CN101638628A - Production method for microbe flocculant - Google Patents
Production method for microbe flocculant Download PDFInfo
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- CN101638628A CN101638628A CN200910042265A CN200910042265A CN101638628A CN 101638628 A CN101638628 A CN 101638628A CN 200910042265 A CN200910042265 A CN 200910042265A CN 200910042265 A CN200910042265 A CN 200910042265A CN 101638628 A CN101638628 A CN 101638628A
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- microbial flocculant
- production method
- substratum
- culture medium
- flocculant
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Abstract
The invention discloses a production method for microbe flocculant, which comprises the following steps: eudomonas fluoresce is inoculated into culture medium by volume ratio according to inoculum size of 1.5-3.0:100 and cultured for 30-48h in the temperature of 25-30 DEG C to prepare microbe flocculant; according to the concentration of formulation constituents in culture medium, the raw materialformulation of the culture medium is as follows: 5-8g/L amylaceum, 0.50-1.75g/L of peptone, 0.5-2.0g/L of monopotassium phosphate, 0.5-5.0g/L of dipotassium phosphate, 100-300mg/L of magnesium sulfate, 200-700mg/L of sodium chloride, and 10-60mg/L of ferrous sulphate; the NaOH is used for regulating Ph to be 6.0-8.0. The microbe flocculant produced by the method can obtain high flocculating rateand few adding amount; and the microbe flocculant is used for water treatment, thus being efficient, simple, convenient, and cheap.
Description
Technical field
The present invention relates to a kind of microbial flocculant, particularly relate to the production method of microbial flocculant.
Background technology
Along with development of global economy, the progress of suitability for industrialized production, human standard of living improves greatly, but human environment of depending on for existence but suffers to destroy day by day the disorder of environment rule.The severe situation of water resources shortage and pollution aggravation has promoted the research and the application of water conditioner.On water conditioner flocculation technique development history, developed the flocculation agent (as Tai-Ace S 150, aluminum chloride, iron(ic) chloride etc.) of inorganic salts the sixties, its treatment effect is undesirable; And then the high molecular polymerization inorganic salt type flocculation agent (as polymerize aluminum chloride, bodied ferric sulfate etc.) of development has been received good effect in water treatment, but its aluminum ion of polymerize aluminum chloride in use can produce certain secondary pollution to environment, and can cause toxic effects such as senile dementia; Polyacrylamide is difficult to degraded in environment, easily cause secondary pollution, and has intensive neurotoxicity and " three cause " effect.
Microbial flocculant is as third generation organic polymer coargulator, be a kind of efficient, nontoxic, non-secondary pollution, can degrade voluntarily, flocculation agent of new generation that use range is wide.It is by directly utilizing microorganism cells, or cell extract, or meta-bolites extracts, the refining and material that flocculation activity is arranged that obtains.Microbial flocculant can overcome inorganic and the inherent cost of organic polymer coargulator height own, flocculating effect to be had and has secondary pollution and harmful defective.
Chinese invention patent application 200810073926.8 discloses a kind of immobilized preparation of flocculating microbial cell.It is bacterial classification that this method selects for use a plant height to imitate flocculating microbial, and at 30 ℃, 150r/min cultivated 24 hours, and the culture of strains base consists of glucose 20g, KH
2PO
42g, K
2HPO
45g, (NH
4)
2SO
40.2g, NaCl 0.1g, urea 0.5g, yeast extract paste 0.5g, MgSO
47H
2O 0.2g, distilled water 1000ml, pH 7.2-7.6; With liquid medium centrifugal 30min under 3000r/m, use the stroke-physiological saline solution dissolution precipitation, constant volume is to former scale, and is centrifugal under the same conditions, gets wet thallus; The sodium alginate of getting the 100-150ml mass percent concentration and be 2%-6% mixes with the 0.4-0.8g wet thallus and stirs; The preparation mass percent concentration is the calcium chloride solution 100ml-200ml of 7%-13%, place 0 ℃ mixture of ice and water, with syringe sodium alginate-bacterial classification mixed solution drop is splashed in the calcium chloride solution, form spherical particle, keep 1h-2h, make the abundant immobilization of bacterial classification at 10 ℃-25 ℃, remove supernatant liquor, spherical gel bacterial classification after the water flushing immobilization places calcium chloride solution balance 24h, i.e. the flocculating microbial cell particle of being fixed then again.Its flocculation activity storage rate reaches 88%-94%.But this method complex process is not easy to operational administrative, and the incubation time of this bacterium is longer, and this microbial flocculant production cycle is long.
Summary of the invention
The objective of the invention is to overcome above flocculation agent preparation method's deficiency, provide a kind of with short production cycle, flocculating effect is high, what can adapt to large-scale commercial production prepares the method for microbial flocculant by Pseudomonas fluorescens.
Have only on the one hand to have obtained to flocculate efficiently bacterial classification, could guarantee flocculation agent efficiently.So the present invention optimizes culture of strains base kind, PH, culture temperature, time, mixing speed, interpolation ionic species, concentration.
The microbial flocculant price is higher on the other hand, and in order to obtain high flocculating rate and few dosage, the present invention adds the inorganic cation ion as setting accelerator.
A kind of production method of microbial flocculant, in volume ratio, by 1.5-3.0: 100 inoculum size inserts Pseudomonas fluorescens in substratum, be 150-250rpm in shaking speed, and temperature is 25-30 ℃, cultivates 30-48h, makes microbial flocculant; Press the densitometer of system component in substratum, described culture medium raw material prescription is glucose 5-8g/L, peptone 0.50-1.75g/L, potassium primary phosphate 0.5-2.0g/L, dipotassium hydrogen phosphate 0.5-5.0g/L, sal epsom 100-300mg/L, sodium-chlor 200-700mg/L, ferrous sulfate 10-60mg/L regulates pH 6.0-8.0 with NaOH.
For further realizing the object of the invention, the concentration of described glucose in substratum is preferably 6-7g/L.
The concentration of described peptone in substratum is preferably 0.8-1.5g/L.
Described shaking speed is preferably 200-250rpm.
Described culture temperature is preferably 27-29 ℃.
Described incubation time is preferably 35-40h.
Described microbial flocculant also preferably includes calcium ion or iron ion; This microbial flocculant of every 1L contains the 300-500mg calcium ion; This microbial flocculant of every 1L contains the 500-800mg iron ion.
As follows with respect to prior art beneficial effect of the present invention:
(1) efficient, use this microbiobacterial agent, flocculating rate reaches more than 91%, but also has decolouring and deodorizing effect.
(2) nontoxic, through identifying that its flocculation composition is a polysaccharide, can be used for the fermentation aftertreatment of industries such as food, medicine, without any side effects to human body and animal.
(3) non-secondary pollution, the flocculation composition can be degraded voluntarily.
(4) easy and simple to handle, utilize this microbial flocculant to carry out water treatment, be easy to realize solid-liquid separation, settling efficiency height, to form throw out few, so have good turbidity removal and decolorization.Because its growth metabolism is fast, so can be used for large-scale industrialized production.
Embodiment
The present invention is further illustrated below in conjunction with embodiment, need to prove embodiment and the qualification of protection domain of the present invention not all in pairs.
Embodiment 1
A kind of production method of microbial flocculant, the culture medium raw material prescription calculates with composition concentration in raw material, is glucose 6g/L, peptone 1.20g/L, potassium primary phosphate 1.8g/L, dipotassium hydrogen phosphate 4.8g/L, sal epsom 180mg/L, sodium-chlor 19mg/L, ferrous sulfate 250mg/L regulates pH7.0 with NaOH, then in volume ratio, in substratum, insert Pseudomonas fluorescens (the photosynthetic bacteria liquid that the white biotechnology in Zhongshan city hundred company limited produces) by 1.5: 100 inoculum sizes, in shaking speed is 200rpm, and temperature is 25 ℃, cultivates 38h, stop to stir, treat its precipitation, the supernatant liquor that inclines, precipitation is desired microorganisms flocculation agent crude product; Its main component is a polysaccharide after measured, is secretion in this bacteria growing process.Get Zhujiang River limit sewage 1L, add this flocculation agent crude product 0.25mg.Measure flocculating rate.
Flocculation activity represents that with flocculating rate (FR) its measuring method is as follows: the Kaolin clay suspension and the 500ml 1%CaCl that prepare 1L 4g/L earlier
2Solution.Get the 100ml Kaolin clay suspension in the high pin beaker of 100ml, and then add 5ml 1%CaCl
2Solution and 1ml fermented liquid.Stir 1min earlier fast and stir 2min more at a slow speed, leave standstill 5min then.Get the supernatant liquor in the 60ml scale, with its absorbancy of spectrophotometric determination at the 550nm place.Replace fermented liquid to do controlled trial with deionized water simultaneously, calculate flocculating rate:
Flocculating rate=(A-B)/A * 100%
In the formula, A is the absorbance of contrast supernatant liquor at the 550nm place
B is the supernatant liquor of the sample that the adds fermented liquid absorbance at the 550nm place.
Add sewage behind this flocculation agent crude product than the obvious clarification that does not add, measure through aforesaid method, its flocculating rate FR reaches 90.94%.
Embodiment 2
A kind of production method of microbial flocculant, calculate with composition concentration in raw material, the culture medium raw material prescription is glucose 6.5g/L, peptone 1.50g/L, potassium primary phosphate 2.0g/L, dipotassium hydrogen phosphate 4.5g/L, sal epsom 200mg/L, sodium-chlor 20mg/L, ferrous sulfate 230mg/L regulates pH7.0 with NaOH, then in volume ratio, inserting Pseudomonas fluorescens (the photosynthetic bacteria liquid that the white biotechnology in Zhongshan city hundred company limited produces) by 1.5: 100 inoculum sizes in substratum, is 200rpm in shaking speed, and temperature is 25 ℃, cultivate 38h, stop to stir, wait for its precipitation, precipitation is desired microorganisms flocculation agent crude product; Its main component is a polysaccharide after measured, is secretion in this bacteria growing process.Get the sewage 1L that discharge in the dining room, add this flocculation agent crude product 0.20mg, measure flocculating rate.
The sewage that adds behind this flocculation agent crude product is more a lot of than the obvious clarification that does not add, and smell is also so unpleasant.Its flocculating rate FR reaches 91.98% after measured.
Embodiment 3
A kind of production method of microbial flocculant, calculate with composition concentration in raw material, the culture medium raw material prescription is glucose 7.0g/L, peptone 1.0g/L, potassium primary phosphate 1.8g/L, dipotassium hydrogen phosphate 5.0g/L, sal epsom 190mg/L, sodium-chlor 18mg/L, ferrous sulfate 250mg/L regulates pH6.5 with NaOH, then in volume ratio, inserting Pseudomonas fluorescens by 1.5: 100 inoculum sizes in substratum, is 200rpm in shaking speed, and temperature is 25 ℃, cultivate 38h, stop to stir, wait for its precipitation, precipitation is desired microorganisms flocculation agent crude product; Its main component is a polysaccharide after measured, is secretion in this bacteria growing process.Get the sewage 11 that discharge in the paper mill, add this flocculation agent crude product 0.30mg, measure flocculating rate.
The sewage that adds behind this flocculation agent crude product is more a lot of than the obvious clarification that does not add, and dark reddish purple look color also fades away a lot.Its flocculating rate FR reaches 91.98% after measured.
Embodiment 4
With the microbial flocculant of 0.20mg example 1 and the CaCl of 3ml 100mg/L
2With 6ml 100mg/L FeCl
3Solution mixes, and gets Zhujiang River limit sewage 1L, adds this mixed solution.And establish and do not add CaCl
2And FeCl
3The experiment contrast of solution is measured flocculating rate.
It is all a lot of than the obvious clarification that does not add that the flocculation agent that whether mixes adds sewage.But measure flocculating rate and find that the former flocculating rate FR reaches 92%, the latter has only 90.94%.
Embodiment 5
With the microbial flocculant of 0.25mg example 1 and the CaCl of 4ml 100mg/L
2With 5ml 100mg/L FeCl
3Solution mixes, and gets dining room sewage 1L, adds this mixed solution.And establish and do not add CaCl
2And FeCl
3The experiment contrast of solution is measured flocculating rate.
It is all a lot of than the obvious clarification that does not add that the flocculation agent that no matter whether mixes adds sewage, and smell is so not unpleasant yet.But measure flocculating rate and find that the former reaches 92.58% by flocculating effect, the latter is 90.94%.
Embodiment 6
With the microbial flocculant of 0.30mg example 1 and the CaCl of 3.5ml 100mg/L
2Solution and 7ml 100mg/L FeCl
3Mix, get paper mill sewer 1L, add this mixed solution.And establish and do not add CaCl
2And FeCl
3The experiment contrast of solution is measured flocculating rate.
It is all a lot of than the obvious clarification that does not add that the flocculation agent that no matter whether mixes adds sewage, and dark reddish purple look color also fades away a lot.But measure flocculating rate and find that the former flocculating rate FR reaches 92%, the latter is 90.94%.
Claims (7)
1, a kind of production method of microbial flocculant is characterized in that: in volume ratio, by 1.5-3.0: 100 inoculum size inserts Pseudomonas fluorescens in substratum, in shaking speed is 150-250rpm, temperature is 25-30 ℃, cultivates 30-48h, makes microbial flocculant; Press the densitometer of system component in substratum, described culture medium raw material prescription is glucose 5-8g/L, peptone 0.50-1.75g/L, potassium primary phosphate 0.5-2.0g/L, dipotassium hydrogen phosphate 0.5-5.0g/L, sal epsom 100-300mg/L, sodium-chlor 200-700mg/L, ferrous sulfate 10-60mg/L regulates pH 6.0-8.0 with NaOH.
2, the production method of microbial flocculant according to claim 1 is characterized in that the concentration of described glucose in substratum is 6-7g/L.
3, the production method of microbial flocculant according to claim 1 is characterized in that the concentration of described peptone in substratum is 0.8-1.5g/L.
4, require the production method of 1 described microbial flocculant according to profit, it is characterized in that described shaking speed is 200-250rpm.
5, the production method of microbial flocculant according to claim 1 is characterized in that described culture temperature is 27-29 ℃.
6, the production method of microbial flocculant according to claim 1 is characterized in that described incubation time is 35-40h.
7, the production method of microbial flocculant according to claim 1 is characterized in that described microbial flocculant also comprises calcium ion or iron ion; This microbial flocculant of every 1L contains the 300-500mg calcium ion; This microbial flocculant of every 1L contains the 500-800mg iron ion.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN102061313A (en) * | 2010-11-11 | 2011-05-18 | 王紫 | Production method of bioflocculant fermentation liquor and flocculant special for drinking water during flood fighting and disaster relieving and application thereof |
| CN102557211A (en) * | 2011-12-20 | 2012-07-11 | 长安大学 | Multi-functional composite bioflocculant for wastewater treatment |
| CN102706841A (en) * | 2012-05-07 | 2012-10-03 | 清华大学 | Indoor cultivation and observation method for bio-flocculation sediment |
| CN103911395A (en) * | 2013-12-29 | 2014-07-09 | 新疆德蓝股份有限公司 | Ferric-calcium-based compound bioflocculant and preparation method thereof |
| CN103937696A (en) * | 2013-12-27 | 2014-07-23 | 新疆德蓝股份有限公司 | Biological flocculant producing strain and its fermentation preparation method |
| CN104531583A (en) * | 2014-12-30 | 2015-04-22 | 盐城工学院 | Pseudomonas and microbial flocculant formed by fermenting pseudomonas |
| CN104556405A (en) * | 2014-12-30 | 2015-04-29 | 盐城工学院 | Biologically compounded flocculant containing ferric ions and application of biologically compounded flocculant |
| CN104593351A (en) * | 2014-12-30 | 2015-05-06 | 安徽丰原发酵技术工程研究有限公司 | Method for preparing cephalosporin C acylase immobilized enzyme |
| CN106119144A (en) * | 2016-04-11 | 2016-11-16 | 江苏师范大学 | A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant |
| CN107759042A (en) * | 2016-08-19 | 2018-03-06 | 辽宁惠源生物环保科技有限公司 | A kind of complex micro organism fungicide for mud decrement and preparation method thereof |
| CN113025536A (en) * | 2021-04-28 | 2021-06-25 | 桂林理工大学 | Pseudomonas PR1 and preparation method and application thereof |
| CN115521959A (en) * | 2022-10-12 | 2022-12-27 | 江苏力凡胶囊有限公司 | Extraction method of pullulan |
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2009
- 2009-08-28 CN CN200910042265A patent/CN101638628A/en active Pending
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN101870958B (en) * | 2010-05-10 | 2012-05-23 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN102061313A (en) * | 2010-11-11 | 2011-05-18 | 王紫 | Production method of bioflocculant fermentation liquor and flocculant special for drinking water during flood fighting and disaster relieving and application thereof |
| CN102557211A (en) * | 2011-12-20 | 2012-07-11 | 长安大学 | Multi-functional composite bioflocculant for wastewater treatment |
| CN102557211B (en) * | 2011-12-20 | 2013-07-17 | 长安大学 | Multi-functional composite bioflocculant for wastewater treatment |
| CN102706841A (en) * | 2012-05-07 | 2012-10-03 | 清华大学 | Indoor cultivation and observation method for bio-flocculation sediment |
| CN103937696A (en) * | 2013-12-27 | 2014-07-23 | 新疆德蓝股份有限公司 | Biological flocculant producing strain and its fermentation preparation method |
| CN103911395A (en) * | 2013-12-29 | 2014-07-09 | 新疆德蓝股份有限公司 | Ferric-calcium-based compound bioflocculant and preparation method thereof |
| CN104531583A (en) * | 2014-12-30 | 2015-04-22 | 盐城工学院 | Pseudomonas and microbial flocculant formed by fermenting pseudomonas |
| CN104556405A (en) * | 2014-12-30 | 2015-04-29 | 盐城工学院 | Biologically compounded flocculant containing ferric ions and application of biologically compounded flocculant |
| CN104593351A (en) * | 2014-12-30 | 2015-05-06 | 安徽丰原发酵技术工程研究有限公司 | Method for preparing cephalosporin C acylase immobilized enzyme |
| CN104593351B (en) * | 2014-12-30 | 2018-03-13 | 安徽丰原发酵技术工程研究有限公司 | A kind of method for preparing cephalosporin C acrylase immobilised enzymes |
| CN106119144A (en) * | 2016-04-11 | 2016-11-16 | 江苏师范大学 | A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant |
| CN106119144B (en) * | 2016-04-11 | 2019-07-26 | 江苏师范大学 | A kind of conversion peanut shell hydrolysate prepares the method and bacterial strain of microbial flocculant |
| CN107759042A (en) * | 2016-08-19 | 2018-03-06 | 辽宁惠源生物环保科技有限公司 | A kind of complex micro organism fungicide for mud decrement and preparation method thereof |
| CN113025536A (en) * | 2021-04-28 | 2021-06-25 | 桂林理工大学 | Pseudomonas PR1 and preparation method and application thereof |
| CN113025536B (en) * | 2021-04-28 | 2022-11-25 | 桂林理工大学 | Pseudomonas PR1 and preparation method and application thereof |
| CN115521959A (en) * | 2022-10-12 | 2022-12-27 | 江苏力凡胶囊有限公司 | Extraction method of pullulan |
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