CN110402937A - A kind of microbicide compositions and its application of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin - Google Patents

A kind of microbicide compositions and its application of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin Download PDF

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CN110402937A
CN110402937A CN201910765097.8A CN201910765097A CN110402937A CN 110402937 A CN110402937 A CN 110402937A CN 201910765097 A CN201910765097 A CN 201910765097A CN 110402937 A CN110402937 A CN 110402937A
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dimoxystrobin
bacterium amine
biphenyl
microbicide compositions
wheat
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CN110402937B (en
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高新菊
王恒亮
蒋水萍
董淑静
祖均怀
陈威
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Institute of Plant Protection of Henan Academy of Agricultural Sciences
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Institute of Plant Protection of Henan Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to technical field of pesticide, and in particular to a kind of composition of the fungicide of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin.The effective component of the microbicide compositions is biphenyl pyrrole bacterium amine and dimoxystrobin, and the weight ratio of biphenyl pyrrole bacterium amine and dimoxystrobin is (30:1)~(1:30).The present invention is used in combination using the fungicide biphenyl pyrrole bacterium amine and dimoxystrobin of different role mechanism, has apparent synergistic effect to wheat powdery mildew, wheat rust and wheat scab, and effectively control pathogenic microorganisms develops drug resistance；Field trial shows when the proportion of biphenyl pyrrole bacterium amine and dimoxystrobin is 10:1, after 7 days, 93.36% is up to the preventive effect of wheat rust, 94.04% is up to the preventive effect of wheat powdery mildew, 91.57% is up to the preventive effect of wheat scab, is all remarkably higher than biphenyl pyrrole bacterium amine single dose and dimoxystrobin single dose.

Description

A kind of microbicide compositions and its application of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin And its application.
Background technique
Biphenyl pyrrole bacterium amine (Bixafen), chemical formula C18H12Cl2F3N3O, chemical name are that (3 ', 4 '-two is chloro- by N- 5- fluorine hexichol -2- base) -3- (difluoromethyl) -1- methylpyrazole -4- formamide.Biphenyl pyrrole bacterium amine is pyrazoles fungicide, is one The systemic fungicide that kind novel wide spectrum, protection and therapeutic activity have concurrently.Biphenyl pyrrole bacterium amine passes through interference pathogen mitochondria The succinate dehydrogenase on Complex II in respiratory electron transport chain inhibits mitochondrial function, it is prevented to generate energy, inhibits Growth of pathogenic bacteria eventually leads to its death.Biphenyl pyrrole bacterium amine is systemic fungicide, has extensive fungicidal spectrum, is exclusively used in leaf Face is spraying.Test proves that biphenyl pyrrole bacterium amine presents excellent preventive effect to many diseases on wheat crops, as wheat leaf blight, Leaf rust, stripe rust, powdery mildew, head blight etc., and the shell needle that resistance is generated to methoxy acrylic bactericide can be prevented and treated Leaf spot caused by spore category pathogen etc..It is reported that biphenyl pyrrole bacterium amine has good treatment to net blotch of barley, apple mildew And protecting effect.
Dimoxystrobin (Dimoxystrobin), molecular formula C19H22N2O3, chemical name are that (methoxyl group is sub- by (E) -2- Amino)-N- methyl -2 [a- (2,5- xylyloxy)-o- tolyl] acetamide.Dimoxystrobin is the outer inhibitor of quinone, and bonding is thin The oxidation site of the reduced coenzyme Q of born of the same parents' pigment B also becomes Q0 inhibitor to inhibit the respiration of mitochondria, thus dry The intracorporal energy circulation of fungi is disturbed, there is protectiveness, therapeutic and conductibility, spore germination and mycelia is inhibited to grow.
Wheat rust, powdery mildew, head blight are middle and later periods Major Diseases in Wheat Production, a kind of fungicide pair of single use The killing effect of pest and disease damage is limited, and single bactericide is in use for some time, is easy to cause pathogenic microorganism to generate anti- Pharmacological property, the effect for killing pest and disease damage reduce.
Summary of the invention
The purpose of the present invention is to provide microbicide compositions and its application of a kind of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin, with The killing effect to wheat powdery mildew, rust and head blight is improved, is compounded using the different effective component of two kinds of mechanisms of action, it is more Target site acts on pathogenic microorganism, and pathogenic microorganisms is avoided to develop drug resistance.
Based on above-mentioned purpose, the present invention adopts the following technical scheme: the fungicide of a kind of pyrrole containing biphenyl bacterium amine and dimoxystrobin Composition, the effective component of the microbicide compositions are biphenyl pyrrole bacterium amine and dimoxystrobin, the weight of biphenyl pyrrole bacterium amine and dimoxystrobin Than for (30:1)~(1:30).
Further, the weight ratio of biphenyl pyrrole bacterium amine and dimoxystrobin is (20:1)~(1:10).
Further, biphenyl pyrrole bacterium amine and dimoxystrobin total weight ratio shared in the microbicide compositions gross weight are 10%~80%.
Further, microbicide compositions are any one in suspending agent, missible oil, wettable powder and water dispersible granules Kind.
It further, further include auxiliary agent in microbicide compositions；The auxiliary agent is wetting agent, dispersing agent, filler, emulsification One of agent, thickener, disintegrating agent, binder, antifreezing agent and solvent are a variety of.
Further, microbicide compositions are suspending agent type, weight composition are as follows: biphenyl pyrrole bacterium amine and dimoxystrobin weight it Be 10~50%, emulsifier 3~8%, thickener 0.5~2%, antifreezing agent 3%~8%, dispersing agent 3%~5%, water is supplied To 100%.
Further, microbicide compositions are missible oil type, weight composition are as follows: the sum of biphenyl pyrrole bacterium amine and dimoxystrobin weight It is 10%~40%, emulsifier 9%~15%, solvent complements to 100%.
Further, microbicide compositions are water dispersible granules, weight composition are as follows: biphenyl pyrrole bacterium amine and dimoxystrobin weight The sum of be 10%~80%, wetting agent 3~6%, dispersing agent 3~5%, disintegrating agent 2~6%, binder 0.5~3%, filler mend Enough to 100%.
Further, microbicide compositions are wettable powder, weight composition are as follows: biphenyl pyrrole bacterium amine and dimoxystrobin weight The sum of be 10%~60%, wetting agent 5~15%, dispersing agent 3%~15%, filler complements to 100%.
Further, wetting agent is sldium lauryl sulfate, Nekal BX, lauryl sodium sulfate and dodecyl benzene sulfonic acid Sodium is any one or more of；Dispersing agent is sodium metnylene bis-naphthalene sulfonate, lignin calcium carbonate, alkylnaphthalene sulfonate, alkyl phenol Polyoxyethylene ether is any one or more of；Filler is any one of white carbon black, kaolin, bentonite and diatomite or more Kind；Emulsifier is any one of phenethyl phenol polyoxyethylene ether, fatty alcohol block polyether, NP-7 and agriculture breast series or more Kind；Thickener is gelatin, xanthan gum, hydroxymethyl cellulose, hydroxyethyl cellulose, polyvinylpyrrolidone, polyvinyl acetate It is any one or more of；Disintegrating agent is that sodium chloride, urea, ammonium sulfate, sodium carbonate are any one or more of；Binder is poly- Vinyl alcohol, polyethylene glycol, soluble starch are any one or more of；Antifreezing agent is ethylene glycol, propylene glycol, any of glycerine Kind is a variety of；Solvent is any one or more of solvent naphtha, pyrrolidones, NN dimethylformamide or methyl oleate.
If the fungicide of the above-mentioned bacterium amine of pyrrole containing biphenyl and dimoxystrobin is in prevention and treatment wheat powdery mildew, rust and head blight Using.
Compared with prior art, the invention has the benefit that
1. the present invention is combined using by biphenyl pyrrole bacterium amine and dimoxystrobin compounding, to wheat powdery mildew, wheat rust and wheat Head blight has obvious synergistic function；Especially when the proportion of biphenyl pyrrole bacterium amine and dimoxystrobin is 10:1, to wheat Powdery mildew co-toxicity coefficient is up to 171.87, is up to 155.21 to wheat rust co-toxicity coefficient, reaches to wheat scab co-toxicity coefficient To 149.11, it is seen then that the two has significantly more increasing to wheat powdery mildew, wheat rust and wheat scab by 10:1 mixture Effect effect.
2. field trial shows when the weight ratio of biphenyl pyrrole bacterium amine and dimoxystrobin is 10:1, after 7 days, to wheat The preventive effect of rust is up to 93.36%, is up to 94.04% to the preventive effect of wheat powdery mildew, is up to the preventive effect of wheat scab 91.57%, it is all remarkably higher than biphenyl pyrrole bacterium amine single dose and dimoxystrobin single dose.
3. biphenyl pyrrole bacterium amine passes through the succinic acid on the Complex II in interference pathogen mitochondrial respiratory electron transport chain Dehydrogenase inhibits mitochondrial function, it is prevented to generate energy, inhibits growth of pathogenic bacteria, eventually leads to its death；Dimoxystrobin is quinone Outer inhibitor, is bonded the oxidation site of the reduced coenzyme Q of Cytochrome B, to inhibit the respiration of mitochondria, also referred to as Q0 inhibitor interferes the intracorporal energy circulation of fungi, so that spore germination and mycelia be inhibited to grow；The present invention uses micro- to cause of disease The different biphenyl pyrrole bacterium amine of Biological Action and dimoxystrobin compounding combination, the more than enough target site of energy act on pathogenic microorganism, To which effectively control pathogenic microorganisms develops drug resistance.
Specific embodiment
The microbicide compositions of pyrrole containing biphenyl the bacterium amine and dimoxystrobin of 1 suspending agent of embodiment
According to table 1, water, emulsifier, dispersing agent, antifreezing agent are added in mixing kettle and are stirred, is uniformly mixed, then by biphenyl pyrrole Bacterium amine and dimoxystrobin are put into mixing kettle, are stirred evenly, and then by shearing, sand milling, then are uniformly mixed, are prepared with thickener The suspending agent type microbicide compositions of a series of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin, number is X-1~X-7 respectively.
Wherein, A is writing a Chinese character in simplified form for biphenyl pyrrole bacterium amine；B is writing a Chinese character in simplified form for dimoxystrobin；A:B indicates biphenyl pyrrole bacterium amine and dimoxystrobin Weight ratio；(A+B) % indicates the sum of weight of biphenyl pyrrole bacterium amine and dimoxystrobin shared hundred in microbicide compositions total weight Divide ratio.The meaning of A, B are same as above in other embodiments.
Meanwhile control sample DX-1~DX-4 is prepared referring to the above method, in case follow-up test uses.
The specific formula of the microbicide compositions of the different suspending agent types of table 1
The microbicide compositions of pyrrole containing biphenyl the bacterium amine and dimoxystrobin of 2 missible oil type of embodiment
According to table 2, NN dimethylformamide, agriculture breast 1602 are put into mixing kettle, stirred evenly, then by biphenyl pyrrole bacterium amine It puts into mixing kettle and stirs evenly with dimoxystrobin, be in homogeneous and transparent liquid, prepare a series of bacterium amine of pyrrole containing biphenyl and ether bacterium The missible oil type microbicide compositions of amine, number is R-1~R-7 respectively.
The specific formula of the microbicide compositions of the different missible oil types of table 2
The microbicide compositions of pyrrole containing biphenyl the bacterium amine and dimoxystrobin of 3 water dispersible granules of embodiment
According to tables 3, biphenyl pyrrole bacterium amine, dimoxystrobin, filler are uniformly mixed, crushed through airslide disintegrating mill, then with wetting agent, Dispersing agent, disintegrating agent, binder mixing are added water according still further to the 10% of said mixture total weight, are uniformly mixed, by squeezing Be granulated, dry, sieve etc., the water dispersible granules microbicide compositions of a series of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin are prepared, respectively Number is S-1~S-7.
The specific formula of the microbicide compositions of 3 different in moisture shot dosage form of table
Note: granulation is separately added with water according to 10% of formula gross weight in table 3, and removal is dried after granulation, therefore not in table 3 It lists.
The microbicide compositions of pyrrole containing biphenyl the bacterium amine and dimoxystrobin of 4 wettable powder of embodiment
According to table 4, biphenyl pyrrole bacterium amine, dimoxystrobin, wetting agent, dispersing agent, filler are uniformly mixed, through airslide disintegrating mill powder It is broken, the Wettable powdered disinfectant composition of a series of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin is prepared, number is S-1~S-7 respectively.
The specific formula of the microbicide compositions of the different wettable powder dosage forms of table 4
5 indoor toxicity determination test of embodiment
The test method and experimental result of wheat powdery mildew toxicity test are directed in Room 5.1
Since the indexs such as virulence equation, EC50, co-toxicity coefficient in toxicity test test result are mainly effective with medicament Ingredient and effective component compound proportion are related, influenced by the formulations of pesticide it is smaller, therefore the present invention select suspending agent type antimicrobial combination Object inquires into whether effective component compounding has synergistic effect as exemplary dosage form.
1. measuring method
1) warm indoor pot culture wheat powdery mildew diseased plant.
2) to have infected the wheat of wheat powdery mildew as test strain, if 1 clear water control, 13 chemicals treatments；13 Chemicals treatment respectively correspond biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose, the microbicide compositions of number X-1~X-7, DX-1~ 6 concentration gradients are arranged in the microbicide compositions of DX-4, each processing, be followed successively by 32mg/L, 16mg/L, 8mg/L, 4mg/L, 2mg/L and 1mg/L；4 repetitions are arranged in each concentration gradient.
3) spraying treatment is carried out with Potter spray tower, spouting liquid 450L/ha is control with clear water, each for calculating Virulence equation, EC50 and the co-toxicity coefficient of medicament.
4) after being administered 15d, control efficiency is calculated with disease index, it is for statistical analysis with DPS data processing software, it finds out Virulence regression equation calculates the EC50 of each medicament, calculates co-toxicity coefficient by Sun Yunpei's Method.
5) virulence point is carried out to wheat powdery mildew by microbicide compositions and biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose Analysis, the results are shown in Table 5.
Biphenyl pyrrole bacterium amine is with dimoxystrobin different ratio to the Virulent Analysis of wheat powdery mildew in 5 suspending agent type fungicide of table
2. interpretation of result
By the data in table 5 it is found that biphenyl pyrrole bacterium amine and dimoxystrobin single dose are to the EC of wheat powdery mildew50Value is respectively 3.36mg/L, 2.91mg/L, the EC of the microbicide compositions of number X-1~X-7 to wheat powdery mildew50Value is respectively 2.33mg/ L, 2.18mg/L, 1.93mg/L, 2.01mg/L, 1.99mg/L, 2.24mg/L and 2.33mg/L, the sterilization of number DX-1~DX-4 EC of the agent composition to wheat powdery mildew50Value is within the scope of 2.73-2.89.As it can be seen that number X-1~X-7 and number DX-1~DX- EC of 4 microbicide compositions to wheat powdery mildew50Value is below biphenyl pyrrole bacterium amine single dose and dimoxystrobin single dose.Further, EC of the microbicide compositions of number X-1~X-7 to wheat powdery mildew50Value is not only significantly less than biphenyl pyrrole bacterium amine single dose and ether bacterium The single dose of amine is also significantly less than contrast sample, the i.e. microbicide compositions of number DX-1~DX-4.
Co-toxicity coefficient the result shows that, biphenyl pyrrole bacterium amine, dimoxystrobin in (30:1)~(1:30) ratio compound to white wheat The co-toxicity coefficient of powder disease 125 or more, has synergistic effect；Especially when biphenyl pyrrole bacterium amine and dimoxystrobin proportion are 10:1, Co-toxicity coefficient is up to 171.87, and synergistic effect is most obvious；Followed by the two proportion is 20:1 and 1:1, and co-toxicity coefficient is above 150, and (sample of number DX-1~DX-4) is compounded according to 40:1,35:1,1:35,1:40, co-toxicity coefficient is in 100-120 range It is interior, show as addition.In conclusion the proportion of biphenyl pyrrole bacterium amine, dimoxystrobin according to (30:1)~(1:30), compared to control Matched described in sample DX-1~DX-4, the former toxicity action highlights, show biphenyl pyrrole bacterium amine and dimoxystrobin according to (30:1)~ The ratio compatibility of (1:30) has significant synergies to the prevention and treatment of wheat powdery mildew.
The test method and experimental result of wheat rust toxicity test are directed in Room 5.2
1. measuring method
1) warm indoor pot culture wheat rust diseased plant.
2) to have infected the wheat of wheat rust as test strain, if 1 clear water control, 13 chemicals treatments；13 medicines Agent processing respectively corresponds biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose, the microbicide compositions of number X-1~X-7, DX-1~DX-4 Microbicide compositions, each processing is arranged 6 concentration gradients, is followed successively by 32mg/L, 16mg/L, 8mg/L, 4mg/L, 2mg/L And 1mg/L；4 repetitions are arranged in each concentration gradient.
3) spraying treatment is carried out with Potter spray tower, spouting liquid 450L/ha is control with clear water, each for calculating Virulence equation, EC50 and the co-toxicity coefficient of medicament.
4) after being administered 15d, control efficiency is calculated with disease index, it is for statistical analysis with DPS data processing software, it finds out Virulence regression equation calculates the EC50 of each medicament, calculates co-toxicity coefficient by Sun Yunpei's Method.
5) virulence is carried out to wheat rust by different microbicide compositions and biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose Analysis, the results are shown in Table 6.
Biphenyl pyrrole bacterium amine is with dimoxystrobin different ratio to the Virulent Analysis of wheat rust in 6 suspending agent type fungicide of table
2. interpretation of result
By the data in table 6 it is found that biphenyl pyrrole bacterium amine and dimoxystrobin single dose are to the EC of wheat rust50Value is respectively 3.48mg/L, 3.09mg/L, the EC of the microbicide compositions of number X-1~X-7 to wheat rust50Value is in 2.22-2.57 range It is interior；EC of the microbicide compositions of number DX-1~DX-4 to wheat rust50Value is within the scope of 2.81-2.96；As it can be seen that number X- EC of the microbicide compositions of 1~X-7 and number DX-1~DX-4 to wheat rust50Value is 3.0 hereinafter, be below biphenyl pyrrole The EC of bacterium amine single dose and dimoxystrobin single dose to wheat rust50Value.Further, the microbicide compositions of number X-1~X-7 are to small The EC of wheat rust50Value is not only significantly less than biphenyl pyrrole bacterium amine single dose and dimoxystrobin single dose, is also significantly less than contrast sample, that is, numbers The microbicide compositions of DX-1~DX-4.
Co-toxicity coefficient the result shows that, biphenyl pyrrole bacterium amine, dimoxystrobin according to (30:1)~(1:30) ratio compound to wheat The co-toxicity coefficient of rust 120 or more, has synergistic effect, especially when biphenyl pyrrole bacterium amine and dimoxystrobin proportion are 10:1, Co-toxicity coefficient is up to 155.21, and synergistic effect is most obvious；Followed by the two proportion is 20:1 and 1:1, and co-toxicity coefficient is respectively 142.35 and 139.23；And (sample of number DX-1~DX-4) is compounded according to 40:1,35:1,1:35,1:40, co-toxicity coefficient exists Within the scope of 100-120, addition is shown as.In conclusion the biphenyl pyrrole bacterium amine and dimoxystrobin of different ratio are to wheat rust Consistent with wheat powdery mildew toxicity action trend, biphenyl pyrrole bacterium amine, dimoxystrobin are compared according to the proportion of (30:1)~(1:30) Matched described in DX-1~DX-4, the former toxicity action highlights, show biphenyl pyrrole bacterium amine and dimoxystrobin according to (30:1)~(1: 30) ratio compatibility has significant synergies to the prevention and treatment of wheat rust.
The test method and experimental result of wheat scab toxicity test are directed in Room 5.3
1. measuring method
1) warm indoor pot culture wheat scab diseased plant.
2) using the wheat for having infected wheat scab as test strain, 1 clear water control, 13 chemicals treatments are set；13 A chemicals treatment respectively correspond biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose, the microbicide compositions of number X-1~X-7, DX-1~ 6 concentration gradients are arranged in the microbicide compositions of DX-4, each processing, be followed successively by 32mg/L, 16mg/L, 8mg/L, 4mg/L, 2mg/L and 1mg/L；4 repetitions are arranged in each concentration gradient.
3) spraying treatment is carried out with Potter spray tower, spouting liquid 450L/ha is control with clear water, each for calculating Virulence equation, EC50 and the co-toxicity coefficient of medicament.
4) after being administered 15d, control efficiency is calculated with disease index, it is for statistical analysis with DPS data processing software, it finds out Virulence regression equation calculates the EC50 of each medicament, calculates co-toxicity coefficient by Sun Yunpei's Method.
5) virulence point is carried out to wheat scab by microbicide compositions and biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose Analysis, experimental result are as shown in table 7.
Biphenyl pyrrole bacterium amine is with dimoxystrobin different ratio to the Virulent Analysis of wheat scab in 7 suspending agent type fungicide of table
2. interpretation of result
By the data in table 7 it is found that biphenyl pyrrole bacterium amine and dimoxystrobin single dose are to the EC of wheat scab50Value is respectively EC of the microbicide compositions of 3.71mg/L and 3.40mg/L, number X-1~X-7 to wheat scab50Value is in 2.47-2.77 model In enclosing；EC of the microbicide compositions of number DX-1~DX-4 to wheat scab50Value is between 3.08-3.20；As it can be seen that number EC of the microbicide compositions of X-1~X-7 and number DX-1~DX-4 to wheat scab50Value is below biphenyl pyrrole bacterium amine single dose With dimoxystrobin single dose to the EC of wheat scab50Value.Further, the microbicide compositions of number X-1~X-7 are to gibberella saubinetii The EC of disease50Value is not only significantly less than biphenyl pyrrole bacterium amine single dose and dimoxystrobin single dose, is also significantly less than contrast sample, i.e. number DX-1 The microbicide compositions of~DX-4.
Co-toxicity coefficient the result shows that, biphenyl pyrrole bacterium amine, dimoxystrobin according to (30:1)~(1:30) ratio compound to wheat The co-toxicity coefficient of head blight 120 or more, has synergistic effect, especially when biphenyl pyrrole bacterium amine and dimoxystrobin proportion are 10:1 When, co-toxicity coefficient is up to 149.11, and synergistic effect is most obvious；Followed by the two proportion is 20:1, co-toxicity coefficient 139.85；And And according to 40:1,35:1,1:35,1:40 compound (sample of number DX-1~DX-4), co-toxicity coefficient within the scope of 100-120, Show as addition.Shown in sum up, the biphenyl pyrrole bacterium amine and dimoxystrobin of different ratio become to the toxicity action of wheat scab Gesture is consistent with wheat rust and wheat powdery mildew, and biphenyl pyrrole bacterium amine, dimoxystrobin are compared according to the proportion of (30:1)~(1:30) Matched described in DX-1~DX-4, the former toxicity action highlights, show biphenyl pyrrole bacterium amine and dimoxystrobin according to (30:1)~(1: 30) ratio compatibility has significant synergies to the prevention and treatment of wheat scab.
6 crop field effect experiment of embodiment
Test period: 2018
Test site: Suiping County, Henan Province
For studying object: wheat
Controlling object: wheat rust, wheat powdery mildew and wheat gray mold
Test method: screening illness field is tested, if 1 clear water control, 13 chemicals treatments；13 chemicals treatments Respectively correspond the sterilization of biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose, the microbicide compositions of number X-1~X-7, DX-1~DX-4 3 sprayed doses, respectively 75ga.i./hm is arranged in agent composition, each processing2、100ga.i./hm2And 125ga.i./hm2。 Using foliar spray method, uniformly it is sprayed on wheat leaf blade in spite of illness.Before application, application after 3 days, 7 days, 14 days, using 5 Point sampling survey method, 5 points of the grab sample in test process area carry out severity Scaling according to national field trial relevant criterion, Calculate disease index and preventive effect.Test result is shown in Table 8~table 10.
(1) the biphenyl pyrrole bacterium amine of different ratio and dimoxystrobin prevent and treat experimental result to wheat rust
The sterilization of biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose, the microbicide compositions of number X-1~X-7, DX-1~DX-4 Agent composition is as shown in table 8 to the prevention and treatment experimental result of wheat rust.
Control efficiency of the biphenyl pyrrole bacterium amine and dimoxystrobin of 8 different ratio of table to wheat rust
Table 8 statistics indicate that, in 75ga.i./hm2、100ga.i./hm2And 125ga.i./hm2Under three sprayed doses, with The increase of dosage, each processing be in increase trend to the control efficiency of wheat rust.As can be seen from Table 8, biphenyl Pyrrole bacterium amine, azoxystrobin compound combination are better than biphenyl pyrrole bacterium amine single dose and Fluoxastrobin single dose to the control efficiency of wheat rust, will Biphenyl pyrrole bacterium amine and Fluoxastrobin according to the ratio of (30:1)~(1:30) compound resulting 7 medicaments, and (number X-1~X-7's kills Antimicrobial composition), it is extremely significant to the control efficiency of wheat rust to be higher than with the biphenyl pyrrole bacterium amine single dose and Fluoxastrobin under dosage Single dose, and also conspicuousness is higher than according to the resulting 4 medicaments (number DX-1~DX-4 of 40:1,35:1,1:35,1:40 compounding Microbicide compositions).When sprayed dose reaches 150ga.i./hm2When, after application 7 days, biphenyl pyrrole bacterium amine and dimoxystrobin according to The ratio compound drug of (30:1)~(1:30) to the control efficiency of wheat rust 88% or more, especially when biphenyl pyrrole bacterium Amine: when the weight ratio of dimoxystrobin is 10:1, being up to 93.36% to the control efficiency of wheat rust, extremely significant to be higher than other ratios The compound drug and single dose of example.This shows biphenyl pyrrole bacterium amine and dimoxystrobin according to the ratio compounding gained of (30:1)~(1:30) Medicament has significant synergistic function to wheat rust, and both biphenyl pyrrole bacterium amine and Fluoxastrobin ratio are closer in medicament 10:1 is better to the control efficiency of wheat rust.
(2) the biphenyl pyrrole bacterium amine of different ratio and dimoxystrobin prevent and treat experimental result to wheat powdery mildew
The sterilization of biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose, the microbicide compositions of number X-1~X-7, DX-1~DX-4 Agent composition is as shown in table 9 to the prevention and treatment experimental result of wheat powdery mildew.
The biphenyl pyrrole bacterium amine and dimoxystrobin that table 9 is different ratio are to the control efficiency of wheat powdery mildew
Table 9 statistics indicate that, in 75ga.i./hm2、100ga.i./hm2And 125ga.i./hm2Under three sprayed doses, with The increase of dosage, each processing be in increase trend to the control efficiency of wheat powdery mildew.As can be seen from Table 9, join Benzene pyrrole bacterium amine and Fluoxastrobin are combined by compounding is better than biphenyl pyrrole bacterium amine single dose and Fluoxastrobin to the control efficiency of wheat powdery mildew Single dose, by biphenyl pyrrole bacterium amine and Fluoxastrobin according to the ratio of (30:1)~(1:30) compound resulting 7 medicaments (number X-1~ The microbicide compositions of X-7), it is extremely significant to the control efficiency of wheat powdery mildew to be higher than with the biphenyl pyrrole bacterium amine single dose under dosage With Fluoxastrobin single dose, and also conspicuousness is higher than and compounds resulting 4 medicaments (number DX- according to 40:1,35:1,1:35,1:40 The microbicide compositions of 1~DX-4).When sprayed dose is 150ga.i./hm2When, after application 7 days, biphenyl pyrrole bacterium amine and ether bacterium Amine is above 90% to the control efficiency of wheat powdery mildew according to the ratio compound drug of (30:1)~(1:30), especially works as biphenyl When the weight ratio of pyrrole bacterium amine and Fluoxastrobin is 10:1,94.04% is up to the control efficiency of wheat powdery mildew, it is extremely significant to be higher than The compound drug and single dose of other ratios.This shows that biphenyl pyrrole bacterium amine and dimoxystrobin are multiple according to the ratio of (30:1)~(1:30) It is consistent with wheat rust with control efficiency of the gained medicament to wheat powdery mildew, there is significant synergistic function, and And both biphenyl pyrrole bacterium amine in medicament and Fluoxastrobin ratio are closer to 10:1, it is better to the control efficiency of wheat powdery mildew.
(3) the biphenyl pyrrole bacterium amine of different ratio and dimoxystrobin prevent and treat experimental result to wheat scab
The sterilization of biphenyl pyrrole bacterium amine single dose, dimoxystrobin single dose, the microbicide compositions of number X-1~X-7, DX-1~DX-4 Agent composition is as shown in table 10 to the prevention and treatment experimental result of wheat scab.
The biphenyl pyrrole bacterium amine and dimoxystrobin that table 10 is different ratio are to the control efficiency of wheat scab
Table 10 statistics indicate that, in 75ga.i./hm2、100ga.i./hm2And 125ga.i./hm2Under three sprayed doses, with The increase of dosage, each processing is also in increase trend to the control efficiency of wheat scab.As can be seen from Table 10, Biphenyl pyrrole bacterium amine, azoxystrobin compound combination are better than biphenyl pyrrole bacterium amine single dose and Fluoxastrobin list to the control efficiency of wheat scab Biphenyl pyrrole bacterium amine and dimoxystrobin are compounded resulting 7 medicaments (number X-1~X-7 according to the ratio of (30:1)~(1:30) by agent Microbicide compositions), to the control efficiency of wheat scab it is extremely significant be higher than under dosage biphenyl pyrrole bacterium amine single dose and Fluoxastrobin single dose, and also conspicuousness is higher than according to resulting 4 medicaments (the number DX-1 of 40:1,35:1,1:35,1:40 compounding The microbicide compositions of~DX-4).When sprayed dose is 150ga.i./hm2When, after application 7 days, biphenyl pyrrole bacterium amine and dimoxystrobin According to the medicament that (30:1)~(1:30) ratio compounds, 87% is above to the control efficiency of wheat scab, especially works as biphenyl When the weight ratio of pyrrole bacterium amine and Fluoxastrobin is 10:1,91.57% is up to the control efficiency of wheat scab, followed by works as connection When the weight ratio of benzene pyrrole bacterium amine and Fluoxastrobin is 20:1,90.04% is reached to the control efficiency of wheat scab, extremely significant height In the compound drug and single dose of other ratios.This shows biphenyl pyrrole bacterium amine and dimoxystrobin according to the ratio of (30:1)~(1:30) Compounding gained medicament is consistent with wheat rust, wheat powdery mildew to the control efficiency of wheat scab, has significant collaboration Synergistic effect, and prevention and treatment of the ratio closer to 10:1, to wheat scab of both biphenyl pyrrole bacterium amine in medicament and Fluoxastrobin Effects are better.
The comprehensive interpretation of result to 8~table of table 10, when biphenyl pyrrole bacterium amine and dimoxystrobin are according to the ratio of (30:1)~(1:30) Example compounds resulting medicament, is significantly better than biphenyl pyrrole bacterium to the control efficiency of wheat scab, wheat rust and wheat powdery mildew Four medicaments that the single dose and the two of amine and Fluoxastrobin are compounded according to 40:1,35:1,1:35 and 1:40 illustrate biphenyl pyrrole bacterium amine Resulting microbicide compositions are compounded according to the ratio of (30:1)~(1:30) with dimoxystrobin, to wheat scab, wheat rust Significant synergistic function is all had with wheat powdery mildew, to wheats such as wheat rust, wheat powdery mildew and wheat scabs The control efficiency of fungal disease is excellent, is worth carrying out promotion and application in modern agriculture.

Claims (10)

1. a kind of microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin, which is characterized in that the microbicide compositions have Ingredient is imitated as biphenyl pyrrole bacterium amine and dimoxystrobin, the weight ratio of biphenyl pyrrole bacterium amine and dimoxystrobin is (30:1)~(1:30).
2. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 1, which is characterized in that described The weight ratio of benzene pyrrole bacterium amine and dimoxystrobin is (20:1)~(1:10).
3. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 2, which is characterized in that described Benzene pyrrole bacterium amine and dimoxystrobin total weight ratio shared in gross weight in the microbicide compositions are 10%~80%.
4. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 3, which is characterized in that described to kill Antimicrobial composition is any one in suspending agent, missible oil, wettable powder and water dispersible granules.
5. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 4, which is characterized in that described to kill It further include auxiliary agent in antimicrobial composition；The auxiliary agent is wetting agent, dispersing agent, filler, emulsifier, thickener, disintegrating agent, bonding One of agent, antifreezing agent and solvent are a variety of.
6. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 5, which is characterized in that described to kill Antimicrobial composition is suspending agent type, weight composition are as follows: the sum of biphenyl pyrrole bacterium amine and dimoxystrobin weight are 10~50%, emulsifier 3 ~8%, thickener 0.5~2%, antifreezing agent 3%~8%, dispersing agent 3%~5%, water complements to 100%.
7. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 5, which is characterized in that described to kill Antimicrobial composition is missible oil type, weight composition are as follows: the sum of biphenyl pyrrole bacterium amine and dimoxystrobin weight are 10%~40%, emulsifier 9% ~15%, solvent complements to 100%.
8. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 5, which is characterized in that described to kill Antimicrobial composition is water dispersible granules, weight composition are as follows: the sum of biphenyl pyrrole bacterium amine and dimoxystrobin weight are 10%~80%, wetting Agent 3~6%, dispersing agent 3~5%, disintegrating agent 2~6%, binder 0.5~3%, filler complements to 100%.
9. the microbicide compositions of pyrrole containing biphenyl bacterium amine and dimoxystrobin according to claim 5, which is characterized in that described to kill Antimicrobial composition is wettable powder, weight composition are as follows: the sum of biphenyl pyrrole bacterium amine and dimoxystrobin weight are 10%~60%, wetting Agent 5~15%, dispersing agent 3%~15%, filler complements to 100%.
10. the fungicide of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin as described in claim 1~9 is any prevention and treatment wheat powdery mildew, Application in rust and head blight.
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CN103907605A (en) * 2012-12-31 2014-07-09 江苏丰登农药有限公司 Composite synergic sterilization composition containing boscalid and bixafen and application thereof

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WO2012019981A1 (en) * 2010-08-09 2012-02-16 Basf Se Fungicidal mixtures
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CN103907605A (en) * 2012-12-31 2014-07-09 江苏丰登农药有限公司 Composite synergic sterilization composition containing boscalid and bixafen and application thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116439245A (en) * 2023-04-14 2023-07-18 华南农业大学 Bactericide with safety and low toxicity to aquatic organisms and application thereof
CN116439245B (en) * 2023-04-14 2024-01-02 华南农业大学 Bactericide with safety and low toxicity to aquatic organisms and application thereof

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