CN110346479B - 一种牡蛎内源肽的提取和鉴定方法 - Google Patents
一种牡蛎内源肽的提取和鉴定方法 Download PDFInfo
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- CN110346479B CN110346479B CN201910721301.6A CN201910721301A CN110346479B CN 110346479 B CN110346479 B CN 110346479B CN 201910721301 A CN201910721301 A CN 201910721301A CN 110346479 B CN110346479 B CN 110346479B
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N30/02—Column chromatography
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- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
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Abstract
Description
时间 | A | B |
0 | 95% | 5% |
2 | 95% | 5% |
27 | 80% | 35% |
37 | 65% | 95% |
39 | 20% | 95% |
42 | 20% | 5% |
46 | 95% | 5% |
蛋白鉴定数目 | 肽段鉴定数目 | |
例1 | 0 | 0 |
例2 | 34 | 206 |
例3 | 134 | 696 |
例4 | 605 | 8526 |
时间 | A | B |
0 | 95% | 5% |
2 | 95% | 5% |
27 | 80% | 35% |
37 | 65% | 95% |
39 | 20% | 95% |
42 | 20% | 5% |
46 | 95% | 5% |
时间 | A | B |
0 | 95% | 5% |
2 | 95% | 5% |
27 | 80% | 35% |
37 | 65% | 95% |
39 | 20% | 95% |
42 | 20% | 5% |
46 | 95% | 5% |
时间 | A | B |
0 | 95% | 5% |
2 | 95% | 5% |
27 | 80% | 35% |
37 | 65% | 95% |
39 | 20% | 95% |
42 | 20% | 5% |
46 | 95% | 5% |
时间 | A | B |
0 | 95% | 5% |
0.5 | 95% | 5% |
17 | 65% | 35% |
17.5 | 5% | 95% |
20 | 5% | 95% |
20.1 | 95% | 5% |
25 | 95% | 5% |
时间 | A | B |
0 | 95% | 5% |
0.5 | 95% | 5% |
17 | 65% | 35% |
17.5 | 5% | 95% |
20 | 5% | 95% |
20.1 | 95% | 5% |
25 | 95% | 5% |
Claims (2)
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111398503B (zh) * | 2020-04-16 | 2021-01-15 | 中国农业科学院蜜蜂研究所 | 一种用于检测蜂王浆主蛋白4的试剂盒及检测方法 |
CN113252814A (zh) * | 2021-05-25 | 2021-08-13 | 上海应用技术大学 | 一种大豆蛋白水解物苦味肽的鉴定方法 |
CN114097970A (zh) * | 2021-12-13 | 2022-03-01 | 山东佛坤投资有限公司 | 一种海洋活性肽能量饮料及其制备方法 |
CN114720601B (zh) * | 2022-04-12 | 2023-09-08 | 中国海洋大学 | 三条特征性肽段及其应用 |
Citations (6)
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CN101037468A (zh) * | 2006-03-16 | 2007-09-19 | 中国海洋大学 | 一种牡蛎活性肽的制备方法 |
CN101464430A (zh) * | 2007-12-21 | 2009-06-24 | 中国科学院大连化学物理研究所 | 一种内源性多肽在线富集和自动化分析的方法及专用装置 |
CN103134860A (zh) * | 2011-11-23 | 2013-06-05 | 上海市公共卫生临床中心 | 一种目标多肽和蛋白质定量测定方法 |
CN105866438A (zh) * | 2016-06-08 | 2016-08-17 | 山东出入境检验检疫局检验检疫技术中心 | 一种使用专属性肽段组鉴别美国肉参的方法 |
CN109293740A (zh) * | 2018-10-18 | 2019-02-01 | 大连深蓝肽科技研发有限公司 | 一种牡蛎来源的ace抑制及抗肿瘤活性肽 |
CN109651487A (zh) * | 2019-03-06 | 2019-04-19 | 河南农业大学 | 一种植物体内源肽的提取方法 |
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2019
- 2019-08-06 CN CN201910721301.6A patent/CN110346479B/zh active Active
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CN101037468A (zh) * | 2006-03-16 | 2007-09-19 | 中国海洋大学 | 一种牡蛎活性肽的制备方法 |
CN101464430A (zh) * | 2007-12-21 | 2009-06-24 | 中国科学院大连化学物理研究所 | 一种内源性多肽在线富集和自动化分析的方法及专用装置 |
CN103134860A (zh) * | 2011-11-23 | 2013-06-05 | 上海市公共卫生临床中心 | 一种目标多肽和蛋白质定量测定方法 |
CN105866438A (zh) * | 2016-06-08 | 2016-08-17 | 山东出入境检验检疫局检验检疫技术中心 | 一种使用专属性肽段组鉴别美国肉参的方法 |
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CN109293740A (zh) * | 2018-10-18 | 2019-02-01 | 大连深蓝肽科技研发有限公司 | 一种牡蛎来源的ace抑制及抗肿瘤活性肽 |
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