CN102583767A - System for treating sewage and producing biological oil by using microalgae and method - Google Patents
System for treating sewage and producing biological oil by using microalgae and method Download PDFInfo
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- CN102583767A CN102583767A CN201110008373XA CN201110008373A CN102583767A CN 102583767 A CN102583767 A CN 102583767A CN 201110008373X A CN201110008373X A CN 201110008373XA CN 201110008373 A CN201110008373 A CN 201110008373A CN 102583767 A CN102583767 A CN 102583767A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a system for treating sewage and producing biological oil by using microalgae. A first light source device and a second light source device of the system are respectively arranged in a photo-biological reactor and an oil accumulating tank; a CO2 gas supply device is respectively connected with the photo-biological reactor and the oil accumulating tank through air passages; a sewage sedimentation tank is connected with the oil accumulating tank through a pipeline and connected with the photo-biological reactor via a pipeline through a microalgal liquid mixing tank; the photo-biological reactor is respectively connected with the microalgal liquid mixing tank and the oil accumulating tank via pipelines through a first microalgal liquid separating device; and the oil accumulating tank is connected with a second separating device through a pipeline. The invention further provides a method for using the system to treat sewage and produce biological oil. Through the use of the invention, the production of biological energy sources and other byproducts (such as protein, cosmetic raw materials, animal feed and the like) is realized while sewage is treated, so that the production cost is lowered, and the comprehensive utilization of waste resources and the energy-saving and emission-reducing green production are achieved. The invention is suitable for cultivating microalgae from a laboratory to a factory, is skillful and unique in design, and is applicable to large-scale promotion and application.
Description
Technical field
The present invention relates to environmental engineering and bioenergy technical field, more specifically, relate to the bioreactor technical field, be meant especially and a kind ofly utilize little algae to dispose of sewage and produce the system and method for bio-oil.
Background technology
Now, energy scarcity, environmental pollution and short-commons are three big world difficult problems.Little algae has important effect in solving an above-mentioned difficult problem.
Little algae is a kind ofly can carry out photoautotrophic mikrobe, has light utilization efficiency efficiently, and from environment, draws nutritive substance quickly and efficiently (like CO
2, nitrogen, phosphorus etc.) and change into the ability of organic cpds, in the ecosystem, be in the primary producer, in whole material cycle effect huge, production efficiency is high, development prospect is tempting.Little algae is cultivated only needs some simple nutrition, ammonium salt or nitrate salt, and phosphoric acid salt and some micro-metalss also need the most important thing is a large amount of CO
2And little algae also may be used in the environmental engineering, carries out WWT and helps purify waste water.Little algae can reduce the concentration of the material that causes eutrophication in the sewage, like nitrate salt, phosphoric acid salt etc.In addition, little algae also has very strong receptivity to heavy metal, thereby the enrichment that can also carry out heavy metal or radioactive substance reaches the removing effect.And little algae is rich in grease, protein, glucide, trace element and other biologically active substance, thereby it not only can be used as bait, and is one of main source of food and bioenergy.
Current, environmental pollution is serious, and a large amount of sewage can not get effective processing, and factory produces a large amount of CO
2Greenhouse gases can not get handling.Algae culture is for WWT, CO
2Reduce discharging and the clean biometric Energy production with utilize the vital role of aspect and the important meaning of improving the ecological environment, make the research and development of little algae become one of the most popular at present research and Application Areas.Though little algae has higher environment and economic worth, really to realize the utilization of little algae industrialization economy, still be subject to the development of the bioreactor of little algae industrially scalable cultivation.
In order to solve the problems referred to above of existence, a kind of system that utilizes little algae to dispose of sewage and produce bio-oil need be provided, in WWT; Realization production bioenergy and other sub products (as; Protein, cosmetic material and animal-feed etc.), reduce production costs, can reach the comprehensive utilization of waste resource; Realize the green production of energy-saving and emission-reduction, be suitable for little algae cultivation from the laboratory to the industrial scale.
Summary of the invention
Main purpose of the present invention is exactly the problems and shortcomings to above existence, provides a kind of and utilizes little algae to dispose of sewage and produce the system and method for bio-oil, in WWT; Realization production bioenergy and other sub products (as; Protein, cosmetic material and animal-feed etc.), reduce production costs, can reach the comprehensive utilization of waste resource; Realize the green production of energy-saving and emission-reduction; Be suitable for little algae cultivation from the laboratory to the industrial scale, design ingenious uniqueness, be suitable for large-scale promotion application.
In first aspect of the present invention, a kind of system that utilizes little algae to dispose of sewage and produce bio-oil is provided, be characterized in, comprise bioreactor, first light supply apparatus, CO
2Air feeder, long-pending oil tank, secondary light source device, detention tank, algae liquid mixing tank, the first algae liquid separating apparatus and the second algae liquid separating apparatus; Said first light supply apparatus is arranged in the said bioreactor; Said secondary light source device is arranged in the said long-pending oil tank, said CO
2Air feeder gas circuit respectively connects said bioreactor and said long-pending oil tank; Said detention tank pipeline connects said long-pending oil tank and connects said bioreactor through said algae liquid mixing tank pipeline; Said bioreactor through the said first algae liquid separating apparatus respectively pipeline connect said algae liquid mixing tank and said long-pending oil tank, said long-pending oil tank pipeline connects the said second algae liquid separating apparatus.
For the CO that disperses better to feed
2Gas, preferably, the described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises at least one CO
2Gas distributor, said CO
2Gas distributor is arranged in the said bioreactor and the outer said CO with being positioned at said bioreactor
2The air feeder pipeline connects.
Said CO
2Gas distributor can adopt any suitable shape, more preferably, and said CO
2Gas distributor is annular CO
2Gas distributor.
Said CO
2Gas distributor can adopt any suitable size, more preferably, and said CO
2The diameter of gas distributor is 0.2～0.6 with the length of said bioreactor or wide ratio, with the bottom of said bioreactor at a distance of 10～50cm, and said CO
2Pore diameter on the gas distributor is 0.2～2.0mm.
For culturing microalgae better, more preferably, the described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises whipping appts, said whipping appts comprise drive element and with said CO
2The same number of stirring rake of gas distributor, said stirring rake is arranged on said CO
2The top of gas distributor, said drive element are positioned at the outer and part of said bioreactor and wear said bioreactor and connect said stirring rake.
The volume of said bioreactor can be any, and preferably, the volume of said bioreactor is 100m
3～10000m
3
The shape of said bioreactor can be any suitable shape, and preferably, said bioreactor is a sealing slot type bioreactor, and is the full of twists and turns cell body that at least two U-shapeds are connected to form.
Said sealing slot type bioreactor can be transparent; Also can be opaque; More preferably; Said sealing slot type bioreactor is transparent sealing slot type bioreactor, can adopt other transparent materials such as glass or polymkeric substance, and said first light supply apparatus is sunshine light source and/or LED three look cold light sources.For example said sunshine light source can adopt the Fresnel condensing apparatus to carry out the collection of sunshine, and said LED three look cold light sources can be that waterproof, anticorrosion and explosion-proof LED are red, orange and blue three look cold light light sources, and power is 100～400W.
Said long-pending oil tank can be transparent, also can be opaque, and more preferably, said long-pending oil tank is transparent long-pending oil tank, said secondary light source device is sunshine light source and/or LED three look cold light sources.
In order to control culture temperature better; Preferably; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises temperature-control device; Said temperature-control device comprises multistage temperature control chuck or coil pipe, and said multistage temperature control chuck or coil pipe are arranged on the outer wall of said sealing slot type bioreactor.
Preferably, the heating mode that utilizes little algae to dispose of sewage and produce the system of bio-oil of the present invention heats up for waste heat or the well heater that utilizes heat power plant to produce.
In order to sponge the oxygen that culturing microalgae produces, preferably, the described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises oxygen separator, and said oxygen separator is built in the vertical collection and confinement of gases pipe of said bioreactor.Said oxygen separator is preferably the device that the strong reductant that absorbs oxygen is housed.
In order in time to understand the cultivation situation; Preferably; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises at least one photosensitive probe, TM, pH electrode and dissolved oxygen electrode, and wall and its probe position that said photosensitive probe, said TM, said pH electrode and said dissolved oxygen electrode wear said bioreactor respectively are positioned at said bioreactor.
Said CO
2Air feeder can be any suitable device, preferably, and said CO
2Air feeder comprises industrial CO
2Waste gas storage tank, air and gas mixing tank, said industrial CO
2Waste gas storage tank and said air gas circuit respectively are connected said gas mixing tank, and said gas mixing tank gas circuit respectively connects said bioreactor and said long-pending oil tank.
For the ease of discharging isolating sewage, preferably, the said first algae liquid separating apparatus is provided with first water outlet, and the said second algae liquid separating apparatus is provided with second water outlet.
In order to remove through the sewage in the nutrient solution that bioreactor is cultivated; Preferably; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises settling pond, and said bioreactor connects the said first algae liquid separating apparatus through said settling pond pipeline.
For the ease of discharging isolating sewage, more preferably, said settling pond is provided with the 3rd water outlet.
For the ease of collecting the isolating frond of the second algae liquid separating apparatus, preferably, the described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises holding tank, and the said second algae liquid separating apparatus pipeline connects said holding tank.
In second aspect of the present invention, provide a kind of the employing above-mentioned utilize little algae to dispose of sewage and produce the system handles sewage of bio-oil and the method for producing bio-oil, be characterized in the little algae of inoculation in said bioreactor, said CO
2Air feeder provides 15.0%～30.0% (v/v) CO
2Gas, said detention tank hold municipal wastewater and/or the secondary effluent of industrial sewage after sewage work handles, with said secondary effluent and said CO
2Gas feeds and cultivates said little algae in the said bioreactor; The water outlet of said bioreactor obtains concentrating algae liquid through the said first algae liquid separating apparatus; The said concentrated algae liquid of a part gets into said algae liquid mixing tank and mixes the said bioreactor of entering afterwards with said secondary effluent from said detention tank, with said secondary effluent and said CO
2Gas feeds said long-pending oil tank, and the said concentrated algae liquid of another part gets into said long-pending oil tank and cultivates, and the water outlet of said long-pending oil tank obtains being used to extract the frond of algae oil through the said second algae liquid separating apparatus.
Said CO
2Gas can be the CO in any source
2Gas.Preferably, said CO
2Gas is that factory's exhaust gas discharged and air mixed are formed.Can directly mix, also can to waste gas do certain processing (as, dedusting, sulphur removal etc.) the back remix, thereby effectively utilize waste gas, and reduce to pollute.
Said secondary effluent is the second pond water outlet of sewage work's second-stage treatment technology, and nitrogen phosphorus equal size generally is more or less the same in the secondary effluent of different sewage treatment plant.Usually, total nitrogen is 30.0～50.0mg/L in the said secondary effluent, and ammonia nitrogen is 10.0～16.0mg/L, and total phosphorus concentration is 2.0～4.0mg/L, and pH is 6.5～8.5.
Said little algae can be adopted any suitable little algae, and general requirement is to contain grease height, CO
2Tolerance reaches the high advantage algae kind of removal efficiency of nitrogen and phosphorus by force; Preferably, said little algae is selected from one or more of autotrophy chlorella (Chlorella autotuophica), little plan ball algae (Nannochloropsis salina), Blang's grape algae (Botyococcus Braunii), very thin Chaetoceros (ChaetocerosGracilis), extra large Chlorococcum (Halochlorococcum marinum), Isochrysis galbana (Isochrysis galbana Parke), Nitzschia closterium minutissima (Nitzschia closterium), Phaeodactylum tricornutum (Phaeodactylum tricornutum Bohlin), Dunaliella salina (Dunaliella sp.) and prismatic algae (Nitzschia sp.).
Said Chlorella vulgaris can adopt any suitable Chlorella vulgaris, and more preferably, said Chlorella vulgaris is chlorella C9-JN2010, and deposit number is: CCTCC NO:M 2010373, initial inoculation density is 1.0 * 10
7Cfu/ml～6.0 * 10
7Cfu/ml, said culture condition is: periodicity of illumination is 10h: 14h～16h: 8h, and intensity of illumination is 5000lux～15000lux, and culture temperature is 20～30 ℃, and pH is 6.5～8.5, CO
2The gas ventilation amount is 0.01～0.2VVm.
Said Chlorella vulgaris is from the seawater in Huanghai Sea marine site, to separate and pass through artificially breeding to obtain; Called after chlorella C9-JN2010; For green, single spherical frond, wall approach, formal name used at school was categorized as a kind of Chlorella vulgaris (Chlorella vulgaris), and it is deposited in Chinese typical culture collection center (China Center for Type CultureCollection on December 31st, 2010; Be called for short CCTCC), the culture presevation registration number is CCTCC NO:M 2010373.
Cut apart cultivation through high-density, the batch processing time can shorten to 12～24h.
Beneficial effect of the present invention is:
A. bioreactor of the present invention, first light supply apparatus, CO
2Air feeder, long-pending oil tank, secondary light source device, detention tank, algae liquid mixing tank, the first algae liquid separating apparatus and the second algae liquid separating apparatus; Said first light supply apparatus is arranged in the said bioreactor; Said secondary light source device is arranged in the said long-pending oil tank, said CO
2Air feeder gas circuit respectively connects said bioreactor and said long-pending oil tank, and said detention tank pipeline connects said long-pending oil tank and connects said bioreactor through said algae liquid mixing tank pipeline, said bioreactor through the said first algae liquid separating apparatus respectively pipeline connect said algae liquid mixing tank and said long-pending oil tank; Said long-pending oil tank pipeline connects the said second algae liquid separating apparatus, thereby disposes of sewage and culturing microalgae through bioreactor, mixes back back light bio-reactor with B-grade sewage after wherein a part of little algae concentrates and cultivates; Another part gets into long-pending oil tank and cultivates long-pending oil; In WWT, realization production bioenergy and other sub products (as, protein, cosmetic material and animal-feed etc.); Reduce production costs; Can reach the comprehensive utilization of waste resource, realize the green production of energy-saving and emission-reduction, be suitable for little algae cultivation from the laboratory to the industrial scale; Design ingenious uniqueness, be suitable for large-scale promotion application.
B. bioreactor of the present invention is transparent sealing slot type bioreactor, and said first light supply apparatus is sunshine light source and/or LED three look cold light sources, utilizes CO
2Gas distributor distribution CO
2Gas, and stirring rake is set up, the waste heat or the well heater that around sealing slot type bioreactor, are provided with temperature-control device and adopt heat power plant to produce heat up; Reactor internal components is less, and the utilising efficiency of light is high, bubble is evenly distributed, and gas-liquid mixed and mass transfer effect are good; Whole culture systems shearing force is very little, is beneficial to the growth of little algae, and is rational in infrastructure; Easy to operate, have good frustule growing environment, light, CO
2Utilising efficiency is high, and power consumption is little, and reactor drum takes up room little, and equipment cost is low, is applicable to that (volume of slot type bioreactor is 100m to industrially scalable in the laboratory
3～10000m
3) little algae cultivation, be suitable for large-scale promotion application.
C. the method for disposing of sewage and producing bio-oil of the present invention is a substratum with municipal wastewater and industrial sewage secondary effluent; Need not add nutritive elements such as nitrogen phosphorus; Can alleviate the water resources burden and reduce WWT and bioenergy production cost, carry out WWT and CO
2Can obtain bioenergy when reducing discharging, design is unique ingenious, is a kind of green production, reutilization of the sewage and bioenergy production technology cheaply, is suitable for large-scale promotion application.
D. adopt the method for disposing of sewage and producing bio-oil of the present invention, can realize nitrogen, phosphorus and CO
2High removal efficient (as, total nitrogen concentration is lower than 15.0mg/L in the water, and ammonia nitrogen concentration is less than 1.0mg/L, and total phosphorus concentration is lower than 0.5mg/L, and the nitrogen and phosphorus content in the sewage has reduced by 80.0%～98.0% (W) and 80.0%～99.5% (W), CO respectively
2Concentration reduces by 30.0%～75.0% (v/v)), and concentration of algae and content of oil and grease are high, and (concentration of algae that is respectively in the photoreactor reaches 1.0 * 10
8Cfu/ml～5.0 * 10
8Cfu/ml, its fat content are 25%～40% (W)), the batch processing time can shorten to 12～24h, can reach the double effects that protection environment and Biological resources are produced again, realizes high economic and social synthesis's benefit, is suitable for large-scale promotion application.
Description of drawings
Fig. 1 is that each parts of a specific embodiment that utilize little algae to dispose of sewage and produce the system of bio-oil of the present invention connect synoptic diagram.
Fig. 2 is the schematic perspective view of the transparent sealing slot type bioreactor of specific embodiment shown in Figure 1.
Fig. 3 is that the master of transparent sealing slot type bioreactor shown in Figure 2 looks synoptic diagram.
Fig. 4 is the schematic top plan view of transparent sealing slot type bioreactor shown in Figure 2.
Fig. 5 is the schematic side view of transparent sealing slot type bioreactor shown in Figure 2.
Fig. 6 A adopts the method for disposing of sewage and producing bio-oil of the present invention to cut apart to cultivate nitrogen, phosphorus and the CO that chlorella C9-JN2010 disposes of sewage
2The removal effect synoptic diagram.
Fig. 6 B adopts the method for disposing of sewage and producing bio-oil of the present invention to cut apart to cultivate fatty accumulation effect synoptic diagram in living weight that chlorella C9-JN2010 disposes of sewage and the born of the same parents.
Fig. 7 A adopts of the present inventionly to dispose of sewage and produce nitrogen, phosphorus and the CO that the method cultured continuously chlorella C9-JN2010 of bio-oil disposes of sewage
2The removal effect synoptic diagram.
Fig. 7 B adopts of the present inventionly to dispose of sewage and produce fatty accumulation effect synoptic diagram in living weight that the method cultured continuously chlorella C9-JN2010 of bio-oil disposes of sewage and the born of the same parents.
Fig. 8 A adopts of the present inventionly to dispose of sewage and produce nitrogen, phosphorus and the CO that the method batch culture chlorella C9-JN2010 of bio-oil disposes of sewage
2The removal effect synoptic diagram.
Fig. 8 B adopts of the present inventionly to dispose of sewage and produce fatty accumulation effect synoptic diagram in living weight that the method batch culture chlorella C9-JN2010 of bio-oil disposes of sewage and the born of the same parents.
Fig. 9 A is nitrogen, phosphorus and the CO that adopts slot type bioreactor of the present invention and common air lift type photoreactor batch culture chlorella C9-JN2010 to dispose of sewage respectively
2The removal effect synoptic diagram.
Fig. 9 B is the living weight effect synoptic diagram that adopts slot type bioreactor of the present invention and common air lift type photoreactor cultured continuously chlorella C9-JN2010 to dispose of sewage respectively.
Fig. 9 C adopts fatty accumulation effect synoptic diagram in the born of the same parents that slot type bioreactor of the present invention and common air lift type photoreactor batch culture chlorella C9-JN2010 dispose of sewage respectively.
The parts that Reference numeral among the wherein above-mentioned figure is represented are:
1. bioreactor 2. amasss oil tank 3. industrial CO
2The waste gas storage tank
4. gas mixing tank 5. first light supply apparatuses 6. settling ponds 7. algae liquid mixing tanks
8. detention tank 9. air 10. first algae liquid separating apparatus 11. algae liquid holding tanks
12. pump 13. valves 14. gas meter 15.CO
2Gas distributor
16. stirring rake 17. oxygen separator 18.CO
2Inlet mouth 19. opening for feeds/inoculation mouth
20. discharge gate 21. circulating water intakes 22. circulating water outlets 23. temperature control chuck/coil pipes
24. photosensitive probe 25. TM 26.pH electrodes 27. dissolved oxygen electrodes
28. collection and confinement of gases pipe 29. manholes 30. venting port 31.CO
2Detector
32. pressure warning unit 33. second algae liquid separating apparatus
Embodiment
In order more to be expressly understood technology contents of the present invention, the special following examples of lifting specify.Wherein identical parts adopt identical Reference numeral.
See also shown in Fig. 1-5, the system that utilizes little algae to dispose of sewage and produce bio-oil of the present invention comprises bioreactor 1, and it is transparent sealing slot type bioreactor; Making full of twists and turns wavyly, see also Fig. 2 and shown in Figure 4, specifically is the full of twists and turns cell body that two U-shapeds are connected to form; Thereby form 4 inner chambers, be arranged in parallel, the substratum in it will flow along inner chamber is full of twists and turns; Can in the space of compactness, quicken heat passage and the increase light-receiving area, industrial CO
2Waste gas storage tank 3 and air 9 gas circuit respectively are connected said gas mixing tank 4; Said gas mixing tank 4 gas circuit respectively connects said bioreactor 1 and said long-pending oil tank 2; Be installed in first light supply apparatus 5 and secondary light source device (not shown) in the long-pending oil tank 2 in the bioreactor 1 and be solar source or/and the waterproof of 100W, anticorrosion and explosion-proof type LED three look cold light sources; The LED three-color light source is connected with the system (not shown) through lead; Bioreactor 1 connects the said first algae liquid separating apparatus 10 through settling pond 6 pipelines; Said detention tank 8 pipelines connect said long-pending oil tank 2 and connect said bioreactor 1 through said algae liquid mixing tank 7 pipelines; The said first algae liquid separating apparatus 10 pipeline respectively connects said algae liquid mixing tank 7 and said long-pending oil tank 2, and said long-pending oil tank 2 connects holding tank 11 through the said second algae liquid separating apparatus, 33 pipelines.
Said CO
2Gas distributor 15 be arranged in the said bioreactor 1 and be positioned at said bioreactor 1 outer said gas mixing tank 4 pipelines and be connected.Said CO
2Gas distributor 15 is annular CO
2Gas distributor.Said CO
2The diameter of gas distributor 15 is 0.2～0.6 with the length or the wide ratio of each inner chamber of said bioreactor 1, with the bottom of said bioreactor 1 at a distance of 10～50cm, and said CO
2Pore diameter on the gas distributor 15 is 0.2～2.0mm.Said stirring rake 16 is arranged on said CO
2The top of gas distributor 15, said drive element (not shown) are positioned at said bioreactor 1 outer also part and wear the said stirring rake 16 of said bioreactor 1 connection, and said drive element is connected with system through lead.The volume of said bioreactor 1 is 100m
3, its working volume is 90m
3The outer wall of said bioreactor is provided with multistage temperature control chuck or coil pipe 23; Be respectively equipped with circulating water intake 21 and circulating water outlet 22 at the two ends of chuck/coil pipe 23; The waste heat or the well heater that can utilize heat power plant to produce heat up, and on the wall of the left and right sides of bioreactor 1, are respectively arranged with opening for feed/inoculation mouth 19 and discharge gate 20, opening for feed/inoculation mouthful 19 connection detention tanks 8; Sewage substratum and algae kind can pump into bioreactor 1 through opening for feed/inoculation mouth 19; Be provided with the detection of taking a sample of thief hole (not shown) at discharge gate 20, cultivate the algae liquid of accomplishing and discharge, flow to settling pond 6 then through discharge gate 20; Around the wall of bioreactor 1, be provided with photosensitive probe 24, TM 25, pH electrode 26 and dissolved oxygen electrode 27, and be connected with system through lead; On bioreactor 1, be provided with collection and confinement of gases pipe 28; Be provided with oxygen separator 17 in the collection and confinement of gases pipe 28 and carry out the collection of oxygen; Be provided with manhole 29 in the side of collection and confinement of gases pipe 28 in addition; And be provided with venting port 30 on the top of collection and confinement of gases pipe 28, and unnecessary gas is discharged, on venting port 30, be respectively equipped with pressure warning unit 32 and CO
2Detector 31, CO
2Detector 31 is connected with system through lead; Front at bioreactor 1 is provided with CO
2Thereby inlet mouth 18 and the wall that wears bioreactor 1 through gas tube with its in CO
2Gas distributor 15 is connected, CO
2Inlet mouth 18 is connected with gas mixing tank 4.
And be respectively equipped with under meter 14 and valve 13 on all gas pipings, be respectively equipped with liquid pump 12 on the liquid transmission pipeline and carry out flow control with valve 13.
The little algae of inoculation in bioreactor 1 is with the CO of industrial discharge
2Waste gas is compressed in industrial CO
2In the waste gas storage tank 3, and with mix in gas mixing tank 4 by the good air of air 9 compression, adopt valve 13 and under meter 14 to come the flow size of pilot-gas to make CO
2Concentration is 15%～30% (v/v); Feed bioreactor 1 and long-pending oil tank 2 then respectively; Bioreactor 1 cultured algae liquid pump is gone into settling pond 6 clarification back obtain the tertiary treatment water and discharge from the 3rd water outlet, the algae dredge pump that is deposited in settling pond 6 lower curtates is gone into the first algae liquid separating apparatus 10, and the crown_interception through the first algae liquid separating apparatus 10 obtains tertiary treatment sewage and gets into first water outlet and discharge; Divide two portions and concentrate the dense algae liquid that obtains; A part pumps into algae liquid mixing tank 7, pumps into algae liquid mixing tank 7 to the secondary effluent of detention tank 8 simultaneously and mixes, and subsequently the algae liquid pump of back-mixing is gone into bioreactor 1; And can cut apart or cultured continuously with the algae cell density of control bioreactor 1 through the whole reflux ratio of controlling flow velocity modulation; The dense algae liquid pump of another part is gone into to amass and is cultivated long-pending oil in the oil tank 2, will amass the algae liquid pump of oil subsequently and go into the second algae liquid separating apparatus 33, and the crown_interception through the second algae liquid separating apparatus 33 obtains tertiary treatment sewage and gets into second water outlet and discharge again; Concentrate the dense algae liquid that obtains and be pumped into holding tank 11, carry out the refining of algae oil after the collection.
(deposit number is following mask body: CCTCC NO:M 2010373) be example with Chlorella vulgaris-chlorella C9-JN2010; Adopt and of the present inventionly utilize little algae to dispose of sewage and produce the system handles sewage of bio-oil and produce bio-oil, initial inoculation density is 1.0 * 10
7Cfu/ml～6.0 * 10
7Cfu/ml, culture condition is: periodicity of illumination is 10h: 14h～16h: 8h, and intensity of illumination is 5000lux～15000lux, and culture temperature is 20～35 ℃, and pH is 6.5～8.5, CO
2The gas ventilation amount is 0.01～0.2VVm.
The transformation efficiency of nitrogen, phosphorus is that clearance is an important indicator weighing this process spent water treatment effect in the waste water, and method of calculation are as follows:
The transformation efficiency of nitrogen, phosphorus (%)=(C
1-C
2) * 100%/C
1
In the formula: C
1The content of initial nitrogen, phosphorus in the-wastewater medium, mg;
C
2The content of nitrogen, phosphorus in the-processed waste water nutrient solution, mg.
CO in the waste gas
2Transformation efficiency be CO
2CER is an important indicator weighing this technology waste gas treatment effect, and method of calculation are as follows:
CO
2Transformation efficiency (%)=(C
1-C
2) * 100%/C
1
In the formula: C
1CO in the-power plant waste gas
2Volumetric concentration, %;
C
2-CO in the waste gas after handling
2Volumetric concentration, %.
The content of microalgae grease or greasy yield are to weigh an important indicator of the overall economic efficiency of this technology, and method of calculation are as follows:
Greasy yield (%)=m
1/ m
0* 100%
In the formula: m
0The greasy frond dry weight of-extraction, g;
m
1-thick greasy quality, g.
Shake bottle and plant the general f/2 substratum (pH7.0) of liquid cultivation employing green alga, seed tank culture and bioreactor 1 are cultivated and are all adopted sewage substratum (pH7.0).The sewage substratum is that (total nitrogen is 30.0～50.0mg/L to the secondary effluent of handling through sewage work with municipal wastewater behind filtering and impurity removing bacterium or the assorted algae and industrial sewage; Ammonia nitrogen is 10.0～16.0mg/L; Total phosphorus concentration is the substratum of 2.0～4.0mg/L) formation; Join in the bioreactor 1 and sterilize through opening for feed/inoculation mouthfuls 19, initial inoculation density is 6.0 * 10
7Cfu/ml, mixing speed are 120rpm, and intensity of illumination is 15000lux, and periodicity of illumination is 16h: 8h, and culture temperature is 30 ℃, and pH is 7.0, CO
2The CO of gas
2Concentration is 30% (v/v), and air flow is 0.1VVm, cut apart cultivation before, cultivate behind the 48h in the said bioreactor 1 concentration of algae up to 2.0 * 10
8Cfu/ml; And the nitrogen in the said biological photoreactor 1, the content of phosphorus meet national category-A discharge water standard, and (total nitrogen concentration is 15.0mg/L; Ammonia nitrogen concentration is 1.0mg/L; Total phosphorus concentration is 0.5mg/L) time carries out fermented liquid and cuts apart, and takes out 1/3～2/3 said fermented liquid and concentrates and obtain concentrated broth, the said concentrated broth of a part returned in the said bioreactor 1 also the secondary effluent of adding detention tank 8 in the said bioreactor 1; The total amount 100% that keeps said substratum, the concentration of algae that continues to be cultured in the said bioreactor 1 reaches 5.0 * 10
8Cfu/ml.Remaining said concentrated broth being pumped in the have the sewage substratum long-pending oil tank 2 of (wherein adding 2.5% sea salt (W)) at air flow is the CO of 0.2VVm
2Concentration is the said CO of 30.0% (v/v)
2Two stage fermentation carries out oil and fat accumulation 48h under the gas, collects frond afterwards and extracts algae oil.
Utilize above-mentioned technology to cultivate chlorella C9-JN2010, nitrogen, phosphorus and CO
2Clearance and oil and fat accumulation relation see shown in Fig. 6 A-6B.
Analyzed and can be known by Fig. 6 A, in 0～48h culturing process, along with incubation time prolongs, chlorella C9-JN2010 is to nitrogen, phosphorus and CO
2Spending rate accelerate, the fastest at the 48h spending rate, reaches peak and be respectively 90.0% (W), 93.0% (W) and 70% (v/v), and frond concentration reaches 2.0 * 10
8Cfu/ml is cut apart subsequently, takes out after wherein 2/3 fermented liquid concentrates, with wherein 1/3 Returning reactor and charge into salt-free fresh municipal wastewater, keeps substratum total amount 100%, and a part feeds long-pending oil tank 2 Secondary Fermentations and amasss oily 48h in addition.Analyzed and can be known by Fig. 6 B, final concentration of algae reaches 5.0 * 10
8Cfu/ml, fat content are up to 40.0% (W).Cut apart cultivation once more by above-mentioned later on, semicontinuous cultivation is cut apart 10 times continuously.
Continuously ferment mode and be that the secondary effluent of handling through sewage work is a substratum with municipal wastewater behind filtering and impurity removing bacterium or the assorted algae and industrial sewage of employing, to this inoculation of medium chlorella C9-JN2010, wherein, initially connecing density is 3.5 * 10
7Cfu/ml, periodicity of illumination are 14h: 10h, and intensity of illumination is 5000lux, and culture temperature is 25 ℃, and pH is 8.5, CO
2The CO of gas
2Concentration is 25.0% (v/v), and air flow is 0.2VVm, cultured continuously 480h, nitrogen, phosphorus removal effect, CO
2Transformation efficiency (% (v/v)) and oil and fat accumulation relation see shown in Fig. 7 A-7B.
Learn that by Fig. 7 A analysis in cultured continuously in earlier stage, along with the time lengthening of cultivating, it is to nitrogen, phosphorus and CO
2Utilization accelerate.The speed of utilizing at 0～120h nitrogen is constantly accelerated, and the subsequent removal rate reaches 80.0% (W), and also unstable at 120～288h, nitrogen removal efficiency tends towards stability at 80.0% (W) subsequently.The speed of utilizing at 0～96h phosphorus is constantly accelerated, and the subsequent removal rate reaches 80.0% (W), and 96～216h is also unstable, and tp removal rate tends towards stability at 80.0% (W) subsequently.At 0～96h CO
2Utilize speed constantly to accelerate, the subsequent removal rate reaches 65.0% (v/v), and is also unstable at 96～240h, subsequently CO
2Clearance tends towards stability at 65.0% (v/v).
Learn that by Fig. 7 B analysis in cultured continuously in earlier stage, along with the time lengthening of cultivating, frustule growth velocity and greasy accumulation rate are accelerated.Constantly accelerate in 0～96h frond speed of growth, frond concentration reaches 3.0 * 10
8Cfu/ml, also unstable at 96～240h, frond concentration tends towards stability 3.0 * 10 subsequently
8Cfu/ml.0～192h oil and fat accumulation speed constantly accelerate and fat content up to 25.0% (W), the frond content of oil and grease tends towards stability at 25.0% (W) after the 192h.
Adopt the batch fermentation mode and be to be substratum through secondary effluent from sewage treatment plant with municipal wastewater behind filtering and impurity removing bacterium or the assorted algae and industrial sewage, to this inoculation of medium chlorella C9-JN2010, wherein, initially connecing density is 1.0 * 10
7Cfu/ml, periodicity of illumination are 10h: 14h, and intensity of illumination is 7500lux, and culture temperature is 20 ℃, and pH is 6.5, CO
2Concentration be 15.0% (v/v), air flow is 0.01VVm, batch culture 480h, nitrogen, phosphorus removal effect, CO
2Transformation efficiency (% (v/v)) and oil and fat accumulation relation see shown in Fig. 8 A-8B.
Learn that by Fig. 8 A analysis cultivating early stage, along with the time lengthening of cultivating, it is to nitrogen, phosphorus and CO
2Utilization accelerate.In 0～168h nitrogen utilize speed constantly accelerate and clearance up to 98.0% (W), in 168～216h, remain on this level subsequently always, but begin to drop to 91.0% (W) at 240h, remain on this level afterwards always.In 0～168h phosphorus utilize speed constantly accelerate and clearance up to 99.5% (W), in 168～264h, remain on this level subsequently always, but slightly descend at 288h, be 98.0% (W), remain on this level afterwards always.CO in 0～168h
2Utilize continuous quickening of speed and clearance up to 75.0% (v/v), and in 168～240h, keep stable, utilize speed to slow down gradually subsequently, final CO
2Clearance has only 30.0% (v/v).
Learn that by Fig. 8 B analysis in batch culture in earlier stage, along with the time lengthening of cultivating, frustule growth velocity and greasy accumulation rate are accelerated.In the batch culture process, constantly accelerate and frond concentration up 1.1 * 10 in the growth of 0～120h frond
8Cfu/ml maintains this level in 120h～168h, frond concentration begins to descend subsequently, finally is 5.2 * 10
7Cfu/ml.Continuous quickening of frond oil and fat accumulation speed and frond fat content remain on this level subsequently up to 30.0% (W) always in 0～168h.
According to aforesaid method; (deposit number is: batch culture CCTCC NO:M 2010373) to adopt transparent slot type bioreactor of the present invention and airlift photobioreactor to carry out Chlorella vulgaris C9-JN2010 respectively; Culture cycle is 240h, nitrogen, phosphorus removal effect, CO
2Transformation efficiency (%) and oil and fat accumulation relation see shown in Fig. 9 A-9C.
Learn by Fig. 9 A analysis, adopt in the slot type bioreactor batch culture process nitrogen, phosphorus and CO
2Utilize rapid speed and remove the rate best result and do not reach 98.0%, 99.5% and 75.0%, and adopt in the common airlift photobioreactor batch culture process nitrogen, phosphorus and CO
2Utilize speed slower, its clearance best result is not 82.0%, 85.0% and 65.0%.
Learnt that by Fig. 9 B analysis adopt in the slot type bioreactor batch culture process, the frond growth is also very fast, in 0～96h, along with the prolongation of incubation time, the frond speed of growth is very fast, and the living weight of final algae is the highest by 1.2 * 10
8Cfu/ml, the frond living weight begins to descend subsequently, and adopts in the common airlift photobioreactor batch culture process; The frond growth is also very fast, in 0～144h, along with the prolongation of incubation time; The frond speed of growth is very fast, and the living weight of final algae is the highest by 1.0 * 10
8Cfu/ml, the frond living weight begins to descend subsequently.
Learn by Fig. 9 C analysis, adopt in the slot type bioreactor batch culture process, in 0～144h; Along with the prolongation of incubation time, oil and fat accumulation rapid speed in the frond cell, the fat content of final algae is up to 30.0%; Fat content begins to descend in the cell subsequently; And adopt in the common airlift photobioreactor batch culture process, in 0～144h, oil and fat accumulation rapid speed in the frond cell; The fat content of final algae is up to 25.0%, and fat content begins to descend in the cell subsequently.
Therefore, of the present inventionly be substratum and utilize Chlorella vulgaris C9-JN2010 high-density culture technology to carry out WWT that with municipal wastewater and industrial sewage to this inoculation of medium chlorella C9-JN2010, wherein, initially connecing density is 1.0 * 10
7Cfu/ml～6.0 * 10
7Cfu/ml, periodicity of illumination are 10h: 14h～16h: 8h, and intensity of illumination is 5000lux～15000lux, and culture temperature is 20～30 ℃, and pH is 6.5～8.5, CO
2(15.0%～30.0% (v/v)) air flow is 0.01～0.2VVm; After cultivating 480h; Frustule concentration is high; And nitrogen, phosphorus equal size in the said biological photoreactor 1 meet national category-A discharge water standard (total nitrogen concentration is 15.0mg/L, and ammonia nitrogen concentration is 1.0mg/L, and total phosphorus concentration is 0.5mg/L).After the advanced treatment through chlorella, total nitrogen concentration is lower than 15.0mg/L in the water, and ammonia nitrogen concentration is less than 1.0mg/L, and total phosphorus concentration is lower than 0.5mg/L, and the nitrogen and phosphorus content in the sewage has reduced by 80.0%～98.0% (W) and 80.0%～99.5% (W), CO respectively
2Concentration reduces by 30.0%～75.0% (v/v), and pH is 7.0～8.0.Through the growth accumulation grease of chlorella, the concentration of algae in the finally long-pending oil tank 2 reaches 1.0 * 10
8Cfu/ml～5.0 * 10
8Cfu/ml, fat content can reach 25.0%～40.0% (W).Can realize CO
2Reduce discharging and bioenergy production, nitrogen, phosphorus and CO
2High remove efficient, and concentration of algae, content of oil and grease are high, reach the double effects of protection environment and production Biological resources, easy and simple to handle fast, low, pollution-free, the society of cost and economical synthesis benefit height, be suitable for large-scale promotion application.
And adopt slot type bioreactor and common airlift photobioreactor to cultivate Chlorella vulgaris C9-JN2010 respectively; The slot type bioreactor is compared with common airlift photobioreactor; The slot type bioreactor more helps greasy accumulation in growth and the cell of frustule, and the fat content best result is not 1.2 * 10 in living weight and the frustule
8Cfu/ml and 30.0% (W) has all improved about 20.0% than the latter, simultaneously to nitrogen phosphorus and CO in the sewage
2Removal effect also better, maximum material removal rate is respectively 98.0% (W), 99.5% (W) and 75.0% (v/v), its clearance has improved 19.5%, 17.0% and 15.4% respectively than the latter.
When microalgae for carrying out sewage advanced treatment of the present invention and when producing greasy device main body for sealing slot type bioreactor; Utilizing it to carry out little algae cultivates; Because sealing slot type bioreactor can clean and sterilization completely; Though culture condition is gentle, still can keep cultivating the pollution-free state of whole process; In little algae culturing process, do not need vigorous stirring, the nutrient solution liquid motion has stronger regularity in the sealing slot type bioreactor; Shearing action is little; When cultivating chlorella cells, microscopically is observed the frond cell and is sphere, smooth surface, smooth even; Because sealing slot type bioreactor outer wall is provided with a plurality of temperature control chucks or coil pipe 23, can carry out sectional temperature-controlled and stable temperature control.Because the light-receiving area/volume ratio of sealing slot type bioreactor is bigger, uses high concentration CO
2The time, energy consumption is little and poison smallly, be difficult for to produce conditions such as oxygen accumulation and murder by poisoning.The present invention is fit to carry out the cultivation of various microalgae cells, and all can reach higher cell density.Do not have powerful mechanical stirring in the reactor drum, need not feed a large amount of lower concentration CO yet
2And consume a large amount of energy consumptions.Simultaneously, the present invention is in operation, and each link such as inoculation, sampling, collection frond, cleaning, sterilization and dismounting are all very convenient quick.The present invention can use separately, also can a plurality ofly be combined into the serial or parallel connection culture systems together, can realize little algae large-scale industrialization high-density culture.
In sum, the system and method that utilizes little algae to dispose of sewage and produce bio-oil of the present invention in WWT, realize to produce bioenergy and other sub products (as; Protein, cosmetic material and animal-feed etc.), reduce production costs, can reach the comprehensive utilization of waste resource; Realize the green production of energy-saving and emission-reduction; Be suitable for little algae cultivation from the laboratory to the industrial scale, design ingenious uniqueness, be suitable for large-scale promotion application.
In this specification sheets, the present invention is described with reference to its certain embodiments.But, still can make various modifications and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, specification sheets and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (21)
1. a system that utilizes little algae to dispose of sewage and produce bio-oil is characterized in that, comprises bioreactor, first light supply apparatus, CO
2Air feeder, long-pending oil tank, secondary light source device, detention tank, algae liquid mixing tank, the first algae liquid separating apparatus and the second algae liquid separating apparatus; Said first light supply apparatus is arranged in the said bioreactor; Said secondary light source device is arranged in the said long-pending oil tank, said CO
2Air feeder gas circuit respectively connects said bioreactor and said long-pending oil tank; Said detention tank pipeline connects said long-pending oil tank and connects said bioreactor through said algae liquid mixing tank pipeline; Said bioreactor through the said first algae liquid separating apparatus respectively pipeline connect said algae liquid mixing tank and said long-pending oil tank, said long-pending oil tank pipeline connects the said second algae liquid separating apparatus.
2. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1 is characterized in that, the described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises at least one CO
2Gas distributor, said CO
2Gas distributor is arranged in the said bioreactor and the outer said CO with being positioned at said bioreactor
2The air feeder pipeline connects.
3. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 2 is characterized in that, said CO
2Gas distributor is annular CO
2Gas distributor.
4. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 3 is characterized in that, said CO
2The diameter of gas distributor is 0.2～0.6 with the length of said bioreactor or wide ratio, with the bottom of said bioreactor at a distance of 10～50cm, and said CO
2Pore diameter on the gas distributor is 0.2～2.0mm.
5. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 2; It is characterized in that; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises whipping appts, said whipping appts comprise drive element and with said CO
2The same number of stirring rake of gas distributor, said stirring rake is arranged on said CO
2The top of gas distributor, said drive element are positioned at the outer and part of said bioreactor and wear said bioreactor and connect said stirring rake.
6. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1 is characterized in that, the volume of said bioreactor is 100m
3～10000m
3
7. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1 is characterized in that, said bioreactor is a sealing slot type bioreactor, and is the full of twists and turns cell body that at least two U-shapeds are connected to form.
8. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 7; It is characterized in that; Said sealing slot type bioreactor is transparent sealing slot type bioreactor, and said first light supply apparatus is sunshine light source and/or LED three look cold light sources.
9. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 7 is characterized in that, said long-pending oil tank is transparent long-pending oil tank, and said secondary light source device is sunshine light source and/or LED three look cold light sources.
10. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1; It is characterized in that; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises temperature-control device; Said temperature-control device comprises multistage temperature control chuck or coil pipe, and said multistage temperature control chuck or coil pipe are arranged on the outer wall of said sealing slot type bioreactor.
11. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1; It is characterized in that; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises oxygen separator, and said oxygen separator is built in the vertical collection and confinement of gases pipe of said bioreactor.
12. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1; It is characterized in that; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises at least one photosensitive probe, TM, pH electrode and dissolved oxygen electrode, and wall and its probe position that said photosensitive probe, said TM, said pH electrode and said dissolved oxygen electrode wear said bioreactor respectively are positioned at said bioreactor.
13. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1 is characterized in that, said CO
2Air feeder comprises industrial CO
2Waste gas storage tank, air and gas mixing tank, said industrial CO
2Waste gas storage tank and said air gas circuit respectively are connected said gas mixing tank, and said gas mixing tank gas circuit respectively connects said bioreactor and said long-pending oil tank.
14. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1 is characterized in that, the said first algae liquid separating apparatus is provided with first water outlet, and the said second algae liquid separating apparatus is provided with second water outlet.
15. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1; It is characterized in that; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises settling pond, and said bioreactor connects the said first algae liquid separating apparatus through said settling pond pipeline.
16. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 15 is characterized in that said settling pond is provided with the 3rd water outlet.
17. the system that utilizes little algae to dispose of sewage and produce bio-oil according to claim 1; It is characterized in that; The described system that utilizes little algae to dispose of sewage and produce bio-oil also comprises holding tank, and the said second algae liquid separating apparatus pipeline connects said holding tank.
18. one kind is adopted and according to claim 1ly to utilize little algae to dispose of sewage and produce the system handles sewage of bio-oil and the method for producing bio-oil, it is characterized in that the little algae of inoculation in said bioreactor, said CO
2Air feeder provides 15.0～30.0% (v/v) CO
2Gas, said detention tank hold municipal wastewater and/or the secondary effluent of industrial sewage after sewage work handles, with said secondary effluent and said CO
2Gas feeds and cultivates said little algae in the said bioreactor; The water outlet of said bioreactor obtains concentrating algae liquid through the said first algae liquid separating apparatus; The said concentrated algae liquid of a part gets into said algae liquid mixing tank and mixes the said bioreactor of entering afterwards with said secondary effluent from said detention tank, with said secondary effluent and said CO
2Gas feeds said long-pending oil tank, and the said concentrated algae liquid of another part gets into said long-pending oil tank and cultivates, and the water outlet of said long-pending oil tank obtains being used to extract the frond of algae oil through the said second algae liquid separating apparatus.
19. method according to claim 18 is characterized in that, total nitrogen is 30.0～50.0mg/L in the said secondary effluent, and ammonia nitrogen is 10.0～16.0mg/L, and total phosphorus concentration is 2.0～4.0mg/L.
20. method according to claim 18; It is characterized in that said little algae is selected from one or more of autotrophy chlorella, little plan ball algae, Blang's grape algae, very thin Chaetoceros, extra large Chlorococcum, Isochrysis galbana, Nitzschia closterium minutissima, Phaeodactylum tricornutum, Dunaliella salina and prismatic algae.
21. method according to claim 20 is characterized in that, said Chlorella vulgaris is chlorella C9-JN2010, and deposit number is: CCTCC NO:M 2010373, initial inoculation density is 1.0 * 10
7Cfu/ml～6.0 * 10
7Cfu/ml, said culture condition is: periodicity of illumination is 10h: 14h～16h: 8h, and intensity of illumination is 5000lux～15000lux, and culture temperature is 20～30 ℃, and pH is 6.5～8.5, CO
2The gas ventilation amount is 0.01～0.2VVm.
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| CN102815839A (en) * | 2012-08-27 | 2012-12-12 | 哈尔滨工业大学 | Method and device for low-carbon emission energy regeneration by coupling waste gas and waste water |
| CN104524964A (en) * | 2014-12-18 | 2015-04-22 | 中国科学院广州能源研究所 | Device and method for cleaning carbon dioxide in industrial waste gas via microalgae |
| WO2015085631A1 (en) * | 2013-12-13 | 2015-06-18 | 嘉兴泽元生物制品有限责任公司 | Method for culturing botryococcus spp. with high yield |
| CN106883982A (en) * | 2017-03-27 | 2017-06-23 | 嘉兴学院 | Pot type bioreactor and its helotisn body synchronous purification biogas biogas slurry method |
| CN107098478A (en) * | 2017-06-23 | 2017-08-29 | 四川省天惠能源科技有限公司 | A kind of microalgae carbon sequestration and sewage ecological treatment system |
| CN110218748A (en) * | 2019-06-19 | 2019-09-10 | 北京林业大学 | A method of grease is produced using microalgae low cost |
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|---|---|---|---|---|
| CN102815839A (en) * | 2012-08-27 | 2012-12-12 | 哈尔滨工业大学 | Method and device for low-carbon emission energy regeneration by coupling waste gas and waste water |
| WO2015085631A1 (en) * | 2013-12-13 | 2015-06-18 | 嘉兴泽元生物制品有限责任公司 | Method for culturing botryococcus spp. with high yield |
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| CN106883982A (en) * | 2017-03-27 | 2017-06-23 | 嘉兴学院 | Pot type bioreactor and its helotisn body synchronous purification biogas biogas slurry method |
| CN107098478A (en) * | 2017-06-23 | 2017-08-29 | 四川省天惠能源科技有限公司 | A kind of microalgae carbon sequestration and sewage ecological treatment system |
| CN110218748A (en) * | 2019-06-19 | 2019-09-10 | 北京林业大学 | A method of grease is produced using microalgae low cost |
| CN110330104A (en) * | 2019-06-21 | 2019-10-15 | 临沂大学 | A method of with p-aminobenzoic acid in microorganism removal water body |
| CN110330104B (en) * | 2019-06-21 | 2022-04-29 | 临沂大学 | Method for removing p-aminobenzoic acid in water body by using microorganisms |
| CN113860511A (en) * | 2021-11-17 | 2021-12-31 | 中国矿业大学 | Intelligent microalgae water purification device and method based on porous adsorption |
| CN113860511B (en) * | 2021-11-17 | 2024-01-26 | 中国矿业大学 | Intelligent microalgae water purification device and method based on porous adsorption |
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Address after: No. 258 Wuxing Jiayuan, Liangxi District, Wuxi City, Jiangsu Province Patentee after: Jiangnan University Address before: No. 1800 road 214122 Jiangsu Lihu Binhu District City of Wuxi Province Patentee before: Jiangnan University |