CN104969908A - A rotifer high-density culture method - Google Patents
A rotifer high-density culture method Download PDFInfo
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- CN104969908A CN104969908A CN201410147372.7A CN201410147372A CN104969908A CN 104969908 A CN104969908 A CN 104969908A CN 201410147372 A CN201410147372 A CN 201410147372A CN 104969908 A CN104969908 A CN 104969908A
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- wheel animalcule
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Abstract
The present invention provides a rotifer high-density culture method including a technical scheme: seawater chlorella pyrenoidosa is used to feed rotifers with a seawater chlorella pyrenoidosa liquid concentration thrown into water body, namely a precipitation rate, of 20%-25%, a culture water body of 1500 L, a pH value controlled at 6.5-8, a dissolved oxygen concentration controlled at 3-25 mg/L, a water temperature controlled at 20-36 DEG C, and a salinity controlled at 19-30%. The control method of throwing the seawater chlorella pyrenoidosa into the water body is that intestine and stomach changes of the seawater chlorella pyrenoidosa are observed under a microscope in order to determine the application amount of the seawater chlorella pyrenoidosa. The PH value is adjusted with sodium bicarbonate or acetic acid, medium warming is performed in a circulating water way. Excretion of rotifers and residual baits are removed with hanging cloth. The culture method is reasonable and practical in design and time-saving and labor-saving. The cultured rotifer individuals are healthy and strong; and the culture method improves production efficiency and economic benefits of enterprises, and is suitable for general promotion and use.
Description
Technical field:
The present invention relates to wheel animalcule culture technique field, especially a kind of wheel animalcule high-density cultivation method.
Background technology:
Wheel animalcule is the small-sized multicellular animals of a group of rotifera door, the long 0.1-0.5mm of general body, the active bait that wheel animalcule is fry, Shrimp waste is the most agreeable to the taste, therefore in aquaculture, have quite large using value.But at present institute cultivate the method for wheel animalcule, has the dead shell of wheel animalcule many, adheres to dirt with it, sink to the bottom, torpescence, be not with ovum, dysgonic performance such as band winter egg or male body appearance etc., mainly water quality and temperature and bait input control is bad causes.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of wheel animalcule high-density cultivation method, its technical scheme is: adopt seawater chlorella pyrenoidosa to feed wheel animalcule.The concentration putting into seawater chlorella pyrenoidosa algae liquid both rate of deposition between 20-25%.
Cultivation water body is 1500L, and pH value controls between 6.5-8.Oxygen dissolved control 3 ?between 25 mg/L, water temperature controls between 20-36 DEG C, and salinity controls between 19-30 ‰.
During wheel animalcule inoculation, charge seed 7000/ml, egg laying rate of red swamp 20%, energetic, the full green of stomach, PH6.32, water temperature 34 DEG C, oxygen dissolved 17mg/L, inoculate latter 6 hours, number 7500/ml, the full green of the energetic stomach of egg laying rate of red swamp 40%, PH6.50, water temperature 34.2 DEG C, oxygen dissolved 18.6 mg/L, 12 h after inoculation, number 8500/ml, egg laying rate of red swamp 90%, energetic, the full green of stomach, PH6.35, water temperature 34.3 DEG C, oxygen dissolved 20 mg/L, inoculate latter 18 hours, number 10000/ml, egg laying rate of red swamp 80%, energetic, the full green of stomach, PH6.5, water temperature 34.6 DEG C, oxygen dissolved 23mg/L, inoculate latter 24 hours, number 14000-16000/ml, egg laying rate of red swamp 40%, energetic, stomach is yellow, PH7.20, water temperature 34.8 DEG C, oxygen dissolved 13mg/L.
Control method when seawater chlorella pyrenoidosa is thrown in examines under a microscope the change of wheel animalcule stomach, decides the usage amount of seawater chlorella pyrenoidosa with this.
Cultivating water body uses clorox or bleaching powder to carry out disinfection to water, uses after waiting the chlorion in water to volatilize completely.
The excreta of wheel animalcule and residual bait, use dossal to remove.
PH value adjustment sodium bicarbonate or acetic acid, culture fluid heats the mode adopting cycle water.
Gather with wire screen, institute uses 300 mesh sieve cloth.
The present invention's practicality reasonable in design, time saving and energy saving, the wheel animalcule cultivated is individual healthy and strong, improves enterprises production efficiency and economic benefit, is applicable to popularity and uses.
Embodiment
1, the bait of wheel animalcule
1. select the micro-algae being in exponential phase of growth, aging algae kind is unfavorable for the growth even intoxicating of wheel animalcule.2. directly in the micro-algae of high density during inoculating rotifer, ensure there is no protozoa in wheel animalcule kind, because under the abundant condition of Mirco algal food, protozoa breeds rapidly, not only wastes bait, and suppresses the growth of wheel animalcule.3. give and certain illumination the cultivation water body of wheel animalcule, the growth of micro-algae can utilize the metabolic waste of culture fluid to improve water quality.Although micro-algae optimal bait that is wheel animalcule, but the bait needed due to the mass propgation of wheel animalcule is a lot, breed wheel animalcule often can not meet need of production by cultivating micro-algae, other bait must be sought replace, and the bait that we use-seawater chlorella pyrenoidosa.
2. water quality management
The quality of water quality has close association to cultivation wheel animalcule, particularly will keep water quality to during cultivation high density wheel animalcule.
3. inflate
The effect of inflation is supplemental oxygen and prevents bait from sinking.
4. water temperature
Water temperature is the important factor affecting wheel animalcule peak period time of occurrence, and water temperature is higher, wheel animalcule peak period more early, but during High Density Cultivation wheel animalcule water temperature height have certain restriction.
5. wheel animalcule growth checks
Cultivating wheel animalcule needs through conventional microexamination, and well-grown individual loose, stomach is full, travelling active.The ratio of wheel animalcule adult band summer egg and number are the major criterions of disconnected paddle wheel worm growth, if the dead shell of wheel animalcule is many, adhere to dirt with it, sink to the bottom, torpescence, be not with ovum, and band winter egg or male body appearance etc. are all dysgonic performances.
Wheel animalcule cultivates data
1. the concentration (rate of deposition: 20-25%) of seawater chlorella pyrenoidosa algae liquid.
2. dissolved oxygen amount: 3 ?25 ㎎/L water temperature: 20-36 DEG C salinity: 19-30 ‰.
3. water body is cultivated: 1500L.
4. pH value: 6.5-8.
5. bait mode is added: use feed pump.
When 6. inoculating: charge seed 7000/ml, egg laying rate of red swamp 20% is energetic, the full green of stomach, PH6.32, water temperature 34 DEG C, oxygen dissolved 17 ㎎/L.
Inoculate latter 6 hours, number 7500/ml, the full green of the energetic stomach of egg laying rate of red swamp 40%, PH6.50, oxygen dissolved 18.6 ㎎/L, water temperature 34.2 DEG C.
12 hours: number 8500/ml, egg laying rate of red swamp 90%, energetic, the full green of stomach, PH6.35, oxygen dissolved 20 ㎎/L, water temperature 34.3 DEG C.
18 hours: number 10000/ml, egg laying rate of red swamp 80%, energetic, the full green of stomach, PH6.5, oxygen dissolved 23 ㎎/L, water temperature 34.6 DEG C.
24 hours: number 14000-16000/ml, egg laying rate of red swamp 40%, energetic, stomach is yellow, PH7.20, oxygen dissolved 13 ㎎/L, water temperature 34.8 DEG C.
7. chlorella throws in period control method
Examine under a microscope the change of wheel animalcule stomach, normal color is green, can reduce the usage amount of bait like this.
8. foam color and size variation is observed
9. other points for attention
Cultivate water body to use by strict sterilization or after filtering, if when using clorox or bleaching powder to carry out disinfection to water, the chlorion in water must be waited to volatilize completely could be used; High Density Cultivation wheel animalcule, culture fluid keeps away unavoidable pollution, because have excreta and the residual bait of wheel animalcule, these need by using dossal to remove, otherwise ammonia poisoning can be caused to affect cultivation; Ph regulates available sodium bicarbonate and acetic acid; Culture fluid heats the mode that can adopt cycle water, and directly heating with heating rod affects culture density; Clear cylinder is wanted thoroughly, to make without protist in cylinder body, can use if desired with after hypochlorite disinfectant; Ensure that culture fluid bait is sufficient, PH is constant, and dissolved oxygen amount is sufficient, and water quality is good, can add new water if desired; Used wire screen of gathering is 300 orders, and complete clear water of gathering rinses one time, then renders in fresh water body.
Other wheel animalcule culture technique index and data
1. judge that in water body, whether ammonium taste is excessive by sense of smell or employing instrument, should inverted engine at once if ammonium taste is excessive.2. by the vigor of microscope close observation wheel animalcule, stomach color, protect ovum rate, young quantity, wheel animalcule dead volume quantity (too much will analyze the cause of the death), have microorganism impurity density in (Zoothamnium, campanularian, scratch the same clan etc.), water body, food density, with or without bacteriological infection.3. the color, size and the product foam amount that produce foam is observed.
(i) foam color: glossy dark green mulberry.(ii) foam be air pocket (if the many foams of bubble little be because oxygen input quantity excessive).(iii) produce foam amount large, majority can be excessive outside container.4. in water body, impurity too much can adopt linked network (cotton cloth) mode plug-in, cleaning-clorox dilution water body soak 5 minutes-clean-dry-reuse.5. wheel animalcule young number speak more water pollution that the low explanation of bright well-grown ﹔ larva number likely occurs, bait long-time deficient, oxygen content is too low or too high, microbial contamination, bacteriological infection etc. produce that resting egg causes.6. stomach color judge daily ration, feeding quantity (green best, light green color and yellow for next can bait throwing in, yellowly want horse back bait throwing in).7. microorganism too much (Zoothamnium, campanularian, scratch the same clan), inverted engine at once (first gets rid of container stratum water body before inverted engine, with cotton cloth filter impurities in water, little wheel animalcule at present face 260 orders filter cloth ∕ large wheel animalcule be below with 200 order filter-cloth filterings, filter the equal salinity cleaning and dipping of the equal water temperature of complete use, spare water body is put in three postocciput repeatedly, examines.8. the too low available sodium bicarbonate of PH change regulates; Cross High Availabitity 0.1N hydrochloric acid solution to regulate.9. number and Production rate mode is counted: 1ml suction pipe can be used when charging seed, when density is excessive, 0.1ml suction pipe can be used, no matter all need average from several number of counting of container internal fixtion with great suction pipe.Account form: Li is as ﹕ 30 Ge ∕ ml × 1000 Sheng ﹙ water body Liang ﹚ × 3 μ g=0.09kg, 1g=1000000 μ g.10. the packing of product: hold with double-layer plastic bag, throws in wheel animalcule 6-9 hundred million, water 3-5 ㎏ of equal salinity, puts into bubble chamber after pouring purity oxygen sealing.
Wheel animalcule cultivation water: can adopt Xiao Du Shui ﹙ Lv Suan Na ﹚ or get ready in advance with Piao Bai Fen ﹙, water body Tou Che ﹚ must treat chlorine San Jing ﹚ to reduce before protozoic Wu Ran ﹙ uses, and adopts high density Lv Bu ﹙ 0.1 μ m ﹚ to filter.
Embodiment: the water yield 1500 L, water temperature 33-34 DEG C, bait is 50% concentrated chlorella, incubation time 24 hours, and oxygen supply is liquid oxygen, and organic matter removal adopts air foaming and linked network to filter; Throw seedling 3000/ml, i.e. 4,500,000,000/cylinder, close 7.425 kilograms (proportion 165g/ hundred million), density of gathering 10000/ml, i.e. 15,000,000,000/cylinder, close 24.75 kilograms, net production 17.325 kilograms, bait consumes 30 kilograms, feed coefficient 1 ︰ 1.73.
Below be only exemplary embodiments of the present invention; be not intended to limit the present invention; in protection scope of the present invention; those skilled in the art can carry out suitable adjustment to each controling parameters completely according to actual needs; but identical with the principle of the various embodiments described above, all fall within protection scope of the present invention.
Claims (10)
1. a wheel animalcule high-density cultivation method, is characterized in that: adopt seawater chlorella pyrenoidosa to feed wheel animalcule.
2. a kind of wheel animalcule high-density cultivation method according to claim 1, is characterized in that: the concentration putting into seawater chlorella pyrenoidosa algae liquid both rate of deposition between 20-25%.
3. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, is characterized in that: cultivation water body is 1500L, and pH value controls between 6.5-8.
4. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, is characterized in that: oxygen dissolved control 3 ?between 25 mg/L, water temperature controls between 20-36 DEG C, and salinity controls between 19-30 ‰.
5. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, it is characterized in that, during wheel animalcule inoculation, charge seed 7000/ml, egg laying rate of red swamp 20%, energetic, the full green of stomach, PH6.32, water temperature 34 DEG C, oxygen dissolved 17mg/L, inoculate latter 6 hours, number 7500/ml, egg laying rate of red swamp 40% is energetic, the full green of stomach, PH6.50, water temperature 34.2 DEG C, oxygen dissolved 18.6 mg/L, 12 h after inoculation, number 8500/ml, egg laying rate of red swamp 90%, energetic, the full green of stomach, PH6.35, water temperature 34.3 DEG C, oxygen dissolved 20 mg/L, inoculate latter 18 hours, number 10000/ml, egg laying rate of red swamp 80%, energetic, the full green of stomach, PH6.5, water temperature 34.6 DEG C, oxygen dissolved 23mg/L, inoculate latter 24 hours, number 14000-16000/ml, egg laying rate of red swamp 40%, energetic, stomach is yellow, PH7.20, water temperature 34.8 DEG C, oxygen dissolved 13mg/L.
6. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, is characterized in that, control method when seawater chlorella pyrenoidosa is thrown in examines under a microscope the change of wheel animalcule stomach, decides the usage amount of seawater chlorella pyrenoidosa with this.
7. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, is characterized in that, cultivates water body and uses clorox or bleaching powder to carry out disinfection to water, use after waiting the chlorion in water to volatilize completely.
8. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, is characterized in that: the excreta of wheel animalcule and residual bait, uses dossal to remove.
9. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, pH value adjustment sodium bicarbonate or acetic acid, culture fluid heats the mode adopting cycle water.
10. a kind of wheel animalcule high-density cultivation method according to claim 1 and 2, is characterized in that: gather with wire screen, and institute uses 300 mesh sieve cloth.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230577A (en) * | 2015-11-30 | 2016-01-13 | 江苏纳克生物工程有限公司 | Continuous culturing method for brachionus calyciflorus |
| CN105284742A (en) * | 2015-11-16 | 2016-02-03 | 中国水产科学研究院淡水渔业研究中心 | Rotifer continuous culturing system and rotifer continuous culturing method |
| CN112772478A (en) * | 2021-01-12 | 2021-05-11 | 曹礼刚 | Culture method of rotifers |
Citations (2)
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| CN1989803A (en) * | 2005-12-26 | 2007-07-04 | 中国水产科学研究院东海水产研究所 | Method for killing copepods in rotaria culture |
| CN102630645A (en) * | 2012-04-23 | 2012-08-15 | 天津海友佳音生物科技股份有限公司 | Method for culturing ss (super-small) type branchionus plicatilis by utilizing high-concentration fresh water chlorella |
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2014
- 2014-04-14 CN CN201410147372.7A patent/CN104969908A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1989803A (en) * | 2005-12-26 | 2007-07-04 | 中国水产科学研究院东海水产研究所 | Method for killing copepods in rotaria culture |
| CN102630645A (en) * | 2012-04-23 | 2012-08-15 | 天津海友佳音生物科技股份有限公司 | Method for culturing ss (super-small) type branchionus plicatilis by utilizing high-concentration fresh water chlorella |
Non-Patent Citations (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284742A (en) * | 2015-11-16 | 2016-02-03 | 中国水产科学研究院淡水渔业研究中心 | Rotifer continuous culturing system and rotifer continuous culturing method |
| CN105230577A (en) * | 2015-11-30 | 2016-01-13 | 江苏纳克生物工程有限公司 | Continuous culturing method for brachionus calyciflorus |
| CN105230577B (en) * | 2015-11-30 | 2018-03-30 | 江苏纳克生物工程有限公司 | The continuous cultural method of Brachionus calyciflorus |
| CN112772478A (en) * | 2021-01-12 | 2021-05-11 | 曹礼刚 | Culture method of rotifers |
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