CN104805032A - Multifunctional microbial agent for sludge reduction and preparation thereof - Google Patents
Multifunctional microbial agent for sludge reduction and preparation thereof Download PDFInfo
- Publication number
- CN104805032A CN104805032A CN201410835555.8A CN201410835555A CN104805032A CN 104805032 A CN104805032 A CN 104805032A CN 201410835555 A CN201410835555 A CN 201410835555A CN 104805032 A CN104805032 A CN 104805032A
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- China
- Prior art keywords
- bacillus
- microbiobacterial agent
- mud
- decrement
- fermented liquid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
Abstract
The invention provides a multifunctional microbial agent for sludge reduction and a preparation method thereof and belongs to the technical field of environmental protection. The microbial agent is composed of bacillus cereus, bacillus amyloliquefaciens, bacillus megatherium and bacillus subtilis fermentation broths according to the volume ratio of 3: 7: 5:8; and then the mixed fermentation broths are blended with a protective agent, and the mixture undergoes freeze drying to prepare a bacterial powder. After the microbial agent for sludge reduction is used to process sludge, sludge reduction amount reaches 28%, and water content is minimized by 30%.
Description
Technical field:
The invention belongs to environmental technology field, relate to a kind of degraded excess sludge multifunctional microbial composite fungus agent and preparation method.
Background technology:
Activated sludge process is as the dirty water living creature processing technique be most widely used in the world, possess skills the features such as maturation, technology category is many, wastewater treatment efficiency is good, but activated sludge process also brings a large amount of by product while disposing of sewage---residual active sludge, sludge quantity usually accounts for 0.3% ~ 0.5% (volume) of sewage quantity or is about 1% ~ 2% (quality) of sewage load; If belong to advanced treatment, sludge quantity also can increase by 0.5 ~ 1 times, and the raising of sewage load and emission standard must cause the increase of amount of sludge.
The aggregate that the organism of the zoogloea that mud is formed by multiple-microorganism and absorption thereof and inorganics form, except containing (can up to more than 80%) except a large amount of moisture, an organism also containing difficult degradation! Heavy metal and salt, and pathogenic micro-organism and parasitic ovum etc. a large amount of undressed mud is stacked arbitrarily and is discharged and causes new pollution to environment, particularly in the higher city of Chinese Urbanization level and area, specific resistance to filtration has been very distinct issues.
End in by the end of June, 2010, build together and establish urban wastewater treatment firm 2389, whole nation sewage treatment capacity reaches 1.15 billion cubic meters/day, the wet mud of 5 ton 80% can be produced according to 10,000 tons of water, China's mud day output can reach 5.75 ten thousand tons, the mud output that wets in year (calculating by 365 days) 2098.75 ten thousand tons.Calculate according to average operating load 80%, the mud output that wets in year (calculating by 365 nature's mysteries) 1,679 ten thousand tons.335.8 ten thousand tons, over dry mud.
The electric charge on biological sludge surface mainly comes from the charged functional groups entrained by extracellular polymeric (EPS) being attached to cell surface.And EPS is bacterium and other microorganisms for self-protection and the natural organic matter that mutually sticks, it is the 3rd large class component of biological sludge except cell and moisture.The main component of EPS is protein and polysaccharide, and both account for 70% ~ 80% of EPS total amount.The reason of active sludge difficulty dehydration is just the existence of EPS.
Summary of the invention:
In order to solve the problem; the invention provides a kind of can the microbiobacterial agent of decrement mud; described microbial inoculum by Bacillus cereus, bacillus amyloliquefaciens, bacillus megaterium and subtilis fermented liquid by volume 3:7:5:8 form, and be equipped with protective material and make bacterium powder through lyophilize.
Described bacillus cereus deposit number is CGMCC1.8641;
Described bacillus amyloliquefaciens deposit number is CGMCC1.1226;
Described bacillus megaterium deposit number is CGMCC1.234;
Described subtilis deposit number is CGMCC1.1630.
Described microbiobacterial agent addition is 0.03% ~ 0.05% (m:v) of mud.
Described protective material is composed as follows: skim-milk, sucrose, L-sodium, vitamins C.
The preparation method of described microbiobacterial agent is as follows:
(1) preparation of fermented liquid
Bacillus cereus, bacillus amyloliquefaciens, bacillus megaterium and subtilis are cultivated respectively as follows:
Seed culture: by inclined plane inoculating in seed culture medium, in 35 ~ 37 DEG C, rotating speed 200 ~ 220rpm cultivates 1 ~ 2 day,
The composition of seed culture medium: extractum carnis 0.5%, sodium-chlor 0.5%, extractum carnis 1%, insufficient section is with distilled water polishing, and pH regulator scope is 7.2 ~ 7.4;
Fermentation culture: nutrient media components is identical with seed culture medium, accesses fermention medium by seed liquor by inoculum size 1 ~ 2%, in 35 ~ 37 DEG C, and rotating speed 200 ~ 220rpm, constant temperature culture 8 ~ 12h, fermented liquid concentration OD
600reach 0.8 ~ 1, obtain fermented liquid.
(2) bacterium liquid mixing
Step (1) gained fermented liquid is pressed Bacillus cereus: bacillus amyloliquefaciens: bacillus megaterium: subtilis volume ratio=3:7:5:8 mixing.
(3) bacterium powder is prepared
By the centrifugal 8min of step (2) gained mixed fermentation liquid 8000rpm, add protective material.Every 10mg bacterium mud, joins in the skim-milk of 30ml 0.2g/ml, adds the sucrose solution of 6ml 1g/ml, 2ml 0.3g/ml Pidolidone sodium solution, 2ml 0.2g/ml vitamin c solution after fully mixing.Stirrer is adopted fully to mix.
To add protectant bacterium mud to spend the night-80 DEG C of refrigerator pre-freezes, then it is dry to put into vacuum freeze drier.
Beneficial effect:
1, the microbiobacterial agent of decrement mud of the present invention can make mud decrement reach 28% after processing mud, and water content reduces 30%.
2, the form of microbial inoculum of the present invention active sludge after using there occurs certain change, in active sludge, macrobead amount of sludge is more, structure is tightr. the change of the form of active sludge, physical structure layer and the distribution of varying environment condition is formed in mud flco, different microorganism dominant microfloras is made to have had suitable living environment, be conducive to different microorganisms flora and form dominant microflora in activated sludge floc, promote that the function of active sludge changes.
Embodiment:
Embodiment 1: a kind of preparation method of microbiobacterial agent of decrement mud
(1) preparation of fermented liquid
Bacillus cereus, bacillus amyloliquefaciens, bacillus megaterium and subtilis are cultivated respectively as follows:
Seed culture: by inclined plane inoculating in seed culture medium, in 36 DEG C, rotating speed 260rpm cultivates 2 days,
The composition of seed culture medium: extractum carnis 0.5%, sodium-chlor 0.5%, extractum carnis 1%, insufficient section is with distilled water polishing, and pH regulator scope is 7.2;
Fermentation culture: nutrient media components is identical with seed culture medium, accesses fermention medium by seed liquor by inoculum size 2%, in 36 DEG C, and rotating speed 210rpm, constant temperature culture 10h, fermented liquid concentration OD
600reach 0.8, obtain fermented liquid;
Described bacillus cereus deposit number is CGMCC1.8641;
Described bacillus amyloliquefaciens deposit number is CGMCC1.1226;
Described bacillus megaterium deposit number is CGMCC1.234;
Described subtilis deposit number is CGMCC1.1630.
(2) bacterium liquid mixing
Step (1) gained fermented liquid is pressed Bacillus cereus: bacillus amyloliquefaciens: bacillus megaterium: subtilis volume ratio=3:7:5:8 mixing.
(3) bacterium powder is prepared
Step (2) gained mixed fermentation liquid is added protective material postlyophilization and obtains bacterium powder.By the centrifugal 8min of step (2) gained mixed fermentation liquid 8000rpm, add protective material.Every 10mg bacterium mud, joins in the skim-milk of 30ml 0.2g/ml, adds the sucrose solution of 6ml 1g/ml, 2ml 0.3g/ml Pidolidone sodium solution, 2ml 0.2g/ml vitamin c solution after fully mixing.Stirrer is adopted fully to mix.
To add protectant bacterium mud to spend the night-80 DEG C of refrigerator pre-freezes, then it is dry to put into vacuum freeze drier
Obtain bacterium powder.
Embodiment 2: a kind of application of microbiobacterial agent of decrement mud
Embodiment 1 gained bacterium powder is pressed mass volume ratio 0.03% addition, to be added in Jinnan District, Tianjin doube bridge sewage work oxidation ditch in active sludge, active sludge sludge concentration after sample concentration reaches 50g/L, water ratio 85.97%, in 30 DEG C, 80r shaking table condition Imitating envrionment conditions carries out process 72h, and mud decrement reaches 16.75%, and water ratio is down to 66.87%.
Embodiment 3: a kind of application of microbiobacterial agent of decrement mud
By embodiment 1 gained bacterium powder by 0.04% addition, be added in the active sludge come by the collection of Jinnan District, Tianjin doube bridge sewage work, active sludge sludge concentration after sample concentration reaches 50g/L, water ratio 85.97%, in 30 DEG C, 80r shaking table condition Imitating envrionment conditions carries out process 72h, and mud decrement reaches 25.32%, and water ratio is down to 57.32%.
Embodiment 4: a kind of application of microbiobacterial agent of decrement mud
By embodiment 1 gained bacterium powder by 0.05% addition, be added in the active sludge come by the collection of Jinnan District, Tianjin doube bridge sewage work, active sludge sludge concentration after sample concentration reaches 50g/L, water ratio 85.97%, in 30 DEG C, 80r shaking table condition Imitating envrionment conditions carries out process 72h, and mud decrement reaches 27.75%, and water ratio is down to 56.65%.
The form observing active sludge in experimentation there occurs certain change, in active sludge, macrobead amount of sludge is more, structure is tightr. the change of the form of active sludge, physical structure layer and the distribution of varying environment condition is formed in mud flco, different microorganism dominant microfloras is made to have had suitable living environment, be conducive to different microorganisms flora and form dominant microflora in activated sludge floc, promote that the function of active sludge changes.
Claims (7)
1. the microbiobacterial agent of a decrement mud; it is characterized in that; described microbial inoculum by Bacillus cereus, bacillus amyloliquefaciens, bacillus megaterium and subtilis fermented liquid by volume 3:7:5:8 form, and be equipped with protective material and make bacterium powder through lyophilize.
2. the microbiobacterial agent of a kind of decrement mud as claimed in claim 1, is characterized in that, described bacillus cereus deposit number is CGMCC No.1.8641.
3. the microbiobacterial agent of a kind of decrement mud as claimed in claim 1, is characterized in that, described bacillus amyloliquefaciens deposit number is CGMCC No.1.1226.
4. the microbiobacterial agent of a kind of decrement mud as claimed in claim 1, is characterized in that, described bacillus megaterium deposit number is CGMCC No.1.234.
5. the microbiobacterial agent of a kind of decrement mud as claimed in claim 1, is characterized in that, described subtilis deposit number is CGMCC No.1.1630.
6. the microbiobacterial agent of a kind of decrement mud as claimed in claim 1, is characterized in that, it is 0.03% ~ 0.05% of sludge volume that described microbiobacterial agent adds quality.
7. the microbiobacterial agent of a kind of decrement mud as claimed in claim 1, it is characterized in that, the preparation method of described microbiobacterial agent is as follows:
(1) preparation of fermented liquid
Bacillus cereus, bacillus amyloliquefaciens, bacillus megaterium and subtilis are cultivated respectively as follows:
Seed culture: by inclined plane inoculating in seed culture medium, in 35 ~ 37 DEG C, rotating speed 200 ~ 220rpm cultivates 1 ~ 2 day,
The composition of seed culture medium: extractum carnis 0.5%, sodium-chlor 0.5%, extractum carnis 1%, insufficient section is with distilled water polishing, and pH regulator scope is 7.2 ~ 7.4;
Fermentation culture: nutrient media components is identical with seed culture medium, accesses fermention medium by seed liquor by inoculum size 1 ~ 2%, in 35 ~ 37 DEG C, and rotating speed 200 ~ 220rpm, constant temperature culture 8 ~ 12h, fermented liquid concentration OD
600reach 0.8 ~ 1, obtain fermented liquid;
(2) bacterium liquid mixing
Step (1) gained fermented liquid is pressed Bacillus cereus: bacillus amyloliquefaciens: bacillus megaterium: subtilis volume ratio=3:7:5:8 mixing;
(3) bacterium powder is prepared
Step (2) gained mixed fermentation liquid is added protective material postlyophilization and obtains bacterium powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410835555.8A CN104805032A (en) | 2014-12-29 | 2014-12-29 | Multifunctional microbial agent for sludge reduction and preparation thereof |
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| CN201410835555.8A CN104805032A (en) | 2014-12-29 | 2014-12-29 | Multifunctional microbial agent for sludge reduction and preparation thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154357A (en) * | 2015-08-18 | 2015-12-16 | 上海交通大学 | Preparation method and application of secondary-salinization soil remediation microbial inoculum |
| CN105385648A (en) * | 2015-12-09 | 2016-03-09 | 天津北洋百川生物技术有限公司 | Screening, preparation, and application method of microbial composite inoculant for reducing residual sludge |
| CN107759042A (en) * | 2016-08-19 | 2018-03-06 | 辽宁惠源生物环保科技有限公司 | A kind of complex micro organism fungicide for mud decrement and preparation method thereof |
| CN108585224A (en) * | 2018-05-21 | 2018-09-28 | 山东省农业科学院农业资源与环境研究所 | A method of using complex micro organism fungicide treated sewage and being reduced sludge generation |
| CN112899207A (en) * | 2021-04-23 | 2021-06-04 | 重庆融极浩瀚生物技术有限公司 | Compound microbial agent and preparation method and application thereof |
| CN114684927A (en) * | 2020-12-31 | 2022-07-01 | 中国石油化工股份有限公司 | Method for quickly starting denitrification function of sewage treatment system |
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| CN103058476A (en) * | 2012-12-31 | 2013-04-24 | 天津北洋百川生物技术有限公司 | Novel treatment method for excess sludge |
| CN103725737A (en) * | 2013-12-27 | 2014-04-16 | 天津北洋百川生物技术有限公司 | Method for producing sludge protein by treating surplus sludge by biological method |
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Patent Citations (4)
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154357A (en) * | 2015-08-18 | 2015-12-16 | 上海交通大学 | Preparation method and application of secondary-salinization soil remediation microbial inoculum |
| CN105154357B (en) * | 2015-08-18 | 2018-06-08 | 上海交通大学 | The preparation method and application of secondary salinization soil remediation microbial inoculum |
| CN105385648A (en) * | 2015-12-09 | 2016-03-09 | 天津北洋百川生物技术有限公司 | Screening, preparation, and application method of microbial composite inoculant for reducing residual sludge |
| CN107759042A (en) * | 2016-08-19 | 2018-03-06 | 辽宁惠源生物环保科技有限公司 | A kind of complex micro organism fungicide for mud decrement and preparation method thereof |
| CN108585224A (en) * | 2018-05-21 | 2018-09-28 | 山东省农业科学院农业资源与环境研究所 | A method of using complex micro organism fungicide treated sewage and being reduced sludge generation |
| CN114684927A (en) * | 2020-12-31 | 2022-07-01 | 中国石油化工股份有限公司 | Method for quickly starting denitrification function of sewage treatment system |
| CN114684927B (en) * | 2020-12-31 | 2023-09-01 | 中国石油化工股份有限公司 | Method for quickly starting denitrification function of sewage treatment system |
| CN112899207A (en) * | 2021-04-23 | 2021-06-04 | 重庆融极浩瀚生物技术有限公司 | Compound microbial agent and preparation method and application thereof |
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Application publication date: 20150729 |