CN109081454A - Iron restores biochemical treatment to the decrement method of landfill leachate membrane process concentrate - Google Patents
Iron restores biochemical treatment to the decrement method of landfill leachate membrane process concentrate Download PDFInfo
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- CN109081454A CN109081454A CN201811050588.6A CN201811050588A CN109081454A CN 109081454 A CN109081454 A CN 109081454A CN 201811050588 A CN201811050588 A CN 201811050588A CN 109081454 A CN109081454 A CN 109081454A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
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Abstract
Decrement method of a kind of iron reduction biochemical treatment to landfill leachate membrane process concentrate: under anaerobic environment, using bloodstone particle as microbe carrier and electron acceptor, introduce the decrement method medium that mixing iron-reducing bacterium kind is carried on bloodstone surface, by chemical coordination and biooxidation reactions, hardly degraded organic substance and small organic molecule in landfill leachate are removed.The present invention can effectively cut down the yield of landfill leachate tradition membrane process film concentrate, while reduce membrane process cost.
Description
Technical field
The present invention relates to the improved methods of landfill leachate membrane process, relate more specifically to iron reduction biochemical treatment to rubbish
The decrement method of percolation liquid membrane technique concentrate.
Background technique
The percolate generated in landfill yard is a kind of high-concentration waste water with acute and chronic toxicity.In " house refuse
Landfill percolate handles engineering legislation (HJ-564-2010) " in, percolate treating process can be divided into pretreatment, biology
It handles and three kinds of advanced treating.The percolate processing of China's refuse landfill first passes through flocculation precipitation pretreatment mostly, is passing through
O/A-MBR group technology removes the pollutant in landfill leachate, and COD concentration can control in 400-500mg/L, ammonia nitrogen after processing
Removal rate be 80%-90%, then major part treatment process in can use reverse osmosis membrane processing percolate, treated diafiltration
Liquid can be with qualified discharge.
Landfill leachate membrane process concentrate (hereinafter referred to as film concentrate) is landfill leachate after biodegrade
The residual solution retained by nanofiltration (or reverse osmosis membrane) does not have biodegradability generally, and main component is humus material, in palm fibre
Black, organic concentration is very high, and contains a large amount of metal ion, mishandling to easily cause serious secondary pollution.
The volume of film concentrate accounts for the 8-20% of landfill leachate stoste volume, and transfer freight is high.At conventional film concentrate
Reason method such as coagulating sedimentation, evaporation and concentration or absorption are burned etc. or treatment effect is bad or use condition is harsh, processing cost
It is higher, it is therefore necessary to find the decrement method of new membrane concentrate.
Some iron also pathogenic microorganism can be using Fe (III) as electron acceptor, with the reduction-states substance such as organic matter or hydrogen
As electron donor, vital metabolic activity is carried out.Some researches show that humus materials can be used as electron shuttle body, promotes three
The biological reducing process of valence iron oxide, and then accelerate the oxidation of reduction-state substance.It is mainly shown as: trivalent iron oxide
Humus material can be adsorbed, in the case where iron restores microbial action, the two forms ligand, accelerates organic matter anaerobism raw
The process of object oxidation.Bloodstone (α-Fe2O3) one kind as trivalent iron oxide, widely distributed in nature, reserves
It is higher.A large amount of research has proven to biochemical treatment ability of the bloodstone as electron acceptor to reduction-state pollutant at present.Red iron
Mine is that the iron of electron acceptor restores biochemical treatment process, and the reduction and processing for film concentrate have certain potentiality.
Summary of the invention
The purpose of the present invention is to provide a kind of reduction biochemical treatments of iron to the decrement method of film concentrate, also to mixing iron
The anaerobic biological oxidation organic matter process of opportunistic pathogen driving is strengthened, and provides a kind of economy for the decrement of conventional film concentration liquid measure and has
The method of effect.
To achieve the above object, iron reduction biochemical treatment provided by the invention subtracts landfill leachate membrane process concentrate
Amount method is:
Under anaerobic environment, using bloodstone particle as microbe carrier and electron acceptor, it is negative to introduce mixing iron-reducing bacterium kind
It is loaded in the decrement method medium on bloodstone surface, by chemical coordination and biooxidation reactions, removes difficult drop in landfill leachate
Solve organic matter and small organic molecule.
The decrement method, wherein for bloodstone by crushing and screening, partial size used is 2-4mm.
The decrement method, wherein mixing iron-reducing bacterium kind include Sphingomonas paucimobilis,
Genera Lactococcus、Genera Azospirillum、Geobacillus stearothermophilus、
Pseudomonas spp., Aquabacterium strains and Hydrogenophaga, by concentration ratio 5-7:10-12:4-6:
4-6:6-8:9-11:10-12 is mixed, the mixed bacteria of preparation Anaerobic culturel in iron-reducing bacterium fluid nutrient medium.
The decrement method, wherein iron-reducing bacterium Liquid Culture based formulas are as follows: NH4Cl 0.535g/L, KH2PO4
0.136g/L, MgCl2·6H2O 0.204g/L, CaCl2·2H2O 0.147g/L, NaHCO32.52g/L CH3COONa
0.82g/L, ironic citrate 0.735g/L, microelement mixed solution 10mL/L, vitamin mixed solution 10mL/L.
The decrement method, wherein mixing iron-reducing bacterium kind is carried on bloodstone surface and is divided into inoculation membrane formation method and oneself
Right biofilm method.
The decrement method, wherein inoculation membrane formation method is that bloodstone is soaked in the culture medium for including mixed bacteria
And it is continually fed into nitrogen, it is separated by solid-liquid separation, decrement method medium of the gained solid phase as concentrate.
The decrement method, wherein natural membrane method is that bloodstone is soaked in the culture medium for including mixed bacteria
It directly impregnates, then directly using solid, liquid mixed system as the decrement method medium of concentrate.
The invention has the advantages that and the utility model has the advantages that
(1) bloodstone and mixed bacteria (Sphingomonas paucimobilis, Genera used in the present invention
Lactococcus、Genera Azospirillum、Geobacillus stearothermophilus、Pseudomonas
Spp., Aquabacterium strains and Hydrogenophaga) it is widely distributed, cheap, the practicability of method is high；
(2) operating process of the present invention is easy, and additional energy source and manual maintenance are not necessarily in application process, is suitable for rubbish
The removal of hardly degraded organic substance and small organic molecule in percolate.
(3) method for treating garbage percolation liquid of the present invention takes full advantage of most ancient life process-anaerobic organism oxygen
The principle of change cuts down the yield of film concentrate, solves the problems, such as that the processing of rubbish percolation liquid membrane concentrate is difficult to a certain extent.
Detailed description of the invention
Fig. 1 is the principle that iron restores that rubbish percolation liquid membrane concentration output of fluid is cut down in biochemical treatment；
Fig. 2 is the process flow that iron restores that rubbish percolation liquid membrane concentration output of fluid is cut down in biochemical treatment；
Fig. 3 is the effect that iron restores that rubbish percolation liquid membrane concentration output of fluid is cut down in biochemical treatment, and wherein Fig. 3 a is that COD is dense
Degree, Fig. 3 b is DOC concentration, in figure: ■ is abiogenic reaction system, ▲ for biological reaction system, △ is Napier natural logrithm；
Fig. 4 is the organic component identification that iron restores that rubbish percolation liquid membrane concentration output of fluid is cut down in biochemical treatment, wherein Fig. 4 a
For abiogenic reaction system, Fig. 4 b is biological reaction system；In figure: 1 be to sylvan (9.62min), 2 is isononanoic acid
(10.23min), 3 be caprylic acid (12.23min), 4 be monomethyl succinate (13.68min), 5 be pentamethyl indoles
(15.99min), 6 be 3- butyne-2-alcohol (16.02), 7 be 3,5- di-tert-butyl alcohol (18.63min), 8 be myristyl two
Methyl tertiary amine (19.55min), 9 be hexadecylic acid (20.67min), 10 be diisobutyl phthalate (22.24min), 11 be
Palmitamide (23.74min), 12 be oleamide (25.19min), 13 be two 2- ethylhexyl of phthalic acid) ester
(26.22min)。
Specific embodiment
Present invention employs following technical solutions, in which:
For bloodstone by crushing and screening, partial size used is 2-4mm；
Under anaerobic environment, with bloodstone particle (partial size 2-4mm) for microbe carrier and electron acceptor, mixing iron is introduced
Reduction strain is carried on bloodstone surface in a manner of impregnating biofilm, by chemical coordination, biooxidation reactions, to garbage filter
Liquid (initial COD concentration 500-700mgL-1) in hardly degraded organic substance (such as the humus macromolecular such as humic acid, fulvic acid class)
And small organic molecule (such as volatile fatty acid, halogenated hydrocarbons etc.) removal, removal rate is up to 80% or more；Part has
Machine object includes: to sylvan, isononanoic acid, caprylic acid, monomethyl succinate, pentamethyl indoles, 3- butyne-2-alcohol, bis- uncle of 3,5-
Butyl benzene alcohol, dodecyldimethylamine base tertiary amine, hexadecylic acid, diisobutyl phthalate, palmitamide, oleamide, adjacent benzene two
Two 2- ethylhexyl of formic acid；
Mixing iron-reducing bacterium kind includes Sphingomonas paucimobilis, Genera Lactococcus, Genera
Azospirillum、Geobacillus stearothermophilus、Pseudomonas spp.、Aquabacterium
The strains such as strains and Hydrogenophaga are mixed, the mixed bacteria of preparation by concentration ratio 6:11:5:5:7:10:11
Anaerobic culturel 10~30 days in iron-reducing bacterium fluid nutrient medium；
The iron-reducing bacterium Liquid Culture based formulas are as follows: NH4Cl (0.535g/L), KH2PO4(0.136g/L), MgCl2·
6H2O (0.204g/L), CaCl2·2H2O (0.147g/L), NaHCO3(2.52g/L), CH3COONa (0.82g/L), ironic citrate
(0.735g/L), microelement mixed solution (10mL/L), vitamin mixed solution (10mL/L)；
The mixing iron-reducing bacterium kind is stored in 30% glycerine water solution to be utilized in the freezen protective at -80 DEG C, use
Fluid nutrient medium activates the mixed bacteria saved；
Microorganism, which impregnates biofilm mode, to be divided into: inoculation membrane formation method and natural membrane method.Inoculation membrane formation method is divided into following step
It is rapid: bloodstone being soaked in the culture medium for including mixed bacteria and is continually fed into nitrogen 12~24 hours；It is separated by solid-liquid separation, gained
Decrement method medium of the solid phase as concentrate.Natural membrane method is that bloodstone is soaked in the culture medium for including mixed bacteria
It directly impregnates 12-24 hours, then directly using solid, liquid mixed system as the decrement method medium of concentrate.
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this hair
It is bright to be described in further detail.
Decrement method working principle of the iron reduction biochemical treatment of the present invention to film concentrate are as follows:
As shown in Figure 1, the concentrate decrement method is under anaerobic environment, bloodstone particle (partial size 2-4mm) is served as
Microbe carrier and electron acceptor, introducing iron reduction mixed bacterial (Sphingomonas paucimobilis,
Lactococcus、Azospirillum、Geobacillus stearothermophilus、Pseudomonas spp.、
Aquabacterium strains and Hydrogenophaga), Humus organic matter (HA) plays chemical coordination in percolate
Organic matter biooxidation reactions are completed in effect, and to landfill leachate, (initial COD is 500-700mgL-1, BOD/COD < 0.3)
Middle hardly degraded organic substance (such as the macromoleculars such as humic acid, fulvic acid Humus) and small organic molecule (such as volatility
Fatty acid, halogenated hydrocarbons, benzene homologues etc.) removal, mineralization product is bicarbonate, carbon dioxide.The present invention can effectively cut down rubbish
The yield of percolate tradition membrane process film concentrate.
More specifically, by bloodstone by crushing and screening partial size as 2-4mm.It sieves obtained bloodstone and passes through inoculation membrane formation
It is mixed with the mode of natural membrane with iron-reducing bacterium kind is mixed, carries out impregnating load biofilm.The mixed bacteria
Sphingomonas paucimobilis、Genera Lactococcus、Genera Azospirillum、Geobacillus
Stearothermophilus, Pseudomonas spp., Aquabacterium strains and Hydrogenophaga.It presses
Strain concentration ratio 6:11:5:5:7:10:11 is mixed, the mixed bacteria of preparation Anaerobic culturel in iron reduction fluid nutrient medium
10-30 days.
The iron restores liquid culture medium prescription are as follows: NH4Cl (0.535g/L), KH2PO4(0.136g/L), MgCl2·6H2O
(0.204g/L), CaCl2·2H2O (0.147g/L), NaHCO3(2.52g/L), CH3COONa (0.82g/L), ironic citrate
(0.735g/L), microelement mixed solution (10mL/L), vitamin mixed solution (10mL/L).
The mixed bacteria, which is stored in 30% glycerine water solution, to be restored in the freezen protective at -80 DEG C, use using iron
Fluid nutrient medium activates the mixed bacteria saved.
The inoculation membrane formation method is divided into following steps: bloodstone is soaked in the culture medium for including mixed bacteria and is continued
It is passed through nitrogen 12-24 hours；It is separated by solid-liquid separation, decrement method solid-phase media of the gained solid phase as concentrate.
The iron reduction process is as shown in Figure 2 to film concentrate processing flow schematic diagram.The bloodstone for hanging film can be used for
To hardly degraded organic substance absorption and small organic molecule oxidation removal in garbage leachate treatment process, treated, and percolate can
Film process is carried out again.
Fig. 3 is the effect that iron restores that rubbish percolation liquid membrane concentration output of fluid is cut down in biochemical treatment, it is known that, initial COD concentration
500-700mg·L-1When, hardly degraded organic substance (such as the humus macromolecular such as humic acid, fulvic acid class) and small in percolate
Molecular organic (such as volatile fatty acid, halogenated hydrocarbons etc.) can be efficiently removed, and removal rate is up to 80% or more.Fig. 4 is
Iron restores the organic component identification that rubbish percolation liquid membrane concentration output of fluid is cut down in biochemical treatment, and partial organic substances include: to methyl
Phenol, isononanoic acid, caprylic acid, monomethyl succinate, pentamethyl indoles, 3- butyne-2-alcohol, 3,5- di-tert-butyl alcohol, the tetradecane
Base dimethyl tertiary amine, hexadecylic acid, diisobutyl phthalate, palmitamide, oleamide, two 2- ethylhexyl of phthalic acid
Ester.
Particular embodiments described above has carried out further in detail the purpose of the present invention, technical scheme and beneficial effects
Describe in detail bright, it should be understood that the above is only a specific embodiment of the present invention, is not intended to restrict the invention, it is all
Within the spirit and principles in the present invention, any modification, equivalent substitution, improvement and etc. done should be included in protection of the invention
Within the scope of.
Claims (7)
1. a kind of iron reduction biochemical treatment is to the decrement method of landfill leachate membrane process concentrate:
Under anaerobic environment, using bloodstone particle as microbe carrier and electron acceptor, introduces mixing iron-reducing bacterium kind and be carried on
The decrement method medium on bloodstone surface, it is difficult to degrade in removal landfill leachate to have by chemical coordination and biooxidation reactions
Machine object and small organic molecule.
2. decrement method according to claim 1, wherein for bloodstone by crushing and screening, partial size used is 2-4mm.
3. decrement method according to claim 1, wherein mixing iron-reducing bacterium kind includes Sphingomonas
paucimobilis、Genera Lactococcus、Genera Azospirillum、Geobacillus
Stearothermophilus, Pseudomonas spp., Aquabacterium strains and Hydrogenophaga, are pressed
Concentration ratio 5-7:10-12:4-6:4-6:6-8:9-11:10-12 is mixed, and the mixed bacteria of preparation is trained in iron-reducing bacterium liquid
Support Anaerobic culturel in base.
4. decrement method according to claim 3, wherein iron-reducing bacterium Liquid Culture based formulas are as follows: NH4Cl 0.535g/
L, KH2PO40.136g/L, MgCl2·6H2O 0.204g/L, CaCl2·2H2O 0.147g/L, NaHCO32.52g/L
CH3COONa 0.82g/L, ironic citrate 0.735g/L, microelement mixed solution 10mL/L, vitamin mixed solution 10mL/
L。
5. decrement method according to claim 1, wherein mixing iron-reducing bacterium kind is carried on bloodstone surface and is divided into inoculation
Biofilm method and natural membrane method.
6. decrement method according to claim 5, wherein inoculation membrane formation method is to be soaked in bloodstone to include mixed bacteria
Culture medium in and be continually fed into nitrogen, be separated by solid-liquid separation, decrement method medium of the gained solid phase as concentrate.
7. decrement method according to claim 5, wherein natural membrane method is to be soaked in bloodstone to include mixed bacteria
Culture medium in directly impregnate, then directly using solid, liquid mixed system as the decrement method medium of concentrate.
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Cited By (4)
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| CN109775862A (en) * | 2019-01-30 | 2019-05-21 | 中国环境科学研究院 | A kind of permeable reaction wall and underground water pollution biology in situ renovation method |
| CN110257298A (en) * | 2019-07-04 | 2019-09-20 | 临沂大学 | A kind of microorganism mixed bacterial promoting photosynthesis of plant |
| CN112919625A (en) * | 2019-12-06 | 2021-06-08 | 南京理工大学 | Method for promoting degradation of ammonia nitrogen and organic matters in underground water by using starch modified hematite |
| CN115368906A (en) * | 2022-07-01 | 2022-11-22 | 华南理工大学 | Goethite immobilized iron-sulfur reducing bacteria composite material and preparation method and application thereof |
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Application publication date: 20181225 |