CN107852971A - A kind of storage method of Chloranthus glaber seed - Google Patents

A kind of storage method of Chloranthus glaber seed Download PDF

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Publication number
CN107852971A
CN107852971A CN201711050682.7A CN201711050682A CN107852971A CN 107852971 A CN107852971 A CN 107852971A CN 201711050682 A CN201711050682 A CN 201711050682A CN 107852971 A CN107852971 A CN 107852971A
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chloranthus glaber
fruit
seed
river sand
sterilization
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CN201711050682.7A
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CN107852971B (en
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璧靛钩
赵平
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GUILIN BAOLIN BIOTECHNOLOGY Co.,Ltd.
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Rongan County Gold Biotechnology Suitable For Afforestation Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01FPROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
    • A01F25/00Storing agricultural or horticultural produce; Hanging-up harvested fruit
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02Acyclic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring

Abstract

The present invention relates to seed processing technology field, and in particular to a kind of storage method of Chloranthus glaber seed, this method comprise the following steps:Sterilization treatment is carried out to wooden chaff and thin river sand, sterilization processing is carried out to Chloranthus glaber fruit, the wooden chaff of sterilizing is admixed and carries out embryo after-ripening processing, admixes the thin river sand of sterilizing and carry out vernalization processing.For the present invention in Chloranthus glaber fruit sterilization processing, regulation bactericidal liquid is subacidity, while remains the extraseminal pericarp of Chloranthus glaber and pulp, ensure that nutrient supply during embryo development, and slightly acidic condition can further promote the development of Chloranthus glaber seed embryo.The present invention has carried out sterilization treatment to wooden chaff and thin river sand in addition, effectively prevent and is gone mouldy in Chloranthus glaber seed treatment processes, improves the germination rate of Chloranthus glaber seed and healthy rate.

Description

A kind of storage method of Chloranthus glaber seed
【Technical field】
The present invention relates to seed processing technology field, and in particular to a kind of storage method of Chloranthus glaber seed.
【Background technology】
Chloranthus glaber is Chloranthaceae perennial evergreen undershrub, scientific name:Sarcandra glabra (Thunb.) Nakai, Also known as Glabrous Sarcandra Herb, sarcandra, elder etc., form is beautiful, four seasons fragrance, has high medicinal, edible and ornamental value. 《Chinese Pharmacopoeia》Described in its have clearing heat and cooling blood, promoting blood circulation ecchymose removing, dispelling wind and removing obstruction in the meridians the effect of, for blood-head spot dermexanthesis, rheumatism The diseases such as numbness pain, traumatic injury.Modern pharmacology research shows that Chloranthus glaber has anti-inflammation, antitumor, raising is immune, promotes bone The various actives such as bone is healed and analgesia is relievingd asthma.Contain piperazine skin element, isofraxidin, Rosmarinic acid, isovanillic acid, curcuma zedoary in Chloranthus glaber The active ingredients such as alkene, elemene.
At present, Chloranthus glaber seed resource is less, and supply falls short of demand in market, rely primarily on improve Chloranthus glaber seed germination percentage, Emergence rate, so as to improve the yield and income of Chloranthus glaber plantation.The cultivation of Chloranthus glaber seed seedling-raising is the main breeding side of Chloranthus glaber Formula, due to autumn and winter, the Chloranthus glaber seed of harvesting does not almost have embryo, it is necessary to could be germinateed by embryo after-ripening.Prior art In, (influence that different storage methods are sprouted to Chloranthus glaber seed, Guangxi forest-science, 04 phase in 2016, the 462- such as Wei Yingwen 464, ISSN:The storage method of two kinds of Chloranthus glaber seeds 1006-1126) is reported, first method is that Chloranthus glaber seed is entered Go and removed follicarpium meat, then carried out embryo after-ripening process, but due to eliminating pulp, Chloranthus glaber seed is supported during causing embryo after-ripening Point insufficient supply, embryo development it is insufficient;Second method is the pulp to reservation Chloranthus glaber seed, then carries out embryo after-ripening, But due to remaining pulp, it result in the Chloranthus glaber seed during embryo after-ripening and the phenomenon gone mouldy, rotted occur, reduce careless coral The healthy rate and germination rate of coral seed.
【The content of the invention】
The goal of the invention of the present invention is:For in Chloranthus glaber seed treatment processes nutrient supply deficiency, easily go mouldy The problem of, there is provided a kind of high germination rate, high healthy rate, embryo development is good, is not susceptible to the storage of Chloranthus glaber seed gone mouldy Method.
To achieve these goals, the technical solution adopted by the present invention is as follows:
A kind of storage method of Chloranthus glaber seed, comprises the following steps:
S1. Chloranthus glaber fruit, wooden chaff and thin river sand are taken, sterilization treatment is carried out to the wooden chaff and thin river sand, sterilized Wooden chaff and the thin river sand that sterilizes, the Chloranthus glaber fruit are plucked between mid-December-early January and obtained, the Chloranthus glaber fruit, The weight of wooden chaff and thin river sand ratio is 100:15-20:230-300;
S2. sterilization processing is carried out to the Chloranthus glaber fruit, and adjusts pH value to 5.5-6.5, obtain sterilizing Chloranthus glaber fruit It is real;
S3. the sterilization Chloranthus glaber fruit is admixed in the wooden chaff of sterilizing, is well mixed to obtain Chloranthus glaber fruit mixture, Relative humidity with sterilized water regulation coral fruit mixture is 75-85%, is then moved into the storeroom that temperature is 3-10 DEG C Avoid light place 35-60 days, storage keeps the water content of coral fruit mixture during placing be 75-85%, obtains Chloranthus glaber fruit Real mixture;
S4. the Chloranthus glaber fruit mixture is admixed in the thin river sand of sterilizing, is well mixed, moves into vernalization greenhouse Place 15-20 days, keep the temperature in vernalization greenhouse to be showed money or valuables one carries unintentionally in 18-26 DEG C, relative humidity 75-85% during placement Chloranthus glaber seed.
Further, in step S1, the method for the sterilization treatment is as follows:The wooden chaff and thin river sand are subjected to damp and hot go out Bacterium, wherein the condition of the moist heat sterilization is:Pressure is 103.4-137.3kPa, and temperature is 121.3-126.0 DEG C, maintains 20- 30 minutes, take out, be put into oven, carry out hot air sterilization, the condition of the hot air sterilization is:Temperature is 155-165 DEG C, Time is 1.5-2.5 hours, is taken out.
Further, in step S2, the method for the sterilization processing is as follows:Prepare germicidal solution, the germicidal solution bag Include the composition of following mass concentration, 10-12ppm diethylamino ethanol caproates, 300-400ppm carbendazim, 50-70ppm carboxylic first Base enclosure glycan, 25-35ppm brassinosteroids, 25-35ppm6- benzyladenines, 0.3-0.7ppm triacontanols, and adjust pH It is worth for 5.5-6.5;The Chloranthus glaber fruit is added into the germicidal solution, soaks 1-2 hours, is taken out.
Further, in step S3, by weight, the parts by weight of the wooden chaff of sterilizing and the sterilization Chloranthus glaber fruit Than for 15-20:100.
Further, in step S4, by weight, the weight of the thin river sand of sterilizing and the Chloranthus glaber fruit mixture Amount part is compared for 2-2.5:1.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows:
1. being sterilized present invention employs bactericidal liquid to Chloranthus glaber fruit, and wooden chaff and thin river sand are sterilized Processing, overcomes that prior art is easily gone mouldy in storage band pulp Chloranthus glaber seed, Chloranthus glaber seed afterripening is incomplete, sends out The problem of bud rate is low, the nutrient that realizing Chloranthus glaber seed can draw in Chloranthus glaber pulp carry out abundant after-ripening.Using this hair The Chloranthus glaber seed of bright method caching, the embryo rate of Chloranthus glaber seed can reach more than 98%, and healthy rate reaches more than 97.5%, Germination rate reaches more than 95.5%, and compared with the prior art, many index is obviously improved.
2. in the prior art, use bactericide of the carbendazim as Chloranthus glaber seed, but much time using is single kills more Microbial inoculum is also easy to produce resistance, and bactericidal effect is bad.Present invention employs diethylamino ethanol caproate, carboxymethyl chitosan, more bacterium Spirit, brassinosteroid, 6-benzyladenine, triacontanol combination, wherein carboxymethyl chitosan has stronger antibacterial effect, can To strengthen the sustained anti-microbial ability of Chloranthus glaber seed, diethylamino ethanol caproate and bactericide compounded use, have obvious Synergistic effect, seed resistance against diseases can be strengthened, improve bactericidal effect, while the dosage of bactericide can also be reduced, further Reduce cost and reduce the generation of microbial resistance;Brassinosteroid, 6-benzyladenine and triacontanol are respectively provided with rush The effect entered cell division and stimulate phytoma to grow, further promotes embryo after-ripening, improves the embryo rate of Chloranthus glaber seed. On the other hand, Chloranthus glaber seed suitably carries out embryo after-ripening under conditions of subacidity, and lacks regulation Chloranthus glaber in the prior art The pH value of bactericidal liquid is adjusted to subacidity, can further promote Chloranthus glaber kind by the step of seed bactericidal liquid pH value, the present invention The effect of the embryo after-ripening of son, the healthy rate for improving seed and germination rate, while also improve emergence rate and the reduction of Chloranthus glaber The pest and disease damage situation in Chloranthus glaber seedling stage.The inventive method handles obtained Chloranthus glaber seed, compared to not obtained using seed soaking The Chloranthus glaber seed arrived, embryo rate improve 27.91%, and healthy rate improves 31.50%.
3. in the prior art, handle Chloranthus glaber seed using the method for not removing pulp, Chloranthus glaber seed is in embryo after-ripening During easily go mouldy, influence the embryo after-ripening of seed and the process of sprouting, reduce Chloranthus glaber seed germination rate and Healthy rate.The present invention remains the pulp fraction of Chloranthus glaber seed, improves careless coral during Chloranthus glaber seed embryo after-ripening Nutrient supply in coral seed embryo After-ripening, Chloranthus glaber seed is drawn the nutrient in pulp, while be directed to embryo after-ripening And the problem of easily being gone mouldy in germination process, wooden chaff, thin river sand of the present invention also to being used in Chloranthus glaber seed treatment processes Sterilization treatment has been carried out, has greatly reduced the possibility gone mouldy in Chloranthus glaber seed embryo After-ripening;Wherein sterilize wooden chaff Also there is good moisture-keeping function, ensure that the moisture in Chloranthus glaber seed embryo After-ripening supplies, after embryo development maturation, then mix Enter the thin river sand that sterilizes, destroy the pulp of Chloranthus glaber seed and accelerate the destruction of pulp, Chloranthus glaber seed is showed money or valuables one carries unintentionally as early as possible, you can use In nursery.Compared with the Chloranthus glaber seed stored using non-sterile wooden chaff and thin river sand, the careless coral of the inventive method storage The healthy rate of coral seed improves more than 45%.
【Embodiment】
With reference to embodiment, the invention will be further described.
Embodiment 1
S1. Chloranthus glaber fruit, wooden chaff and thin river sand are taken, sterilization treatment is carried out to the wooden chaff and thin river sand, sterilized Wooden chaff and the thin river sand that sterilizes, the Chloranthus glaber fruit are plucked between mid-December-early January and obtained, the Chloranthus glaber fruit, The weight of wooden chaff and thin river sand ratio is 100:20:300;
S2. sterilization processing is carried out to the Chloranthus glaber fruit, and adjusts pH value to 5.5, obtain sterilizing Chloranthus glaber fruit;
S3. the sterilization Chloranthus glaber fruit is admixed in the wooden chaff of sterilizing, is well mixed to obtain Chloranthus glaber fruit mixture, Relative humidity with sterilized water regulation coral fruit mixture is 85%, then moves into lucifuge in the storeroom that temperature is 10 DEG C and puts Put 35 days, storage keeps the water content of coral fruit mixture during placing be 85%, obtains Chloranthus glaber fruit mixture;
S4. the Chloranthus glaber fruit mixture is admixed in the thin river sand of sterilizing, is well mixed, moves into vernalization greenhouse Place 15 days, keep the temperature in vernalization greenhouse to obtain the Chloranthus glaber kind that shows money or valuables one carries unintentionally in 26 DEG C, relative humidity 85% during placement Son.
In the present embodiment step S1, the method for the sterilization treatment is as follows:The wooden chaff and thin river sand are subjected to damp and hot go out Bacterium, wherein the condition of the moist heat sterilization is:Pressure is 103.4kPa, and temperature is 121.3 DEG C, maintains 30 minutes, takes out, be put into Oven, carries out hot air sterilization, and the condition of the hot air sterilization is:Temperature is 155 DEG C, and the time is 2.5 hours, is taken out.
In the present embodiment step S2, the method for the sterilization processing is as follows:Germicidal solution is prepared, the germicidal solution includes The composition of following mass concentration, 12ppm diethylamino ethanol caproates, 400ppm carbendazim, 70ppm carboxymethyl chitosans, 35ppm brassinosteroids, 35ppm6- benzyladenines, 0.7ppm triacontanols, and pH value is adjusted as 5.5;It is molten to the sterilization The Chloranthus glaber fruit is added in liquid, is soaked 1 hour, is taken out.
Embodiment 2
S1. Chloranthus glaber fruit, wooden chaff and thin river sand are taken, sterilization treatment is carried out to the wooden chaff and thin river sand, sterilized Wooden chaff and the thin river sand that sterilizes, the Chloranthus glaber fruit are plucked between mid-December-early January and obtained, the Chloranthus glaber fruit, The weight of wooden chaff and thin river sand ratio is 100:15:230;
S2. sterilization processing is carried out to the Chloranthus glaber fruit, and adjusts pH value to 6.5, obtain sterilizing Chloranthus glaber fruit;
S3. the sterilization Chloranthus glaber fruit is admixed in the wooden chaff of sterilizing, is well mixed to obtain Chloranthus glaber fruit mixture, Relative humidity with sterilized water regulation coral fruit mixture is 75%, then moves into lucifuge in the storeroom that temperature is 3 DEG C and puts Put 60 days, storage keeps the water content of coral fruit mixture during placing be 75%, obtains Chloranthus glaber fruit mixture;
S4. the Chloranthus glaber fruit mixture is admixed in the thin river sand of sterilizing, is well mixed, moves into vernalization greenhouse Place 20 days, keep the temperature in vernalization greenhouse to obtain the Chloranthus glaber kind that shows money or valuables one carries unintentionally in 18 DEG C, relative humidity 75% during placement Son.
In the present embodiment step S1, the method for the sterilization treatment is as follows:The wooden chaff and thin river sand are subjected to damp and hot go out Bacterium, wherein the condition of the moist heat sterilization is:Pressure is 137.3kPa, and temperature is 126.0 DEG C, maintains 20 minutes, takes out, be put into Oven, carries out hot air sterilization, and the condition of the hot air sterilization is:Temperature is 165 DEG C, and the time is 1.5 hours, is taken out.
In the present embodiment step S2, the method for the sterilization processing is as follows:Germicidal solution is prepared, the germicidal solution includes The composition of following mass concentration, 10ppm diethylamino ethanol caproates, 300ppm carbendazim, 50ppm carboxymethyl chitosans, 25ppm brassinosteroids, 25ppm6- benzyladenines, 0.3ppm triacontanols, and pH value is adjusted as 6.5;It is molten to the sterilization The Chloranthus glaber fruit is added in liquid, is soaked 2 hours, is taken out.
Embodiment 3
S1. Chloranthus glaber fruit, wooden chaff and thin river sand are taken, sterilization treatment is carried out to the wooden chaff and thin river sand, sterilized Wooden chaff and the thin river sand that sterilizes, the Chloranthus glaber fruit are plucked between mid-December-early January and obtained, the Chloranthus glaber fruit, The weight of wooden chaff and thin river sand ratio is 100:16:245;
S2. sterilization processing is carried out to the Chloranthus glaber fruit, and adjusts pH value to 5.7, obtain sterilizing Chloranthus glaber fruit;
S3. the sterilization Chloranthus glaber fruit is admixed in the wooden chaff of sterilizing, is well mixed to obtain Chloranthus glaber fruit mixture, Relative humidity with sterilized water regulation coral fruit mixture is 77%, then moves into lucifuge in the storeroom that temperature is 5 DEG C and puts Put 55 days, storage keeps the water content of coral fruit mixture during placing be 77%, obtains Chloranthus glaber fruit mixture;
S4. the Chloranthus glaber fruit mixture is admixed in the thin river sand of sterilizing, is well mixed, moves into vernalization greenhouse Place 16 days, keep the temperature in vernalization greenhouse to obtain the Chloranthus glaber kind that shows money or valuables one carries unintentionally in 19 DEG C, relative humidity 77% during placement Son.
In the present embodiment step S1, the method for the sterilization treatment is as follows:The wooden chaff and thin river sand are subjected to damp and hot go out Bacterium, wherein the condition of the moist heat sterilization is:Pressure is 109.9kPa, and temperature is 122.0 DEG C, maintains 28 minutes, takes out, be put into Oven, carries out hot air sterilization, and the condition of the hot air sterilization is:Temperature is 157 DEG C, and the time is 1.8 hours, is taken out.
In the present embodiment step S2, the method for the sterilization processing is as follows:Germicidal solution is prepared, the germicidal solution includes The composition of following mass concentration, 11ppm diethylamino ethanol caproates, 320ppm carbendazim, 54ppm carboxymethyl chitosans, 27ppm brassinosteroids, 27ppm6- benzyladenines, 0.4ppm triacontanols, and pH value is adjusted as 5.7;It is molten to the sterilization The Chloranthus glaber fruit is added in liquid, is soaked 1.2 hours, is taken out.
Embodiment 4
S1. Chloranthus glaber fruit, wooden chaff and thin river sand are taken, sterilization treatment is carried out to the wooden chaff and thin river sand, sterilized Wooden chaff and the thin river sand that sterilizes, the Chloranthus glaber fruit are plucked between mid-December-early January and obtained, the Chloranthus glaber fruit, The weight of wooden chaff and thin river sand ratio is 100:17:265;
S2. sterilization processing is carried out to the Chloranthus glaber fruit, and adjusts pH value to 6, obtain sterilizing Chloranthus glaber fruit;
S3. the sterilization Chloranthus glaber fruit is admixed in the wooden chaff of sterilizing, is well mixed to obtain Chloranthus glaber fruit mixture, Relative humidity with sterilized water regulation coral fruit mixture is 80%, then moves into lucifuge in the storeroom that temperature is 6 DEG C and puts Put 50 days, storage keeps the water content of coral fruit mixture during placing be 80%, obtains Chloranthus glaber fruit mixture;
S4. the Chloranthus glaber fruit mixture is admixed in the thin river sand of sterilizing, is well mixed, moves into vernalization greenhouse Place 17 days, keep the temperature in vernalization greenhouse to obtain the Chloranthus glaber kind that shows money or valuables one carries unintentionally in 22 DEG C, relative humidity 80% during placement Son.
In the present embodiment step S1, the method for the sterilization treatment is as follows:The wooden chaff and thin river sand are subjected to damp and hot go out Bacterium, wherein the condition of the moist heat sterilization is:Pressure is 123.5kPa, and temperature is 124.0 DEG C, maintains 25 minutes, takes out, be put into Oven, carries out hot air sterilization, and the condition of the hot air sterilization is:Temperature is 160 DEG C, and the time is 2 hours, is taken out.
In the present embodiment step S2, the method for the sterilization processing is as follows:Germicidal solution is prepared, the germicidal solution includes The composition of following mass concentration, 11ppm diethylamino ethanol caproates, 350ppm carbendazim, 60ppm carboxymethyl chitosans, 30ppm brassinosteroids, 30ppm6- benzyladenines, 0.5ppm triacontanols, and pH value is adjusted as 6;To the germicidal solution It is middle to add the Chloranthus glaber fruit, soak 1.5 hours, take out.
Embodiment 5
S1. Chloranthus glaber fruit, wooden chaff and thin river sand are taken, sterilization treatment is carried out to the wooden chaff and thin river sand, sterilized Wooden chaff and the thin river sand that sterilizes, the Chloranthus glaber fruit are plucked between mid-December-early January and obtained, the Chloranthus glaber fruit, The weight of wooden chaff and thin river sand ratio is 100:19:280;
S2. sterilization processing is carried out to the Chloranthus glaber fruit, and adjusts pH value to 6.3, obtain sterilizing Chloranthus glaber fruit;
S3. the sterilization Chloranthus glaber fruit is admixed in the wooden chaff of sterilizing, is well mixed to obtain Chloranthus glaber fruit mixture, Relative humidity with sterilized water regulation coral fruit mixture is 83%, then moves into lucifuge in the storeroom that temperature is 8 DEG C and puts Put 45 days, storage keeps the water content of coral fruit mixture during placing be 83%, obtains Chloranthus glaber fruit mixture;
S4. the Chloranthus glaber fruit mixture is admixed in the thin river sand of sterilizing, is well mixed, moves into vernalization greenhouse Place 19 days, keep the temperature in vernalization greenhouse to obtain the Chloranthus glaber kind that shows money or valuables one carries unintentionally in 24 DEG C, relative humidity 83% during placement Son.
In the present embodiment step S1, the method for the sterilization treatment is as follows:The wooden chaff and thin river sand are subjected to damp and hot go out Bacterium, wherein the condition of the moist heat sterilization is:Pressure is 130.5kPa, and temperature is 125.0 DEG C, maintains 22 minutes, takes out, be put into Oven, carries out hot air sterilization, and the condition of the hot air sterilization is:Temperature is 163 DEG C, and the time is 2.2 hours, is taken out.
In the present embodiment step S2, the method for the sterilization processing is as follows:Germicidal solution is prepared, the germicidal solution includes The composition of following mass concentration, 12ppm diethylamino ethanol caproates, 380ppm carbendazim, 68ppm carboxymethyl chitosans, 33ppm brassinosteroids, 33ppm6- benzyladenines, 0.6ppm triacontanols, and pH value is adjusted as 6.3;It is molten to the sterilization The Chloranthus glaber fruit is added in liquid, is soaked 1.8 hours, is taken out.
Embodiment 6
It is using identical Chloranthus glaber seed storage method, difference in embodiment 1, Chloranthus glaber fruit after harvesting, immediately Fruit pulp is peelled off, only retains Chloranthus glaber seed.
Embodiment 7
It is using identical Chloranthus glaber seed storage method, difference in embodiment 1, the wooden chaff and thin river sand, is not entered Row sterilization treatment.
Embodiment 8
It is using identical Chloranthus glaber seed storage method, difference in embodiment 1, does not use bactericidal liquid to Chloranthus glaber fruit It is real to carry out sterilization processing.
Embodiment 9
The Chloranthus glaber seed tag that embodiment 1-5 is obtained is sample 1-5, the Chloranthus glaber seed mark that embodiment 6-8 is obtained It is designated as comparative sample 1-3.
The assay method of embryo rate is:The Chloranthus glaber fruit 30 after step S3 processing in each embodiment is taken at random, removes fruit Follicarpium meat, Chloranthus glaber seed and vertical profile are obtained, using vernier caliper measurement embryo length and endosperm length, according to embryo rate=(kind Embryo length/endosperm length) × 100%, calculate embryo rate.
The assay method of healthy rate is:100, the Chloranthus glaber seed that shows money or valuables one carries unintentionally after step S4 processing in each embodiment is taken at random, Wherein full hard for healthy seed, rot mouldy unhealthy seed, 2 repetitions, according to healthy rate=(healthy seed/ Determine seed sum) × 100%, calculate healthy rate.
The assay method of germination rate is:Random 100, the Chloranthus glaber seed that shows money or valuables one carries unintentionally taken after step S4 processing, using culture dish + bis- filter paper beds, it is placed in constant temperature illumination at 25 DEG C and cultivates, the cycle is 20 days, 2 repetitions, according to germination rate=(germination seed/survey Determine seed sum) × 100%, calculate germination rate.
The assay method of emergence rate:Chloranthus glaber seed after random 300 step S4 processing of picking, is moved into seedling raising greenhouse Nursery is carried out, was sowed April 1 to emergence on April 28 and terminated, statistics emergence result, and the Chloranthus glaber after emergence is carried out Count seedling stage diseases and pests situation, predominantly black points and anthracnose situation.
The measurement result of the Chloranthus glaber seed of each embodiment processing is shown in Table 1.
The Chloranthus glaber seed testing result of table 1
From table 1 it follows that the Chloranthus glaber seed sample 1-5 handled by the inventive method, embryo development is good, substantially Development is complete.Using in the embodiment 6 for removing Chloranthus glaber fruit pulp, nutrient, phase are supplied because Chloranthus glaber seed lacks pulp Than sample 1, the embryo rate in comparative sample 1 reduces 34.87%;With the embodiment 8 using non-sterile wooden chaff and thin river sand Compare, find that mildew phenomena occurs in more Chloranthus glaber seed in comparative sample 2 in measure, compared to sample 1, its healthy rate reduces 47%, thus it is speculated that it is due to that the microorganism that wood chip and river sand are brought causes the mildew phenomena of Chloranthus glaber seed, and the present invention is real Apply a Chloranthus glaber seed for 1-5 processing and integrally present high healthy rate;In embodiment 8, due to not using bactericidal liquid to grass Coral fruit carries out sterilization processing, and compared with the sample 1 in embodiment 1, the embryo rate of comparative sample 3 reduces 25.98%, health Rate reduces 31.50%, and germination rate reduces 24.00%, it was also found that its emergence rate also occurs in seedling raising process 25.33% reduction, and occur more black points, the pest and disease damage situation of anthracnose in seedling stage, and after present invention storage Chloranthus glaber seed, it is few in seedling stage diseases and pests situation, and comprehensive pest and disease damage rate is less than 1.5%.Therefore the grass handled using the present invention Coral seed, have embryo development good, go mouldy few, healthy rate, the characteristics of germination rate is high, while also improve Chloranthus glaber seed In the emergence rate in nursery stage, reduce the disease insect pest situation in Chloranthus glaber seedling stage.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to Cover the scope of the claims in the present invention.

Claims (10)

1. a kind of storage method of Chloranthus glaber seed, it is characterised in that comprise the following steps:
S1. Chloranthus glaber fruit, wooden chaff and thin river sand are taken, sterilization treatment is carried out to the wooden chaff and thin river sand, obtains the wooden chaff that sterilizes With the thin river sand that sterilizes, the Chloranthus glaber fruit is plucked between mid-December-early January and obtained, the Chloranthus glaber fruit, wooden chaff Weight ratio with thin river sand is 100:15-20:230-300;
S2. sterilization processing is carried out to the Chloranthus glaber fruit, and adjusts pH value to 5.5-6.5, obtain sterilizing Chloranthus glaber fruit;
S3. the sterilization Chloranthus glaber fruit is admixed in the wooden chaff of sterilizing, Chloranthus glaber fruit mixture is well mixed to obtain, with nothing The relative humidity of bacterium water regulation coral fruit mixture is 75-85%, then moves into lucifuge in the storeroom that temperature is 3-10 DEG C Place 35-60 days, storage keeps the water content of coral fruit mixture during placing be 75-85%, obtains Chloranthus glaber fruit and mixes Compound;
S4. the Chloranthus glaber fruit mixture is admixed in the thin river sand of sterilizing, is well mixed, moves into vernalization greenhouse and place 15-20 days, the temperature in vernalization greenhouse is kept to obtain the careless coral that shows money or valuables one carries unintentionally in 18-26 DEG C, relative humidity 75-85% during placement Coral seed.
2. the storage method of a kind of Chloranthus glaber seed according to claim 1, it is characterised in that described to go out in step S1 The method of bacterium processing is as follows:The wooden chaff and thin river sand are subjected to moist heat sterilization, wherein the condition of the moist heat sterilization is:Pressure For 103.4-137.3kPa, temperature is 121.3-126.0 DEG C, maintains 20-30 minutes, takes out, be put into oven, done Heat sterilization, the condition of the hot air sterilization are:Temperature is 155-165 DEG C, and the time is 1.5-2.5 hours, is taken out.
3. the storage method of a kind of Chloranthus glaber seed according to claim 1, it is characterised in that described to kill in step S2 The method of bacterium processing is as follows:Germicidal solution is prepared, the germicidal solution includes the composition of following mass concentration, 10-12ppm caproic acids Diethylaminoethanol ester, 300-400ppm carbendazim, 50-70ppm carboxymethyl chitosans, 25-35ppm brassinosteroids, 25- 35ppm6- benzyladenines, 0.3-0.7ppm triacontanols, and it is 5.5-6.5 to adjust pH value;Added into the germicidal solution The Chloranthus glaber fruit, 1-2 hours are soaked, taken out.
4. the storage method of a kind of Chloranthus glaber seed according to claim 1, it is characterised in that in step S3, by weight Part ratio, the weight part ratio of the wooden chaff of sterilizing and the sterilization Chloranthus glaber fruit is 15-20:100.
5. the storage method of a kind of Chloranthus glaber seed according to claim 1, it is characterised in that in step S4, by weight Part ratio, the weight part ratio of the thin river sand of sterilizing and the Chloranthus glaber fruit mixture is 2-2.5:1.
A kind of 6. Chloranthus glaber seed obtained by storage method as the Chloranthus glaber seed any one of claim 1 to 5.
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