CN103725737B - A kind of bioanalysis is processed the method for excess sludge product sludge protein - Google Patents

A kind of bioanalysis is processed the method for excess sludge product sludge protein Download PDF

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Publication number
CN103725737B
CN103725737B CN201310755713.4A CN201310755713A CN103725737B CN 103725737 B CN103725737 B CN 103725737B CN 201310755713 A CN201310755713 A CN 201310755713A CN 103725737 B CN103725737 B CN 103725737B
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China
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bacillus
liquid
sludge
excess sludge
mixed bacteria
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CN201310755713.4A
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Chinese (zh)
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CN103725737A (en
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乔长晟
张宇
彭巧
王羿超
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天津北洋百川生物技术有限公司
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Abstract

The invention discloses a kind of bioanalysis and process the method for excess sludge product sludge protein, belong to biological technical field. It is characterized in that, to adding mass ratio in excess sludge be: the mixed bacteria of bacillus subtilis: bacillus amyloliquefaciens: bacillus licheniformis: bacillus megaterium=5-10:1-5:2-7:1-5, in liquid fermentation fermentor, temperature 35-55 DEG C, throughput is 0.2-0.6L × min-1, heat-preservation fermentation 18-24 hour; Plate compression, filtrate is concentrated, can be as the nutritional agents of fire foam and microorganism; Filtration cakes torrefaction can be used as fertilizer and feed stripped. Both the secondary pollution of having avoided excess sludge to cause, has improved again the value of mud greatly.

Description

A kind of bioanalysis is processed the method for excess sludge product sludge protein

Technical field:

The invention belongs to biological technical field, be specifically related to utilize biological mixed bacteria liquid method to process the side of excess sludge product sludge proteinMethod.

Background technology:

Treatment of Sludge generally adopts activated sludge process now, and Sewage Treatment for Activated Sludge can produce a large amount of excess sludges, surplusRemaining mud contains a large amount of organic matters, disposes improperly, can cause the secondary pollution of environment and the waste of resource. Residue is dirty at presentThe main processing method of mud has: landfill, throwing sea, burning, chemical treatment, physical method processing, biological minimizing processing, onThere is certain deficiency in processing or the recycling of the method for stating to excess sludge. For example conventional physics or chemical method processing, byIn the rising of temperature, can produce a large amount of decomposition of mud, the oxidation of organic substance, adds that proteolysis is the amino of small fragmentAcid, can produce stench, and the advantage of biological minimizing processing is, utilizes the ammonia nitrogen enzyme of bacterial strain generation, can eliminate foul gas.

In excess sludge, contain a large amount of organic matters, wherein protein content is the highest, in 30%-45% left and right, if do not done properlyProcessing, be discharged into the waste that can cause secondary pollution and resource in environment. Therefore utilize biological decrement method to process excess sludge moreMore be subject to the attention of the industry.

Application number: 2006100190637 the Chinese patent " production with quick lime hydrolysis residual actived sludge for aminosalProcesses and apparatus " and number of patent application: " sewage disposal produces processing and the extraction of activated sludge to 021162271 Chinese patentMethod of protein " treatment process of excess sludge is provided, but there is certain shortcoming, and complex process, energy consumption is high, processesAfter sludge protein frowziness, be unfavorable for applying of sludge protein.

Summary of the invention:

The present invention utilizes biological mixed bacteria liquid to process excess sludge by the method for biotechnology and produces sludge protein, with biological sideMethod lignin-sludge, easy, cost is low, processes the sludge protein free from extraneous odour producing, and has realized and having turned waste into wealth, and application prospect is extensive,There is great economy, society and environmental benefit.

The invention provides a kind of bioanalysis and process the method for excess sludge product sludge protein, comprise the steps:

1. in excess sludge, add the mixed bacteria liquid that mass ratio is 1-5%;

Described mixed bacteria liquid is by bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus megaterium composition;In described mixed bacteria liquid, each strain quality portion rate is: bacillus subtilis: bacillus amyloliquefaciens: bacillus licheniformis: hugeBacterium anthracoides=5-10:1-5:2-7:1-5;

Described bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, the cell concentration of bacillus megaterium all exists 3×108Individual/ml to 7 × 108Individual/ml.

2. the excess sludge that is added with mixed bacteria liquid is added in liquid fermentation fermentor and fermented, temperature 35-55 DEG C, throughput is0.2-0.6L×min-1, heat-preservation fermentation 18-24 hour;

3. plate compression, concentrated.

Excess sludge after treatment, through plate compression, liquid part, through concentrated, can be used as fire foam and microorganismNutritional agents; Filter cake after plate compression, the solid portion of excess sludge can be used as fertilizer and feed stripped after being dried.

Beneficial effect

The invention provides a kind of novel biological mixed bacteria liquid and process the method for excess sludge product sludge protein, utilize bacillus subtilisBacterium and bacillus amyloliquefaciens decompose excess sludge, and bacillus amyloliquefaciens can decompose carbohydrate, promote bacillus subtilisProduce ectoenzyme, decompose mud; Bacillus megaterium can be decomposed the Phosphorus material in excess sludge, prevents that mud from polluting again;Bacillus licheniformis can be processed the water body after excess sludge, suppresses the growth of miscellaneous bacteria, prevents that stench from producing. Avoid excess sludgeThe secondary pollution causing, produced simultaneously sludge protein, pollution-free, unharmful substance is residual, can be as fire foam andMicrobial nutrition agent, the solid portion of excess sludge contains the undecomposed albumen of part and organic matter, can be as organic after being driedFertilizer and feed stripped, improved the value of mud greatly.

Detailed description of the invention:

Below by specific embodiment narration the present invention. Unless stated otherwise, in the present invention, technological means used is thisThe method that those skilled in the art are known. In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention,The spirit and scope of the invention are only limited by claims. To those skilled in the art, do not deviating from the present invention in factUnder the prerequisite of matter and scope, various changes that the material component in these embodiments and consumption are carried out or change and also belong to thisBright protection domain.

Embodiment 1

1. add the excess sludge of moisture content 98% to the fermentation tank of 5L, then add biological mixed bacteria liquid, biological bacteria liquid total addition level is dirty4.5% of shale amount, wherein bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, the adding of bacillus megaterium liquidDosage is respectively 5 parts, 1 part, 2 parts, 1 part; , wherein bacillus subtilis liquid and the concrete concentration of bacillus amyloliquefaciens liquid exist5.5×108-6.0×108Individual/ml, bacillus megaterium and Bacillus licheniformis bulk concentration are 5.0 × 108-5.5×108Individual/ml。

2. the excess sludge that has added biological mixed bacteria liquid is added to liquid fermentation fermentor, 45 DEG C, throughput is 0.4L × min-1,Heat-preservation fermentation 20 hours, finally by plate compression, filtrate is concentrated, obtains sludge protein liquid, can be as fire foam andMicrobial nutrition agent. Filter cake after plate compression, can be used as fertilizer and feed stripped after being dried.

3. biological bacteria liquid adopts first order seed to cultivate and secondary seed cultivation

First order seed is cultivated: the solid-state slant activation bacillus subtilis of broth bouillon, bacillus amyloliquefaciens, lichens gemma barBacterium, bacillus megaterium is cultivated 12h in 45 DEG C of constant incubators.

Secondary seed is cultivated: by bacillus subtilis, and bacillus amyloliquefaciens, bacillus licheniformis, bacillus megaterium inoculationTo broth bouillon 500ml triangular flask, liquid amount 70ml, at 45 DEG C, 200rpm, shaking table is cultivated 12h, through coating nutritionAgar plate, calculates the concrete concentration of each bacterium 3 × 108-6×108Individual/ml.

Embodiment 2

1. add the excess sludge of moisture content 98% to the fermentation tank of 5L, then to add biological mixed bacteria liquid biological bacteria liquid total addition level be mudQuality 5%, wherein bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, the addition of bacillus megaterium liquid dividesIt is not 7 parts, 3 parts, 4 parts, 3 parts; , wherein bacillus subtilis liquid and the concrete concentration of bacillus amyloliquefaciens liquid are 5.5 × 108-6.0×108Individual/ml, bacillus megaterium and Bacillus licheniformis bulk concentration are 5.0 × 108-5.5×108Individual/ml.

2. the excess sludge that has added biological mixed bacteria liquid is added to liquid fermentation fermentor, 55 DEG C, throughput is 0.4L × min-1,Heat-preservation fermentation 18 hours, finally by plate compression, by concentrated, obtains sludge protein liquid, can be as fire foam andMicrobial nutrition agent. Filter cake after plate compression, can be used as fertilizer and feed stripped after being dried.

3. the cultural method of biological bacteria liquid is substantially with embodiment 1.

Embodiment 3

1. add the excess sludge of moisture content 98% to the fermentation tank of 5L, then to add biological mixed bacteria liquid biological bacteria liquid total addition level be mudQuality 4%, wherein bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, the addition of bacillus megaterium liquid dividesIt is not 10 parts, 5 parts, 7 parts, 5 parts; , wherein bacillus subtilis liquid and the concrete concentration of bacillus amyloliquefaciens liquid 5.5 ×108-6.0×108Individual/ml, bacillus megaterium and Bacillus licheniformis bulk concentration are 5.0 × 108-5.5×108Individual/ml.

2. the excess sludge that has added biological mixed bacteria liquid is added to liquid fermentation fermentor, 35 DEG C, throughput is 0.4L × min-1,Heat-preservation fermentation 24 hours, finally by plate compression, by concentrated, obtains sludge protein liquid, can be as fire foam andMicrobial nutrition agent. Filter cake after plate compression, can be used as fertilizer and feed stripped after being dried.

3. the cultural method of biological bacteria liquid is substantially with embodiment 1.

Claims (6)

1. bioanalysis is processed a method for excess sludge product sludge protein, comprises the steps:
1. in excess sludge, add the mixed bacteria liquid that mass ratio is 1-5%;
Described mixed bacteria liquid is by bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus megaterium composition;In described mixed bacteria liquid, each strain quality ratio is: bacillus subtilis: bacillus amyloliquefaciens: bacillus licheniformis: huge budSpore bacillus=5-10:1-5:2-7:1-5;
2. the excess sludge that is added with mixed bacteria liquid is added in liquid fermentation fermentor and fermented, temperature 35-55 DEG C, throughput is 0.2-0.6L×min-1, heat-preservation fermentation 18-24 hour;
3. plate compression, filtrate is concentrated, filtration cakes torrefaction;
Mixed bacteria liquid adopts first order seed to cultivate and secondary seed is cultivated;
First order seed is cultivated: the solid-state slant activation bacillus subtilis of broth bouillon, bacillus amyloliquefaciens, lichens gemma barBacterium, bacillus megaterium is cultivated 12h in 45 DEG C of constant incubators;
Secondary seed is cultivated: by bacillus subtilis, and bacillus amyloliquefaciens, bacillus licheniformis, bacillus megaterium inoculationTo broth bouillon 500ml triangular flask, liquid amount 70ml, at 45 DEG C, 200rpm, shaking table is cultivated 12h.
2. the method that bioanalysis processing excess sludge produces sludge protein according to claim 1, is characterized in that, bacillus subtilis,Bacillus amyloliquefaciens, bacillus licheniformis, the cell concentration of bacillus megaterium is all 3 × 108Individual/ml to 7 × 108Individual/ml.
3. the method that bioanalysis processing excess sludge produces sludge protein according to claim 1, comprises the steps:
1. add the excess sludge of moisture content 98% to fermentation tank, then add mixed bacteria liquid, mixed bacteria liquid total addition level is sludge quality4.5%, described bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, the addition of bacillus megaterium liquid dividesBe not 1.5%, 1.0%, 1.0%, 1.0%, described bacillus subtilis liquid and bacillus amyloliquefaciens liquid concentration are 5.5 × 108-6.0×108Individual/ml, bacillus megaterium and Bacillus licheniformis bulk concentration are 5.0 × 108-5.5×108Individual/ml;
2. the excess sludge that has added mixed bacteria liquid is added to liquid fermentation fermentor, 45 DEG C, throughput is 0.4L × min-1, insulation is sent outFerment 20 hours, by plate compression, filtrate is concentrated, obtains sludge protein liquid.
4. the method that bioanalysis processing excess sludge produces sludge protein according to claim 1, comprises the steps:
1. add the excess sludge of moisture content 98% to fermentation tank, then add mixed bacteria liquid, biological bacteria liquid total addition level is sludge quality5%, described bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, the addition of bacillus megaterium liquid respectivelyBe 1.5%, 1.0%, 1.5%, 1.0%, described bacillus subtilis liquid and bacillus amyloliquefaciens liquid concentration are 5.5 × 108-6.0×108Individual/ml, bacillus megaterium and Bacillus licheniformis bulk concentration are 5.0 × 108-5.5×108Individual/ml;
2. the excess sludge that has added mixed bacteria liquid is added to liquid fermentation fermentor, 55 DEG C, throughput is 0.4L × min-1, insulation is sent outFerment 18 hours, by plate compression, filtrate is concentrated, obtains sludge protein liquid.
5. the method that bioanalysis processing excess sludge produces sludge protein according to claim 1, comprises the steps:
1. add the excess sludge of moisture content 98% to fermentation tank, then add mixed bacteria liquid, mixed bacteria liquid total addition level is sludge quality4%, described bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, the addition of bacillus megaterium liquid respectivelyBe 1.0%, 1.0%, 1.0%, 1.0%, described bacillus subtilis liquid and bacillus amyloliquefaciens liquid concentration are 5.5 × 108-6.0×108Individual/ml, bacillus megaterium and Bacillus licheniformis bulk concentration are 5.0 × 108-5.5×108Individual/ml;
2. the excess sludge that has added mixed bacteria liquid is added to liquid fermentation fermentor, 35 DEG C, throughput is 0.4L × min-1, insulation is sent outFerment 24 hours, by plate compression, filtrate is concentrated, obtains sludge protein liquid.
6. the application of the sludge protein of preparing according to the arbitrary described method of claim 1-5, excess sludge after treatment, presses through sheet frameFilter, after liquid partial concentration, can be as the nutritional agents of fire foam and microorganism; After filtration cakes torrefaction, can be used as fertilizerAnd feed stripped.
CN201310755713.4A 2013-12-27 2013-12-27 A kind of bioanalysis is processed the method for excess sludge product sludge protein CN103725737B (en)

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CN104805032A (en) * 2014-12-29 2015-07-29 天津北洋百川生物技术有限公司 Multifunctional microbial agent for sludge reduction and preparation thereof
CN104529583A (en) * 2015-01-16 2015-04-22 南开大学 Method for producing organic fertilizer by treating sludge by use of microbial technology
CN104789504B (en) * 2015-04-17 2018-06-01 浙江省环境保护科学设计研究院 A kind of microbial bacterial agent being reduced for sludge in-situ and its preparation method and application
CN105385648A (en) * 2015-12-09 2016-03-09 天津北洋百川生物技术有限公司 Screening, preparation, and application method of microbial composite inoculant for reducing residual sludge
CN106987536A (en) * 2017-03-01 2017-07-28 沃邦环保有限公司 Complex micro organism fungicide for river regulation and its preparation method and application
CN106987535A (en) * 2017-03-01 2017-07-28 沃邦环保有限公司 It is a kind of for complex micro organism fungicide of river regulation and preparation method thereof, river sewage processing method

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CN103058476A (en) * 2012-12-31 2013-04-24 天津北洋百川生物技术有限公司 Novel treatment method for excess sludge

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CN1456372A (en) * 2003-04-18 2003-11-19 湖北大学 Preparing for protein foam fire-extinguisher by active sludge protein
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