CN116439245A - Bactericide with safety and low toxicity to aquatic organisms and application thereof - Google Patents

Bactericide with safety and low toxicity to aquatic organisms and application thereof Download PDF

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Publication number
CN116439245A
CN116439245A CN202310398436.XA CN202310398436A CN116439245A CN 116439245 A CN116439245 A CN 116439245A CN 202310398436 A CN202310398436 A CN 202310398436A CN 116439245 A CN116439245 A CN 116439245A
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bactericide
bixafen
concentration
fish
zebra fish
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CN116439245B (en
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曾泰儒
李文华
高博
王偲
王胜坤
梁晓晗
彭友贵
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South China Agricultural University
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South China Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
    • A01N47/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom containing >N—S—C≡(Hal)3 groups
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Dispersion Chemistry (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)

Abstract

The invention discloses a bactericide which is safe and low in toxicity to aquatic organisms and application thereof. Belongs to the technical field of chemical bacteriostats. In agricultural modes such as wetland ecosystem and rice and fish co-cultivation, on the premise of not affecting normal growth and development of fish embryos, the growth of plant disease microorganisms can be inhibited, the bixafen and the benzofomesafen are compounded according to the concentration ratio of 3:1, the obvious inhibition effect on plant diseases is shown, and the antibacterial composition is efficient, safe to aquatic organisms and has good market application prospect.

Description

Bactericide with safety and low toxicity to aquatic organisms and application thereof
Technical Field
The invention relates to the technical field of chemical bacteriostats, in particular to a bactericide which is safe and low in toxicity to aquatic organisms and application thereof.
Background
The wetland is one of the most important ecological systems in the world, is self-known as the kidney of the earth, has unique functions, can provide food and production raw materials for human beings, and has irreplaceable functions in the aspects of regulating climate, regulating flood, purifying pollution, maintaining biological diversity and maintaining ecological balance. The paddy field is a part of a wetland system, meanwhile, the paddy field is one of main grain crops in the world, the meaning of the grain safety guarantee of human beings is self-evident, the comprehensive planting and breeding of the paddy field and the fish combined with the fish can concentrate two resource advantages of the paddy field and the aquatic products, the paddy field is used for breeding the fish, and the fish is used for promoting the paddy field, so that the paddy field is a high-efficiency ecological fishery mode. However, in some cases, wetlands such as paddy fields are susceptible to diseases such as rice blast, which not only easily affect the economic output in such areas, but also cause damage to the ecological environment.
In order to improve the above conditions, a variety of means are used by human beings to control plant diseases which are harmful to the health of the wetland ecosystem, wherein chemical reagents are a common and efficient method, however, most of the reagents are not ideal in single use effect, are easy to cause drug resistance of target objects, and are easy to have adverse effects on the recycling of soil or the growth and development of plants after long-term use, and certain reagents are also diffused into the water body of the wetland system to affect the growth and development of aquatic organisms.
The search for a medicament which is safe to aquatic organisms and can efficiently inhibit bacteria is a feasible solution, meanwhile, the drug resistance of a target object can be reduced by compounding a plurality of medicaments, but the combined use of different bacteriostats can produce synergistic or antagonistic action, so that the compounding effect of the different bacteriostats needs to be verified.
In summary, how to provide a medicament which is safe to aquatic organisms and can inhibit bacteria efficiently is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides a bactericide which is safe and low in toxicity to aquatic organisms and application thereof.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a bacteriostatic composition with safety and low toxicity to aquatic organisms consists of bixafen and benzene sulfonamide, wherein the concentration ratio of the bixafen to the benzene sulfonamide is (1-3) (1-2).
Further, the concentration ratio of bixafen to benfomesafen is 1:2.
Further, the concentration ratio of bixafen to benfomesafen is 3:2.
Further, the concentration ratio of bixafen to benfomesafen is 3:1.
A bactericide which is safe and low in toxicity to aquatic organisms comprises the antibacterial composition.
Further, the concentration of the antibacterial composition in the bactericide is 60-160 ppm.
Preferably, the concentration of the bacteriostatic composition in the bactericide is 160ppm.
Further, the concentration of bixafen in the bactericide is 20-120 ppm, and the concentration of the benzofuranone in the bactericide is 40ppm.
Preferably, the concentration of bixafen in the bactericide is 120ppm and the concentration of the benzofomesalamine in the bactericide is 40ppm.
Further, the bactericide is a liquid preparation;
preferably, the bactericide is a suspending agent.
Further, the bactericide also comprises pharmaceutically acceptable auxiliary materials.
Further, the auxiliary material is one or more of dispersing agent, stabilizer, filler and solvent.
The bactericide is applied to the prevention and treatment of plant diseases.
Further, the plant disease is rice blast.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a bacteriostatic composition for inhibiting the growth of rice blast fungi, which can be used for preventing diseases, reducing the harm to a wetland ecosystem, and having no obvious influence on the growth and development of fish embryos, and has wide application prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a diagram showing the development of the embryo of zebra fish.
Figure 2 is a graph of zebra fish body length, where different lower case letters indicate significant differences (P < 0.05).
Figure 3 is a graph showing the inhibition of Pyricularia oryzae wherein the different lower case letters indicate significant differences (P < 0.05).
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The required medicament is a conventional experimental medicament and is purchased from a commercial channel; the test methods not mentioned are conventional test methods and will not be described in detail herein.
The zebra fish is a model species and has the characteristics of easy breeding, rapid growth and development, rapid propagation, transparent fertilized eggs, easy observation and the like, so the following experimental subjects select the zebra fish.
Example 1
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Collecting embryo under natural spawning condition, and adding bixafen to embryo one day after fertilization with concentration of 0.1 μm (concentration of bixafen in water after addition); the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. The body length is measured at 48 and 72 hours, and the fish embryo development is observed by photographing, if the curl is regarded as malformation (a plurality of papers take the malformation as a judgment standard, the zebra fish embryo which is normally developed is linear, and only the stress reaction or abnormal development can be bent, such as https:// doi.org/10.1016/j.chemosphere.2020.128781), and the method is repeated for 10 times.
Example 2
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Collecting embryo under natural spawning condition, and adding bixafen to embryo one day after fertilization with concentration of 0.3 μm (concentration of bixafen in water after addition); the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. Body length was measured at 48 and 72 hours and photographed to see if the fish embryo development was abnormal, and if the curl was considered abnormal, repeated 10 times.
Example 3
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Collecting embryo under natural spawning condition, and adding bixafen to embryo one day after fertilization with concentration of 0.6 μm (concentration of bixafen in water after addition); the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. Body length was measured at 48 and 72 hours and photographed to see if the fish embryo development was abnormal, and if the curl was considered abnormal, repeated 10 times.
Example 4
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Collecting embryo under natural spawning condition, and adding benzene sulfonamide to embryo one day after fertilization with concentration of 0.2 μm (concentration of benzene sulfonamide in water after addition); the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. Body length was measured at 48 and 72 hours and photographed to see if the fish embryo development was abnormal, and if the curl was considered abnormal, repeated 10 times.
Example 5
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Collecting embryo under natural spawning condition, and adding bixafen and benfomesafen to the embryo one day after fertilization, wherein the concentration ratio is 1:2, and the concentration ratio is 0.1 mu M and 0.2 mu M respectively (the concentration of bixafen and benfomesafen in water after addition); the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. Body length was measured at 48 and 72 hours and photographed to see if the fish embryo development was abnormal, and if the curl was considered abnormal, repeated 10 times.
Example 6
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Collecting embryo under natural spawning condition, adding bixafen and benfomesafen to the embryo one day after fertilization, wherein the concentration ratio is 3:2, and the concentrations are 0.3 mu M and 0.2 mu M (the concentrations of bixafen and benfomesafen in water after addition); the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. Body length was measured at 48 and 72 hours and photographed to see if the fish embryo development was abnormal, and if the curl was considered abnormal, repeated 10 times.
Example 7
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Collecting embryo under natural spawning condition, adding bixafen and benfomesafen to the embryo one day after fertilization, wherein the concentration ratio is 3:1, and the concentrations are respectively 0.6 mu M and 0.2 mu M (the concentrations of bixafen and benfomesafen in water after addition); the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. Body length was measured at 48 and 72 hours and photographed to see if the fish embryo development was abnormal, and if the curl was considered abnormal, repeated 10 times.
Comparative example 1
Zebra fish were fed at 26.5 ℃,14h light cycle and 10h dark cycle. Embryos are collected under natural spawning conditions and sterile water equivalent to example 1 is added to the embryos one day after fertilization; the lowest end of the tail of the zebra fish is taken as a starting point, the vertical point from the top of the zebra fish to the ridge line is taken as an ending point along the linear direction of the ridge, and the distance from the starting point to the ending point is recorded as the length of the zebra fish. Body length was measured at 48 and 72 hours and photographed to see if the fish embryo development was abnormal, and if the curl was considered abnormal, repeated 10 times.
Example 8
Inoculating Pyricularia oryzae into potato agarose (PDA) culture medium, culturing at 28deg.C for 3-5 d, collecting spores to sterile 0.9% NaCl solution, filtering with absorbent cotton, and counting until the concentration reaches 10 10 CFU/mL, diluted for use.
Example 9
A single bacteriostatic agent comprising bixafen added to a PDB medium at a concentration of 2mg/L in the medium after the addition, and adding the spore suspension obtained in example 8 to a concentration of 10 7 CFU/mL, repeated 5 times.
Example 10
A single bacteriostat comprising bixafen and the same volume of the bacteriostat of example 9, added into PDB culture solution, the bixafen concentration in the culture medium after the addition is 6mg/L, and the spore suspension prepared in example 8 is added to make the concentration 10 7 CFU/mL, repeated 5 times.
Example 11
A single bacteriostat comprising bixafen and the same volume of the bacteriostat of example 9, added into PDB culture solution, the bixafen concentration in the culture medium after the addition is 12mg/L, and the spore suspension prepared in example 8 is added to make the concentration 10 7 CFU/mL, repeated 5 times.
Example 12
A single antibacterial agent, which is prepared from the components of the antibacterial agent,it comprises adding the same volume of solution of benzene sulfonamide and the bacteriostat of example 9 into PDB culture solution, adding the concentration of benzene sulfonamide in the culture medium after adding to 4mg/L, and adding the spore suspension prepared in example 8 to make the concentration 10 7 CFU/mL, repeated 5 times.
Example 13
A bacteriostatic composition comprising bixafen and benzofomesalamine. The concentration ratio of the benzene sulfonamide to the bixafen is 1:2, the concentration ratio of the benzene sulfonamide to the bixafen is equal to the volume of the solution of the bacteriostat of the example 9, the solution is added into Potato Dextrose Broth (PDB) culture solution, the concentration of the added solution in the culture medium is 2mg/L and 4mg/L respectively, and the spore suspension prepared in the example 8 is added to make the concentration be 10 7 CFU/mL, repeated 5 times.
Example 14
A bacteriostatic composition comprising bixafen and benzofomesalamine. The concentration ratio of bixafen to benfomesafen is 3:2, the bixafen and the benfomesafen are added into Potato Dextrose Broth (PDB) culture solution with the same volume as the bacteriostatic agent of the example 9, the concentration in the culture medium after the bixafen and the benfomesafen are respectively 6mg/L and 4mg/L, and the spore suspension prepared in the example 8 is added to obtain the spore suspension with the concentration of 10 7 CFU/mL, repeated 5 times.
Example 15
A bacteriostatic composition comprising bixafen and benzofomesalamine. The concentration ratio of bixafen to benfomesafen is 3:1, the bixafen and the benfomesafen are equal to the volume of the solution of the bacteriostat in example 9, the bixafen and the benfomesafen are added into Potato Dextrose Broth (PDB) culture solution, the concentration in the culture medium after the bixafen and the benfomesafen are respectively 12mg/L and 4mg/L, and the spore suspension prepared in example 8 is added so that the concentration is 10 7 CFU/mL, repeated 5 times.
Comparative example 2
Deionized sterile water, the same volume as the bacteriostat of example 9, was added to the PDB broth, and the spore suspension prepared in example 8 was added to give a concentration of 10 7 CFU/mL, repeated 5 times.
Comparative example 3
The PDB broth was repeated 5 times with the same total solution volume as in example 9.
Toxicity test results
The zebra fish body lengths and fish embryo development pictures of examples 1 to 7 and comparative example 1 are shown in fig. 1 and 2.
As can be seen from fig. 1, in example 3, embryo development is obviously malformed compared with other treatments, while in example 7, the concentration of bixafen is equal to that of example 3, but after the benzene sulfonamide is added, the influence on the embryo development of fish is obviously reduced, and thus, the toxicity on the embryo development of fish can be reduced by compounding the two medicaments.
As can be seen from fig. 2, the zebra fish of examples 4, 5 and 6 had the highest body length value (exceeding comparative example 1) at 48h, wherein the body length values of examples 5 and 6 were relatively higher, and the body length values of examples 5, 6 and 7 were all higher than those of examples 1, 2 and 3 containing the same concentration of bixafen, respectively, which demonstrated that the addition of the benzofuranone not only reduced the toxicity of bixafen to fish, but also promoted the growth of the prophase fish embryo. At 72h, there was no significant difference in zebra fish length values between examples 5 and 6 and examples 1 and 2, which contained equal concentrations of bixafen, while example 7 was significantly higher than example 3, indicating that this concentration ratio (bixafen to benfomesafen concentration ratio of 3:1) was effective in reducing the negative effects of bixafen on fish embryo development. In summary, example 7 is the preferred embodiment.
Bacteriostasis test
The culture solutions of examples 9 to 15 and comparative examples 2 to 3 were incubated at 28℃for 24 hours, absorbance at 600nm was measured, and the antibacterial ratio was calculated. The calculation formula of the bacteriostasis rate is as follows:
antibacterial ratio= (OD value of control 2-OD value of example)/(OD value of control 2-OD value of control 3) ×100%, and the result is shown in fig. 3.
As can be seen from fig. 3, the bacteriostasis rates of example 14 and example 15 are the highest at 24h, wherein the bacteriostasis effect of example 15 is relatively better, and all the compounding treatments are better than the treatment of the bixafen added alone, so that the bacteriostasis capability of the two chemical agents is increased in total. And combining the test results of zebra fish (the best effect of the embodiment 7), and meanwhile, the compound proportion of the two medicaments of the embodiment 7 and the embodiment 15 is the same, so that the compound proportion of the bixafen and the benfomesafen is regarded as the best compound proportion when the compound proportion is 3:1.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (10)

1. The antibacterial composition is characterized by comprising bixafen and benzene-fluoro sulfanilamide, wherein the concentration ratio of the bixafen to the benzene-fluoro sulfanilamide is (1-3) to (1-2).
2. A bacteriostatic composition according to claim 1 wherein the concentration ratio of bixafen to benfomesafen is 3:1.
3. A bactericide which is safe and low in toxicity to aquatic organisms, characterized by comprising the bacteriostatic composition according to any one of claims 1 or 2.
4. A biocide as claimed in claim 3 wherein the concentration of the bacteriostatic composition in the biocide is 60 to 160ppm;
preferably, the concentration of the bacteriostatic composition in the bactericide is 160ppm.
5. The bactericide according to claim 4, characterized in that the concentration of bixafen in the bactericide is 20 to 120ppm and the concentration of benzofomesalamine in the bactericide is 40ppm;
preferably, the concentration of the bixafen in the bactericide is 120ppm, and the concentration of the benzofomesalamine in the bactericide is 40ppm.
6. A sterilant according to claim 3 wherein said sterilant is a liquid formulation;
preferably, the bactericide is a suspending agent.
7. A sterilant according to claim 3, wherein said sterilant further comprises a pharmaceutically acceptable adjuvant.
8. The bactericide according to claim 7, characterized in that the auxiliary material is one or more of a dispersant, a stabilizer, a filler and a solvent.
9. Use of the bactericide according to any one of claims 3 to 8 for controlling plant diseases.
10. The use according to claim 9, wherein the plant disease is rice blast.
CN202310398436.XA 2023-04-14 2023-04-14 Bactericide with safety and low toxicity to aquatic organisms and application thereof Active CN116439245B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106719720A (en) * 2017-02-14 2017-05-31 佛山市盈辉作物科学有限公司 A kind of herbicidal composition containing bentazone
CN109006819A (en) * 2018-09-25 2018-12-18 广东广康生化科技股份有限公司 A kind of bactericidal composition and application thereof containing zoxamide Yu biphenyl pyrrole bacterium amine
CN110402937A (en) * 2019-08-19 2019-11-05 河南省农业科学院植物保护研究所 A kind of microbicide compositions and its application of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106719720A (en) * 2017-02-14 2017-05-31 佛山市盈辉作物科学有限公司 A kind of herbicidal composition containing bentazone
CN109006819A (en) * 2018-09-25 2018-12-18 广东广康生化科技股份有限公司 A kind of bactericidal composition and application thereof containing zoxamide Yu biphenyl pyrrole bacterium amine
CN110402937A (en) * 2019-08-19 2019-11-05 河南省农业科学院植物保护研究所 A kind of microbicide compositions and its application of the bacterium amine of pyrrole containing biphenyl and dimoxystrobin

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