CN110326630A - A method of biological pesticide is prepared using tomato stem-leaf - Google Patents

A method of biological pesticide is prepared using tomato stem-leaf Download PDF

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Publication number
CN110326630A
CN110326630A CN201910650867.4A CN201910650867A CN110326630A CN 110326630 A CN110326630 A CN 110326630A CN 201910650867 A CN201910650867 A CN 201910650867A CN 110326630 A CN110326630 A CN 110326630A
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CN
China
Prior art keywords
leaf
tomato stem
biological pesticide
fermentation
tomato
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Pending
Application number
CN201910650867.4A
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Chinese (zh)
Inventor
薛萍
史俊卿
付宏岐
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Weinan Vocational & Technical College
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Weinan Vocational & Technical College
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Priority to CN201910650867.4A priority Critical patent/CN110326630A/en
Publication of CN110326630A publication Critical patent/CN110326630A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/38Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

A kind of method preparing biological pesticide using tomato stem-leaf provided by the invention, which is characterized in that the described method comprises the following steps: raw material preparation, fermentation, fermentation liquid post-processing.The beneficial effects of the present invention are: waste-tomato stem-leaf using tomato planting is raw material, without material is dry, crushing, is extracted, concentration, but fermentation liquid stoste is sprayed directly on to plant, is used for desinsection, antibacterial and plant protection;Meanwhile it is more efficient than directly utilizing water, alcohol, sour water to extract using fermentation extraction tomatidine;The acid condition of fermentation liquid makes tomatin be converted into tomatidine, and toxicity is smaller;Sugar without rejecting, can be used as plant nutrient agent and adhesive, helping medicament attachment, prevent moisture from evaporating after fermentation;In addition, fermentation reaction mild condition, saves man power and material, alcohol and acid is added without additional, reduces the cost, and no pollution to the environment.

Description

A method of biological pesticide is prepared using tomato stem-leaf
Technical field
The invention belongs to technical field of biochemical industry, be related to a kind of biological insecticides, bacteriostatic agent and plant protection product and its Preparation method, and in particular to a method of biological pesticide is prepared using tomato stem-leaf.
Background technique
Currently, China's chemical pesticide causes Practice for Pesticide Residue in Agricultural Products exceeded, remaining pesticide is to human body and ring using frequently Border brings very big harm, while also limiting the outlet of agricultural product.With the enhancing of health of people and environmental consciousness, to organic The demand of food and green non-pollution food is also higher and higher, this allows for naturally extraction, mild, nontoxic plant source biology agriculture Medicine becomes the preferred product of people, but the type of selectable biological pesticide is considerably less currently on the market, is badly in need of new Efficiently, nontoxic, safe and environment-friendly biological pesticide.
In addition, traditional botanical biological pesticide preparation process is complicated, consumes a large amount of manpowers and the energy, while also to environment Cause burden and pollution.Generally first plant to be dried, be clayed into power, then is molten to extract with water, methanol or ethyl alcohol etc. Agent, is suitably equipped with acid or alkali extracts, and is re-dissolved again with solvent appropriate after recycling design, is configured to debita spissitudo It uses.Therefore, a kind of preparation method of novel botanical biological pesticide is needed to solve system present in existing technology of preparing The problems such as standby process is complicated, resource utilization is low, environmental pollution is big.
Summary of the invention
It is of the existing technology in order to solve the problems, such as, biological pesticide is prepared using tomato stem-leaf the present invention provides a kind of Method, the method reaction condition is mild, saves man power and material, alcohol and acid is added without additional, production cost is low, environmental pollution It is small.
A method of biological pesticide is prepared using tomato stem-leaf, comprising the following steps:
(1) raw material preparation: preparing a certain amount of tomato stem-leaf tissue, is shredded stand-by;In addition prepare suitable brown sugar, Yeast and water;Aforementioned tomato stem-leaf bar tissue can be dried plant tissue, or fresh plant tissue;
(2) ferment: weigh the aforementioned tomato stem-leaf tissue after shredding, brown sugar, yeast 1~6:1 in mass ratio~6:0.1~ 1.0% is put into glass jar, adds the water of 8~20 times of aforementioned tomato stem-leaf liver masses, mixes and floats face waterborne Tissue is pressed under liquid level, is closed the lid, and fermentation 30 days is protected from light;The glass jar is shaken in first 3 days of aforesaid fermentation process daily, protects Raw material is demonstrate,proved to be uniformly mixed;And entire fermentation process keeps aforementioned tomato stem-leaf tissue to be in below liquid level;
(3) fermentation liquid post-processes: after aforesaid fermentation process, with filtered on buchner funnel fermentation liquid, filtrate is to utilize The biological pesticide of tomato stem-leaf preparation.
As a preferred solution, tomato stem-leaf tissue, brown sugar, yeast, water after being shredded described in step (2) plus Entering amount is respectively 300 grams, 300 grams, 0.1 gram, 3 liters.
It is further preferred that the additional amount of tomato stem-leaf tissue, brown sugar, yeast, water after being shredded described in step (2) point It Wei not be 150 grams, 300 grams, 0.5 gram, 3 liters.
It is further preferred that yeast described in step (2) is saccharomyces cerevisiae.
It is a kind of using tomato stem-leaf preparation biological pesticide be prepared using method above-mentioned.
A kind of biological pesticide using tomato stem-leaf preparation above-mentioned is used for the prevention and treatment of aphid, and the rear aphid death rate can for 24 hours To reach 95% or more.
A kind of biological pesticide using tomato stem-leaf preparation above-mentioned is used for the prevention and treatment of fruit white rot of grape and anthrachose of grape, prevention and treatment Rate can achieve 85% or more.
The beneficial effects of the present invention are: a kind of method tool preparing biological pesticide using tomato stem-leaf provided by the invention There is following advantage:
(1) preparation method is simple: entire preparation method is simple, and formula mixes in proportion, and gained stoste can be direct Medication;
(2) efficiently: yeasting is especially suitable for alkaloids substance, and extraction efficiency is high;
(3) economical: tomato stem-leaf is originally the waste in tomato production, is recycled, and production cost is low;
(4) environmentally friendly: biological pesticide does not use any chemical reagents during the preparation process, sprays that process is nontoxic, safety, preparation It is non-hazardous to environment with use process;
(5) strong operability, easy to spread: production without special instrument and equipment, method is simple, condition is easily-controllable, very Suit large area to popularize use;
(6) overall advantage: waste-tomato stem-leaf using tomato planting is raw material, without material is dry, crush, It extracts, concentration, but fermentation liquid stoste is sprayed directly on to plant, be used for desinsection, antibacterial and plant protection;Meanwhile utilizing fermentation It is more efficient than directly utilizing water, alcohol, sour water to extract to extract tomatidine;The acid condition of fermentation liquid is converted into tomatin kind Solanine, toxicity are smaller;Sugar without rejecting, can be used as plant nutrient agent and adhesive, helping medicament attachment, prevent after fermentation Sealing point evaporation;In addition, fermentation reaction mild condition, saves man power and material, alcohol and acid is added without additional, reduces the cost, And no pollution to the environment.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, to the technology in the embodiment of the present invention Scheme is clearly and completely described, it is clear that and described embodiments are some of the embodiments of the present invention, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work The every other embodiment obtained, shall fall within the protection scope of the present invention.
Embodiment 1
It is a kind of using tomato stem-leaf prepare biological pesticide method the following steps are included:
(1) raw material preparation: prepare the dry tissue of a certain amount of tomato stem-leaf, shredded stand-by;In addition prepare suitable Brown sugar, yeast and water;
(2) ferment: weigh the dry tissue of the tomato stem-leaf after shredding, brown sugar, saccharomyces cerevisiae, water additional amount be respectively 300 grams, 300 grams, 0.1 gram are put into glass jar, add 3 liters of water, mix and the tissue that floats face waterborne is by under liquid level, It closes the lid, is protected from light fermentation 30 days;The glass jar is shaken in first 3 days of fermentation process daily, guarantees that raw material is uniformly mixed;And it is whole A fermentation process keeps the dry tissue of the tomato stem-leaf to be in below liquid level;
(3) fermentation liquid post-processes: after fermentation process, with filtered on buchner funnel fermentation liquid, filtrate is to utilize tomato The biological pesticide of cauline leaf preparation;
(4) control efficiency is evaluated: filtrate is uniformly sprayed on the blade of aphid, and rear aphid almost all is dead for 24 hours, the death rate 95% or more.
Embodiment 2
It is a kind of using tomato stem-leaf prepare biological pesticide method the following steps are included:
(1) raw material preparation: prepare the dry tissue of a certain amount of tomato stem-leaf, shredded stand-by;In addition prepare suitable Brown sugar, yeast and water;
(2) ferment: weigh the dry tissue of the tomato stem-leaf after shredding, brown sugar, yeast, water additional amount be respectively 150 grams, 300 grams, 0.5 gram are put into glass jar, add 3 liters of water, mix and the tissue that floats face waterborne is by under liquid level, close the lid Son is protected from light fermentation 30 days;The glass jar is shaken in first 3 days of fermentation process daily, guarantees that raw material is uniformly mixed;And entire fermentation Process keeps the dry tissue of the tomato stem-leaf to be in below liquid level;
(3) fermentation liquid post-processes: after the fermentation process, with filtered on buchner funnel fermentation liquid, filtrate is to utilize The biological pesticide of tomato stem-leaf preparation;
(4) control efficiency is evaluated: filtrate is uniformly sprayed on grape leave and fruit, sprays 1 every 10~15 days in the season of growth It is secondary, it need to spray altogether 4~5 times, very good to the control efficiency of fruit white rot of grape and anthrachose of grape, control rate can achieve 85% or more.
It should be appreciated that described above, the specific embodiments are only for explaining the present invention, is not intended to limit the present invention.By The obvious changes or variations that spirit of the invention is extended out are still in the protection scope of this invention.

Claims (7)

1. a kind of method for preparing biological pesticide using tomato stem-leaf, which is characterized in that the described method comprises the following steps:
(1) raw material preparation: preparing a certain amount of tomato stem-leaf tissue, is shredded stand-by;In addition prepare suitable brown sugar, yeast And water;The tomato stem-leaf bar tissue can be dried plant tissue, or fresh plant tissue;
(2) ferment: weigh the tomato stem-leaf tissue after shredding, brown sugar, yeast 1~6:1 in mass ratio~6:0.1~ 1.0% is put into glass jar, adds the water of 8~20 times of tomato stem-leaf liver masses, mixes and floats face waterborne Tissue is pressed under liquid level, is closed the lid, and fermentation 30 days is protected from light;The glass jar is shaken in first 3 days of the fermentation process daily, protects Raw material is demonstrate,proved to be uniformly mixed;And entire fermentation process keeps the tomato stem-leaf tissue to be in below liquid level;
(3) fermentation liquid post-processes: after the fermentation process, with filtered on buchner funnel fermentation liquid, filtrate is to utilize tomato The biological pesticide of cauline leaf preparation.
2. a kind of method for preparing biological pesticide using tomato stem-leaf according to claim 1, which is characterized in that step (2) additional amount of tomato stem-leaf tissue, brown sugar, yeast, water after shredding described in is respectively 300 grams, 300 grams, 0.1 gram, 3 It rises.
3. a kind of method for preparing biological pesticide using tomato stem-leaf according to claim 1, which is characterized in that step (2) additional amount of tomato stem-leaf tissue, brown sugar, yeast, water after shredding described in is respectively 150 grams, 300 grams, 0.5 gram, 3 It rises.
4. a kind of method for preparing biological pesticide using tomato stem-leaf according to claim 2, which is characterized in that step (2) yeast described in is saccharomyces cerevisiae.
5. a kind of biological pesticide prepared using tomato stem-leaf, which is characterized in that the biological pesticide is using claim 1-4 What the method was prepared.
6. a kind of biological pesticide prepared using tomato stem-leaf according to claim 5, which is characterized in that the biology agriculture Medicine is used for the prevention and treatment of aphid.
7. a kind of biological pesticide prepared using tomato stem-leaf according to claim 5, which is characterized in that the biology agriculture Medicine is used for the prevention and treatment of fruit white rot of grape and anthrachose of grape.
CN201910650867.4A 2019-07-18 2019-07-18 A method of biological pesticide is prepared using tomato stem-leaf Pending CN110326630A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111448957A (en) * 2020-04-26 2020-07-28 江苏德得健康产业有限公司 Organic planting method for cherry tomatoes rich in organic selenium

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Publication number Priority date Publication date Assignee Title
CN111448957A (en) * 2020-04-26 2020-07-28 江苏德得健康产业有限公司 Organic planting method for cherry tomatoes rich in organic selenium

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