CN106119144A - A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant - Google Patents
A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant Download PDFInfo
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- CN106119144A CN106119144A CN201610220636.6A CN201610220636A CN106119144A CN 106119144 A CN106119144 A CN 106119144A CN 201610220636 A CN201610220636 A CN 201610220636A CN 106119144 A CN106119144 A CN 106119144A
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- Prior art keywords
- hydrolysate
- arachidis hypogaeae
- pericarppium arachidis
- microbial flocculant
- flocculant
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 45
- 239000000413 hydrolysate Substances 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 32
- 230000004151 fermentation Effects 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 239000002609 medium Substances 0.000 claims abstract description 16
- 239000006228 supernatant Substances 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000001816 cooling Methods 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 9
- 235000019628 coolness Nutrition 0.000 claims abstract description 7
- 229960000935 dehydrated alcohol Drugs 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- 239000002244 precipitate Substances 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 11
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 8
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 8
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 7
- 238000001556 precipitation Methods 0.000 claims description 7
- 229920002472 Starch Polymers 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 5
- 244000105624 Arachis hypogaea Species 0.000 claims description 5
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 5
- 235000018262 Arachis monticola Nutrition 0.000 claims description 5
- 235000020232 peanut Nutrition 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 239000005018 casein Substances 0.000 claims description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 4
- 235000021240 caseins Nutrition 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 4
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims 1
- 108010080698 Peptones Proteins 0.000 claims 1
- 102000004142 Trypsin Human genes 0.000 claims 1
- 108090000631 Trypsin Proteins 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 235000019319 peptone Nutrition 0.000 claims 1
- 239000012588 trypsin Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000003311 flocculating effect Effects 0.000 description 4
- 238000005189 flocculation Methods 0.000 description 4
- 230000016615 flocculation Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000012137 tryptone Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108010076119 Caseins Proteins 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 241000589516 Pseudomonas Species 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000008394 flocculating agent Substances 0.000 description 3
- 239000010903 husk Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000002154 agricultural waste Substances 0.000 description 2
- 159000000013 aluminium salts Chemical class 0.000 description 2
- 229910000329 aluminium sulfate Inorganic materials 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
- 150000002505 iron Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 229920000620 organic polymer Polymers 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 235000010413 sodium alginate Nutrition 0.000 description 2
- 239000000661 sodium alginate Substances 0.000 description 2
- 229940005550 sodium alginate Drugs 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 239000004368 Modified starch Substances 0.000 description 1
- 206010029350 Neurotoxicity Diseases 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 206010044221 Toxic encephalopathy Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 108010079058 casein hydrolysate Proteins 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000002337 glycosamines Chemical class 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 description 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 230000007135 neurotoxicity Effects 0.000 description 1
- 231100000228 neurotoxicity Toxicity 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000013316 zoning Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
Abstract
The present invention relates to a kind of Pericarppium arachidis hypogaeae hydrolysate that converts and prepare method and the bacterial strain of microbial flocculant, belong to microbial preparation field.Preparation technology is as follows: by strainPseudomonas sp.BWL918 is inoculated in seed liquor culture medium, and preparation seed liquor cultivated by 28 DEG C of shaking tables；Seed liquor being transferred in the fermentation medium with Pericarppium arachidis hypogaeae hydrolysate as carbon source in 1% ratio, 28 DEG C, 48 h cultivated by 180 rpm shaking tables；By fermentation liquid at 4 DEG C, after 10000 rpm are centrifuged 10 min, collect supernatant and be liquid microbe flocculant；Adding the dehydrated alcohol of 2 times of volume pre-coolings in liquid fermentation liquid, centrifugal collecting precipitate lyophilization obtain sterling microbial flocculant.The present invention can utilize Pericarppium arachidis hypogaeae hydrolysate to produce microbial flocculant as the carbon source of fermentation medium, can reduce the production cost of microbial flocculant, adapt to large-scale production and industrial applications.
Description
Technical field
The present invention relates to a kind of Pericarppium arachidis hypogaeae hydrolysate that converts and prepare method and the bacterial strain of microbial flocculant, be specifically related to profit
WithPseudomonas Sp.BWL918 converts the method for Pericarppium arachidis hypogaeae hydrolysate fermenting and producing microbial flocculant.
Background technology
China is large agricultural country, can produce the agricultural wastes of about 700,000,000 tons, such as corn stalk in annual agricultural production process
Stalk, Pericarppium arachidis hypogaeae, soil dynamic test and rice husk etc., people are usually taken burn processing, but this not only wastes resource, and pollute ring
Border.Containing abundant cellulose substances in these residues, saccharide can be changed into, thus produce other high value added products,
The most effectively these cellulose family agricultural wastes are converted into value product and there is important economic implications.
Flocculant is that a class can assemble the material of particle in sedimentation solution, is broadly divided into inorganic flocculating agent (such as sulfur
Acid aluminum, bodied ferric sulfate etc.), organic polymer coargulator (such as polymine, polyacrylamide etc.) and natural polymer wad a quilt with cotton
Solidifying agent (such as modified starch, chitosan, sodium alginate, chitin and microbial flocculant etc.).Inorganic flocculating agent is in China market
On account for important share, have the demand of hundreds of thousands ton every year, mainly with iron salt, aluminium salt is representative.Iron salt has corrosivity, easily
Forming residual, the water after process, with color, causes secondary pollution；Find on hygiology that aluminium salt accumulation in human body is with current
Senile dementia has direct relation.Organic polymer coargulator extensively should due to its good flocculating effect and low cost
In actual production, with polyacrylamide as representative, but its difficult degradation, monomeric acrylamide is a kind of strong carcinogen, and
There is strong neurotoxicity, residue in the acrylamide monomer in environment and easily cause secondary pollution, the environmentally safe and mankind
Health causes and has a strong impact on.Although the natural macromolecule flocculating agents such as chitosan, sodium alginate, chitin are nontoxic, energy safe disposal,
But its flocculation activity is more weak, relatively costly, limits it and extensively apply；Microbial flocculant is novel third generation flocculant,
It is the metabolite produced by microorganism, such as glycoprotein, mucopolysaccharide, protein, cellulose etc., has nontoxic, safety
Height, the feature such as biological degradable and non-secondary pollution, therefore microbial flocculant can be widely used in sewage disposal, especially
Being the advantage in the technique such as post processing of food and fermentation industry with uniqueness, its research is increasingly subject to pay attention to.
The bacterium for producing flocculant of microbe strain reported for work at present has tens kinds, such as Bacillus licheniformis X14
The microbial flocculant produced mainly is made up of neutral sugar and protein, and its Optimal compositions of fermentation medium is glucose 20 g/L, ferment
Female powder 0.5g/L, carbamide 0.5g/L；Bacillus firmusS-14 can produce a kind of polysaccharide biological flocculant, its
Optimal compositions of fermentation medium is glucose 10 g/L, yeast powder 0.5g/L；BacillusSp. the biological flocculant that F19 is produced
Mainly being made up of neutral sugar, alduronic acid, amino sugar and protein etc., its Optimal compositions of fermentation medium is sucrose 20 g/L, yeast powder
2.5 g/L.These strain used mediums are many using expensive saccharide as key component, cause microbial flocculant production cost
Height, limits the industrial applications of microbial flocculant.
In order to reduce the production cost of microbial flocculant, Guo etc. utilizes corn straw hydrolysate as bacterial strainRhodococcuserythropolisFermenting carbon source produce microbial flocculant (Bioresource
Technology. 2015;177:393-397)；Wang etc. utilize rice husk hydrolysate as bacterial strainOchrobactiumciceriThe fermenting carbon source of W2 produces microbial flocculant (Bioresource
Technology, 2013,145:259-263)；Chin-Hang Shu et al. also utilizes rice husk hydrolysate conductSchizophyllum communeCulture medium produce microbial flocculant (Journal of the Taiwan
Institute of Chemical Engineers, 2011, 42(3): 387-393).But have no at present and utilize Pericarppium arachidis hypogaeae water
Solve thing and produce the research of microbial flocculant as fermentation medium.The annual peanut yield of China about more than 1,500 ten thousand tons, occupies the world
First, the annual Pericarppium arachidis hypogaeae garbage producing about 5,000,000 tons, these Pericarppium arachidis hypogaeae garbages are commonly used as fuel, cause resource
Waste and environmental pollution.Containing materials such as abundant cellulose, starch in Pericarppium arachidis hypogaeae, have and develop other high value added products
Potentiality.Bacterial strain uses therefor of the present invention is that we separate the pseudomonas of acquisitionPseudomonas Sp.BWL918, this bacterial strain energy
Utilize Pericarppium arachidis hypogaeae hydrolysate fermenting and producing microbial flocculant, the production cost of microbial flocculant can be effectively reduced, become
Waste be changed into values, it is achieved the recycling of Pericarppium arachidis hypogaeae.
Summary of the invention
The invention provides onePseudomonas Sp.BWL918 bacterial strain and convert Semen arachidis hypogaeae in neutral conditions
Shell hydrolysate produces the method for high-activity microorganism flocculant.This bacterial strain has fast growth, and fermentation period is short, and flocculant produces
Amount advantages of higher, it is possible to be effectively reduced the production cost of microbial flocculant.
The present invention includes herein below: a kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares the bacterial strain of microbial flocculantPseudomonas Sp.BWL918, is preserved in China General Microbiological culture presevation administrative center, and preservation address is Beijing
North Star West Road, Chaoyang District, city 1 institute 3, culture presevation number is CGMCC 11994, preservation date 2016.01.12.
One utilizes bacterial strainPseudomonas Sp.BWL918 converts Pericarppium arachidis hypogaeae hydrolysate and prepares microbial flocculant
Method, the processing step of the method is as follows:
Step 1, Pericarppium arachidis hypogaeae is pulverized, and sieve with 60 mesh mesh screens, by the concentration of 100g/L, peanut hull meal is added 1.7%(W/W)
H2SO4In solution, hydrolyze 120 min in 121 DEG C, after cooling, centrifugal 10 min, collect supernatant, with Ca (OH)2Regulation pH
To 7.0, recentrifuge 10 min, collects supernatant and obtains the Pericarppium arachidis hypogaeae hydrolysate carbon source for subsequent fermentation culture medium；
Step 2, being inoculated into by strain in seed liquor culture medium, 12 h cultivated by 28 DEG C of shaking tables, prepare seed liquor；
Step 3, seed liquor being transferred to using Pericarppium arachidis hypogaeae hydrolysate as the fermentation medium of carbon source in the ratio of 1%, shaking table is trained
Support 48 h；
Step 4, by fermentation medium at 4 DEG C, centrifugal 10 min, collect supernatant and be liquid microbe flocculant；
Step 5, the dehydrated alcohol of 2 times of volume pre-coolings of addition, centrifugal collecting precipitate, and freezing in liquid microbe flocculant
It is dried to obtain microbial flocculant product.
Further, the seed liquor culture medium described in step 2 is glucose 1 g/L, soluble starch 1 g/L, yeast
Powder 1 g/L, tryptone 1 g/L, acid hydrolyzed casein 1 g/L, Magnesium sulfate heptahydrate 0.1 g/L, dipotassium hydrogen phosphate 0.6 g/L,
The cultivation temperature of seed liquor is 28 DEG C.
Further, the shaking table condition of culture in step 3 is 28 DEG C, and 48 h cultivated by 180 rpm shaking tables.
Further, the fermentation medium described in step 3 is Pericarppium arachidis hypogaeae hydrolysate 300mL/L, yeast powder 3 g/L, seven
Water magnesium sulfate 0.1 g/L, dipotassium hydrogen phosphate 0.6 g/L, the initial pH of fermentation liquid are 7.0.
Further, the centrifugal rotational speed of step 1 and step 4 is 10000 rpm.
Further, in described step 5, in the liquid microbe flocculant that step 4 obtains, add 2 times of volume pre-coolings
Dehydrated alcohol, 10000 rpm be centrifuged 10 min collect precipitation, 70% washing with alcohol precipitation after, lyophilization obtain microorganism wadding
Solidifying agent sterling.
The present invention compared with prior art, has the advantage that present invention firstly provides and utilizes Pericarppium arachidis hypogaeae hydrolysate to produce
Microbial flocculant, it is possible to achieve the recycling to Pericarppium arachidis hypogaeae, reduces environmental pollution.Bacterial strain involved in the present inventionPseudomonas Sp.BWL918 fast growth, fermentation period is short, and microbial flocculation yield is high, well should have
Use prospect.
Detailed description of the invention
A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares the bacterial strain of microbial flocculantPseudomonas Sp.BWL918,
Being preserved in China General Microbiological culture presevation administrative center, culture presevation number is CGMCC 11994.
A kind of convert Pericarppium arachidis hypogaeae hydrolysate to prepare the processing step of microbial flocculant as follows:
Step 1, by strainPseudomonas Sp., during BWL918 is inoculated into seed liquor culture medium, 28 DEG C of shaking flasks are overnight trained
Support preparation seed liquor；
Step 2, seed liquor is transferred in the fermentation medium with Pericarppium arachidis hypogaeae hydrolysate as carbon source in 1% ratio, 28 DEG C, 180
48 h cultivated by rpm shaking table；
Step 3, by fermentation medium at 4 DEG C, 10000 rpm are centrifuged 10 min, remove precipitation, and supernatant is liquid bio wadding
Solidifying agent；
Step 4, in liquid fermentation liquid add 2 times of volume pre-coolings dehydrated alcohol, centrifugal collecting precipitate lyophilization obtain
To sterling biological flocculant.
In described step 1, strain used is pseudomonasPseudomonas Sp.BWL918, patent culture presevation
Number it is CGMCC 11994, Pericarppium arachidis hypogaeae hydrolysate can be utilized to produce microbial flocculant；
In described step 1, seed liquor medium component is as follows: glucose 1 g/L, soluble starch 1 g/L, yeast powder 1 g/
L, tryptone 1 g/L, acid hydrolyzed casein 1 g/L, Magnesium sulfate heptahydrate 0.1 g/L, dipotassium hydrogen phosphate 0.6 g/L；
In described step 2, the preparation process of Pericarppium arachidis hypogaeae hydrolysate is as follows: pulverized by Pericarppium arachidis hypogaeae pulverizer, with 60 mesh mesh screens
Sieve, by the concentration of 100g/L, peanut hull meal added 1.7%(W/W) H2SO4In solution, 121 DEG C of hydrolysis 120 min, cooling
After, centrifugal collection supernatant, with Ca (OH)2Regulation pH to 7, recentrifuge is collected supernatant and is obtained Pericarppium arachidis hypogaeae hydrolysate；
In described step 2, the composition of fermentation medium is as follows: Pericarppium arachidis hypogaeae hydrolysate 300 mL/L, yeast powder are nitrogen source 3 g/
L, Magnesium sulfate heptahydrate 0.1 g/L, dipotassium hydrogen phosphate 0.6 g/L.
Specific embodiment 1: microbial flocculant preparation method, specifically comprises the following steps that
1, strain separating and qualification: utilize gradient dilution method separation screening function stem, described screening and culturing based component is: Portugal
Grape sugar 0.5 g/L, soluble starch 0.5 g/L, yeast powder 0.5 g/L, tryptone 0.5 g/L, acid hydrolyzed casein 0.5
G/L, Magnesium sulfate heptahydrate 0.05g/L, dipotassium hydrogen phosphate 0.3g/L.After strain separating obtains, obtained by four zoning collimation method method of purification
To purebred bacterial strain, through 16S rRNA sequence analysis determine strain used by the present invention be pseudomonas (Pseudomonas
And it is named sp.),Pseudomonas Sp. BWL918, patent culture presevation number is CGMCC 11994。
2, prepared by seed liquor: by pseudomonasPseudomonas Sp.BWL918 is seeded in seed liquor culture medium,
Seed liquor nutrient media components is as follows: glucose 1 g/L, soluble starch 1 g/L, yeast powder 1 g/L, tryptone 1 g/L, acid
Caseinhydrolysate 1 g/L, Magnesium sulfate heptahydrate 0.1 g/L, dipotassium hydrogen phosphate 0.6 g/L.Then 28 DEG C, 180 rpm incubator overnight
Cultivate.
3, microbial flocculant is prepared in fermentation: seed liquor step 2 obtained is seeded in fermentation medium in 1% ratio,
Fermentation medium component is as follows: peanut hull meal hydrolysate 300mL/L;Yeast powder 3 g/L, Magnesium sulfate heptahydrate 0.1 g/L, phosphoric acid
Hydrogen dipotassium 0.6 g/L；Then 28 DEG C, 180rpm shaker fermentation cultivates 48h.
4, microbial flocculant extracts: fermentation liquid step 3 obtained is centrifugal 10 min under the conditions of 10000 rpm, receive
Collection supernatant；Adding the dehydrated alcohol of 2 times of volume pre-coolings in supernatant, 10000 rpm are centrifuged 10 min and collect precipitation, use
After 70% washing with alcohol precipitation, lyophilization obtains microbial flocculant sterling.
5, flocculation activity measures: add 100 μ L fermentation liquids in 60 mL5 g/L Kaolin suspensions；Quickly stirring 2
Min, is then slowly stirred 1 min, standing sedimentation 1 min, takes supernatant 722 type spectrophotometric determination OD550Value, to add
Enter 100 μ L distilled water as comparison, represent flocculation activity by calculating flocculating rate.Flocculating rate=(A-B)/A × 100%, A represents
The OD of supernatant when adding distilled water550Value, B represents with the OD of supernatant during fermentation liquid550Value.The microbial flocculant pair obtained
Aqueous suspension ofkaolin has obvious flocculating effect.
Claims (7)
1. one kind converts Pericarppium arachidis hypogaeae hydrolysate and prepares the bacterial strain of microbial flocculantPseudomonas Sp.BWL918, preservation
In China General Microbiological culture presevation administrative center, culture presevation number is CGMCC 11994.
2. the method that conversion Pericarppium arachidis hypogaeae hydrolysate as claimed in claim 1 prepares microbial flocculant, the work of the method
Skill step is as follows:
Step 1, Pericarppium arachidis hypogaeae is pulverized, and sieve with 60 mesh mesh screens, by the concentration of 100g/L, peanut hull meal is added 1.7%(W/W)
H2SO4In solution, hydrolyze 120 min in 121 DEG C, after cooling, centrifugal 10 min, collect supernatant, with Ca (OH)2Regulation pH
To 7.0, recentrifuge 10 min, collects supernatant and obtains the Pericarppium arachidis hypogaeae hydrolysate carbon source for subsequent fermentation culture medium；
Step 2, being inoculated into by strain in seed liquor culture medium, 28 DEG C of shaking tables are cultivated 12 h and are prepared seed liquor；
Step 3, seed liquor being transferred to using Pericarppium arachidis hypogaeae hydrolysate as the fermentation medium of carbon source in the ratio of 1%, shaking table is trained
Support 48 h；
Step 4, by fermentation medium at 4 DEG C, centrifugal 10 min, collect supernatant and be liquid microbe flocculant；
Step 5, the dehydrated alcohol of 2 times of volume pre-coolings of addition, centrifugal collecting precipitate, and freezing in liquid microbe flocculant
It is dried to obtain microbial flocculant product.
The most according to claim 1 a kind of converting the method that Pericarppium arachidis hypogaeae hydrolysate prepares microbial flocculant, its feature exists
In, the seed liquor culture medium described in step 2 is glucose 1 g/L, soluble starch 1 g/L, yeast powder 1 g/L, Trypsin
Peptone 1 g/L, acid hydrolyzed casein 1 g/L, Magnesium sulfate heptahydrate 0.1 g/L, dipotassium hydrogen phosphate 0.6 g/L, the cultivation temperature of seed liquor
Degree is 28 DEG C.
The most according to claim 1 a kind of converting the method that Pericarppium arachidis hypogaeae hydrolysate prepares microbial flocculant, its feature exists
In, the shaking table condition of culture in step 3 is 28 DEG C, and 48 h cultivated by 180 rpm shaking tables.
The most according to claim 1 a kind of converting the method that Pericarppium arachidis hypogaeae hydrolysate prepares microbial flocculant, its feature exists
In, the fermentation medium described in step 3 is Pericarppium arachidis hypogaeae hydrolysate 300mL/L, yeast powder 3 g/L, Magnesium sulfate heptahydrate 0.1 g/
L, dipotassium hydrogen phosphate 0.6 g/L, the initial pH of fermentation liquid are 7.0.
The most according to claim 1 a kind of converting the method that Pericarppium arachidis hypogaeae hydrolysate prepares microbial flocculant, its feature exists
In, the centrifugal rotational speed of step 1 and step 4 is 10000 rpm.
The most according to claim 1 a kind of converting the method that Pericarppium arachidis hypogaeae hydrolysate prepares microbial flocculant, its feature exists
In, in described step 5, in the liquid microbe flocculant that step 4 obtains, add the dehydrated alcohol of 2 times of volume pre-coolings,
10000 rpm are centrifuged 10 min and collect precipitation, and after 70% washing with alcohol precipitation, lyophilization obtains microbial flocculant sterling.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107815548A (en) * | 2017-11-09 | 2018-03-20 | 合山锌业科技有限公司 | The method that zinc cadmium waste residue produces feed grade monohydrate zinc sulphate and cadmium |
| CN113173646A (en) * | 2021-01-11 | 2021-07-27 | 武夷学院 | Microbial flocculant and preparation method and application thereof |
| CN115232767A (en) * | 2022-07-19 | 2022-10-25 | 山东大湖新材料有限公司 | Method for preparing bioflocculant by Parasphingorabhadu strain |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101638628A (en) * | 2009-08-28 | 2010-02-03 | 华南理工大学 | Production method for microbe flocculant |
| CN102363793A (en) * | 2011-10-25 | 2012-02-29 | 杭州江南科学研究院有限公司 | Preparation method of photosynthetic bacteria flocculant |
| CN102925489A (en) * | 2012-10-10 | 2013-02-13 | 临安天川环保科技有限公司 | Preparation method of microbial flocculant |
| CN102978274A (en) * | 2012-12-09 | 2013-03-20 | 哈尔滨工业大学宜兴环保研究院 | Method for applying anaerobic dry fermentation technology to treating rice straws to prepare biological flocculating agent |
| CN103525870A (en) * | 2013-10-23 | 2014-01-22 | 嘉兴学院 | Microbial flocculant as well as preparation method and application thereof |
| CN103937696A (en) * | 2013-12-27 | 2014-07-23 | 新疆德蓝股份有限公司 | Biological flocculant producing strain and its fermentation preparation method |
| CN104531583A (en) * | 2014-12-30 | 2015-04-22 | 盐城工学院 | Pseudomonas and microbial flocculant formed by fermenting pseudomonas |
| CN104830910A (en) * | 2015-05-25 | 2015-08-12 | 江苏师范大学 | Method for manufacturing microbial flocculants from cellulosimicrobium cellulans |
-
2016
- 2016-04-11 CN CN201610220636.6A patent/CN106119144B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101638628A (en) * | 2009-08-28 | 2010-02-03 | 华南理工大学 | Production method for microbe flocculant |
| CN102363793A (en) * | 2011-10-25 | 2012-02-29 | 杭州江南科学研究院有限公司 | Preparation method of photosynthetic bacteria flocculant |
| CN102925489A (en) * | 2012-10-10 | 2013-02-13 | 临安天川环保科技有限公司 | Preparation method of microbial flocculant |
| CN102978274A (en) * | 2012-12-09 | 2013-03-20 | 哈尔滨工业大学宜兴环保研究院 | Method for applying anaerobic dry fermentation technology to treating rice straws to prepare biological flocculating agent |
| CN103525870A (en) * | 2013-10-23 | 2014-01-22 | 嘉兴学院 | Microbial flocculant as well as preparation method and application thereof |
| CN103937696A (en) * | 2013-12-27 | 2014-07-23 | 新疆德蓝股份有限公司 | Biological flocculant producing strain and its fermentation preparation method |
| CN104531583A (en) * | 2014-12-30 | 2015-04-22 | 盐城工学院 | Pseudomonas and microbial flocculant formed by fermenting pseudomonas |
| CN104830910A (en) * | 2015-05-25 | 2015-08-12 | 江苏师范大学 | Method for manufacturing microbial flocculants from cellulosimicrobium cellulans |
Non-Patent Citations (1)
| Title |
|---|
| 杨莉等: "花生壳常压酸水解制备乙酰丙酸", 《花生学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107815548A (en) * | 2017-11-09 | 2018-03-20 | 合山锌业科技有限公司 | The method that zinc cadmium waste residue produces feed grade monohydrate zinc sulphate and cadmium |
| CN113173646A (en) * | 2021-01-11 | 2021-07-27 | 武夷学院 | Microbial flocculant and preparation method and application thereof |
| CN113173646B (en) * | 2021-01-11 | 2022-07-05 | 武夷学院 | Microbial flocculant and preparation method and application thereof |
| CN115232767A (en) * | 2022-07-19 | 2022-10-25 | 山东大湖新材料有限公司 | Method for preparing bioflocculant by Parasphingorabhadu strain |
| CN115232767B (en) * | 2022-07-19 | 2023-04-07 | 山东大湖新材料有限公司 | Method for preparing bioflocculant by Parasphingorabhubus sp |
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