CN110301558A - A kind of microcapsules fish feed additive and preparation method thereof rich in natural astaxanthin - Google Patents
A kind of microcapsules fish feed additive and preparation method thereof rich in natural astaxanthin Download PDFInfo
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- CN110301558A CN110301558A CN201910719945.1A CN201910719945A CN110301558A CN 110301558 A CN110301558 A CN 110301558A CN 201910719945 A CN201910719945 A CN 201910719945A CN 110301558 A CN110301558 A CN 110301558A
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- microcapsules
- rich
- natural astaxanthin
- astaxanthin
- feed additive
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- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 74
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims abstract description 73
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims abstract description 73
- 229940022405 astaxanthin Drugs 0.000 title claims abstract description 73
- 239000001168 astaxanthin Substances 0.000 title claims abstract description 73
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 49
- 239000003094 microcapsule Substances 0.000 title claims abstract description 39
- 239000003674 animal food additive Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 20
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 20
- 239000000661 sodium alginate Substances 0.000 claims abstract description 20
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 20
- 108010010803 Gelatin Proteins 0.000 claims abstract description 19
- 239000008273 gelatin Substances 0.000 claims abstract description 19
- 229920000159 gelatin Polymers 0.000 claims abstract description 19
- 235000019322 gelatine Nutrition 0.000 claims abstract description 19
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 15
- 239000000243 solution Substances 0.000 claims abstract description 14
- 239000011259 mixed solution Substances 0.000 claims abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000000725 suspension Substances 0.000 claims abstract description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 5
- 241000195493 Cryptophyta Species 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 5
- 239000002775 capsule Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 239000000796 flavoring agent Substances 0.000 claims description 6
- 235000019634 flavors Nutrition 0.000 claims description 6
- 230000036039 immunity Effects 0.000 claims description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 6
- 241000238557 Decapoda Species 0.000 claims description 5
- 235000019441 ethanol Nutrition 0.000 claims description 4
- 238000007605 air drying Methods 0.000 claims description 3
- 238000000265 homogenisation Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000002525 ultrasonication Methods 0.000 claims description 3
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 239000000284 extract Substances 0.000 abstract description 5
- 238000009360 aquaculture Methods 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 241000168517 Haematococcus lacustris Species 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 241000081271 Phaffia rhodozyma Species 0.000 description 2
- 150000001514 astaxanthins Chemical class 0.000 description 2
- 239000011805 ball Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000003760 magnetic stirring Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000195627 Chlamydomonadales Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000168525 Haematococcus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002730 additional effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005354 coacervation Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a kind of microcapsules fish feed additive and preparation method thereof rich in natural astaxanthin, belongs to fish feed additive technology field.Technical solution of the present invention main points are as follows: a kind of microcapsules fish feed addictive preparation method rich in natural astaxanthin, specific steps are as follows: extract the natural astaxanthin in algae powder with n-hexane-ethyl alcohol mix reagent and obtain natural astaxanthin extracting solution；Natural astaxanthin extracting solution is added to mixing homogenate in gelatin and mixed solution of sodium alginate again and obtains astaxanthin mixing suspension；Then astaxanthin mixing suspension is added dropwise in calcium chloride solution, which agglomerates it again, forms microcapsules, and the microcapsules fish feed additive of natural astaxanthin is finally rich in through being dried to obtain.Microcapsules fish feed additive produced by the present invention rich in natural astaxanthin can effectively improve fish quality, improve efficiency of feed utilization, reduce aquaculture cost.
Description
Technical field
The invention belongs to fish feed additive technology fields, and in particular to a kind of microcapsules fish rich in natural astaxanthin
Class feed addictive and preparation method thereof.
Background technique
Haematococcus pluvialis belongs to volvocales, and haematococcus section is widely present in nature, environment be suitable for, nutritional sufficiency
Water body in, haematococcus pluvialis is in long travelling state amphitrichous and starts to be converted into without motion once environmental condition is unfavorable
The glue sheath of ability, and then it is converted into the red sporangiocyst state with sclereid wall.Haematococcus pluvialis accumulates in the conversion process
Tire out a large amount of astaxanthins, the haematococcus pluvialis of red sporangiocyst state is the best source of natural astaxanthin.
Natural astaxanthin is to be currently known a kind of extremely strong substance of oxidation resistance, and oxidation resistance is ascorbic
6000 times, there are three types of the sources for being currently known natural astaxanthin, a fungi fermentation (red phaffia rhodozyma), two aquatic products (shrimp, crab
Class) waste extraction, the extraction of three haematococcus pluvialis.Haematococcus pluvialis becomes the best source of astaxanthin with its high yield, naturally
Astaxanthin is widely used in aquaculture, and aquatic products immunity can be improved, and enhances its disease resistance；Aquatic products body can be improved
Color makes to have coloured animal body colour more bright-coloured；Aquatic products reproductive capacity can be enhanced, improve production level；For fish culture
In, flesh of fish flavor can be improved, make its mouthfeel more preferably.
Summary of the invention
The system of one of the objects of the present invention is to provide a kind of microcapsules fish feed additive rich in natural astaxanthin
Preparation Method, this method is easy to operate, can effectively extract astaxanthin contained by algae powder, solves astaxanthin status not easy to maintain, mentions
The utilization efficiency of high astaxanthin.
The second object of the present invention is to provide a kind of microcapsules fish feed additive rich in natural astaxanthin, this is micro-
Capsule fish feed additive is prepared by the above method, is rich in natural astaxanthin, be can effectively improve fish immunity power, is improved
Body colour improves flesh of fish flavor.
The present invention adopts the following technical scheme that achieve the above object, and a kind of microcapsules fish rich in natural astaxanthin are raised
The preparation method of feed additives, it is characterised in that specific steps are as follows: extracted with n-hexane-ethyl alcohol mix reagent natural in algae powder
Astaxanthin obtains natural astaxanthin extracting solution；Natural astaxanthin extracting solution is added in gelatin and mixed solution of sodium alginate again
Mixing homogenate obtains astaxanthin mixing suspension；Then astaxanthin mixing suspension is added dropwise in calcium chloride solution makes it
Multiple cohesion forms microcapsules, and the microcapsules fish feed additive of natural astaxanthin, the microcapsules are finally rich in through being dried to obtain
Fish feed additive is spherical capsule pattern, and natural astaxanthin is embedded in spherical capsule, and wall material is gelatin and sodium alginate, ball
The particle diameter of shape capsule is 1-2mm.
Preferably, the volume ratio of n-hexane and ethyl alcohol is 1:1 in the n-hexane-ethyl alcohol mix reagent, in extraction process
It is complete that ultrasonication helps extraction.
Preferably, the weight ratio of gelatin and sodium alginate is 1:2 in the gelatin and mixed solution of sodium alginate.
Preferably, the weight ratio of the natural astaxanthin extracting solution and gelatin and mixed solution of sodium alginate is 1:10, is led to
It crosses magnetic stirring apparatus to help dissolve, mixing Homogenization time is 30min.
Preferably, the multiple pool time is 30min.
Preferably, described dry dry using cold air drying or drier.
Microcapsules fish feed additive of the present invention rich in natural astaxanthin, it is characterised in that be by above-mentioned side
What method was prepared, which is rich in natural astaxanthin, for being effectively improved fish immunity power, improving
Body colour and improvement flesh of fish flavor.
Preparation process of the present invention is easy to operate, final that the microcapsules fish feed additive rich in natural astaxanthin is made,
Natural astaxanthin has extremely strong oxidation resistance, is added in aquatic animal feed, can enhance aquatic livestock immunity, improve
Fertility, increase flavor, blast body colour etc., the microcapsules fish feed additive produced by the present invention rich in natural astaxanthin
Fish quality can be effectively improved, efficiency of feed utilization is improved, reduces aquaculture cost.
Specific embodiment
To make the purpose of the embodiment of the present invention, technical solution and clearer, day is rich in the embodiment of the present invention below
The technical solution of the microcapsules fish feed additive of right astaxanthin and preparation method thereof is clearly and completely described.
Microcapsules fish feed additive provided in an embodiment of the present invention rich in natural astaxanthin, is made an addition to fish
In feed, astaxanthin can be effectively improved in the intracorporal absorptivity of fish, to effectively improve the food ration and immunity of fish, together
When can also effectively improve fish body colour and flesh of fish flavor.
Mesichthyes feed addictive of the embodiment of the present invention mainly contains natural astaxanthin.The main source of natural astaxanthin has
It is extracted in the aquatic products waste such as three kinds: one shrimps, crabs, two Mycophyta cultures (red phaffia rhodozyma) production, the training of three haematococcus pluvialis
It supports and extracts.In the actual production process, haematococcus pluvialis is the best source of natural astaxanthin, the high nutritive value of astaxanthin and
Use value is all very beneficial to fish growth, breeding.
Sporinite because of haematococcus pluvialis rich in astaxanthin has thick and hard cell wall, it is difficult to directly by fish institute
It absorbs, therefore the present invention extracts natural astaxanthin during the preparation process, while with gelatin-digestible material of sodium alginate animal body
Material is used as embedding medium, and astaxanthin embed as capsule shape, is easy to also solve astaxanthin appearance not easy to maintain while fish body absorption
Problem of easy degradation.
The preparation method of the above-mentioned microcapsules fish additive rich in natural astaxanthin includes the following steps: with n-hexane-
The natural astaxanthin that ethyl alcohol mix reagent extracts in algae powder obtains natural astaxanthin extracting solution, the n-hexane-ethyl alcohol mix reagent
The volume ratio of middle n-hexane and ethyl alcohol is 1:1, and it is complete to help extraction for ultrasonication in extraction process；Natural astaxanthin is extracted again
Liquid is added to mixing homogenate in gelatin and mixed solution of sodium alginate and obtains astaxanthin mixing suspension, and wherein natural astaxanthin mentions
Taking the weight ratio of liquid and gelatin and mixed solution of sodium alginate is 1:10, helps dissolve by magnetic stirring apparatus, when mixing is homogenized
Between be 30min, the weight ratio of gelatin and sodium alginate is 1:2 in the gelatin and mixed solution of sodium alginate；Then by astaxanthin
Mixing suspension, which is added dropwise in calcium chloride solution, makes it agglomerate 30min formation microcapsules again, is finally rich in through being dried to obtain
The microcapsules fish feed additive of natural astaxanthin, dry to be spontaneously dried using cold air drying or drier, the microcapsules
Fish feed additive is spherical capsule pattern, and natural astaxanthin is embedded in spherical capsule, and wall material is gelatin and sodium alginate, ball
The particle diameter of shape capsule is 1-2mm.
Specifically, this experiment is using gelatin, sodium alginate, calcium chloride as research object, using L9(3)4Orthogonal test, with micro-
Encapsulated yield is evaluation index, even in the optimum proportioning and experimentation of wall material ratio, core wall ratio during Selection experiment
Slurry agglomerates the optimization process time again.
1 factor level table of table
Orthogonal experiments are as shown in table 2, and the primary and secondary sequence for influencing the factor of the yield of microcapsules is B > A > D > C, by putting down
Mean value is as it can be seen that the strong and weak sequence that each factor level influences result is A2>A3>A1, B1>B2>B3, C3>C2>C1, D3>D2>D1, therefore
Determine that optimum process condition is A2B1C3D3, i.e. wall material ratio is 1:2 (weight ratio of gelatin and sodium alginate is 1:2), core wall ratio
Example is 1:10 (weight ratio of natural astaxanthin extracting solution and gelatin and mixed solution of sodium alginate is 1:10), mixes Homogenization time
For 30min, it is 88.90% that multiple pool time, which is 30min, with this condition the yield of microcapsules highest.
2 Orthogonal experiment results of table
Above-mentioned n-hexane-ethyl alcohol mix reagent is provided according to FAO, can be used in food industry, can effectively be crushed frustule,
Determination of Astaxanthin in Haematococcus Pluvialis is set sufficiently to discharge.Optionally, above-mentioned frustule is crushed extraction process, can be subject to ultrasound
Addi-tional crusher, optimum condition are as follows: time 5min, power 350W, pulse: 5s, 3s.Above-mentioned multiple coacervation process, can be subject to magnetic force
Blender auxiliary dissolution, makes it sufficiently condense glomeration microcapsules.
In conclusion the preparation side of the present invention provides a kind of microcapsules fish feed additive rich in natural astaxanthin
Method, a specific embodiment of the invention describe the prefered embodiments of the present invention in detail, and under according to the principle of the invention, make
Any simple modification, equivalent variations and change out, still fall within the protection scope of technical solution of the present invention.
Claims (7)
1. a kind of preparation method of the microcapsules fish feed additive rich in natural astaxanthin, it is characterised in that specific steps
Are as follows: the natural astaxanthin in algae powder, which is extracted, with n-hexane-ethyl alcohol mix reagent obtains natural astaxanthin extracting solution；Again by natural shrimp
Green element extracting solution is added to mixing homogenate in gelatin and mixed solution of sodium alginate and obtains astaxanthin mixing suspension；Then by shrimp
Green element mixing suspension, which is added dropwise in calcium chloride solution, agglomerate it again to form microcapsules, be finally dried to obtain and be rich in day
The microcapsules fish feed additive of right astaxanthin, the microcapsules fish feed additive are spherical capsule pattern, spherical capsule
Interior embedding natural astaxanthin, wall material are gelatin and sodium alginate, and the particle diameter of spherical capsule is 1-2mm.
2. the preparation method of the microcapsules fish feed additive according to claim 1 rich in natural astaxanthin, special
Sign is: the volume ratio of n-hexane and ethyl alcohol is 1:1, ultrasonication in extraction process in the n-hexane-ethyl alcohol mix reagent
It is complete to help extraction.
3. the preparation method of the microcapsules fish feed additive according to claim 1 rich in natural astaxanthin, special
Sign is: the weight ratio of gelatin and sodium alginate is 1:2 in the gelatin and mixed solution of sodium alginate.
4. the preparation method of the microcapsules fish feed additive according to claim 1 rich in natural astaxanthin, special
Sign is: the weight ratio of the natural astaxanthin extracting solution and gelatin and mixed solution of sodium alginate is 1:10, is stirred by magnetic force
It mixes device to help dissolve, mixing Homogenization time is 30min.
5. the preparation method of the microcapsules fish feed additive according to claim 1 rich in natural astaxanthin, special
Sign is: the multiple pool time is 30min.
6. the preparation method of the microcapsules fish feed additive according to claim 1 rich in natural astaxanthin, special
Sign is: described dry dry using cold air drying or drier.
7. the microcapsules fish feed obtained for being rich in natural astaxanthin of method described in any one of -6 according to claim 1
Additive, the microcapsules fish feed additive be rich in natural astaxanthin, for be effectively improved fish immunity power, improve body colour and
Improve flesh of fish flavor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910719945.1A CN110301558A (en) | 2019-08-06 | 2019-08-06 | A kind of microcapsules fish feed additive and preparation method thereof rich in natural astaxanthin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910719945.1A CN110301558A (en) | 2019-08-06 | 2019-08-06 | A kind of microcapsules fish feed additive and preparation method thereof rich in natural astaxanthin |
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| Publication Number | Publication Date |
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| CN110301558A true CN110301558A (en) | 2019-10-08 |
Family
ID=68083136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910719945.1A Pending CN110301558A (en) | 2019-08-06 | 2019-08-06 | A kind of microcapsules fish feed additive and preparation method thereof rich in natural astaxanthin |
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| Country | Link |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110604249A (en) * | 2019-10-25 | 2019-12-24 | 常熟理工学院 | Astaxanthin-containing microcapsule effervescent tablet and preparation method thereof |
| CN111184870A (en) * | 2019-10-23 | 2020-05-22 | 浙江海洋大学 | Preparation method of astaxanthin transporter with gastric juice digestion resistance |
| CN112205523A (en) * | 2020-10-30 | 2021-01-12 | 南京卓蓝生物有限公司 | Feed rich in natural astaxanthin and preparation method thereof |
| CN115316549A (en) * | 2022-08-25 | 2022-11-11 | 李雄 | Feed additive for improving reproductive capacity of cocks and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111184870A (en) * | 2019-10-23 | 2020-05-22 | 浙江海洋大学 | Preparation method of astaxanthin transporter with gastric juice digestion resistance |
| CN110604249A (en) * | 2019-10-25 | 2019-12-24 | 常熟理工学院 | Astaxanthin-containing microcapsule effervescent tablet and preparation method thereof |
| CN112205523A (en) * | 2020-10-30 | 2021-01-12 | 南京卓蓝生物有限公司 | Feed rich in natural astaxanthin and preparation method thereof |
| CN115316549A (en) * | 2022-08-25 | 2022-11-11 | 李雄 | Feed additive for improving reproductive capacity of cocks and preparation method thereof |
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Application publication date: 20191008 |