CN110343680B - 一种金银花查尔酮合酶突变体及其用途 - Google Patents
一种金银花查尔酮合酶突变体及其用途 Download PDFInfo
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- CN110343680B CN110343680B CN201910705623.1A CN201910705623A CN110343680B CN 110343680 B CN110343680 B CN 110343680B CN 201910705623 A CN201910705623 A CN 201910705623A CN 110343680 B CN110343680 B CN 110343680B
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- honeysuckle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12N9/1037—Naringenin-chalcone synthase (2.3.1.74), i.e. chalcone synthase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01074—Naringenin-chalcone synthase (2.3.1.74), i.e. chalcone synthase
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
Description
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 均值 | |
A | 589.6 | 650.9 | 650.2 | 602.3 | 662.4 | 630.6 | 756.9 | 654.5 | 726.5 | 739.4 | 666.3±6.8 |
B | 550.2 | 498.6 | 552.3 | 465.2 | 490.1 | 476.2 | 479.2 | 490.8 | 551.1 | 473.1 | 502.7±8.1 |
C | 389.6 | 435.2 | 450.1 | 360.5 | 320.8 | 420.1 | 413.5 | 460.2 | 386.9 | 401.2 | 403.8±5.6 |
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CN110343680B true CN110343680B (zh) | 2020-03-17 |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002057418A3 (en) * | 2000-12-15 | 2003-08-21 | Salk Inst For Biological Studi | Methods of producing polyketide synthase mutants and compositions and uses thereof |
CN101220353A (zh) * | 2008-01-31 | 2008-07-16 | 北京未名凯拓农业生物技术有限公司 | 一种甘草查尔酮合成酶及其编码基因与应用 |
CN101240266A (zh) * | 2008-03-10 | 2008-08-13 | 西南大学 | 粘毛黄芩查尔酮合成酶蛋白编码序列 |
CN102465133A (zh) * | 2010-11-05 | 2012-05-23 | 中国中医科学院中药研究所 | 金银花查尔酮合酶(LjCHS)基因及其编码的蛋白和应用 |
CN107974484A (zh) * | 2017-11-10 | 2018-05-01 | 嘉兴欣贝莱生物科技有限公司 | 根皮素生物合成过程中查尔酮合成酶建模方法 |
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2019
- 2019-08-01 CN CN201910705623.1A patent/CN110343680B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002057418A3 (en) * | 2000-12-15 | 2003-08-21 | Salk Inst For Biological Studi | Methods of producing polyketide synthase mutants and compositions and uses thereof |
CN101220353A (zh) * | 2008-01-31 | 2008-07-16 | 北京未名凯拓农业生物技术有限公司 | 一种甘草查尔酮合成酶及其编码基因与应用 |
CN101240266A (zh) * | 2008-03-10 | 2008-08-13 | 西南大学 | 粘毛黄芩查尔酮合成酶蛋白编码序列 |
CN102465133A (zh) * | 2010-11-05 | 2012-05-23 | 中国中医科学院中药研究所 | 金银花查尔酮合酶(LjCHS)基因及其编码的蛋白和应用 |
CN107974484A (zh) * | 2017-11-10 | 2018-05-01 | 嘉兴欣贝莱生物科技有限公司 | 根皮素生物合成过程中查尔酮合成酶建模方法 |
Non-Patent Citations (2)
Title |
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0 种观赏植物查尔酮合成酶基因生物信息学分析;张太奎;《西部林业科学》;20131031;第42卷(第5期);图4 * |
金银花chs、fls、chi基因全长克隆及序列分析;乔中全;《中国优秀硕士学位论文全文数据库-农业科技辑》;20121115;第2012年卷;图2-13、2-14、第28页 * |
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Inventor after: Lin Hongjun Inventor after: Gao Peng Inventor after: Jiao Haifeng Inventor after: Tian Wei Inventor after: Dong Juhong Inventor after: Zou Yuyun Inventor after: Guo Yifan Inventor before: Lin Hongjun Inventor before: Gao Peng Inventor before: Jiao Haifeng Inventor before: Tian Wei Inventor before: Dong Juhong Inventor before: Zou Yuyun |
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Denomination of invention: A chalcone synthase mutant of honeysuckle and its application Effective date of registration: 20210421 Granted publication date: 20200317 Pledgee: Jinan finance agricultural technology Financing Guarantee Co.,Ltd. Pledgor: JINAN GUANGSHENGYUAN BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021980002858 |