CN110403063A - A kind of locust tree leaf fermentation material and its preparation method and application - Google Patents
A kind of locust tree leaf fermentation material and its preparation method and application Download PDFInfo
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- CN110403063A CN110403063A CN201910831251.7A CN201910831251A CN110403063A CN 110403063 A CN110403063 A CN 110403063A CN 201910831251 A CN201910831251 A CN 201910831251A CN 110403063 A CN110403063 A CN 110403063A
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- tree leaf
- locust tree
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- fermentation material
- soy sauce
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 235000013555 soy sauce Nutrition 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 22
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- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
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- 235000013325 dietary fiber Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
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- 229920001277 pectin Polymers 0.000 description 3
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- 238000012545 processing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
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- 235000005822 corn Nutrition 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
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- 235000019820 disodium diphosphate Nutrition 0.000 description 2
- GYQBBRRVRKFJRG-UHFFFAOYSA-L disodium pyrophosphate Chemical compound [Na+].[Na+].OP([O-])(=O)OP(O)([O-])=O GYQBBRRVRKFJRG-UHFFFAOYSA-L 0.000 description 2
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- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
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- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
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- 230000003031 feeding effect Effects 0.000 description 1
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- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
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- 239000006260 foam Substances 0.000 description 1
- 244000038280 herbivores Species 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
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- 239000002893 slag Substances 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- GCLGEJMYGQKIIW-UHFFFAOYSA-H sodium hexametaphosphate Chemical compound [Na]OP1(=O)OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])O1 GCLGEJMYGQKIIW-UHFFFAOYSA-H 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/139—Diacetylactis
Abstract
The invention discloses a kind of locust tree leaf fermentation material and its preparation method and application, fermentation material is to be mixed by one time fermentation liquid with locust tree leaf, and assisted fermentation agent, adsorbent and water-retaining agent is added, and carries out secondary fermentation and is made；Wherein, one time fermentation liquid is to be mixed by yellow serofluid and soy sauce residues, and mixed bacteria is added, and carries out one time fermentation and is made.The present invention effectively increases the method for the by-product utilizeds such as yellow serofluid, soy sauce residues, reduce environmental pollution, greatly reduce business processes cost, salinity, protein and minerals etc. in a large amount of water-soluble amino acids, polysaccharide, isoflavones and soy sauce residues are made full use of in yellow serofluid, and improve the level of resources utilization；Use the locust tree leaf fermentation material as feed, reduce dependence of the animal feed to protein feeds such as Soybean Meaies, the resistivity of animal can also be improved, it is beneficial to animal intestinal tract health, and low in cost, long shelf-life, the energy consumption without stoving process, energy-saving and emission-reduction are truly realized, by-product full dose efficiently utilizes.
Description
Technical field
The present invention relates to fermentation techniques and field of fodder, and in particular to a kind of locust tree leaf fermentation material and preparation method thereof and
Using.
Background technique
Traditional soybean product can generate a large amount of yellow serofluid when processing, yellow serofluid itself contains certain protein, water-soluble ammonia
Base acid, polysaccharide etc. naturally contain certain microorganism since its moisture content is high, nutriment is easily absorbed and utilized, in 2-4h not
Processing, extremely easy corruption is smelly, and discharge far can not reach national emission standard naturally, and meeting stench, water nutrition are led
Serious problem of environmental pollution is caused, direct emission yellow serofluid COD value reaches 20000-35000mg/L, and total solid matters reaches
25000-32000mg/L, thousand sheets yellow serofluid suspension substance reach 3582.3mg/L.Protein contained in yellow serofluid is mostly
Soybean whey protein, isoflavones, soyabean oligosaccharides, extracting method is more at present, as soybean whey protein mainly has ultrafiltration
Method, flocculence, foam method etc., but relative cost is high, yield is low, of poor benefits, content is low and extraction cost is too high, has no at present
Report of the technology in production application is difficult to realize industrialization under current technical status.
Soy sauce residues refer to that the by-product that left behind after traditional fermented soybean soy sauce, China's Large-scale enterprises soy sauce in 2018 produce
Amount is 589.04 ten thousand t, produces at least about 4,000,000 t soy sauce residues.The content of soy sauce residues Zhong Shui and salt is very high, and place one section when
Between after be easy to happen rotten fouling, at present major part residue of soya be disposed off as rubbish.The primary raw material of sauce fermentation is soybean,
The comparision contents of crude protein, crude fat and dietary fiber are abundant in soy sauce residues, are to do the very good material of feed, but due to its salt
Divide content higher, limits its application in terms of feed.Soy sauce residues have as fertilizer utilization at present, containing higher
Salinity be easy to cause the salinization of soil, and seriously affects water quality, and fails to efficiently use the nutritional ingredients such as its protein, causes
The wasting of resources.If usually there are problems that moisture is high, salinity is high and not comprehensive nutrition separately as feed, should not being directly fed with,
It needs to be cleaned with clear water, additionally causes environment and cost burden, to using bringing inconvenience and increase cost.
Locust tree leaf protein rich in, minerals and vitamins, it is fine that anti-nutritional factors is few, and palatability is good
Feed resource, but due to acquisition, processing difficulties, common processing method is such as dried, be milled the high requirements on the equipment, energy consumption
Height leads at high cost, bad operation, time-consuming bothersome, causes currently without the good Land use systems of formation.Locust tree leaf nutriture value
Value is high, and feeding Folium sophorae can accelerate livestock and poultry weight gain, improve the price of deed, and locust tree leaf fragrant taste itself is sweet, and palatability is good,
Rich in vitamin and several mineral materials, there are heat-clearing toxin-expelling functions.When spring and summer locust tree leaf protein content is high, tunning can
To replace pig, birds feed a part, after the decline of autumn leaf protein, ox can be used as, the part of sheep Herbivore replaces
Feed.
Summary of the invention
The purpose of the present invention is to provide a kind of locust tree leaf fermentation materials and preparation method thereof, utilize yellow serofluid and soy sauce
Slag carries out half sponge process and prepares locust tree leaf fermentation material, and using the fermentation material as the substitute of feed a part.
The present invention through the following technical solutions to achieve the above objectives:
A kind of locust tree leaf fermentation material, the fermentation material are to be mixed by one time fermentation liquid with locust tree leaf, and auxiliary is added
Leavening, adsorbent and water-retaining agent carry out secondary fermentation and are made；Wherein, the one time fermentation liquid is by yellow serofluid and soy sauce residues
Mixing, and mixed bacteria is added, it carries out one time fermentation and is made.
Further improvement lies in that the assisted fermentation agent includes cellulase, hemicellulase and pectase.
Further improvement lies in that the adsorbent selects one of wheat bran, corn flour or calcium carbonate or a variety of.
Further improvement lies in that the water-retaining agent selects composite phosphate, such as sodium tripolyphosphate, calgon, coke
Sodium phosphate, tertiary sodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium acid pyrophosphate, Sodium Acid Pyrophosphate etc..
Further improvement lies in that the mixed bacteria includes lactobacillus lactis, lactobacillus acidophilus, bacillus subtilis, ferment
Female bacterium and mould, the various Pseudomonas ratios of Various Seasonal have adjustment.
The present invention also provides a kind of preparation method of locust tree leaf fermentation material, step includes
Step 1: collecting yellow serofluid and soy sauce residues respectively, (determine according to the salt content of soy sauce residues according to the mass ratio of 8-10:1
Fixed specific ratio, every production firm's soy sauce residues ratio are substantially stationary) yellow serofluid and soy sauce residues are mixed, and Mixed Microbes are added
Kind, one time fermentation is carried out, ferment 3-7 days obtained one time fermentation liquid, can save after fermentation 9-12 months；
Step 2: acquisition locust tree leaf, may include part spray, is cut into the segment or fractionlet of 1-2cm, by locust tree tree
Leaf divides layer 2-3 to pile up, and every layer, with a thickness of 20-25cm, is successively uniformly sprayed one time fermentation liquid, controls locust tree leaf and one time fermentation
The mass ratio of liquid is 10:1-1.5, and assisted fermentation agent, adsorbent and water-retaining agent is added, secondary fermentation is carried out at 25-30 DEG C, is made
Obtain locust tree leaf fermentation material.
Further improvement lies in that the acquisition time of the locust tree leaf is to start to mid or late October annual May, in morning
The morning and at dusk acquisition.Specific acquisition mode diversification, it is proposed that downgrade 1m or so stub, single in plexi or full line in tree
Hedge shape, every row can side by side 2-3, are conducive to collective's pumping favour and go out leaf acquisition.
Further improvement lies in that turning in 2-4 days is primary during the secondary fermentation, and after 2-3 turning, control
Moisture processed can be harvested in 30-35%, obtain the product with fermentation paste flavor taste, and spare, every bag of 50kg is sealed in pack sealing up for safekeeping.
When summer temp is higher than 36 DEG C, pay attention to aeration-cooling, can suitably increase turn pile frequency.
Further improvement lies in that the dosage of the adsorbent is the 0.5-1% of locust tree leaf quality, the use of the water-retaining agent
Amount is the 0.3-0.5% of locust tree leaf quality.
The present invention also provides a kind of above-mentioned locust tree leaf fermentation materials as the application in feed substitute, by locust tree tree
Leaf fermentation material is individually fed with or is fed with after mixing with concentrated feed.In pig and birds use process, it is proposed that additive amount is no more than
30%, the phytophage such as cattle and sheep rabbit can not limit amount, it is contemplated that growth efficiency, it is proposed that feeding amount be no more than 60%.
The beneficial effects of the present invention are: the method not only effectively increases the method for by-product utilized and reduces environment
Pollution, greatly reduces business processes cost, makes full use of a large amount of water-soluble amino acids, polysaccharide, isoflavones and sauce in yellow serofluid
Salinity, protein and minerals etc. in the dregs of fat, and improve the level of resources utilization；Also, yellow serofluid, soy sauce residues, locust tree leaf
Contain flavonoids natural secondary metabolite, yellow serofluid provides the nutrition of bacterium colony growth and culture medium effect, soy sauce residues provide
The salinities such as protein source, minerals and part bacterium source source, locust tree leaf provide the main bodys nutritional ingredients such as protein；Use this
Locust tree leaf fermentation material reduces dependence of the animal feed to protein feeds such as Soybean Meaies, can also improve animal as feed
Resistivity is beneficial to animal intestinal tract health, and low in cost, long shelf-life is truly realized without the energy consumption of stoving process
Energy-saving and emission-reduction, by-product full dose efficiently utilize, and are conducive to the industrial development direction of green cultivation and nonreactive feed.
Specific embodiment
The application is described in further detail below with reference to embodiment, it is necessary to it is indicated herein to be, it is real in detail below
The mode of applying is served only for that the application is further detailed, and should not be understood as the limitation to the application protection scope, the field
Technical staff some nonessential modifications and adaptations can be made to the application according to above-mentioned application content.
Embodiment 1
A kind of locust tree leaf fermentation material, uses following preparation method, and step includes:
Step 1: collecting yellow serofluid and soy sauce residues respectively, and yellow serofluid and soy sauce residues are mixed by the mass ratio of 8:1, and adds
Enter the mixed bacteria including lactobacillus lactis, lactobacillus acidophilus, bacillus subtilis, saccharomycete and mould, carry out one time fermentation,
The fermentation phase is 3 days, obtains one time fermentation liquid.
Step 2: using gardens plant harvester, in the early morning and dusk acquisition locust tree leaf that May is daily, and
The section or broken lobate of 1cm is chopped into after acquisition, by points of 2 layers stacking of locust tree leaf in fermentation vat, every layer with a thickness of 25cm, successively
The mass ratio of uniformly sprinkling one time fermentation liquid, control locust tree leaf and one time fermentation liquid is 10:1, and control fermentation temperature is 25 DEG C,
The assisted fermentation agent including cellulase, hemicellulase and pectase is added, cellulose, hemicellulose in locust tree leaf of degrading
Element and pectin, are additionally added the wheat bran for accounting for locust tree leaf quality 0.5% as adsorbent, and account for locust tree leaf quality 0.3%
Composite phosphate water-retaining agent carries out secondary fermentation, and during secondary fermentation, turning in 2 days is primary, after 3 turnings, control
Moisture can be harvested 30%, and locust tree leaf fermentation material is made.
Embodiment 2
A kind of locust tree leaf fermentation material, uses following preparation method, and step includes:
Step 1: collecting yellow serofluid and soy sauce residues respectively, and yellow serofluid and soy sauce residues are mixed by the mass ratio of 9:1, and adds
Enter the mixed bacteria including lactobacillus lactis, lactobacillus acidophilus, bacillus subtilis, saccharomycete and mould, carry out one time fermentation,
The fermentation phase is 5 days, obtains one time fermentation liquid.
Step 2: using gardens plant harvester, in August part daily early morning and dusk acquisition locust tree leaf, and
The section or broken lobate of 1.5cm is chopped into after acquisition, by points of 2 layers stacking of locust tree leaf in fermentation vat, every layer with a thickness of 20cm, by
Layer uniformly sprays one time fermentation liquid, and the mass ratio for controlling locust tree leaf and one time fermentation liquid is 10:1.2, and control fermentation temperature is
28 DEG C, be added include cellulase, hemicellulase and pectase assisted fermentation agent, degrade locust tree leaf in cellulose,
Hemicellulose and pectin are additionally added the corn flour for accounting for locust tree leaf quality 0.8% as adsorbent, and account for locust tree leaf quality
0.4% composite phosphate water-retaining agent carries out secondary fermentation, and during secondary fermentation, turning in 3 days is primary, by 2 turnings
Afterwards, control moisture can be harvested 32%, and locust tree leaf fermentation material is made.
Embodiment 3
A kind of locust tree leaf fermentation material, uses following preparation method, and step includes:
Step 1: collecting yellow serofluid and soy sauce residues respectively, and yellow serofluid and soy sauce residues are mixed by the mass ratio of 10:1, and adds
Enter the mixed bacteria including lactobacillus lactis, lactobacillus acidophilus, bacillus subtilis, saccharomycete and mould, carry out one time fermentation,
The fermentation phase is 7 days, obtains one time fermentation liquid.
Step 2: using gardens plant harvester, in the early morning and dusk acquisition locust tree tree that mid or late October is daily
Leaf, and be chopped into the section or broken lobate of 2cm after acquiring, by 3 layers of stacking of locust tree leaf point in fermentation vat, every layer with a thickness of
20cm successively uniformly sprays one time fermentation liquid, and the mass ratio for controlling locust tree leaf and one time fermentation liquid is 10:1.5, and control is fermented
Temperature is 30 DEG C, and the assisted fermentation agent including cellulase, hemicellulase and pectase is added, the fibre in locust tree leaf of degrading
Dimension element, hemicellulose and pectin, are additionally added the calcium carbonate for accounting for locust tree leaf quality 1% as adsorbent, and account for locust tree leaf
The composite phosphate water-retaining agent of quality 0.5% carries out secondary fermentation, and during secondary fermentation, turning in 4 days is primary, by 2 times
After turning, moisture is controlled 35%, can be harvested, locust tree leaf fermentation material is made.
Composition detection is carried out to fermentation material made from above three embodiments, its protein raising 7-15% is measured and (compares
Compared with untreated yellow serofluid, soy sauce residues and locust tree leaf average content), up to 47.52%, salt content 0.2-
0.3%, far below the requirement of feed diet 1%, the collocation and feeding with other feeds are not influenced.Also, the fermentation material is flat
Equal PH is 5.1, and lysine 2.76%, crude fat 6.58%, crude fibre 16.3%, cinder is divided into 8.6%, is had higher
Acid protease, saccharification enzyme activity and cellulase activity.
The present invention also provides using above-mentioned fermentation material as the application of feed substitute, fermentation material can be mixed with concentrated feed
Feeding, can also individually be fed with, in pig and birds use process, it is proposed that additive amount is no more than 30%, the phytophage such as cattle and sheep rabbit
Can not limit amount, it is contemplated that growth efficiency, it is proposed that feeding amount be no more than 60%.Use the locust tree leaf fermentation material as
Feed sub is beneficial to animal intestinal tract health, and low in cost, long shelf-life is really done without the energy consumption of stoving process
To energy-saving and emission-reduction, by-product full dose is efficiently utilized.
In order to verify feeding effect of the fermentation material prepared by the present invention as animal feed, progress following comparative testing:
It tests group: 1. embodiment group: being carried out using fermentation material made from embodiment 2 and the mixed fodder of common roughage
Feeding, and mixed weight ratio is fermentation material: normal diet=1:4；2. control group: common roughage.
Experimentation: 40 hogs, 10 pregnant sows and 40 cow heads are chosen, are randomly divided into two groups, every group is 20
Head hog, 5 pregnant sows and 20 cow heads, using the mixed fodder of embodiment group and the normal diet of control group respectively to two
Group livestock is fed with, and is detected and obtained following data:
It can be seen that in table
(1) growing and fattening pigs are fed with, the embodiment group feed-weight ratio that is averaged by the 2.78 of control group drops to 2.54, improves feed
Utilization benefit；
(2) be fed with pregnant sow after, embodiment group compared with the control group, weaned piglet litter weight increase by 2.63%, wean at
Motility rate improves 1.4%, and enviromental ammonia declines 1.9ppm；
(3) after being fed with milk cow 30 days, compared with the control group, average milk production increases to 9.55Kg by 8.6Kg to embodiment group,
Lactose content increases to 4.98% by 4.7%, Immunoglobulin IgA in milk, IgG, IgM increased separately 34.06%,
21.6% and 17.3%, it is very big to improve immunoglobulin content in milk.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.
Claims (10)
1. a kind of locust tree leaf fermentation material, it is characterised in that: the fermentation material be mixed by one time fermentation liquid with locust tree leaf, and
Assisted fermentation agent, adsorbent and water-retaining agent is added, carries out secondary fermentation and is made；Wherein, the one time fermentation liquid is by yellow serofluid
It is mixed with soy sauce residues, and mixed bacteria is added, carried out one time fermentation and be made.
2. a kind of locust tree leaf fermentation material according to claim 1, it is characterised in that: the assisted fermentation agent includes fiber
Plain enzyme, hemicellulase and pectase.
3. a kind of locust tree leaf fermentation material according to claim 1, it is characterised in that: the adsorbent selects wheat bran, jade
One of rice flour or calcium carbonate are a variety of.
4. a kind of locust tree leaf fermentation material according to claim 1, it is characterised in that: the water-retaining agent selects compound phosphoric acid
Salt.
5. a kind of locust tree leaf fermentation material according to claim 1, it is characterised in that: the mixed bacteria includes lactic acid cream
Bacillus, lactobacillus acidophilus, bacillus subtilis, saccharomycete and mould.
6. a kind of preparation method of locust tree leaf fermentation material, it is characterised in that: step includes
Step 1: collecting yellow serofluid and soy sauce residues respectively, yellow serofluid and soy sauce residues are mixed according to the mass ratio of 8-10:1, and adds
Enter mixed bacteria, carry out a sealed fermenting, ferment 3-7 days obtained one time fermentation liquid, this fermentation liquid can save 6 under room temperature
Month；
Step 2: acquisition locust tree leaf, it may include part spray is cut into the segment or fractionlet of 1-2cm, divides locust tree leaf to 2-
3 layers of stacking, every layer, with a thickness of 20-25cm, is successively uniformly sprayed one time fermentation liquid, controls the matter of locust tree leaf and one time fermentation liquid
Amount is added assisted fermentation agent, adsorbent and water-retaining agent, secondary fermentation is carried out at 25-30 DEG C, locust tree is made than being 10:1-1.5
Leaf fermentation material.
7. a kind of preparation method of locust tree leaf fermentation material according to claim 6, it is characterised in that: the locust tree leaf
Acquisition time be to start annual May to mid or late October, the noon and acquire at dusk in the morning.
8. a kind of preparation method of locust tree leaf fermentation material according to claim 6, it is characterised in that: the secondary fermentation
During, turning in 2-4 days is primary, after 2-3 turning, controls moisture in 30-35%, can harvest.
9. a kind of preparation method of locust tree leaf fermentation material according to claim 6, it is characterised in that: the adsorbent
Dosage is the 0.5-1% of locust tree leaf quality, and the dosage of the water-retaining agent is the 0.3-0.5% of locust tree leaf quality.
10. a kind of locust tree leaf fermentation material a method as claimed in any one of claims 1 to 5 is as the application in feed substitute.
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