CN110257298A - A kind of microorganism mixed bacterial promoting photosynthesis of plant - Google Patents
A kind of microorganism mixed bacterial promoting photosynthesis of plant Download PDFInfo
- Publication number
- CN110257298A CN110257298A CN201910600295.9A CN201910600295A CN110257298A CN 110257298 A CN110257298 A CN 110257298A CN 201910600295 A CN201910600295 A CN 201910600295A CN 110257298 A CN110257298 A CN 110257298A
- Authority
- CN
- China
- Prior art keywords
- plant
- mixed bacterial
- microorganism
- cell
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of microorganism mixed bacterials for promoting photosynthesis of plant, belong to agricultural microorganism technical field.It is made of Fuji galactococcus, Azospirillum brasilense, meadow Sphingol single-cell.The mixed bacterial is prepared using following methods: Fuji galactococcus, Azospirillum brasilense, meadow Sphingol single-cell is seeded to respectively in LB culture medium, it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600Then ≈ 2.0 is mixed according to the volume ratio of 1:1:1 to obtain the final product.Microorganism mixed bacterial of the invention, which has, promotes photosynthesis of plant, improves the effect of crop yield.
Description
Technical field
The present invention relates to a kind of microorganism mixed bacterials for promoting photosynthesis of plant, belong to agricultural microorganism technology neck
Domain.
Background technique
Promoting volume increase is the top priority of modern agriculture with high efficiency.In order to realize the purpose, growing industry technical staff is past
Toward fertilizer, especially chemical fertilizer blindly is excessively used, this not only will increase planting cost, waste of resource, will also result in arable soil
The problems such as hardened acidification declines with quality of agricultural product, the face source for also seriously affecting surface water and groundwater quality of accompanying this
Pollution.Therefore, control fertilizer amount is the real needs for promoting cost-saving synergistic, energy-saving and emission-reduction, to guarantee national food security, agriculture
Product quality and safety and Agri-ecological security have a very important significance.And research and development of functional microorganism fertilizer and agricultural prebiotic
The screening compounding of bacterium is to realize one of the important means of Reducing amount of chemical fertilizer applied.
Some functional microorganisms can secrete typical plant hormone --- the basic element of cell division near root system of plant, to promote
Into the photosynthesis of plant.The basic element of cell division (cytokinin, CTK) is a kind of substance for promoting cytokinesis, promotes a variety of groups
The differentiation and growth knitted can delay the degradation of phytoprotein and chlorophyll, delay senility.
The functional microorganism that plant hormone can be secreted near root system of plant is known as growth-promoting rhizosphere bacterium (plant growth
promoting rhizobacteria,PGPR).It is proposed development, the exploitation of the composition and production technology composite bacteria agent of novel PGPR
It is the direction of one hot spot of present microorganism fertilizer with application.Compound purpose is between the bacterial strain made including PGPR, with
And PGPR is combined with other profitable strains, reaches the complementation of growth-promoting effect and the amplification of efficiency.
Compounding microbial inoculum is formed with secretory cell mitogen bacterial strain, for promoting photosynthesis of plant, improves crop yield tool
There is remarkable result, has broad application prospects in agricultural production.
Summary of the invention
The object of the present invention is to provide a kind of mixed bacterials being made of multiple microbial strains, it is by having cell point
Split Fuji galactococcus, the Azospirillum brasilense, meadow Sphingol single-cell composition of plain secretion capacity.The microorganism mixed bacterial
With photosynthesis of plant is promoted, the effect of crop yield is improved.
The invention adopts the following technical scheme:
It is a kind of promote photosynthesis of plant microorganism mixed bacterial, it be by Fuji galactococcus, Azospirillum brasilense,
Meadow Sphingol single-cell composition.The mixed bacterial is prepared using following methods: Fuji galactococcus, Brazil is solid
Nitrogen spirillum, meadow Sphingol single-cell are seeded to respectively in LB culture medium, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈ 2.0, so
It is mixed according to the volume ratio of 1:1:1 afterwards to obtain the final product.
Wherein, Fuji galactococcus Lactococcus fujiensis CGMCC10453, China General Microbiological strain
Preservation administrative center (China General Microbiological Culture Collection Center, CGMCC),
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on May 10th, 2010, deposit number: CGMCC
No.10453。
Wherein, Azospirillum brasilense Azospirillum brasilense CGMCC10379, China General Microbiological bacterium
Kind preservation administrative center (China General Microbiological Culture Collection Center,
CGMCC), address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on 2 2nd, 2010, deposit number: CGMCC
No.10379。
Wherein, meadow Sphingol single-cell Sphingomonas prati CGMCC15645, China General Microbiological strain
Preservation administrative center (China General Microbiological Culture Collection Center, CGMCC),
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on March 5th, 2016, deposit number: CGMCC
No.15645。
Fuji galactococcus Lactococcus fujiensis CGMCC10453, the Azospirillum brasilense that the present invention uses
Azospirillum brasilense CGMCC10379, meadow Sphingol single-cell Sphingomonas prati
CGMCC15645 has basic element of cell division secretion capacity, after special ratios are used in compounding, for promoting photosynthesis of plant, mentions
High crop yield has remarkable result, has broad application prospects in agricultural production.
Outstanding advantage of the invention is:
1. mixed bacterial of the invention is better than single bacterial strain to the facilitation of photosynthesis of plant, and in practical soil root
It is more adaptable in the complex environment of border.
2. the mixed bacterial mix proportion scheme of determining bacterial strain quantity and ratio is better than the mixed bacterial in primal environment, because matching
Stably and controllable, the definite functions in side.
3. bacteria combination is combined better than fungi.The Bacteria Culture time is shorter, and the fungal culture time is longer, therefore, bacterium training
It supports efficiency and is better than fungi.In addition, bacterium is compared with fungi, and it is stronger to the tolerance of complicated adverse circumstances, in practical rhizosphere soil
It is more adaptable in environment.
Specific embodiment
Below with reference to specific example, the present invention will be further described.
Embodiment 1
Microorganism mixed bacterial promotes hydroponic plant growth
Specific implementation step is as follows:
Fuji galactococcus Lactococcus fujiensis CGMCC10453 (being denoted as bacterium A), Azospirillum brasilense
Azospirillum brasilense CGMCC10379 (being denoted as bacterium B), meadow Sphingol single-cell Sphingomonas
Prati CGMCC15645 (is denoted as bacterium C), is seeded in LB culture medium respectively, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈
2.0, it is mixed respectively by the volume ratio of 1:1:1,1:2:1,1:1:2,2:1:1, while choosing two of them bacterium respectively with the body of 1:1
Product is than mixing, then takes the single bacterium culture solution of three kinds of bacterium.Above-mentioned each culture solution is according to 106The microorganism final concentration of CFU/ml is added
Culture 1 week is continued at the rape rhizosphere position of hydroponic culture systems.The present invention also sets up one group of blank control experiment, i.e., in identical experiment
Under the conditions of hydroponic culture systems rape rhizosphere position be added equivalent clear water as blank control group.Detect photosynthetic rate, chlorophyll
The indexs such as content, total dry weight, as a result as shown in table 1 below.
The mixed bacterial of the present invention of table 1 is on the photosynthetic influence experimental result of hydroponic plant
By embodiment 1 it is found that three plants of bacterium all exist and in 1:1:1 ratio in use, either photosynthetic rate, chlorophyll contain
Amount or total dry weight, function and effect are all optimal.
Embodiment 2
Microorganism mixed bacterial promotes earth culture plant growth
Specific implementation step is as follows:
Fuji galactococcus Lactococcus fujiensis CGMCC10453 (being denoted as bacterium A), Azospirillum brasilense
Azospirillum brasilense CGMCC10379 (being denoted as bacterium B), meadow Sphingol single-cell Sphingomonas
Prati CGMCC15645 (is denoted as bacterium C), is seeded in LB culture medium respectively, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈
2.0, it is mixed respectively by the volume ratio of 1:1:1,1:2:1,1:1:2,2:1:1, while choosing two of them bacterium respectively with the body of 1:1
Product is than mixing, then takes the single bacterium culture solution of three kinds of bacterium.Above-mentioned each culture solution is according to 106The microorganism final concentration of CFU/ml is added
Culture 1 week is continued at the rape rhizosphere position of earth culture system.Under same test conditions, add at the rape rhizosphere position of earth culture system
Enter equivalent clear water as blank control group.The indexs such as photosynthetic rate, chlorophyll content, total dry weight are detected, as a result such as the following table 2 institute
Show.
Influence experimental result of the mixed bacterial of the present invention of table 2 to earth culture photosynthesis of plant
By embodiment 2 it is found that three plants of bacterium all exist and it is optimal in 1:1:1 ratio Use Limitation fruit.Earth culture plant is come
It says, compared with blank control, the either increment of photosynthetic rate, chlorophyll content or total dry weight, micro- life of the invention
The effect of object mixed bacterial is better than the sum of the effect of three kinds of single bacterial strains.This is because mixed bacterial of the invention is using specific
The bacterial strain type of ratio mixes, and has the function of synergy in rhizosphere in situ environment between bacterial strain, actual effect is better than
Single bacterial strain, it is more adaptable in practical rhizosphere soil complex environment.Determine the mixed bacterial proportion of bacterial strain quantity and ratio
Scheme is better than the mixed bacterial in primal environment, right after special ratios are used in compounding because formulation stable is controllable and definite functions
It is significant in the facilitation effect of photosynthesis of plant.In addition, there are many microorganisms in mixed microorganism flora of the invention, it is different micro-
Biology can also interact between generating different metabolites, promote the good metabolism of entire flora system, also promote plant
The photosynthesis of object.
Embodiment 3
Microbial strains secretory cell mitogen aptitude tests
Specific implementation step is as follows:
Fuji galactococcus Lactococcus fujiensis CGMCC10453 (being denoted as bacterium A), Azospirillum brasilense
Azospirillum brasilense CGMCC10379 (being denoted as bacterium B), meadow Sphingol single-cell Sphingomonas
Prati CGMCC15645 (is denoted as bacterium C), is seeded in LB culture medium respectively, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈
2.0.Three plants of bacteria culture fluid 100mL are taken respectively, using liquid chromatography-tandem mass spectrometry Cytokinin Contents therein,
Such as the following table 3.
3 strain secretes basic element of cell division experimental result of table
By embodiment 3 it is found that three plants of bacterium all have the ability of secretory cell mitogen, can promote to plant in rhizospheric environment
Object photosynthesis improves crop yield.It can be seen that microorganism mixing of the present invention from the experimental data of embodiment 1 and embodiment 2
Flora is better than the sum of the effect of three kinds of single bacterial strains to the facilitation effect of photosynthesis of plant.This is because having between hybrid bacterial strain
There is synergy, the good metabolism of entire flora system also promotes the photosynthesis of plant.
Claims (4)
1. a kind of microorganism mixed bacterial for promoting photosynthesis of plant, which is characterized in that it is by Fuji galactococcus, Brazil
Azospirillum, meadow Sphingol single-cell composition；The mixed bacterial is prepared using following methods: by Fuji cream
Coccus, Azospirillum brasilense, meadow Sphingol single-cell are seeded to respectively in LB culture medium, and 30 DEG C of shaken cultivations are dense to bacterium to be
O.D600Then ≈ 2.0 is mixed according to the volume ratio of 1:1:1 to obtain the final product.
2. the microorganism mixed bacterial according to claim 1 for promoting photosynthesis of plant, which is characterized in that the Fuji
Mountain galactococcus is Fuji galactococcus CGMCC10453.
3. the microorganism mixed bacterial according to claim 1 for promoting photosynthesis of plant, which is characterized in that described Brazil
Azospirillum is Azospirillum brasilense CGMCC10379.
4. the microorganism mixed bacterial according to claim 1 for promoting photosynthesis of plant, which is characterized in that the meadow
Sphingol single-cell is meadow Sphingol single-cell CGMCC15645.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910600295.9A CN110257298B (en) | 2019-07-04 | 2019-07-04 | Microorganism mixed flora for promoting plant photosynthesis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910600295.9A CN110257298B (en) | 2019-07-04 | 2019-07-04 | Microorganism mixed flora for promoting plant photosynthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110257298A true CN110257298A (en) | 2019-09-20 |
| CN110257298B CN110257298B (en) | 2021-03-26 |
Family
ID=67924454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910600295.9A Active CN110257298B (en) | 2019-07-04 | 2019-07-04 | Microorganism mixed flora for promoting plant photosynthesis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110257298B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11980191B2 (en) | 2022-04-25 | 2024-05-14 | South China Agricultural University | Compound mycorrhizal fungus growth promoting agent and agricultural-photovoltaic complementary planting method for Dioscorea composita |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102153381A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Composite microbial fertilizer and low temperature embedding preparation method thereof |
| CN102965307A (en) * | 2012-11-09 | 2013-03-13 | 广东省土壤科学博物馆 | Azospirillum brasilense strain and microbial preparation thereof |
| CN109022414A (en) * | 2018-09-10 | 2018-12-18 | 中国环境科学研究院 | A kind of bloodstone coupled biological charcoal and the application in removal percolate organic matter |
| CN109081454A (en) * | 2018-09-10 | 2018-12-25 | 中国环境科学研究院 | Iron restores biochemical treatment to the decrement method of landfill leachate membrane process concentrate |
-
2019
- 2019-07-04 CN CN201910600295.9A patent/CN110257298B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102153381A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Composite microbial fertilizer and low temperature embedding preparation method thereof |
| CN102965307A (en) * | 2012-11-09 | 2013-03-13 | 广东省土壤科学博物馆 | Azospirillum brasilense strain and microbial preparation thereof |
| CN109022414A (en) * | 2018-09-10 | 2018-12-18 | 中国环境科学研究院 | A kind of bloodstone coupled biological charcoal and the application in removal percolate organic matter |
| CN109081454A (en) * | 2018-09-10 | 2018-12-25 | 中国环境科学研究院 | Iron restores biochemical treatment to the decrement method of landfill leachate membrane process concentrate |
Non-Patent Citations (2)
| Title |
|---|
| DALIA MOLINA-ROMERO等: "Compatible Bacterial Mixture, Tolerant to Desiccation, Improves Maize Plant Growth", 《PLOS ONE》 * |
| 张琼琼等: "湿地植物内生细菌多样性研究进展", 《湿地科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11980191B2 (en) | 2022-04-25 | 2024-05-14 | South China Agricultural University | Compound mycorrhizal fungus growth promoting agent and agricultural-photovoltaic complementary planting method for Dioscorea composita |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110257298B (en) | 2021-03-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103964946B (en) | A kind of paddy rice Special compound microbial fertilizer and preparation method thereof | |
| US10287216B2 (en) | Method for directly preparing a fertilizer by using straws and prepared straw organic fertilizer | |
| CN110241051A (en) | A kind of microorganism mixed bacterial promoting plant shoot growth | |
| CN110184220B (en) | Efficient phosphate and potassium solubilizing bacterium and application thereof | |
| CN103570434B (en) | A kind of microbial inoculum, liquid microbe fertilizer, its production method and application | |
| CN110272844A (en) | A kind of microorganism mixed bacterial promoting plant root growth | |
| US20170073279A1 (en) | Facility secondary no3- salinized soil modifier, preparation method and modification method | |
| CN102557837A (en) | Halophilic and alkalophilic microorganism solid bacterial fertilizer of saline-alkaline habitat of desert and preparation method and application thereof | |
| CN104003804A (en) | Moisture-retaining and nitrogen-fixing compound microbial fertilizer and preparation method thereof | |
| CN103614326A (en) | High-temperature compost decay-promoting bacterial compound inoculant and applications thereof | |
| CN105294183A (en) | Multifunctional liquid biological fertilizer and production method therefor | |
| CN104498040A (en) | Application of Bacillus amyloliquefaciens as soil conditioner | |
| US20240132420A1 (en) | Joint control method for nitrogen and phosphorus emissions | |
| CN102409014A (en) | Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth | |
| CN105646095A (en) | Special bioorganic fertilizer for leafy vegetables | |
| CN103951486A (en) | Plant nutrition regulation liquid fertilizer and its production method | |
| CN104928203A (en) | Bacillus mucilaginosus and high density fermenting method thereof | |
| CN103194410A (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN104496725B (en) | Organic bacterial manure for lead zinc ore contaminated soil remediation and preparation method of organic bacterial manure | |
| CN106116741A (en) | A kind of Fructus Fragariae Ananssae special organic and inorganic active silicon fertilizer | |
| CN104450573A (en) | Compound ecological microbial agent containing microbial polysaccharide and functional microbes | |
| CN110257298A (en) | A kind of microorganism mixed bacterial promoting photosynthesis of plant | |
| CN102515889A (en) | Production method for biocontrol water flush fertilizer, fertilizer and application | |
| CN102424615A (en) | Special composite microbial fertilizer for tobacco use and its preparation method | |
| CN103772008B (en) | A kind of composite microbiological fertilizer and production method thereof with efficient disease-proof functions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |