CN110330104B - Method for removing p-aminobenzoic acid in water body by using microorganisms - Google Patents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
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- C02F2101/38—Organic compounds containing nitrogen
Abstract
The invention discloses a method for removing p-aminobenzoic acid in a water body by using microorganisms, which comprises the steps of enriching and absorbing the p-aminobenzoic acid in the water body by using 3 kinds of fresh water microalgae, then preying on the microalgae by using 2 kinds of wheel worms, preying on the wheel worms by using cladocera named as the Moina spinosa, fishing and filtering the Moina spinosa and residual microalgae and wheel worms by using a plankton net, and discarding filter residues to obtain clean fresh water. The invention has the advantages of simple process, low cost, simple equipment, small occupied area and large-scale production, and the removal rate of the p-aminobenzoic acid reaches 99.5 percent; the cost for treating each ton of fresh water is 0.19 yuan, which is lower than the cost of other treatment methods.
Description
Technical Field
The invention relates to the technical field of environmental pollution treatment, in particular to a method for removing p-aminobenzoic acid in a water body by using microorganisms.
Background
Para aminobenzoic acid (PABA) is a colorless acicular crystal, turns into light yellow in the air or under illumination, is a beige or grey white paste as an industrial product, is used for dye and medical intermediate, and can also be used as a sun-screening agent, but the residue of the PABA is harmful to the environment, is particularly harmful after entering a water body, and has larger difficulty in treating wastewater.
Wuqiang, Chenjiangtang, etc. have studied the ultraviolet/persulfate degradation of aminobenzoic acid in water. The result shows that the ultraviolet/persulfate process can effectively remove PABA, and the removal rate can reach 99%. Under experimental conditions, the removal rate of PABA is increased along with the increase of the addition amount of PDS (persulfate), the pH value of a solution and different ions have little influence on the removal effect of the PABA, HA HAs an inhibiting effect on the degradation of the PABA, and an ultraviolet/persulfate process also HAs a good removal effect on the treatment of a real PABA water sample; however, the method is complicated in technology and high in cost. The Wangdan and the Yuweixiao and the like perform research on the treatment of the anthranilic acid wastewater by an extraction technology, and the COD removal rate of the wastewater reaches 40 percent by adjusting the pH value, so that the biodegradability of the wastewater is improved.
Therefore, the method for removing the p-aminobenzoic acid in the water body, which has the advantages of simple process, low cost, large-scale production and high removal rate of the p-aminobenzoic acid, is a problem to be solved by the technical staff in the field.
Disclosure of Invention
In view of the above, the invention provides a method for removing p-aminobenzoic acid in water by using microorganisms, which has the advantages of simple process, low cost, simple equipment, large-scale production and high removal rate of p-aminobenzoic acid.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for removing p-aminobenzoic acid in a water body by using microorganisms comprises the following specific steps:
(1) adding nutrient salt into water to be treated containing p-aminobenzoic acid, and uniformly stirring;
(2) inoculating fresh water microalgae: sequentially and respectively inoculating the Cross Carde algae, the gloeocystis and the slender crescent moon algae in the water to be treated added with the nutrient salt in the step (1), shaking uniformly, and continuously culturing for 70-74h under the conditions that the temperature is 20-25 ℃, the illumination intensity is 6500-8500lux, and stirring in the period;
the algae used for inoculating the Cross Carde algae, the sacculus algae and the tenuiform crescent moon algae are used for the algae which grows vigorously and is clean and pollution-free in the exponential growth period, and the aged and polluted algae are not used;
(3) transplanting rotifers: when the algae liquid in the step (2) is cultured for 70-74h, inoculating rotifer dissimilis and rotifer forehead sequentially, and keeping for 45-50h under the conditions that the temperature is 20-25 ℃ and the illumination intensity is 6500 and 8500lux, and stirring the mixture;
(4) inoculating Moina magna with thorns: after the step (3), inoculating the Moina spinosa, and keeping for 32-40h under the conditions that the temperature is 20-25 ℃ and the illumination intensity is 6500-;
(5) fishing out plankton by using a plankton net, discarding filter residues and keeping filtrate;
(6) standing in the dark for precipitation, removing sediments, and taking supernate to obtain clean fresh water.
Further, the nutrient salt in the step (1) has the following composition: 2.5mg/L of urea KH2PO40.6mg/L，NaHCO38mg/L, ferric citrate 0.004mg/L and plant growth hormone 0.0002 mg/L.
Various nutrient salts such as urea and KH in the formula when preparing the culture solution2PO4，NaHCO3Ferric citrate, 920 plant growth hormone and the like are added in sequence according to the sequence provided in the formula, and are continuously stirred at the same time so as to avoid chemical reaction; various nutrient salts such as urea, KH are used for preparing culture solution2PO4，NaHCO3The purity of chemical medicines such as ferric citrate, 920 plant growth hormone and the like is required to be in chemical purity level;
further, the inoculation density of the Cross Cardeina in the step (2) is 20 multiplied by 104-30×104The inoculation density of the sacculus is 23 multiplied by 10 per milliliter4-27×104The inoculation density of the crescent moon algae is 40 multiplied by 104-50×104One/ml.
Further, the continuous culture in the step (2) is carried out for 70-74h, and the specific steps during stirring are as follows: the illumination period is 18h of illumination and 6h of darkness; stirring once every 4h, and stirring for 3min each time; when the culture time is 48 hours, adding 1/5 volumes of distilled water to be treated, diluting the algae solution, and continuing to culture for 70-74 hours.
Further, the inoculation density of the Isotailus rotifer in the step (3) is 10-15/ml, and the inoculation density of the Farfugia rhinoceros is 25-35/ml.
Further, the stirring process in the step (3) is to stir once every 6 hours, and each stirring time is 3 min.
Further, the inoculation density of the Moina spinosa in the step (4) is 500 and 700 seeds/liter.
Further, the stirring process in the step (4) is to stir every 3 hours for 3 min.
Further, the step (5) of fishing out plankton by using the plankton net comprises the following steps: filtering with No. 13 plankton net to fish for Daphnia magna, discarding filter residue, and retaining primary filtrate; filtering with 25 # plankton net to obtain residual microalgae and rotifer from the primary filtrate, discarding residue, and retaining secondary filtrate.
The purpose of the step (5) is to use a plankton net to catch or filter plankton in the water body, and the filtering process can be carried out for 2-3 times so as to filter thoroughly; the used plankton nets are sold in the market and can be directly purchased, the No. 13 plankton nets and the No. 25 plankton nets have basically the same structures and are only different in mesh size, and the No. 25 meshes are smaller and are suitable for fishing out smaller plankton; if there is no plankton net, it can be replaced by bolting silk with mesh size of 50-120 μm.
And (3) further, standing and precipitating for 20-28h in the dark in the step (6), removing sediments, and taking supernate to obtain clean fresh water.
According to the technical scheme, compared with the prior art, the invention discloses a method for removing p-aminobenzoic acid in a water body by using microorganisms, 3 kinds of fresh water microalgae are used for enriching and absorbing the p-aminobenzoic acid in the water body, then 2 kinds of wheel insects are used for predating the microalgae, a cladocera named as the daphnia spinosa is used for predating the wheel insects, a plankton net is used for fishing and filtering the daphnia spinosa and residual microalgae and wheel insects, and filter residues are discarded to obtain clean fresh water. The invention has the advantages of simple process, low cost, simple equipment, small occupied area and large-scale production, and the removal rate of the p-aminobenzoic acid reaches 99.5 percent; the cost for treating each ton of fresh water is 0.19 yuan, which is lower than the cost of other treatment methods.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a diagram showing a morphological structure of a Cross cadres used in the present invention;
FIG. 2 is a diagram showing a morphological structure of a chlorella used in the present invention;
FIG. 3 is a drawing showing a structural view of a form of a crescent moon alga used in the present invention;
FIG. 4 is a schematic representation of the xenogeneic rotifer used in the present invention;
FIG. 5 is a schematic representation of a rotifer Melilotus forehead for use in the present invention;
FIG. 6 is a morphogram of Moina magna used in the present invention;
FIG. 7 is a schematic view of the No. 13 plankton net used in the present invention;
FIG. 8 is a schematic view of the No. 25 plankton net used in the present invention;
FIG. 9 is a schematic view of a white plastic bucket used in the present invention, which is the same as the white plastic bucket used in the embodiment.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
920 phytostimulants are purchased from Zhongxin Yihe agricultural Co Ltd, Shouguang, Shandong;
the Oncodera cruciata, the Cystocystis globosa, the crescent moon, the Rotifer sp, the Rotifer forehead and the Moina spinosa are purchased from a microalgae germplasm bank of China university of oceans;
the plankton nets No. 13 and No. 25 were purchased from the purede instruments and equipment ltd, beijing.
Example 1
A method for removing p-aminobenzoic acid in a water body by using microorganisms comprises the following specific steps:
(1) inoculating fresh water microalgae
A 20L white plastic bucket (figure 9) is taken, washed clean,adding 10L of water to be treated containing p-aminobenzoic acid (the content of the p-aminobenzoic acid is 2 percent by weight), and sequentially adding the following nutrient salts: urea 25mg, KH2PO46mg，NaHCO380mg, ferric citrate 0.04mg, and plant growth hormone 920.002 mg, stirring with clean glass rod for 15min to dissolve chemical medicine, sequentially inoculating Cross Carde algae (figure 1), Cystococcus (figure 2), and Tenuioia Tenaci (figure 3), respectively, and culturing 3 kinds of microalgae in the same water body at an inoculation density of 30 × 10 of the cross Carde algae4Perilla frutescens/ml, sacculus algae 25X 10445X 10 pieces/ml of crescent moon algae4And (4) shaking the mixture lightly and uniformly by using a glass rod, and putting the plastic barrel and the culture solution into a microalgae culture chamber for culture. Controlling the temperature of an air conditioner in a culture room, illuminating by an LED energy-saving lamp, controlling the temperature at 23 ℃, controlling the illumination intensity at 7500lux, carrying out open culture, continuously culturing for 72h without aeration, wherein the illumination period is L: d ═ 18 h: stirring with a clean glass rod for 6h (i.e. 18h light, 6h dark) 6 times a day (i.e. every 4 h), each time for 3 min. After 48 hours of culture, 2L of distilled water was added to dilute the algal solution, and the mixture was cultured for 72 hours.
(2) Transplanting rotifer
When the culture of the step (1) is carried out for 72 hours, inoculating 12 Rotifera Heterotrocha (figure 4) and 30 Rotifera forehead (figure 5) into the cultured algae liquid in sequence, so that the Rotifera Heterotrocha and the Rotifera forehead can prey on the microalgae, and simultaneously the Rotifera can also propagate in a large quantity, the illumination and the temperature are the same as those in the step (1), and the prey process is maintained for 48 hours; stirring once every 6h with a clean glass rod, wherein the stirring time lasts 3min each time.
(3) Inoculating Daphnia magna
After the step (2) is finished, transplanting Moina spinosa (figure 6) into the culture solution, and allowing the Moina spinosa to prey on rotifers, wherein the inoculation density of the Moina spinosa is 600 per liter, the illumination and the temperature are the same as those in the step (1), the prey process is maintained for 36 hours, the stirring is carried out once by using a clean glass rod every 3 hours, and the stirring time lasts for 3min each time.
(4) Fishing out plankton by plankton net
After the step (3) is finished, transferring the culture solution into another white plastic barrel (figure 9) with the same size, filtering and fishing the daphnia thorny with a No. 13 plankton net (figure 7) in the transferring process, discarding filter residues, transferring the filtrate into a third white plastic barrel (figure 9), filtering and fishing the residual microalgae and rotifers in the water body with a No. 25 plankton net (figure 8) in the transferring process, discarding the filter residues, and keeping the filtrate.
(5) Standing in dark for precipitation
Transferring the filtrate obtained in the step (4) into a 20L black plastic bucket, standing and precipitating for 24h in the dark, removing sediments, and taking supernate to obtain clean fresh water; the whole processing procedure is ended.
Through measurement, the content of the p-aminobenzoic acid in the treated fresh water is 0.010 percent, and the removal rate is (2-0.010 percent)/2 percent multiplied by 100 percent which is 99.5 percent; namely the removal rate of the p-aminobenzoic acid reaches 99.5 percent; the cost for treating each ton of fresh water is 0.19 yuan.
The cost of waste water treatment is the cost of consumables such as used chemicals, the cost of hydroelectric power and the cost of equipment (the average depreciation of ten years of service life is one year)
Example 2
A method for removing p-aminobenzoic acid in a water body by using microorganisms comprises the following specific steps:
(1) inoculating fresh water microalgae
Taking a 20L white plastic barrel, washing and cleaning, adding 10L water to be treated containing p-aminobenzoic acid (the content of the p-aminobenzoic acid is 2 percent by weight), and sequentially adding the following nutrient salts: urea 25mg, KH2PO46mg，NaHCO380mg, ferric citrate 0.04mg, and plant growth hormone 920.002 mg, stirring with clean glass rod for 15min to dissolve chemical medicine, sequentially inoculating California cruciata, Cystolochia globosa, and Mollungia tenuissima, i.e. 3 kinds of microalgae, respectively, in the same water body, and culturing at an inoculation density of 20 × 104Periplaneta every milliliter, sacculus algae 23X 10450X 10 pieces/ml of crescent moon algae4And (4) shaking the mixture lightly and uniformly by using a glass rod, and putting the plastic barrel and the culture solution into a microalgae culture chamber for culture. The temperature of the air conditioner of the culture room is controlled, the LED energy-saving lamp illuminates, the temperature is controlled at 20 ℃, the illumination intensity is 8500lux, and the room is openedMouth culture, no need of aeration, continuous culture for 70h, and light period L: d ═ 18 h: stirring with a clean glass rod for 6h (i.e. 18h light, 6h dark) 6 times a day (i.e. every 4 h), each time for 3 min. After culturing for 48 hours, 2L of distilled water was added to dilute the algal solution, and the mixture was cultured for 70 hours.
(2) Transplanting rotifer
When the culture in the step (1) is carried out for 70 hours, inoculating 10 Rotifera heterodontia and 35 Rotifera forehead Rotifera in the cultured algae liquid in sequence, so that the Rotifera and the Rotifera prey on microalgae, and simultaneously the Rotifera are propagated in a large amount, the illumination and the temperature are the same as those in the step (1), and the prey process is maintained for 48 hours; stirring once every 6h with a clean glass rod, wherein the stirring time lasts 3min each time.
(3) Inoculating Daphnia magna
After the step (2) is finished, transplanting the Daphnia spinosa into the culture solution, and allowing the Daphnia spinosa to prey on rotifers, wherein the inoculation density of the Daphnia spinosa is 500 per liter, the illumination and the temperature are the same as those in the step (1), the prey process is maintained for 36 hours, the stirring is performed once every 3 hours by using a clean glass rod, and the stirring time lasts for 3min each time.
(4) Fishing out plankton by plankton net
And (3) after the step (3) is finished, transferring the culture solution into another white plastic bucket with the same size, filtering and fishing the daphnia thorny with a No. 13 plankton net in the transferring process, discarding filter residues, transferring the filtrate into a third white plastic bucket, filtering and fishing residual microalgae and rotifers in the water body with a No. 25 plankton net in the transferring process, discarding the filter residues, and keeping the filtrate.
(5) Standing in dark for precipitation
Transferring the filtrate obtained in the step (4) into a 20L black plastic bucket, standing and precipitating for 20h in the dark, removing sediments, and taking supernate to obtain clean fresh water; the whole processing procedure is ended.
Through determination, the content of the p-aminobenzoic acid in the obtained clean fresh water is 0.014%, and the removal rate of the p-aminobenzoic acid reaches 99.3%; the cost for treating each ton of fresh water is about 0.20 yuan.
Example 3
A method for removing p-aminobenzoic acid in a water body by using microorganisms comprises the following specific steps:
(1) inoculating fresh water microalgae
Taking a 20L white plastic barrel, washing and cleaning, adding 10L water to be treated containing p-aminobenzoic acid (the content of the p-aminobenzoic acid is 2 percent by weight), and sequentially adding the following nutrient salts: urea 25mg, KH2PO46mg，NaHCO380mg, ferric citrate 0.04mg, and plant growth hormone 920.002 mg, stirring with clean glass rod for 15min to dissolve chemical medicine, sequentially inoculating California cruciata, Cystolochia globosa, and Mollungia tenuissima, i.e. 3 kinds of microalgae, respectively, in the same water body, and culturing at an inoculation density of California cruciata 25 × 104Perilla frutescens, sacculus algae 27X 104Per ml, crescent moon algae 40X 104And (4) shaking the mixture lightly and uniformly by using a glass rod, and putting the plastic barrel and the culture solution into a microalgae culture chamber for culture. Controlling the temperature of an air conditioner of the culture room, illuminating by an LED energy-saving lamp, controlling the temperature at 25 ℃, controlling the illumination intensity at 6500lux, carrying out open culture, continuously culturing for 74h without aeration, wherein the illumination period is L: d ═ 18 h: stirring with a clean glass rod for 6h (i.e. 18h light, 6h dark) 6 times a day (i.e. every 4 h), each time for 3 min. After 48 hours of culture, 2L of distilled water was added to dilute the algal solution, and the mixture was cultured for 74 hours.
(2) Transplanting rotifer
When the culture of the step (1) is carried out for 74 hours, inoculating the heterodera sp and the forehead rhinoceros sp in the cultured algae liquid in sequence, wherein the inoculation density is 15/ml for the heterodera sp and 25/ml for the forehead rhinoceros sp, so that the rotifers prey on the microalgae, meanwhile, the rotifers also propagate in a large quantity, the illumination and the temperature are the same as those in the step (1), and the prey process is maintained for 48 hours; stirring once every 6h with a clean glass rod, wherein the stirring time lasts 3min each time.
(3) Inoculating Daphnia magna
After the step (2) is finished, transplanting the Daphnia spinosa into the culture solution, and allowing the Daphnia spinosa to prey on rotifers, wherein the inoculation density of the Daphnia spinosa is 700 per liter, the illumination and the temperature are the same as those in the step (1), the prey process is maintained for 36 hours, the stirring is performed once every 3 hours by using a clean glass rod, and the stirring time lasts for 3min each time.
(4) Fishing out plankton by plankton net
And (3) after the step (3) is finished, transferring the culture solution into another white plastic bucket with the same size, filtering and fishing the daphnia thorny with a No. 13 plankton net in the transferring process, discarding filter residues, transferring the filtrate into a third white plastic bucket, filtering and fishing residual microalgae and rotifers in the water body with a No. 25 plankton net in the transferring process, discarding the filter residues, and keeping the filtrate.
(5) Standing in dark for precipitation
Transferring the filtrate obtained in the step (4) into a 20L black plastic bucket, standing and precipitating for 24h in the dark, removing sediments, and taking supernate to obtain clean fresh water; the whole processing procedure is ended.
Through determination, the content of the p-aminobenzoic acid in the obtained clean fresh water is 0.015 percent, and the removal rate of the p-aminobenzoic acid reaches 99.25 percent; the cost for treating each ton of fresh water is about 0.21 yuan.
The method for treating the p-aminobenzoic acid wastewater by using the Suanlan plum of Shenyang chemical research institute design engineering Co., Ltd is superior to the traditional process, and the equipment is not complicated, but the removal rate of the p-aminobenzoic acid is only 85 percent, and the cost is as high as 0.56 yuan/ton of wastewater.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A method for removing p-aminobenzoic acid in a water body by using microorganisms is characterized by comprising the following specific steps:
(1) adding nutrient salt into water to be treated containing p-aminobenzoic acid, and uniformly stirring;
the nutrient salt comprises the following components: 2.5mg/L of urea KH2PO40.6mg/L，NaHCO38mg/L, ferric citrate 0.004mg/L and plant growth hormone 0.0002 mg/L;
(2) inoculating fresh water microalgae: sequentially and respectively inoculating the Cross Carde algae, the gloeocystis and the slender crescent moon algae in the water to be treated added with the nutrient salt in the step (1), shaking uniformly, and continuously culturing for 70-74h under the conditions that the temperature is 20-25 ℃, the illumination intensity is 6500-8500lux, and stirring in the period;
the inoculation density of the Cross Cardeina is 20 × 104-30×104The inoculation density of the sacculus is 23 multiplied by 10 per milliliter4-27×104The inoculation density of the crescent moon algae is 40 multiplied by 104-50×104Per milliliter;
(3) transplanting rotifers: when the algae liquid in the step (2) is cultured for 70-74h, inoculating rotifer dissimilis and rotifer forehead sequentially, and keeping for 45-50h under the conditions that the temperature is 20-25 ℃ and the illumination intensity is 6500 and 8500lux, and stirring the mixture;
the inoculation density of the Isogyrus is 10-15/ml, and the inoculation density of the Farfugia is 25-35/ml;
(4) inoculating Moina magna with thorns: after the step (3), inoculating the Moina spinosa, and keeping for 32-40h under the conditions that the temperature is 20-25 ℃ and the illumination intensity is 6500-;
the inoculation density of the Daphnia magna is 500-700 pieces/liter;
(5) fishing out plankton by using a plankton net, discarding filter residues and keeping filtrate;
(6) standing in the dark for precipitation, removing sediments, and taking supernate to obtain clean fresh water.
2. The method for removing p-aminobenzoic acid from water body with microorganism according to claim 1, wherein the continuous cultivation in step (2) for 70-74h is carried out by the following specific steps during which stirring is carried out: the illumination period is 18h of illumination and 6h of darkness; stirring once every 4h, and stirring for 3min each time; when the culture time is 48 hours, adding 1/5 volumes of distilled water to be treated, diluting the algae solution, and continuing to culture for 70-74 hours.
3. The method for removing p-aminobenzoic acid from water body with microorganism according to claim 1, wherein the stirring process of step (3) is stirring once every 6h for 3 min.
4. The method for removing p-aminobenzoic acid from water body with microorganism according to claim 1, wherein the stirring process of step (4) is stirring once every 3h for 3 min.
5. The method for removing p-aminobenzoic acid from water body with microorganism according to claim 1, wherein the step (5) of scooping plankton with plankton net is as follows: filtering with No. 13 plankton net to fish for Daphnia magna, discarding filter residue, and retaining primary filtrate; filtering with 25 # plankton net to obtain residual microalgae and rotifer from the primary filtrate, discarding residue, and retaining secondary filtrate.
6. The method for removing p-aminobenzoic acid from water body with microorganism according to claim 1, wherein the step (6) is performed by standing and precipitating for 20-28h in dark, discarding the sediment, and taking the supernatant to obtain clean fresh water.
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