CN112899207A - Compound microbial agent and preparation method and application thereof - Google Patents
Compound microbial agent and preparation method and application thereof Download PDFInfo
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- CN112899207A CN112899207A CN202110441220.8A CN202110441220A CN112899207A CN 112899207 A CN112899207 A CN 112899207A CN 202110441220 A CN202110441220 A CN 202110441220A CN 112899207 A CN112899207 A CN 112899207A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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Abstract
The invention relates to the technical field of sewage treatment, and particularly relates to a compound microbial agent as well as a preparation method and application thereof. The composite microbial agent comprises the following components in a volume ratio of 2-3: 2-3: 1-2: 1.5-2: 1-1.5: 1-2 of bacillus megatherium, lactobacillus paracasei, bacillus licheniformis, paenibacillus curvulosus, pediococcus pentosaceus and rhodococcus erythropolis. The compound microbial agent provided by the invention is used for sewage treatment and comprises the following components: enhancing the pollutant removal effect of the activated sludge; secondly, the performance of the sludge is improved, and the flocculation property of the activated sludge is enhanced; thirdly, the mud floating phenomenon of the secondary sedimentation tank is reduced, and the effluent quality is improved; and fourthly, the function of effectively inhibiting and preventing the filamentous fungi from swelling.
Description
Technical Field
The invention relates to the technical field of sewage treatment, and particularly relates to a compound microbial agent as well as a preparation method and application thereof.
Background
The domestic sewage is turbid, dark and odorous liquid generated in daily life of people, is slightly alkaline and mainly comprises two parts of excrement water and washing water. The domestic sewage generally does not contain toxic substances, mainly comprises some non-toxic organic matters, the content of which is about 60 percent, mainly comprises saccharides, starch, cellulose, grease, protein, urea and the like, and the organic matters in all suspended matters almost account for more than three quarters of the total amount. The domestic sewage contains high content of nitrogen, phosphorus and sulfur, is accompanied by various detergents and also contains a considerable amount of microorganisms including pathogens such as viruses, parasites and the like. If the waste water is discharged without treatment, the waste water has serious influence on the environment and the human health.
Along with the development of social economy and rapid increase of population quantity in China, the discharge amount of domestic sewage is increased sharply, and the percentage of the total amount of the sewage discharge is increased year by year. Domestic sewage treatment has become the key point of water pollution treatment in China. The active sludge method is a main method for treating domestic sewage at present, and the active sludge has to have good organic matter degradation function and sedimentation performance to effectively treat the domestic sewage. When the activated sludge is deteriorated in performance and conditions such as sludge bulking, scattered growth, needle-shaped sludge, sludge floating and the like occur, the problems of deterioration of effluent quality, environmental pollution and increase of operation power cost are inevitably caused. The prior art urgently needs an activated sludge ecological balance which can effectively improve the sludge performance and stabilize the whole system; the removal effect is enhanced, and water pollutants are degraded; the sludge sedimentation effect is enhanced, the effluent is clearer, and the defects of the prior art are overcome by using substances which reach the first-grade A standard of pollutant discharge of urban sewage treatment plants.
Disclosure of Invention
The invention aims to provide a compound microbial agent and a preparation method and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of a compound microbial agent, which comprises the following steps:
and mixing the bacillus megaterium fermentation liquor, the lactobacillus paracasei fermentation liquor, the bacillus licheniformis fermentation liquor, the paenibacillus curdling fermentation liquor, the pediococcus pentosaceus fermentation liquor and the rhodococcus erythropolis fermentation liquor to prepare the compound microbial agent.
Preferably, the volume ratio of the bacillus megaterium fermentation liquid to the lactobacillus paracasei fermentation liquid to the bacillus licheniformis fermentation liquid to the paenibacillus decondens fermentation liquid to the pediococcus pentosaceus fermentation liquid to the rhodococcus erythropolis fermentation liquid is 2-3: 1-2: 1.5-2: 1-1.5: 1-2.
Preferably, the number of bacteria in the bacillus megatherium fermentation liquid, the lactobacillus paracasei fermentation liquid, the bacillus licheniformis fermentation liquid, the paenibacillus disaggregation fermentation liquid, the pediococcus pentosaceus fermentation liquid and the rhodococcus erythropolis fermentation liquid is more than or equal to 1 multiplied by 108CFU/g。
The invention also provides the compound microbial agent prepared by the preparation method.
The invention also provides application of the compound microbial agent in the aspect of treating domestic sewage.
Preferably, the microbial inoculum is used alone or prepared into compound microbial sludge for use when treating domestic sewage.
Preferably, the method used alone is: the microbial inoculum is directly thrown into domestic sewage.
Preferably, the method for preparing the composite microbial bacterial sludge comprises the following steps:
(1) inoculating the microbial inoculum into domestic sewage, and performing fermentation culture to obtain a first fermentation product;
(2) and (3) adding sludge into the first fermentation product, and continuously fermenting for 16-24 hours to obtain the composite microbial sludge.
Preferably, the volume ratio of the inoculated microbial inoculum in the step (1) to the domestic sewage is 3-5: 10;
the domestic sewage in the step (1) is domestic sewage with suspended matter content less than or equal to 20 mg/L;
the time for throwing the sludge in the step (2) is that the number of the microorganisms in the first fermentation product is more than or equal to 1 multiplied by 108CFU/g;
the ratio of the volume of the sludge added in the step (2) to the total volume of the domestic sewage and the microbial inoculum is 3-4.5: 10.
The invention provides a compound microbial agent and a preparation method and application thereof, and the compound microbial agent has the following advantages:
(1) the pollutant removal effect of the activated sludge is enhanced;
(2) the sludge performance is improved, and the flocculation property of the activated sludge is enhanced;
(3) the mud floating phenomenon of the secondary sedimentation tank is reduced, and the effluent quality is improved;
(4) effectively inhibit and prevent the expansion of the filamentous fungi.
Drawings
FIG. 1 is a flocculation diagram of the complex microbial agent (the left diagram is without the complex microbial agent, and the right diagram is with the complex microbial agent).
FIG. 2 shows the properties of the sludge before feeding (wherein the left image is taken from the front end of the aerobic tank and the right image is taken from the rear end of the aerobic tank).
FIG. 3 shows the properties of the sludge 5 days after the charging (wherein the left image is taken from the front end of the aerobic tank and the right image is taken from the rear end of the aerobic tank).
FIG. 4 shows the properties of the sludge 15 days after the charging (wherein the left image is taken from the front end of the aerobic tank and the right image is taken from the rear end of the aerobic tank).
Detailed Description
The invention provides a preparation method of a compound microbial agent, which comprises the following steps:
and mixing the bacillus megaterium fermentation liquor, the lactobacillus paracasei fermentation liquor, the bacillus licheniformis fermentation liquor, the paenibacillus curdling fermentation liquor, the pediococcus pentosaceus fermentation liquor and the rhodococcus erythropolis fermentation liquor to prepare the compound microbial agent.
In the invention, the volume ratio of the bacillus megaterium fermentation liquid to the lactobacillus paracasei fermentation liquid to the bacillus licheniformis fermentation liquid to the paenibacillus coagulans fermentation liquid to the pediococcus pentosaceus fermentation liquid to the rhodococcus erythropolis fermentation liquid is 2-3: 1-2: 1.5-2: 1-1.5: 1-2, preferably 2.5:2.5:1.5:1.75:1.25: 1.5.
In the invention, the number of bacteria in the bacillus megatherium fermentation liquor, the lactobacillus paracasei fermentation liquor, the bacillus licheniformis fermentation liquor, the paenibacillus coagulans fermentation liquor, the pediococcus pentosaceus fermentation liquor and the rhodococcus erythropolis fermentation liquor is more than or equal to 1 multiplied by 108CFU/g。
In the present invention, the Bacillus megaterium, Lactobacillus paracasei, Bacillus licheniformis, Paenibacillus coagulans, Pediococcus pentosaceus and Rhodococcus erythropolis are all commercially available.
The invention also provides the compound microbial agent prepared by the preparation method.
The invention also provides application of the compound microbial agent in the aspect of treating domestic sewage.
In the invention, the microbial inoculum is used alone or prepared into compound microbial sludge for use when treating domestic sewage.
In the present invention, the method used alone is: the microbial inoculum is directly thrown into domestic sewage.
In the invention, the method for preparing the composite microbial bacterial sludge comprises the following steps:
(1) inoculating the microbial inoculum into domestic sewage, and performing fermentation culture to obtain a first fermentation product;
(2) and (3) adding sludge into the first fermentation product, and continuously fermenting for 16-24 hours to obtain the composite microbial sludge.
In the invention, the volume ratio of the inoculated microbial inoculum in the step (1) to the domestic sewage is 3-5: 10, preferably 4: 10;
the domestic sewage in the step (1) is domestic sewage with suspended matter content less than or equal to 20 mg/L;
the time for throwing the sludge in the step (2) is that the number of the microorganisms in the first fermentation product is more than or equal to 1 multiplied by 108CFU/g;
the ratio of the volume of the sludge added in the step (2) to the total volume of the domestic sewage and the microbial inoculum is 3-4.5: 10, and preferably 4: 10.
In the invention, the continuous fermentation in the step (2) is preferably carried out for 16-24 h, more preferably for 18-22 h, and even more preferably for 20 h.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
0.5g of kaolin was weighed into a 50mL cuvette and tap water was added to the scale. After shaking up, 1ml of compound microbial inoculum is added, the pH is adjusted to 8.5, the mixture is stirred rapidly for 1 minute by a glass rod, stirred slowly for 2 minutes and kept stand for 10 minutes. Meanwhile, kaolin suspension without the compound microbial agent is used as a reference solution. And (3) taking the supernatant, measuring the absorbance at 500nm of a 721 type ultraviolet spectrophotometer, and detecting the flocculation efficiency of the compound microbial agent on the kaolin, wherein the result is shown in figure 1.
The kaolin is kaolin which passes through a 1250-mesh sieve.
The compound microbial agent is prepared from bacillus megatherium fermentation liquor, lactobacillus paracasei fermentation liquor, bacillus licheniformis fermentation liquor, paenibacillus coagulans fermentation liquor, pediococcus pentosaceus fermentation liquor and rhodococcus erythropolis fermentation liquor according to the volume ratio of 3:2:1:2:1.5: 2.
The number of bacteria in the bacillus megatherium fermentation liquid, the lactobacillus paracasei fermentation liquid, the bacillus licheniformis fermentation liquid, the paenibacillus curdling fermentation liquid, the pediococcus pentosaceus fermentation liquid and the rhodococcus erythropolis fermentation liquid is 1 multiplied by 108CFU/g。
The flocculation efficiency upsilon ═ AOD500-BOD500)/AOD500 x 100%; AOD500 is the measured value of the supernatant in the control solution, and BOD500 is the measured value of the supernatant after adding the complex microbial agent.
Through measurement and calculation, the flocculation efficiency of the compound microbial agent is 92.13%.
Example 2
The daily water treatment amount of the Orthos Hami sewage treatment plant is 3000m3D, i.e. an amount of water treated per hour of 125m3The total effective volume of the biochemical pool is 1800m by adopting the A/O process on site3。
Preparing one 8-ton fermentation tank with an aeration and stirring device, and extracting domestic sewage passing through an on-site coarse grating and a fine grating for 3m3Is filteredA device for ensuring that the suspended matters in the filtered domestic sewage water body are less than 20mg/L for pumping, then placing the domestic sewage water body into a fermentation tank for standby, starting an aeration and stirring device of the fermentation tank, and adding 1.2m of the compound microbial inoculum of the embodiment 13Performing fermentation culture, sampling and counting every 12 hours, and when the number of microorganisms is 1 × 108When CFU/g is needed, 1.7m of sludge in the on-site biochemical sludge sedimentation tank is extracted3And (4) carrying out aeration and stirring culture again in the fermentation tank, culturing for 18 hours to obtain the compound microorganism bacterial sludge, and closing aeration and stirring for later use.
The sludge concentration of the biochemical tank before the feeding is 2700mg/L, the sludge discharge operation is carried out on the secondary sedimentation tank of the biochemical tank at the moment, and the sludge concentration in the aerobic tank is controlled to 2200 mg/L. Starting a stirring and aerating device of the fermentation tank so as to fully and uniformly mix the bacterial sludge, starting a bacterial sludge adding switch, and controlling the flow rate to be 0.8m3And h, adding the composite microbial sludge prepared on site into a water inlet of the pool A (facultative tank) according to the proportion of 0.32 percent of the total effective volume of the pool A and the pool O of the biochemical tank (namely the total effective volume of the aerobic tank and the facultative tank), wherein the adding time is 7.3 hours after the adding is finished. After the addition is finished, the biochemical treatment sludge is started to flow back by 100 percent, and the sludge discharge operation of a secondary sedimentation tank of the on-site biochemical tank can not be carried out within two days. On the third day, the amount of water treated by the biochemical system was 125m3The metering pump is started and the flow of the metering pump is controlled to be 0.025m by taking the flow/h as the reference3The complex microbial inoculant of example 1 was dosed at the internal reflux inlet of the biochemical treatment unit.
And observing the properties of the sludge in the aerobic tank before feeding, 5 days and 15 days after feeding, and obtaining results shown in figures 2-4.
The indexes of COD, ammonia nitrogen, total nitrogen and total phosphorus in the water before the feeding, 1 day after the feeding, 2 days and 6 days after the feeding are measured, and the results are shown in a table 1.
The post-feeding means that the last time of feeding the compound microbial agent begins to calculate.
TABLE 1 quality index of effluent from two sedimentation tanks before and after adding composite microorganism bacterial sludge and microbial inoculum
Table 1 shows: the compound microbial agent is added, so that the sludge performance can be effectively improved, and the ecological balance of the activated sludge of the whole system is stabilized; the removal effect is enhanced, and water pollutants are degraded; the sludge sedimentation effect is enhanced, the effluent is clearer, and the first-grade A standard of pollutant discharge of urban sewage treatment plants is met.
FIGS. 2-4 show: the biological situation after adding the compound microbial agent is as follows: in SV30 sedimentation detection, the sludge sedimentation speed is high, the sludge does not swell, the suspended matters in the supernatant are obviously reduced greatly compared with those in the supernatant before the sludge is added, the filamentous fungi are effectively inhibited, and the proportion of the filamentous fungi is maintained in a normal range of about 20 percent; the microscopic examination of the sludge shows that the stacking property of the zoogloea becomes strong, and simultaneously, a large number of sessile protozoa such as coccinella, branchlet, singly-shrinked protozoa and the like appear on the zoogloea, which shows that the activated sludge is good in ecology, strong in sludge activity, good in water quality treatment effect and clear in effluent. Meanwhile, the system can still keep good activity and sludge ecological balance under the impact of high COD and high ammonia nitrogen of inlet water, and the water quality data completely reach the standard, which shows that the impact load resistance of the biochemical system can be obviously improved by the biological agent.
The above embodiments show that the invention provides a compound microbial agent, a preparation method and an application thereof, and the compound microbial agent has the following advantages:
(1) the pollutant removal effect of the activated sludge is enhanced;
(2) the sludge performance is improved, and the flocculation property of the activated sludge is enhanced;
(3) the mud floating phenomenon of the secondary sedimentation tank is reduced, and the effluent quality is improved;
(4) effectively inhibit and prevent the expansion of the filamentous fungi.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. The preparation method of the compound microbial agent is characterized by comprising the following steps:
and mixing the bacillus megaterium fermentation liquor, the lactobacillus paracasei fermentation liquor, the bacillus licheniformis fermentation liquor, the paenibacillus curdling fermentation liquor, the pediococcus pentosaceus fermentation liquor and the rhodococcus erythropolis fermentation liquor to prepare the compound microbial agent.
2. The preparation method according to claim 1, wherein the volume ratio of the bacillus megaterium fermentation liquid, the lactobacillus paracasei fermentation liquid, the bacillus licheniformis fermentation liquid, the paenibacillus coagulans fermentation liquid, the pediococcus pentosaceus fermentation liquid and the rhodococcus erythropolis fermentation liquid is 2-3: 1-2: 1.5-2: 1-1.5: 1-2.
3. The method according to claim 1 or 2, wherein the number of bacteria in the Bacillus megaterium fermentation broth, the Lactobacillus paracasei fermentation broth, the Bacillus licheniformis fermentation broth, the Paecilomyces decondens fermentation broth, the Pediococcus pentosaceus fermentation broth, and the Rhodococcus erythropolis fermentation broth is 1X 10 or more in number8CFU/g。
4. The complex microbial inoculant prepared by the preparation method of any one of claims 1 to 3.
5. The use of the complex microbial inoculant of claim 4 for treating domestic sewage.
6. The use of claim 5, wherein the microbial inoculum is used alone or prepared into compound microbial sludge for use in domestic sewage treatment.
7. The use according to claim 6, characterized in that the method used alone is: the microbial inoculum is directly thrown into domestic sewage.
8. The use of claim 6, wherein the method for preparing the composite microbial sludge comprises the following steps:
(1) inoculating the microbial inoculum into domestic sewage, and performing fermentation culture to obtain a first fermentation product;
(2) and (3) adding sludge into the first fermentation product, and continuously fermenting for 16-24 hours to obtain the composite microbial sludge.
9. The application of the microbial inoculum, according to the claim 8, wherein the ratio of the volume of the inoculated microbial inoculum in the step (1) to the volume of domestic sewage is 3-5: 10;
the domestic sewage in the step (1) is domestic sewage with suspended matter content less than or equal to 20 mg/L;
the time for throwing the sludge in the step (2) is that the number of the microorganisms in the first fermentation product is more than or equal to 1 multiplied by 108CFU/g;
the ratio of the volume of the sludge added in the step (2) to the total volume of the domestic sewage and the microbial inoculum is 3-4.5: 10.
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| CN113136351A (en) * | 2021-04-25 | 2021-07-20 | 重庆融极浩瀚生物技术有限公司 | Composite microbial flocculant and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113136351A (en) * | 2021-04-25 | 2021-07-20 | 重庆融极浩瀚生物技术有限公司 | Composite microbial flocculant and preparation method and application thereof |
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