CN102363793A - Preparation method of photosynthetic bacteria flocculant - Google Patents
Preparation method of photosynthetic bacteria flocculant Download PDFInfo
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- CN102363793A CN102363793A CN2011103268615A CN201110326861A CN102363793A CN 102363793 A CN102363793 A CN 102363793A CN 2011103268615 A CN2011103268615 A CN 2011103268615A CN 201110326861 A CN201110326861 A CN 201110326861A CN 102363793 A CN102363793 A CN 102363793A
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- flocculant
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- culture medium
- flocculation agent
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Abstract
The invention relates to a preparation method of a microbial flocculant, and discloses a preparation method of a photosynthetic bacteria flocculant, and the application of the same. The preparation method comprises the following steps of: culturing in a culture medium, selecting the Rhodopseudanonaspalustris as the original strain, wherein the culture collection number is CGMCC(China General Microbiological Culture Collection Center)1.2181, firstly, preparing the culture medium, then, extracting the flocculant, and obtaining white solid powder finished products of the flocculant. The Rhodopseudanonaspalustris cannot vary under the influence of the environment and has steady performance, the flocculant prepared by the Rhodopseudanonaspalustris has better storage performance and can be stored for one year at normal temperature (below 380 DEG C), and the flocculation efficiency of the flocculant can be more than 95%; and the Rhodopseudanonaspalustris flocculant prepared with the bacterium culture medium can be prepared through simple method with low cost within short fermentation cycle, so that the flocculant is suitable for large scale production.
Description
Technical field
The present invention relates to a kind of preparation method of microbial flocculant, related in particular to a kind of preparation method of photosynthetic bacterium flocculation agent.
Background technology
For a long time, found that for a long time some mikrobes have cell flocculation phenomenon, pay attention to but it is produced always, in recent years, the cell flocculation technique as a kind of easy and economic stripping technique continuously ferment and product separation in be widely used.
The microbe species that can produce microbial flocculant is a lot; They are present in the active sludge of soil and wastewater treatment in a large number; In addition, many many bacteriums that are used for industrial bacterial classification and some DSMZs also can produce microbial flocculant efficiently.
The effective constituent of microbial flocculant is the meta-bolites of flocculated bacteria, and what of meta-bolites are the power of flocculation ability depend on.In general, the effective constituent of flocculation agent should be and gathers polysaccharide and gp etc.
People such as Kurane utilize rhodococcus in the photosynthetic bacterium (
Rhodococcuserythropolis) succeed in developing biological flocculant NOC-1, slime water, river, water of coal ash, activated carbon powder water, bulking sludge, paper pulp wastewater etc. all there are fabulous flocculation and decolorizing effect, be one of best microbial flocculant of finding at present.
The present invention be with the Rhodopseudomonas palustris in the photosynthetic bacterium (
Rhodop seudanonas palustris) the flocculation agent WkF that produces by starting strain is for the preparation target, since the 1980s, Rhodopseudomonas palustris Ceng Zuowei photosynthetic bacterium a kind of once was in the news and is used for high-concentration waste water and handles.The characteristics of this bacterium, under illumination and anaerobic condition, liquid presents redness, and under unglazed photograph and aerobic condition, the liquid non-pigment, and the reproduction speed of thalline is multiplied.The present invention prepares microbial flocculant efficiently in the reproduction speed of unglazed photograph and aerobic condition hypothallus characteristics fast and that meta-bolites is many.
Domestic similar techniques:
(1) people's such as Nie Maiqian of Xi'an University of Architecture and Technology patent of invention " a kind of working method of microbial flocculant and method of use " (application for a patent for invention numbers 200810017351.8; Publication number CN101225405A), this patent of invention be with klebsiella a mutation (
Klebsiella pneumoniae) prepare microbial flocculant as starting strain.
(2) Hunan University is happy and carefree waits people's patent of invention " to utilize the production bacterium and the production technique thereof of Dregs Manufacture microbial flocculant " (application for a patent for invention number 200610031485.6, publication number CN1844360A), with Paenibacillus polymyxa (
PaenibacilluspolymyxaGA1 CCTCC M206017) inserts liquid nutrient medium, adopt two sections fermentation culture methods, the preparation microbial flocculant.
Summary of the invention
The purpose of this invention is to provide a kind of stable performance, institute's produce flocculant storage property is good, and flocculation efficiency is high, and the preparation method is easy, and fermentation period is short, and cost is low, is applicable to the preparation method of the photosynthetic bacterium flocculation agent of scale operation.
In order to solve the problems of the technologies described above, the present invention is able to solve through following technical proposals:
A kind of preparation method of photosynthetic bacterium flocculation agent is characterized in that, concrete grammar is following:
Select for use Rhodopseudomonas palustris (
Rhodop seudanonas palustris) be starting strain, culture presevation numbering: CGMCC1.2181, preservation place: Chinese common micro-organisms culture presevation administrative center; This bacterial classification is available from Institute of Microorganism, Academia Sinica (national culture presevation the council).
A, preparing culture medium:
1) culture presevation substratum: (NH
4)
2SO
41g, MgSO
4﹒ 7H
2O 0.2g, NaHCO
35g, K
2HPO
40.5g, NaCl 0.2g, peptone 1.5g, zero(ppm) water adds to 1L, agar 15.0g, pH7.0; 10
5The Pa 15min that sterilizes;
2) seed culture medium: KH
2PO
$0.5-5%, CaCl
20.01-0.5%, sodium acetate 0.2-2.0%, glucose 0.1-1.0%, peptone 0.1-1.5%, MnCl ﹒ 4H
2O 0.1-1.0, zero(ppm) water adds to 1L, pH6.8,10
5(Pa) sterilization 15-20min; Above per-cent is mass percent.
3) fermention medium: KH
2PO
$0.5-5%, CaCl
20.01-0.5%, sodium acetate 0.2-2.0%, glucose 1-10%, peptone 0.5-5%, zero(ppm) water adds to 1L, pH6.8,10
5The Pa 15-20min that sterilizes; Above per-cent is mass percent.
Cultivation of b, mikrobe and fermentation condition:
Said 1) culture presevation culture medium culturing condition and method for preserving: in test tube, add unpasteurized substratum; Account for test tube total amount 1/3; Sterilization back test tube vertically is placed on the test-tube stand, and a week is placed in the cooling back, confirm as aseptic after; Puncture inserts cultured bacterial classification in sterilisable chamber, 30
02-4 week is cultivated in C and illumination down; In the lighting box, with 40 watts electric light symmetrical illumination, intensity of illumination is good about with 1000 lxs; Cultivating sophisticated sign is to produce red bacterium colony around the puncture needle; Solid medium after the cultivation maturation adds in sterilisable chamber in the test tube with the MO of sterilization, and MO is higher than substratum 1cm, is placed on 4
0Preserve in the C refrigerator;
Said 2) seed culture medium culture condition: seed culture medium inserts the storage medium bacterial classification after sterilization, 25
0C-35
0C aerobic culture 15-40h, ventilation is than being 1:0.5-2.0;
Said 3) fermention medium fermentation condition: fermention medium inserts the bacterial classification of 5-15% seed culture medium after sterilization, 25
0C-35
0C aerobic culture 15-40h, ventilation is than being 1:0.5-2.0;
The extraction of c, flocculation agent:
Cultivate the fermented liquid after the maturation, at 5000-10000r/min spinning 20-40min, collect clear liquid and thalline with supercentrifuge respectively, said clear liquid is as the preparation liquid of flocculation agent, and thalline is capable of using as feed etc. as comprehensive utilization.
Collect above-mentioned clear liquid, add 1-3 absolute ethyl alcohol doubly, after mixing, static 8-10h in container, collecting precipitation thing, throw out collect solid again with 4000-8000r/min spinning 20-40min;
D, the preparation of flocculation agent finished product:
Above-mentioned solid vacuum-drying obtains flocculation agent white solid powder finished product, vacuum drying temperature: 60
0C ± 2
0C, vacuum tightness 0.06MPa.
The present invention has significant technique effect owing to adopted above technical scheme:
1, Rhodopseudomonas palustris of the present invention is affected by environment and make a variation, stable performance, and this bacterial classification institute produce flocculant storage property is good, under the normal temperature (38
0Below the C) store 1 year, the flocculation agent flocculation efficiency can reach more than 95%;
2, the Rhodopseudomonas palustris flocculation agent of producing with inoculum, method is easy, and fermentation period is short, and cost is low, is applicable to scale operation.
Embodiment
Below in conjunction with specific embodiment the present invention is described in further detail:
Embodiment 1
Select for use Rhodopseudomonas palustris (
Rhodop seudanonas palustris) be starting strain, culture presevation numbering: CGMCC1.2181, preservation place: Chinese common micro-organisms culture presevation administrative center;
The bacterium for producing flocculant culture process
1, substratum
1) seed culture medium: H
2PO
$1.5%, CaCl
20.02%, sodium acetate 1.0%, glucose 0.5 %, peptone 1%, MnCl ﹒ 4H
2O 0.7, and zero(ppm) water adds to 1L, pH6.8,10
5(Pa) sterilization 15min;
2) fermention medium: H
2PO
$2%, CaCl
20.03%, sodium acetate 1.5%, glucose 8.0%, peptone 3.5%, zero(ppm) water adds to 1L, pH6.8,10
5(Pa) sterilization 15min;
2, cultivation of mikrobe and fermentation condition
1) seed culture medium culture condition: seed culture medium inserts the storage medium bacterial classification after sterilization, 30
0C aerobic culture 18h, ventilation is than being 1:1;
2) fermention medium fermentation condition: fermention medium inserts the bacterial classification of 10% seed culture medium after sterilization, 30
0C aerobic culture 30h, ventilation is than being 1:1.2;
3, the extraction of flocculation agent
Cultivate the fermented liquid after the maturation, at 8000r/min spinning 30min, collect clear liquid and thalline with supercentrifuge respectively, clear liquid is as the preparation liquid of flocculation agent, and thalline is capable of using as feed etc. as comprehensive utilization.
Collect above-mentioned clear liquid, add 2.5 times absolute ethyl alcohol, after mixing, static 8h in container, collecting precipitation thing, throw out collect solid again with 6000r/min spinning 25min.
4, flocculation agent finished product preparation
Above-mentioned solid vacuum-drying obtains flocculation agent white solid powder finished product, vacuum drying temperature: 60
0C ± 2
0C, vacuum tightness 0.06MPa.
Embodiment 2
Select for use Rhodopseudomonas palustris (
Rhodop seudanonas palustris) be starting strain, culture presevation numbering: CGMCC1.2181, preservation place: Chinese common micro-organisms culture presevation administrative center;
The bacterium for producing flocculant culture process
1, substratum;
1) seed culture medium;
KH
2PO
$1.0%, CaCl
20.5%, sodium acetate 1.5%, glucose 0.8%, peptone 0.8%, MnCl ﹒ 4H
2O 0.5, and zero(ppm) water adds to 1L, pH7.0,10
5(Pa) sterilization 20min;
2) fermention medium;
KH
2PO
$0.5-5%, CaCl
20.01-0.5%, sodium acetate 0.2-2.0%, glucose 1-10%, peptone 0.5-5%, zero(ppm) water adds to 1L, pH6.8,10
5(Pa) sterilization 15-20min;
2, cultivation of mikrobe and fermentation condition
1) seed culture medium culture condition: seed culture medium inserts the storage medium bacterial classification after sterilization, 35
0C aerobic culture 25h, ventilation is than being 1:1.5;
2) fermention medium fermentation condition: fermention medium inserts the bacterial classification of 8% seed culture medium after sterilization, 35
0C aerobic culture 38h, ventilation is than being 1:1.5;
3, the extraction of flocculation agent
Cultivate the fermented liquid after the maturation, at 7000r/min spinning 35min, collect clear liquid and thalline with supercentrifuge respectively, clear liquid is as the preparation liquid of flocculation agent, and thalline is capable of using as feed etc. as comprehensive utilization.
Collect above-mentioned clear liquid, add 3 times absolute ethyl alcohol, after mixing, static 10h in container, collecting precipitation thing, throw out collect solid again with 5000r/min spinning 35min.
4, flocculation agent finished product preparation
Above-mentioned solid vacuum-drying obtains flocculation agent white solid powder finished product, vacuum drying temperature: 60
0C ± 2
0C, vacuum tightness 0.06MPa.
Embodiment 3
Select for use Rhodopseudomonas palustris (
Rhodop seudanonas palustris) be starting strain, culture presevation numbering: CGMCC1.2181, preservation place: Chinese common micro-organisms culture presevation administrative center;
The bacterium for producing flocculant culture process
1, substratum
1) seed culture medium;
KH
2PO
$2.5%, CaCl
20.5%, sodium acetate 1.8%, glucose 1.0%, peptone 1.2%, MnCl ﹒ 4H
2O 0.9, and zero(ppm) water adds to 1L, pH7.2,10
5(Pa) sterilization 20min;
2) fermention medium;
KH
2PO
$1.0%, CaCl
20.4%, sodium acetate 2.0%, glucose 7.5%, peptone 4.0%, zero(ppm) water adds to 1L, pH6.8,10
5(Pa) sterilization 20min;
2, cultivation of mikrobe and fermentation condition
1) seed culture medium culture condition: seed culture medium inserts the storage medium bacterial classification after sterilization, 28
0C aerobic culture 35h, ventilation is than being 1:1.2;
2) fermention medium fermentation condition: fermention medium inserts the bacterial classification of 12% seed culture medium after sterilization, 28
0C aerobic culture 40h, ventilation is than being 1:1.1;
3, the extraction of flocculation agent
Cultivate the fermented liquid after the maturation, at 6000r/min spinning 40min, collect clear liquid and thalline with supercentrifuge respectively, clear liquid is as the preparation liquid of flocculation agent, and thalline is capable of using as feed etc. as comprehensive utilization.
Collect above-mentioned clear liquid, add 1.5 times absolute ethyl alcohol, after mixing, static 10h in container, collecting precipitation thing, throw out collect solid again with 7000 r/min spinning 40min.
4, flocculation agent finished product preparation
Above-mentioned solid vacuum-drying obtains flocculation agent white solid powder finished product, vacuum drying temperature: 60
0C ± 2
0C, vacuum tightness 0.06MPa.
Microbial flocculant flocculating rate measuring method:
The 1%CaCl that in the 100mL tube comparison tubes, adds 0.5g kaolin, 3mL
2Solution and 2mL flocculation agent, adding distil water covers grinding port plug to 100mL then, and tube comparison tubes is overturn up and down and the 2min that vibrates naturally, must make kaolin and CaCl
2Solution and flocculation agent dissolve and thorough mixing fully, static 5min;
Get the treatment solution at 50mL place in the tube comparison tubes, with its absorbancy OD of 722 type spectrophotometric determinations in the 550nm wavelength
550(A), identical but replace the blank (B) as treatment solution with the 2mL fresh medium with other conditions, the calculation formula of flocculating rate (FR) is following:
FR(%)=(A-B)/A×100；
Through measuring, the flocculation efficiency of the flocculation agent of above-mentioned 3 embodiment all can reach more than 95%.
In a word, the above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to the covering scope of patent of the present invention.
Claims (1)
1. the preparation method of a photosynthetic bacterium flocculation agent is characterized in that, concrete grammar is following:
Select for use Rhodopseudomonas palustris (
Rhodop seudanonas palustris) be starting strain, culture presevation numbering: CGMCC1.2181, preservation place: Chinese common micro-organisms culture presevation administrative center;
A, preparing culture medium:
1) culture presevation substratum: (NH
4)
2SO
41g, MgSO
4﹒ 7H
2O 0.2g, NaHCO
35g, K
2HPO
40.5g, NaCl 0.2g, peptone 1.5g, zero(ppm) water adds to 1L, agar 15.0g, pH7.0; 10
5The Pa 15min that sterilizes;
2) seed culture medium: KH
2PO
$0.5-5%, CaCl
20.01-0.5%, sodium acetate 0.2-2.0%, glucose 0.1-1.0%, peptone 0.1-1.5%, MnCl ﹒ 4H
2O 0.1-1.0, zero(ppm) water adds to 1L, pH6.8,10
5(Pa) sterilization 15-20min;
3) fermention medium: KH
2PO
$0.5-5%, CaCl
20.01-0.5%, sodium acetate 0.2-2.0%, glucose 1-10%, peptone 0.5-5%, zero(ppm) water adds to 1L, pH6.8,10
5The Pa 15-20min that sterilizes;
Cultivation of b, mikrobe and fermentation condition:
Said 1) culture presevation culture medium culturing condition and method for preserving: in test tube, add unpasteurized substratum; Account for test tube total amount 1/3; Sterilization back test tube vertically is placed on the test-tube stand, and a week is placed in the cooling back, confirm as aseptic after; Puncture inserts cultured bacterial classification in sterilisable chamber, 30
02-4 week is cultivated in C and illumination down; In the lighting box, with 40 watts electric light symmetrical illumination, intensity of illumination is good about with 1000 lxs; Cultivating sophisticated sign is to produce red bacterium colony around the puncture needle; Solid medium after the cultivation maturation adds in sterilisable chamber in the test tube with the MO of sterilization, and MO is higher than substratum 1cm, is placed on 4
0Preserve in the C refrigerator;
Said 2) seed culture medium culture condition: seed culture medium inserts the storage medium bacterial classification after sterilization, 25
0C-35
0C aerobic culture 15-40h, ventilation is than being 1:0.5-2.0;
Said 3) fermention medium fermentation condition: fermention medium inserts the bacterial classification of 5-15% seed culture medium after sterilization, 25
0C-35
0C aerobic culture 15-40h, ventilation is than being 1:0.5-2.0;
The extraction of c, flocculation agent:
Fermented liquid after the cultivation maturation at 5000-10000r/min spinning 20-40min, is collected clear liquid and thalline with supercentrifuge respectively, and said clear liquid is as the preparation liquid of flocculation agent;
Collect above-mentioned clear liquid, add 1-3 absolute ethyl alcohol doubly, after mixing, static 8-10h in container, collecting precipitation thing, throw out collect solid again with 4000-8000r/min spinning 20-40min;
D, the preparation of flocculation agent finished product:
Above-mentioned solid vacuum-drying obtains flocculation agent white solid powder finished product, vacuum drying temperature: 60
0C ± 2
0C, vacuum tightness 0.06MPa.
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|---|---|---|---|
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ID=45690387
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649969A (en) * | 2012-04-13 | 2012-08-29 | 济南环亿生物科技有限公司 | Biological zinc preparation and method for preparing same |
| CN104004787A (en) * | 2014-05-28 | 2014-08-27 | 湖南大学 | Method for concentrating and separating biological flocculant from fermentation liquid |
| CN105039415A (en) * | 2015-06-25 | 2015-11-11 | 广东工业大学 | Method for synthesizing flocculant by utilizing Acinetobacter baumanii |
| CN106119144A (en) * | 2016-04-11 | 2016-11-16 | 江苏师范大学 | A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant |
| CN110835609A (en) * | 2018-08-16 | 2020-02-25 | 湛江恒兴养殖技术服务有限公司 | Photosynthetic bacteria culture medium and preparation method and application thereof |
| CN110982750A (en) * | 2019-12-26 | 2020-04-10 | 河北大河生物科技有限公司 | High-density fermentation method for rhodopseudomonas palustris and application of high-density fermentation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86107488A (en) * | 1985-11-05 | 1987-08-26 | 土壤技术公司 | The method of microbial composite and processing soil |
| CN1854289A (en) * | 2005-04-28 | 2006-11-01 | 中国水产科学研究院东海水产研究所 | Photosynthetic bacterial concentration |
-
2011
- 2011-10-25 CN CN201110326861.5A patent/CN102363793B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86107488A (en) * | 1985-11-05 | 1987-08-26 | 土壤技术公司 | The method of microbial composite and processing soil |
| CN1854289A (en) * | 2005-04-28 | 2006-11-01 | 中国水产科学研究院东海水产研究所 | Photosynthetic bacterial concentration |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649969A (en) * | 2012-04-13 | 2012-08-29 | 济南环亿生物科技有限公司 | Biological zinc preparation and method for preparing same |
| CN104004787A (en) * | 2014-05-28 | 2014-08-27 | 湖南大学 | Method for concentrating and separating biological flocculant from fermentation liquid |
| CN104004787B (en) * | 2014-05-28 | 2016-06-08 | 湖南大学 | A kind of method of concentrating and separating biological flocculant from fermentation liquid |
| CN105039415A (en) * | 2015-06-25 | 2015-11-11 | 广东工业大学 | Method for synthesizing flocculant by utilizing Acinetobacter baumanii |
| CN105039415B (en) * | 2015-06-25 | 2018-11-09 | 广东工业大学 | A method of utilizing Acinetobacter bauamnnii synthetic flocculant |
| CN106119144A (en) * | 2016-04-11 | 2016-11-16 | 江苏师范大学 | A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant |
| CN106119144B (en) * | 2016-04-11 | 2019-07-26 | 江苏师范大学 | A kind of conversion peanut shell hydrolysate prepares the method and bacterial strain of microbial flocculant |
| CN110835609A (en) * | 2018-08-16 | 2020-02-25 | 湛江恒兴养殖技术服务有限公司 | Photosynthetic bacteria culture medium and preparation method and application thereof |
| CN110982750A (en) * | 2019-12-26 | 2020-04-10 | 河北大河生物科技有限公司 | High-density fermentation method for rhodopseudomonas palustris and application of high-density fermentation method |
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|---|---|
| CN102363793B (en) | 2014-03-05 |
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Effective date of registration: 20161207 Address after: 310000 Xihu District, Hangzhou, Wen Wen Road No. 164, Hangzhou Institute of Commerce,, two Patentee after: ZHEJIANG SHUANGLIANG SHANGDA ENVIRONMENTAL PROTECTION Co.,Ltd. Address before: 310000, Hangzhou economic and Technological Development Zone, Zhejiang, Yuen Yuen Street, No. 68 Hangzhou vocational Park 4-5 building Patentee before: Hangzhou Jiangnan Science Research Institute Co.,Ltd. |
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Address after: Inside Hangzhou Business School, No.164 Wen'er Road, Xihu District, Hangzhou City, Zhejiang Province, 310000 Patentee after: Zhe JIANG Shuangliang Sunda Environment Protection Co.,Ltd. Address before: Inside Hangzhou Business School, No.164 Wen'er Road, Xihu District, Hangzhou City, Zhejiang Province, 310000 Patentee before: ZHEJIANG SHUANGLIANG SHANGDA ENVIRONMENTAL PROTECTION Co.,Ltd. |
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