CN107759042A - A kind of complex micro organism fungicide for mud decrement and preparation method thereof - Google Patents

A kind of complex micro organism fungicide for mud decrement and preparation method thereof Download PDF

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Publication number
CN107759042A
CN107759042A CN201610688116.8A CN201610688116A CN107759042A CN 107759042 A CN107759042 A CN 107759042A CN 201610688116 A CN201610688116 A CN 201610688116A CN 107759042 A CN107759042 A CN 107759042A
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ponds
iat
microbial inoculum
dat
sludge
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赵飞
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Liaoning Huiyuan Biological Environmental Protection Technology Co Ltd
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Liaoning Huiyuan Biological Environmental Protection Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C02F11/04—Anaerobic treatment; Production of methane by such processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor

Abstract

The invention discloses a kind of complex micro organism fungicide for mud decrement, the complex micro organism fungicide contains：Flocculate bacteria agent, drop albumen bacteria agent and lipid-loweringing bacteria agent.Complex micro organism fungicide is applied to the DAT IAT process sections of sewage disposal, and described drop albumen microbial inoculum and lipid-loweringing microbial inoculum are added in DAT ponds by its specific method to put into complex micro organism fungicide in DAT ponds and IAT ponds respectively；Described flocculation microbial inoculum is added in IAT ponds；And DAT ponds will be back to after 20% sludge taking-up progress ultrasonication in IAT ponds.The present invention puts into lipid-loweringing bacteria agent and drop albumen bacteria agent in DAT ponds, realize the thalline dead in degrading activity sludge or aging while degradation of organic substances, in the case where not influenceing effluent quality, the generating capacity of excess sludge is reduced by reaching to the process decrement of sludge.

Description

A kind of complex micro organism fungicide for mud decrement and preparation method thereof
Technical field
The present invention relates to technical field of sludge treatment, specially a kind of complex micro organism fungicide for mud decrement and its Preparation method.
Background technology
Activated sludge process has technology maturation, technique as the dirty water living creature processing technique being most widely used in the world The features such as species is more, wastewater treatment efficiency is good, but activated sludge process also brings a large amount of by-products while sewage is handled Thing residual active sludge one by one, sludge quantity generally account for the 0.3%-0.5% (volume) or about sewage load of sewage quantity 1%-2% (quality)；If belonging to advanced treating, sludge quantity can also increase 0.5-1 times, and sewage load and discharge standard carry Height, necessarily cause the increase of amount of sludge.
It is caused surplus in corresponding sewage disposal process as China cities and towns and Industrial Waste Water Treatments ability are greatly enhanced Remaining sludge quantity is also increasingly huge, and annual 10%-15% speed increase, sludge pollution are on the rise, and sludge handling problem is convex It is aobvious.The current sludge in China is mainly with compost, landfill and the formal layout arbitrarily transported outward, and in mix, its ratio is substantially Compost 10%, landfill 20%, arbitrarily the simple landfill of outward transport and stacking account for 70%.Even if existing sewage treatment plant is carried out to sludge Concentration, but because the limitation of technology and sludge dewatering difficulty does not often reach requirement, moisture content is up to 80% wet mud Transport difficulty is not only added, and the environment on transit route periphery is faced threat, while is made troubles to follow-up disposal. If caused excess sludge during processing sewage is not disposed, the pollutant such as pathogenic microorganism contained therein, heavy metal Transfer to and secondary pollution is caused in surrounding environment.
Concern of the people to sludge handling problem focuses mostly on to be added in end treatment, the seldom process control to sludge generating capacity With research.The Sludge Reduction Technologies studied both at home and abroad at present mainly have ozonation technology, and ultrasonic disruption cell technology, solution is occasionally Connection technology, soda acid dissolving technology, but cost height or technical bottleneck all be present, do not form certain market application.At present The huge sludge output of sewage treatment plant has highlighted with the incomplete contradiction of sludge disposal technology.If do not control excess sludge Yield, sludge is reduced from source, but only take end treatment, then which kind of Treatment of Sludge or disposal skill no matter taken Art, all it is difficult that sludge problem is resolved.
The most frequently used processing method of disposal of excess sludge be concentration, anaerobic digestion, mechanical dehydration, desiccation, compost, burning and Landfill etc..The quantitative reduction method of sludge mainly has the modes such as thickening, anaerobic digestion, burning.Sludge is carried out with dewatering type The method of volume reduction, is only the reduction of the water content of sludge, and the solids content of sludge is not reduced, it is impossible to realizes the decrement of sludge Change.Equipment requirement needed for anaerobic digestion processing excess sludge is high, investment is big, long processing period.Burning method handles excess sludge Complex process, power consumption height, waste gas from incinerator easily cause secondary pollution.A variety of sludge reduction New Method for Processing are developed in recent years, Wherein, SBR methods are the political reforms of activated sludge process, are to purify sewage, this point SBR by the activity of active sludge microorganism Technique is completely the same with conventional activated sludge process, and simply the method for operation is different, and traditional handicraft uses continuously-running duty, sewage Continuously enter processing system and continuously discharge, the function in system per unit is constant, and sewage flows successively through each unit, so as to complete Processing procedure.SBR techniques typically use intermittent duty mode, and sewage interval enters processing system and interval is discharged.In system only If a processing unit, the unit plays different effects in different time, and sewage is carried out in chronological order after entering the unit The processing of difference in functionality.
Compared with other techniques, SBR techniques at home and abroad obtain in recent years due to having the advantages that simple, efficient, low consumption Relatively broad application, but some technical problems present in SBR techniques are somewhat limited its application.It is first First, SBR techniques are intermittent water inflow, intermittent aerating, and this not only reduces the utilization rate of aeration phase reaction tank, and blast aeration Machine is due to intermittent running, and its nominal air delivery and power are also higher, and the operation of whole technique becomes to be not sufficiently stable；Second, it is former dirty Water interval enters people's reaction tank, it is necessary to install complex order water inlet gate valve and robot control system(RCS)；3rd, when inflow is larger When, SBR techniques need more set reaction tank parallel runnings, add the complexity of system；4th, dropped for the hardly possible of some high concentrations Solving organic wastewater needs the longer reaction time.
In order to solve problem above, DAT-IAT techniques set training wall in same reaction tank, front and rear point of training wall Zuo Wei not DAT ponds and IAT ponds.Raw sewage continuously enters DAT ponds and continuous aeration, and waterpower equilibrium is served to whole processing system Effect, significantly improve the stability of system operation, when particularly handling high concentrated organic wastewater, continuous aeration strengthens pair The degraded of hardly degraded organic substance, relative to shorten the cycle of operation, the nominal air delivery and power for making blast aeration machine are also reduced therewith； And the continuum micromeehanics in DAT ponds, the operation is can be achieved with using common sewage pump, complicated order water inlet need not be used completely Gate valve and robot control system(RCS).Therefore, DAT-IAT techniques not only possess the plurality of advantages of SBR techniques, and solve SBR techniques institute The problem of existing, being one is worth research, the new technology promoted.
DAT-IAT technique main body structures are made up of the reaction tank of two series connection, i.e. aerobic basin (Demand Aeration Tank) and intermittent aerating pond (Intermittent Aeration Tank), generally DAT continuum micromeehanics, Continuous aeration (also can intermittent aerating), it is the main place of microbial degradation organic matter in fluidised form is thoroughly mixed in pond；IAT connects Continuous water inlet, intermittent aerating further remove the organic matter in sewage, complete the processes such as reaction, precipitation, water outlet in IAT, make sewage Reach discharge standard, water and residual active sludge are discharged by IAT after processing
In DAT-IAT techniques, the elementary reaction first occurs at DAT, and the pond whole day continuously exposes while continuum micromeehanics Gas, for current in fluidised form is thoroughly mixed, its main task is the absorption for completing activated sludge to organic matter in pond.Sewage start with In short period after activated sludge contact, the organic pollution in water inlet is largely removed, and completes physical absorption and biology Adsorb, be in suspend to be removed by activated sludge cohesion and absorption with the organic pollution of colloidal state in sewage, and inhaled The organic matter for being attached to microbial cell surface can be progressively taken the photograph in human body by microorganism.Because the system is continuum micromeehanics, to whole Reaction system plays the balanced effect of waterpower.The part 2 of reaction occurs in IAT, after preliminary biological treatment is carried out through DAT Sewage continuously enters IAT by double-deck water dispensing apparatus between two ponds, and the exposure of certain time is carried out by technology Calculation requirement Gas or stirring, so as to reach the purpose of aerobic reaction (removing remaining BOD5 and nitrification).In general, DAT and L＆T oxygen demands The ratio between be 65%：35%, sometimes to reach more preferable sedimentation effect, it is aerated before precipitation in the shortest time, it is attached to remove The nitrogen on sludge.The active sludge microorganism survived in IAT is continued to complete organic dirt in surrounding environment sewage Dye thing is absorbed as nutrition, absorbed, further oxidation Decomposition and anabolic process, and by anabolite one by one Excess sludge is from IAT ponds discharge system.
DAT-IAT systems can be considered extended aeration, and precipitation occurs in IAT, and its active sludge intermixture has light, wadding Body particle is small, is easily taken by water outlet, easily by disturbance the features such as, therefore for avoid precipitation process produce disturbance, need in the design by Flow velocity during DAT injections IAT sets very low.After IAT stops being aerated, active pollution index static state deposition and supernatant Liquid separates.DAT-IAT techniques using unique double partition wall flow guide systems, effectively prevent sewage from DAT inflow IAT when, go out The existing short phenomenon for flowing or disturbing settled sludge blanket of waterpower, thus there is good sedimentation effect.IAT active sludge intermixtures Mass concentration between 2~4mg/L, there is flocculating property, can produce layered precipitation, have between muddy water during precipitation more visible Interface, floccule body forms entirety, it is common sink, reach clarified supernatant, concentrate the effect of mixed liquor.
DAT-IAT technique basic operation operation programs are as follows：
(1) intake：Sewage continuously enters DAT ponds after continuous aeration, is entered by parallel works between DAT ponds and IAT ponds IAT ponds.The not direct emission treatment water in DAT ponds, therefore unlike continuously entering the activated sludge process of continuous effluent easily by load variations Influence.
(2) react：Reaction process divides two parts to carry out.DAT ponds are first occurred at, the pond is continuous while continuum micromeehanics Aeration.The mechanism of organics removal and operation are identical with continuous stream activated sludge process.The Part II of reaction process occurs in IAT Pond, the sewage through the preliminary biological treatment in DAT ponds continuously enter IAT.The aeration for carrying out certain time is set to reach by technique The purpose of oxygen.
(3) precipitate：Precipitation process occurs over just IAT ponds.After stopping aeration when IAT ponds, active pollution index starts gravity Precipitation and mud-water separation.The precipitation process in IAT ponds is equivalent to the secondary sedimentation tank function in continuous stream activated sludge process.
(4) draining：Drainage procedure only occurs in IAT ponds.IAT ponds pool water level reaches peak level, and by depositing technology with Afterwards, supernatant is slowly discharged outside pond by the water decanter for being arranged on IAT ponds end.When pool water level reaches when process cycle starts most During low water level, water is strained in stopping.
(5) leave unused：The property of visual sewage sets a lay-up period during starting after being precipitated in IAT ponds to the next cycle, at this It can be stirred or be aerated as needed in period.Energy will be saved than the aeration under aerobic condition by stirring under anaerobic, The activity for keeping sludge is also advantageous simultaneously.In the device for the purpose of dephosphorization, the discharge of excess sludge usually exists At the beginning of idle process and precipitation process last progress.
The more conventional method of COD total amounts that DAT-IAT ponds remove is high, because IAT systems are run under unsteady state condition, experience Anaerobism, anoxic, aerobic stage, biology is considerably complicated in IAT ponds, and microbe species enrich, and can be metabolized by number of ways, make to have The degraded of machine thing is more complete.
The present invention puts into lipid-loweringing bacteria agent and drop protein fungus according to the characteristic of DAT-IAT sewage treatment process in DAT ponds Microbial inoculum, dead or aging thalline in degrading activity sludge is realized while degradation of organic substances, is not influenceing effluent quality In the case of, by the generating capacity for reaching reduction excess sludge to the process decrement of sludge.The input flocculation bacteria agent in IAT ponds, The volume for making sludge be more easy to after precipitation, easily dehydration and dehydration using the effect of flocculated bacteria is smaller.
The content of the invention
It is an object of the invention to provide a kind of complex micro organism fungicide for mud decrement and preparation method thereof, with solution The problem of being proposed in certainly above-mentioned background technology.
To achieve the above object, the present invention provides following technical scheme：A kind of composite microbial bacteria for mud decrement Agent, the complex micro organism fungicide contain：Flocculate bacteria agent, drop albumen bacteria agent and lipid-loweringing bacteria agent.
Wherein, flocculation bacteria agent includes beef extract, peptone, sodium chloride, starch, potassium dihydrogen phosphate, ammonium chloride, sulfuric acid Magnesium, ferrous sulfate and flocculated bacteria strain.
Wherein, drop albumen bacteria agent includes beef extract, peptone, sodium chloride, glucose, sodium dihydrogen phosphate, ammonium chloride, egg White degradation bacteria strain.
Wherein, lipid-loweringing bacteria agent includes beef extract, peptone, sodium chloride, glycerine, potassium dihydrogen phosphate, ammonium chloride, lipid-loweringing Bacterium strain.
Wherein, complex micro organism fungicide is applied to the DAT-IAT process sections of sewage disposal, and its specific method is to exist respectively Complex micro organism fungicide is put into DAT ponds and IAT ponds, drop albumen microbial inoculum and lipid-loweringing microbial inoculum are added in DAT ponds.By flocculated bacteria Agent is added in IAT ponds.20% sludge in IAT ponds is taken out and is back to after carrying out ultrasonication in DAT ponds.
Wherein, the quality of albumen microbial inoculum, the input amount of lipid-loweringing microbial inoculum and flocculation each microbial inoculum of microbial inoculum and pending mud drops Volume ratio is 0.03%:1.
Wherein, the preparation method of complex micro organism fungicide comprises the steps：
(1) preparation of zymotic fluid：It is enterprising in seed culture medium that protein fungus, lipid-loweringing bacterium and flocculated bacteria difference inclined plane inoculating will drop Row activation；35-37 DEG C of cultivation temperature is kept, rotating speed 200-220rPm is cultivated 1-2 days；
Fermented and cultured；By each strain by 1%-2% inoculum concentrations access fermentation medium, 35-37 DEG C of cultivation temperature is kept, Rotating speed 200-22Orpm, incubated 8-12h, zymotic fluid concentration OD600Reach 0.8-1, obtain mixed fermentation liquid.
Wherein, the composition of seed culture medium:Beef extract 0.5%, sodium chloride 0.5%, beef extract 1%, surplus are distilled water, PH adjustable ranges are 7.2；Fermentation medium is identical with the composition of seed culture medium.
(2) microbial inoculum is prepared；By mixed fermentation liquid 8000rpm centrifugations 8min obtained by step (1), protective agent is added；Per 10mg Bacterium mud, it is added in 30ml 0.2g/ml skimmed milk power, 6ml 1g/ml sucrose solution, 2ml is added after being sufficiently mixed uniformly 0.3g/ml Pidolidones sodium solution, 2ml 0.2g/ml vitamin c solutions；It is sufficiently mixed uniformly using mixer, is protected adding The bacterium mud of shield agent is stayed overnight in -80 DEG C of refrigerator pre-freezes, is placed into vacuum freeze drier and is dried, obtains microbial inoculum.
Compared with prior art, the beneficial effects of the invention are as follows：Spy of the invention according to DAT-IAT sewage treatment process Property, lipid-loweringing bacteria agent and drop albumen bacteria agent are put into DAT ponds, is realized while degradation of organic substances in degrading activity sludge Dead or aging thalline, it is remaining dirty by reaching reduction to the process decrement of sludge in the case where not influenceing effluent quality The generating capacity of mud.The input flocculation bacteria agent in IAT ponds, sludge is more easy to precipitation using the effect of flocculated bacteria, be easily dehydrated and be de- Volume after water is smaller.DAT ponds are back to after partial sludge in IAT ponds is carried out into ultrasonication, are made most of thin in sludge Bacterium is cracked, and intracellular organic matter is discharged into water, and is utilized for microorganism in DAT ponds, so as to reduce sludge yield.This method can make Mud decrement rate reaches 30%-70% in sewage disposal process, reduces the integrated operation cost of sewage treatment plant, has important Economy, environment and social effect.
Fig. 1：For the fundamental diagram of the present invention.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation Example only part of the embodiment of the present invention, rather than whole embodiments.It is common based on the embodiment in the present invention, this area The every other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects Enclose.
Embodiment 1
First, biomaterial and its source
Complex micro organism fungicide for mud decrement contains：Flocculate bacteria agent, drop albumen bacteria agent and lipid-loweringing bacteria agent.
Flocculation bacteria agent includes beef extract, peptone, sodium chloride, starch, potassium dihydrogen phosphate, ammonium chloride, magnesium sulfate, sulfuric acid Ferrous and flocculated bacteria strain.
Drop albumen bacteria agent includes beef extract, peptone, sodium chloride, glucose, sodium dihydrogen phosphate, ammonium chloride, albumen drop Solve bacterium strain.
Lipid-loweringing bacteria agent includes beef extract, peptone, sodium chloride, glycerine, potassium dihydrogen phosphate, ammonium chloride, lipid-loweringing bacterium bacterium Kind
These three strains are all conventional bacterial classifications, can from China General Microbiological culture presevation administrative center (CGMCC) or American Type Culture Collecti (ATCC) buys.
2nd, the preparation method of microbial bacterial agent comprises the steps：
(1) preparation of zymotic fluid：It is enterprising in seed culture medium that protein fungus, lipid-loweringing bacterium and flocculated bacteria difference inclined plane inoculating will drop Row activation；35-37 DEG C of cultivation temperature is kept, rotating speed 200-220rPm is cultivated 1-2 days.
Fermented and cultured；By each strain by 1%-2% inoculum concentrations access fermentation medium, 35-37 DEG C of cultivation temperature is kept, Rotating speed 200-22Orpm, incubated 8-12h, zymotic fluid concentration OD600Reach 0.8-1, obtain mixed fermentation liquid.
Wherein, the composition of seed culture medium:Beef extract 0.5%, sodium chloride 0.5%, beef extract 1%, surplus are distilled water, PH adjustable ranges are 7.2；Fermentation medium is identical with the formula of seed culture medium.
(2) microbial inoculum is prepared：By mixed fermentation liquid 8000rpm centrifugations 8min obtained by step (1), protective agent is added.Per 10mg Bacterium mud, it is added in 30ml 0.2g/ml skimmed milk power, 6ml 1g/ml sucrose solution, 2ml is added after being sufficiently mixed uniformly 0.3g/ml Pidolidones sodium solution, 2ml 0.2g/ml vitamin c solutions；It is sufficiently mixed uniformly using mixer, is protected adding The bacterium mud of shield agent is stayed overnight in -80 DEG C of refrigerator pre-freezes, is placed into vacuum freeze drier and is dried, obtains microbial inoculum.
3rd, complex micro organism fungicide is applied to the DAT-IAT process sections of sewage disposal.
Its specific method is puts into complex micro organism fungicide in DAT ponds and IAT ponds respectively, by described drop albumen microbial inoculum It is added to lipid-loweringing microbial inoculum in DAT ponds；Described flocculation microbial inoculum is added in IAT ponds；And 20% sludge in IAT ponds is taken out DAT ponds are back to after carrying out ultrasonication.
The mass volume ratio of albumen microbial inoculum, the input amount of lipid-loweringing microbial inoculum and flocculation each microbial inoculum of microbial inoculum and pending mud drops For 0.03%:1.Accurate adjustable means will be had by adding.Dosage is small, then flocculating effect is poor；And dosage is big, then on the one hand meeting Cause to waste, on the other hand can form anti-colloid, can not be separated with medium.
Embodiment 2
For the preparation method of the complex micro organism fungicide of mud decrement, comprise the steps：
(1) preparation of zymotic fluid：It is enterprising in seed culture medium that protein fungus, lipid-loweringing bacterium and flocculated bacteria difference inclined plane inoculating will drop Row activation；35 DEG C of cultivation temperature is kept, rotating speed 200-220rPm is cultivated 2 days.
Fermented and cultured：By each strain by 1%-2% inoculum concentrations access fermentation medium, 35-37 DEG C of cultivation temperature is kept, Rotating speed 200-22Orpm, incubated 8-12h, zymotic fluid concentration OD600Reach 0.8-1, obtain mixed fermentation liquid.
(2) microbial inoculum is prepared：By mixed fermentation liquid 8000rpm centrifugations 8min obtained by step (1), protective agent is added.Per 10mg Bacterium mud, it is added in 30ml 0.2g/ml skimmed milk power, 6ml 1g/ml sucrose solution, 2ml is added after being sufficiently mixed uniformly 0.3g/ml Pidolidones sodium solution, 2ml 0.2g/ml vitamin c solutions；It is sufficiently mixed uniformly using mixer, is protected adding The bacterium mud of shield agent is stayed overnight in -80 DEG C of refrigerator pre-freezes, is placed into vacuum freeze drier and is dried, obtains microbial inoculum.
Embodiment 3
Certain sewage plant, the inflow of sewage is 10 ton days, and sewage enters DAT ponds, continuously exposed while continuum micromeehanics Gas.The mechanism of organics removal and operation are identical with continuous stream activated sludge process.The Part II of reaction process occurs in IAT Pond, the sewage through the preliminary biological treatment in DAT ponds continuously enter IAT.The aeration for carrying out certain time is set to reach by technique The purpose of oxygen.Added in DAT ponds into drop albumen microbial inoculum and lipid-loweringing microbial inoculum, it is to add 0.03g's in 1L sludge to control addition Albumen microbial inoculum and lipid-loweringing microbial inoculum drop.The floc sludge sewage mixture of DAT ponds outlet enters IAT reaction tanks, as IAT reaction tanks The part nutriment of biochemical reaction.Added in IAT reaction tanks into flocculation bacteria agent, it is to be added in 1L sludge to control addition 0.03g flocculation bacteria agent.After stopping aeration when IAT ponds, active pollution index starts gravitational settling and mud-water separation, heavy The sludge to form sediment is back in DAT ponds after carrying out ultrasonication by 20%wt amounts, and another part excludes through delivery port.Activity Sludge sludge concentration after sample concentration reaches 60g/L, moisture content 85.97%, the analog loop under the conditions of 35 DEG C, 80r shaking tables Border condition carries out processing 72h, and mud decrement reaches 48.33%, and moisture content is down to 50.32%.
The present invention puts into lipid-loweringing bacteria agent and drop protein fungus according to the characteristic of DAT-IAT sewage treatment process in DAT ponds Microbial inoculum, dead or aging thalline in degrading activity sludge is realized while degradation of organic substances, is not influenceing effluent quality In the case of, by the generating capacity for reaching reduction excess sludge to the process decrement of sludge.The input flocculation bacteria agent in IAT ponds, The volume for making sludge be more easy to after precipitation, easily dehydration and dehydration using the effect of flocculated bacteria is smaller.Partial sludge in IAT ponds is carried out DAT ponds are back to after ultrasonication, make most of bacteria lysis in sludge, intracellular organic matter is discharged into water, and for DAT ponds Middle microorganism utilizes, so as to reduce sludge yield.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling Change is included in the present invention.

Claims (7)

  1. A kind of 1. complex micro organism fungicide for mud decrement, it is characterised in that：The complex micro organism fungicide contains：Flocculated bacteria Microbial inoculum, drop albumen bacteria agent and lipid-loweringing bacteria agent.
  2. A kind of 2. complex micro organism fungicide for mud decrement according to claim 1, it is characterised in that：Described wadding Solidifying bacteria agent includes beef extract, peptone, sodium chloride, starch, potassium dihydrogen phosphate, ammonium chloride, magnesium sulfate, ferrous sulfate and wadding Solidifying bacterium strain.
  3. A kind of 3. complex micro organism fungicide for mud decrement according to claim 1, it is characterised in that：Described drop Albumen bacteria agent includes beef extract, peptone, sodium chloride, glucose, sodium dihydrogen phosphate, ammonium chloride, protein degradation bacterium strain.
  4. A kind of 4. complex micro organism fungicide for mud decrement according to claim 1, it is characterised in that：Described drop Fat bacteria agent includes beef extract, peptone, sodium chloride, glycerine, potassium dihydrogen phosphate, ammonium chloride, lipid-loweringing bacterium strain.
  5. A kind of 5. complex micro organism fungicide for mud decrement according to claim 1, it is characterised in that：Described answers The DAT-IAT process sections that microbial bacterial agent is applied to sewage disposal are closed, put into complex microorganism in DAT ponds and IAT ponds respectively Microbial inoculum；Its specific method is that described drop albumen microbial inoculum and lipid-loweringing microbial inoculum are added in DAT ponds；Described flocculation microbial inoculum is thrown It is added in IAT ponds；And DAT ponds will be back to after 20% sludge taking-up progress ultrasonication in IAT ponds.
  6. A kind of 6. complex micro organism fungicide for mud decrement according to claim 1, it is characterised in that：The drop egg The mass volume ratio of white microbial inoculum, the input amount of lipid-loweringing microbial inoculum and each microbial inoculum of microbial inoculum that flocculates and pending mud is 0.03%:1.
  7. 7. a kind of complex micro organism fungicide for mud decrement according to claim 1, it is characterised in that described answers The preparation method for closing microbial bacterial agent comprises the steps：
    (1) preparation of zymotic fluid：Will drop protein fungus, lipid-loweringing bacterium and flocculated bacteria respectively inclined plane inoculating in being lived on seed culture medium Change；35-37 DEG C of cultivation temperature is kept, rotating speed 200-220rPm is cultivated 1-2 days；
    Fermented and cultured；By each strain by 1%-2% inoculum concentrations access fermentation medium, 35-37 DEG C of cultivation temperature, rotating speed are kept 200-22Orpm, incubated 8-12h, zymotic fluid concentration OD600Reach 0.8-1, obtain mixed fermentation liquid；
    Wherein, the composition of seed culture medium:Beef extract 0.5%, sodium chloride 0.5%, beef extract 1%, surplus are distilled water, and pH is adjusted Adjusting range is 7.2；Fermentation medium is identical with the composition of seed culture medium；
    (2) microbial inoculum is prepared；By mixed fermentation liquid 8000rpm centrifugations 8min obtained by step (1), protective agent is added；Per 10mg bacterium muds, It is added in 30ml 0.2g/ml skimmed milk power, 6ml 1g/ml sucrose solution, 2ml is added after being sufficiently mixed uniformly 0.3g/ml Pidolidones sodium solution, 2ml 0.2g/ml vitamin c solutions；It is sufficiently mixed uniformly using mixer, is protected adding The bacterium mud of shield agent is stayed overnight in -80 DEG C of refrigerator pre-freezes, is placed into vacuum freeze drier and is dried, obtains microbial inoculum.
CN201610688116.8A 2016-08-19 2016-08-19 A kind of complex micro organism fungicide for mud decrement and preparation method thereof Pending CN107759042A (en)

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Application publication date: 20180306