CN107852971B - Method for storing sarcandra glabra seeds - Google Patents

Method for storing sarcandra glabra seeds Download PDF

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CN107852971B
CN107852971B CN201711050682.7A CN201711050682A CN107852971B CN 107852971 B CN107852971 B CN 107852971B CN 201711050682 A CN201711050682 A CN 201711050682A CN 107852971 B CN107852971 B CN 107852971B
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sarcandra glabra
sawdust
seeds
sarcandra
river sand
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CN107852971A (en
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璧靛钩
赵平
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Guilin Baolin Biotechnology Co ltd
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Rongan Jinyilin Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
    • A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of seed treatment, in particular to a method for storing sarcandra glabra seeds, which comprises the following steps: sterilizing sawdust and fine river sand, sterilizing sarcandra glabra fruit, mixing sterilized sawdust to perform embryo after-ripening, and mixing sterilized fine river sand to perform germination acceleration. According to the invention, during the sterilization treatment of the sarcandra glabra fruit, the sterilization liquid is adjusted to be slightly acidic, and meanwhile, the peel and pulp outside the sarcandra glabra seed are retained, so that the nutrient supply during the development of the seed embryo is ensured, and the slightly acidic condition can further promote the development of the seed embryo of the sarcandra glabra seed. In addition, the invention carries out sterilization treatment on the sawdust and the fine river sand, effectively avoids the molding of the sarcandra glabra seeds in the treatment process, and improves the germination rate and the health rate of the sarcandra glabra seeds.

Description

Method for storing sarcandra glabra seeds
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of seed treatment, in particular to a method for storing sarcandra glabra seeds.
[ background of the invention ]
Sarcandra glabra is perennial evergreen shrub of chloranthaceae, and has the following academic name: sarcandra glabra (Thunb.) Nakai, named as glabrous Sarcandra herb, Sarcandra glabra, sambucus williamsii, and the like, has beautiful shape and fragrance of four seasons, and has extremely high medicinal, edible and ornamental values. It has effects of clearing heat and cooling blood, promoting blood circulation and removing speckle, dispelling pathogenic wind and dredging collaterals, and can be used for treating blood heat, speckle and eruption, rheumatalgia, traumatic injury, etc. Modern pharmacological studies show that sarcandra glabra has multiple activities of resisting bacteria, diminishing inflammation, resisting tumors, improving immunity, promoting bone healing, relieving pain and asthma and the like. The herba Pileae Scriptae contains effective components such as metribuzin, isofraxidin, rosmarinic acid, isovanillic acid, curzerene, elemene, etc.
At present, the sarcandra glabra seeds have fewer resources and are in short supply and demand in the market, and the germination rate and emergence rate of the sarcandra glabra seeds are mainly improved, so that the yield and income of sarcandra glabra planting are improved. The sarcandra glabra seed seedling culture and cultivation are the main propagation mode of sarcandra glabra, and because the sarcandra glabra seeds harvested in autumn and winter almost have no seed embryo, the sarcandra glabra seeds can germinate after the embryo is mature. In the prior art, Wegyangweng and the like (influence of different storage modes on the germination of sarcandra glabra seeds, science of Guangxi forestry, 2016 (04) th, 462-; the second method is to preserve the pulp of the sarcandra glabra seeds and then perform embryo after-ripening, but the pulp is preserved, so that the sarcandra glabra seeds are mildewed and rotten in the embryo after-ripening process, and the health rate and the germination rate of the sarcandra glabra seeds are reduced.
[ summary of the invention ]
The invention aims to: aiming at the problems of insufficient nutrient supply and easy mildew generation in the treatment process of the sarcandra glabra seeds, the storage method of the sarcandra glabra seeds is high in germination rate, high in health rate, good in seed embryo development and difficult to mildew.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method for storing sarcandra glabra seeds comprises the following steps:
s1, taking sarcandra glabra fruits, sawdust and fine river sand, and sterilizing the sawdust and the fine river sand to obtain sterilized sawdust and sterilized fine river sand, wherein the sarcandra glabra fruits are picked between 12 middle ten days and 1 last ten days of the month, and the weight ratio of the sarcandra glabra fruits to the sawdust to the fine river sand is 100: 15-20: 230-300;
s2, sterilizing the sarcandra glabra fruits, and adjusting the pH value to 5.5-6.5 to obtain sterilized sarcandra glabra fruits;
s3, mixing the bactericidal sarcandra glabra fruits with the sterilized sawdust, uniformly mixing to obtain a sarcandra glabra fruit mixture, adjusting the relative humidity of the sarcandra glabra fruit mixture to be 75-85% by using sterile water, then transferring the sarcandra glabra fruit mixture into a storage room with the temperature of 3-10 ℃ to be placed in a dark place for 35-60 days, and keeping the water content of the sarcandra glabra fruit mixture to be 75-85% during the storage and placement period to obtain the sarcandra glabra fruit mixture;
s4, mixing the sarcandra glabra fruit mixture with the sterilized fine river sand, uniformly mixing, transferring into a germination accelerating greenhouse for standing for 15-20 days, and keeping the temperature in the germination accelerating greenhouse at 18-26 ℃ and the relative humidity at 75-85% during standing to obtain the dewwhite sarcandra glabra seeds.
Further, in step S1, the method of the sterilization process is as follows: carrying out damp-heat sterilization on the sawdust and the fine river sand, wherein the conditions of the damp-heat sterilization are as follows: maintaining the pressure at 103.4-137.3kPa and the temperature at 121.3-126.0 ℃ for 20-30 minutes, taking out, putting into a dry heat sterilization box, and performing dry heat sterilization under the conditions of: the temperature is 155-165 ℃, the time is 1.5-2.5 hours, and the product is taken out.
Further, in step S2, the method of the sterilization process is as follows: preparing a sterilizing solution, wherein the sterilizing solution comprises the following components, by mass, 10-12ppm of diethylaminoethanol caproate, 300-400ppm of carbendazim, 50-70ppm of carboxymethyl chitosan, 25-35ppm of brassinolide, 25-35ppm of 6-benzyl adenine and 0.3-0.7ppm of triacontanol, and the pH value is adjusted to be 5.5-6.5; adding the sarcandra glabra fruit into the sterilizing solution, soaking for 1-2 hours, and taking out.
Further, in step S3, the ratio of the sterilized sawdust to the sterilized sarcandra glabra fruit is 15-20 parts by weight: 100.
further, in step S4, the ratio of the sterilized fine river sand to the sarcandra glabra fruit mixture is 2-2.5: 1.
in summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the sarcandra glabra fruit sterilizing liquid is adopted to sterilize sarcandra glabra fruits, and sawdust and fine river sand are sterilized, so that the problems that the sarcandra glabra seeds are easy to mildew, the sarcandra glabra seeds are not fully after-ripened and the germination rate is low when the sarcandra glabra seeds with pulp are stored in the prior art are solved, and the sarcandra glabra seeds can draw the nutrients in the sarcandra glabra pulp to be fully after-ripened. The sarcandra glabra seeds stored by the method have the embryo rate of more than 98 percent, the health rate of more than 97.5 percent and the germination rate of more than 95.5 percent, and compared with the prior art, the sarcandra glabra seeds have multiple indexes which are obviously improved.
2. In the prior art, carbendazim is mostly adopted as a bactericide for sarcandra glabra seeds, but a single bactericide is easy to generate resistance after long-time use, and the bactericidal effect is poor. The invention adopts the combined use of the diethylaminoethanol caproate, the carboxymethyl chitosan, the carbendazim, the brassinolide, the 6-benzyladenine and the triacontanol, wherein the carboxymethyl chitosan has stronger antibacterial effect and can enhance the continuous antibacterial ability of the sarcandra glabra seeds; brassinolide, 6-benzyladenine and triacontanol have the functions of promoting cell division and stimulating the growth of plant trophosome, further promote the after-ripening of embryos and improve the embryo rate of sarcandra glabra seeds. On the other hand, the sarcandra glabra seeds are suitable for performing embryo after-ripening under the slightly acidic condition, but the step of adjusting the pH value of the sarcandra glabra seed sterilizing solution is lacked in the prior art, the pH value of the sterilizing solution is adjusted to be slightly acidic, the effects of further promoting the embryo after-ripening of the sarcandra glabra seeds and improving the health rate and germination rate of the seeds can be further promoted, and meanwhile, the emergence rate of the sarcandra glabra is improved, and the pest and disease damage conditions of the sarcandra glabra in the seedling stage are reduced. Compared with the sarcandra glabra seeds obtained by not adopting seed soaking treatment, the sarcandra glabra seeds obtained by the method have the advantages that the embryo rate is improved by 27.91%, and the health rate is improved by 31.50%.
3. In the prior art, when the sarcandra glabra seeds are treated by a method without removing pulp, the sarcandra glabra seeds are easy to mildew in the process of embryo after-ripening, the embryo after-ripening and the germination process of the seeds are influenced, and the germination rate and the health rate of the sarcandra glabra seeds are reduced. According to the invention, in the process of after-ripening of the sarcandra glabra seed embryo, the pulp part of the sarcandra glabra seed is reserved, the nutrient supply in the after-ripening process of the sarcandra glabra seed embryo is improved, the sarcandra glabra seed can absorb the nutrients in the pulp, and meanwhile, aiming at the problem that the embryo is easy to mildew in the after-ripening and germination processes, the invention also performs sterilization treatment on sawdust and fine river sand used in the treatment process of the sarcandra glabra seed embryo, so that the possibility of mildew in the after-ripening process of the sarcandra glabra seed embryo is greatly reduced; the sterilized sawdust also has good moisturizing effect, so that water supply in the process of after-ripening of sarcandra glabra seed embryos is guaranteed, and the seeds are mixed with sterilized fine river sand after the seed embryos are matured, so that the pulp of the sarcandra glabra seeds is destroyed, the destruction of the pulp is accelerated, the sarcandra glabra seeds are whitened as soon as possible, and the sarcandra glabra seed can be used for seedling culture. Compared with the sarcandra glabra seeds stored by adopting non-sterilized sawdust and fine river sand, the health rate of the sarcandra glabra seeds stored by the method is improved by more than 45 percent.
[ detailed description ] embodiments
The present invention will be further described with reference to the following examples.
Example 1
S1, taking sarcandra glabra fruits, sawdust and fine river sand, and sterilizing the sawdust and the fine river sand to obtain sterilized sawdust and sterilized fine river sand, wherein the sarcandra glabra fruits are picked between 12 middle ten days and 1 last ten days of the month, and the weight ratio of the sarcandra glabra fruits to the sawdust to the fine river sand is 100: 20: 300, respectively;
s2, sterilizing the sarcandra glabra fruits, and adjusting the pH value to 5.5 to obtain sterilized sarcandra glabra fruits;
s3, mixing the bactericidal sarcandra glabra fruits with the sterilized sawdust, uniformly mixing to obtain a sarcandra glabra fruit mixture, adjusting the relative humidity of the sarcandra glabra fruit mixture to be 85% by using sterile water, then transferring the sarcandra glabra fruit mixture into a storage room with the temperature of 10 ℃ to be placed in a dark place for 35 days, and keeping the water content of the sarcandra glabra fruit mixture to be 85% during the storage and placement period to obtain the sarcandra glabra fruit mixture;
and S4, mixing the sarcandra glabra fruit mixture with the sterilized fine river sand, uniformly mixing, transferring into a germination accelerating greenhouse for standing for 15 days, and keeping the temperature in the germination accelerating greenhouse at 26 ℃ and the relative humidity at 85% during standing to obtain the sarcandra glabra seeds.
In step S1 of the present embodiment, the method of the sterilization process is as follows: carrying out damp-heat sterilization on the sawdust and the fine river sand, wherein the conditions of the damp-heat sterilization are as follows: maintaining the pressure at 103.4kPa and the temperature at 121.3 ℃ for 30 minutes, taking out, putting into a dry heat sterilization box, and performing dry heat sterilization, wherein the dry heat sterilization conditions are as follows: the temperature was 155 ℃ and the time was 2.5 hours, and the sample was taken out.
In step S2 of this embodiment, the method of the sterilization process is as follows: preparing a sterilizing solution, wherein the sterilizing solution comprises the following components in mass concentration, 12ppm of diethylaminoethanol caproate, 400ppm of carbendazim, 70ppm of carboxymethyl chitosan, 35ppm of brassinolide, 35ppm of 6-benzyladenine and 0.7ppm of triacontanol, and the pH value is adjusted to be 5.5; adding the sarcandra glabra fruit into the sterilizing solution, soaking for 1 hour, and taking out.
Example 2
S1, taking sarcandra glabra fruits, sawdust and fine river sand, and sterilizing the sawdust and the fine river sand to obtain sterilized sawdust and sterilized fine river sand, wherein the sarcandra glabra fruits are picked between 12 middle ten days and 1 last ten days of the month, and the weight ratio of the sarcandra glabra fruits to the sawdust to the fine river sand is 100: 15: 230;
s2, sterilizing the sarcandra glabra fruits, and adjusting the pH value to 6.5 to obtain sterilized sarcandra glabra fruits;
s3, mixing the bactericidal sarcandra glabra fruits with the sterilized sawdust, uniformly mixing to obtain a sarcandra glabra fruit mixture, adjusting the relative humidity of the sarcandra glabra fruit mixture to be 75% by using sterile water, then transferring the sarcandra glabra fruit mixture into a storage room with the temperature of 3 ℃ to be placed in a dark place for 60 days, and keeping the water content of the sarcandra glabra fruit mixture to be 75% during the storage and placement period to obtain the sarcandra glabra fruit mixture;
and S4, mixing the sarcandra glabra fruit mixture with the sterilized fine river sand, uniformly mixing, transferring into a germination accelerating greenhouse, standing for 20 days, and keeping the temperature in the germination accelerating greenhouse at 18 ℃ and the relative humidity at 75% during standing to obtain the dewwhite sarcandra glabra seeds.
In step S1 of the present embodiment, the method of the sterilization process is as follows: carrying out damp-heat sterilization on the sawdust and the fine river sand, wherein the conditions of the damp-heat sterilization are as follows: maintaining the pressure at 137.3kPa and the temperature at 126.0 ℃ for 20 minutes, taking out, putting into a dry heat sterilization box, and performing dry heat sterilization under the conditions that: the temperature was 165 ℃ and the time was 1.5 hours, and the sample was taken out.
In step S2 of this embodiment, the method of the sterilization process is as follows: preparing a sterilizing solution, wherein the sterilizing solution comprises the following components, by mass, 10ppm of diethylaminoethanol caproate, 300ppm of carbendazim, 50ppm of carboxymethyl chitosan, 25ppm of brassinolide, 25ppm of 6-benzyladenine and 0.3ppm of triacontanol, and the pH value is adjusted to be 6.5; adding the sarcandra glabra fruit into the sterilizing solution, soaking for 2 hours, and taking out.
Example 3
S1, taking sarcandra glabra fruits, sawdust and fine river sand, and sterilizing the sawdust and the fine river sand to obtain sterilized sawdust and sterilized fine river sand, wherein the sarcandra glabra fruits are picked between 12 middle ten days and 1 last ten days of the month, and the weight ratio of the sarcandra glabra fruits to the sawdust to the fine river sand is 100: 16: 245;
s2, sterilizing the sarcandra glabra fruits, and adjusting the pH value to 5.7 to obtain sterilized sarcandra glabra fruits;
s3, mixing the bactericidal sarcandra glabra fruits with the sterilized sawdust, uniformly mixing to obtain a sarcandra glabra fruit mixture, adjusting the relative humidity of the sarcandra glabra fruit mixture to be 77% by using sterile water, then transferring the sarcandra glabra fruit mixture into a storage room with the temperature of 5 ℃ to be placed in a dark place for 55 days, and keeping the water content of the sarcandra glabra fruit mixture to be 77% during the storage and placement period to obtain the sarcandra glabra fruit mixture;
and S4, mixing the sarcandra glabra fruit mixture with the sterilized fine river sand, uniformly mixing, transferring into a germination accelerating greenhouse, standing for 16 days, and keeping the temperature in the germination accelerating greenhouse at 19 ℃ and the relative humidity at 77% during standing to obtain the dewwhite sarcandra glabra seeds.
In step S1 of the present embodiment, the method of the sterilization process is as follows: carrying out damp-heat sterilization on the sawdust and the fine river sand, wherein the conditions of the damp-heat sterilization are as follows: maintaining the pressure at 109.9kPa and the temperature at 122.0 ℃ for 28 minutes, taking out, putting into a dry heat sterilization box, and performing dry heat sterilization, wherein the dry heat sterilization conditions are as follows: the temperature was 157 ℃ and the time was 1.8 hours, and the reaction mixture was taken out.
In step S2 of this embodiment, the method of the sterilization process is as follows: preparing a sterilizing solution, wherein the sterilizing solution comprises the following components, by mass, 11ppm of diethylaminoethanol caproate, 320ppm of carbendazim, 54ppm of carboxymethyl chitosan, 27ppm of brassinolide, 27ppm of 6-benzyladenine and 0.4ppm of triacontanol, and the pH value is adjusted to be 5.7; adding the sarcandra glabra fruit into the sterilizing solution, soaking for 1.2 hours, and taking out.
Example 4
S1, taking sarcandra glabra fruits, sawdust and fine river sand, and sterilizing the sawdust and the fine river sand to obtain sterilized sawdust and sterilized fine river sand, wherein the sarcandra glabra fruits are picked between 12 middle ten days and 1 last ten days of the month, and the weight ratio of the sarcandra glabra fruits to the sawdust to the fine river sand is 100: 17: 265 of a nitrogen-containing gas;
s2, sterilizing the sarcandra glabra fruits, and adjusting the pH value to 6 to obtain sterilized sarcandra glabra fruits;
s3, mixing the bactericidal sarcandra glabra fruits with the sterilized sawdust, uniformly mixing to obtain a sarcandra glabra fruit mixture, adjusting the relative humidity of the sarcandra glabra fruit mixture to 80% by using sterile water, then transferring the sarcandra glabra fruit mixture into a storage room with the temperature of 6 ℃ to be placed in a dark place for 50 days, and keeping the water content of the sarcandra glabra fruit mixture to be 80% during the storage and placement period to obtain the sarcandra glabra fruit mixture;
and S4, mixing the sarcandra glabra fruit mixture with the sterilized fine river sand, uniformly mixing, transferring into a germination accelerating greenhouse, standing for 17 days, and keeping the temperature in the germination accelerating greenhouse at 22 ℃ and the relative humidity at 80% during standing to obtain the dewwhite sarcandra glabra seeds.
In step S1 of the present embodiment, the method of the sterilization process is as follows: carrying out damp-heat sterilization on the sawdust and the fine river sand, wherein the conditions of the damp-heat sterilization are as follows: maintaining the pressure at 123.5kPa and the temperature at 124.0 ℃ for 25 minutes, taking out, putting into a dry heat sterilization box, and performing dry heat sterilization under the conditions that: the temperature is 160 ℃, the time is 2 hours, and the product is taken out.
In step S2 of this embodiment, the method of the sterilization process is as follows: preparing a sterilizing solution, wherein the sterilizing solution comprises the following components, by mass, 11ppm of diethylaminoethanol caproate, 350ppm of carbendazim, 60ppm of carboxymethyl chitosan, 30ppm of brassinolide, 30ppm of 6-benzyladenine and 0.5ppm of triacontanol, and the pH value is adjusted to be 6; adding the sarcandra glabra fruit into the sterilizing solution, soaking for 1.5 hours, and taking out.
Example 5
S1, taking sarcandra glabra fruits, sawdust and fine river sand, and sterilizing the sawdust and the fine river sand to obtain sterilized sawdust and sterilized fine river sand, wherein the sarcandra glabra fruits are picked between 12 middle ten days and 1 last ten days of the month, and the weight ratio of the sarcandra glabra fruits to the sawdust to the fine river sand is 100: 19: 280 parts of;
s2, sterilizing the sarcandra glabra fruits, and adjusting the pH value to 6.3 to obtain sterilized sarcandra glabra fruits;
s3, mixing the bactericidal sarcandra glabra fruits with the sterilized sawdust, uniformly mixing to obtain a sarcandra glabra fruit mixture, adjusting the relative humidity of the sarcandra glabra fruit mixture to 83% by using sterile water, then transferring the sarcandra glabra fruit mixture into a storage room with the temperature of 8 ℃ to be placed in a dark place for 45 days, and keeping the water content of the sarcandra glabra fruit mixture to be 83% during the storage and placement period to obtain the sarcandra glabra fruit mixture;
and S4, mixing the sarcandra glabra fruit mixture with the sterilized fine river sand, uniformly mixing, transferring into a germination accelerating greenhouse, standing for 19 days, and keeping the temperature in the germination accelerating greenhouse at 24 ℃ and the relative humidity at 83% during the standing period to obtain the dewwhite sarcandra glabra seeds.
In step S1 of the present embodiment, the method of the sterilization process is as follows: carrying out damp-heat sterilization on the sawdust and the fine river sand, wherein the conditions of the damp-heat sterilization are as follows: maintaining the pressure at 130.5kPa and the temperature at 125.0 ℃ for 22 minutes, taking out, putting into a dry heat sterilization box, and performing dry heat sterilization, wherein the dry heat sterilization conditions are as follows: the temperature was 163 ℃ and the time was 2.2 hours, and the sample was taken out.
In step S2 of this embodiment, the method of the sterilization process is as follows: preparing a sterilizing solution, wherein the sterilizing solution comprises the following components in mass concentration, 12ppm of diethylaminoethanol caproate, 380ppm of carbendazim, 68ppm of carboxymethyl chitosan, 33ppm of brassinolide, 33ppm of 6-benzyladenine and 0.6ppm of triacontanol, and the pH value is adjusted to be 6.3; adding the sarcandra glabra fruit into the sterilizing solution, soaking for 1.8 hours, and taking out.
Example 6
The same sarcandra glabra seed storage method as in example 1 was used, except that the harvested sarcandra glabra fruits were immediately peeled to remove the flesh and only the sarcandra glabra seeds were remained.
Example 7
The same sarcandra glabra seed storage method as in example 1 was used except that the sawdust and fine river sand were not subjected to sterilization treatment.
Example 8
The same sarcandra glabra seed storage method as in example 1 was used except that the sarcandra glabra fruit was not sterilized with the sterilizing liquid.
Example 9
The sarcandra glabra seeds obtained in examples 1 to 5 were designated as samples 1 to 5, and the sarcandra glabra seeds obtained in examples 6 to 8 were designated as comparative samples 1 to 3.
The embryo rate was determined by randomly selecting 30 sarcandra glabra fruits treated in step S3 in each example, removing the peel and pulp to obtain sarcandra glabra seeds, splitting the seeds vertically, measuring the length of the embryo and the length of the endosperm with a vernier caliper, and calculating the embryo rate at × 100% embryo rate (embryo length/endosperm length).
The health rate was determined by randomly selecting 100 of the lucerne clearweed seeds treated in step S4 in each example, wherein healthy seeds and rotten and moldy unhealthy seeds were filled and hard, repeating the steps 2 times, and calculating the health rate as × 100% of the health rate (healthy seeds/total number of seeds measured).
The method for measuring the germination rate comprises the steps of randomly taking 100 lucerne sarcandra glabra seeds treated in the step S4, placing the seeds in a culture dish and a double-filter paper bed, culturing the seeds at the constant temperature of 25 ℃ for 20 days, repeating the culturing for 2 times, and calculating the germination rate according to the germination rate (total number of germinated seeds/measured seeds) of × 100%.
The method for measuring the emergence rate comprises the following steps: randomly picking 300 sarcandra glabra seeds processed in the step S4, transplanting the sarcandra glabra seeds into a seedling raising greenhouse for raising seedlings, sowing for 4 months and 1 day till the emergence of seedlings is finished in 4 months and 28 days, counting seedling emergence results, and counting seedling-stage pest and disease damage conditions of the sarcandra glabra after emergence, wherein the seedling-stage pest and disease damage conditions are mainly the conditions of black mold and anthracnose.
The results of the measurement of the sarcandra glabra seeds treated in each example are shown in Table 1.
TABLE 1 sarcandra glabra seed assay results
Figure BDA0001453111440000091
Figure BDA0001453111440000101
As can be seen from Table 1, the sarcandra glabra seeds treated by the method of the present invention, samples 1-5, exhibited well-developed embryos that were substantially completely developed. In example 6 in which the sarcandra glabra peel and pulp are removed, the embryo rate of the sarcandra glabra seed is reduced by 34.87% compared with that of sample 1 because the sarcandra glabra seed lacks the nutrients supplied by the pulp; compared with the example 8 adopting non-sterilized sawdust and fine river sand, the health rate of the sample 1 is reduced by 47% by finding that more sarcandra glabra seeds in the comparative sample 2 are mildewed, which is presumed to be caused by microorganisms brought by sawdust and river sand, while the sarcandra glabra seeds treated by the examples 1-5 of the invention have extremely high health rate as a whole; in example 8, since the sarcandra glabra fruits are not sterilized by the sterilization liquid, compared with sample 1 in example 1, the embryo rate of comparative sample 3 is reduced by 25.98%, the health rate is reduced by 31.50%, the germination rate is reduced by 24.00%, in the seedling raising process, the seedling emergence rate is reduced by 25.33%, and more diseases and insect pests of black mold and anthracnose appear in the seedling stage, but the sarcandra glabra seeds stored by the invention have few diseases and insect pests in the seedling stage, and the comprehensive disease and insect pest rate is less than 1.5%. Therefore, the sarcandra glabra seeds treated by the method have the characteristics of good embryo development, less mildew, high health rate and germination rate, and meanwhile, the emergence rate of the sarcandra glabra seeds in a seedling stage is improved, and the disease and insect damage conditions of the sarcandra glabra seeds in the seedling stage are reduced.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.

Claims (5)

1. The method for storing the sarcandra glabra seeds is characterized by comprising the following steps of:
s1, taking sarcandra glabra fruits, sawdust and fine river sand, and sterilizing the sawdust and the fine river sand to obtain sterilized sawdust and sterilized fine river sand, wherein the sarcandra glabra fruits are picked between 12 middle ten days and 1 last ten days of the month, and the weight ratio of the sarcandra glabra fruits to the sawdust to the fine river sand is 100: 15-20: 230-300;
s2, sterilizing the sarcandra glabra fruits, wherein the sterilizing method comprises the following steps: preparing a sterilizing solution, wherein the sterilizing solution comprises the following components, by mass, 10-12ppm of diethylaminoethanol caproate, 300-400ppm of carbendazim, 50-70ppm of carboxymethyl chitosan, 25-35ppm of brassinolide, 25-35ppm of 6-benzyl adenine and 0.3-0.7ppm of triacontanol, and the pH value is adjusted to be 5.5-6.5; adding the sarcandra glabra fruit into the sterilizing solution, soaking for 1-2 hours, and taking out to obtain the sarcandra glabra fruit;
s3, mixing the bactericidal sarcandra glabra fruits with the sterilized sawdust, uniformly mixing to obtain a sarcandra glabra fruit mixture, adjusting the relative humidity of the sarcandra glabra fruit mixture to be 75-85% by using sterile water, then transferring the sarcandra glabra fruit mixture into a storage room with the temperature of 3-10 ℃ to be placed in a dark place for 35-60 days, and keeping the water content of the sarcandra glabra fruit mixture to be 75-85% during the storage and placement period to obtain the sarcandra glabra fruit mixture;
s4, mixing the sarcandra glabra fruit mixture with the sterilized fine river sand, uniformly mixing, transferring into a germination accelerating greenhouse for standing for 15-20 days, and keeping the temperature in the germination accelerating greenhouse at 18-26 ℃ and the relative humidity at 75-85% during standing to obtain the dewwhite sarcandra glabra seeds.
2. The method for storing sarcandra glabra seeds as claimed in claim 1, wherein the sterilization treatment in step S1 is performed by: carrying out damp-heat sterilization on the sawdust and the fine river sand, wherein the conditions of the damp-heat sterilization are as follows: maintaining the pressure at 103.4-137.3kPa and the temperature at 121.3-126.0 ℃ for 20-30 minutes, taking out, putting into a dry heat sterilization box, and performing dry heat sterilization under the conditions of: the temperature is 155-165 ℃, the time is 1.5-2.5 hours, and the product is taken out.
3. The method of claim 1, wherein in step S3, the ratio of the sterilized sawdust to the sterilized sarcandra glabra fruit is 15-20 parts by weight: 100.
4. the method of claim 1, wherein in step S4, the ratio of the sterilized fine river sand to the mixture of sarcandra glabra fruits is 2-2.5: 1.
5. a sarcandra glabra seed obtained by the method for storing sarcandra glabra seeds of any one of claims 1 to 4.
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