CN104560823B - Can degrade acetonitrile Shewanella putrefaciens and its application - Google Patents
Can degrade acetonitrile Shewanella putrefaciens and its application Download PDFInfo
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- CN104560823B CN104560823B CN201410855757.9A CN201410855757A CN104560823B CN 104560823 B CN104560823 B CN 104560823B CN 201410855757 A CN201410855757 A CN 201410855757A CN 104560823 B CN104560823 B CN 104560823B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
- C02F2103/365—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds from petrochemical industry (e.g. refineries)
Abstract
Shewanella putrefaciens and its application the invention provides a kind of energy efficient degradation acetonitrile, the Shewanella putrefaciens (Shewanella putrefaciens) of the acetonitrile that can degrade of the invention, its deposit number is CGMCC No.10148.The bacterial strain can be used to process the sewage of petroleum chemical enterprise containing low-concentration acetonitrile and river, lake ripple, seawater for being polluted by petroleum chemical enterprise's sewage etc., with to petroleum chemical enterprise sewage strong adaptability, biological stability is good the features such as, can effectively remove the acetonitrile of low concentration in petrifaction sewage.
Description
Technical field
The present invention is Shewanella putrefaciens and its application on a kind of energy efficient degradation acetonitrile, and in particular to one kind can
The Black Liquor with Efficient Bacteria and its related application of acetonitrile, are related to petrifaction sewage process field in fast degradation petrifaction sewage.
Background technology
With the development and the implementation of Integrated Refinery And Petrochemical of petroleum chemical industry, petroleum chemical enterprise's technique becomes increasingly complex, and produces
Kind class is more and more, and the thing followed is that the organic matter for generating large amount of complex is entered into sewage disposal system.It is original
Sewage disposal system can not completely be adapted in face of difficult degradation, highly toxic organic matter and cause to occur that water outlet is up to standard, waterpower is stopped
The problems such as staying time lengthening.
Acetonitrile also known as acetonitrile, are a kind of organic compounds containing cyanogen, are widely used in petrochemical industry, printing and dyeing, pharmacy, conjunction
Into fields such as fibers.In often thering is a certain amount of acetonitrile to enter waste water during acetonitrile associated production or its use, particularly closely
Increasing sharply with acetonitrile yield and consumption over year, the discharge capacity containing acetonitrile sewage also increases year by year.Acetonitrile is by wedging, suction
Enter and skin absorption can be entered into organism, extremely toxic substance hydrogen cyanide and acetaldehyde are decomposed into vivo, when without
The sewage waste liquor containing acetonitrile for the treatment of causes serious ecology into that can cause the large quantities of death of the aquatiles such as fish after natural water area
Environmental disruption, endangers human and livestock health.Therefore, national bioengineering class pharmaceuticals industry pollution discharge standard (GB 21907-
2008) in clear stipulaties enterprise sewage effluent ethane nitrile content not above 3.0mg/L.
Current existing acetonitrile treatment method is mainly chemical method, for example：Ozone or photocatalytic oxidation, but its is anti-
Answer condition very harsh, construction costs is also very high, and acetonitrile is readily volatilized, so generally using few.So to acetonitrile
Treatment research it is more and more.But for the treatment of acetonitrile sewage, studies in China is seldom, biological according to some external researchs
Method has occupied obvious advantage, mainly uses the metabolism of microorganism to be particularly the nitrile hydratase produced in microbial metabolism
In the presence of amidase, acetonitrile is degraded to organic acid and ammonia.
Bioanalysis degraded acetonitrile to do is to the screening of degradation bacteria first, only with the microorganism of efficient degradation acetonitrile,
Optimal treatment effect can just be obtained.The microorganism of some degradable acetonitriles, such as withered grass gemma CBS489- have been filtered out at present
74th, candida guilliermondii CCT7202, Klebsiella oxytoca, pseudomonas putida, Rhodococcus sp A10, Rhodococcus sp BL1, Rhodococcus sp
PA-34 etc., these bacterium can be grown by the use of phthalic acid ester or acetonitrile as unique carbon source and nitrogen source.Separately, Li Yue et al.
One plant of purplish red coccus (Rhodococcus rhodochrous) is filtered out to concentration for the acetonitrile degradation rate of 800mg/L is
95.87% (" identification of one plant of acetonitrile degradation bacteria and its biological characteristics ", Li Yue et al.,《Microorganism journal》, 2012 02
Phase).However, the studies above is to acetonitrile single compound and high concentration acetonitrile simulated wastewater does Degrading experiment, to actual dirt
Research in water in terms of the degradation capability and strain of acetonitrile and original sewage disposal system compatibility seldom, not yet forms complete
Can improve acetonitrile degradation capability in sewage, reach the technology of discharging standards.
The content of the invention
The main object of the present invention is to provide one plant being capable of acetonitrile and and traditional activated sludge process in efficient degradation sewage
There is the strain of good compatibility, can strengthen original sewage disposal system by bioanalysis to improve the removal of acetonitrile in sewage
Efficiency, reaches sewage drainage standard.
Application another object of the present invention is to provide the above-mentioned strain for being capable of efficient degradation acetonitrile.
Inventor has filtered out 1 plant from the activated sludge of certain petrochemical plant biologic process for treating sewage can efficiently drop
The bacterial strain of acetonitrile, ZLR3 is named as in the present invention in solution sewage.It is micro- that the ZLR3 bacterial strains are preserved in China on December 9th, 2014
Biological inoculum preservation administration committee's common micro-organisms center (address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese section
Institute of microbiology of institute), deposit number：CGMCC No.10148.
The bacterial strain ZLR3 that the present invention is filtered out, is identified as Shewanella putrefaciens (Shewanella
Putrefaciens), it stabilization can grow in 25 DEG C~40 DEG C of environment, and up to 3%, the pH scopes of adaptation exist the tolerance of salinity
Between 6~8, with wide degrees.Find that the bacterial strain being capable of efficient degradation second by substantial amounts of experiment and analysis
Nitrile, also has obvious effect for other persistent organic pollutants in petroleum chemical enterprise's sewage, can increase substantially petrochemical industry
The biodegradability of sewage.
So as on the basis of the above, present invention also offers the Shewanella putrefaciens CGMCC of the acetonitrile that can degrade
The biology pure culture of No.10148 bacterial strains.
For convenience of preservation and concrete operations application, present invention also offers a kind of Shewanella putrefaciens of the acetonitrile that can degrade
Bacteria preparation, containing the Shewanella putrefaciens bacterial strain of the acetonitrile that can degrade that deposit number is CGMCC No.10148 in the bacteria preparation,
The bacteria preparation is specifically as follows solid-state or liquid bacteria preparation.
Present invention also offers described Shewanella putrefaciens CGMCC No.10148 or described biology pure culture
Or application of the described bacteria preparation in acetonitrile of degrading.Specifically, can be by Shewanella putrefaciens CGMCC of the invention
No.10148 or described biology pure culture or described bacteria preparation are used to process the various water bodys containing acetonitrile, for example can be with
It is the water body polluted by acetonitrile of petrifaction sewage and/or other industry generations.These water bodys can also come to be polluted by acetonitrile
River, lake and/or ocean.
In view of the growth characteristics of Shewanella putrefaciens CGMCC No.10148 of the invention, using of the invention corrupt uncommon
During sewage containing acetonitrile of watt Salmonella CGMCC No.10148 or described biology pure culture or the treatment of described bacteria preparation, lead to
Can often implement under field conditions (factors)；If facing particular surroundings, can artificially be adjusted, the temperature of system for handling is maintained 25
DEG C~40 DEG C, below 3%, pH scopes are between 6~8 for salt content.
In addition, using Shewanella putrefaciens CGMCC No.10148 of the invention or described biology pure culture or
When described bacteria preparation treatment contains the sewage of acetonitrile, acetonitrile concentration has certain to the removal efficiency of COD and acetonitrile in system
Influence, acetonitrile concentration≤50mg/L in preferred system in the present invention.In a specific embodiment of the invention, work as ethane nitrile content
During for 40mg/L, CGMCC No.10148 are 53.9% to the clearance of COD of sewage, and the clearance to acetonitrile is 96.37%, is gone out
Water-acetonitrile concentration meets national standard.
On the other hand, present invention also offers it is a kind of process containing acetonitrile petrifaction sewage method, the method using
Airlift bioreactor is processed the petrifaction sewage containing acetonitrile, wherein, inoculation is active in airlift bioreactor
The Shewanella putrefaciens CGMCC No.10148 of sludge and the acetonitrile that can degrade of the present invention.Treatment of the invention contains acetonitrile
The utilization that it is critical only that the Shewanella putrefaciens CGMCC No.10148 of the method for petrifaction sewage, other works in the method
Skill is carried out in can refer to the prior art of art.Preferably, in the method for petrifaction sewage of the treatment containing acetonitrile of the invention, institute
The Shewanella putrefaciens CGMCC No.10148 of the acetonitrile that can degrade stated are previously prepared into seed liquor, then with activated sludge with 1:
3~3：1 volume ratio combined inoculation, inoculum concentration is preferably the 1/6~1/3 of airlift bioreactor basin's effective volume.
The present invention has advantages below：
(1) efficient bacteria preparation ZLR3 of the invention, with to the spy such as sewage disposal system strong adaptability, biological stability be good
Point, can be processed petrifaction sewage containing acetonitrile, be applicable to salinity and more big pH value range higher.
(2) the efficient bacteria preparation ZLR3 of acetonitrile improves activated Sludge System in using degraded petrifaction sewage of the invention
During to the disposal ability of acetonitrile sewage, be inoculated with 33% inoculum concentration (v/v), by the COD removal efficiency of activated Sludge System by
36.35% brings up to 48.09%.
(3) the biological reinforced gas-lifting types of efficient bacteria preparation ZLR3 of acetonitrile are biological in using degraded petrifaction sewage of the invention
When reactor is the petrifaction sewage of 20~50mg/L to process containing acetonitrile concentration, clearance goes out water-acetonitrile dense more than 98%
Degree is less than 1mg/L, well below discharging standards.
Brief description of the drawings
Degradation effect of the pure bacterium of Fig. 1 ZLR3 to acetonitrile.
Fig. 2 acetonitrile concentrations are to BOD5The influence of/COD.
Influence of Fig. 3 acetonitrile concentrations to COD clearances.
Fig. 4 acetonitrile concentrations are to NH3The influence of-N.
Influence of Fig. 5 acetonitrile concentrations to toxicity.
Influence of Fig. 6 acetonitrile concentrations to acetonitrile degradation rate.
The compatibility of Fig. 7 ZLR3 and activated sludge.
Fig. 8 processes the schematic device of petrifaction sewage containing acetonitrile.
Treatment effect of the biological reinforced airlift bioreactors of Fig. 9 ZLR3 to various concentrations acetonitrile sewage.
The removal effect of ammonia nitrogen when the biological reinforced airlift bioreactors of Figure 10 ZLR3 process acetonitrile sewage.
Microorganism for proprietary program preserves：
The treatment Black Liquor with Efficient Bacteria of petrifaction sewage containing acetonitrile ZLR3 of the invention
Preservation date：On December 9th, 2014；
Depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC)；
Depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica
Deposit number：CGMCC No.10148；
Classification And Nomenclature：Shewanella putrefaciens (Shewanella putrefaciens).
Specific embodiment
Treatment petrifaction sewage containing acetonitrile of the invention is further described below by specific embodiment and with reference to accompanying drawing
The characteristics of Black Liquor with Efficient Bacteria, and using the method and the technique effect having of bacterium treatment petroleum chemical enterprise containing acetonitrile sewage, these
Embodiment is not to be read as the limitation to the application protection domain.
The screening of embodiment 1, Black Liquor with Efficient Bacteria
The specific composition and content of minimal medium used are in the present embodiment：Sodium chloride 1g/L, ammonium nitrate 1.5g/L,
Potassium dihydrogen phosphate 0.5g/L, dipotassium hydrogen phosphate 0.5g/L, green vitriol 0.02g/L, magnesium sulfate 0.1g/L, pH=7.2,
Acetonitrile concentration in culture medium is specifically shown in each embodiment；Acetonitrile purity used is chromatographically pure, in solution state.
From petrochina Dalian Petrochemical Industry Company sewage workshop activated sludge method processing technique O ponds, outlet takes activated sludge, uses
The method that liquid enrichment culture method, dilution plate rubbing method are combined is carried out to the Black Liquor with Efficient Bacteria of acetonitrile in degraded petroleum chemical enterprise sewage
Isolation and purification.Comprise the following steps that：
(1) the above-mentioned minimal mediums of 100mL are added in 250mL conical flasks, add acetonitrile to make its concentration for 50mg/L,
Weigh 5mL activated sludge samples to be inoculated with, shaken cultivation is carried out in shaking table, condition is 30 DEG C, 190rpm；
After (2) 1 weeks, take during 5mL nutrient solutions are transferred to the fresh minimal mediums containing acetonitrile 100mg/L of 100mL and train
Support, condition of culture is constant；
(3) after cultivating 1 week again, 5mL nutrient solutions are taken and is transferred to the fresh trainings of the inorganic salts containing acetonitrile 200mg/L of 100mL
Culture in base is supported, condition of culture is constant, so repeat domestication 3 weeks, each time acetonitrile concentration gradient increases 100mg/L, extremely
Domestication terminates after 500mg/L；
(4) by the Sample Dilution after domestication to finite concentration, the solid inorganic salt culture containing 500mg/L acetonitriles is coated
In base, the composition and content of the solid medium are as follows：Sodium chloride 1g/L, ammonium nitrate 1.5g/L, potassium dihydrogen phosphate 0.5g/L, phosphorus
Sour hydrogen dipotassium 0.5g/L, green vitriol 0.02g/L, magnesium sulfate 0.1g/L, agar 20g/L, pH=7.2,30 DEG C constant temperature
Culture, obtains different bacterium colonies, chooses single bacterium and rules repeatedly the purebred bacterial strain for obtaining and can utilizing acetonitrile, and the single bacterium that will be obtained connect respectively
Plant in the petrochemical wastewater containing acetonitrile, the COD removal efficiency bacteriums after relatively more each bacterial strain is acted on are comprised the following steps that：
(1) with certain petrochemical plant waste water and solution that acetontrile acetonitrile concentration is 40mg/L；
(2) the acetonitrile degradation germ liquid that will just sift out takes 7mL and 5min is centrifuged under 1000r/min respectively, uses distilled water weight
Centrifugation is repeated after outstanding；
(3) thalline and 250mL petrifaction sewages that will be centrifuged are added in BOD test bottles, are put into 20 DEG C of constant incubators
Middle test b OD, reading after 5 days；
The COD value of water sample in BOD bottles is determined after (4) 5 days, is compared with raw water COD, calculate its COD clearance and B/C, with
Filter out the strain for subsequent experimental.
4 plants of acetonitrile efficient degrading bacterias can be filtered out according to data ratio, DLS2, ZLR3, DLF, DR are named as respectively,
Specific data are shown in Table 1.
The acetonitrile degradation bacteria evaluating data of table 1
As shown in Table 1, the degradation effect of ZLR3 is best in 4 plants of acetonitrile efficient degrading bacterias, and identified its is corrupt Shiva
Salmonella (Shewanella putrefaciens), the 16S rDNA sequences of the bacterial strain are as shown in SEQ ID No.1.
Bio-chemical characteristics display ZLR3 has sports type, and catalase positive, Starch Hydrolysis are negative, and grape is glycoxidative
Fermentation shows as oxidized form, and methyl red test is negative.Bacterial strain ZLR3 is identified as Shewanella putrefaciens (Shewanella
Putrefaciens), Shewanella putrefaciens (Shewanella putrefaciens) ZLR3 is also known as in the present invention.The bacterial strain
China Committee for Culture Collection of Microorganisms's common micro-organisms center (address is preserved on December 9th, 2014：Beijing
The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica), deposit number：CGMCC No.10148.
Degradation characteristic of the embodiment 2, ZLR3 to acetonitrile
The used sewage sample of each experiment and activated sludge sample are taken from Dalian petrifaction sewage treatment in the present embodiment
Workshop, adds acetonitrile to be configured to the acetonitrile sewage of various concentrations in sewage.
LB nutrient media componentses used and content are in the present embodiment：Dusty yeast 5g/L, peptone 10g/L, sodium chloride 10g/
L, pH=7.2, by experimental strain inoculation LB culture mediums after after culture 18h under the conditions of 35 DEG C, 120r/min, use as seed liquor
In following experiments in the present embodiment (the present embodiment and bacterium solution used by follow-up each embodiment are the seed liquor of each bacterium).
The experiment 1, degradation rate to acetonitrile
The seed liquor of bacterial strain ZLR3, DR, DLS3 to be screened in the inoculum concentration inoculation embodiment 1 of 5% (v/v) is different to containing
In the inorganic salts liquid culture medium of concentration acetonitrile, the specific composition and content of inorganic salts liquid culture medium are：Sodium chloride 1g/L, nitre
Sour ammonium 1.5g/L, potassium dihydrogen phosphate 0.5g/L, dipotassium hydrogen phosphate 0.5g/L, green vitriol 0.02g/L, magnesium sulfate
0.1g/L, pH=7.2, acetonitrile concentration are respectively 10mg/L, 20mg/L, 30mg/L, 40mg/L, 50mg/L, 30 DEG C, 190r/min
Shaking table culture 24h, with the acetonitrile concentration in gas Chromatographic Determination nutrient solution, calculates degradation rates of the ZLR3 to various concentrations acetonitrile.
Result is as shown in figure 1, when acetonitrile concentration is 10mg/L, 20mg/L, 30mg/L, ZLR3 is to acetonitrile degradation rate
100%.With the increase of acetonitrile concentration, ZLR3 has declined to the degradation rate of acetonitrile, and in 40mg/L, degradation rate is
99.32%, in 50mg/L, degradation rate is 98.37%, hence it is evident that better than bacterial strain DR and DLS3 that same batch is screened.
Experiment 2, acetonitrile concentration is to BOD5The influence of/COD
After adding ZLR3 seed liquors and activated sludge with the inoculum concentration of 5% (v/v), various concentrations acetonitrile sewage is determined
COD and BOD5。
Result is as shown in Fig. 2 with the increase of acetonitrile concentration, add the BOD of the sewage of ZLR3 and activated sludge5/ COD by
Gradually decline, illustrate that acetonitrile has certain toxicity to microorganism, but in the range of 0~50mg/L of acetonitrile concentration, add ZLR3 dirty
The BOD of water5/ COD is above adding the sewage of activated sludge, illustrates that ZLR3 can increase substantially the biodegradability of sewage.
Experiment 3, influence of the acetonitrile concentration to COD clearances
After adding ZLR3 seed liquors and activated sludge with the inoculum concentration of 5% (v/v), various concentrations acetonitrile sewage is determined
The clearance of COD.
Result is as shown in figure 3, with the increase of acetonitrile concentration, add the COD clearances of the sewage of ZLR3 and activated sludge
It has been risen that, the COD clearances for adding ZLR3 sewage are above adding the sewage of activated sludge, illustrate that ZLR3 can be increased substantially
The COD clearances of sewage.
Experiment 4, acetonitrile concentration is to NH3The influence of-N
After adding ZLR3 seed liquors and activated sludge with the inoculum concentration of 5% (v/v), various concentrations acetonitrile sewage is determined
NH3-N。
Result is as shown in figure 4, with the increase of acetonitrile concentration, add the NH of the sewage of ZLR33- N concentration keeps not substantially
Become, and add the NH of the sewage of activated sludge3- N concentration significantly rises.NH is produced after acetonitrile degraded3- N, illustrates that ZLR3 has
Digestion, makes the ammonia nitrogen level of sewage remain unchanged.
Experiment 5, influence of the acetonitrile concentration to toxicity
After adding ZLR3 seed liquors and activated sludge with the inoculum concentration of 5% (v/v), the poison of various concentrations acetonitrile sewage is determined
Property.
Result is as shown in figure 5, with the increase of acetonitrile concentration, add the toxicity of sewage of ZLR3 and activated sludge significantly
Degree rises, but the toxicity of the sewage of ZLR3 is added under each acetonitrile concentration than low, the explanation that adds the sewage of activated sludge
ZLR3 can substantially reduce the toxicity of sewage.
Experiment 6, influence of the acetonitrile concentration to acetonitrile degradation rate
After adding ZLR3 seed liquors and activated sludge with the inoculum concentration of 5% (v/v), second in various concentrations acetonitrile sewage is determined
The degradation efficiency of nitrile.
Result is as shown in fig. 6, with the increase of acetonitrile concentration, add the degraded of acetonitrile in the sewage of ZLR3 and activated sludge
Rate has decline, and acetonitrile concentration influences smaller to ZLR3, and influences larger to activated sludge, is 10~30mg/L in acetonitrile concentration
When, ZLR3 is 100% to the degradation rate of acetonitrile, and in higher concentration, degradation rate drops to 96.37%, 91.23%.
The compatibility of embodiment 3, ZLR3 and activated sludge
Activated sludge and ZLR3 seed liquors, DR bacterium seed liquors are respectively 1 with volume ratio:3、1:2、1:1、2:1、3:1 mixes
Close, then mixed liquor is inoculated with petrifaction sewage of the acetonitrile concentration as 50mg/L with the inoculum concentration of volume ratio 5%, the COD for determining sewage goes
Except rate.The preparation of seed liquor is with embodiment 2 in the present embodiment.
Result is as shown in fig. 7, by activated sludge and ZLR3 seed liquors with 1:To COD in ethane nitrile wastewater after the mixing of 3 ratios
Clearance highest, and overall COD clearances after ZLR3 mixes with activated sludge are above the effect after DR and activated sludge compatibility
Really, other bacterial strains that ZLR3 is screened in this research is substantially better than in improving the COD removal efficiency containing acetonitrile petrifaction sewage are illustrated.
Biological reinforced process pilot of the treatment containing acetonitrile petrifaction sewage of embodiment 4, ZLR3 is studied
The handling process of petrifaction sewage containing acetonitrile used in the present embodiment is airlift bioreactor, and reactor assembly shows
It is intended to as shown in figure 8, the dischargeable capacity of device is 4m3, device inoculation ZLR3 and activated sludge after 20d inflows reach 6m3/
d.The gas-water ratio of device is set as 10:1, when acetonitrile concentration is 20,30,40 and 50mg/L in investigation water inlet respectively, device is to second
The removal efficiency of nitrile, each acetonitrile concentration gradient operation 7d.
The ethane nitrile wastewater used in embodiment is certain petrochemical industry Love psychology treatment workshop A ponds water outlet addition acetonitrile, experiment dress
Put as shown in figure 8, A ponds mud mixture carries out mud-water separation through submersible sewage pump A1 input sludge settlings bucket 1, the sewage on top is in weight
Power effect is lower to flow into airlift bioreactor (BIOAX reactors) 2, and the sludge of precipitation bottom of the barrel sedimentation drains into sewage sludge storage bucket
3, ethane nitrile wastewater then returns A by entering sewage sludge storage bucket 3 after airlift bioreactor biological treatment by submersible sewage pump 31
Pond.The experimental provision is additionally provided with conventional liquid level gauge 11, ball valve 12, gas flowmeter 21 etc..Airlift bioreactor is gas
Lift-type internal circulation biological filter, its dissolved oxygen efficiency is high, can effectively reduce the volatilization of acetonitrile.During startup, gas-lifting type biological respinse
The inoculum concentration of device is 1/4, seed sludge 750L, ZLR3 bacterium solution 250L of basin's effective volume, and adds nutrient solution, then enters acetonitrile and gives up
Water to the page reaches delivery port, and vexed exposure starts continuum micromeehanics after 2 days, flow is 2m3/ d, temperature control is in 25~30 DEG C, air water
Than being controlled to 5:Inflow reaches 6m after 1,20d3/d。
Result as shown in figure 9, be respectively 20 in acetonitrile concentration of intaking, 30,40mg/L when, acetonitrile concentration is below in water outlet
0.1mg/L, clearance more than 99.5%；When water inlet acetonitrile concentration is 50mg/L, some floatings of acetonitrile concentration in water outlet, but
Below 1mg/L, meets discharging standards.Acetonitrile can produce amino during microbial degradation, so ammonia nitrogen is dense in water outlet
Degree is also the index investigated, and as a result as shown in Figure 10, ammonia nitrogen concentration is in below 0.5mg/L in water outlet.Pilot experiment is reflected
The biological reinforced airlift bioreactors of ZLR3 have good treatment effect to petrifaction sewage containing acetonitrile.
Claims (8)
1. a kind of Shewanella putrefaciens (Shewanella putrefaciens) of acetonitrile that can degrade, its deposit number is CGMCC
No.10148,16S rDNA sequences are as shown in SEQ ID No.1.
2. can be degraded described in claim 1 acetonitrile Shewanella putrefaciens biology pure culture.
3. a kind of Shewanella putrefaciens bacteria preparation of the acetonitrile that can degrade, contains deposit number for CGMCC in the bacteria preparation
The Shewanella putrefaciens bacterial strain of the acetonitrile that can degrade of No.10148, the 16S rDNA sequences of the bacterial strain as shown in SEQ ID No.1,
And the bacteria preparation is solid-state or liquid bacteria preparation.
4. biology pure culture described in the Shewanella putrefaciens or claim 2 described in claim 1 or claim 3 institute
Application in acetonitrile of the bacteria preparation stated in sewage of degrading, wherein, the temperature of system for handling is maintained 25 DEG C~40 DEG C, contain
Salt amount is below 3%, pH scopes between 6~8, the acetonitrile concentration≤50mg/L in sewage.
5. application according to claim 4, wherein, the sewage includes petrifaction sewage and/or other industry containing acetonitrile
The water body polluted by acetonitrile for producing.
6. application according to claim 4, wherein, the sewage is from the river, lake that are polluted by acetonitrile and/or sea
Ocean.
7. a kind of method that treatment contains the petrifaction sewage of acetonitrile, the method is using airlift bioreactor to containing acetonitrile
Petrifaction sewage is processed, wherein, activated sludge and can degrade described in claim 1 are inoculated with airlift bioreactor
The Shewanella putrefaciens of acetonitrile.
8. method according to claim 7, wherein, the Shewanella putrefaciens of the acetonitrile that can degrade described in claim 1 are
It is previously prepared into seed liquor, then with activated sludge with 1:3~3:1 volume ratio combined inoculation, inoculum concentration is biological anti-gas-lifting type
Answer the 1/6~1/3 of device basin's effective volume.
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| CN112342150B (en) * | 2019-08-06 | 2022-08-30 | 中国石油化工股份有限公司 | Petroleum degrading bacterium and application thereof |
| CN110656064A (en) * | 2019-10-24 | 2020-01-07 | 苏州市双利环保工程有限公司 | Shewanella putrefaciens and culture method and application thereof |
| CN111041072B (en) * | 2019-12-19 | 2021-02-12 | 北京师范大学 | Method for separating in-situ degrading bacteria of acetonitrile in wastewater by using magnetic nanoparticle technology |
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