CN107177507A - A kind of method for cultivating high-density suspended floading condition helotism body - Google Patents
A kind of method for cultivating high-density suspended floading condition helotism body Download PDFInfo
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- CN107177507A CN107177507A CN201710614121.9A CN201710614121A CN107177507A CN 107177507 A CN107177507 A CN 107177507A CN 201710614121 A CN201710614121 A CN 201710614121A CN 107177507 A CN107177507 A CN 107177507A
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- helotism
- membrane bioreactor
- algae
- sludge
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention provides a kind of method for cultivating high-density suspended floading condition helotism body, comprises the following steps：1) choose in membrane bioreactor, membrane bioreactor provided with the filter for preventing bacterium algae from flowing out；2) seed sludge and algae kind into membrane bioreactor；3) sewage containing organic matter and nitrogen phosphorus is continuously added into membrane bioreactor, sludge and algae kind are mixed in water body, form suspended state helotism body, the hydraulic detention time of sewage in the reactor is 4~48h, biological more than the 4 days residence time, obtain high-density suspended floading condition helotism body.The high-density suspended floading condition helotism bulk concentration that the present invention is cultivated exceeds well over all documents and materials reported, the bioreactor using high-density suspended floading condition helotism body as core is turned out, the efficiency of its conversion pollutant exceedes all suspended state bacterium algae reactors reported.
Description
Technical field
The present invention relates to field of biological sewage treatment, more particularly to a kind of culture high-density suspended floading condition helotism body
Method.
Background technology
The biologic treating technique of current sewage relies primarily on conversion capability of the bacterium to pollutant.Using at immobilization cell
In terms of managing sewage, low concentration helotism system is present in the underloads such as oxidation pond, big hydraulic detention time for a long time
Technique in, mainly for the treatment of the water outlet of activated sludge process.At present, helotism system handles the efficiency of sanitary sewage still
So relatively low, the hydraulic detention time required for it shows as bacterium algae reactor needs 2~5 days.Bacterium algae reactor is more inefficient
Reason is biological concentration relatively low in reactor.Current suspended state syntaxial system concentration is concentrated between 0.3~0.6g/L, should
Concentration is well below the concentration reached in activated sludge process.Because algae and bacterium are easy to be lost in from conventional reactor, very
Big part research uses biomembrane process to improve the bacteria and algae amount in reactor.But largely used in biomembrance process
The propagation of filler meeting severe jamming light, and the microbes of biofilm surface play interception to bottom microorganism, this causes
The sewage treating efficiency of the helotism body built using biomembrane mode is difficult to improve.
The content of the invention
The shortcoming of prior art, high-density suspended floading condition bacterium is cultivated it is an object of the invention to provide one kind in view of the above
The method of algae homobium, the low problem of sewage treating efficiency for solving helotism body in the prior art.
In order to achieve the above objects and other related objects, the present invention provides a kind of culture high-density suspended floading condition helotism body
Method, comprise the following steps：
1) choose in membrane bioreactor, membrane bioreactor provided with the filter for preventing bacterium algae from flowing out；
2) seed sludge and algae kind into membrane bioreactor；
3) it is continuously added into membrane bioreactor containing organic matter, ammonia nitrogen and phosphatic sewage, sludge and algae kind
Mixed in water body, form suspended state helotism body, the hydraulic detention time of sewage in the reactor is 4~48h, and biology stops
Stay the time more than 4 days, obtain helotism body.
Further, step 2) in, the sludge is selected from the mud material rich in microorganism.
Preferably, step 2) in, the sludge is selected from river, the mud of lake-bottom, or sewage biological treatment facility
In sludge.
Sludge can use the sludge in conventional sewage plants, or mud, soil in the nature rich in microorganism
The media such as earth.As long as containing a certain amount of microorganism in sludge, after inoculation, you can amount reproduction, play in removal sewage
The effect of organic matter.
Further, step 2) in, the algae kind is selected from least one of blue-green algae, green alga, diatom.It can use single
Algae kind, such as blue-green algae, green alga etc.；Or use in the mixed algae grown naturally in artificial-strengthening treatment facility, such as sewage treatment plant
Algae on second pond wall.The algae kind is preferably mixed algae.
Further, step 2) in, the inoculum density of sludge and algae kind is respectively 0.5-5g/L.
Further, step 3) in, organic concentration is 50-10000mg/L in sewage, and ammonia nitrogen concentration is 10-500mg/
L, phosphate concn is 5-100mg/L.
Wherein, organic matter is specifically as follows acetate etc..
Further, the helotism body into membrane bioreactor provides 200~500 μm of ol/ (m2S) illumination is strong
Degree.
Further, oxygen concentration is 0~0.5mg/L in membrane bioreactor.
Further, oxygen concentration is 0~0.05mg/L in membrane bioreactor.
Further, the liquid in membrane bioreactor is stirred, mixing speed is 1~200rpm.
Further, the liquid in membrane bioreactor is stirred, mixing speed is 10~200rpm.
Further, step 3) in, the sewage temperature into membrane bioreactor is 15~30 DEG C, and pH is 6.5-7.5.On
State temperature and the growth of pH scopes suitable micro-organisms.
Further, step 3) in, the biological residence time is 4-30 days.The biological residence time refers to that bacterium algae is total in reactor
The total amount of raw body and the ratio per daily output.
Further, by step 3) ongoing operation 2-4 months, until the concentration for realizing helotism body in reactor is 3-
6g/L.If the excessive concentration of helotism body, bacterium can be caused too many, algae very little, in turn results in the coordinative role of the two and lost.
Inoculation is simply carried out in the early stage, after the helotism bulk concentration in reactor reaches 3-6g/L, it is only necessary to maintenance, no
Need constantly inoculation.
As described above, a kind of method of culture high-density suspended floading condition helotism body of the present invention, with following beneficial effect
Really：The high-density suspended floading condition helotism bulk concentration that the present invention is cultivated exceeds well over all documents and materials reported, turn out with
High-density suspended floading condition helotism body is the bioreactor of core, the efficiency of its conversion pollutant exceed it is all reported it is outstanding
Floading condition bacterium algae reactor.
Brief description of the drawings
Fig. 1 is shown as the culture high-density suspended floading condition helotism reactor according structural representation of use of the embodiment of the present invention
Figure.
Fig. 2 is shown as biological concentration growth curve figure in the reactor of the embodiment of the present invention.
Piece mark explanation
1-intake pump
2-reactor
3-light source
4-helotism body
5-filter
6-agitating device
7-go out water pump
Embodiment
Illustrate embodiments of the present invention below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through specific realities different in addition
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints with application, without departing from
Various modifications or alterations are carried out under the spirit of the present invention.
Embodiment 1
The present embodiment is using 2L beaker as reactor, and structure of reactor is as shown in figure 1, reactor 2 in the present embodiment
For membrane bioreactor, from filter that silks is made is split as filter 5, the delivery port of reactor 2 is installed on, was played
The effect of water but only microorganism.It is inoculated with into reactor 2 in conventional sludge and algae kind, the present embodiment inoculation municipal sewage plant
The inoculum density of mixed algae on Aerobic Pond activated sludge and precipitation pool wall, sludge and algae kind is 2g/L.Water in reactor 2
Temperature is 25 DEG C, and pH is between 6.5~7.5, reactor.Operations described below is performed to reactor：Using the continuation of intake pump 1
Be pumped into the sewage containing organic matter and nitrogen phosphorus into reactor 2, sewage in the reactor after treatment, through filter 5
Discharged after filtering, filter 5 can play prevention helotism body and be flowed out with water body, and outlet conduit is provided with out water pump 7, is
The discharge of water provides power.Acetate concentration 300mg COD/L, ammonia nitrogen 40mg/L, phosphate 15mg/L in water inlet.Reactor 2
Hydraulic detention time be 24 hours.Due to the presence of filter 5, reactor 2 will not outwards discharge micro- by filter 5
Biology, uses light intensity for 200 μm of ol/ (m2S) light source 3 provides illumination to reactor 2.The top of reactor 2 is in opening knot
Structure, when helotism bulk concentration is too high, can be discharged excessive helotism body from upper opening by the opening so that
Helotism body maintains in reasonable concentration, sewage disposal process and does not need artificial aeration, is produced using algae photosynthesis
Oxygen as bacterium oxygen sources, you can meet the oxygen demand of bacterium, measure the Dissolved Oxygen in Water concentration of reactor 20
Between~0.05mg/L.Water body is stirred using agitating device 6 in reactor 2, rotating speed 100rpm.According to the operating method
Ongoing operation about 3 months, until the concentration of helotism body 4 in reactor 2 reaches 4g/L or so.Reactor to influent COD and
The removal efficiency of ammonia nitrogen is 100%, and phosphate removal efficiency is 50%.Biological concentration versus time curve is such as in reactor
Shown in Fig. 2.
In summary, the method for a kind of culture high-density suspended floading condition helotism body of the invention, with following beneficial effect
Really：The high-density suspended floading condition helotism bulk concentration that the present invention is cultivated exceeds well over all documents and materials reported；By the present invention
Turn out the bioreactor using high-density suspended floading condition helotism body as core, the efficiency of its conversion pollutant exceed it is all
The suspended state bacterium algae reactor of report.For example, use hydraulic detention time be 24 hours build reactor to ammonia nitrogen, organic matter
Conversion capability be 100%.The suspended state bacterium algae reactor reported will reach that this efficiency needs the hydraulic retention of 3~6 days
Time.The reactor configuration that the present invention is used is different from prior art, and the present invention is only with a reactor rather than using one
Row reactor, what the present invention was cultivated is the helotism body of suspended state helotism body rather than attached state, nor being in granular form
The helotism body of state of aggregation, does not use aeration to operate, it is not necessary to add carbon dioxide and ozone in phototropic reaction device, be not required to yet
Want follow-up flocculation sedimentation tank, it is not required that preposition two bioreactors are pre-processed, what the present invention was cultivated is helotism body
Rather than single algae, the present invention need not individually be passed through oxygen, energy consumption be saved, while being realized in single reactor has
Machine thing, ammonia nitrogen, phosphatic removal, without carrying out multistep treatment, effectively improve sewage treating efficiency.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of method for cultivating high-density suspended floading condition helotism body, it is characterised in that comprise the following steps：
1) choose in membrane bioreactor, membrane bioreactor provided with the filter for preventing bacterium algae from flowing out；
2) seed sludge and algae kind into membrane bioreactor；
3) it is continuously added into membrane bioreactor containing organic matter, ammonia nitrogen and phosphatic sewage, sludge and algae kind are in water
Mixed in body, form suspended state helotism body, the hydraulic detention time of sewage in the reactor is 4~48h, when biology is stopped
Between more than 4 days, obtain high-density suspended floading condition helotism body.
2. according to the method described in claim 1, it is characterised in that：Step 2) in, the sludge is selected from the mud rich in microorganism
Material；Preferably, the sludge is selected from river, the mud of lake-bottom, or the sludge in sewage biological treatment facility.
3. according to the method described in claim 1, it is characterised in that：Step 2) in, the algae kind is selected from blue-green algae, green alga, diatom
At least one of.
4. according to the method described in claim 1, it is characterised in that：Step 2) in, the inoculum density of sludge and algae kind is respectively
0.5-5g/L。
5. according to the method described in claim 1, it is characterised in that：Step 3) in, organic concentration is 50- in sewage
10000mg/L, ammonia nitrogen concentration is 10-500mg/L, and phosphate concn is 5-100mg/L.
6. according to the method described in claim 1, it is characterised in that：Helotism body into membrane bioreactor provides 200
~500 μm of ol/ (m2S) intensity of illumination.
7. according to the method described in claim 1, it is characterised in that：Liquid in membrane bioreactor is stirred, stirred
Speed is 1~200rpm, it is preferable that mixing speed is 10~200rpm.
8. according to the method described in claim 1, it is characterised in that：Step 3) in, into the sewage temperature of membrane bioreactor
For 15~30 DEG C, pH is 6.5-7.5.
9. according to the method described in claim 1, it is characterised in that：Step 3) in, the biological residence time is 4-30 days.
10. according to the method described in claim 1, it is characterised in that：By step 3) ongoing operation 2-4 months, it is anti-until realizing
The concentration for answering helotism body in device is 3-6g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710614121.9A CN107177507A (en) | 2017-07-25 | 2017-07-25 | A kind of method for cultivating high-density suspended floading condition helotism body |
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| CN201710614121.9A CN107177507A (en) | 2017-07-25 | 2017-07-25 | A kind of method for cultivating high-density suspended floading condition helotism body |
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| CN107177507A true CN107177507A (en) | 2017-09-19 |
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| CN201710614121.9A Pending CN107177507A (en) | 2017-07-25 | 2017-07-25 | A kind of method for cultivating high-density suspended floading condition helotism body |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623099A (en) * | 2018-06-01 | 2018-10-09 | 浙江省农业科学院 | A kind of method and device of the aquaculture wastewater of bacteria-algae synergism degradation high concentration N content |
| CN110436614A (en) * | 2019-08-02 | 2019-11-12 | 大连理工大学 | It is a kind of based on microalgae/activated sludge water treatment technology |
| CN111661930A (en) * | 2020-06-08 | 2020-09-15 | 浙江工业大学 | Method for repairing arsenic pollution in high-saline-alkali water body by using calcium ion enhanced blue algae-phycomycetes biomembrane |
| CN114349165A (en) * | 2022-01-13 | 2022-04-15 | 中国水产科学研究院黄海水产研究所 | Construction method and application of algae-bacterium symbiotic denitrification biofilter |
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| CN102211834A (en) * | 2010-04-08 | 2011-10-12 | 何少林 | Light film symbiosis reaction device |
| US20120024780A1 (en) * | 2010-06-21 | 2012-02-02 | Herman Carr | Water treatment plant for combined biomass and biogas production |
| CN102372392A (en) * | 2010-08-16 | 2012-03-14 | 何少林 | Built-in membrane symbiosis reactor |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623099A (en) * | 2018-06-01 | 2018-10-09 | 浙江省农业科学院 | A kind of method and device of the aquaculture wastewater of bacteria-algae synergism degradation high concentration N content |
| CN108623099B (en) * | 2018-06-01 | 2021-04-16 | 浙江省农业科学院 | Method and device for degrading high-concentration nitrogen-content aquaculture sewage through cooperation of bacteria and algae |
| CN110436614A (en) * | 2019-08-02 | 2019-11-12 | 大连理工大学 | It is a kind of based on microalgae/activated sludge water treatment technology |
| CN111661930A (en) * | 2020-06-08 | 2020-09-15 | 浙江工业大学 | Method for repairing arsenic pollution in high-saline-alkali water body by using calcium ion enhanced blue algae-phycomycetes biomembrane |
| CN114349165A (en) * | 2022-01-13 | 2022-04-15 | 中国水产科学研究院黄海水产研究所 | Construction method and application of algae-bacterium symbiotic denitrification biofilter |
| CN114349165B (en) * | 2022-01-13 | 2022-11-22 | 中国水产科学研究院黄海水产研究所 | Construction method and application of algae-bacterium symbiotic denitrification biofilter |
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Application publication date: 20170919 |
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