CN110402937B - Bactericide composition containing bixafen and dimoxystrobin and application thereof - Google Patents
Bactericide composition containing bixafen and dimoxystrobin and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a composition of a bactericide containing bixafen and dimoxystrobin. The active ingredients of the bactericide composition are bixafen and dimoxystrobin, and the weight ratio of the bixafen to the dimoxystrobin is (30:1) - (1: 30). The bactericide Bixafen and dimoxystrobin which have different action mechanisms are mixed for use, so that the bactericide has obvious synergistic effect on wheat powdery mildew, wheat rust and wheat scab, and pathogenic microorganisms are effectively controlled to generate drug resistance; the field test shows that when the ratio of the bixafen to the dimoxystrobin is 10:1, after the pesticide composition is used for 7 days, the control effect on wheat rust is as high as 93.36%, the control effect on wheat powdery mildew is as high as 94.04%, the control effect on wheat scab is as high as 91.57%, and the control effects are obviously higher than that of a bixafen single agent and that of a dimoxystrobin single agent.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition containing bixafen and dimoxystrobin and application thereof.
Background
Bixafen (Bixafen), its chemical formula is C18H12Cl2F3N3O, its chemical name is N- (3 ', 4' -dichloro-5-fluorobiphenyl-2-yl) -3- (difluoromethyl) -1-methylpyrazole-4-carboxamide. Bixafen is a pyrazole bactericide, and is a novel systemic bactericide with broad spectrum and combined protection and treatment activities. Bixafen inhibits the functions of mitochondria, prevents the generation of energy, inhibits the growth of pathogenic bacteria and finally causes the death of the pathogenic bacteria by interfering succinate dehydrogenase on a complex II in a respiratory electron transfer chain of the pathogenic bacteria mitochondria. Bixafen is a systemic fungicide, has a wide bactericidal spectrum and is specially used for foliar spray. Experiments prove that bixafen has excellent control effect on various diseases of wheat crops, such as wheat leaf blight, leaf rust, stripe rust, powdery mildew, scab and the like, and can control leaf spot disease and the like caused by septoria pathogenic bacteria which generate resistance to strobilurin fungicides. Bixafen is reported to have good treatment and protection effects on barley net blotch and apple powdery mildew.
Dimoxystrobin (Dimoxystrobin) with molecular formula of C19H22N2O3 and chemical name of (E) -2- (methoxyimino) -N-methyl-2 [ a- (2, 5-dimethylphenoxy) -O-tolyl ] acetamide. Ether mycoamine is a quinone external inhibitor, bonds with oxidation sites of reduced coenzyme Q of cytochrome B, thereby inhibits the respiration of mitochondria, and also becomes a Q0 inhibitor, thereby interfering the energy circulation in the fungus body, having protectiveness, therapeutic property and conductivity, and inhibiting spore germination and hypha growth.
Wheat rust, powdery mildew and scab are main diseases in the middle and later periods of wheat production, the killing effect of a single bactericide on plant diseases and insect pests is limited, and after the single bactericide is used for a period of time, pathogenic microorganisms are easily led to generate drug resistance, and the effect of killing the plant diseases and insect pests is reduced.
Disclosure of Invention
The invention aims to provide a bactericide composition containing bixafen and dimoxystrobin and application thereof, which are used for improving the killing effect on wheat powdery mildew, rust disease and scab.
Based on the purpose, the invention adopts the following technical scheme: a bactericide composition containing bixafen and dimoxystrobin comprises the effective components of the bixafen and the dimoxystrobin in a weight ratio of (30:1) - (1: 30).
Further, the weight ratio of bixafen to dimoxystrobin is (20:1) - (1: 10).
Further, the total weight of bixafen and dimoxystrobin accounts for 10% -80% of the total weight of the bactericide composition.
Further, the bactericide composition is any one of a suspending agent, missible oil, wettable powder and water dispersible granules.
Furthermore, the bactericide composition also comprises an auxiliary agent; the auxiliary agent is one or more of wetting agent, dispersing agent, filler, emulsifier, thickening agent, disintegrating agent, binder, antifreezing agent and solvent.
Further, the bactericide composition is in a suspension form, and comprises the following components in parts by weight: the total weight of bixafen and dimoxystrobin is 10-50%, the emulsifier is 3-8%, the thickener is 0.5-2%, the antifreeze is 3-8%, the dispersant is 3-5%, and the water is added to 100%.
Further, the bactericide composition is an emulsifiable concentrate type, and comprises the following components in parts by weight: the total weight of bixafen and dimoxystrobin is 10-40%, the emulsifier is 9-15%, and the solvent is complemented to 100%.
Further, the bactericide composition is water dispersible granules, and comprises the following components in parts by weight: the total weight of bixafen and dimoxystrobin is 10-80%, the wetting agent is 3-6%, the dispersing agent is 3-5%, the disintegrating agent is 2-6%, the binder is 0.5-3%, and the filler is added to 100%.
Further, the bactericide composition is wettable powder, and comprises the following components in parts by weight: the total weight of bixafen and dimoxystrobin is 10-60%, the wetting agent is 5-15%, the dispersant is 3-15%, and the filler is added to 100%.
Further, the wetting agent is any one or more of sodium lauryl sulfate, nekal BX, sodium dodecyl sulfate and sodium dodecyl benzene sulfonate; the dispersant is any one or more of methylene dinaphthalene sodium sulfonate, lignin calcium carbonate, alkyl naphthalene sulfonate and alkylphenol polyoxyethylene; the filler is one or more of white carbon black, kaolin, bentonite and diatomite; the emulsifier is any one or more of phenethyl phenol polyoxyethylene ether, fatty alcohol block polyether, NP-7 and agricultural emulsion series; the thickener is any one or more of gelatin, xanthan gum, hydroxymethyl cellulose, hydroxyethyl cellulose, polyvinylpyrrolidone and polyvinyl acetate; the disintegrating agent is one or more of sodium chloride, urea, ammonium sulfate and sodium carbonate; the binder is any one or more of polyvinyl alcohol, polyethylene glycol and soluble starch; the antifreezing agent is any one or more of ethylene glycol, propylene glycol and glycerol; the solvent is any one or more of solvent naphtha, pyrrolidone, NN dimethylformamide or methyl oleate.
The bactericide containing bixafen and dimoxystrobin is applied to preventing and treating wheat powdery mildew, rust disease and gibberellic disease.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, Bixafen and dimoxystrobin are compounded and combined, so that the composition has an obvious synergistic effect on wheat powdery mildew, wheat rust and wheat scab; particularly, when the ratio of the bixafen to the dimoxystrobin is 10:1, the co-toxicity coefficient for wheat powdery mildew is up to 171.87, the co-toxicity coefficient for wheat rust is up to 155.21, and the co-toxicity coefficient for wheat scab is up to 149.11, so that the mixing of the bixafen and the dimoxystrobin according to the ratio of 10:1 has a more remarkable synergistic effect on the wheat powdery mildew, the wheat rust and the wheat scab.
2. The field test shows that when the weight ratio of the bixafen to the dimoxystrobin is 10:1, after the bactericidal composition is used for 7 days, the control effect on wheat rust is 93.36 percent, the control effect on wheat powdery mildew is 94.04 percent, the control effect on wheat scab is 91.57 percent, and the control effects are obviously higher than that of a bixafen single agent and that of a dimoxystrobin single agent.
3. Bixafen inhibits the functions of mitochondria, prevents the mitochondria from generating energy, inhibits the growth of the pathogens and finally causes the pathogens to die by interfering succinate dehydrogenase on a complex II in the respiratory electron transfer chain of the pathogens; the dimoxystrobin is a quinone external inhibitor and is bonded with an oxidation site of reduced coenzyme Q of cytochrome B so as to inhibit the respiration of mitochondria, and is also called as a Q0 inhibitor to interfere with the energy circulation in fungi so as to inhibit the spore germination and hypha growth; the invention adopts the compounding and combination of bixafen and dimoxystrobin which have different action mechanisms on pathogenic microorganisms, and can realize multi-target site action on the pathogenic microorganisms, thereby effectively controlling the pathogenic microorganisms to generate drug resistance.
Detailed Description
EXAMPLE 1 Bactericide composition containing Bixafen and Etheramine as suspending agent
According to the table 1, water, an emulsifier, a dispersant and an antifreeze are added into a mixing kettle and stirred, the materials are uniformly mixed, bixafen and dimoxystrobin are added into the mixing kettle and stirred uniformly, then the materials are sheared, sanded and uniformly mixed with a thickening agent, and a series of suspension type bactericide compositions containing the bixafen and the dimoxystrobin are prepared, wherein the numbers of the suspension type bactericide compositions are X-1 to X-7 respectively.
Wherein A is the abbreviation of bixafen; b is the abbreviation of dimoxystrobin; b represents the weight ratio of bixafen to dimoxystrobin; (A + B)% represents the percentage of the sum of the weight of bixafen and the weight of dimoxystrobin in the total weight of the bactericide composition. A, B in other embodiments has the same meaning as above.
Meanwhile, the control samples DX-1 to DX-4 are prepared according to the method for subsequent tests.
TABLE 1 concrete formulation of fungicide compositions in different suspension forms
EXAMPLE 2 emulsion-type Fungicide composition containing Bixafen and Etheramine
According to the table 2, NN dimethylformamide and agricultural emulsion 1602 are put into a mixing kettle and are stirred uniformly, and bixafen and dimoxystrobin are put into the mixing kettle and are stirred uniformly to form a uniform and transparent liquid, so that a series of emulsifiable concentrate type bactericide compositions containing the bixafen and the dimoxystrobin are prepared, and the numbers of the emulsifiable concentrate type bactericide compositions are R-1-R-7 respectively.
TABLE 2 concrete formulation of different emulsion type bactericide composition
Example 3 Bactericide composition containing Bixafen and dimoxystrobin in Water dispersible granule
According to the table 3, bixafen, dimoxystrobin and filler are uniformly mixed, crushed by a jet mill, mixed with a wetting agent, a dispersing agent, a disintegrating agent and a binder, added with water according to 10% of the total weight of the mixture, uniformly mixed, extruded, granulated, dried, sieved and the like to prepare a series of water dispersible granule bactericide compositions containing bixafen and dimoxystrobin, which are respectively numbered from S-1 to S-7.
TABLE 3 concrete formulation of fungicide compositions in different water dispersible granule forms
Note: the granulation water was added separately in an amount of 10% by weight based on the total weight of the formulation in Table 3, and was removed by drying after granulation, so that it was not shown in Table 3.
EXAMPLE 4 Bactericide composition containing Bixafen and dimoxystrobin as wettable powder
According to the table 4, bixafen, dimoxystrobin, a wetting agent, a dispersing agent and a filler are uniformly mixed and crushed by a jet mill to prepare a series of wettable powder bactericide compositions containing the bixafen and the dimoxystrobin, which are respectively numbered from S-1 to S-7.
TABLE 4 concrete formulation of fungicide compositions in different wettable powder forms
Example 5 indoor virulence determination assay
5.1 indoor test method for determining toxicity of wheat powdery mildew and test result
As indexes such as a toxicity equation, EC50, a co-toxicity coefficient and the like in the toxicity determination test result are mainly related to effective components of the pesticide and the compounding ratio of the effective components, and are less influenced by the pesticide dosage form, the invention selects the suspension dosage form bactericide composition as a typical dosage form to discuss whether the compounding of the effective components has a synergistic effect.
1. Measurement method
1) And cultivating wheat powdery mildew disease plants in pots in a greenhouse.
2) Wheat infected with wheat powdery mildew is taken as a test strain, 1 clear water control is set, and 13 medicaments are used for treatment; the 13 medicament treatments respectively correspond to bixafen single dose, dimoxystrobin single dose, bactericide compositions with numbers of X-1-X-7 and bactericide compositions with numbers of DX-1-DX-4, and each treatment is provided with 6 concentration gradients which are 32mg/L, 16mg/L, 8mg/L, 4mg/L, 2mg/L and 1mg/L in sequence; each concentration gradient setup was repeated 4 times.
3) And (3) carrying out spraying treatment by using a Potter spraying tower, wherein the spraying liquid amount is 450L/ha, and clear water is used as a reference for calculating a toxicity equation, EC50 and a co-toxicity coefficient of each medicament.
4) After 15 days of drug application, the prevention and treatment effect is calculated according to the disease index, the DPS data processing software is used for statistical analysis to work out a toxicity regression equation, the EC50 of each drug is calculated, and the cotoxicity coefficient is calculated according to the Sun cloud Peel method.
5) Toxicity analysis is carried out on wheat powdery mildew through the bactericide composition, the bixafen single agent and the dimoxystrobin single agent, and the results are shown in table 5.
TABLE 5 analysis of the virulence of wheat powdery mildew of Bixafen and dimoxystrobin in different ratios in the suspension-type fungicide
2. Analysis of results
As can be seen from the data in Table 5, EC of Bixafen and dimoxystrobin single dose on wheat powdery mildew50EC of bactericide compositions with values of 3.36mg/L and 2.91mg/L, numbers X-1 to X-7 against wheat powdery mildew50The values are respectively 2.33mg/L, 2.18mg/L, 1.93mg/L, 2.01mg/L, 1.99mg/L, 2.24mg/L and 2.33mg/L, and the EC of the bactericide composition of numbers DX-1 to DX-4 on wheat powdery mildew50The value is in the range of 2.73-2.89. As can be seen, the bactericide compositions numbered X-1 to X-7 and DX-1 to DX-4 have EC for wheat powdery mildew50The values are both lower than that of a bixafen single agent and that of an dimoxystrobin single agent. Further, the fungicide compositions of Nos. X-1 to X-7 against wheat powdery mildew EC50The value is obviously smaller than that of a single agent of bixafen and a single agent of dimoxystrobin and is also obviously smaller than that of a comparison sample, namely the bactericide composition numbered DX-1-DX-4.
The result of the co-toxicity coefficient shows that the co-toxicity coefficient of the compound of bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) to the wheat powdery mildew is above 125, and the synergistic effect is achieved; particularly, when the ratio of the bixafen to the dimoxystrobin is 10:1, the co-toxicity coefficient is as high as 171.87, and the synergistic effect is most obvious; the ratio of the two is 20:1 and 1:1, the co-toxicity coefficient is higher than 150, and the co-toxicity coefficient is within the range of 100-120 by compounding according to 40:1, 35:1, 1:35 and 1:40 (samples numbered DX-1-DX-4), and is shown as an additive effect. In conclusion, the toxicity of bixafen and dimoxystrobin is obvious compared with the toxicity of control samples DX-1-DX-4 according to the proportion of (30:1) - (1:30), and the significant synergistic effect of the combination of the bixafen and the dimoxystrobin according to the proportion of (30:1) - (1:30) on the prevention and treatment of the wheat powdery mildew is shown.
5.2 indoor test method for determining toxicity of wheat rust and test result
1. Measurement method
1) And (3) carrying out potted culture on the wheat rust disease plants in the greenhouse.
2) Wheat infected with wheat rust is taken as a test strain, 1 clear water control is set, and 13 medicaments are used for treatment; the 13 medicament treatments respectively correspond to bixafen single dose, dimoxystrobin single dose, bactericide compositions with numbers of X-1-X-7 and bactericide compositions with numbers of DX-1-DX-4, and each treatment is provided with 6 concentration gradients which are 32mg/L, 16mg/L, 8mg/L, 4mg/L, 2mg/L and 1mg/L in sequence; each concentration gradient setup was repeated 4 times.
3) And (3) carrying out spraying treatment by using a Potter spraying tower, wherein the spraying liquid amount is 450L/ha, and clear water is used as a reference for calculating a toxicity equation, EC50 and a co-toxicity coefficient of each medicament.
4) After 15 days of drug application, the prevention and treatment effect is calculated according to the disease index, the DPS data processing software is used for statistical analysis to work out a toxicity regression equation, the EC50 of each drug is calculated, and the cotoxicity coefficient is calculated according to the Sun cloud Peel method.
5) Toxicity analysis is carried out on wheat rust by different bactericide compositions, a bixafen single agent and an dimoxystrobin single agent, and the results are shown in table 6.
TABLE 6 analysis of toxicity of Bixafen and Ether mycoamine in different ratios in suspension bactericide for wheat rust
2. Analysis of results
As can be seen from the data in Table 6, EC of Bixafen and dimoxystrobin single agent on wheat rust50EC of the fungicide compositions with the values of 3.48mg/L and 3.09mg/L respectively and the numbers of X-1 to X-7 on wheat rust50A value in the range of 2.22-2.57; EC of bactericide composition with numbers of DX-1-DX-4 for wheat rust50A value in the range of 2.81-2.96; as can be seen, the bactericide compositions numbered X-1 to X-7 and DX-1 to DX-4 have EC for wheat rust50The values are all below 3.0 and are lower than the EC of bixafen single agent and dimoxystrobin single agent on wheat rust50The value is obtained. Further, the bactericide compositions numbered X-1 to X-7 have EC against wheat rust50The value is obviously smaller than that of a bixafen single agent and an dimoxystrobin single agent, and is also obviously smaller than that of a comparison sample, namely the bactericide composition with the numbers of DX-1-DX-4.
The result of the co-toxicity coefficient shows that the co-toxicity coefficient of the Bixafen and the Etheramine compounded according to the proportion of (30:1) - (1:30) to the wheat rust is more than 120, the synergistic effect is achieved, and particularly when the proportion of the Bixafen to the Etheramine is 10:1, the co-toxicity coefficient is up to 155.21, and the synergistic effect is most obvious; secondly, the ratio of the two is 20:1 and 1:1, and the co-toxicity coefficients are 142.35 and 139.23 respectively; and the co-toxicity coefficient is within the range of 100-120 and is shown as the addition effect according to the combination of 40:1, 35:1, 1:35 and 1:40 (the samples with numbers of DX-1-DX-4). In conclusion, the action trends of bixafen and dimoxystrobin with different ratios on wheat rust and wheat powdery mildew virus are consistent, the toxicity of the bixafen and the dimoxystrobin is obvious compared with the ratio of DX-1-DX-4 according to the ratio of (30:1) - (1:30), and the synergistic effect of the combination of the bixafen and the dimoxystrobin according to the ratio of (30:1) - (1:30) on the prevention and treatment of the wheat rust is obvious.
5.3 indoor test method for determining wheat scab virulence and test result
1. Measurement method
1) And (3) carrying out potted culture on wheat scab diseased plants in a greenhouse.
2) Wheat infected with wheat scab is used as a test strain, 1 clear water control is set, and 13 medicaments are used for treatment; the 13 medicament treatments respectively correspond to bixafen single dose, dimoxystrobin single dose, bactericide compositions with numbers of X-1-X-7 and bactericide compositions with numbers of DX-1-DX-4, and each treatment is provided with 6 concentration gradients which are 32mg/L, 16mg/L, 8mg/L, 4mg/L, 2mg/L and 1mg/L in sequence; each concentration gradient setup was repeated 4 times.
3) And (3) carrying out spraying treatment by using a Potter spraying tower, wherein the spraying liquid amount is 450L/ha, and clear water is used as a reference for calculating a toxicity equation, EC50 and a co-toxicity coefficient of each medicament.
4) After 15 days of drug application, the prevention and treatment effect is calculated according to the disease index, the DPS data processing software is used for statistical analysis to work out a toxicity regression equation, the EC50 of each drug is calculated, and the cotoxicity coefficient is calculated according to the Sun cloud Peel method.
5) Toxicity analysis is carried out on wheat scab through the bactericide composition, a bixafen single agent and an dimoxystrobin single agent, and the experimental results are shown in table 7.
TABLE 7 analysis of toxicity of Bixafen and dimoxystrobin in different ratios in suspension bactericide on wheat scab
2. Analysis of results
As can be seen from the data in Table 7, the EC of Bixafen and dimoxystrobin single dose on wheat scab50Values of 3.71mg/L and 3.40mg/L, respectively, number X-1EEC of X-7 bactericide composition for wheat scab50A value in the range of 2.47-2.77; EC of bactericide composition with numbers of DX-1-DX-4 for wheat scab50A value between 3.08 and 3.20; as can be seen, the bactericide compositions with the numbers of X-1 to X-7 and the numbers of DX-1 to DX-4 have EC for wheat scab50The values of the two agents are lower than the EC of the bixafen single agent and the dimoxystrobin single agent on wheat scab50The value is obtained. Further, the bactericide compositions of numbers X-1 to X-7 have EC for wheat scab50The value is obviously smaller than that of a bixafen single agent and an dimoxystrobin single agent, and is also obviously smaller than that of a comparison sample, namely the bactericide composition with the numbers of DX-1-DX-4.
The result of the co-toxicity coefficient shows that the co-toxicity coefficient of the bixafen and the dimoxystrobin to the wheat scab is more than 120 according to the proportion of (30:1) - (1:30), the synergistic effect is achieved, particularly when the proportion of the bixafen to the dimoxystrobin is 10:1, the co-toxicity coefficient is up to 149.11, and the synergistic effect is most obvious; secondly, the mixture ratio of the two is 20:1, and the co-toxicity coefficient is 139.85; and the co-toxicity coefficient is within the range of 100-120 and shows the addition effect according to the combination of 40:1, 35:1, 1:35 and 1:40 (the samples with the numbers of DX-1-DX-4). In conclusion, the toxicity action trends of bixafen and dimoxystrobin with different ratios to wheat scab are consistent with those of wheat rust and wheat powdery mildew, the toxicity action of the bixafen and the dimoxystrobin is prominent according to the ratios of (30:1) - (1:30) compared with the ratios of DX-1-DX-4, and the compatibility of the bixafen and the dimoxystrobin according to the ratios of (30:1) - (1:30) has a remarkable synergistic effect on the prevention and treatment of wheat scab.
Example 6 field efficacy test
Test time: 2018 years old
Test site: tuping county of Henan province
Test work: wheat (Triticum aestivum L.)
The control object is: rust disease, powdery mildew and gray mold of wheat
The test method comprises the following steps: screening diseased field blocks for test, setting 1 clear water control and treating 13 medicaments; 13 medicament treatments respectively correspond to bixafen single dose, dimoxystrobin single dose and numbers of X-1 to X-7The bactericide composition and the bactericide compositions of DX-1 to DX-4 are respectively provided with 3 spraying dosages of 75ga.i./hm for each treatment2、100ga.i./hm2And 125ga.i./hm2. The fertilizer is uniformly sprayed on the diseased wheat leaves by adopting a leaf surface spraying method. Respectively before and after 3 days, 7 days and 14 days, randomly sampling 5 points in a test treatment area by adopting a 5-point sampling investigation method, grading the disease condition according to relevant standards of national field tests, and calculating the disease condition index and the prevention effect. The test results are shown in tables 8 to 10.
(1) Experimental result of Bixafen and dimoxystrobin with different ratios for preventing and treating wheat rust
The results of experiments on the control of wheat rust by Bixafen single dose, Ethanolamine single dose, the bactericide compositions with the numbers of X-1 to X-7 and the bactericide compositions of DX-1 to DX-4 are shown in Table 8.
TABLE 8 control effect of Bixafen and dimoxystrobin at different ratios on wheat rust
The data in Table 8 show that the measured values are at 75ga.i./hm2、100ga.i./hm2And 125ga.i./hm2Under the three spraying dosages, the control effect of each treatment on the wheat rust disease is increased along with the increase of the using dosage. As can be seen from the data in Table 8, the control effect of the compound combination of bixafen and azoxystrobin on wheat rust is better than that of the single agent of bixafen and the single agent of azoxystrobin, the control effect of 7 agents (the bactericide composition with the number of X-1-X-7) obtained by compounding the bixafen and the azoxystrobin according to the proportion of (30:1) to (1:30) is extremely higher than that of the single agent of bixafen and the single agent of azoxystrobin under the same dosage, and the control effect of the compound combination of bixafen and azoxystrobin is also higher than that of 4 agents (the bactericide composition with the number of DX-1 to DX-4) obtained by compounding according to the ratios of 40:1, 35:1, 1:35 and 1: 40. When the spraying agent is usedTo 150ga.i./hm2When the composition is applied for 7 days, the control effect of the compound agent of bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) on wheat rust is more than 88%, especially when the ratio of bixafen: when the weight ratio of the dimoxystrobin to the dimoxystrobin is 10:1, the control effect of the dimoxystrobin to the wheat rust is as high as 93.36%, and the control effect is remarkably higher than that of a compound medicament and a single medicament in other proportions. The results show that the medicament obtained by compounding the bixafen and the dimoxystrobin according to the proportion of (30:1) - (1:30) has a remarkable synergistic effect on the wheat rust, and the closer the proportion of the bixafen and the azoxystrobin is to 10:1, the better the control effect on the wheat rust is.
(2) Experimental result of Bixafen and dimoxystrobin with different ratios for preventing and treating wheat powdery mildew
The results of experiments on controlling powdery mildew of wheat by using a single agent of bixafen, a single agent of dimoxystrobin, the bactericide compositions with the numbers of X-1 to X-7 and the bactericide compositions of DX-1 to DX-4 are shown in Table 9.
Table 9 shows the control effect of bixafen and dimoxystrobin at different ratios on wheat powdery mildew
The data in Table 9 show that the measured values are at 75ga.i./hm2、100ga.i./hm2And 125ga.i./hm2With the three spraying dosages, the prevention and treatment effect of the wheat powdery mildew of each treatment tends to increase along with the increase of the dosage. As can be seen from the data in Table 9, the control effect of bixafen and azoxystrobin on wheat powdery mildew is better than that of a single bixafen agent and that of a single azoxystrobin agent in combination, the control effect of 7 agents (the bactericide compositions with the numbers of X-1 to X-7) obtained by compounding bixafen and azoxystrobin according to the proportion of (30:1) to (1:30) is remarkably higher than that of the single bixafen agent and that of the single azoxystrobin under the same dosage, and the control effect of the compound is also remarkably higher than that of the single bixafen agent and that of the single azoxystrobin under the same dosage4 medicaments (the bactericide compositions numbered DX-1-DX-4) are obtained by compounding according to the ratio of 40:1, 35:1, 1:35 and 1: 40. When the spraying dosage is 150ga.i./hm2When the composition is applied for 7 days, the control effect of the compound medicament of bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) on wheat powdery mildew is higher than 90%, especially when the weight ratio of the bixafen to the azoxystrobin is 10:1, the control effect on wheat powdery mildew is as high as 94.04%, and the composition is remarkably higher than that of compound medicaments and single agents in other proportions. The results show that the control effect of the agent prepared by compounding bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) on wheat powdery mildew is consistent with that of wheat rust, the agent has a remarkable synergistic effect, and the control effect on wheat powdery mildew is better as the proportion of the bixafen and the azoxystrobin in the agent is closer to 10: 1.
(3) Experimental result of Bixafen and dimoxystrobin with different proportions on prevention and treatment of wheat scab
The results of experiments on the control of wheat scab by Bixafen single dose, dimoxystrobin single dose, the bactericide compositions with the numbers of X-1 to X-7 and the bactericide compositions of DX-1 to DX-4 are shown in Table 10.
Table 10 shows the control effect of bixafen and dimoxystrobin at different ratios on wheat scab
The data in Table 10 show that the measured values are at 75ga.i./hm2、100ga.i./hm2And 125ga.i./hm2Under the three spraying dosages, the prevention and treatment effect on wheat scab is increased along with the increase of the dosage. As can be seen from the data in Table 10, the control effect of the combination of bixafen and azoxystrobin on wheat scab is better than that of the single dose of bixafen and the single dose of azoxystrobin, and the bixafen and the dimoxystrobin are compounded according to the proportion of (30:1) - (1:30)The control effect of 7 medicaments (the bactericide composition with the number of X-1 to X-7) on the wheat scab is remarkably higher than that of a bixafen single agent and an azoxystrobin single agent under the same dosage, and is also remarkably higher than that of 4 medicaments (the bactericide composition with the number of DX-1 to DX-4) compounded according to 40:1, 35:1, 1:35 and 1: 40. When the spraying dosage is 150ga.i./hm2When the composition is applied for 7 days, the control effect of the compound preparation of bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) on wheat scab is higher than 87%, especially when the weight ratio of the bixafen to the azoxystrobin is 10:1, the control effect of the compound preparation on wheat scab is up to 91.57%, and secondly when the weight ratio of the bixafen to the azoxystrobin is 20:1, the control effect of the compound preparation on wheat scab is up to 90.04%, which is remarkably higher than that of the compound preparation and the single preparation in other proportions. The results show that the control effect of the pesticide prepared by compounding bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) on wheat scab is consistent with that of wheat rust and wheat powdery mildew, and the pesticide has obvious synergistic effect, and the control effect on wheat scab is better as the proportion of the bixafen and the azoxystrobin in the pesticide is closer to 10: 1.
By comprehensively analyzing the results in tables 8-10, when the pesticide obtained by compounding bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) has a control effect on wheat scab, wheat rust and wheat powdery mildew which is obviously better than that of a single pesticide of bixafen and azoxystrobin and four pesticides prepared by compounding the single pesticide of bixafen and azoxystrobin according to the proportion of 40:1, 35:1, 1:35 and 1:40, the bactericide composition obtained by compounding bixafen and dimoxystrobin according to the proportion of (30:1) - (1:30) has obvious synergistic effect on wheat scab, wheat rust and wheat powdery mildew, has an excellent control effect on wheat fungal diseases such as wheat rust, wheat powdery mildew and wheat scab, and is worthy of popularization and application in modern agriculture.
Claims (10)
1. The bactericide composition containing bixafen and dimoxystrobin is characterized by comprising the effective components of the bixafen and the dimoxystrobin in a weight ratio of (30:1) - (1: 30).
2. The bactericide composition containing bixafen and dimoxystrobin according to claim 1, wherein the weight ratio of the bixafen to the dimoxystrobin is (20:1) - (1: 10).
3. The bactericide composition containing bixafen and dimoxystrobin according to claim 2, wherein the proportion of the total weight of the bixafen and the dimoxystrobin in the total weight of the bactericide composition is 10-80%.
4. The bactericide composition containing bixafen and dimoxystrobin according to claim 3, wherein the bactericide composition is any one of a suspending agent, an emulsifiable concentrate, a wettable powder and a water dispersible granule.
5. The bactericide composition containing bixafen and dimoxystrobin according to claim 4, further comprising an auxiliary agent; the auxiliary agent is one or more of wetting agent, dispersing agent, filler, emulsifier, thickening agent, disintegrating agent, binder, antifreezing agent and solvent.
6. The germicide composition containing bixafen and dimoxystrobin according to claim 5, which is in the form of suspension, and comprises the following components in parts by weight: the total weight of bixafen and dimoxystrobin is 10-50%, the emulsifier is 3-8%, the thickener is 0.5-2%, the antifreeze is 3-8%, the dispersant is 3-5%, and the water is added to 100%.
7. The bactericide composition containing bixafen and dimoxystrobin according to claim 5, which is in an emulsifiable concentrate form and comprises the following components in parts by weight: the total weight of bixafen and dimoxystrobin is 10-40%, the emulsifier is 9-15%, and the solvent is complemented to 100%.
8. The germicide composition containing bixafen and dimoxystrobin according to claim 5, wherein the germicide composition is water dispersible granules, and the composition by weight is as follows: the total weight of bixafen and dimoxystrobin is 10-80%, the wetting agent is 3-6%, the dispersing agent is 3-5%, the disintegrating agent is 2-6%, the binder is 0.5-3%, and the filler is added to 100%.
9. The germicide composition containing bixafen and dimoxystrobin according to claim 5, wherein the germicide composition is wettable powder, and comprises the following components in parts by weight: the total weight of bixafen and dimoxystrobin is 10-60%, the wetting agent is 5-15%, the dispersant is 3-15%, and the filler is added to 100%.
10. The use of a fungicide according to any one of claims 1 to 9 containing bixafen and dimoxystrobin for the control of powdery mildew, rust disease and head blight of wheat.
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| WO2012019981A1 (en) * | 2010-08-09 | 2012-02-16 | Basf Se | Fungicidal mixtures |
| CN103651425A (en) * | 2012-09-18 | 2014-03-26 | 陕西美邦农药有限公司 | Pesticide composition containing dimoxystrobin |
| CN103907605A (en) * | 2012-12-31 | 2014-07-09 | 江苏丰登农药有限公司 | Composite synergic sterilization composition containing boscalid and bixafen and application thereof |
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| WO2012019981A1 (en) * | 2010-08-09 | 2012-02-16 | Basf Se | Fungicidal mixtures |
| CN103651425A (en) * | 2012-09-18 | 2014-03-26 | 陕西美邦农药有限公司 | Pesticide composition containing dimoxystrobin |
| CN103907605A (en) * | 2012-12-31 | 2014-07-09 | 江苏丰登农药有限公司 | Composite synergic sterilization composition containing boscalid and bixafen and application thereof |
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