CN104430102B - A kind of clam purification Propogation and culture method - Google Patents
A kind of clam purification Propogation and culture method Download PDFInfo
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Abstract
The present invention discloses a kind of clam purification Propogation and culture method, comprise parents holding culture, close shellfish lay eggs, hatch trochophore, D type larva, later stage larva cultivation, juvenile mollusk incubation; Microorganism formulation is thrown in parents holding culture process, parent shellfish process of laying eggs throws in shitosan stain remover, hatching trochophore during throw in microorganism formulation, throw in micro-algae preparation between D type larva, later stage larva culture period, flat algae of throwing something and feeding between juvenile mollusk culture period as bait until finished product results.The present invention all throws in cleaning of substances from parents holding culture, whole seed rearing and young shellfish nurturing period and purifies shellfish meat, the pollution of pollutant is stopped from source, the pollutant such as heavy metal, oil, nitrofurans in the finished product body cultivated significantly reduces, and also has well remove effect to Escherichia coli, less dynamic Sphingol single-cell, vibrios and aeromonas salmonicida etc.
Description
Technical field
The present invention relates to a kind of shellfish Propogation and culture method, be specifically related to a kind of clam purification Propogation and culture method.
Background technology
Clam is under the jurisdiction of gill lamella guiding principle, curtain clam order, Veneridae, is commonly called as black clam, iron clam, clam, circle clam, stone spiral shell etc., is water warm kind, all has distribution China north and south is coastal.Its delicious meat, nutritious, belong to high-protein food.Started since clam carries out artificial breeding research from the eighties in 20th century, clam achieves gratifying achievement in the researchs such as Tu Chi cultivation, factorial seedling growth.
But in recent years, along with the fast development of China's industrial or agricultural, the pollution of pollutant to environment particularly water body is more and more serious, and these pollutants are mainly divided into three major types: one is that industrial pollutants are as heavy metal, oil, agricultural chemicals etc.; Two is microbial contamination, as hepatitis A virus, Escherichia coli etc. derive from the pollution of city domestic sewage; Three is various types of shellfish poisons.Clam locomotivity is weak, and has stronger absorption and accumulation ability for pollutants such as heavy metals, thus in clam the pollutant such as heavy metal very easily enrichment exceed standard.If people have eaten this contaminated clam, intoxicating phenomenon in various degree will be caused.Clam all has strict standard in sale and outlet.Government Of Australia is clear stipulaties once, and the shellfish come into the market must be forced to purify.
At present, the purification method of the shellfishes such as clam mainly contains following several: one is that live body shellfish deviates from technology, transfers in clean water environment and supports temporarily, utilize shellfish own metabolism emission until up to standard by live body shellfish.This method cost is higher, and the clarification time is long and clean-up effect is undesirable; Two is solubility disinfectant purification techniques, and namely utilize chlorine and chlorine-containing compound to purify, chloride has stronger sterilizing ability, and low-level chlorinated compound suppresses shellfish filter food, effectively controls cause of disease.But chloride is chemical reagent, easily produces poisonous chloramines, thus affect shellfish meat; Three is physical methods, and namely adopt ultraviolet or ozone purification, this is also purification method the most frequently used at present.Ultraviolet and ozone purification mode receive the impact of sea water opacity, color, soluble iron salt content, and limitation is larger.
Said method normally by needing the clam finished product of listing to carry out certain purified treatment, to reaching pollutant requirement, also do not have a kind of from the seed rearing stage just purification modes of reproduction, not do not stop the invasion and attack of pollutant from root.
Summary of the invention
Technical problem to be solved by this invention provides a kind of clam to purify Propogation and culture method, purified treatment from close shellfish seed selection, seed breeding, to Propogation and culture to go out in body not containing or the clam finished product of the few pollutant of enrichment, stop the infringement of pollutant from root, ensure the safety of clam.
The present invention is achieved through the following technical solutions:
A kind of clam purification Propogation and culture method:
(1) parents holding culture: annual early June ~ the front 2 ~ 3d of parent shellfish ovulation mid-September, the male and female of results parent shellfish is cleaned up and is put in storage pond, holding pond after removing mud, sand filtration ocean temperature in storage pond, holding pond is adjusted to 20 ~ 22 DEG C, salinity 25 ~ 30, pH7.2 ~ 8.0, keep continuous charge cultivate; Between culture period, constantly in sand filtration seawater, add microorganism formulation; Described microorganism formulation comprises bacillus thuringiensis,Bt freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, gill fungus mushroom entity freeze-dried powder and diatomite;
(2) close shellfish lays eggs: after supporting 24 ~ 48h temporarily, pulling close shellfish out dries in the shade after 12h, put into net cage of hastening parturition be placed in water temperature 26 ~ 28 DEG C, salinity 28 ~ 30, pH7.8 ~ 8.3, water level 1.0 ~ 1.2m, shitosan stain remover 0.01 ~ 0.05g/L water body catalysis pond keep continuous charge to cultivate, full dark processing between culture period; Parent shellfish discharge after fertilization pulls close shellfish out, is 1.5 ~ 1.6m by water level promoting;
(3) trochophore is hatched: pool inner water temperature is adjusted to 25 ~ 28 DEG C, salinity is 20 ~ 25, pH is 7.8 ~ 8.3, light intensity is 300 ~ 500Lx, in pond, throw in microorganism formulation simultaneously, between the trochophore incubation period, continuous charge is cultivated and does not change water body, and constantly input microorganism formulation makes it concentration and remains constant;
(4) D type larva, later stage larva cultivation: after the trochophore in step 3 develops into D type larva, D type larva is pulled out with 300 object bolting silks and is placed in culturing pool, pool inner water temperature is adjusted to 25 ~ 28 DEG C, salinity is 20 ~ 25, pH is 7.8 ~ 8.3, light intensity is 500 ~ 800Lx, after D type larvae development becomes later stage larva, light intensity is adjusted to 800 ~ 1000Lx; Between culture period, continuous charge is cultivated and micro-algae preparation of throwing something and feeding, and every day respectively changes water once sooner or later, and adopt 300 mesh sieve tulle casees to change water, quantity of exchanged water is 1/2 ~ 1/3 of former water body; Described micro-algae preparation comprises the mixture that Skeletonema Greville, flat algae and Dicrateria inornata mix with arbitrary proportion;
(5) juvenile mollusk is cultivated: after the later stage, larva entered juvenile mollusk, be invested in by juvenile mollusk in outdoor nursery pond; Nursery pond is argillo arenaceous, and the dark 1.0m in pond, the depth of water 0.5 ~ 0.8m, water-inflow and drain valve door are separately; The juvenile mollusk nurturing period throws something and feeds flat algae as bait until finished product results.
Preferably, the male and female parent shellfish number ratio described in step 1 is 1:1, and total injected volume of male and female parent shellfish is 1/20 ~ 1/15 of storage pond, holding pond water body weight; Throw in microorganism formulation immediately after clam parent shellfish puts into storage pond, holding pond, upgrade a water body every 4h afterwards, quantity of exchanged water is 1/2 ~ 1/3 of former water body; New microorganism formulation is thrown in immediately after upgrading water body.
Preferably, in microorganism formulation described in step 1 and 3, the weight portion of each component is: bacillus thuringiensis,Bt freeze-dried powder 10 ~ 15 parts, bacillus subtilis freeze-dried powder 5 ~ 8 parts, bacillus licheniformis freeze-dried powder 3 ~ 5 parts, gill fungus mushroom entity freeze-dried powder 3 ~ 7 parts and diatomite 5-10 part; Above-mentioned bacterium powder and diatomite mix and are microorganism formulation.
Preferably, the shitosan cleaner described in step 2 is one or more mixtures mixed with arbitrary proportion in shitosan, modification of chitosan, chitosan derivatives.
Preferably, the microorganism formulation consumption described in step 1 is that 0.01 ~ 0.05g microorganism formulation/L supports water body temporarily; Microorganism formulation concentration described in step 3 maintains 0.05 ~ 0.08g microorganism formulation/L and supports water body temporarily.
Preferably, in step 4, described micro-algae preparation injected volume is: when D type larvae cultivation density is 10 ~ 15/ml, after Measures of Algae in Water Body density 2 ~ 2.5 ten thousand/ml, D type larva grows up into later stage larva, and Measures of Algae in Water Body density 8 ~ 90,000/ml.
Preferably, in step 5, the injected volume of flat algae is 5 ~ 200,000/ml.
The main distinction of the present invention and prior art is: the present invention just purifies the pollutant in close shellfish body from parents holding culture, all throw in cleaning of substances at whole seed rearing and young shellfish nurturing period to purify shellfish meat, the pollution of pollutant is not only stopped from source, and ensure that cultivating process is healthy, green non-pollution, the pollutant such as heavy metal, oil, nitrofurans in the finished product body cultivated significantly reduces, and also has well remove effect to Escherichia coli, less dynamic Sphingol single-cell, vibrios and aeromonas salmonicida etc.; The clam finished product meeting food security completely can be cultivated.
Embodiment
Below in conjunction with specific embodiment, use the present invention is described in detail.
Embodiment 1:
A kind of clam purification Propogation and culture method:
(1) parents holding culture: in Wudi County, Binzhou in annual July ~ mid-September (water temperature 22 ~ 25 DEG C) preovulatory 2 ~ 3d of close shellfish, by the close shellfish of results, (male and female number ratio is 1:1, total injected volume is 1/15 of storage pond, holding pond water body weight) clean up to remove after mud and be put in storage pond, holding pond, sand filtration ocean temperature in storage pond, holding pond is adjusted to 20 ~ 22 DEG C, salinity 20, pH7.5 ~ 7.8, keep continuous charge cultivate; Throw in microorganism formulation immediately after clam parent shellfish puts into storage pond, holding pond, upgrade a water body every 4h afterwards, quantity of exchanged water is 1/2 of former water body, throws in new microorganism formulation immediately after upgrading water body; Described microorganism formulation comprises bacillus thuringiensis,Bt freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, gill fungus mushroom entity freeze-dried powder and diatomite; The weight portion of each component is: bacillus thuringiensis,Bt freeze-dried powder 12 parts, bacillus subtilis freeze-dried powder 6 parts, bacillus licheniformis freeze-dried powder 4 parts, gill fungus mushroom entity freeze-dried powder 5 parts and 7 parts, diatomite; Microorganism formulation consumption is that 0.03g microorganism formulation/L supports water body temporarily;
(2) close shellfish lays eggs: after supporting 48h temporarily, pull out close shellfish dry in the shade to put into after 12h net cage of hastening parturition be placed in water temperature 26 ~ 28 DEG C, salinity 28 ~ 30, pH7.8 ~ 8.3, water level 1.0 ~ 1.2cm, shitosan stain remover 0.02g/L water body catalysis pond keep continuous charge to cultivate, full dark processing between culture period; Parent shellfish discharge after fertilization pulls close shellfish out, is 1.5 ~ 1.6m by water level promoting;
(3) trochophore is hatched: pool inner water temperature is adjusted to 26 ~ 28 DEG C, salinity 22 ~ 25, pH7.8 ~ 8.3, light intensity be 400Lx, in pond, throw in microorganism formulation simultaneously, between the trochophore incubation period, continuous charge is cultivated and does not change water body, and constantly input microorganism formulation makes it concentration and maintains constant at 0.05g microorganism formulation/L foster water body temporarily;
(4) D type larva, later stage larva cultivation: after the trochophore in step 3 develops into D type larva, D type larva is pulled out with 300 object bolting silks and is placed in culturing pool, pool inner water temperature is adjusted to 25 ~ 26 DEG C, salinity is 20 ~ 22, pH is 7.8 ~ 8.3, light intensity is 800Lx, after D type larvae development becomes later stage larva, light intensity is adjusted to 1000Lx; Between culture period, continuous charge is cultivated and micro-algae preparation of throwing something and feeding, and every day respectively changes water once sooner or later, and adopt 300 mesh sieve tulle casees to change water, quantity of exchanged water is 1/2 of former water body; Described micro-algae preparation comprises the mixture that chlorella, flat algae and chlamydomonas mix with arbitrary proportion; Micro-algae preparation injected volume is: when D type larvae cultivation density is 10 ~ 15/ml, after Measures of Algae in Water Body density 2 ~ 2.5 ten thousand/ml, D type larva grows up into later stage larva, and Measures of Algae in Water Body density 8 ~ 90,000/ml;
(5) juvenile mollusk is cultivated: after the later stage, larva entered juvenile mollusk, be invested in by juvenile mollusk in outdoor nursery pond; Nursery pond is argillo arenaceous, and the dark 1.0m in pond, the depth of water 0.5 ~ 0.8m, water-inflow and drain valve door are separately; The juvenile mollusk nurturing period throws something and feeds flat algae (injected volume is 11 ~ 130,000/ml) as bait until finished product results.
Embodiment 2:
(1) parents holding culture: in Wudi County, Binzhou in annual July ~ mid-September (water temperature 22 ~ 25 DEG C) preovulatory 2 ~ 3d of close shellfish, by the close shellfish of results, (male and female number ratio is 1:1, total injected volume is 1/15 of storage pond, holding pond water body weight) clean up to remove after mud and be put in storage pond, holding pond, sand filtration ocean temperature in storage pond, holding pond is adjusted to 20 ~ 22 DEG C, salinity 20, pH7.5 ~ 7.8, keep continuous charge cultivate; Throw in microorganism formulation immediately after clam parent shellfish puts into storage pond, holding pond, upgrade a water body every 4h afterwards, quantity of exchanged water is 1/2 of former water body, throws in new microorganism formulation immediately after upgrading water body; Described microorganism formulation comprises bacillus thuringiensis,Bt freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, gill fungus mushroom entity freeze-dried powder and diatomite; The weight portion of each component is: bacillus thuringiensis,Bt freeze-dried powder 12 parts, bacillus subtilis freeze-dried powder 6 parts, bacillus licheniformis freeze-dried powder 4 parts, gill fungus mushroom entity freeze-dried powder 5 parts and 7 parts, diatomite; Microorganism formulation consumption is that 0.05g microorganism formulation/L supports water body temporarily;
(2) close shellfish lays eggs: after supporting 48h temporarily, pull out close shellfish dry in the shade to put into after 12h net cage of hastening parturition be placed in water temperature 26 ~ 28 DEG C, salinity 28 ~ 30, pH7.8 ~ 8.3, water level 1.0 ~ 1.2cm, shitosan stain remover 0.04g/L water body catalysis pond keep continuous charge to cultivate, full dark processing between culture period; Parent shellfish discharge after fertilization pulls close shellfish out, is 1.5 ~ 1.6m by water level promoting;
(3) trochophore is hatched: pool inner water temperature is adjusted to 26 ~ 28 DEG C, salinity 22 ~ 25, pH7.8 ~ 8.3, light intensity be 400Lx, in pond, throw in microorganism formulation simultaneously, between the trochophore incubation period, continuous charge is cultivated and does not change water body, and constantly input microorganism formulation makes it concentration and maintains constant at 0.06g microorganism formulation/L foster water body temporarily;
(4) D type larva, later stage larva cultivation: after the trochophore in step 3 develops into D type larva, D type larva is pulled out with 300 object bolting silks and is placed in culturing pool, pool inner water temperature is adjusted to 25 ~ 26 DEG C, salinity is 20 ~ 22, pH is 7.8 ~ 8.3, light intensity is 800Lx, after D type larvae development becomes later stage larva, light intensity is adjusted to 1000Lx; Between culture period, continuous charge is cultivated and micro-algae preparation of throwing something and feeding, and every day respectively changes water once sooner or later, and adopt 300 mesh sieve tulle casees to change water, quantity of exchanged water is 1/2 of former water body; Described micro-algae preparation comprises the mixture that chlorella, flat algae and chlamydomonas mix with arbitrary proportion; Micro-algae preparation injected volume is: when D type larvae cultivation density is 10 ~ 15/ml, after Measures of Algae in Water Body density 2 ~ 2.5 ten thousand/ml, D type larva grows up into later stage larva, and Measures of Algae in Water Body density 8 ~ 90,000/ml;
(5) juvenile mollusk is cultivated: after the later stage, larva entered juvenile mollusk, be invested in by juvenile mollusk in outdoor nursery pond; Nursery pond is argillo arenaceous, and the dark 1.0m in pond, the depth of water 0.5 ~ 0.8m, water-inflow and drain valve door are separately; The juvenile mollusk nurturing period throws something and feeds flat algae (injected volume is 11 ~ 130,000/ml) as bait until finished product results.
Embodiment 3:
(1) parents holding culture: in Wudi County, Binzhou in annual July ~ mid-September (water temperature 22 ~ 25 DEG C) preovulatory 2 ~ 3d of close shellfish, by the close shellfish of results, (male and female number ratio is 1:1, total injected volume is 1/15 of storage pond, holding pond water body weight) clean up to remove after mud and be put in storage pond, holding pond, sand filtration ocean temperature in storage pond, holding pond is adjusted to 20 ~ 22 DEG C, salinity 20, pH7.5 ~ 7.8, keep continuous charge cultivate; Throw in microorganism formulation immediately after clam parent shellfish puts into storage pond, holding pond, upgrade a water body every 4h afterwards, quantity of exchanged water is 1/2 of former water body, throws in new microorganism formulation immediately after upgrading water body; Described microorganism formulation comprises bacillus thuringiensis,Bt freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, gill fungus mushroom entity freeze-dried powder and diatomite; The weight portion of each component is: bacillus thuringiensis,Bt freeze-dried powder 12 parts, bacillus subtilis freeze-dried powder 6 parts, bacillus licheniformis freeze-dried powder 4 parts, gill fungus mushroom entity freeze-dried powder 5 parts and 7 parts, diatomite; Microorganism formulation consumption is that 0.04g microorganism formulation/L supports water body temporarily;
(2) close shellfish lays eggs: after supporting 48h temporarily, pull out close shellfish dry in the shade to put into after 12h net cage of hastening parturition be placed in water temperature 26 ~ 28 DEG C, salinity 28 ~ 30, pH7.8 ~ 8.3, water level 1.0 ~ 1.2cm, shitosan stain remover 0.02g/L water body catalysis pond keep continuous charge to cultivate, full dark processing between culture period; Parent shellfish discharge after fertilization pulls close shellfish out, is 1.5 ~ 1.6m by water level promoting;
(3) trochophore is hatched: pool inner water temperature is adjusted to 26 ~ 28 DEG C, salinity 22 ~ 25, pH7.8 ~ 8.3, light intensity be 400Lx, in pond, throw in microorganism formulation simultaneously, between the trochophore incubation period, continuous charge is cultivated and does not change water body, and constantly input microorganism formulation makes it concentration and maintains constant at 0.07g microorganism formulation/L foster water body temporarily;
(4) D type larva, later stage larva cultivation: after the trochophore in step 3 develops into D type larva, D type larva is pulled out with 300 object bolting silks and is placed in culturing pool, pool inner water temperature is adjusted to 25 ~ 26 DEG C, salinity is 20 ~ 22, pH is 7.8 ~ 8.3, light intensity is 800Lx, after D type larvae development becomes later stage larva, light intensity is adjusted to 1000Lx; Between culture period, continuous charge is cultivated and micro-algae preparation of throwing something and feeding, and every day respectively changes water once sooner or later, and adopt 300 mesh sieve tulle casees to change water, quantity of exchanged water is 1/2 of former water body; Described micro-algae preparation comprises the mixture that chlorella, flat algae and chlamydomonas mix with arbitrary proportion; Micro-algae preparation injected volume is: when D type larvae cultivation density is 10 ~ 15/ml, after Measures of Algae in Water Body density 2 ~ 2.5 ten thousand/ml, D type larva grows up into later stage larva, and Measures of Algae in Water Body density 8 ~ 90,000/ml;
(5) juvenile mollusk is cultivated: after the later stage, larva entered juvenile mollusk, be invested in by juvenile mollusk in outdoor nursery pond; Nursery pond is argillo arenaceous, and the dark 1.0m in pond, the depth of water 0.5 ~ 0.8m, water-inflow and drain valve door are separately; The juvenile mollusk nurturing period throws something and feeds flat algae (injected volume is 11 ~ 130,000/ml) as bait until finished product results.
Comparative trial:
The comparative trial simultaneously purified, that is: compare with traditional clam seedling raising manners:
Random choose 20 clam examination volume internal contamination situations are often organized before experiment, the processes such as parent shellfish is conventionally supported with the inventive method subsequently respectively temporarily, dry in the shade, after cultivating close shellfish finished product, often organize random choose 20 clam finished product inspection purifications: i.e. " marine monitoring specification: offshore pollution Ecological Investigation and biological monitoring " E. coli counts adopts GB17378.7-1998 counting; Vibrios water sample on foot on TCBS medium, by standard Plating counts after cultivating 24h at 35 DEG C after dilution; Heavy metal Pb, Cd, Cu adopt graphite furnace atomic absorption spectrometry, and Zn adopts flame atomic absorption spectrometry, and Hg adopts atomic fluorescence spectrum to measure:
Result is as shown in table 1:
Table 1: conventional method and clam finished product internal pollution thing statistical conditions of the present invention
As shown in Table 1, the finished product that the inventive method Propogation and culture goes out, the content of beary metal in its body, bacterial content, well below national standard, meet the food safety requirements of shellfish meat completely, can skip purifying step and directly go on the market.
Above-mentioned example just for technical conceive of the present invention and technical characterstic are described, can not limit the scope of the invention with this.The equivalent transformation that all essence according to the present invention is done or modification, all should be encompassed within protection scope of the present invention.
Claims (7)
1. a clam purification Propogation and culture method, is characterized in that, comprise the following steps:
(1) parents holding culture: annual early June ~ the front 2 ~ 3d of parent shellfish ovulation mid-September, be put in storage pond, holding pond after the clam of results parent shellfish is cleaned up, sand filtration ocean temperature in storage pond, holding pond is adjusted to 20 ~ 22 DEG C, salinity 20 ~ 25, pH7.2 ~ 8.0, keep continuous charge cultivate; Between culture period, constantly in sand filtration seawater, add microorganism formulation; Described microorganism formulation comprises bacillus thuringiensis,Bt freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, gill fungus mushroom entity freeze-dried powder and diatomite;
(2) close shellfish lays eggs: after supporting 24 ~ 48h temporarily, pull out close shellfish dry in the shade to put into after 12h net cage of hastening parturition be placed in water temperature 26 ~ 28 DEG C, salinity 22-25, pH7.8 ~ 8.3, water level 1.0 ~ 1.2m, shitosan stain remover 0.01 ~ 0.05g/L water body catalysis pond keep continuous charge to cultivate, full dark processing between culture period; Parent shellfish discharge after fertilization pulls close shellfish out, is 1.5-1.6m by water level promoting;
(3) hatch trochophore: pool inner water temperature is adjusted to 25 ~ 28 DEG C, salinity 20 ~ 25, pH7.8 ~ 8.3, light intensity 300 ~ 500Lx, in pond, throw in microorganism formulation simultaneously, between the trochophore incubation period, continuous charge is cultivated and does not change water body, and constantly input microorganism formulation makes it concentration and remains constant;
(4) D type larva, later stage larva cultivation: after the trochophore in step 3 develops into D type larva, D type larva is pulled out with 300 mesh sieve thin,tough silk and is placed in culturing pool, water temperature 25 ~ 28 DEG C, salinity 20 ~ 25, pH7.8 ~ 8.3, light intensity is kept to be 600 ~ 800Lx pond environment, after D type larvae development becomes later stage larva, light intensity is adjusted to 800 ~ 1000Lx; Between culture period, continuous charge is cultivated and micro-algae preparation of throwing something and feeding, and every day respectively changes water once sooner or later, and adopt 300 mesh sieve tulle casees to change water, quantity of exchanged water is 1/2 ~ 1/3 of former water body; Described micro-algae preparation comprises the mixture that Skeletonema Greville, flat algae and Dicrateria inornata mix with arbitrary proportion;
(5) juvenile mollusk is cultivated: after the later stage, larva entered juvenile mollusk, be invested in by juvenile mollusk in outdoor nursery pond; Nursery pond is argillo arenaceous, and the dark 1.0m in pond, the depth of water 0.5 ~ 0.8m, water-inflow and drain valve door are separately; The juvenile mollusk nurturing period throws something and feeds flat algae as bait until finished product results.
2. method according to claim 1, is characterized in that, the male and female parent shellfish number ratio described in step 1 is 1:1, and the total injected volume of male and female parent Bei is 1/20 ~ 1/15 of storage pond, holding pond water body weight; Throw in microorganism formulation immediately after clam parent shellfish puts into storage pond, holding pond, upgrade a water body every 4h afterwards, quantity of exchanged water is 1/2 ~ 1/3 of former water body; New microorganism formulation is thrown in immediately after upgrading water body.
3. method according to claim 1, it is characterized in that, in microorganism formulation described in step 1 and 3, the weight portion of each component is: bacillus thuringiensis,Bt freeze-dried powder 10 ~ 15 parts, bacillus subtilis freeze-dried powder 5 ~ 8 parts, bacillus licheniformis freeze-dried powder 3 ~ 5 parts, gill fungus mushroom entity freeze-dried powder 3 ~ 7 parts and 5 ~ 10 parts, diatomite; Above-mentioned bacterium powder and diatomite mix and are microorganism formulation.
4. method according to claim 1, is characterized in that, the shitosan cleaner described in step 2 is one or more mixtures mixed with arbitrary proportion in shitosan, modification of chitosan, chitosan derivatives.
5. method according to claim 1, is characterized in that, the microorganism formulation consumption described in step 1 is that 0.01 ~ 0.05g microorganism formulation/L supports water body temporarily; Microorganism formulation concentration described in step 3 maintains 0.05 ~ 0.08g microorganism formulation/L and supports water body temporarily.
6. method according to claim 1, is characterized in that, in step 4, described micro-algae preparation injected volume is: when D type larvae cultivation density is 10 ~ 15/ml, after Measures of Algae in Water Body density 2 ~ 2.5 ten thousand/ml, D type larva grows up into later stage larva, Measures of Algae in Water Body density 8 ~ 90,000/ml.
7. method according to claim 1, is characterized in that, in step 5, the injected volume of flat algae is 5 ~ 200,000/ml.
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| CN112568157B (en) * | 2020-12-04 | 2022-11-25 | 浙江省海洋水产养殖研究所 | Breeding method of tegillarca granosa strain with low cadmium enrichment |
| CN112704032B (en) * | 2020-12-29 | 2021-12-21 | 中国水产科学研究院黄海水产研究所 | Method and device for reducing norovirus in seawater shellfish |
| CN114190313B (en) * | 2021-11-25 | 2023-04-11 | 舒城县汇德水产科技有限公司 | Environment-friendly high-density red swamp crayfish breeding and seedling method |
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| JP4393254B2 (en) * | 2004-04-07 | 2010-01-06 | ヤンマー株式会社 | Bivalve purification method, bivalve purification determination method, and bivalve purification device |
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