normal cutting propagation or more, the period
It is long, seriously affect plantation period.And traditional cutting propagation is to cuttings, cutting medium, hormone kind, concentration and soaking time
It selects improper, leads to thousand fingered citron low seedling survival rates.
Therefore, the quick breeding for finding new technology for FRUCTUS CITRI SARCODACTYLIS is current urgent problem to be solved, efficient cuttage and quick-propagation
Help to improve the nursery success rate of FRUCTUS CITRI SARCODACTYLIS.
Summary of the invention
It is an object of the invention to solve at least the above defect, and provide the advantages of at least will be described later.
It is a further object to provide a kind of methods of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture that can be improved survival rate of seedling.
It is a further object to provide the methods that one kind can shorten the FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture of growing-seedling period.
In order to realize these purposes and other advantages according to the present invention, the present invention provides a kind of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture
Method, wherein include:
The cuttings for selecting semi-lignified, using NAA 200mg/L solution to cuttings immersion treatment 15 minutes, then by cuttings
Cuttage finally carries out managing after cuttage obtaining into the cutting medium prepared by nursery soil and coco bran by weight=1:1
It can be used for the FRUCTUS CITRI SARCODACTYLIS seedling transplanted.
FRUCTUS CITRI SARCODACTYLIS survival rate of seedling is improved by the selection of cuttings, processing, the preparation of cutting medium, solves traditional skill
Cuttings cuttage is directly carried out to the low problem of survival rate caused by nursery in art in soil.
Preferably, in the method for the FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, management includes: after the cuttage
After cuttings cuttage to cutting medium, control shading rate is 75%, and control air themperature is 25 DEG C, air humidity 40
~60%；In cuttage 40 days, control cutting medium humidity is 70~75%；
After cuttage 40 days, control cutting medium humidity is 55~65%, 80% or more air humidity, and keeps ventilation saturating
Gas, cuttage obtain the FRUCTUS CITRI SARCODACTYLIS seedling that can be used for transplanting after 120 days.
By carrying out the management after cuttage to the FRUCTUS CITRI SARCODACTYLIS cuttings after cuttage, FRUCTUS CITRI SARCODACTYLIS growing-seedling period is shortened, is solved
The long technical problem of FRUCTUS CITRI SARCODACTYLIS growing-seedling period existing in the prior art.
Preferably, in the method for the FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, the selection of the cuttings specifically:
Take 3~8 years FRUCTUS CITRI SARCODACTYLIS current year sprouting growth 6~10 months diameter 1.0cm or more to semi-lignified of growth cycle
Healthy and strong no disease and pests harm branch, gained branch is cut into the fringe bar of 14~16cm, removes fringe bar leaf, keeps sheared edge smooth
Obtain the cuttings.
Preferably, in the method for the FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, after removing fringe bar leaf, it is also necessary to use quality point
The potassium permanganate of number 0.5% carries out spray disinfectant to fringe bar, then rinses fringe bar water well and just obtains the cuttings.
Preferably, in the method for the FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, 15 are impregnated to cuttings using NAA 200mg/L solution
It is after minute, cuttings washing is clean spare.
Preferably, in the method for the FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, the cutting medium the preparation method comprises the following steps:
It carries out nursery soil and coco bran to be mixed to get cutting medium with weight ratio=1:1, then the cutting medium is sprayed
10% carbendazim solution of concentration carries out disinfection, and spray suitable water so that the water content of the cutting medium reach 40~
60%.
Preferably, in the method for the FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, the nursery soil is turfy soil, vermiculite and perlite
It is mixed to get with the mass ratio of 4:1:1.
The present invention is include at least the following beneficial effects:
Method of the invention passes through efficient skewer by managing after the selection of cuttings, processing, the preparation of cutting medium, cuttage
Slotting technology solves the technical problem that FRUCTUS CITRI SARCODACTYLIS growing-seedling period existing in the prior art is long, and survival rate is low, yield is not high.
After method of the invention, the rooting rate of FRUCTUS CITRI SARCODACTYLIS seedling is high, reaches 94% or more, high survival rate, production cost
Low, condition of rooting is good, and reproduction speed is enhanced about more than once, and seedling quality is high, has good market prospects, and growing-seedling period
Short, yield is big, less energy consumption, is conducive to extensive nursery.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
Embodiment 1
Cuttings selects and processing: the semi-lignified stalwartness no disease and pests harm of growth cycle FRUCTUS CITRI SARCODACTYLIS current growth in 3 years 6 months is taken
Branch as cuttings, be cut into 15cm or so, remove fringe bar leaf, notch is smooth, the immersion treatment in NAA 200mg/L solution
15min, clear water are rinsed well spare.
The preparation in seedbed: nursery soil and coco bran are mixed to get cutting medium by weight=1:1, are filled using cutting medium
Seedbed, seedbed are made into furrow, furrow high 15cm, seeding row spacing 15cm × 20cm；Cutting medium sprays 10% using spraying device before cuttage
Carbendazim solution disinfection, then cutting medium is irrigated to moisture content 50% using watering can, held with hand can be discharged but not at stream i.e.
It can.The nursery soil is that turfy soil, vermiculite and perlite are mixed to get with the mass ratio of 4:1:1.
Cuttage cuttings: cutting propagation period selected in early or mid November；Cuttings morphology lower end is inserted into cutting medium
In, cuttage depth 5cm.
It is managed after cuttage: selecting the sunshade net of shading rate 75% to carry out shading after cuttage, control air themperature is 25 DEG C, empty
Air humidity degree is 40~60%；In cuttage 40 days, after control cutting medium humidity is 70~75%, cuttage 40 days, cuttage base is controlled
Matter humidity is 55~65%, 80% or more air humidity, and keeps ventilated, and cuttage is transplanted after 120 days.
Embodiment 2
Cuttings selects and processing: the growth 10 months semi-lignified stalwartness nothings that 8 years FRUCTUS CITRI SARCODACTYLIS current years of growth cycle are raw are taken
The branch of pest and disease damage is cut into 15cm or so as cuttings, removes all leaves in fringe bar, and notch is smooth, with 0.5% permanganic acid
Potassium carries out spray disinfectant, and flowing water is rinsed well, and the immersion treatment 15min in NAA 200mg/L solution, clear water is rinsed well standby
With.
The preparation in seedbed: nursery soil and coco bran are mixed to get cutting medium by weight=1:1, are filled using cutting medium
Seedbed, seedbed are made into furrow, furrow high 15cm, seeding row spacing 15cm × 20cm；Cutting medium sprays 10% using spraying device before cuttage
Carbendazim solution disinfection, then cutting medium is irrigated to moisture content 50% using watering can, held with hand can be discharged but not at stream i.e.
It can.The nursery soil is that turfy soil, vermiculite and perlite are mixed to get with the mass ratio of 4:1:1.
Cuttage cuttings: cutting propagation period selected in early or mid November；Cuttings morphology lower end is inserted into cutting medium
In, cuttage depth 7cm.
It is managed after cuttage: selecting the sunshade net of shading rate 75% to carry out sunshade after cuttage, control air themperature is 25 DEG C, empty
Air humidity degree is 40~60%；In cuttage 40 days, after control soil humidity is 70~75%, cuttage 40 days, control soil humidity is
55~65%, 80% or more air humidity, and keep ventilated, cuttage is transplanted after 120 days.
Experimental Comparison analysis
Test 1
The unified method using in embodiment 1, difference, which is only that, selects different cuttings, tests different degrees of lignification and inserts
Influence of the fringe (tender cuttings, semi-wooden scion, lignifying cuttings) to cuttage root-taking uses randomized block experiment, 3 repetitions, knot
Fruit is as shown in table 1 below.
Influence of the table 1- difference degree of lignification cuttings to cuttage root-taking
As can be seen from Table 1, different degree of lignification cuttings have a significant impact FRUCTUS CITRI SARCODACTYLIS cuttage root-taking, wherein selecting
The rooting rate of semi-wooden scion is up to 88.65%, and followed by lignifying cuttings is 76.23%, and last is that tender cuttings is
62.58%, reach extremely significant difference between three.
Quantity of taking root is up to 20.8 with semi-wooden scion, and followed by tender cuttings reaches extremely significant difference between the two
It is different, it is finally lignifying cuttings.
Root long is for up to 6.44cm with semi-wooden scion, followed by tender cuttings, between the two without significant difference.
Root thickness are up to 1.03mm with lignifying cuttings, followed by semi-wooden scion, no significant difference between two, minimum
Be tender cuttings be 0.56mm, reach extremely significant difference with semi-wooden scion root thickness.
Therefore, in general the method choice semi-wooden scion of FRUCTUS CITRI SARCODACTYLIS cuttage seeding of the invention can obtain significantly
Effect.
Test 2
Based on the method in embodiment 1, test 2 sets cutting medium (A), hormone kind (B), hormone concentration (C), inserts
4 factors of immersion treatment time (D) of fringe in hormone, 3 levels of each factor design are specifically shown in the following table 2.
Table 2- test process factor and level
Note: K1, K2, K3The average value of each factor rooting rate under different level is respectively indicated, letter indicates significant difference ratio
Compared with (following table is same).
Test 2 uses L9(34) Orthogonal Experiment and Design, 3 repetitions, overall design test such as table 3.
Table 3-L9(34) orthogonal test designs table
The result for testing the combination of 2 different disposals is as shown in table 4 below.
Table 4- different disposal combines the influence to FRUCTUS CITRI SARCODACTYLIS cuttage root-taking
Orthogonal test is carried out with orthogonal arrage according to the factor that table 2 designs, the results are shown in Table 4:
The FRUCTUS CITRI SARCODACTYLIS rooting rate highest of processing 4, reaches 98.9%, the quantity that nursery stock takes root with this condition is most, reaches
13.23, root long longest reaches 5.42cm；Wherein rooting rate processing 4 and processing 6, processing 3, processing 2 are without significant difference, with it
It, which is handled, reaches significant difference；Quantity of taking root and root long, processing 4 reach significant difference with other processing.
The FRUCTUS CITRI SARCODACTYLIS rooting rate of processing 7 is minimum, and only 65.56%, radical is also at least only 1.79.
Thus the optimised process group obtained is combined into the A of processing 42B1C2D3。
Each factor is as shown in table 5 below to the variance of FRUCTUS CITRI SARCODACTYLIS cutting plantation in test 2:
Variance analysis of each factor of table 5- to FRUCTUS CITRI SARCODACTYLIS cutting plantation
Shown in table 5 as above, the size of very poor R reflects the size that each factor influences survival rate, and very poor value is bigger, indicates
The factor influence factor is bigger.The very poor value of the rooting rate of 4 factors is followed successively by A > D > C > B, shows cutting medium to rooting rate
Influence it is maximum, nursery soil: coco bran=1:1 cutting medium rooting rate highest reaches 94.82%；Followed by soil reaches
90%；Nursery soil cutting plantation minimum 71.47%, and mutually reach significant difference level between three；When immersion treatment
Between on rooting rate influence take second place, wherein best with immersion treatment 30min；It is HORMONE TREATMENT concentration again, and hormone kind influences
It is minimum.
The selection of cuttings, the processing of cuttings and skewer are passed through using method provided by the invention it can be seen from statistical form
The selection for inserting matrix promotes to improve rooting percent, root long, root thickness；Then promoted by controlling temperature, the humidity of cuttage environment
Cuttings is sprouted reproduction speed and is accelerated, and then shortens growing-seedling period.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With.It can be applied to various suitable the field of the invention completely.It for those skilled in the art, can be easily
Realize other modification.
Claims (7)
1. a kind of method of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture, wherein include:
The cuttings for selecting semi-lignified, using NAA 200mg/L solution to cuttings immersion treatment 15 minutes, then by cuttings cuttage
Into the cutting medium prepared by nursery soil and coco bran by weight=1:1, finally carry out managing after cuttage can be used
In the FRUCTUS CITRI SARCODACTYLIS seedling of transplanting.
2. the method for FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture as described in claim 1, wherein after the cuttage management include:
After cuttings cuttage to cutting medium, control shading rate be 75%, control air themperature be 25 DEG C, air humidity be 40~
60%；In cuttage 40 days, control cutting medium humidity is 70~75%；
After cuttage 40 days, control cutting medium humidity is 55~65%, 80% or more air humidity, and keeps ventilated, skewer
The FRUCTUS CITRI SARCODACTYLIS seedling that can be used for transplanting is obtained after inserting 120 days.
3. the method for FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture as claimed in claim 2, wherein the selection of the cuttings specifically:
Take growth cycle FRUCTUS CITRI SARCODACTYLIS current year sprouting growth 6~10 months 3~8 years 1 centimetre of diameter or more to semi-lignified strong
Gained branch, is cut into 14~16 centimetres of fringe bar, removes fringe bar leaf and obtain the cuttings by the branch of strong no disease and pests harm, is sheared
Mouth needs to keep smooth.
4. the method for FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture as claimed in claim 3, wherein after removing fringe bar leaf, it is also necessary to use matter
The potassium permanganate for measuring score 0.5% carries out spray disinfectant to fringe bar, then rinses fringe bar water well and just obtains the cuttings.
5. the method for FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture as claimed in claim 4, wherein soaked using NAA 200mg/L solution to cuttings
Bubble is clean spare by cuttings washing after 15 minutes.
6. the method for FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture as claimed in claim 5, wherein the cutting medium the preparation method comprises the following steps:
It carries out nursery soil and coco bran to be mixed to get cutting medium with weight ratio=1:1, then to the cutting medium spraying concentration
10% carbendazim solution carries out disinfection, and spray suitable water so that the water content of the cutting medium reach 40~
60%.
7. the method for FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture as claimed in claim 6, wherein the nursery soil is turfy soil, vermiculite and treasure
Zhu Yan is mixed to get with the mass ratio of 4:1:1.
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| CN202210002106.XA CN114303778A (en) | 2019-06-27 | 2019-06-27 | Method for cutting and seedling of citrus bergamot |
| CN201910568209.0A CN110122087A (en) | 2019-06-27 | 2019-06-27 | The method of FRUCTUS CITRI SARCODACTYLIS cuttage and seedling culture |
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| CN114303778A (en) | 2022-04-12 |
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Application publication date: 20190816 |