CN110452883A - 禽白血病p27蛋白单克隆抗体及其制备方法和应用 - Google Patents
禽白血病p27蛋白单克隆抗体及其制备方法和应用 Download PDFInfo
- Publication number
- CN110452883A CN110452883A CN201910629122.XA CN201910629122A CN110452883A CN 110452883 A CN110452883 A CN 110452883A CN 201910629122 A CN201910629122 A CN 201910629122A CN 110452883 A CN110452883 A CN 110452883A
- Authority
- CN
- China
- Prior art keywords
- avian leukosis
- monoclonal antibody
- cell
- preparation
- albumen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000004668 avian leukosis Diseases 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 210000004408 hybridoma Anatomy 0.000 claims abstract description 22
- 241000713826 Avian leukosis virus Species 0.000 claims abstract description 20
- 238000004321 preservation Methods 0.000 claims abstract description 7
- 238000003745 diagnosis Methods 0.000 claims abstract description 4
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 23
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 23
- 210000004027 cell Anatomy 0.000 claims description 17
- 239000002671 adjuvant Substances 0.000 claims description 13
- 239000000427 antigen Substances 0.000 claims description 9
- 108091007433 antigens Proteins 0.000 claims description 9
- 102000036639 antigens Human genes 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 9
- 210000000683 abdominal cavity Anatomy 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 7
- 230000007910 cell fusion Effects 0.000 claims description 7
- 210000002966 serum Anatomy 0.000 claims description 6
- 230000004927 fusion Effects 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 3
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims description 3
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 3
- 238000004945 emulsification Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 230000003053 immunization Effects 0.000 claims description 3
- 238000002649 immunization Methods 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
- 241000699670 Mus sp. Species 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000012894 fetal calf serum Substances 0.000 claims description 2
- 230000009465 prokaryotic expression Effects 0.000 claims description 2
- 210000004989 spleen cell Anatomy 0.000 claims description 2
- 210000003462 vein Anatomy 0.000 claims description 2
- 241000287828 Gallus gallus Species 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 238000009826 distribution Methods 0.000 abstract description 3
- 230000001900 immune effect Effects 0.000 abstract description 2
- 238000001727 in vivo Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 11
- 206010003445 Ascites Diseases 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 241000700605 Viruses Species 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 238000002965 ELISA Methods 0.000 description 6
- 238000001962 electrophoresis Methods 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241001494479 Pecora Species 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 4
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 238000003119 immunoblot Methods 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000323253 Avian leukosis virus ev/J Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- MUUGCDGGIVCSDT-UHFFFAOYSA-N [NH4+].[NH4+].[O-]S([O-])(=O)=O.CCCCCCCC(O)=O Chemical compound [NH4+].[NH4+].[O-]S([O-])(=O)=O.CCCCCCCC(O)=O MUUGCDGGIVCSDT-UHFFFAOYSA-N 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000002055 immunohistochemical effect Effects 0.000 description 2
- 238000011532 immunohistochemical staining Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 101150115162 p27 gene Proteins 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000711404 Avian avulavirus 1 Species 0.000 description 1
- 208000019838 Blood disease Diseases 0.000 description 1
- 241000589876 Campylobacter Species 0.000 description 1
- 241000288673 Chiroptera Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010015719 Exsanguination Diseases 0.000 description 1
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 238000003453 ammonium sulfate precipitation method Methods 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 108700004025 env Genes Proteins 0.000 description 1
- 101150030339 env gene Proteins 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 108700004026 gag Genes Proteins 0.000 description 1
- 101150098622 gag gene Proteins 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000014951 hematologic disease Diseases 0.000 description 1
- 208000018706 hematopoietic system disease Diseases 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 210000005084 renal tissue Anatomy 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1036—Retroviridae, e.g. leukemia viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910629122.XA CN110452883B (zh) | 2019-07-12 | 2019-07-12 | 禽白血病p27蛋白单克隆抗体及其制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910629122.XA CN110452883B (zh) | 2019-07-12 | 2019-07-12 | 禽白血病p27蛋白单克隆抗体及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110452883A true CN110452883A (zh) | 2019-11-15 |
CN110452883B CN110452883B (zh) | 2020-07-17 |
Family
ID=68482794
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910629122.XA Active CN110452883B (zh) | 2019-07-12 | 2019-07-12 | 禽白血病p27蛋白单克隆抗体及其制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110452883B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113341140A (zh) * | 2021-06-02 | 2021-09-03 | 贵州大学 | 一种检测禽白血病p27的间接elisa方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105198986A (zh) * | 2015-09-10 | 2015-12-30 | 中国农业科学院哈尔滨兽医研究所 | 抗禽白血病病毒p27蛋白单克隆抗体,包含该单克隆抗体的金标试纸条及应用 |
-
2019
- 2019-07-12 CN CN201910629122.XA patent/CN110452883B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105198986A (zh) * | 2015-09-10 | 2015-12-30 | 中国农业科学院哈尔滨兽医研究所 | 抗禽白血病病毒p27蛋白单克隆抗体,包含该单克隆抗体的金标试纸条及应用 |
Non-Patent Citations (3)
Title |
---|
BINGLING YUN等: "Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen", 《JOURNAL OF VIROLOGICAL METHODS》 * |
谢华丽等: "禽白血病病毒p27蛋白单克隆抗体的制备及鉴定", 《动物医学进展》 * |
陈晨等: "抗禽白血病p27抗原单克隆抗体的制备与鉴定", 《中国预防兽医学报》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113341140A (zh) * | 2021-06-02 | 2021-09-03 | 贵州大学 | 一种检测禽白血病p27的间接elisa方法 |
Also Published As
Publication number | Publication date |
---|---|
CN110452883B (zh) | 2020-07-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Sithigorngul et al. | Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon | |
CN106434568B (zh) | 一种抗猪流行性腹泻病毒s蛋白杂交瘤细胞株及其应用 | |
CN112921005B (zh) | 一株杂交瘤细胞株及其产生的犬细小病毒vp2蛋白单克隆抗体和应用 | |
CN111413499A (zh) | 一种检测禽腺病毒i群的间接免疫荧光试剂盒 | |
CN107012127B (zh) | 抗b型肝炎病毒核心抗原的单克隆抗体、其用途以及分泌所述单克隆抗体的杂交瘤细胞 | |
CN105348372A (zh) | 一种猪伪狂犬病毒的检测方法 | |
CN113024666B (zh) | 具有广谱性识别a型流感病毒np蛋白的单克隆抗体及其应用 | |
CN101322844B (zh) | 无病毒非结构蛋白的口蹄疫疫苗及其制备方法 | |
CN105974119B (zh) | G种肠道病毒直接免疫荧光试剂及其试剂盒 | |
CN116804186B (zh) | 一种抗鸡传染性贫血病毒单克隆抗体杂交瘤细胞株、单克隆抗体、试剂或试剂盒及其应用 | |
CN110452883A (zh) | 禽白血病p27蛋白单克隆抗体及其制备方法和应用 | |
CN105548550A (zh) | 检测e种肠道病毒的免疫荧光试剂及其检测试剂盒 | |
CN115960220A (zh) | 特异性结合柯萨奇病毒a6的单克隆抗体及其用途 | |
CN113355291B (zh) | 抗丝状支原体丝状亚种p0071蛋白的单克隆抗体及其应用 | |
KR101080071B1 (ko) | 재조합 n 단백질에 대한 단클론 항체를 이용한 리프트계곡열 경합적 효소결합면역측정법 | |
CN109709330B (zh) | 一种口蹄疫病毒竞争elisa检测试剂盒 | |
CN109295006B (zh) | 分泌口蹄疫病毒非结构蛋白单克隆抗体3a10的杂交瘤细胞系及其应用 | |
CN109897830A (zh) | 马泰勒虫ema1单克隆抗体及其应用 | |
CN104046594B (zh) | 一种羊口疮病毒蛋白orfv086单克隆抗体杂交瘤细胞株2g8d10及其单克隆抗体 | |
CN110161246A (zh) | 区分新城疫病毒样颗粒疫苗免疫血清与野毒感染血清的间接竞争elisa方法及试剂盒 | |
CN113980126B (zh) | 一种多杀性巴氏杆菌毒素单克隆抗体及其阻断elisa试剂盒 | |
CN118047860B (zh) | 一种具有竞争活性的iltv单克隆抗体及其应用 | |
CN112898419B (zh) | 用于检测高致病性prrsv的单克隆抗体及应用 | |
CN108330101A (zh) | 一种杂交瘤细胞株及其产生的单克隆抗体和应用 | |
CN109293748B (zh) | 口蹄疫病毒非结构蛋白3a抗原表位肽及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Liu Lina Inventor after: Luo Ling Inventor after: Li Qiongqiong Inventor after: Luo Qingping Inventor after: Shao Huabin Inventor after: Wen Guoyuan Inventor after: Zhang Rongrong Inventor after: Zhang Tengfei Inventor after: Wang Honglin Inventor after: Lu Qin Inventor after: Wang Hongcai Inventor before: Liu Lina Inventor before: Wang Hongcai Inventor before: Luo Ling Inventor before: Li Qiongqiong Inventor before: Luo Qingping Inventor before: Zhao Zongzheng Inventor before: Shao Huabin Inventor before: Wen Guoyuan Inventor before: Zhang Rongrong Inventor before: Zhang Tengfei Inventor before: Wang Honglin Inventor before: Lu Qin |